PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7317405-8 1981 However, in hypotonic media (less than 400 mosmol/kg) or in Triton X-100 (0.2% final concentration) acetylcholinesterase activity was markedly higher. Octoxynol 60-72 acetylcholinesterase (Cartwright blood group) Homo sapiens 100-120 7459107-2 1980 The recovery of enzymatic activity when AChE wa solubilized by means of freezing and thawing followed by treatment with 0,5% Triton X-100, represented approximately 95% of the total erythricytic activity for both human and rat preparations. Octoxynol 125-137 acetylcholinesterase (Cartwright blood group) Homo sapiens 40-44 656488-1 1978 Gel-filtration of 0,6 M NaCl and 0,6 M NaCl--0,1% Triton X-100 extracts of freshly isolated sarcolemma through Sepharose 2B (1,5 X 72 cm) has revealed one symmetric peak of acetylcholinesterase activity containing phospholipid and cholesterol, moving faster than fibrinogen and tyreoglobulin. Octoxynol 50-62 acetylcholinesterase (Cartwright blood group) Homo sapiens 173-193 488916-4 1979 It was shown that acetylcholinesterase crosslinked in absence of Triton X-100 consists of molecular forms built up by dimeric protomers. Octoxynol 65-77 acetylcholinesterase (Cartwright blood group) Homo sapiens 18-38 623848-0 1978 [Effect of Triton X-100 on properties of acetylcholinesterase from human erythrocytes]. Octoxynol 11-23 acetylcholinesterase (Cartwright blood group) Homo sapiens 41-61 623848-1 1978 The effect of Triton X-100 on catalytic properties of acetylcholinesterase from human erythrocytes under acetylcholine hydrolysis, on sensitivity of acetylcholinesterase to specific phosphoorganic inhibitors and eserine, and on the mobility and isoenyme spectrum under analytical electrophoresis in polyacrylamide gel is investigated. Octoxynol 14-26 acetylcholinesterase (Cartwright blood group) Homo sapiens 54-74 623848-3 1978 The inhibitory effect of Triton X-100 is mainly competitive, 0.5% Triton X-100 decreases bimolecular constant (kII) of the interaction of acetylcholinesterase with phosphoorganic inhibitor and eserine in 2.5-4 times. Octoxynol 25-37 acetylcholinesterase (Cartwright blood group) Homo sapiens 138-158 623848-3 1978 The inhibitory effect of Triton X-100 is mainly competitive, 0.5% Triton X-100 decreases bimolecular constant (kII) of the interaction of acetylcholinesterase with phosphoorganic inhibitor and eserine in 2.5-4 times. Octoxynol 66-78 acetylcholinesterase (Cartwright blood group) Homo sapiens 138-158 623848-6 1978 The introduction of 0.1% Triton X-100 into polyacrylamide gel results in considerable quantitative redistribution of acetylcholinesterase isoenzyme fractions and in the change of the mobility of one fraction under electrophoresis. Octoxynol 25-37 acetylcholinesterase (Cartwright blood group) Homo sapiens 117-137 939223-2 1976 Acetylcholinesterase (EAChE; acetylcholine acetyl hydrolase, EC 3.1.1.7) was prepared from the erythrocyte membrane of Chinese adult males, and solubilised with 1% Triton X-100 in 20 mmol/l phosphate buffer, pH 7.4. Octoxynol 164-176 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 1181266-1 1975 The acetylcholinesterase from human erythrocytes was released from the plasma membrane with 0.2% Triton X-100 at low ionic strength and purified by two affinity chromatography steps on Sepharose-bound m-[6-(6-amino-caproylamino)caproylamino]phenyltrimethyl-ammonium. Octoxynol 97-109 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-24 4733238-2 1973 Acetylcholinesterase from human erythrocytes was solubilized with Triton X-100 in strong salt solution and partially purified by (NH(4))(2)SO(4) fractionation. Octoxynol 66-78 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 5073767-0 1972 Activation of acetylcholinesterase by Triton X-100. Octoxynol 38-50 acetylcholinesterase (Cartwright blood group) Homo sapiens 14-34 15143516-2 2003 The activity of acetylcholinesterase in suspension of cells compounds is 9.8 +/- 0.2 mumol of tiocholinbromide/mg protein/hour and is reduced under influence of exogenous ATP, NO2-, H2O2 and Triton X-100. Octoxynol 191-203 acetylcholinesterase (Cartwright blood group) Homo sapiens 16-36 5144280-0 1971 Solubilization of acetylcholinesterase from human erythrocytes by Triton X-100 in potassium chloride solution. Octoxynol 66-78 acetylcholinesterase (Cartwright blood group) Homo sapiens 18-38 29172437-5 2018 AChE was solubilized from frozen RBC by addition of 1% Triton X-100. Octoxynol 55-67 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 28892361-7 2017 AChE was solubilized from frozen RBCs with 1% (v/v) Triton X-100. Octoxynol 52-64 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 29066970-6 2017 The membrane bound AChE, solubilized from human RBC with 0.6% Triton X-100, binds to Hupresin and remains bound during washing with sodium chloride. Octoxynol 62-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-23 29066970-12 2017 A similar trial was conducted with red blood cell AChE in 0.6% Triton X-100. Octoxynol 63-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 50-54 20147288-5 2010 In NG108-15 neuroblastoma cells transfected with cDNAs encoding AChE(T) and PRiMA, PRiMA-linked G(4) AChE was found in membrane rafts and showed the same sensitivity to cold Triton X-100 extraction as in the brain. Octoxynol 174-186 acetylcholinesterase (Cartwright blood group) Homo sapiens 101-105 20147288-8 2010 The cytoplasmic domain of PRiMA, which differs between PRiMA I and PRiMA II, appeared to play some role in stabilizing the raft localization of G(4) AChE, because the Triton X-100-resistant fraction was smaller with the shorter PRiMA II isoform than that with the longer PRiMA I isoform. Octoxynol 167-179 acetylcholinesterase (Cartwright blood group) Homo sapiens 149-153 1654884-1 1991 Membrane-bound acetylcholinesterase (AChE) from the human erythrocyte is inhibited by chlorpromazine (CPZ) in a concentration range within this amphiphilic drug has been demonstrated to interact with erythrocyte membranes, causing a large spectrum of physical and structural effects; membrane solubilization with 0.5% Triton X-100 results in a complete loss of CPZ inhibitory potency. Octoxynol 318-330 acetylcholinesterase (Cartwright blood group) Homo sapiens 15-35 11568364-5 2001 While mammalian acetylcholinesterase activity has often been determined in tissue homogenates in the presence of the nondenaturing detergent Triton X-100 at a concentration of 1%, the potential actions of this detergent on the activity of this critical enzyme are not understood. Octoxynol 141-153 acetylcholinesterase (Cartwright blood group) Homo sapiens 16-36 1654884-1 1991 Membrane-bound acetylcholinesterase (AChE) from the human erythrocyte is inhibited by chlorpromazine (CPZ) in a concentration range within this amphiphilic drug has been demonstrated to interact with erythrocyte membranes, causing a large spectrum of physical and structural effects; membrane solubilization with 0.5% Triton X-100 results in a complete loss of CPZ inhibitory potency. Octoxynol 318-330 acetylcholinesterase (Cartwright blood group) Homo sapiens 37-41 2298208-4 1990 In Triton X-100 extracts of the subline K562-243, phosphatidylinositol-specific phospholipase C (PtdIns-PLC) from Bacillus thuringiensis converted most of the G2a AChE into a hydrophilic dimer (G2h), indicating that the G2a form possessed a hydrophobic glycoinositol phospholipid that mediated its attachment to the membrane. Octoxynol 3-15 acetylcholinesterase (Cartwright blood group) Homo sapiens 163-167 2194939-4 1990 Solubilization of normal, diabetic and insulin treated diabetic red cell membranes with Triton X-100 (0.2% v/v) caused a general decline in AChE activity, however the per cent decline in activity of diabetic enzyme was lower as compared to normal and insulin treated conditions. Octoxynol 88-100 acetylcholinesterase (Cartwright blood group) Homo sapiens 140-144 2583490-1 1989 Acetylcholinesterase from human erythrocytes solubilized by 1% Triton X-100 was resolved using polyacrylamide gel electrophoresis into two components. Octoxynol 63-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 2546574-1 1989 The activities of acetylcholinesterase and Ca2+ + Mg2+ ATPase were measured following treatment of human erythrocyte membranes with nonsolubilizing and solubilizing concentrations of Triton X-100. Octoxynol 183-195 acetylcholinesterase (Cartwright blood group) Homo sapiens 18-38 3912371-5 1985 This material was used to raise monoclonal anti-AchE antibodies which, when applied to immunosorbent chromatography of washed Triton X-100-lysed erythrocytes in one step, allowed a 246,000-fold purification of AchE with a yield of 88% and a specific activity of 3800 IU/mg. Octoxynol 126-138 acetylcholinesterase (Cartwright blood group) Homo sapiens 48-52 3411326-7 1988 Class I contains molecules that aggregate in the absence of detergent, when mixed with an AChE-depleted Triton X-100 extract from electric organ. Octoxynol 104-116 acetylcholinesterase (Cartwright blood group) Homo sapiens 90-94 3651530-1 1987 Acetylcholinesterase (AChE) from erythrocytes was solubilized by Triton X-100. Octoxynol 65-77 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 3651530-1 1987 Acetylcholinesterase (AChE) from erythrocytes was solubilized by Triton X-100. Octoxynol 65-77 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-26 3651530-2 1987 Size and charge heterogeneity of AChE was investigated by polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (IEF) in polyacrylamide gels in the presence of 0.5% (v/v) Triton X-100. Octoxynol 184-196 acetylcholinesterase (Cartwright blood group) Homo sapiens 33-37 20493122-4 1986 The AChE activities in NaCl/Triton X-100 extracts from the three groups of patients were all more or less the same, and average levels were similar to those evidenced in controls. Octoxynol 28-40 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-8 3221239-1 1988 Human erythrocyte acetylcholinesterase (AChE) solubilized with Triton X-100 and obtained as a complex with micelles containing Triton and membrane phospholipids was incubated with immunoglobulins (Igs) from patients with amyotrophic lateral sclerosis (ALS) and from normal individuals. Octoxynol 63-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 18-38 3221239-1 1988 Human erythrocyte acetylcholinesterase (AChE) solubilized with Triton X-100 and obtained as a complex with micelles containing Triton and membrane phospholipids was incubated with immunoglobulins (Igs) from patients with amyotrophic lateral sclerosis (ALS) and from normal individuals. Octoxynol 63-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 40-44 3221239-9 1988 This enzyme was isolated by affinity chromatography and was washed with a buffer containing Triton X-100 before desorption from the affinity column, a treatment known to remove all phospholipids from erythrocyte AChE. Octoxynol 92-104 acetylcholinesterase (Cartwright blood group) Homo sapiens 212-216 3657387-1 1987 The membrane-bound acetylcholinesterase (AchE) from human peripheral blood lymphocyte gives only one symmetrical peak on sucrose density gradient centrifugation in the presence of Triton X-100 detergent, with the calculated sedimentation coefficient of 6.5 S. However, this dimeric form of AchE was converted to a monomeric 3.8 S form when treated with 2-mercaptoethanol and iodoacetic acid. Octoxynol 180-192 acetylcholinesterase (Cartwright blood group) Homo sapiens 41-45 3657387-4 1987 Polyacrylamide gel electrophoresis indicated a single band with AchE activity in the presence of Triton X-100. Octoxynol 97-109 acetylcholinesterase (Cartwright blood group) Homo sapiens 64-68 7108585-6 1982 Sucrose gradient velocity sedimentation of Triton X-100 extracts of leech ganglia revealed a major peak of AChE activity at 6.5 S and a small peak at 4.3 S. The pattern of activity in the gradient was the same when intact ganglia were pretreated with echothiophate, although the total activity was reduced by 98%. Octoxynol 43-55 acetylcholinesterase (Cartwright blood group) Homo sapiens 107-111 6501251-6 1984 The properties of these acetylcholinesterase preparations obtained by the action of PI-specific phospholipase C and/or Triton X-100 were studied in detail. Octoxynol 119-131 acetylcholinesterase (Cartwright blood group) Homo sapiens 24-44 3986218-7 1985 The total acetylcholinesterase activity was determined after adding Triton X-100 to the assay medium. Octoxynol 68-80 acetylcholinesterase (Cartwright blood group) Homo sapiens 10-30 3980478-8 1985 FBS acetylcholinesterase was characterized as a G4 form of acetylcholinesterase and was distinguished from bovine erythrocyte acetylcholinesterase on the basis of lectin gel binding, [3H] Triton X-100 binding, amino acid composition, number of catalytic subunits/molecule, and hydrodynamic properties. Octoxynol 188-200 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-24 6466662-3 1984 Monomeric and dimeric acetylcholinesterase showed full enzymatic activity in presence of Triton X-100 whereas in the absence of detergent, activity was decreased to approx. Octoxynol 89-101 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-42