PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2268143-2 1990 The lipase is present in a high-molecular-mass fraction (greater than 1.5 x 10(6) dalton by gel filtration), but it can be rapidly extracted from the 100,000 x g supernatant by precipitation with polyethyleneglycol 8000 (4%, w/v) and MgCl2 (40 mM) giving about a 10-fold purification. Magnesium Chloride 234-239 lipase-like Brassica napus 4-10