PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 14614543-1 2003 To find an effective and quick way of purifying and identifying recombinant human IFN-beta (rhIFN-beta) expressed in yeast Pichia pastoris, Blue Sepharose 6 fast flow (Blue S6FF) and immunological affinity chromatography (IAC) were compared in this report. Sepharose 145-154 interferon beta 1 Homo sapiens 82-90 6733118-0 1984 Elution of interferon beta from blue sepharose by poly(vinylpyrrolidone). Sepharose 37-46 interferon beta 1 Homo sapiens 11-26 10705228-3 2000 The anti-rspg41 antisera after adsorption by IFN-beta sepharose column lost the activity of interaction with both synthetic peptides. Sepharose 54-63 interferon beta 1 Homo sapiens 45-53 10705228-4 2000 In another experiment, rsgp41 could bind to sepharose column conjugated with anti-IFN-beta polyclonal antibodies (IgG). Sepharose 44-53 interferon beta 1 Homo sapiens 82-90 6170512-2 1981 The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon-beta separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and absorbs interferon-beta immunologically when bound to CNBr-activated Sepharose. Sepharose 355-364 interferon beta 1 Homo sapiens 54-75 6170512-2 1981 The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon-beta separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and absorbs interferon-beta immunologically when bound to CNBr-activated Sepharose. Sepharose 355-364 interferon beta 1 Homo sapiens 154-169 6170512-2 1981 The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon-beta separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and absorbs interferon-beta immunologically when bound to CNBr-activated Sepharose. Sepharose 355-364 interferon beta 1 Homo sapiens 294-309 6155666-1 1980 Human fibroblast interferon was partially purified, about 4,000-fold, on a chromatographic tandem of columns: concanavalin A-agarose leads to phenyl-agarose, to a specific activity of ca 4 x 10(7). Sepharose 125-132 interferon beta 1 Homo sapiens 6-27 7352259-1 1980 The purification of human fibroblast interferon has been simplified to a two-step procedure consisting of affinity chromatography on Blue Sepharose and sodium dodecyl sulfate polyacrlamide gel electrophoresis. Sepharose 138-147 interferon beta 1 Homo sapiens 26-47 27163-0 1978 Stabilization of human fibroblast interferon purified on concanavalin A-agarose. Sepharose 72-79 interferon beta 1 Homo sapiens 23-44 27163-1 1978 Human fibroblast interferon, obtained by chromatography on concanavalin A-agarose, was stable for at least a month in 30--50 per cent ethylene glycol at 4 degrees, --20 degrees, and --70 degrees C. The succinct point of the present finding is that human fibroblast interferon may be stabilized by ethylene glycol alone without the addition of bovine serum albumin and "back-contamination" of the interferon preparation. Sepharose 74-81 interferon beta 1 Homo sapiens 6-27 956190-1 1976 Human fibroblast interferon binds to L-tryptophan, D-tryptophan, L-phenylalanine, and L-tyrosine, all immobilized directly to cyanogen bromide-activated agarose, as well as to L-tryptophan and D-tryptophan methyl ester, both immobilized via molecular arms. Sepharose 153-160 interferon beta 1 Homo sapiens 6-27