PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1704234-1 1990 A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column. Sepharose CL 4B 203-218 alpha fetoprotein Homo sapiens 44-47 1704234-1 1990 A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column. Sepharose CL 4B 203-218 alpha fetoprotein Homo sapiens 57-74 1704234-1 1990 A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column. Sepharose CL 4B 203-218 alpha fetoprotein Homo sapiens 76-79 1704234-3 1990 The immunotoxin, anti-AFP-abrin-A conjugate, which was also purified with a protein A-sepharose CL-4B affinity column, had a molecular weight of 180,000 and had 80% antigen-binding activity that of anti-AFP activity and 92% toxicity of abrin-A chain. Sepharose CL 4B 86-101 alpha fetoprotein Homo sapiens 22-25