PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2543460-3 1989 Thus long chain spin labels with the nitroxide group near the terminal methyl of the chain, such as 16-doxylstearic acid, its methyl ester, or a phosphatidylglycerol spin label containing 16-doxylstearic acid (PG-SL), are more motionally restricted and/or ordered in the interdigitated bilayer than in the non-interdigitated bilayer. Hydroxylamine 37-46 spindlin 1 Homo sapiens 16-20 2847589-0 1988 Characterization of protein spin labeling by maleimide: evidence for nitroxide reduction. Hydroxylamine 69-78 spindlin 1 Homo sapiens 28-32 2835102-0 1988 Localizing the nitroxide group of fatty acid and voltage-sensitive spin-labels in phospholipid bilayers. Hydroxylamine 15-24 spindlin 1 Homo sapiens 67-71 207308-3 1978 The spin-labeled enzyme has been modified by urea denaturation, reduction, reduction-carboxymethylation, performic acid oxidation, and digestion with proteolytic enzymes in order to monitor changes in the geometry of the protein by changes in the electron paramagnetic resonance (EPR) spectrum of the nitroxide spin-label probe. Hydroxylamine 301-310 spindlin 1 Homo sapiens 4-8 2985057-0 1985 Spin-labeled erythrocyte membranes: direct identification of nitroxide-conjugated proteins. Hydroxylamine 61-70 spindlin 1 Homo sapiens 0-4 2985057-1 1985 The covalent incorporation of a spin-labeled analog of N-ethyl maleimide into erythrocyte membrane proteins has been monitored by electron paramagnetic resonance spectroscopy and the individually labeled proteins detected by immunoblotting techniques, using an anti-nitroxide antibody, following electrophoretic separation of the membrane components. Hydroxylamine 266-275 spindlin 1 Homo sapiens 32-36 6838898-3 1983 The results indicate that phenylalanine exhibits weak hydrophobic binding to hemoglobin, and that the binding site is probably located within about 7-13 A of the nitroxide free electron of spin-labeled hemoglobin. Hydroxylamine 162-171 spindlin 1 Homo sapiens 189-193 221020-3 1979 The quenching efficiency of fatty acid spin probes is dependent on the position of the nitroxide radical group in the fatty acid chain. Hydroxylamine 87-96 spindlin 1 Homo sapiens 39-43 217413-3 1979 These effects are all shown to be of importance in monitoring the rotational motion of carbonmonoxyhemoglobin which is spin labeled with the tightly bound nitroxide label, 4-maleimido-2,2,6,6-tetramethylpiperidinyl-1-oxy. Hydroxylamine 155-164 spindlin 1 Homo sapiens 119-123 178361-4 1976 The nitroxide spin label was placed either on the beta acyl chain or on the choline group. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 191063-2 1977 A number of spin-labeled acyl derivatives of atractyloside, (m,n)acyl-ATR (general formula: CH3- (CH2)mCX(CH2)nCOO-ATR, where X is an o-azolidine ring containing a nitroxide), have been synthesized. Hydroxylamine 164-173 spindlin 1 Homo sapiens 12-16 191063-3 1977 As shown by electron spin resonance (ESR) spectra of spin-labeled acyl-ATR, the nitroxide placed on the acyl chain interacts with the diterpene residue of the atractyloside moiety when incorporated in liposomes. Hydroxylamine 80-89 spindlin 1 Homo sapiens 21-25 191063-3 1977 As shown by electron spin resonance (ESR) spectra of spin-labeled acyl-ATR, the nitroxide placed on the acyl chain interacts with the diterpene residue of the atractyloside moiety when incorporated in liposomes. Hydroxylamine 80-89 spindlin 1 Homo sapiens 53-57 178361-7 1976 When spin-labeled phosphatidylcholine, having a nitroxide on the beta chain but near the polar head-group, was incorporated into the erythrocyte membrane, ascorbate treatment at 0 degrees C allows selective reduction of the signal coming from the outer layer of the membrane. Hydroxylamine 48-57 spindlin 1 Homo sapiens 5-9 32911450-7 2020 In any sample, heterogeneity is found in H-bond formation between the spin-label nitroxide groups and the solvent molecules. Hydroxylamine 81-90 spindlin 1 Homo sapiens 70-74 4357553-2 1973 Selective incorporation of a nitroxide spin label sensitive to active-center geometry. Hydroxylamine 29-38 spindlin 1 Homo sapiens 39-43 33459015-1 2021 The nitroxide spin label is the most widely used probe for electron paramagnetic resonance (EPR) spectroscopy studies of the structure and function of biomolecules. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 33459015-2 2021 However, the role of surrounding environments in determining the dynamics of nitroxide spin labels in biological complex systems remains to be clarified. Hydroxylamine 77-86 spindlin 1 Homo sapiens 87-91 167802-1 1975 Studies are reported on nitroxide spin-labeled tropomyosin. Hydroxylamine 24-33 spindlin 1 Homo sapiens 34-38 4373731-1 1974 Three classes of lipoidal nitroxide spin probes reversibly perturb erythrocyte membranes at low concentrations (10(-10)-10(-5) M). Hydroxylamine 26-35 spindlin 1 Homo sapiens 36-40 4307874-1 1969 The alpha-amino group of valyl-tRNA has been combined chemically with a nitroxide spin label through an amide linkage. Hydroxylamine 72-81 spindlin 1 Homo sapiens 82-86 33757896-0 2021 Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies. Hydroxylamine 0-9 spindlin 1 Homo sapiens 10-14 33929834-4 2021 This quartet state has spin polarization lifetimes longer than 0.1 ms and is characterized by relatively long coherence times of ~1.8 mus even at 80 K. Rabi oscillation experiments reveal that more than 60 single-qubit logic operations can be performed with this system at 80 K. The large magnitude of the nitroxide 14N hyperfine coupling in the quartet state of PDI-TEMPO is resolved in the transient EPR spectra and leads to a further splitting of the quartet state electron spin sublevels. Hydroxylamine 306-315 spindlin 1 Homo sapiens 23-27 33929834-4 2021 This quartet state has spin polarization lifetimes longer than 0.1 ms and is characterized by relatively long coherence times of ~1.8 mus even at 80 K. Rabi oscillation experiments reveal that more than 60 single-qubit logic operations can be performed with this system at 80 K. The large magnitude of the nitroxide 14N hyperfine coupling in the quartet state of PDI-TEMPO is resolved in the transient EPR spectra and leads to a further splitting of the quartet state electron spin sublevels. Hydroxylamine 306-315 spindlin 1 Homo sapiens 477-481 31251620-1 2019 A model-free approach for simulation of EPR spectra of nitroxide spin probes in liquid-crystalline materials was suggested and used to obtain parameters of molecular orientation and rotational mobility. Hydroxylamine 55-64 spindlin 1 Homo sapiens 65-69 30688300-3 2019 Cyclic hydroxylamine spin probes react selectively with superoxide or other radicals to generate a nitroxide signal that can be quantified by EPR spectroscopy. Hydroxylamine 99-108 spindlin 1 Homo sapiens 21-25 30737560-0 2019 Spin polarization in graphene nanoribbons functionalized with nitroxide. Hydroxylamine 62-71 spindlin 1 Homo sapiens 0-4 30737560-3 2019 Such interacting nitroxide groups induce spin polarization of the edge states of stable graphene nanoribbons. Hydroxylamine 17-26 spindlin 1 Homo sapiens 41-45 30737560-4 2019 Graphical abstract Exchange coupling constants inducing spin polarization in graphene nanoribbons functionalized with nitroxides. Hydroxylamine 118-128 spindlin 1 Homo sapiens 56-60 30768266-0 2019 Comment on "Distinct Populations in Spin-Label EPR Spectra from Nitroxides". Hydroxylamine 64-74 spindlin 1 Homo sapiens 36-40 30500095-0 2019 Semi-Rigid Nitroxide Spin Label for Long-Range EPR Distance Measurements of Lipid Bilayer Embedded beta-Peptides. Hydroxylamine 11-20 spindlin 1 Homo sapiens 21-25 29775060-0 2018 Distinct Populations in Spin-Label EPR Spectra from Nitroxides. Hydroxylamine 52-62 spindlin 1 Homo sapiens 24-28 29577465-3 2018 The light-responsive spin-silenced polymer is synthesized via an Ugi post-polymerization modification incorporating paramagnetic nitroxides and a light cleavable fluorophore moiety. Hydroxylamine 129-139 spindlin 1 Homo sapiens 21-25 30192035-3 2018 The calculations confirm the presence of temperature-dependent exchange interaction within the spin triads formed by the nitroxide-copper(II)-nitroxide units, in line with the changes observed in the effective magnetic moment. Hydroxylamine 121-130 spindlin 1 Homo sapiens 95-99 30192035-4 2018 Moreover, they quantify the interchain interaction mediated by the terminal nitroxide group of two spin triads in neighboring polymer chains. Hydroxylamine 76-85 spindlin 1 Homo sapiens 99-103 30686862-3 2018 Here we present a method that allows for spin-labeling of protein nucleotide binding sites by adenosine diphosphate (ADP) modified with a nitroxide moiety on the beta-phosphate (ADP-beta-S-SL). Hydroxylamine 138-147 spindlin 1 Homo sapiens 41-45 30222354-2 2018 Paramagnetic metal ions, such as Cu2+, are used as spin probes because they can report on metalloprotein features and can be spectroscopically distinguished from traditional nitroxide (NO)-based labels. Hydroxylamine 174-183 spindlin 1 Homo sapiens 51-55 29775060-1 2018 Two-component nitroxide spin-label electron paramagnetic presonance (EPR) spectra are important to the analysis of lipid-protein interactions, phase separation in lipid membranes, and conformational changes in proteins. Hydroxylamine 14-23 spindlin 1 Homo sapiens 24-28 29451325-0 2018 Purine-Derived Nitroxides for Noncovalent Spin-Labeling of Abasic Sites in Duplex Nucleic Acids. Hydroxylamine 15-25 spindlin 1 Homo sapiens 42-46 29037084-10 2018 FUTURE DIRECTIONS: Additional studies to advance hydroxylamine spin probes from the "basic science" to biomedical applications are needed and could lead to better understanding of pathological conditions associated with oxidative stress. Hydroxylamine 49-62 spindlin 1 Homo sapiens 63-67 29369431-5 2018 To render a particular target molecule accessible for PELDOR, it can be engineered to contain only one or two surface-exposed cysteine residues, which can be efficiently spin-labelled using thiol-reactive nitroxide compounds. Hydroxylamine 205-214 spindlin 1 Homo sapiens 170-174 29614227-2 2018 This ESEEM method detects dipolar couplings between 2H-labeled nuclei on the side chains of an amino acid (Leu or Val) and a strategically placed nitroxide spin-label in the proximity up to 8 A. ESEEM spectra patterns for different samples correlate directly to the periodic structural feature of different secondary structures. Hydroxylamine 146-155 spindlin 1 Homo sapiens 156-160 29485736-1 2018 EPR studies on RNA are complicated by three major obstacles related to the chemical nature of nitroxide spin labels: Decomposition while oligonucleotides are chemically synthesized, further decay during enzymatic strand ligation, and undetected changes in conformational equilibria due to the steric demand of the label. Hydroxylamine 94-103 spindlin 1 Homo sapiens 104-108 29227566-0 2018 A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies. Hydroxylamine 15-24 spindlin 1 Homo sapiens 25-29 29227566-3 2018 Until now, the use of nitroxide-based spin labels for in-cell studies has represented a major hurdle because of their short persistence in the cellular context. Hydroxylamine 22-31 spindlin 1 Homo sapiens 38-42 28944812-4 2017 The thermochromism of these complexes evident upon thermal spin state switching is mainly caused by a spectral shift of the absorption bands of the nitroxides. Hydroxylamine 148-158 spindlin 1 Homo sapiens 59-63 28877484-2 2017 We compare the computationally generated structural ensembles of the IDP amyloid-beta42 (Abeta42) to an alternative sequence in which a nitroxide spin label attached to cysteine has been introduced at its N-terminus. Hydroxylamine 136-145 spindlin 1 Homo sapiens 146-150 27189101-1 2016 We report the synthesis and characterisation of the nitroxide spin-labelled photoactivatable Pt(iv) prodrug trans,trans,trans-[Pt(N3)2(OH)(OCOCH2CH2CONH-TEMPO)(Py)2] (Pt-TEMPO, where TEMPO = 2,2,6,6-tetramethylpiperidine 1-oxyl). Hydroxylamine 52-61 spindlin 1 Homo sapiens 62-66 27342129-0 2017 Nitroxide Spin-Labelling and Its Role in Elucidating Cuproprotein Structure and Function. Hydroxylamine 0-9 spindlin 1 Homo sapiens 10-14 27342129-3 2017 When spin-labels such as aminoxyl radicals (commonly referred to as nitroxides) are introduced, then EPR becomes a powerful technique to monitor not only the coordination environment, but also to obtain structural information that is often not readily available from other techniques. Hydroxylamine 68-78 spindlin 1 Homo sapiens 5-9 27342129-5 2017 The theory and practice of EPR can be daunting to the non-expert; therefore, in this mini review, we explore how nitroxide spin-labelling can be used to help the inorganic biochemist gain greater understanding of cuproprotein structure and function in vitro and how EPR imaging may help improve understanding of copper homoeostasis in vivo. Hydroxylamine 113-122 spindlin 1 Homo sapiens 123-127 28570782-2 2017 Electron paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labeling strategies are needed. Hydroxylamine 72-82 spindlin 1 Homo sapiens 54-58 28339206-4 2017 In this study, 2H-labeled d10-leucine (Leu) was substituted at a specific Leu residue (i) and a nitroxide spin label was positioned 2, 3, and 4 residues away (denoted i+2 to i+4) on an amphipathic model peptide, LRL8. Hydroxylamine 96-105 spindlin 1 Homo sapiens 106-110 27998079-0 2016 Designing Molecular Probes To Prolong Intracellular Retention: Application to Nitroxide Spin Probes. Hydroxylamine 78-87 spindlin 1 Homo sapiens 88-92 24452480-0 2014 Diversification of EPR signatures in Site Directed Spin Labeling using a beta-phosphorylated nitroxide. Hydroxylamine 93-102 spindlin 1 Homo sapiens 51-55 26477246-0 2015 Saturation Recovery EPR and Nitroxide Spin Labeling for Exploring Structure and Dynamics in Proteins. Hydroxylamine 28-37 spindlin 1 Homo sapiens 38-42 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 255-264 spindlin 1 Homo sapiens 20-24 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 255-264 spindlin 1 Homo sapiens 60-64 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 255-264 spindlin 1 Homo sapiens 60-64 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 375-384 spindlin 1 Homo sapiens 20-24 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 375-384 spindlin 1 Homo sapiens 60-64 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 375-384 spindlin 1 Homo sapiens 60-64 26490692-2 2015 Whether the magnetic interaction occurs predominantly via Heisenberg spin exchange (Ni) or by the dipole-dipole (Gd) mechanism, it is essential for the paramagnetic ion to get into close proximity to the nitroxide moiety for efficient RE. Hydroxylamine 204-213 spindlin 1 Homo sapiens 69-73 26441482-3 2015 This ensures high oxygen concentration and oxygen diffusion at the location of the nitroxide as well as isolation of the nitroxide moiety from cellular reductants and paramagnetic ions that might interfere with spin-label oximetry measurements. Hydroxylamine 121-130 spindlin 1 Homo sapiens 211-215 24452480-2 2014 Conventional spin labels are based on nitroxide derivatives leading to classical 3-line spectra whose spectral shapes are indicative of the environment of the labels and thus constitute good reporters of structural modifications. Hydroxylamine 38-47 spindlin 1 Homo sapiens 13-17 24452480-4 2014 To overcome the limitation due to the weak diversity of nitroxide label EPR spectral shapes, we designed a new spin label based on a beta-phosphorylated nitroxide giving 6-line spectra. Hydroxylamine 56-65 spindlin 1 Homo sapiens 111-115 24452480-4 2014 To overcome the limitation due to the weak diversity of nitroxide label EPR spectral shapes, we designed a new spin label based on a beta-phosphorylated nitroxide giving 6-line spectra. Hydroxylamine 153-162 spindlin 1 Homo sapiens 111-115 23731861-0 2013 Conformational dynamics and distribution of nitroxide spin labels. Hydroxylamine 44-53 spindlin 1 Homo sapiens 54-58 26097251-2 2013 In an attempt to observe dynamic nuclear polarization (DNP) in an MRFM experiment, which could possibly further improve its sensitivity towards a single proton spin, a film of perdeuterated polystyrene doped with a nitroxide electron-spin probe was prepared. Hydroxylamine 215-224 spindlin 1 Homo sapiens 160-164 26097251-2 2013 In an attempt to observe dynamic nuclear polarization (DNP) in an MRFM experiment, which could possibly further improve its sensitivity towards a single proton spin, a film of perdeuterated polystyrene doped with a nitroxide electron-spin probe was prepared. Hydroxylamine 215-224 spindlin 1 Homo sapiens 234-238 26097251-4 2013 In addition to observing the expected prompt change in cantilever frequency due to saturation of the nitroxide"s electron-spin magnetization, we observed a persistent cantilever frequency change. Hydroxylamine 101-110 spindlin 1 Homo sapiens 122-126 23320703-1 2013 For nitroxide radicals in solution one can identify three frequency regimes in which (1)H spin-lattice relaxation rate of solvent molecules depend linearly on square root of the (1)H resonance frequency. Hydroxylamine 4-13 spindlin 1 Homo sapiens 90-94 23556735-0 2013 1H relaxation dispersion in solutions of nitroxide radicals: influence of electron spin relaxation. Hydroxylamine 41-50 spindlin 1 Homo sapiens 83-87 26605629-4 2012 The results from molecular dynamics simulations suggest that the oligothiophene conjugate part of the spin label interacts with hydrophobic residues of the amyloid protein through hydrophobic attraction and that both the N-O bond length and the N-O out-of-plane tilt angle in the nitroxide group are slightly diminished after complexation with the protein. Hydroxylamine 280-289 spindlin 1 Homo sapiens 102-106 26605629-5 2012 The translational and rotational motions of the protein-bound spin label are considerably slowed compared to those of the free spin label in aqueous solution, but interestingly, hydrogen bonds formed between the nitroxide oxygen group and the surrounding water molecules are hardly affected by the presence of the amyloid protein. Hydroxylamine 212-221 spindlin 1 Homo sapiens 62-66 26605629-6 2012 First-principles calculations suggest that EPR spin Hamiltonian parameters including the nitroxide nitrogen hyperfine coupling tensor A(N) and electronic g tensor suffer noticeable changes upon complexation with the protein. Hydroxylamine 89-98 spindlin 1 Homo sapiens 47-51 21541435-1 2011 1,3-Alternate calix[4]arene with para-phenylene spacers connecting nitroxide monoradicals and high-spin (S = 1) diradicals provides tetraradical and octaradical scaffolds that possess conformations with slow electron spin relaxation rates (1/T(1)). Hydroxylamine 67-76 spindlin 1 Homo sapiens 217-221 26592886-1 2012 Encapsulation of a nitroxide spin label into a host cavity can prolong the lifetime of the spin label in biological tissues and other environments. Hydroxylamine 19-28 spindlin 1 Homo sapiens 29-33 26592886-1 2012 Encapsulation of a nitroxide spin label into a host cavity can prolong the lifetime of the spin label in biological tissues and other environments. Hydroxylamine 19-28 spindlin 1 Homo sapiens 91-95 22917445-0 2012 Synthesis of 2,5-bis(spirocyclohexane)-substituted nitroxides of pyrroline and pyrrolidine series, including thiol-specific spin label: an analogue of MTSSL with long relaxation time. Hydroxylamine 51-61 spindlin 1 Homo sapiens 124-128 22917445-1 2012 The nitroxides of 7-azadispiro[5.1.5.2]pentadecane and 7-azadispiro[5.1.5.2]pentadeca-14-ene series have been prepared, including thiol-specific methane thiosulfonate spin label for site-directed spin labeling. Hydroxylamine 4-14 spindlin 1 Homo sapiens 167-171 22917445-1 2012 The nitroxides of 7-azadispiro[5.1.5.2]pentadecane and 7-azadispiro[5.1.5.2]pentadeca-14-ene series have been prepared, including thiol-specific methane thiosulfonate spin label for site-directed spin labeling. Hydroxylamine 4-14 spindlin 1 Homo sapiens 196-200 22917445-3 2012 The obtained temperature dependencies of electron spin relaxation parameters demonstrate that new nitroxides may be suitable for PELDOR distance measurements at 80-120 K. Moreover, the new nitroxides demonstrated much higher stability toward reduction by ascorbate than spirocyclohexane-substituted nitroxides of piperidine series and showed 1.3-3.14 times lower reduction rates compared to corresponding 2,2,5,5-tetramethyl nitroxides. Hydroxylamine 98-108 spindlin 1 Homo sapiens 50-54 22917445-3 2012 The obtained temperature dependencies of electron spin relaxation parameters demonstrate that new nitroxides may be suitable for PELDOR distance measurements at 80-120 K. Moreover, the new nitroxides demonstrated much higher stability toward reduction by ascorbate than spirocyclohexane-substituted nitroxides of piperidine series and showed 1.3-3.14 times lower reduction rates compared to corresponding 2,2,5,5-tetramethyl nitroxides. Hydroxylamine 189-199 spindlin 1 Homo sapiens 50-54 20715877-0 2010 Magnetic properties of nitroxide spin probes: reliable account of molecular motions and nonspecific solvent effects by time-dependent and time-independent approaches. Hydroxylamine 23-32 spindlin 1 Homo sapiens 33-37 20715877-1 2010 Application of a new integrated computational approach for two widely used nitroxide spin probes allows to show unequivocally that proper account of stereoelectronic, environmental, and dynamical effects leads to magnetic properties in quantitative agreement with experimental results without the need of any empirical parameter. Hydroxylamine 75-84 spindlin 1 Homo sapiens 85-89 19290661-2 2009 One of the most powerful approaches to measure local water dynamics within 5 A distances is to utilize the modulation of the nuclear spin relaxation rate of water protons through their time-dependent dipolar coupling to paramagnetic probes, here nitroxide spin labels. Hydroxylamine 246-255 spindlin 1 Homo sapiens 133-137 19568955-4 2009 The relation of these observables to the electronic structure is sketched using the nitroxide group of spin labels as a simple example. Hydroxylamine 84-93 spindlin 1 Homo sapiens 103-107 19960064-3 2010 Calibrations are given, in terms of the Block-Walker reaction field and local proton donor concentration, for the nitroxides that are commonly used in spin labeling of lipids and proteins. Hydroxylamine 114-124 spindlin 1 Homo sapiens 151-155 19290661-2 2009 One of the most powerful approaches to measure local water dynamics within 5 A distances is to utilize the modulation of the nuclear spin relaxation rate of water protons through their time-dependent dipolar coupling to paramagnetic probes, here nitroxide spin labels. Hydroxylamine 246-255 spindlin 1 Homo sapiens 256-260 17929861-2 2007 In this paper, we use large-scale molecular dynamics simulations to investigate the position and behavior of nitroxide spin labels attached to stearic acid molecules in dipalmitoylphosphatidylcholine (DPPC) bilayers. Hydroxylamine 109-118 spindlin 1 Homo sapiens 119-123 18447510-0 2008 Simulating electron spin resonance spectra of nitroxide spin labels from molecular dynamics and stochastic trajectories. Hydroxylamine 46-55 spindlin 1 Homo sapiens 20-24 18447510-0 2008 Simulating electron spin resonance spectra of nitroxide spin labels from molecular dynamics and stochastic trajectories. Hydroxylamine 46-55 spindlin 1 Homo sapiens 56-60 18447510-1 2008 Simulating electron spin resonance spectra of nitroxide spin labels from motional models is necessary for the quantitative analysis of experimental spectra. Hydroxylamine 46-55 spindlin 1 Homo sapiens 20-24 18447510-1 2008 Simulating electron spin resonance spectra of nitroxide spin labels from motional models is necessary for the quantitative analysis of experimental spectra. Hydroxylamine 46-55 spindlin 1 Homo sapiens 56-60 26620180-2 2008 The technique requires attachment of nitroxide spin labels to the nucleotides, which may possibly perturb its conformation. Hydroxylamine 37-46 spindlin 1 Homo sapiens 47-51 26620180-3 2008 To study to what extent nitroxide spin labels may affect RNA structure, all-atom molecular dynamics simulations in explicit solvent are performed for six double-labeled RNA duplexes. Hydroxylamine 24-33 spindlin 1 Homo sapiens 34-38 26620180-4 2008 A new parametrization of the force field for the nitroxide spin label is developed, which leads to intramolecular distances that are in good agreement with experimental results. Hydroxylamine 49-58 spindlin 1 Homo sapiens 59-63 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-1 2008 The synthesis and characterization by SQUID (superconducting quantum interference device) magnetometry of one of the high-spin diradicals, in which nitroxides are conformationally constrained to be coplanar with the m-phenylene unit, is reported. Hydroxylamine 148-158 spindlin 1 Homo sapiens 122-126 12729929-3 2003 The nitroxide spin probe 3beta-doxyl-5alpha-cholestane (cholestane or CLS) was inserted into the bilayer discs to demonstrate the effects of macroscopic bilayer alignment through the measurement of orientational dependent hyperfine splittings. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 15326023-2 2004 As a reporter group, the nitroxide spin-label was attached at the C-terminus yielding the spin-labeled product (GAsl). Hydroxylamine 25-34 spindlin 1 Homo sapiens 35-39 15326023-2 2004 As a reporter group, the nitroxide spin-label was attached at the C-terminus yielding the spin-labeled product (GAsl). Hydroxylamine 25-34 spindlin 1 Homo sapiens 90-94 14750677-1 2004 A series of Au nanoparticles functionalised with nitroxide spin labels has been prepared and studied by EPR spectroscopy. Hydroxylamine 49-58 spindlin 1 Homo sapiens 59-63 14750677-4 2004 The samples with higher coverage of the spin label show an increasing contribution of the exchange interaction between nitroxides adsorbed in a close proximity to each other on the same nanoparticle. Hydroxylamine 119-129 spindlin 1 Homo sapiens 40-44 16620092-0 2006 Synthesis and structure of a nucleoside with pi-conjugated nitroxide spin label forming a one-dimensional ferromagnetic chain. Hydroxylamine 59-68 spindlin 1 Homo sapiens 69-73 14616032-6 2003 The calculations show that the protein environment sterically limits the area of the possible angular reorientations for the NO reporter group of the nitroxide (within the spin label), and this, in turn, affects the shape of the EPR spectrum. Hydroxylamine 150-159 spindlin 1 Homo sapiens 172-176 11604544-1 2001 The binding of a nitroxide spin-labeled analog of N-acetyllactosamine to galectin-3, a mammalian lectin of 26 kD size, is studied to map the binding sites of this small oligosaccharide on the protein surface. Hydroxylamine 17-26 spindlin 1 Homo sapiens 27-31 11921394-2 2002 It is shown here that organic nitroxide radicals ("spin labels") can be used to boost the sensitivity of NMR spectroscopic screening in drug discovery research. Hydroxylamine 30-39 spindlin 1 Homo sapiens 51-55 11458375-0 2001 Spin-Labeled Dendrimers in EPR Imaging with Low Molecular Weight Nitroxides. Hydroxylamine 65-75 spindlin 1 Homo sapiens 0-4 9200687-2 1997 Spin-labeled biotin-PEs were prepared with the nitroxide group at position C-5, C-8, C-10, C-12, or C-14 of the sn-2 chain and were incorporated at 1 mol % in lipid bilayer membranes of dimyristoylphosphatidylcholine. Hydroxylamine 47-56 spindlin 1 Homo sapiens 0-4 10423365-0 1999 Nitroxide side-chain dynamics in a spin-labeled helix-forming peptide revealed by high-frequency (139.5-GHz) EPR spectroscopy. Hydroxylamine 0-9 spindlin 1 Homo sapiens 35-39 10423365-1 1999 High-frequency electron paramagnetic resonance (EPR) spectroscopy has been performed on a nitroxide spin-labeled peptide in fluid aqueous solution. Hydroxylamine 90-99 spindlin 1 Homo sapiens 100-104 10423365-4 1999 Simulation of the temperature-dependent spectral lineshapes reveals the existence of local anisotropic motion about the nitroxide N-O bond with a motional anisotropy tau( perpendicular)/tau( parallel) ( identical with N) approaching 2.6 at 306 K. Comparison with previous work on rigidly labeled peptides suggests that the spin label is reorienting about its side-chain tether. Hydroxylamine 120-129 spindlin 1 Homo sapiens 323-327 10773169-2 2000 The enhanced Zeeman splitting leads to spectra with resolved g-tensor components of the nitroxide spin label. Hydroxylamine 88-97 spindlin 1 Homo sapiens 98-102 10773169-6 2000 The enhanced sensitivity of high-field EPR with respect to anisotropic reorientational motion of nitroxides allows the characterization of different motional modes for spin labels bound to positions 167 and 170. Hydroxylamine 97-107 spindlin 1 Homo sapiens 168-172 11674206-5 1999 Superoxide trapping by DEPPPO gave a persistent nitroxide spin adduct, and its half-time life was measured and compared to that of the DEPMPO analogue. Hydroxylamine 48-57 spindlin 1 Homo sapiens 58-62 2157417-0 1990 Subcellular distribution of a nitroxide spin-labeled netropsin-acridine hybrid in living KB cells: Electron Spin Resonance Study. Hydroxylamine 30-39 spindlin 1 Homo sapiens 40-44 7793976-2 1995 The electron spin resonance (ESR) spectrum of SSL bound to LDL indicated that the nitroxide moiety was relatively mobile. Hydroxylamine 82-91 spindlin 1 Homo sapiens 13-17 7793976-5 1995 During Cu(2+)-initiated oxidation of spin-labeled LDL, nitroxide labels located in a hydrophobic environment were predominantly degraded. Hydroxylamine 55-64 spindlin 1 Homo sapiens 37-41 8396553-3 1993 During EPR studies of chemical, biochemical, and cellular processes involving free radicals, molecular oxygen has significant magnetic influence on the EPR signal intensity of the free radical species under investigation in addition to affecting the rates of production of the primary species and the stability of the spin adduct nitroxides. Hydroxylamine 330-340 spindlin 1 Homo sapiens 318-322 8396553-5 1993 In the present study, the effects of oxygen and ferricyanide on the EPR signal height of stable and persistent spin adduct nitroxides at commonly employed microwave powers were examined. Hydroxylamine 123-133 spindlin 1 Homo sapiens 111-115 1663907-3 1991 The hydroxylamine is oxidized to the radical t-BuN(O)H (2) which is converted into the spin trap 2-methyl-2-nitrosopropane (3). Hydroxylamine 4-17 spindlin 1 Homo sapiens 87-91 9245357-0 1997 A Double-Modulation ESR Study of Internal Dynamics in the Glassy Polymer Matrix Detected by a Nitroxide Spin Probe The double-modulation ESR spin-probe technique was employed to study slow-motional dynamics in amorphous polymer matrices of polybutadiene, polyisobutylene, poly(methyl methacrylate), and paraffin. Hydroxylamine 94-103 spindlin 1 Homo sapiens 104-108 9245357-0 1997 A Double-Modulation ESR Study of Internal Dynamics in the Glassy Polymer Matrix Detected by a Nitroxide Spin Probe The double-modulation ESR spin-probe technique was employed to study slow-motional dynamics in amorphous polymer matrices of polybutadiene, polyisobutylene, poly(methyl methacrylate), and paraffin. Hydroxylamine 94-103 spindlin 1 Homo sapiens 141-145 27405952-0 1996 Structural dependence of nitroxide spin labels and nitroxide spin adducts on their reducibility by ascorbate ion. Hydroxylamine 25-34 spindlin 1 Homo sapiens 35-39 27405952-0 1996 Structural dependence of nitroxide spin labels and nitroxide spin adducts on their reducibility by ascorbate ion. Hydroxylamine 51-60 spindlin 1 Homo sapiens 61-65 7578089-1 1995 Six nitroxide spin-labeled psoralen derivative have been synthesized and evaluated as probes for structural and dynamic studies. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 7578089-3 1995 Comparison of the general line shape features of the observed electron paramagnetic resonance (EPR) spectra of several duplexes ranging in size from 8 to 46 base pairs with simulated EPR spectra indicate that the nitroxide spin-label probe reports the global tumbling motion of the oligomers. Hydroxylamine 213-222 spindlin 1 Homo sapiens 223-227 7981788-2 1994 The spin-labeled nitrosoureas, triazenes and their precursor nitroxides exhibited excellent SSA, whereas clinically used nitrosourea and triazene, which do not contain the nitroxide moiety, did not show any SSA. Hydroxylamine 61-71 spindlin 1 Homo sapiens 4-8 7981788-2 1994 The spin-labeled nitrosoureas, triazenes and their precursor nitroxides exhibited excellent SSA, whereas clinically used nitrosourea and triazene, which do not contain the nitroxide moiety, did not show any SSA. Hydroxylamine 61-70 spindlin 1 Homo sapiens 4-8 1664324-2 1991 The results can be interpreted in terms of a two dimensional translational diffusion of the nitroxide tips of the spin labels along the protein surface within restricted surface areas. Hydroxylamine 92-101 spindlin 1 Homo sapiens 114-118 2166571-1 1990 A spin-label (P-OPC) composed of the nitroxide-containing ring proxyl linked at the C1 position of the intercalating fluorescent chromophore oxazolopyridocarbazole (OPC) has been synthesized. Hydroxylamine 37-46 spindlin 1 Homo sapiens 2-6 2157417-0 1990 Subcellular distribution of a nitroxide spin-labeled netropsin-acridine hybrid in living KB cells: Electron Spin Resonance Study. Hydroxylamine 30-39 spindlin 1 Homo sapiens 108-112 2154454-5 1990 This nitroxide, which we have assigned as PBN/.OCH3, appears to be an oxygen-centered radical derived from the spin trapping of the reaction product of O2 and methyl radical. Hydroxylamine 5-14 spindlin 1 Homo sapiens 111-115 2167277-0 1990 Principles of the metabolism of nitroxides and their implications for spin trapping. Hydroxylamine 32-42 spindlin 1 Homo sapiens 70-74 2167277-1 1990 A review of the principal interactions of nitroxides with cells suggests that if these same phenomena occur with spin adducts the result could be considerable experimental confusion and error. Hydroxylamine 42-52 spindlin 1 Homo sapiens 113-117 2167277-3 1990 In addition, shuttling of electrons between nitroxides and hydroxylamines also very significantly could alter the amounts and types of spin adducts that are observed. Hydroxylamine 44-54 spindlin 1 Homo sapiens 135-139 35509368-2 2022 Here, we consider the case of two methyl groups bound to the same sp 3 -hybridized atom, which is important in the context of common nitroxide spin labels. Hydroxylamine 135-144 spindlin 1 Homo sapiens 145-149 34649176-5 2021 Even modest values of J are large relative to the separation between trityl and nitroxide resonances for some nitrogen nuclear spin state. Hydroxylamine 80-89 spindlin 1 Homo sapiens 127-131 35226325-5 2022 Highly structured, several hundred nucleotides long RNAs with two nitroxide spin labels at specific positions can be prepared by this method. Hydroxylamine 66-75 spindlin 1 Homo sapiens 76-80