PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33809182-7 2021 Moreover, BACH1 increases glucose uptake and lactate secretion through the expression of metabolic enzymes involved such as hexokinase 2 (HK2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for aerobic glycolysis. Lactic Acid 45-52 hexokinase 2 Homo sapiens 124-136 22382780-4 2012 HK2 is the first of a number of enzymes in cancer cells involved in metabolizing the sugar glucose to lactic acid. Lactic Acid 102-113 hexokinase 2 Homo sapiens 0-3 19690031-5 2009 HKII expression in the cells was verified by reverse-transcriptase polymerase chain reaction, Western blot analysis, adenosine triphosphate (ATP) and lactate production, (18)F-FDG uptake measurement, and confocal microscopy. Lactic Acid 150-157 hexokinase 2 Homo sapiens 0-4 33809182-7 2021 Moreover, BACH1 increases glucose uptake and lactate secretion through the expression of metabolic enzymes involved such as hexokinase 2 (HK2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for aerobic glycolysis. Lactic Acid 45-52 hexokinase 2 Homo sapiens 138-141 34973450-0 2022 Upregulated hexokinase 2 expression induces the apoptosis of dopaminergic neurons by promoting lactate production in Parkinson"s disease. Lactic Acid 95-102 hexokinase 2 Homo sapiens 12-24 34973450-11 2022 Inhibition of HK2 expression attenuated the apoptosis of dopaminergic neurons by downregulating lactate production and AMPK/Akt/mTOR pathway in PD. Lactic Acid 96-103 hexokinase 2 Homo sapiens 14-17 34826616-10 2022 However, knockdown of Hexokinase-2 has no obvious effect on the proliferation of SK-HEP-1 cells, although glucose uptake and lactate release are reduced in siHK2-transfected SK-HEP-1 cells, subsequently, we illustrate that two human hepatoma cell lines exhibit glucose metabolism heterogeneity, which causes the different cell proliferation responses to the inhibition of Hexokinase-2. Lactic Acid 125-132 hexokinase 2 Homo sapiens 22-34 34826616-10 2022 However, knockdown of Hexokinase-2 has no obvious effect on the proliferation of SK-HEP-1 cells, although glucose uptake and lactate release are reduced in siHK2-transfected SK-HEP-1 cells, subsequently, we illustrate that two human hepatoma cell lines exhibit glucose metabolism heterogeneity, which causes the different cell proliferation responses to the inhibition of Hexokinase-2. Lactic Acid 125-132 hexokinase 2 Homo sapiens 372-384 34954904-7 2021 CEBPD promoted glucose uptake and lactate production by upregulating SLC2A1 and HK2, leading to mitochondrial fission, increased extracellular acidification rate and decreased oxygen consumption rate to fuel cell growth. Lactic Acid 34-41 hexokinase 2 Homo sapiens 80-83 34236032-6 2021 Down regulation of HK2 significantly inhibited the lactate secretion and lowered the glycolysis baseline in breast cancer cells. Lactic Acid 51-58 hexokinase 2 Homo sapiens 19-22 34737635-9 2021 HK-II siRNA increased the oxygen consumption rate of cells, significantly inhibited lactic acid production and glucose consumption, and significantly suppressed the upregulation of HK-II, pyruvate kinase M2 (PKM2), pyruvate dehydrogenase (PDH), phosphofructokinase platelet (PFKP), lactate dehydrogenase (LD), and citrate synthase (CS) (all, P<0.01). Lactic Acid 84-95 hexokinase 2 Homo sapiens 0-5 34052833-5 2021 ETV4 loss significantly inhibits the expression of HK2, LDHA as well as other glycolytic enzymes, reduces glucose uptake and lactate release in breast cancer cells. Lactic Acid 125-132 hexokinase 2 Homo sapiens 51-54 35563663-4 2022 Herein, we demonstrated that delta-TT inhibits glucose uptake and lactate production in PTEN-deficient LNCaP and PC3 PCa cells, by specifically decreasing HK2 expression. Lactic Acid 66-73 hexokinase 2 Homo sapiens 155-158 33744226-5 2021 The qPCR and Western blotting results showed that Rabeprazole suppressed cell proliferation by inhibition of HK2-mediated glycolysis in BGC823 cells, leading to decrease glucose uptake and lactate production in a dose-dependent way. Lactic Acid 189-196 hexokinase 2 Homo sapiens 109-112 33753739-7 2021 SUMO-defective hexokinase 2 preferably binds to mitochondria and enhances both glucose consumption and lactate production and decreases mitochondrial respiration in parallel. Lactic Acid 103-110 hexokinase 2 Homo sapiens 15-27 33760124-8 2021 A pronounced increase in the OCR, and decreases in the cell proliferation, glucose uptake capacity, lactic acid content, ECAR and HK2 expression were detected in LC cells subjected to GA treatment or HK2-knockdown. Lactic Acid 100-111 hexokinase 2 Homo sapiens 200-203 30952834-5 2019 Furthermore, we showed that B7-H3 increased glucose consumption and lactate production by promoting hexokinase 2 (HK2) expression in CRC cells, and we also found that HK2 was a key mediator of B7-H3-induced CRC chemoresistance. Lactic Acid 68-75 hexokinase 2 Homo sapiens 167-170 33204264-10 2020 Further research showed that upregulated UCA1 effectively increased the rate of glucose uptake, lactate output, and ECAR value, all of which can be attenuate by HK2 interference and 2-DG, whereas knockdown of UCA1 had the opposite effect. Lactic Acid 96-103 hexokinase 2 Homo sapiens 161-164 31628462-8 2020 Furthermore, the expression of hexokinase 2 (HK2) and glucose transporter type 1 (GLUT1) was upregulated, accompanied by elevated glucose, lactic acid release, and activity of lactate dehydrogenase (LDH). Lactic Acid 139-150 hexokinase 2 Homo sapiens 31-43 31628462-8 2020 Furthermore, the expression of hexokinase 2 (HK2) and glucose transporter type 1 (GLUT1) was upregulated, accompanied by elevated glucose, lactic acid release, and activity of lactate dehydrogenase (LDH). Lactic Acid 139-150 hexokinase 2 Homo sapiens 45-48 31628462-8 2020 Furthermore, the expression of hexokinase 2 (HK2) and glucose transporter type 1 (GLUT1) was upregulated, accompanied by elevated glucose, lactic acid release, and activity of lactate dehydrogenase (LDH). Lactic Acid 176-183 hexokinase 2 Homo sapiens 31-43 31628462-8 2020 Furthermore, the expression of hexokinase 2 (HK2) and glucose transporter type 1 (GLUT1) was upregulated, accompanied by elevated glucose, lactic acid release, and activity of lactate dehydrogenase (LDH). Lactic Acid 176-183 hexokinase 2 Homo sapiens 45-48 31270843-5 2019 Knockdown of HK2 also increased O2 consumption while decreasing the extracellular level of lactate and the phosphorylation of p38-mitogen-activated protein kinase (MAPK). Lactic Acid 91-98 hexokinase 2 Homo sapiens 13-16 31428701-4 2019 Lactate production was increased in compressed CAF cells, in a manner dependent on the expression of metabolic genes ENO2, HK2, and PFKFB3. Lactic Acid 0-7 hexokinase 2 Homo sapiens 123-126 31212816-6 2019 Functionally, knockdown of HK2 in ovarian cancer cell lines and ascites-derived tumor cells hindered lactate production, cell migration and invasion, and cell stemness properties, along with reduced FAK/ERK1/2 activation and metastasis- and stemness-related genes. Lactic Acid 101-108 hexokinase 2 Homo sapiens 27-30 31212816-11 2019 In conclusion, HK2, which is regulated by the tumor microenvironment, controls lactate production and contributes to ovarian cancer metastasis and stemness regulation via FAK/ERK1/2 signaling pathway-mediated MMP9/NANOG/SOX9 expression. Lactic Acid 79-86 hexokinase 2 Homo sapiens 15-18 30871777-0 2019 Fubp1 supports the lactate-Akt-mTOR axis through the upregulation of Hk1 and Hk2. Lactic Acid 19-26 hexokinase 2 Homo sapiens 77-80 33160373-7 2020 The nanoparticle complex with FSH peptides modification effectively depleted HK2 expression and facilitated a shift towards oxidative glucose metabolism, with evidence of increased oxygen consumption rates, decreased extracellular acidification rates, and decreased extracellular lactate and glucose consumption in A2780 ovarian cancer cells and cisplatin-resistant A2780CP counterpart cells. Lactic Acid 280-287 hexokinase 2 Homo sapiens 77-80 31524259-9 2019 Consistent with the downregulation of HK2 induced by miR-185, overexpression of HK2 in OS cells significantly attenuated the inhibitory effects of miR-185 on glucose consumption and lactate production, while depletion of miR-185 promoted the glycolysis of OS cells. Lactic Acid 182-189 hexokinase 2 Homo sapiens 80-83 30144070-4 2019 Overexpression of CAV-1 or HK2 in SMMC-7721 and HepG2 HCC cells enhanced glucose and lactate metabolism and increased cell migration and invasion in transwell assays; knocking down CAV-1 or HK2 had the opposite effects. Lactic Acid 85-92 hexokinase 2 Homo sapiens 27-30 30462852-5 2019 Due to the "Warburg effect," most tumor cells exhibit metabolic changes, for example, increased glucose consumption and lactic acid production resulting from mitochondrial-bound overexpressed hexokinase 2. Lactic Acid 120-131 hexokinase 2 Homo sapiens 192-204 30770778-10 2019 Western blotting showed that U251 cell-derived exosomes upregulated the levels of Glut-1, HK-2, and PKM-2, leading to the induction of glucose consumption and generation of lactate and ATP. Lactic Acid 173-180 hexokinase 2 Homo sapiens 90-94 29864622-13 2018 Further western blotting analysis showed that RA could downregulate the expression of glucose transporter-1 and hexokinase-2, leading to the suppression of glucose consumption and generation of lactate and ATP. Lactic Acid 194-201 hexokinase 2 Homo sapiens 112-124 30631356-5 2018 Likewise, the machinery of glycolysis, enzymes such as pyruvate kinase type M2 and hexokinase 2, is particularly active and ultimately leads to both lactate and tumor formation. Lactic Acid 149-156 hexokinase 2 Homo sapiens 83-95 30054337-7 2018 Increased lactate labeling in tumors correlated with c-Myc-driven expression of hexokinase 2, lactate dehydrogenase A, and the monocarboxylate transporters and was accompanied by increased radioresistance. Lactic Acid 10-17 hexokinase 2 Homo sapiens 80-92 29763606-5 2018 Through inhibition of HK2, miR-216a-5p dampens glycolysis by reducing HK activity, glucose uptake, lactate production, ATP generation, extracellular acidification rate (ECAR), and increasing oxygen consumption rate (OCR) in uveal melanoma cells. Lactic Acid 99-106 hexokinase 2 Homo sapiens 22-25 30013360-8 2018 With the suppression of HK-2 activity, the glycolytic level in HCC cells was substantially reduced, which was evidenced by the decrease of glucose consumption and lactate production. Lactic Acid 163-170 hexokinase 2 Homo sapiens 24-28 28915575-0 2017 Hexokinase 2 promotes tumor growth and metastasis by regulating lactate production in pancreatic cancer. Lactic Acid 64-71 hexokinase 2 Homo sapiens 0-12 29440331-9 2018 The results demonstrated that HK2P1, like HK2, stimulated the glucose uptake and lactate production of human endometrial stromal cells. Lactic Acid 81-88 hexokinase 2 Homo sapiens 30-33 29386513-5 2018 Upon HK2 silencing, glucose flux to pyruvate and lactate is inhibited, but TCA fluxes are maintained. Lactic Acid 49-56 hexokinase 2 Homo sapiens 5-8 28704425-6 2017 We also report a decrease in the protein expression levels of HK2, LDHA and CHKA providing likely mechanisms for the depletion of lactate and PC, respectively. Lactic Acid 130-137 hexokinase 2 Homo sapiens 62-65 28445971-8 2017 Transfection of HepG2 and Hep3B cells with HK2 siRNA significantly decreased glucose consumption and lactate production. Lactic Acid 101-108 hexokinase 2 Homo sapiens 43-46 29777095-11 2018 With the suppression of HK-2 expression, glucose consumption in Hep3B and Huh-7 cells decreased by 48.4% and 56.3%, respectively (p<0.01), and the lactate production also was reduced accordingly (39.5% in Hep3B and 48.6% in Huh-7, p<0.01). Lactic Acid 150-157 hexokinase 2 Homo sapiens 24-28 27363977-8 2017 Subsequent in vitro experiments revealed that both HCT8 and HT29 colon carcinoma cells transfected with specific siRNA for HK2 significantly decreased the lactate production, proliferation activity and migration property. Lactic Acid 155-162 hexokinase 2 Homo sapiens 123-126 28025745-11 2017 In vitro experiments further indicated that HK2 was involved in miR-98-mediated suppression of glucose uptake, lactate production, and cell proliferation. Lactic Acid 111-118 hexokinase 2 Homo sapiens 44-47 26831667-6 2016 With the downregulation of hexokinase-2, glucose uptake and lactate production in tumor cells were dramatically declined. Lactic Acid 60-67 hexokinase 2 Homo sapiens 27-39 28915575-6 2017 Transient and stable knockdown of HK2 in primary PDAC cell lines decreased lactate production, anchorage independent growth (AIG) and invasion through a reconstituted matrix. Lactic Acid 75-82 hexokinase 2 Homo sapiens 34-37 28915575-7 2017 Conversely, stable overexpression of HK2 increased lactate production, cell proliferation, AIG and invasion. Lactic Acid 51-58 hexokinase 2 Homo sapiens 37-40 28915575-8 2017 Pharmacologic inhibition of lactate production reduced the HK2-driven increase in invasion while addition of extracellular lactate enhanced invasion, together providing a link between glycolytic activity and metastatic potential. Lactic Acid 28-35 hexokinase 2 Homo sapiens 59-62 26607110-0 2016 miR-4458 suppresses glycolysis and lactate production by directly targeting hexokinase2 in colon cancer cells. Lactic Acid 35-42 hexokinase 2 Homo sapiens 76-87 26607110-4 2016 Using bioinformatic databases, we predicted that hexokinase2 (HK2), a rate-limiting enzyme in the glycolytic pathway, was a target of miR-4458, so the effects of miR-4458 on glycolysis and lactate production was assessed in colon cancer cells. Lactic Acid 189-196 hexokinase 2 Homo sapiens 49-60 26607110-4 2016 Using bioinformatic databases, we predicted that hexokinase2 (HK2), a rate-limiting enzyme in the glycolytic pathway, was a target of miR-4458, so the effects of miR-4458 on glycolysis and lactate production was assessed in colon cancer cells. Lactic Acid 189-196 hexokinase 2 Homo sapiens 62-65 26607110-8 2016 Moreover, knockdown of HK2 by specific RNAi also suppressed proliferation, glycolysis, and lactate production under both normoxic and hypoxic conditions. Lactic Acid 91-98 hexokinase 2 Homo sapiens 23-26 26607110-9 2016 In conclusion, our findings suggested that miR-4458 inhibited the progression of colon cancer cells by inhibition of glycolysis and lactate production via directly targeting HK2 mRNA. Lactic Acid 132-139 hexokinase 2 Homo sapiens 174-177 26116768-6 2015 Silencing of endogenous HK2 resulted in decreased aerobic glycolysis as demonstrated by reduced glucose consumption and lactate production. Lactic Acid 120-127 hexokinase 2 Homo sapiens 24-27 25761777-5 2015 PERK silencing results in decreased glioma cell viability and ATP/lactate production upon low glucose stress, which is mediated by partially blocked AKT activation and subsequent inhibition of Hexokinase II (HK2)"s mitochondria translocation. Lactic Acid 66-73 hexokinase 2 Homo sapiens 208-211 26607087-9 2015 Knockdown of endogenous HK2 by siRNA abolished miR-181 mimics-mediated decrease of glucose uptake and lactate production in CAFs, while transfection with miR-181 mimics suppressed HK2 overexpression-induced enhancement of glucose uptake and lactate production in NFs. Lactic Acid 102-109 hexokinase 2 Homo sapiens 24-27 26607087-9 2015 Knockdown of endogenous HK2 by siRNA abolished miR-181 mimics-mediated decrease of glucose uptake and lactate production in CAFs, while transfection with miR-181 mimics suppressed HK2 overexpression-induced enhancement of glucose uptake and lactate production in NFs. Lactic Acid 241-248 hexokinase 2 Homo sapiens 24-27 26607087-9 2015 Knockdown of endogenous HK2 by siRNA abolished miR-181 mimics-mediated decrease of glucose uptake and lactate production in CAFs, while transfection with miR-181 mimics suppressed HK2 overexpression-induced enhancement of glucose uptake and lactate production in NFs. Lactic Acid 241-248 hexokinase 2 Homo sapiens 180-183 26054020-7 2015 MicroRNA-199a-5p directly targets the 3"-untranslated region of hexokinase 2 (HK2), an enzyme that catalyzes the irreversible first step of glycolysis, thereby suppressing glucose consumption, lactate production, cellular glucose-6-phosphate and adenosine triphosphate levels, cell proliferation, and tumorigenesis of liver cancer cells. Lactic Acid 193-200 hexokinase 2 Homo sapiens 64-76 26054020-7 2015 MicroRNA-199a-5p directly targets the 3"-untranslated region of hexokinase 2 (HK2), an enzyme that catalyzes the irreversible first step of glycolysis, thereby suppressing glucose consumption, lactate production, cellular glucose-6-phosphate and adenosine triphosphate levels, cell proliferation, and tumorigenesis of liver cancer cells. Lactic Acid 193-200 hexokinase 2 Homo sapiens 78-81