PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
8894274-2	1996	These results were confirmed by affinity chromatography experiments demonstrating that S100A1 and S100B bound to phosphoglucomutase-Sepharose in a calcium-dependent manner.	Sepharose	132-141	S100 calcium binding protein B	Homo sapiens	98-103	
8894274-3	1996	In the reverse experiment, phosphoglucomutase bound to S100A1 and S100B-Sepharose in a calcium-dependent manner.	Sepharose	72-81	S100 calcium binding protein B	Homo sapiens	66-71	
7540194-5	1995	After incubation with a Jurkat cell lysate, the GST-Nef constructs immobilized on glutathione-agarose beads bound to cellular kinase(s) and were phosphorylated at three sites in vitro: one on threonine at position 15, one on serine between residues 1 and 35, and one on threonine between residues 36 and 86.	Sepharose	94-101	S100 calcium binding protein B	Homo sapiens	52-55	
9049329-2	1995	The ability of GST-Nef to act as a substrate for cellular kinases in vitro was examined by incubation of purified GST-Nef fusion proteins, immobilized on glutathione-agarose beads, with cytoplasmic extracts from a number of human cell lines.	Sepharose	166-173	S100 calcium binding protein B	Homo sapiens	19-22	
8126463-4	1994	Large-scale production of E. coli-derived Nef 27 and Nef 25 was carried out by growing recombinant cells in a fermenter under fed-batch conditions followed by affinity purification on glutathione-Sepharose before and after thrombin cleavage.	Sepharose	196-205	S100 calcium binding protein B	Homo sapiens	42-45	
3203499-6	1988	IgG subclass distribution of NEF antibody sera was also investigated by means of protein A-Sepharose and using monoclonal antibodies against human IgG subclasses.	Sepharose	91-100	S100 calcium binding protein B	Homo sapiens	29-32	
6733116-3	1984	Calmodulin and S-100 protein were eluted from the aminoocytl -agarose column with 1 mM EGTA in the presence of 0.15 M NaCl and the elution of troponin-C was Ca2+-independently carried out with 0.3 M NaCl.	Sepharose	62-69	S100 calcium binding protein B	Homo sapiens	15-20	
739266-2	1978	In the present study this is shown also by incubating neurons with Sepharose 4B or methylacrylate spherules to which were coupled anti-S-100 antibodies.	Sepharose	67-79	S100 calcium binding protein B	Homo sapiens	135-140