PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3025197-2 1987 Deoxyuridine triphosphatase (dUTPase), an enzyme that catalyzes hydrolysis of dUTP to deoxyuridylate and inorganic pyrophosphate, has been purified approximately 6,000-fold from Drosophila embryos. deoxyuridine triphosphate 29-33 Deoxyuridine triphosphatase Drosophila melanogaster 0-27 6134653-12 1983 Strains carrying such a shortened dut-pHUB2 derivative and a temperature-sensitive lambda repressor overproduced dUTPase very dramatically after heat induction. deoxyuridine triphosphate 34-37 Deoxyuridine triphosphatase Drosophila melanogaster 113-120 6139280-1 1983 The nucleotide sequence of the dUTPase structural gene, dut, of Escherichia coli has been determined. deoxyuridine triphosphate 56-59 Deoxyuridine triphosphatase Drosophila melanogaster 31-38 148458-3 1978 One mutant produced a thermolabile enzyme, and it was presumed to have a mutation in the structural gene for dUTPase, designated dut. deoxyuridine triphosphate 129-132 Deoxyuridine triphosphatase Drosophila melanogaster 109-116 810487-10 1975 We propose that infection at high pH prevents the PBS2-induced dUTPase inhibitor from blocking the B. subtilis dUTPase activity, thereby allowing the degradation of dUTP and the synthesis of dTTP (both of which are DNA polymerase substrates), so that thymine replaces some of the uracil normally found in PBS2 DNA. deoxyuridine triphosphate 63-67 Deoxyuridine triphosphatase Drosophila melanogaster 111-118 31662460-7 2019 Our crystallographic and biochemical studies reveal that ASFV E165R is a member of trimeric dUTP nucleotidohydrolase (dUTPase) family that catalyzes the hydrolysis of dUTP into dUMP. deoxyuridine triphosphate 92-96 Deoxyuridine triphosphatase Drosophila melanogaster 118-125 30987342-2 2019 Deoxyuridine 5"-triphosphate nucleotidohydrolase (dUTPase) is a key enzyme in this pathway since it catalyzes the cleavage of 2"-deoxyuridine 5"-triphosphate (dUTP) into 2"-deoxyuridine 5"-monophosphate (dUMP) and inorganic pyrophosphate. deoxyuridine triphosphate 126-157 Deoxyuridine triphosphatase Drosophila melanogaster 50-57 30987342-6 2019 We find that heterozygous dut +/- animals are viable while having decreased dUTPase levels. deoxyuridine triphosphate 26-29 Deoxyuridine triphosphatase Drosophila melanogaster 76-83 30987342-7 2019 Importantly, we show that dUTPase is essential for embryonic development since early dut -/- embryos reach the blastocyst stage, however, they die shortly after implantation. deoxyuridine triphosphate 85-88 Deoxyuridine triphosphatase Drosophila melanogaster 26-33 30902068-2 2019 Knockdown of the DUT gene in Dr, which encodes deoxyuridine 5"-triphosphate pyrophosphatase (dUTPase), promotes DNA fragmentation, inhibits regeneration, and eventually leads to death. deoxyuridine triphosphate 17-20 Deoxyuridine triphosphatase Drosophila melanogaster 93-100 30902068-9 2019 We observed the hydrolysis of dUTP by Dr-dUTPase using Cresol Red as a proton sensor. deoxyuridine triphosphate 30-34 Deoxyuridine triphosphatase Drosophila melanogaster 41-48 30902068-10 2019 The Km for dUTP was determined to be 4.0 microM, which is similar to that for human dUTPase. deoxyuridine triphosphate 11-15 Deoxyuridine triphosphatase Drosophila melanogaster 84-91 28423595-4 2017 Interestingly, these effects can be reinforced by either depletion or inhibition of the deoxyuridine triphosphatase (dUTPase, also known as DUT), highlighting the importance of (5-F)dUTP accumulation for cytotoxicity.With this study, we not only extend the current understanding of the mechanism of action of 5-FU, but also contribute to the characterization of dUTPase inhibitors. deoxyuridine triphosphate 117-121 Deoxyuridine triphosphatase Drosophila melanogaster 362-369 27933035-13 2016 Our data indicate that several unicellular microorganisms may efficiently cope with a dut- genotype lacking all of the previously described dUTPase genes, and potentially leading to an unusual uracil-enrichment in their genomic DNA. deoxyuridine triphosphate 86-89 Deoxyuridine triphosphatase Drosophila melanogaster 140-147 26208523-3 2015 In the p53-proficient colorectal cancer cell line HCT116, oxaliplatin represses the expression of deoxyuridine triphosphatase (dUTPase), a ubiquitous pyrophosphatase that catalyzes the hydrolysis of dUTP to dUMP and inhibits dUTP-mediated cytotoxicity. deoxyuridine triphosphate 199-203 Deoxyuridine triphosphatase Drosophila melanogaster 127-134 25841100-7 2015 We also found that the expression of the Stl protein in Mycobacterium smegmatis led to highly increased cellular dUTP levels in the mycobacterial cell, this effect being in agreement with its dUTPase inhibitory role. deoxyuridine triphosphate 113-117 Deoxyuridine triphosphatase Drosophila melanogaster 192-199 25735890-1 2015 dUTP pyrophosphatase (dUTPase) is a dNTP-sanitizing enzyme that prevents the appearance of potentially harmful uracil bases in DNA by hydrolyzing cellular dUTP. deoxyuridine triphosphate 0-4 Deoxyuridine triphosphatase Drosophila melanogaster 22-29 25274731-6 2014 We also show that dUTPase enzymatic activity is strongly inhibited in the dUTPase:Stl complex and that the dUTPase:dUTP complex is inaccessible to the Stl repressor. deoxyuridine triphosphate 18-22 Deoxyuridine triphosphatase Drosophila melanogaster 74-81 25274731-6 2014 We also show that dUTPase enzymatic activity is strongly inhibited in the dUTPase:Stl complex and that the dUTPase:dUTP complex is inaccessible to the Stl repressor. deoxyuridine triphosphate 18-22 Deoxyuridine triphosphatase Drosophila melanogaster 74-81 23307478-1 2014 Nuclear-associated deoxyuridine 5"-triphosphate nucleotidohydrolase (dUTPase) is an enzyme that hydrolyses deoxyuridine 5"-triphosphate (dUTP) to the monophosphate, thereby controlling the dUTP levels of the organism, which is essential for survival. deoxyuridine triphosphate 19-47 Deoxyuridine triphosphatase Drosophila melanogaster 69-76 23307478-1 2014 Nuclear-associated deoxyuridine 5"-triphosphate nucleotidohydrolase (dUTPase) is an enzyme that hydrolyses deoxyuridine 5"-triphosphate (dUTP) to the monophosphate, thereby controlling the dUTP levels of the organism, which is essential for survival. deoxyuridine triphosphate 137-141 Deoxyuridine triphosphatase Drosophila melanogaster 69-76 23982515-3 2013 Here, using the Mason-Pfizer monkey retrovirus dUTPase, we study the dUTPase-catalyzed hydrolysis of dUTP, an incorrect DNA building block, to elaborate the mechanistic details at high resolution. deoxyuridine triphosphate 47-51 Deoxyuridine triphosphatase Drosophila melanogaster 69-76 23201394-4 2013 We first generated conditional DUT-knockout strains that allowed an effective decrease of dUTPase resulting in proliferation arrest, although gene repression could not be sustained long enough to cause lethality. deoxyuridine triphosphate 31-34 Deoxyuridine triphosphatase Drosophila melanogaster 90-97 23040389-7 2012 The half-life of the enzyme at 94 C was about 7 h. The specific activity of the Tpa dUTPase on dUTP was about 10-20-fold higher than that of Tpa dUTPase on dCTP. deoxyuridine triphosphate 85-89 Deoxyuridine triphosphatase Drosophila melanogaster 146-153 21145358-6 2011 Given that the function of dUTPase is to decrease the levels of dUTP, our results strongly support an emerging role for dUTP as a pro-apoptotic second messenger in the same vein as ROS and ceramide. deoxyuridine triphosphate 64-68 Deoxyuridine triphosphatase Drosophila melanogaster 27-34 19342774-1 2009 All organisms examined to date possess a dUTPase that performs the important function of efficiently hydrolyzing dUTP to dUMP in order to prevent the incorporation of dUTP into DNA. deoxyuridine triphosphate 113-117 Deoxyuridine triphosphatase Drosophila melanogaster 41-48 18619713-1 2009 The ubiquitous enzyme dUTP nucleotidohydrolase (dUTPase) catalyses the hydrolysis of dUTP to dUMP and can be considered as the first line of defence against incorporation of uracil into DNA. deoxyuridine triphosphate 22-26 Deoxyuridine triphosphatase Drosophila melanogaster 48-55 18837522-8 2009 To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase (dUTPase) enzyme family eliminate excess dUTP. deoxyuridine triphosphate 65-69 Deoxyuridine triphosphatase Drosophila melanogaster 91-98 19055736-3 2008 Deoxyuridine triphosphatase (dUTPase) hydrolyzes dUTP, generating dUMP for biosynthesis of thymidine nucleotides while decreasing the availability of dUTP for misincorporation; uracil DNA glycosylase (UNG) cleaves uracil N-glycosylic bonds in DNA initiating base excision repair. deoxyuridine triphosphate 49-53 Deoxyuridine triphosphatase Drosophila melanogaster 29-36 18586121-0 2008 Kinetic and thermodynamic characterization of dUTP hydrolysis by Plasmodium falciparum dUTPase. deoxyuridine triphosphate 46-50 Deoxyuridine triphosphatase Drosophila melanogaster 87-94 18586121-1 2008 Deoxyuridine 5"-triphosphate nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate and plays an important role in nucleotide metabolism and DNA replication controlling relative cellular levels of dTTP/dUTP, both of which can be incorporated into DNA. deoxyuridine triphosphate 87-91 Deoxyuridine triphosphatase Drosophila melanogaster 50-57 18790783-3 2008 dUTP nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and PPi, providing substrate for thymidylate synthase (TS) and DNA synthesis and repair. deoxyuridine triphosphate 0-4 Deoxyuridine triphosphatase Drosophila melanogaster 26-33 17459559-0 2007 Effect of an Asp80Ala substitution on the binding of dUTP and dUMP to Trypanosoma cruzi dUTPase. deoxyuridine triphosphate 53-57 Deoxyuridine triphosphatase Drosophila melanogaster 88-95 17459559-2 2007 The thermodynamics of binding for both dUTP and dUMP (deoxyuridine 5"-monophosphate) to the D80A mutant form of Trypanosoma cruzi dUTPase have been investigated by fluorescence spectroscopy and high-sensitivity isothermal titration calorimetry. deoxyuridine triphosphate 39-43 Deoxyuridine triphosphatase Drosophila melanogaster 130-137 15629463-1 2005 Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. deoxyuridine triphosphate 115-119 Deoxyuridine triphosphatase Drosophila melanogaster 47-54 15629463-3 2005 dUTPase activity was reduced approximately 95+/-5% in all cell lines tested 48 h after transfection with 2 microg siRNAdUT3 and it was maintained at this decreased level for at least 72 h. Down-regulation of dUTPase resulted in a significant increase in intracellular dUTP and a decreased proliferation of the transfected cells. deoxyuridine triphosphate 0-4 Deoxyuridine triphosphatase Drosophila melanogaster 208-215 15276840-1 2004 The structure of Mycobacterium tuberculosis dUTP nucleotidohydrolase (dUTPase) has been determined at 1.3 Angstrom resolution in complex with magnesium ion and the non-hydrolyzable substrate analog, alpha,beta-imido dUTP. deoxyuridine triphosphate 44-48 Deoxyuridine triphosphatase Drosophila melanogaster 70-77 15971611-6 2004 The results demonstrate that the cells co-transfected with dUTPase and hMT-3 genes have more strong resistibility to dUTP than the cells transfected only with dUTPase gene. deoxyuridine triphosphate 59-63 Deoxyuridine triphosphatase Drosophila melanogaster 159-166 15971611-7 2004 And that the hMT-3 protein can accelerate the hydrolyzation of dUTP by dUTPase. deoxyuridine triphosphate 63-67 Deoxyuridine triphosphatase Drosophila melanogaster 71-78 12799180-1 2003 dUTP nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate and is the central regulator of cellular dUTP pools. deoxyuridine triphosphate 0-4 Deoxyuridine triphosphatase Drosophila melanogaster 26-33 12369926-2 2001 Prompt and specific removal of dUTP from the nucleotide pool, as expedited by the ubiquitous enzyme dUTPase, is therefore required for full viability in most biological systems. deoxyuridine triphosphate 31-35 Deoxyuridine triphosphatase Drosophila melanogaster 100-107 12374095-10 2001 dUTPase catalyzes the hydrolysis of dUTP to form dUMP and pyrophosphate thereby eliminating dUTP and preventing its utilization by DNA polymerases during replication and repair. deoxyuridine triphosphate 36-40 Deoxyuridine triphosphatase Drosophila melanogaster 0-7 12374095-13 2001 However, during TS inhibition, dUTP pools may accumulate and overwhelm dUTPase, resulting in repeated cycles of uracil misincorporation and detrimental repair leading to strand breaks and cell death. deoxyuridine triphosphate 31-35 Deoxyuridine triphosphatase Drosophila melanogaster 71-78 12374098-3 2001 Trypanosomatid genes coding for enzymes which distinctively hydrolyze dUTP have been isolated by genetic complementation in Escherichia coli mutants defective in dUTPase activity. deoxyuridine triphosphate 70-74 Deoxyuridine triphosphatase Drosophila melanogaster 162-169 11487279-3 2001 The expansion in dUTP pools is largely determined by the expression of the pyrophosphatase, dUTPase. deoxyuridine triphosphate 17-21 Deoxyuridine triphosphatase Drosophila melanogaster 92-99 10957629-1 2000 dUTP pyrophosphatase (dUTPase) cleaves the alpha-beta phosphodiester of dUTP to form pyrophosphate and dUMP, preventing incorporation of uracil into DNA and providing the substrate for thymine synthesis. deoxyuridine triphosphate 0-4 Deoxyuridine triphosphatase Drosophila melanogaster 22-29 10952785-2 2000 dUTPase catalyses the hydrolysis of dUTP to dUMP, thereby maintaining low intracellular dUTP. deoxyuridine triphosphate 36-40 Deoxyuridine triphosphatase Drosophila melanogaster 0-7 10952785-9 2000 There was a striking variation in the amount of dUTP formed following exposure to ZD9331 (between 1.3 and 57 pmole 10(-6)cells) and was in general inversely associated with dUTPase activity. deoxyuridine triphosphate 48-52 Deoxyuridine triphosphatase Drosophila melanogaster 173-180 10952785-10 2000 A large expansion in the dUTP pool was associated with increased sensitivity to a 24-h exposure to ZD9331 in A549 cells that have low dUTPase activity/expression. deoxyuridine triphosphate 25-29 Deoxyuridine triphosphatase Drosophila melanogaster 134-141 10910061-2 2000 dUTP nucleotidohydrolase (dUTPase) is the key regulator of dUTP pools, and significant evidence exists suggesting that the expression of this enzyme may be an important determinant of cytotoxicity mediated by inhibitors of thymidylate synthase (TS). deoxyuridine triphosphate 0-4 Deoxyuridine triphosphatase Drosophila melanogaster 26-33 10657253-4 2000 The enzyme has proved to be a dimer by gel filtration and is able to hydrolyse both dUTP and dUDP quite efficiently, acting as a dUTP nucleotidohydrolase (dUTPase)-dUDP nucleotidohydrolase but has a limited capacity to act upon other nucleoside di- or triphosphates. deoxyuridine triphosphate 84-88 Deoxyuridine triphosphatase Drosophila melanogaster 155-162 10329142-8 1999 We found that dUTP photoaffinity labelling was specific for a beta-hairpin motif in HERV-K dUTPase. deoxyuridine triphosphate 14-18 Deoxyuridine triphosphatase Drosophila melanogaster 91-98 9360704-2 1997 While considerable effort has been directed towards discovering new, more potent inhibitors of these two enzymes, little attention has been given dUTP pyrophosphatase (dUTPase)--the key modulator of cellular dUTP levels--as a potential target for chemotherapeutic drug development. deoxyuridine triphosphate 146-150 Deoxyuridine triphosphatase Drosophila melanogaster 168-175 8805593-1 1996 BACKGROUND: The essential enzyme dUTP pyrophosphatase (dUTPase) is exquisitely specific for dUTP and is critical for the fidelity of DNA replication and repair. deoxyuridine triphosphate 33-37 Deoxyuridine triphosphatase Drosophila melanogaster 55-62 8805593-2 1996 dUTPase hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing dUTP levels and providing the dUMP for dTTP biosynthesis. deoxyuridine triphosphate 19-23 Deoxyuridine triphosphatase Drosophila melanogaster 0-7 8735843-6 1996 Replacement of the alpha, beta-bridging oxygen in dUTP with an imido group resulted in a nonhydrolyzable substrate analogue and a potent competitive inhibitor of dUTPase (Ki = 5 microM). deoxyuridine triphosphate 50-54 Deoxyuridine triphosphatase Drosophila melanogaster 162-169 8551582-1 1996 The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measure the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. deoxyuridine triphosphate 22-26 Deoxyuridine triphosphatase Drosophila melanogaster 178-185 9104494-1 1996 The enzyme dUTP pyrophosphatase (dUTPase, EC 3.6.1.23) is essential for cellular DNA replication and cell viability by virtue of its role in reducing the availability of dUTP as a substrate for DNA polymerases. deoxyuridine triphosphate 11-15 Deoxyuridine triphosphatase Drosophila melanogaster 33-40 8162567-1 1994 Recent studies from our laboratory suggested that, in some human colorectal tumor cell lines, sensitivity to fluorodeoxyuridine may depend upon the extent of dUTP accumulation that occurs following drug treatment and that elevation of dUTPase activity might be the basis for some instances of resistance to fluoropyrimidines. deoxyuridine triphosphate 158-162 Deoxyuridine triphosphatase Drosophila melanogaster 235-242 8223452-1 1993 dUTP pyrophosphatase (dUTPase; EC 3.6.1.23) catalyses the hydrolysis of dUTP to dUMP and PPi and thereby prevents the incorporation of uracil into DNA during replication. deoxyuridine triphosphate 0-4 Deoxyuridine triphosphatase Drosophila melanogaster 22-29 1311056-1 1992 The enzyme dUTPase catalyses the hydrolysis of dUTP and maintains a low intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. deoxyuridine triphosphate 47-51 Deoxyuridine triphosphatase Drosophila melanogaster 11-18 2153555-1 1990 Deoxyuridine triphosphate nucleotidohydrolase (dUTPase), a key enzyme in pyrimidine nucleotide metabolism, specifically hydrolyzes deoxyuridine triphosphate (dUTP) to deoxyuridine monophosphate and inorganic pyrophosphate. deoxyuridine triphosphate 131-156 Deoxyuridine triphosphatase Drosophila melanogaster 47-54 33824328-3 2021 dUTPase catalyses the hydrolytic dephosphorylation of deoxyuridine triphosphate (dUTP) to deoxyuridine monophosphate (dUMP), providing dUMP for thymidylate synthase as part of the thymidylate biosynthesis pathway and maintaining low intracellular dUTP concentrations. deoxyuridine triphosphate 54-79 Deoxyuridine triphosphatase Drosophila melanogaster 0-7 33824328-3 2021 dUTPase catalyses the hydrolytic dephosphorylation of deoxyuridine triphosphate (dUTP) to deoxyuridine monophosphate (dUMP), providing dUMP for thymidylate synthase as part of the thymidylate biosynthesis pathway and maintaining low intracellular dUTP concentrations. deoxyuridine triphosphate 81-85 Deoxyuridine triphosphatase Drosophila melanogaster 0-7 34777521-1 2021 Background: Deoxyuracil triphosphate nucleotide (dUTP) pyrophosphatase (dUTPase, DU) is an enzyme of caprine arthritis-encephalitis virus (CAEV) that minimizes incorporation of dUTP into the DNA. deoxyuridine triphosphate 49-53 Deoxyuridine triphosphatase Drosophila melanogaster 72-79 34777521-1 2021 Background: Deoxyuracil triphosphate nucleotide (dUTP) pyrophosphatase (dUTPase, DU) is an enzyme of caprine arthritis-encephalitis virus (CAEV) that minimizes incorporation of dUTP into the DNA. deoxyuridine triphosphate 177-181 Deoxyuridine triphosphatase Drosophila melanogaster 72-79 35568200-5 2022 This efficient substrate discrimination is in line with the lack of thymidylate synthase and dUTPase in the parasite, which makes dUMP a dead-end product that is potentially harmful if converted to dUTP. deoxyuridine triphosphate 198-202 Deoxyuridine triphosphatase Drosophila melanogaster 93-100