PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3005295-5 1986 Both kinetic and equilibrium measurements demonstrate that the critical concentration of gelsolin-capped ADP-actin filaments (8 microM in 1 mM MgCl2 and 0.2 mM ADP) is the same as for the uncapped filaments, proving that the critical concentration is the same at both ends of the equilibrium polymer in ADP as predicted by theory. Magnesium Chloride 143-148 gelsolin Homo sapiens 89-97 8390984-4 1993 Monomeric ATP-actin in 2 mM MgCl2 and 150 mM KCl slowly destroys the filament-severing activity of gelsolin with kinetics that are first order in actin concentration and with an apparent bimolecular rate constant of 0.021 +/- 0.007 microM-1 s-1. Magnesium Chloride 28-33 gelsolin Homo sapiens 99-107 8390984-5 1993 Coincident with the slow complex formation in MgCl2, the actin bound to the calcium-sensitive actin binding domain of gelsolin hydrolyzes its ATP to ADP. Magnesium Chloride 46-51 gelsolin Homo sapiens 118-126 11016945-6 2000 The sodium channel activity induced by disassembly of submembranous microfilaments with cytochalasin D or gelsolin could be abolished by intact actin added to the bath cytosol-like solution in the presence of 1 mm MgCl(2) to induce actin polymerization. Magnesium Chloride 214-221 gelsolin Homo sapiens 106-114 1323562-8 1992 Gelsolin bound to ATP with Kd = 2.4 x 10(-6) M in a solution containing 2 mM MgCl2, whereas micromolar free Ca2+ concentrations inhibited ATP binding. Magnesium Chloride 77-82 gelsolin Homo sapiens 0-8 2999102-5 1985 By blocking the barbed end of filaments with plasma gelsolin, we determined the critical concentration at the pointed end in 1 mM MgCl2 and 0.2 mM ATP to be 4 microM. Magnesium Chloride 130-135 gelsolin Homo sapiens 52-60 6547850-10 1984 However, when the critical concentration at the pointed end is higher than that at the barbed end (i.e., in 2 mM MgCl2), low molar ratios of brevin sharply increase the monomer concentration to the critical concentration of the pointed end. Magnesium Chloride 113-118 gelsolin Homo sapiens 141-147