PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11904227-2 2002 To understand the mechanism of substrate inhibition, the pH dependence of acetylthiocholine hydrolysis by AChE was studied between pH 5 and 8. Acetylthiocholine 74-91 acetylcholinesterase (Cartwright blood group) Homo sapiens 106-110 12420757-5 2002 The acetylthiocholine detection under enzyme kinetic control was found in the range of 0.01-0.3 U cm(-2) of immobilised AChE. Acetylthiocholine 4-21 acetylcholinesterase (Cartwright blood group) Homo sapiens 120-124 12132693-3 2002 The activity of the pure AChE was also determined using as a substrate different amounts of acetylthiocholine. Acetylthiocholine 92-109 acetylcholinesterase (Cartwright blood group) Homo sapiens 25-29 11163034-2 2001 METHODS: Acetylthiocholine, used as substrate, is hydrolysed by acetylcholinesterase to yield acetate and thiocholine. Acetylthiocholine 9-26 acetylcholinesterase (Cartwright blood group) Homo sapiens 64-84 11275256-1 2000 We have previously described a catalytic monoclonal antibody, raised against acetylcholinesterase (AChE) and capable of hydrolysing acetylthiocholine. Acetylthiocholine 132-149 acetylcholinesterase (Cartwright blood group) Homo sapiens 77-97 11275256-1 2000 We have previously described a catalytic monoclonal antibody, raised against acetylcholinesterase (AChE) and capable of hydrolysing acetylthiocholine. Acetylthiocholine 132-149 acetylcholinesterase (Cartwright blood group) Homo sapiens 99-103 14564607-2 2000 METHODS: The activities of plasma ChE and erythrocyte AChE were determined by the calorimetric method of Ellman et al., using acetylthiocholine as the substrate. Acetylthiocholine 126-143 acetylcholinesterase (Cartwright blood group) Homo sapiens 54-58 10869180-0 2000 Acetylthiocholine binds to asp74 at the peripheral site of human acetylcholinesterase as the first step in the catalytic pathway. Acetylthiocholine 0-17 acetylcholinesterase (Cartwright blood group) Homo sapiens 65-85 10869180-6 2000 In this report, we demonstrate that a key residue in the human AChE peripheral site with which the substrate acetylthiocholine interacts is D74. Acetylthiocholine 109-126 acetylcholinesterase (Cartwright blood group) Homo sapiens 63-67 10869180-8 2000 For human AChE, a K(S) of 1.9+/-0.7 mM obtained by fitting this substrate inhibition curve agreed with a K(S) of 1.3+/-1.0 mM measured directly from acetylthiocholine inhibition of the binding of the neurotoxin fasciculin to the peripheral site. Acetylthiocholine 149-166 acetylcholinesterase (Cartwright blood group) Homo sapiens 10-14 14564607-4 2000 RESULTS: The dissociation constants (K(m)) of plasma ChE and erythrocyte AChE were 5.00 x 10(-5) M and 5.28 x 10(-5) M, respectively, indicating that both enzymes have similar affinity to acetylthiocholine. Acetylthiocholine 188-205 acetylcholinesterase (Cartwright blood group) Homo sapiens 73-77 10340441-3 1999 Considering that AChE only reacts with ACh as substrate and occurs with negligible activities in the serum, the measured individual activity ratio of BChE in the serum (Q(BChE)SE) and the total hydrolysis rate of ACh and BCh in the CSF do allow a precise calculation of the AChE activity in the cerebrospinal fluid. Acetylthiocholine 17-20 acetylcholinesterase (Cartwright blood group) Homo sapiens 274-278 10421442-4 1999 We also report here that acetylthiocholine can bind to the AChE peripheral site with an equilibrium dissociation constant K(S) of about 1 mM. Acetylthiocholine 25-42 acetylcholinesterase (Cartwright blood group) Homo sapiens 59-63 10340441-3 1999 Considering that AChE only reacts with ACh as substrate and occurs with negligible activities in the serum, the measured individual activity ratio of BChE in the serum (Q(BChE)SE) and the total hydrolysis rate of ACh and BCh in the CSF do allow a precise calculation of the AChE activity in the cerebrospinal fluid. Acetylthiocholine 39-42 acetylcholinesterase (Cartwright blood group) Homo sapiens 17-21 9568379-4 1998 The fluorescence intensity of physostigmine-inhibited AChE decreased with increasing the substrate (acetylthiocholine) concentration, thus indicating that physostigmine binding to the active site is essential for the development of fluorescence. Acetylthiocholine 100-117 acetylcholinesterase (Cartwright blood group) Homo sapiens 54-58 9890890-6 1999 Previous reaction schemes for substrate hydrolysis by AChE were extended to include product dissociation steps, and acetylthiocholine hydrolysis rates in the presence of propidium under nonequilibrium conditions were simulated with assigned rate constants in the program SCoP. Acetylthiocholine 116-133 acetylcholinesterase (Cartwright blood group) Homo sapiens 54-58 9192101-1 1997 The activity characteristics of membrane acetylcholinesterase from red blood cells of diabetic patients are very different from those of healthy donors: the limiting enzyme reaction rate is 17.2 +/- 0.8 mumol acetylthiocholine per ml packed cells per min compared with 13.1 +/- 0.8 mumol for control cells. Acetylthiocholine 209-226 acetylcholinesterase (Cartwright blood group) Homo sapiens 41-61 9427520-4 1997 Enhancement of acetylthiocholine hydrolysis also occurred with Torpedo AChE, which has no consensus motif for PKA phosphorylation. Acetylthiocholine 15-32 acetylcholinesterase (Cartwright blood group) Homo sapiens 71-75 8264551-2 1993 AE-2 partially inhibited the rate of hydrolysis of the charged substrate acetylthiocholine by FBS AChE, whereas it increased the rate of hydrolysis of the neutral substrate indophenyl acetate. Acetylthiocholine 73-90 acetylcholinesterase (Cartwright blood group) Homo sapiens 98-102 8951045-3 1996 The kinetic parameter, Km, for the substrate acetylthiocholine was found to be 0.22 mM, and the Kis and Kii for MPDP+ inhibition of AChE were found to be 0.265 and 0.578 mM, respectively. Acetylthiocholine 45-62 acetylcholinesterase (Cartwright blood group) Homo sapiens 132-136 8302294-3 1993 The kinetic parameter, Km for the substrate (acetylthiocholine), was found to be 0.216 mM and Ki for MPP+ for the inactivation of AChE was found to be 0.197 mM. Acetylthiocholine 45-62 acetylcholinesterase (Cartwright blood group) Homo sapiens 130-134 1446827-6 1992 The purified (specific activity of 6000 units per mg protein) homodimer and tetramer enzyme molecules displayed typical AChE biochemical properties: a Km value of 120 microM for acetylthiocholine; a kcat value of 3.9 x 10(5)/min, and selective by AChE-specific inhibitors. Acetylthiocholine 178-195 acetylcholinesterase (Cartwright blood group) Homo sapiens 120-124 1293431-2 1992 Because AChE shows substrate specificity and hydrolyzes acetylthiocholine but not butrylthiocholine, this parasitic enzyme is likely a true acetylcholinesterase. Acetylthiocholine 56-73 acetylcholinesterase (Cartwright blood group) Homo sapiens 8-12 1538717-2 1992 THA causes linear mixed inhibition of AchE hydrolysis of acetylthiocholine, a cationic substrate (KI = 3.8 x 10(-9) M), and linear competitive inhibition of AchE hydrolysis of 7-acetoxy-4-methylcoumarin, an uncharged substrate (KI = 6.8 x 10(-9) M), and N-methyl-7-dimethylcarbamoxyquinolinium, a cationic carbamate (KI = 1.5 x 10(-8) M). Acetylthiocholine 57-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 38-42 1447418-2 1992 In terms of estimated kinetic characteristics of AChE-reaction (KM, Vmax, KI), the pattern of enzyme inhibition by the hindered phenol compounds was found to be of non-competitive or mixed type depending on the inhibitor structure or on the substrate acetylcholine or acetylthiocholine used. Acetylthiocholine 268-285 acetylcholinesterase (Cartwright blood group) Homo sapiens 49-53 1504265-3 1992 2-Substituted-2-hydroxy-4,4-dimethylmorpholiniums (hemicholiniums) inhibit acetylcholinesterase (EC 3.1.1.7)-catalyzed hydrolysis of acetylthiocholine (ATCh). Acetylthiocholine 133-150 acetylcholinesterase (Cartwright blood group) Homo sapiens 75-95 1504265-3 1992 2-Substituted-2-hydroxy-4,4-dimethylmorpholiniums (hemicholiniums) inhibit acetylcholinesterase (EC 3.1.1.7)-catalyzed hydrolysis of acetylthiocholine (ATCh). Acetylthiocholine 152-156 acetylcholinesterase (Cartwright blood group) Homo sapiens 75-95 2263619-5 1990 When ligated to the cDNA and constructed into a transcription vector, this sequence encoded a synthetic mRNA translated in microinjected oocytes into catalytically active AcChoEase with marked preference for acetylthiocholine over butyrylthiocholine as a substrate, susceptibility to inhibition by the AcChoEase inhibitor BW284C51, and resistance to the BtChoEase inhibitor tetraisopropylpyrophosphoramide. Acetylthiocholine 208-225 acetylcholinesterase (Cartwright blood group) Homo sapiens 171-180 1907148-4 1991 Control values for red cell acetylcholinesterase (AChE) activity (mumol/min/mL blood) using acetylthiocholine (1 mM) were higher in the human (4.98) and the rhesus monkey (4.14) than in the marmoset (0.84) and the guinea-pig (0.83). Acetylthiocholine 92-109 acetylcholinesterase (Cartwright blood group) Homo sapiens 28-48 1907148-4 1991 Control values for red cell acetylcholinesterase (AChE) activity (mumol/min/mL blood) using acetylthiocholine (1 mM) were higher in the human (4.98) and the rhesus monkey (4.14) than in the marmoset (0.84) and the guinea-pig (0.83). Acetylthiocholine 92-109 acetylcholinesterase (Cartwright blood group) Homo sapiens 50-54 1849451-5 1991 The recombinant gene product exhibits biochemical traits similar to native "true" acetylcholinesterase as manifested by characteristic substrate inhibition, a Km of 117 microM toward acetylthiocholine, and a high sensitivity to the specific acetylcholinesterase inhibitor BW284C51. Acetylthiocholine 183-200 acetylcholinesterase (Cartwright blood group) Homo sapiens 82-102 1849451-5 1991 The recombinant gene product exhibits biochemical traits similar to native "true" acetylcholinesterase as manifested by characteristic substrate inhibition, a Km of 117 microM toward acetylthiocholine, and a high sensitivity to the specific acetylcholinesterase inhibitor BW284C51. Acetylthiocholine 183-200 acetylcholinesterase (Cartwright blood group) Homo sapiens 241-261 2263619-5 1990 When ligated to the cDNA and constructed into a transcription vector, this sequence encoded a synthetic mRNA translated in microinjected oocytes into catalytically active AcChoEase with marked preference for acetylthiocholine over butyrylthiocholine as a substrate, susceptibility to inhibition by the AcChoEase inhibitor BW284C51, and resistance to the BtChoEase inhibitor tetraisopropylpyrophosphoramide. Acetylthiocholine 208-225 acetylcholinesterase (Cartwright blood group) Homo sapiens 302-311 33032200-4 2020 When acetylcholinesterase (AChE) was present, acetylthiocholine (ATCh) was catalytically hydrolyzed into thiocholine, which reduced MnO2 of PTDNP-MnNFs into Mn2+, subsequently blocking the FRET and enhancing the fluorescence. Acetylthiocholine 46-63 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-25 33588528-4 2021 Obviously, MnNS display an excellent response to thiocholine, deriving from the catalyzing hydrolysis of acetylthiocholine (ATCh) by acetylcholinesterase (AChE), which switches a homogeneous electrochemical OP detection process based on the depressing AChE activity with a limit of detection (LOD) of 0.025 ng mL-1. Acetylthiocholine 105-122 acetylcholinesterase (Cartwright blood group) Homo sapiens 133-153 33588528-4 2021 Obviously, MnNS display an excellent response to thiocholine, deriving from the catalyzing hydrolysis of acetylthiocholine (ATCh) by acetylcholinesterase (AChE), which switches a homogeneous electrochemical OP detection process based on the depressing AChE activity with a limit of detection (LOD) of 0.025 ng mL-1. Acetylthiocholine 105-122 acetylcholinesterase (Cartwright blood group) Homo sapiens 155-159 33588528-4 2021 Obviously, MnNS display an excellent response to thiocholine, deriving from the catalyzing hydrolysis of acetylthiocholine (ATCh) by acetylcholinesterase (AChE), which switches a homogeneous electrochemical OP detection process based on the depressing AChE activity with a limit of detection (LOD) of 0.025 ng mL-1. Acetylthiocholine 105-122 acetylcholinesterase (Cartwright blood group) Homo sapiens 252-256 33032200-4 2020 When acetylcholinesterase (AChE) was present, acetylthiocholine (ATCh) was catalytically hydrolyzed into thiocholine, which reduced MnO2 of PTDNP-MnNFs into Mn2+, subsequently blocking the FRET and enhancing the fluorescence. Acetylthiocholine 46-63 acetylcholinesterase (Cartwright blood group) Homo sapiens 27-31 33032200-4 2020 When acetylcholinesterase (AChE) was present, acetylthiocholine (ATCh) was catalytically hydrolyzed into thiocholine, which reduced MnO2 of PTDNP-MnNFs into Mn2+, subsequently blocking the FRET and enhancing the fluorescence. Acetylthiocholine 65-69 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-25 33032200-4 2020 When acetylcholinesterase (AChE) was present, acetylthiocholine (ATCh) was catalytically hydrolyzed into thiocholine, which reduced MnO2 of PTDNP-MnNFs into Mn2+, subsequently blocking the FRET and enhancing the fluorescence. Acetylthiocholine 65-69 acetylcholinesterase (Cartwright blood group) Homo sapiens 27-31 34862575-3 2021 Acetylcholinesterase (AChE) could catalyze the hydrolysis of acetylthiocholine (ATCh) to produce thiocholine which was further reacted with Ehrman"s reagent and decomposed to form a yellow product 2-nitro-5-thiobenate anion (TNB). Acetylthiocholine 61-78 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 34865979-2 2022 In this assay, the analyte PM inhibited the hydrolysis of acetylthiocholine (ATCh) by AChE, preventing the formation of thiocholine (TCh) that would otherwise react with the AuNPs catalyst and deactivate the catalyst. Acetylthiocholine 58-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 86-90 34865979-2 2022 In this assay, the analyte PM inhibited the hydrolysis of acetylthiocholine (ATCh) by AChE, preventing the formation of thiocholine (TCh) that would otherwise react with the AuNPs catalyst and deactivate the catalyst. Acetylthiocholine 77-81 acetylcholinesterase (Cartwright blood group) Homo sapiens 86-90 34968836-3 2022 In this system, OPs can suppress the activity of acetylcholinesterase (AChE) efficiently, blocking the hydrolysis reaction of acetylthiocholine (ATCh) to generate thiocholine (TCh) by AChE. Acetylthiocholine 126-143 acetylcholinesterase (Cartwright blood group) Homo sapiens 49-69 34968836-3 2022 In this system, OPs can suppress the activity of acetylcholinesterase (AChE) efficiently, blocking the hydrolysis reaction of acetylthiocholine (ATCh) to generate thiocholine (TCh) by AChE. Acetylthiocholine 126-143 acetylcholinesterase (Cartwright blood group) Homo sapiens 71-75 34968836-3 2022 In this system, OPs can suppress the activity of acetylcholinesterase (AChE) efficiently, blocking the hydrolysis reaction of acetylthiocholine (ATCh) to generate thiocholine (TCh) by AChE. Acetylthiocholine 126-143 acetylcholinesterase (Cartwright blood group) Homo sapiens 184-188 34968836-3 2022 In this system, OPs can suppress the activity of acetylcholinesterase (AChE) efficiently, blocking the hydrolysis reaction of acetylthiocholine (ATCh) to generate thiocholine (TCh) by AChE. Acetylthiocholine 145-149 acetylcholinesterase (Cartwright blood group) Homo sapiens 49-69 34968836-3 2022 In this system, OPs can suppress the activity of acetylcholinesterase (AChE) efficiently, blocking the hydrolysis reaction of acetylthiocholine (ATCh) to generate thiocholine (TCh) by AChE. Acetylthiocholine 145-149 acetylcholinesterase (Cartwright blood group) Homo sapiens 71-75 34968836-3 2022 In this system, OPs can suppress the activity of acetylcholinesterase (AChE) efficiently, blocking the hydrolysis reaction of acetylthiocholine (ATCh) to generate thiocholine (TCh) by AChE. Acetylthiocholine 145-149 acetylcholinesterase (Cartwright blood group) Homo sapiens 184-188 34875277-0 2022 Interactions of human acetylcholinesterase with phenyl valerate and acetylthiocholine: Thiocholine as an enhancer of phenyl valerate esterase activity. Acetylthiocholine 68-85 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-42 34862575-3 2021 Acetylcholinesterase (AChE) could catalyze the hydrolysis of acetylthiocholine (ATCh) to produce thiocholine which was further reacted with Ehrman"s reagent and decomposed to form a yellow product 2-nitro-5-thiobenate anion (TNB). Acetylthiocholine 61-78 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-26 34476614-3 2021 Acetylcholinesterase hydrolyzed acetylthiocholine to generate thiocholine that bound with Cu2+ strongly via S-Cu-S bond. Acetylthiocholine 32-49 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 35579472-4 2022 After the addition of acetylcholinesterase (AChE), the substrate acetylthiocholine could be hydrolyzed to thiocholine, which has a stronger binding power with mercury ions than T-Hg2+-T base pairs. Acetylthiocholine 65-82 acetylcholinesterase (Cartwright blood group) Homo sapiens 44-48 35421656-2 2022 Specifically, acetylcholinesterase (AChE) hydrolyzes acetylthiocholine into thiocholine (TCh). Acetylthiocholine 53-70 acetylcholinesterase (Cartwright blood group) Homo sapiens 14-34 35421656-2 2022 Specifically, acetylcholinesterase (AChE) hydrolyzes acetylthiocholine into thiocholine (TCh). Acetylthiocholine 53-70 acetylcholinesterase (Cartwright blood group) Homo sapiens 36-40 34404888-4 2021 This was a prospective study of 57 patients undergoing ambulatory or vascular surgery under GA. Cholinesterase activity was measured before the induction of anesthesia, after 15 min and at the end of surgery by calculating the capacity of serum acetylcholinesterase (AChE) and butyrylcholinesterase to hydrolyze AcetylThioCholine. Acetylthiocholine 312-329 acetylcholinesterase (Cartwright blood group) Homo sapiens 245-265 35166114-5 2022 Interestingly, the 2D CMNSs with peroxidase-like properties exhibited a unique response to thiol compounds and were thus employed as highly efficient catalysts to develop HEC sensors for OPs based on the hydrolysis of acetylthiocholine (ATCh) to form thiocholine catalyzed by acetylcholinesterase (AChE) and the inhibition of AChE activity by OPs. Acetylthiocholine 218-235 acetylcholinesterase (Cartwright blood group) Homo sapiens 276-296 35473871-5 2022 Herein, thiocholine could be produced in hydrolysis reaction of acetylthiocholine catalyzed by the acetylcholinesterase (AChE), of which the catalytic activity could be irreversibly inhibitted by the introduction of organophosphates. Acetylthiocholine 64-81 acetylcholinesterase (Cartwright blood group) Homo sapiens 99-119 35473871-5 2022 Herein, thiocholine could be produced in hydrolysis reaction of acetylthiocholine catalyzed by the acetylcholinesterase (AChE), of which the catalytic activity could be irreversibly inhibitted by the introduction of organophosphates. Acetylthiocholine 64-81 acetylcholinesterase (Cartwright blood group) Homo sapiens 121-125 35166114-5 2022 Interestingly, the 2D CMNSs with peroxidase-like properties exhibited a unique response to thiol compounds and were thus employed as highly efficient catalysts to develop HEC sensors for OPs based on the hydrolysis of acetylthiocholine (ATCh) to form thiocholine catalyzed by acetylcholinesterase (AChE) and the inhibition of AChE activity by OPs. Acetylthiocholine 218-235 acetylcholinesterase (Cartwright blood group) Homo sapiens 298-302 35166114-5 2022 Interestingly, the 2D CMNSs with peroxidase-like properties exhibited a unique response to thiol compounds and were thus employed as highly efficient catalysts to develop HEC sensors for OPs based on the hydrolysis of acetylthiocholine (ATCh) to form thiocholine catalyzed by acetylcholinesterase (AChE) and the inhibition of AChE activity by OPs. Acetylthiocholine 218-235 acetylcholinesterase (Cartwright blood group) Homo sapiens 326-330 3229626-2 1988 Rabbit serum was shown to contain two cholinesterases which hydrolysed acetylthiocholine and butyrylthiocholine and one cholinesterase which hydrolysed only butyrylthiocholine. Acetylthiocholine 71-88 acetylcholinesterase (Cartwright blood group) Homo sapiens 38-52 2906603-5 1988 Because butyrylthiocholine is the preferred substrate for cholinesterase (EC 3.1.1.8) and acetylthiocholine for acetylcholinesterase (EC 3.1.1.7), these results indicate that bambuterol is a remarkably selective and potent inhibitor of cholinesterase. Acetylthiocholine 90-107 acetylcholinesterase (Cartwright blood group) Homo sapiens 112-132 2765560-1 1989 The monoclonal antibody (mAb) AE-2 decreases the rate of hydrolysis of acetylthiocholine (ATC) by fetal bovine serum acetylcholinesterase (acetylcholine acetylhydrolase EC 3.1.1.7) (FBS-AChE) (Doctor, B.P. Acetylthiocholine 71-88 acetylcholinesterase (Cartwright blood group) Homo sapiens 117-137 2765560-1 1989 The monoclonal antibody (mAb) AE-2 decreases the rate of hydrolysis of acetylthiocholine (ATC) by fetal bovine serum acetylcholinesterase (acetylcholine acetylhydrolase EC 3.1.1.7) (FBS-AChE) (Doctor, B.P. Acetylthiocholine 71-88 acetylcholinesterase (Cartwright blood group) Homo sapiens 186-190 2765560-1 1989 The monoclonal antibody (mAb) AE-2 decreases the rate of hydrolysis of acetylthiocholine (ATC) by fetal bovine serum acetylcholinesterase (acetylcholine acetylhydrolase EC 3.1.1.7) (FBS-AChE) (Doctor, B.P. Acetylthiocholine 90-93 acetylcholinesterase (Cartwright blood group) Homo sapiens 117-137 2765560-1 1989 The monoclonal antibody (mAb) AE-2 decreases the rate of hydrolysis of acetylthiocholine (ATC) by fetal bovine serum acetylcholinesterase (acetylcholine acetylhydrolase EC 3.1.1.7) (FBS-AChE) (Doctor, B.P. Acetylthiocholine 90-93 acetylcholinesterase (Cartwright blood group) Homo sapiens 186-190 3229626-6 1988 Using selective inhibitors (iso-OMPA, eserine, BNPP and BW-284C51) it was shown that the hydrolysis of acetylthiocholine and butyrylthiocholine in untreated native serum had properties of acetylcholinesterase (EC 3.1.1.7), butyrylcholinesterase (EC 3.1.1.8) and also some properties of carboxylesterase (EC 3.1.1.1). Acetylthiocholine 103-120 acetylcholinesterase (Cartwright blood group) Homo sapiens 188-208 3580004-2 1987 For this reaction measured by irreversible inhibition of the acetylcholinesterase-catalyzed hydrolysis of acetylthiocholine the activation parameters delta H not equal to = 94 kJ/mole and delta S not equal to (25 degrees C) = -9.4 J/mol X deg were obtained. Acetylthiocholine 106-123 acetylcholinesterase (Cartwright blood group) Homo sapiens 61-81 3980478-6 1985 FBS acetylcholinesterase exhibited typical substrate inhibition, had a Km of 120 microM, and a turnover number of 5300 s-1 with the substrate acetylthiocholine. Acetylthiocholine 142-159 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-24 3729986-5 1986 These three inhibitors compete with TC in the inhibition of enzymatic hydrolysis of acetylthiocholine (ASCh); all three of them affect the noncompetitive component of the inhibition of the hydrolysis of ASCh by TC, which arises from the binding of TC to the peripheral anionic site of AChE, but TEA and C-10 affect also the competitive component of this inhibition, which arises from the binding of TC at the catalytic anionic site. Acetylthiocholine 84-101 acetylcholinesterase (Cartwright blood group) Homo sapiens 285-289 3968080-2 1985 Tetranitromethane inhibits acetylcholinesterase with respect to the hydrolysis of both acetylthiocholine and indophenyl acetate. Acetylthiocholine 87-104 acetylcholinesterase (Cartwright blood group) Homo sapiens 27-47 6704184-2 1984 The reaction of acetylcholinesterase (EC 3.1.1.7; human erythrocytes) with phosphostigmine, haloxon and VX was studied, and the effect of three reversible ligands (TMA, edrophonium, coumarin) and of acetylthiocholine upon the time-dependent and time-independent (reversible) inhibition by the organophosphates was evaluated. Acetylthiocholine 199-216 acetylcholinesterase (Cartwright blood group) Homo sapiens 16-36 6704158-7 1984 True AChE activity (measured in the presence of a maximally effective concentration of tetraisopropylpyrophosphoramide) had a Vmax of 13.4 +/- 0.17 nmoles X min-1 X mg protein)-1 and an apparent Km value of 1 X 10(-4)M acetylthiocholine. Acetylthiocholine 219-236 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-9 6849970-7 1983 (c) Hereditary spherocytosis was singly differentiated by an elevated acetylcholinesterase activity with acetylthiocholine and by a vastly diminished sensitivity to stearic acid, while activity with phenylacetate was equal to control. Acetylthiocholine 105-122 acetylcholinesterase (Cartwright blood group) Homo sapiens 70-90 7108127-7 1982 7, 88-95) procedure for acetylthiocholine as substrate, are described as a rapid screening technique for reversible competitive and noncompetitive inhibitors of acetylcholinesterase activity. Acetylthiocholine 24-41 acetylcholinesterase (Cartwright blood group) Homo sapiens 161-181 6136252-1 1983 The measurement of low levels of cholinesterase or acetylcholinesterase by the Ellman method requires correction for a non-enzymatic increase in absorption at 412 millimicron that is due both to non-enzymatic hydrolysis of the acetylthiocholine substrate and to modification of the colour reagent. Acetylthiocholine 227-244 acetylcholinesterase (Cartwright blood group) Homo sapiens 51-71 748338-2 1978 The enzyme proved to be acetylcholinesterase (AChE) since acetylthiocholine was the preferred substrate, and eserine or BW284C5I inhibited the enzyme activity, while isoOMPA was without effect. Acetylthiocholine 58-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 24-44 6894279-2 1981 Dipalmitoyllecithin- and egg lecithin-acetylcholinesterase complexes exhibit an affinity for acetylthiocholine different from that of the free enzyme. Acetylthiocholine 93-110 acetylcholinesterase (Cartwright blood group) Homo sapiens 38-58 6894279-3 1981 The binding to lecithin apparently abolishes the excess substrate inhibition of acetylcholinesterase; the affinity constants of acetylthiocholine, acetylcholine and acetylcarnitine for the lecithin-bound enzymes are higher than the ones found for the free enzyme. Acetylthiocholine 128-145 acetylcholinesterase (Cartwright blood group) Homo sapiens 80-100 7370307-1 1980 Comparative assays were made in a spectrophotometer and a microcalorimeter for the reaction between acetylcholinesterase (EC 3.1.1.7) and acetylthiocholine. Acetylthiocholine 138-155 acetylcholinesterase (Cartwright blood group) Homo sapiens 100-120 222070-2 1979 The Km value of acetylcholinesterase for acetyl thiocholine was not altered in the reaction and the enzyme proved to be more resistant to heat denaturation. Acetylthiocholine 41-59 acetylcholinesterase (Cartwright blood group) Homo sapiens 16-36 748338-2 1978 The enzyme proved to be acetylcholinesterase (AChE) since acetylthiocholine was the preferred substrate, and eserine or BW284C5I inhibited the enzyme activity, while isoOMPA was without effect. Acetylthiocholine 58-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 46-50 33601648-2 2021 OPs effectively reduce the production of thiocholine in the acetylcholinesterase/acetylthiocholine reaction by inhibiting the activity of acetylcholinesterase. Acetylthiocholine 81-98 acetylcholinesterase (Cartwright blood group) Homo sapiens 60-80 5378376-9 1969 Haloxon also combines with acetylcholinesterase by a non-progressive reaction, producing a complex that is reversible by dilution and by high concentrations of acetylcholine and acetylthiocholine. Acetylthiocholine 178-195 acetylcholinesterase (Cartwright blood group) Homo sapiens 27-47 970243-1 1976 The localization of acetylcholinesterase (AChE) activity in the nuclei of the amygdaloid body of man was studied by Gerebtzoff"s modification of Koelle"s acetylthiocholine method on 10 human brains. Acetylthiocholine 154-171 acetylcholinesterase (Cartwright blood group) Homo sapiens 20-40 970243-1 1976 The localization of acetylcholinesterase (AChE) activity in the nuclei of the amygdaloid body of man was studied by Gerebtzoff"s modification of Koelle"s acetylthiocholine method on 10 human brains. Acetylthiocholine 154-171 acetylcholinesterase (Cartwright blood group) Homo sapiens 42-46 33601648-2 2021 OPs effectively reduce the production of thiocholine in the acetylcholinesterase/acetylthiocholine reaction by inhibiting the activity of acetylcholinesterase. Acetylthiocholine 81-98 acetylcholinesterase (Cartwright blood group) Homo sapiens 138-158 34018740-6 2021 Further, MnNFs-Ru are employed for the homogeneous ECL determination of OPs, where acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine (ATCh) into thiocholine, which in turn decomposes MnNFs of MnNFs-Ru into Mn2+, and OPs inhibit AChE activity. Acetylthiocholine 139-156 acetylcholinesterase (Cartwright blood group) Homo sapiens 83-103 34018740-6 2021 Further, MnNFs-Ru are employed for the homogeneous ECL determination of OPs, where acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine (ATCh) into thiocholine, which in turn decomposes MnNFs of MnNFs-Ru into Mn2+, and OPs inhibit AChE activity. Acetylthiocholine 139-156 acetylcholinesterase (Cartwright blood group) Homo sapiens 105-109 34018740-6 2021 Further, MnNFs-Ru are employed for the homogeneous ECL determination of OPs, where acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine (ATCh) into thiocholine, which in turn decomposes MnNFs of MnNFs-Ru into Mn2+, and OPs inhibit AChE activity. Acetylthiocholine 139-156 acetylcholinesterase (Cartwright blood group) Homo sapiens 252-256 34018740-6 2021 Further, MnNFs-Ru are employed for the homogeneous ECL determination of OPs, where acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine (ATCh) into thiocholine, which in turn decomposes MnNFs of MnNFs-Ru into Mn2+, and OPs inhibit AChE activity. Acetylthiocholine 158-162 acetylcholinesterase (Cartwright blood group) Homo sapiens 83-103 34018740-6 2021 Further, MnNFs-Ru are employed for the homogeneous ECL determination of OPs, where acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine (ATCh) into thiocholine, which in turn decomposes MnNFs of MnNFs-Ru into Mn2+, and OPs inhibit AChE activity. Acetylthiocholine 158-162 acetylcholinesterase (Cartwright blood group) Homo sapiens 105-109 34018740-6 2021 Further, MnNFs-Ru are employed for the homogeneous ECL determination of OPs, where acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine (ATCh) into thiocholine, which in turn decomposes MnNFs of MnNFs-Ru into Mn2+, and OPs inhibit AChE activity. Acetylthiocholine 158-162 acetylcholinesterase (Cartwright blood group) Homo sapiens 252-256 33839958-3 2021 In the presence of AChE, acetylthiocholine (ATCh), a typical AChE substrate, is hydrolyzed to thiocholine (TCh). Acetylthiocholine 25-42 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-23 33961403-4 2021 Interestingly, S-S-AuNCs displayed a unique response to thiol compounds and low pH values and were thus pioneered as a high-efficiency sensor for OPs based on acetylcholinesterase (AChE)-catalyzed hydrolysis of acetylthiocholine into thiocholine and CH3COOH and OP inhibition of AChE activity. Acetylthiocholine 211-228 acetylcholinesterase (Cartwright blood group) Homo sapiens 159-179 33961403-4 2021 Interestingly, S-S-AuNCs displayed a unique response to thiol compounds and low pH values and were thus pioneered as a high-efficiency sensor for OPs based on acetylcholinesterase (AChE)-catalyzed hydrolysis of acetylthiocholine into thiocholine and CH3COOH and OP inhibition of AChE activity. Acetylthiocholine 211-228 acetylcholinesterase (Cartwright blood group) Homo sapiens 181-185 33961403-4 2021 Interestingly, S-S-AuNCs displayed a unique response to thiol compounds and low pH values and were thus pioneered as a high-efficiency sensor for OPs based on acetylcholinesterase (AChE)-catalyzed hydrolysis of acetylthiocholine into thiocholine and CH3COOH and OP inhibition of AChE activity. Acetylthiocholine 211-228 acetylcholinesterase (Cartwright blood group) Homo sapiens 279-283 33839958-3 2021 In the presence of AChE, acetylthiocholine (ATCh), a typical AChE substrate, is hydrolyzed to thiocholine (TCh). Acetylthiocholine 25-42 acetylcholinesterase (Cartwright blood group) Homo sapiens 61-65 33839958-3 2021 In the presence of AChE, acetylthiocholine (ATCh), a typical AChE substrate, is hydrolyzed to thiocholine (TCh). Acetylthiocholine 44-48 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-23 33839958-3 2021 In the presence of AChE, acetylthiocholine (ATCh), a typical AChE substrate, is hydrolyzed to thiocholine (TCh). Acetylthiocholine 44-48 acetylcholinesterase (Cartwright blood group) Homo sapiens 61-65 33592758-7 2021 Thiol-bearing compounds such as thiocholine generated through the hydrolysis of acetylthiocholine by acetylcholinesterase (AChE) interacted with the surface of CdS QDs thus blocking the ECL. Acetylthiocholine 80-97 acetylcholinesterase (Cartwright blood group) Homo sapiens 101-121 34012694-4 2021 Acetylthiocholine (ATCh) could be catalytically hydrolyzed by acetylcholinesterase (AChE) to form thiocholine, which induces aggregation of the AgNPs. Acetylthiocholine 0-17 acetylcholinesterase (Cartwright blood group) Homo sapiens 62-82 34012694-4 2021 Acetylthiocholine (ATCh) could be catalytically hydrolyzed by acetylcholinesterase (AChE) to form thiocholine, which induces aggregation of the AgNPs. Acetylthiocholine 0-17 acetylcholinesterase (Cartwright blood group) Homo sapiens 84-88 33592758-7 2021 Thiol-bearing compounds such as thiocholine generated through the hydrolysis of acetylthiocholine by acetylcholinesterase (AChE) interacted with the surface of CdS QDs thus blocking the ECL. Acetylthiocholine 80-97 acetylcholinesterase (Cartwright blood group) Homo sapiens 123-127 33406172-3 2021 With the presence of thiocholine (TCh), derived from acetylthiocholine (ATCh) hydrolyzed by AChE, the coordination environment of the CDs@Eu/GMP ICPs was interrupted, leading to the collapse of the CDs@Eu/GMP ICP network and the corresponding release of guest CDs into the surrounding environment. Acetylthiocholine 53-70 acetylcholinesterase (Cartwright blood group) Homo sapiens 92-96 33186817-3 2021 This is because that MnO2 NS have oxidized characteristic, and they can react with choline (TCh), which is the product of acetylthiocholine (ATCh) catalyzed by acetylcholinesterase (AChE). Acetylthiocholine 122-139 acetylcholinesterase (Cartwright blood group) Homo sapiens 182-186 33186817-3 2021 This is because that MnO2 NS have oxidized characteristic, and they can react with choline (TCh), which is the product of acetylthiocholine (ATCh) catalyzed by acetylcholinesterase (AChE). Acetylthiocholine 141-145 acetylcholinesterase (Cartwright blood group) Homo sapiens 182-186 33406172-3 2021 With the presence of thiocholine (TCh), derived from acetylthiocholine (ATCh) hydrolyzed by AChE, the coordination environment of the CDs@Eu/GMP ICPs was interrupted, leading to the collapse of the CDs@Eu/GMP ICP network and the corresponding release of guest CDs into the surrounding environment. Acetylthiocholine 72-76 acetylcholinesterase (Cartwright blood group) Homo sapiens 92-96 32892929-3 2020 With the assistance of acetylcholinesterase (AChE), acetylthiocholine (ATCh) is hydrolyzed into thiocholine (TCh) which can effectively etch the ultrathin MnO2 nanosheets, resulting in the dissociation of MnO2-CdS from the photoelectrode. Acetylthiocholine 52-69 acetylcholinesterase (Cartwright blood group) Homo sapiens 23-43 33225325-5 2020 AChE can catalyze the hydrolysis of acetylthiocholine (ATCh) to form thiocholine (TCh), which can induce the fluorescence quenching of AuNCs while having no obvious influence on the fluorescence intensity of FL. Acetylthiocholine 36-53 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 33225325-5 2020 AChE can catalyze the hydrolysis of acetylthiocholine (ATCh) to form thiocholine (TCh), which can induce the fluorescence quenching of AuNCs while having no obvious influence on the fluorescence intensity of FL. Acetylthiocholine 55-59 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 33280706-1 2021 Herein, we propose rapid, precise acetylcholinesterase (AChE) inhibition-based sensing strategy for malathion detection in the presence of Ag-GO and acetylthiocholine (ATCh). Acetylthiocholine 149-166 acetylcholinesterase (Cartwright blood group) Homo sapiens 34-54 33280706-1 2021 Herein, we propose rapid, precise acetylcholinesterase (AChE) inhibition-based sensing strategy for malathion detection in the presence of Ag-GO and acetylthiocholine (ATCh). Acetylthiocholine 149-166 acetylcholinesterase (Cartwright blood group) Homo sapiens 56-60 33280706-1 2021 Herein, we propose rapid, precise acetylcholinesterase (AChE) inhibition-based sensing strategy for malathion detection in the presence of Ag-GO and acetylthiocholine (ATCh). Acetylthiocholine 168-172 acetylcholinesterase (Cartwright blood group) Homo sapiens 34-54 33280706-1 2021 Herein, we propose rapid, precise acetylcholinesterase (AChE) inhibition-based sensing strategy for malathion detection in the presence of Ag-GO and acetylthiocholine (ATCh). Acetylthiocholine 168-172 acetylcholinesterase (Cartwright blood group) Homo sapiens 56-60 33076048-3 2021 When AChE was introduced, acetylthiocholine could be hydrolyzed to generate thiocholine, which efficiently triggered the reduction of MnO2 NSs into Mn2+, resulting in the decrease of fluorescence. Acetylthiocholine 26-43 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-9 33076046-2 2021 The sensing assay is based on the "quenched off" state of bimetallic NC with the addition of Cu2+ ions that can be "switched on" due to generation of thiocholine (TCh), a catalytic product of enzymatic reaction of acetylthiocholine (ATCh) using acetylcholinesterase (AChE) enzyme. Acetylthiocholine 214-231 acetylcholinesterase (Cartwright blood group) Homo sapiens 245-265 33076046-2 2021 The sensing assay is based on the "quenched off" state of bimetallic NC with the addition of Cu2+ ions that can be "switched on" due to generation of thiocholine (TCh), a catalytic product of enzymatic reaction of acetylthiocholine (ATCh) using acetylcholinesterase (AChE) enzyme. Acetylthiocholine 214-231 acetylcholinesterase (Cartwright blood group) Homo sapiens 267-271 33049656-5 2020 We measured blood acetylcholinesterase (AChE) activity using the acetylcholine analog acetylthiocholine. Acetylthiocholine 86-103 acetylcholinesterase (Cartwright blood group) Homo sapiens 18-38 33049656-5 2020 We measured blood acetylcholinesterase (AChE) activity using the acetylcholine analog acetylthiocholine. Acetylthiocholine 86-103 acetylcholinesterase (Cartwright blood group) Homo sapiens 40-44 32805836-4 2020 With the presence of thiocholine (TCh), an enzymatic product hydrolyzed from acetylthiocholine (ATCh) by AChE, the competitive coordination of Tb3+ between GMP and TCh results in the collapse of ICP network and thereby the release of GQDs into the solution, thus, the fluorescence of Tb/GMP turns off and the fluorescence of GQDs turns on. Acetylthiocholine 77-94 acetylcholinesterase (Cartwright blood group) Homo sapiens 105-109 32805836-4 2020 With the presence of thiocholine (TCh), an enzymatic product hydrolyzed from acetylthiocholine (ATCh) by AChE, the competitive coordination of Tb3+ between GMP and TCh results in the collapse of ICP network and thereby the release of GQDs into the solution, thus, the fluorescence of Tb/GMP turns off and the fluorescence of GQDs turns on. Acetylthiocholine 96-100 acetylcholinesterase (Cartwright blood group) Homo sapiens 105-109 32892929-3 2020 With the assistance of acetylcholinesterase (AChE), acetylthiocholine (ATCh) is hydrolyzed into thiocholine (TCh) which can effectively etch the ultrathin MnO2 nanosheets, resulting in the dissociation of MnO2-CdS from the photoelectrode. Acetylthiocholine 52-69 acetylcholinesterase (Cartwright blood group) Homo sapiens 45-49 32892929-3 2020 With the assistance of acetylcholinesterase (AChE), acetylthiocholine (ATCh) is hydrolyzed into thiocholine (TCh) which can effectively etch the ultrathin MnO2 nanosheets, resulting in the dissociation of MnO2-CdS from the photoelectrode. Acetylthiocholine 71-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 23-43 32892929-3 2020 With the assistance of acetylcholinesterase (AChE), acetylthiocholine (ATCh) is hydrolyzed into thiocholine (TCh) which can effectively etch the ultrathin MnO2 nanosheets, resulting in the dissociation of MnO2-CdS from the photoelectrode. Acetylthiocholine 71-75 acetylcholinesterase (Cartwright blood group) Homo sapiens 45-49 32397331-9 2020 We monitored the enzymatic activity of AChE through the SERS spectrum of thiocholine (TC), the end product from acetylthiocholine (ATC). Acetylthiocholine 112-129 acetylcholinesterase (Cartwright blood group) Homo sapiens 39-43 32833082-7 2020 When AChE was present, the substrate acetylthiocholine (ATCh) was hydrolyzed to thiocholine (TCh) that is capable of decomposing MnO2 nanosheets. Acetylthiocholine 37-54 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-9 32833082-7 2020 When AChE was present, the substrate acetylthiocholine (ATCh) was hydrolyzed to thiocholine (TCh) that is capable of decomposing MnO2 nanosheets. Acetylthiocholine 56-60 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-9 32833082-15 2020 When AChE is present, acetylthiocholine iodide (ATCh) is hydrolyzed to thiocholine (TCh) with reducibility for decomposing MnO2 nanosheets. Acetylthiocholine 48-52 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-9 31560517-5 2019 The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. Acetylthiocholine 91-108 acetylcholinesterase (Cartwright blood group) Homo sapiens 31-51 31560517-5 2019 The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. Acetylthiocholine 91-108 acetylcholinesterase (Cartwright blood group) Homo sapiens 53-57 31560517-5 2019 The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. Acetylthiocholine 91-108 acetylcholinesterase (Cartwright blood group) Homo sapiens 310-314 31560517-5 2019 The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. Acetylthiocholine 110-114 acetylcholinesterase (Cartwright blood group) Homo sapiens 31-51 31560517-5 2019 The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. Acetylthiocholine 110-114 acetylcholinesterase (Cartwright blood group) Homo sapiens 53-57 31560517-5 2019 The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. Acetylthiocholine 110-114 acetylcholinesterase (Cartwright blood group) Homo sapiens 310-314 31247192-5 2019 For AChE, two visible substrates were used, acetylthiocholine and 3-(acetamido)-N,N,N-trimethylanilinium. Acetylthiocholine 44-61 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-8 31353389-5 2019 In the presence of acetylcholinesterase (AChE), acetylthiocholine (ATCh) could be hydrolyzed to thiocholine (TCh), which can reduce MnO2 to Mn2+ and trigger the decomposition of MnO2 nanosheets. Acetylthiocholine 48-65 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-39 31353389-5 2019 In the presence of acetylcholinesterase (AChE), acetylthiocholine (ATCh) could be hydrolyzed to thiocholine (TCh), which can reduce MnO2 to Mn2+ and trigger the decomposition of MnO2 nanosheets. Acetylthiocholine 48-65 acetylcholinesterase (Cartwright blood group) Homo sapiens 41-45 31353389-5 2019 In the presence of acetylcholinesterase (AChE), acetylthiocholine (ATCh) could be hydrolyzed to thiocholine (TCh), which can reduce MnO2 to Mn2+ and trigger the decomposition of MnO2 nanosheets. Acetylthiocholine 67-71 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-39 31353389-5 2019 In the presence of acetylcholinesterase (AChE), acetylthiocholine (ATCh) could be hydrolyzed to thiocholine (TCh), which can reduce MnO2 to Mn2+ and trigger the decomposition of MnO2 nanosheets. Acetylthiocholine 67-71 acetylcholinesterase (Cartwright blood group) Homo sapiens 41-45 31020297-4 2019 AChE can hydrolyze acetylthiocholine (ATCh) to form thiocholine (TCh) which contains a thiol group. Acetylthiocholine 19-36 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 31061298-4 2019 With AChE, its substrate ACh will be hydrolyzed into thiocholine and the fluorescence signals exhibit a dramatic decrease at 465 nm, Under optimal conditions, the fluorescent probe shows sensitive responses to AChE in the range of 0.01-0.6 mU/mL. Acetylthiocholine 5-8 acetylcholinesterase (Cartwright blood group) Homo sapiens 210-214 30672607-8 2019 Enzyme stability was also studied, which exhibited that immobilized AChE retained its catalytic activity up to 60 days and retained 80% of the hydrolytic activity even at 37 C. On the basis of the success of immobilized enzyme (covalent) being inhibited by acetylthiocholine, the sensor was administered for the inhibition by monocrotophos and dimethoate that are used widely as pesticides in agricultural. Acetylthiocholine 257-274 acetylcholinesterase (Cartwright blood group) Homo sapiens 68-72 31363918-4 2019 Acetylthiocholine is enzymatically split by AChE to produce thiocholine which triggers the decomposition of Ellmans"s reagent to form a yellow colored product (2-nitro-5-thiobenzoate anion). Acetylthiocholine 0-17 acetylcholinesterase (Cartwright blood group) Homo sapiens 44-48 30876606-9 2019 Thiocholine (TCh), which produced from acetylthiocholine(ATCh) by the hydrolysis of acetylcholinesterase (AChE), can "turn on" the fluorescence sensor. Acetylthiocholine 39-56 acetylcholinesterase (Cartwright blood group) Homo sapiens 84-104 30876606-9 2019 Thiocholine (TCh), which produced from acetylthiocholine(ATCh) by the hydrolysis of acetylcholinesterase (AChE), can "turn on" the fluorescence sensor. Acetylthiocholine 39-56 acetylcholinesterase (Cartwright blood group) Homo sapiens 106-110 31020297-4 2019 AChE can hydrolyze acetylthiocholine (ATCh) to form thiocholine (TCh) which contains a thiol group. Acetylthiocholine 38-42 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 30888170-3 2019 Upon addition of AChE and acetylthiocholine (ATCh), the enzymatic hydrolysate (thiocholine) could seize Cu2+ from UCNPs-Cu2+ mixture, resulting in the quenched FL triggered on. Acetylthiocholine 45-49 acetylcholinesterase (Cartwright blood group) Homo sapiens 17-21 30822441-2 2019 Ellman"s method using Acetylthiocholine (ATCh) is the standard approach for the detection of AChE activity. Acetylthiocholine 22-39 acetylcholinesterase (Cartwright blood group) Homo sapiens 93-97 30822441-2 2019 Ellman"s method using Acetylthiocholine (ATCh) is the standard approach for the detection of AChE activity. Acetylthiocholine 41-45 acetylcholinesterase (Cartwright blood group) Homo sapiens 93-97 30849722-3 2019 The AChE or BChE catalyzed hydrolysis reaction of acetylthiocholine or butyrylthiocholine to generate thiocholine, whose sulfhydryl group strongly captured Cu2+ to inhibit the oxidization of OPD, thus effectively preserving the natural fluorescence emission of CDs. Acetylthiocholine 50-67 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-8 29567179-4 2018 The detection mechanism monitors the inhibition of the activity of acetylcholinesterase (AChE) by the pesticide, in which the production of thiocholine from the hydrolysis of acetylthiocholine (ATCh) catalyzed by AChE is reduced. Acetylthiocholine 175-192 acetylcholinesterase (Cartwright blood group) Homo sapiens 67-87 30938485-4 2019 Taking advantage of their favorable structure and composition, the optimized product exhibits superior electrochemical activity toward thiocholine produced by AChE-catalyzed hydrolysis of acetylthiocholine. Acetylthiocholine 188-205 acetylcholinesterase (Cartwright blood group) Homo sapiens 159-163 30702092-10 2019 The hydrolysis of acetylthiocholine was catalyzed by AChE on the AuNPs, and the metal coordination polymer was then formed which resulted in the aggregation of the perylene probe and the formation of the excimer emission. Acetylthiocholine 18-35 acetylcholinesterase (Cartwright blood group) Homo sapiens 53-57 30201403-2 2018 Acetylthiocholine (ATCh) is the most popular substrate for the detection of AChE activity. Acetylthiocholine 0-17 acetylcholinesterase (Cartwright blood group) Homo sapiens 76-80 30201403-2 2018 Acetylthiocholine (ATCh) is the most popular substrate for the detection of AChE activity. Acetylthiocholine 19-23 acetylcholinesterase (Cartwright blood group) Homo sapiens 76-80 30565170-3 2019 Based on this discovery, a highly sensitive detection of acetylthiocholine (ATCl) was achieved using the acetylcholinesterase (AChE) biosensor. Acetylthiocholine 57-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 105-125 30565170-3 2019 Based on this discovery, a highly sensitive detection of acetylthiocholine (ATCl) was achieved using the acetylcholinesterase (AChE) biosensor. Acetylthiocholine 57-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 127-131 30565170-3 2019 Based on this discovery, a highly sensitive detection of acetylthiocholine (ATCl) was achieved using the acetylcholinesterase (AChE) biosensor. Acetylthiocholine 76-80 acetylcholinesterase (Cartwright blood group) Homo sapiens 105-125 30565170-3 2019 Based on this discovery, a highly sensitive detection of acetylthiocholine (ATCl) was achieved using the acetylcholinesterase (AChE) biosensor. Acetylthiocholine 76-80 acetylcholinesterase (Cartwright blood group) Homo sapiens 127-131 30565170-5 2019 ATCl was catalyzed by acetylcholinesterase (AChE) to produce thiocholine, which served as the coreactant accelerator to improve the ECL signal of Au NC-S2O82- system. Acetylthiocholine 0-4 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-42 30565170-5 2019 ATCl was catalyzed by acetylcholinesterase (AChE) to produce thiocholine, which served as the coreactant accelerator to improve the ECL signal of Au NC-S2O82- system. Acetylthiocholine 0-4 acetylcholinesterase (Cartwright blood group) Homo sapiens 44-48 29567179-4 2018 The detection mechanism monitors the inhibition of the activity of acetylcholinesterase (AChE) by the pesticide, in which the production of thiocholine from the hydrolysis of acetylthiocholine (ATCh) catalyzed by AChE is reduced. Acetylthiocholine 194-198 acetylcholinesterase (Cartwright blood group) Homo sapiens 67-87 29567179-4 2018 The detection mechanism monitors the inhibition of the activity of acetylcholinesterase (AChE) by the pesticide, in which the production of thiocholine from the hydrolysis of acetylthiocholine (ATCh) catalyzed by AChE is reduced. Acetylthiocholine 194-198 acetylcholinesterase (Cartwright blood group) Homo sapiens 89-93 29567179-4 2018 The detection mechanism monitors the inhibition of the activity of acetylcholinesterase (AChE) by the pesticide, in which the production of thiocholine from the hydrolysis of acetylthiocholine (ATCh) catalyzed by AChE is reduced. Acetylthiocholine 194-198 acetylcholinesterase (Cartwright blood group) Homo sapiens 213-217 29567179-4 2018 The detection mechanism monitors the inhibition of the activity of acetylcholinesterase (AChE) by the pesticide, in which the production of thiocholine from the hydrolysis of acetylthiocholine (ATCh) catalyzed by AChE is reduced. Acetylthiocholine 175-192 acetylcholinesterase (Cartwright blood group) Homo sapiens 89-93 29567179-4 2018 The detection mechanism monitors the inhibition of the activity of acetylcholinesterase (AChE) by the pesticide, in which the production of thiocholine from the hydrolysis of acetylthiocholine (ATCh) catalyzed by AChE is reduced. Acetylthiocholine 175-192 acetylcholinesterase (Cartwright blood group) Homo sapiens 213-217 29594716-3 2018 The enzyme acetylcholinesterase (AChE) is known catalyze the hydrolysis of acetylthiocholine to produce thiocholine. Acetylthiocholine 75-92 acetylcholinesterase (Cartwright blood group) Homo sapiens 11-31 29594716-3 2018 The enzyme acetylcholinesterase (AChE) is known catalyze the hydrolysis of acetylthiocholine to produce thiocholine. Acetylthiocholine 75-92 acetylcholinesterase (Cartwright blood group) Homo sapiens 33-37 27611473-3 2017 AChE catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine which reacts with AuNPs by S-Au conjunction and results the aggregation of AuNPs and change in fluorescence of UCNPs. Acetylthiocholine 33-50 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 28494368-2 2017 By utilizing the acetylcholinesterase (AChE) mediated hydrolysis of acetylthiocholine to thiocholine where the activity of AChE is inhibited by the presence of organophosphate pesticides (OPPs), the subsequent thiocholine-induced aggregation of 10OsCO-Au NPs can be monitored by the change in color of the NPs solution and the variation in intensity of the SERS CO signal. Acetylthiocholine 68-85 acetylcholinesterase (Cartwright blood group) Homo sapiens 17-37 28494368-2 2017 By utilizing the acetylcholinesterase (AChE) mediated hydrolysis of acetylthiocholine to thiocholine where the activity of AChE is inhibited by the presence of organophosphate pesticides (OPPs), the subsequent thiocholine-induced aggregation of 10OsCO-Au NPs can be monitored by the change in color of the NPs solution and the variation in intensity of the SERS CO signal. Acetylthiocholine 68-85 acetylcholinesterase (Cartwright blood group) Homo sapiens 39-43 28494368-2 2017 By utilizing the acetylcholinesterase (AChE) mediated hydrolysis of acetylthiocholine to thiocholine where the activity of AChE is inhibited by the presence of organophosphate pesticides (OPPs), the subsequent thiocholine-induced aggregation of 10OsCO-Au NPs can be monitored by the change in color of the NPs solution and the variation in intensity of the SERS CO signal. Acetylthiocholine 68-85 acetylcholinesterase (Cartwright blood group) Homo sapiens 123-127 27611473-3 2017 AChE catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine which reacts with AuNPs by S-Au conjunction and results the aggregation of AuNPs and change in fluorescence of UCNPs. Acetylthiocholine 52-55 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 27208478-4 2016 And the enzyme of AChE was used to catalyze the substrate of acetylthiocholine (ATCh) to produce thiocholine (TCh). Acetylthiocholine 61-78 acetylcholinesterase (Cartwright blood group) Homo sapiens 18-22 27498323-6 2016 The hydrolysis product of acetylthiocholine catalyzed by AChE induced the maleimide ring destruction and activated the fluorescence performance of TPE. Acetylthiocholine 26-43 acetylcholinesterase (Cartwright blood group) Homo sapiens 57-61 27208478-4 2016 And the enzyme of AChE was used to catalyze the substrate of acetylthiocholine (ATCh) to produce thiocholine (TCh). Acetylthiocholine 80-84 acetylcholinesterase (Cartwright blood group) Homo sapiens 18-22 27136042-4 2016 The fluorescence of PhO-dex-GO remarkably increased as AChE catalyzed the hydrolysis of acetylthiocholine (ATCh) to give thiocholine and acetic acid. Acetylthiocholine 88-105 acetylcholinesterase (Cartwright blood group) Homo sapiens 55-59 27136042-4 2016 The fluorescence of PhO-dex-GO remarkably increased as AChE catalyzed the hydrolysis of acetylthiocholine (ATCh) to give thiocholine and acetic acid. Acetylthiocholine 107-111 acetylcholinesterase (Cartwright blood group) Homo sapiens 55-59 26339933-2 2016 Upon the hydrolysis of acetylthiocholine catalyzed by AChE, the product thiocholine stabilizes the in situ formation of CdS QDs in homogenous solution. Acetylthiocholine 23-40 acetylcholinesterase (Cartwright blood group) Homo sapiens 54-58 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 51-68 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-39 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 51-68 acetylcholinesterase (Cartwright blood group) Homo sapiens 81-85 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 70-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-39 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 70-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 41-45 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 70-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 81-85 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 114-118 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-39 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 114-118 acetylcholinesterase (Cartwright blood group) Homo sapiens 41-45 26547618-4 2016 In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. Acetylthiocholine 114-118 acetylcholinesterase (Cartwright blood group) Homo sapiens 81-85 26839920-3 2016 The competitive binding by a thiol group (-SH), produced in the hydrolysis reaction of acetylthiocholine (ACh) chloride with acetylcholinesterase (AChE), removes mercury ions from the base pairs, causing a nuclease-catalyzed digestion, and the subsequent electrochemical response increase due to the weak electrostatic repulsion between the product-mononucleotides and the ITO electrode. Acetylthiocholine 87-106 acetylcholinesterase (Cartwright blood group) Homo sapiens 125-145 26839920-3 2016 The competitive binding by a thiol group (-SH), produced in the hydrolysis reaction of acetylthiocholine (ACh) chloride with acetylcholinesterase (AChE), removes mercury ions from the base pairs, causing a nuclease-catalyzed digestion, and the subsequent electrochemical response increase due to the weak electrostatic repulsion between the product-mononucleotides and the ITO electrode. Acetylthiocholine 87-106 acetylcholinesterase (Cartwright blood group) Homo sapiens 147-151 25569873-2 2015 The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Acetylthiocholine 214-231 acetylcholinesterase (Cartwright blood group) Homo sapiens 152-172 27682399-3 2016 Active acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine into -SH containing thiocholine to replace the NC-dots and trigger the aggregation of AuNPs. Acetylthiocholine 63-80 acetylcholinesterase (Cartwright blood group) Homo sapiens 7-27 27682399-3 2016 Active acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine into -SH containing thiocholine to replace the NC-dots and trigger the aggregation of AuNPs. Acetylthiocholine 63-80 acetylcholinesterase (Cartwright blood group) Homo sapiens 29-33 26141104-2 2015 Subsequently, an AuNCs@11-MUA-Cu(2+) ensemble-based fluorescent chemosensor, which is amenable to convenient, sensitive, selective, turn-on and real-time assay of acetylcholinesterase (AChE), could be developed by using acetylthiocholine (ATCh) as the substrate. Acetylthiocholine 220-237 acetylcholinesterase (Cartwright blood group) Homo sapiens 163-183 26141104-2 2015 Subsequently, an AuNCs@11-MUA-Cu(2+) ensemble-based fluorescent chemosensor, which is amenable to convenient, sensitive, selective, turn-on and real-time assay of acetylcholinesterase (AChE), could be developed by using acetylthiocholine (ATCh) as the substrate. Acetylthiocholine 220-237 acetylcholinesterase (Cartwright blood group) Homo sapiens 185-189 26141104-2 2015 Subsequently, an AuNCs@11-MUA-Cu(2+) ensemble-based fluorescent chemosensor, which is amenable to convenient, sensitive, selective, turn-on and real-time assay of acetylcholinesterase (AChE), could be developed by using acetylthiocholine (ATCh) as the substrate. Acetylthiocholine 239-243 acetylcholinesterase (Cartwright blood group) Homo sapiens 163-183 26141104-2 2015 Subsequently, an AuNCs@11-MUA-Cu(2+) ensemble-based fluorescent chemosensor, which is amenable to convenient, sensitive, selective, turn-on and real-time assay of acetylcholinesterase (AChE), could be developed by using acetylthiocholine (ATCh) as the substrate. Acetylthiocholine 239-243 acetylcholinesterase (Cartwright blood group) Homo sapiens 185-189 25660508-3 2015 Both AChE and BChE can catalyze the hydrolysis of the acetylthiocholine (ATCh) substrate and produce positively-charged thiocholine (TCh). Acetylthiocholine 54-71 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-9 25660508-3 2015 Both AChE and BChE can catalyze the hydrolysis of the acetylthiocholine (ATCh) substrate and produce positively-charged thiocholine (TCh). Acetylthiocholine 73-77 acetylcholinesterase (Cartwright blood group) Homo sapiens 5-9 25569873-2 2015 The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Acetylthiocholine 214-231 acetylcholinesterase (Cartwright blood group) Homo sapiens 174-178 25569873-2 2015 The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Acetylthiocholine 233-236 acetylcholinesterase (Cartwright blood group) Homo sapiens 152-172 25569873-2 2015 The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Acetylthiocholine 233-236 acetylcholinesterase (Cartwright blood group) Homo sapiens 174-178 25560517-5 2015 Using acetylthiocholine (ATCh) as the substrate, this H39GFP/Cu(2+) complex-based sensor was further applied for the turn-on fluorescence detection of acetylcholinesterase (AChE) activity. Acetylthiocholine 6-23 acetylcholinesterase (Cartwright blood group) Homo sapiens 151-171 25560517-5 2015 Using acetylthiocholine (ATCh) as the substrate, this H39GFP/Cu(2+) complex-based sensor was further applied for the turn-on fluorescence detection of acetylcholinesterase (AChE) activity. Acetylthiocholine 6-23 acetylcholinesterase (Cartwright blood group) Homo sapiens 173-177 25560517-5 2015 Using acetylthiocholine (ATCh) as the substrate, this H39GFP/Cu(2+) complex-based sensor was further applied for the turn-on fluorescence detection of acetylcholinesterase (AChE) activity. Acetylthiocholine 25-29 acetylcholinesterase (Cartwright blood group) Homo sapiens 151-171 25560517-5 2015 Using acetylthiocholine (ATCh) as the substrate, this H39GFP/Cu(2+) complex-based sensor was further applied for the turn-on fluorescence detection of acetylcholinesterase (AChE) activity. Acetylthiocholine 25-29 acetylcholinesterase (Cartwright blood group) Homo sapiens 173-177 26165279-2 2015 Thiocholine (TCh) produced in the enzymatic reaction of AChE with acetylthiocholine (ATCh) as a substrate was oxidized on a microelectrode array formed in a main flow channel. Acetylthiocholine 66-83 acetylcholinesterase (Cartwright blood group) Homo sapiens 56-60 25913282-4 2015 The sensing mechanism of this array is based on the irreversible inhibition capability of OPs and carbamates to the activity of acetylcholinesterase (AChE), preventing production of thiocholine and H2O2 from S-acetylthiocholine and acetylcholine and thus resulting in decreased or no color reactions to thiocholine and H2O2 sensitive indicators. Acetylthiocholine 210-227 acetylcholinesterase (Cartwright blood group) Homo sapiens 128-148 25913282-4 2015 The sensing mechanism of this array is based on the irreversible inhibition capability of OPs and carbamates to the activity of acetylcholinesterase (AChE), preventing production of thiocholine and H2O2 from S-acetylthiocholine and acetylcholine and thus resulting in decreased or no color reactions to thiocholine and H2O2 sensitive indicators. Acetylthiocholine 210-227 acetylcholinesterase (Cartwright blood group) Homo sapiens 150-154 26165279-2 2015 Thiocholine (TCh) produced in the enzymatic reaction of AChE with acetylthiocholine (ATCh) as a substrate was oxidized on a microelectrode array formed in a main flow channel. Acetylthiocholine 85-89 acetylcholinesterase (Cartwright blood group) Homo sapiens 56-60 24395570-3 2014 Cholinergic status was determined by measuring the cumulative capacity of serum acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) to hydrolyze the AChE substrate acetylthiocholine. Acetylthiocholine 173-190 acetylcholinesterase (Cartwright blood group) Homo sapiens 80-100 24395570-3 2014 Cholinergic status was determined by measuring the cumulative capacity of serum acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) to hydrolyze the AChE substrate acetylthiocholine. Acetylthiocholine 173-190 acetylcholinesterase (Cartwright blood group) Homo sapiens 102-106 24395570-3 2014 Cholinergic status was determined by measuring the cumulative capacity of serum acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) to hydrolyze the AChE substrate acetylthiocholine. Acetylthiocholine 173-190 acetylcholinesterase (Cartwright blood group) Homo sapiens 158-162 24299064-9 2013 The AChE-catalyzed hydrolysis of acetylthiocholine to the thiol-functionalized thiocholine enabled the probing of the enzymatic activity of AChE through the hemin/G-quadruplex-catalyzed aerobic oxidation of thiocholine to the respective disulfide and the concomitant generation of the fluorescent resorufin product. Acetylthiocholine 33-50 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-8 24225492-4 2014 The principle of this approach is based on the hydrolysis of acetylthiocholine (ATCh) by AChE, which yields the thiol-bearing compound thiocholine (TCh) that at trace concentrations stabilized the in situ generated CdS quantum dots (QDs). Acetylthiocholine 61-78 acetylcholinesterase (Cartwright blood group) Homo sapiens 89-93 24225492-4 2014 The principle of this approach is based on the hydrolysis of acetylthiocholine (ATCh) by AChE, which yields the thiol-bearing compound thiocholine (TCh) that at trace concentrations stabilized the in situ generated CdS quantum dots (QDs). Acetylthiocholine 80-84 acetylcholinesterase (Cartwright blood group) Homo sapiens 89-93 24360998-3 2014 The colorimetric and fluorometric assays were based on the following processes: (1) owing to the hydrolysis of acetylthiocholine in the presence of AChE, the fluorescein-based probe can rapidly induce 1,4-addition of the hydrolysis product thiocholine to alpha,beta-unsaturated ketone in the compound 1, resulting in strong fluorescence and absorption changes; (2) in the presence of the corresponding inhibitor, the fluorescence enhancement or the absorption change would be inhibited in that the formation of thiocholine was hindered. Acetylthiocholine 111-128 acetylcholinesterase (Cartwright blood group) Homo sapiens 148-152 24299064-9 2013 The AChE-catalyzed hydrolysis of acetylthiocholine to the thiol-functionalized thiocholine enabled the probing of the enzymatic activity of AChE through the hemin/G-quadruplex-catalyzed aerobic oxidation of thiocholine to the respective disulfide and the concomitant generation of the fluorescent resorufin product. Acetylthiocholine 33-50 acetylcholinesterase (Cartwright blood group) Homo sapiens 140-144 23777789-5 2013 We were able to demonstrate that this is caused by competition between the MWCNTs and acetylthiocholine for the active sites of AChE. Acetylthiocholine 86-103 acetylcholinesterase (Cartwright blood group) Homo sapiens 128-132 22503679-2 2012 The enzyme AChE catalyzes the hydrolysis of acetylthiocholine to thiocholine, which can be electrochemically oxidized. Acetylthiocholine 44-61 acetylcholinesterase (Cartwright blood group) Homo sapiens 11-15 23597308-2 2013 In this method, the AChE molecules catalyzed the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, which in turn can specifically react with fluorescent squaraine derivative, a specific chemodosimeter for thiol-containing compounds, resulting in fluorescence quenching and offering a low fluorometric background for the further detection of AChE inhibitor. Acetylthiocholine 63-80 acetylcholinesterase (Cartwright blood group) Homo sapiens 20-24 23597308-2 2013 In this method, the AChE molecules catalyzed the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, which in turn can specifically react with fluorescent squaraine derivative, a specific chemodosimeter for thiol-containing compounds, resulting in fluorescence quenching and offering a low fluorometric background for the further detection of AChE inhibitor. Acetylthiocholine 63-80 acetylcholinesterase (Cartwright blood group) Homo sapiens 351-355 23597308-2 2013 In this method, the AChE molecules catalyzed the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, which in turn can specifically react with fluorescent squaraine derivative, a specific chemodosimeter for thiol-containing compounds, resulting in fluorescence quenching and offering a low fluorometric background for the further detection of AChE inhibitor. Acetylthiocholine 82-86 acetylcholinesterase (Cartwright blood group) Homo sapiens 20-24 23597308-2 2013 In this method, the AChE molecules catalyzed the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, which in turn can specifically react with fluorescent squaraine derivative, a specific chemodosimeter for thiol-containing compounds, resulting in fluorescence quenching and offering a low fluorometric background for the further detection of AChE inhibitor. Acetylthiocholine 82-86 acetylcholinesterase (Cartwright blood group) Homo sapiens 351-355 23603132-2 2013 In this assay, AChE catalyzes the hydrolysis of acetylthiocholine (ATCh) to form thiocholine which induces fluorescence quenching of DNA-Cu/AgNCs. Acetylthiocholine 48-65 acetylcholinesterase (Cartwright blood group) Homo sapiens 15-19 23603132-2 2013 In this assay, AChE catalyzes the hydrolysis of acetylthiocholine (ATCh) to form thiocholine which induces fluorescence quenching of DNA-Cu/AgNCs. Acetylthiocholine 67-71 acetylcholinesterase (Cartwright blood group) Homo sapiens 15-19 23047027-0 2013 Hydrolysis of low concentrations of the acetylthiocholine analogs acetyl(homo)thiocholine and acetyl(nor)thiocholine by acetylcholinesterase may be limited by selective gating at the enzyme peripheral site. Acetylthiocholine 40-57 acetylcholinesterase (Cartwright blood group) Homo sapiens 120-140 23379662-3 2013 In the presence of AChE, ATCh was hydrolyzed to thiocholine and acetate. Acetylthiocholine 25-29 acetylcholinesterase (Cartwright blood group) Homo sapiens 19-23 23386352-7 2013 This chapter describes both techniques using the deacetylation of acetyl-lysine residues in model peptides by sirtuin enzymes as well as the hydrolysis of acetylthiocholine by acetylcholinesterase as examples. Acetylthiocholine 155-172 acetylcholinesterase (Cartwright blood group) Homo sapiens 176-196 22099654-2 2011 The assay principle is based on catalytic hydrolysis of acetylthiocholine into thiocholine by acetylcholinesterase, which induces the aggregation of Au nanoparticles and the color change from claret-red to purple or even grey. Acetylthiocholine 56-73 acetylcholinesterase (Cartwright blood group) Homo sapiens 94-114 22148672-2 2012 In this method, AChE mediates the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, and the latter further reduces AuCl(4)(-) to Au NPs without Au nanoseeds. Acetylthiocholine 48-65 acetylcholinesterase (Cartwright blood group) Homo sapiens 16-20 22148672-2 2012 In this method, AChE mediates the hydrolysis of acetylthiocholine (ATCl) to form thiocholine, and the latter further reduces AuCl(4)(-) to Au NPs without Au nanoseeds. Acetylthiocholine 67-71 acetylcholinesterase (Cartwright blood group) Homo sapiens 16-20 22148672-4 2012 On the other hand, the hydrolysis of ATCl is inhibited in the presence of ACh or organophosphate pesticides (OPs, a AChE inhibitor), which will decrease the catalytic growth of Au NPs and, as a result, reduce the orientational response of LCs. Acetylthiocholine 37-41 acetylcholinesterase (Cartwright blood group) Homo sapiens 116-120 21600321-2 2011 AChE hydrolyses acetylthiocholine (ATCh) in thiocoline (TC) and acetic acid (AA). Acetylthiocholine 16-33 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 21600321-2 2011 AChE hydrolyses acetylthiocholine (ATCh) in thiocoline (TC) and acetic acid (AA). Acetylthiocholine 35-39 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-4 21994917-1 2011 An electrochemical platform for acetylcholinesterase (AChE) activity assay and its inhibitors screening is developed based on the Michael addition reaction of thiocholine, the hydrolysis product of acetylthiocholine (AsCh) in the presence of AChE, with the electrogenerated o-quinone of catechol-terminated SAMs on a gold electrode. Acetylthiocholine 198-215 acetylcholinesterase (Cartwright blood group) Homo sapiens 32-52 21994917-1 2011 An electrochemical platform for acetylcholinesterase (AChE) activity assay and its inhibitors screening is developed based on the Michael addition reaction of thiocholine, the hydrolysis product of acetylthiocholine (AsCh) in the presence of AChE, with the electrogenerated o-quinone of catechol-terminated SAMs on a gold electrode. Acetylthiocholine 198-215 acetylcholinesterase (Cartwright blood group) Homo sapiens 54-58 21994917-1 2011 An electrochemical platform for acetylcholinesterase (AChE) activity assay and its inhibitors screening is developed based on the Michael addition reaction of thiocholine, the hydrolysis product of acetylthiocholine (AsCh) in the presence of AChE, with the electrogenerated o-quinone of catechol-terminated SAMs on a gold electrode. Acetylthiocholine 198-215 acetylcholinesterase (Cartwright blood group) Homo sapiens 242-246 21376964-2 2011 Under the catalytic effect of acetylcholinesterase (AChE), acetylthiocholine (ATCh) hydrolysis released thiocholine (TCh) which could react with N-(7-dimethylamino-4-methylcoumarin-3-yl) maleimide (DACM) to produce a blue fluorescence compound. Acetylthiocholine 59-76 acetylcholinesterase (Cartwright blood group) Homo sapiens 30-50 21889639-2 2011 Acetylcholinesterase (AChE) mediated the hydrolysis of acetylthiocholine to produce thiocholine, which interacted with the silver nanoparticles to give a specific SERS spectrum. Acetylthiocholine 55-72 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 21889639-2 2011 Acetylcholinesterase (AChE) mediated the hydrolysis of acetylthiocholine to produce thiocholine, which interacted with the silver nanoparticles to give a specific SERS spectrum. Acetylthiocholine 55-72 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-26 21376964-2 2011 Under the catalytic effect of acetylcholinesterase (AChE), acetylthiocholine (ATCh) hydrolysis released thiocholine (TCh) which could react with N-(7-dimethylamino-4-methylcoumarin-3-yl) maleimide (DACM) to produce a blue fluorescence compound. Acetylthiocholine 59-76 acetylcholinesterase (Cartwright blood group) Homo sapiens 52-56 21376964-2 2011 Under the catalytic effect of acetylcholinesterase (AChE), acetylthiocholine (ATCh) hydrolysis released thiocholine (TCh) which could react with N-(7-dimethylamino-4-methylcoumarin-3-yl) maleimide (DACM) to produce a blue fluorescence compound. Acetylthiocholine 78-82 acetylcholinesterase (Cartwright blood group) Homo sapiens 30-50 21376964-2 2011 Under the catalytic effect of acetylcholinesterase (AChE), acetylthiocholine (ATCh) hydrolysis released thiocholine (TCh) which could react with N-(7-dimethylamino-4-methylcoumarin-3-yl) maleimide (DACM) to produce a blue fluorescence compound. Acetylthiocholine 78-82 acetylcholinesterase (Cartwright blood group) Homo sapiens 52-56 20410609-5 2010 The hydrolysis rate of the novel compounds by human AChE was one order of magnitude lower than that of the traditional substrates, acetylthiocholine and acetyl-beta-methylthiocholine, whereas the hydrolysis rate using human butyrylcholinesterase was two orders of magnitude lower than that of the traditional substrates. Acetylthiocholine 131-148 acetylcholinesterase (Cartwright blood group) Homo sapiens 52-56 20949898-2 2010 The assay is based on a disulfide-thiol interchange reaction between the intramolecularly quenched dimeric dye BODIPY FL l-cystine and thiocholine generated by the AChE-catalyzed hydrolysis of acetylthiocholine (ATCh), which results in a brightly fluorescent monomeric product owing to the cleavage of the disulfide-coupled form of the dye. Acetylthiocholine 193-210 acetylcholinesterase (Cartwright blood group) Homo sapiens 164-168 20949898-2 2010 The assay is based on a disulfide-thiol interchange reaction between the intramolecularly quenched dimeric dye BODIPY FL l-cystine and thiocholine generated by the AChE-catalyzed hydrolysis of acetylthiocholine (ATCh), which results in a brightly fluorescent monomeric product owing to the cleavage of the disulfide-coupled form of the dye. Acetylthiocholine 212-216 acetylcholinesterase (Cartwright blood group) Homo sapiens 164-168 20143889-4 2010 Acetylcholinesterase was used as the labeling enzyme to convert acetylthiocholine to thiocholine. Acetylthiocholine 64-81 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 19472276-2 2009 The progress of the enzymatic reaction of the hydrolysis of acetylthiocholine at pH 8 in the presence of AChE and the inhibitor studied is determined by measuring at 230 nm the peak area of the reaction product thiocholine (TCh). Acetylthiocholine 60-77 acetylcholinesterase (Cartwright blood group) Homo sapiens 105-109 19757435-1 2009 The hydrolysis of acetylcholine and acetylthiocholine as catalyzed by the enzyme acetylcholinesterase was monitored by CE with contactless conductivity detection by determining the acetate produced in the reaction. Acetylthiocholine 36-53 acetylcholinesterase (Cartwright blood group) Homo sapiens 81-101 19238297-4 2009 The PQQ electrode was then utilized as a thiol-specific sensor for the real-time monitoring of thiocholine generated from the hydrolysis of acetylthiocholine (ASCh) by acetylcholinesterase (AChE). Acetylthiocholine 140-157 acetylcholinesterase (Cartwright blood group) Homo sapiens 168-188 19238297-4 2009 The PQQ electrode was then utilized as a thiol-specific sensor for the real-time monitoring of thiocholine generated from the hydrolysis of acetylthiocholine (ASCh) by acetylcholinesterase (AChE). Acetylthiocholine 140-157 acetylcholinesterase (Cartwright blood group) Homo sapiens 190-194 19130375-5 2009 The voltammetric results have shown that the current shifts more anodically as the Au/MBT/PANI/AChE/PVAc biosensor responded to successive acetylthiocholine (ATCh) substrate addition under anaerobic conditions in 0.1 M phosphate buffer, KCl (pH 7.2) solution and aqueous organic solvent solutions. Acetylthiocholine 139-156 acetylcholinesterase (Cartwright blood group) Homo sapiens 95-99 19154124-1 2009 We report herein a new colorimetric assay method for acetylcholinesterase (AChE) activity and its inhibitor screening by making use of the following facts: (1) the aggregation of gold nanoparticles (Au-NPs) results in the red-shift of the plasmon absorption due to interparticle plasmon interactions and (2) AChE can catalyze the hydrolysis of acetylthiocholine into thiocholine which can induce the aggregation of Au-NPs. Acetylthiocholine 344-361 acetylcholinesterase (Cartwright blood group) Homo sapiens 53-73 19154124-1 2009 We report herein a new colorimetric assay method for acetylcholinesterase (AChE) activity and its inhibitor screening by making use of the following facts: (1) the aggregation of gold nanoparticles (Au-NPs) results in the red-shift of the plasmon absorption due to interparticle plasmon interactions and (2) AChE can catalyze the hydrolysis of acetylthiocholine into thiocholine which can induce the aggregation of Au-NPs. Acetylthiocholine 344-361 acetylcholinesterase (Cartwright blood group) Homo sapiens 75-79 19130375-5 2009 The voltammetric results have shown that the current shifts more anodically as the Au/MBT/PANI/AChE/PVAc biosensor responded to successive acetylthiocholine (ATCh) substrate addition under anaerobic conditions in 0.1 M phosphate buffer, KCl (pH 7.2) solution and aqueous organic solvent solutions. Acetylthiocholine 158-162 acetylcholinesterase (Cartwright blood group) Homo sapiens 95-99 18602908-0 2008 Monitoring the reaction of carbachol with acetylcholinesterase by thioflavin T fluorescence and acetylthiocholine hydrolysis. Acetylthiocholine 96-113 acetylcholinesterase (Cartwright blood group) Homo sapiens 42-62 18602908-5 2008 Here we show that the reaction of carbachol (carbamoylcholine) with AChE can be monitored both with acetylthiocholine as a reporter substrate and with thioflavin T as a fluorescent reporter group. Acetylthiocholine 100-117 acetylcholinesterase (Cartwright blood group) Homo sapiens 68-72 17702992-8 2007 These results suggest that the concentration-dependent interactions of chlorpyrifos oxon with acetylcholinesterase resulted from a different mechanism than the concentration-dependent interactions of acetylthiocholine. Acetylthiocholine 200-217 acetylcholinesterase (Cartwright blood group) Homo sapiens 94-114 27873775-2 2008 Cyclic voltammetric experiments performed with the SAM-AchE biosensor in phosphate buffer solutions (pH = 7.2) containing acetylthiocholine confirmed the formation of thiocholine and its electrochemical oxidation at Ep = 0.28 V vs Ag/AgCl. Acetylthiocholine 122-139 acetylcholinesterase (Cartwright blood group) Homo sapiens 55-59 17097792-8 2007 Immobilized human erythrocytes were continuously perfused for real-time measurement of acetylcholinesterase activity by a modified Ellman method using 0.45mM acetylthiocholine. Acetylthiocholine 158-175 acetylcholinesterase (Cartwright blood group) Homo sapiens 87-107 17300836-3 2007 Acetylthiocholine and butyrylthiocholine (identified in mammalian studies as diagnostic substrates for AChE and BChE respectively) were hydrolyzed mainly, but not exclusively, by these enzymes. Acetylthiocholine 0-17 acetylcholinesterase (Cartwright blood group) Homo sapiens 103-107 17454383-2 2007 The voltammetric results have shown that the formal potential shifts anodically as the Au/MBT/PANI/AChE/PVAc thick-film biosensor responded to acetylthiocholine substrate addition under anaerobic conditions in selected organic solvent media containing 2% v/v 0.05 M phosphate buffer, 0.1 M KCl (pH 7.2) solution. Acetylthiocholine 143-160 acetylcholinesterase (Cartwright blood group) Homo sapiens 99-103 15241943-1 2004 The power of chosen carbamates and hydrazinium derivatives (carbazates) to inhibit the hydrolysis of acetylthiocholine by butyrylcholinesterase or acetylcholinesterase was tested. Acetylthiocholine 101-118 acetylcholinesterase (Cartwright blood group) Homo sapiens 147-167 17186224-0 2007 An amperometric acetylthiocholine sensor based on immobilization of acetylcholinesterase on a multiwall carbon nanotube-cross-linked chitosan composite. Acetylthiocholine 16-33 acetylcholinesterase (Cartwright blood group) Homo sapiens 68-88 17186224-1 2007 A simple method has been devised for immobilization of acetylcholinesterase (AChE)--covalent bonding to a multiwall carbon nanotube (MWNT)--cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Acetylthiocholine 237-254 acetylcholinesterase (Cartwright blood group) Homo sapiens 55-75 17186224-1 2007 A simple method has been devised for immobilization of acetylcholinesterase (AChE)--covalent bonding to a multiwall carbon nanotube (MWNT)--cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Acetylthiocholine 237-254 acetylcholinesterase (Cartwright blood group) Homo sapiens 77-81 17186224-1 2007 A simple method has been devised for immobilization of acetylcholinesterase (AChE)--covalent bonding to a multiwall carbon nanotube (MWNT)--cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Acetylthiocholine 256-260 acetylcholinesterase (Cartwright blood group) Homo sapiens 55-75 17186224-1 2007 A simple method has been devised for immobilization of acetylcholinesterase (AChE)--covalent bonding to a multiwall carbon nanotube (MWNT)--cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Acetylthiocholine 256-260 acetylcholinesterase (Cartwright blood group) Homo sapiens 77-81 17186224-4 2007 Because of the inherent conductive properties of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl, with a K(app)(m) value of 132 micromol L(-1), forming thiocholine, which was then oxidized to produce a detectable and rapid response. Acetylthiocholine 105-109 acetylcholinesterase (Cartwright blood group) Homo sapiens 75-79 17186224-4 2007 Because of the inherent conductive properties of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl, with a K(app)(m) value of 132 micromol L(-1), forming thiocholine, which was then oxidized to produce a detectable and rapid response. Acetylthiocholine 162-166 acetylcholinesterase (Cartwright blood group) Homo sapiens 75-79 16240314-4 2006 The immobilized AChE could catalyze the hydrolysis of acetylthiocholine with a K(M)app value of 177 microM to form thiocholine, which was then oxidized to produce detectable signal in a linear range of 1.0-500 microM and fast response. Acetylthiocholine 54-71 acetylcholinesterase (Cartwright blood group) Homo sapiens 16-20 16498728-1 2005 Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. Acetylthiocholine 26-43 acetylcholinesterase (Cartwright blood group) Homo sapiens 111-131 16498728-1 2005 Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. Acetylthiocholine 26-43 acetylcholinesterase (Cartwright blood group) Homo sapiens 133-137 16498728-1 2005 Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. Acetylthiocholine 45-49 acetylcholinesterase (Cartwright blood group) Homo sapiens 111-131 16498728-1 2005 Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. Acetylthiocholine 45-49 acetylcholinesterase (Cartwright blood group) Homo sapiens 133-137 15994894-4 2005 It was found that the encounter process between AChE and acetylthiocholine was promoted in solutions with less structured water. Acetylthiocholine 57-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 48-52 15826103-1 2005 The acetylcholine esterase, AChE, mediated hydrolysis of acetylthiocholine (1) yields a reducing agent thiocholine (2) that stimulates the catalytic enlargement of Au NP seeds in the presence of AuCl(4)(-). Acetylthiocholine 57-74 acetylcholinesterase (Cartwright blood group) Homo sapiens 28-32 12094012-2 2002 It was established that this enzyme activity is acetylcholinesterase by substrate specificity (acetylthiocholine, acetyl-beta-methylthiocholine>propionylthiocholine>butyrylthiocholine), substrate inhibition, and specificity of inhibitors (BW284c51>iso-OMPA). Acetylthiocholine 95-112 acetylcholinesterase (Cartwright blood group) Homo sapiens 48-68 12731886-9 2003 Values of a >1 give rise to a hydrolysis profile called substrate activation, and the AChE site-specific mutant W86F, and to a lesser extent wild-type human AChE itself, showed substrate activation with acetylthiocholine as the substrate. Acetylthiocholine 206-223 acetylcholinesterase (Cartwright blood group) Homo sapiens 89-93 12731886-9 2003 Values of a >1 give rise to a hydrolysis profile called substrate activation, and the AChE site-specific mutant W86F, and to a lesser extent wild-type human AChE itself, showed substrate activation with acetylthiocholine as the substrate. Acetylthiocholine 206-223 acetylcholinesterase (Cartwright blood group) Homo sapiens 160-164