PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25891767-9 2014 Selective inhibitors of Rho kinase, ERK1/2, CaMKK/AMPK, and CaMKII each reduced carbachol-induced contraction in the innervated muscle strips. Carbachol 80-89 mitogen activated protein kinase 3 Rattus norvegicus 36-42 7626007-4 1995 Activation of MAPK and MAPK kinase (MEK) by carbachol returned to control levels within 30-60 min, whereas activation by FCS was more sustained. Carbachol 44-53 mitogen activated protein kinase 3 Rattus norvegicus 14-18 7626007-4 1995 Activation of MAPK and MAPK kinase (MEK) by carbachol returned to control levels within 30-60 min, whereas activation by FCS was more sustained. Carbachol 44-53 mitogen activated protein kinase 3 Rattus norvegicus 23-27 7626007-5 1995 FPLC on Mono Q showed that carbachol and FCS activated two peaks of MEK and two peaks of MAPK (p42MAPK and p44MAPK). Carbachol 27-36 mitogen activated protein kinase 3 Rattus norvegicus 89-93 7626007-5 1995 FPLC on Mono Q showed that carbachol and FCS activated two peaks of MEK and two peaks of MAPK (p42MAPK and p44MAPK). Carbachol 27-36 mitogen activated protein kinase 3 Rattus norvegicus 107-114 7626007-6 1995 Pretreatment of cells with PTX for 24 h inhibited the activation of MAPK by carbachol, FCS and lysophosphatidic acid, but not that by endothelin-1, phenylephrine or isoprenaline. Carbachol 76-85 mitogen activated protein kinase 3 Rattus norvegicus 68-72 26606130-3 2016 AF710B exhibits an allosteric agonistic profile on the M1 muscarinic receptor; very low concentrations of AF710B significantly potentiated the binding and efficacy of carbachol on M1 receptors and their downstream effects (p-ERK1/2, p-CREB). Carbachol 167-176 mitogen activated protein kinase 3 Rattus norvegicus 225-231 25891767-11 2014 Thus unlike previously reported for isolated muscle cells where CaMKII and ERK1/2 are not involved in contraction, we conclude that the regulation of carbachol-induced contraction in innervated longitudinal muscle strips involves the interplay of Rho kinase, ERK1/2, CaMKK/AMPK, and CAMKII. Carbachol 150-159 mitogen activated protein kinase 3 Rattus norvegicus 259-265 22975404-12 2012 Inhibitors against the EGF receptor and ERK 1/2 also blocked Cch-stimulated mucin secretion. Carbachol 61-64 mitogen activated protein kinase 3 Rattus norvegicus 40-47 20871620-8 2010 In addition, treatment of endothelial cells with carbachol decreased phosphor-ERK1/2 and phosphor-JNK. Carbachol 49-58 mitogen activated protein kinase 3 Rattus norvegicus 78-84 19801823-5 2009 Carbacol and nerve growth factor (NGF), which are ERK1/2 activators, protected the neurons after CPF withdrawal, while atropine and PD98059, which are ERK1/2 inhibitors, exacerbated the cytotoxicity, indicating the involvement of inhibition of ERK1/2 phosphorylation in CPF-induced delayed cytotoxicity. Carbachol 0-8 mitogen activated protein kinase 3 Rattus norvegicus 50-56 19673741-4 2009 The cholinergic agonist carbachol has been shown to induce axonal outgrowth through intracellular calcium mobilization, protein kinase C (PKC) activation, and ERK1/2 phosphorylation. Carbachol 24-33 mitogen activated protein kinase 3 Rattus norvegicus 159-165 19673741-13 2009 CONCLUSIONS: Ethanol inhibited carbachol-induced neurite outgrowth by inhibiting PKC and ERK1/2 activation. Carbachol 31-40 mitogen activated protein kinase 3 Rattus norvegicus 89-95 17919561-10 2007 Carbachol-stimulated MAPK activity was inhibited by three PKC inhibitors, calphostin C, chelethyrine, and staurosporine. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 21-25 19190235-8 2009 These data suggest an involvement of Ca(2+), PKC, and ERK1/2 in carbachol-induced axonal growth. Carbachol 64-73 mitogen activated protein kinase 3 Rattus norvegicus 54-60 19190235-9 2009 Carbachol indeed increased the release of Ca(2+) from intracellular stores and induced PKC and ERK1/2 activation. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 95-101 19190235-10 2009 Additional experiments showed that PKC, but not Ca(2+), is involved in carbachol-induced ERK1/2 activation. Carbachol 71-80 mitogen activated protein kinase 3 Rattus norvegicus 89-95 18632735-8 2008 These results suggest that carbachol-initiated AMPK activation can produce a negative feedback on ERK1/2 signaling in response to submaximal muscarinic receptor activation and that increases in fluid secretion can modulate receptor-initiated signaling events indirectly by producing ion transport-dependent decreases in ATP. Carbachol 27-36 mitogen activated protein kinase 3 Rattus norvegicus 98-104 19190235-7 2009 In addition, down-regulation of ERK1/2 with small interfering RNA abolished the neuritogenic effect of carbachol. Carbachol 103-112 mitogen activated protein kinase 3 Rattus norvegicus 32-38 18772167-6 2008 Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K(-Thr389) and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. Carbachol 45-54 mitogen activated protein kinase 3 Rattus norvegicus 105-110 18772167-6 2008 Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K(-Thr389) and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. Carbachol 56-59 mitogen activated protein kinase 3 Rattus norvegicus 105-110 18632735-1 2008 We previously found that the phosphorylation of ERK1/2 by submaximal concentrations of the muscarinic receptor ligand carbachol was potentiated in rat parotid acinar cells exposed to ouabain, a cardiac glycoside that inhibits the Na-K-ATPase. Carbachol 118-127 mitogen activated protein kinase 3 Rattus norvegicus 48-54 17687004-10 2007 DBcAMP and VIP inhibited carbachol- and EGF-stimulated MAPK activity. Carbachol 25-34 mitogen activated protein kinase 3 Rattus norvegicus 55-59 12692900-6 2003 Both extracellular signal-regulated kinase (Erk1/2) and CREB were significantly activated after CCh treatment indicated by increases in phosphorylation of these two proteins. Carbachol 96-99 mitogen activated protein kinase 3 Rattus norvegicus 44-50 16236826-0 2006 Ouabain potentiates the activation of ERK1/2 by carbachol in parotid gland epithelial cells; inhibition of ERK1/2 reduces Na(+)-K(+)-ATPase activity. Carbachol 48-57 mitogen activated protein kinase 3 Rattus norvegicus 38-44 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 99-108 mitogen activated protein kinase 3 Rattus norvegicus 9-15 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 99-108 mitogen activated protein kinase 3 Rattus norvegicus 151-157 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 221-230 mitogen activated protein kinase 3 Rattus norvegicus 9-15 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 221-230 mitogen activated protein kinase 3 Rattus norvegicus 151-157 16236826-11 2006 In addition, inhibition of ERK1/2 reduces Na(+)-K(+)-ATPase activity (measured as stimulation of Qo(2) by carbachol and the cationophore nystatin). Carbachol 106-115 mitogen activated protein kinase 3 Rattus norvegicus 27-33 16877402-7 2006 Inhibition of PKC partially inhibited carbachol-stimulated MAPK activation while completely inhibiting phenylephrine- and EGF-stimulated MAPK activation. Carbachol 38-47 mitogen activated protein kinase 3 Rattus norvegicus 59-63 16877402-8 2006 Chelation of Ca(2+) also partially inhibited carbachol-stimulated MAPK with no effect on phenylephrine- and EGF-stimulated MAPK activation. Carbachol 45-54 mitogen activated protein kinase 3 Rattus norvegicus 66-70 12692900-7 2003 The c-Src inhibitor PP1 abolished the CCh-induced activation of Erk1/2 and CREB in a dose-dependent manner. Carbachol 38-41 mitogen activated protein kinase 3 Rattus norvegicus 64-70 12020766-8 2002 Pretreatment of the cells with PP2 markedly decreased Cch-induced ERK1/2 phosphorylation, suggesting a role of Src family of tyrosine kinases in the signal transduction pathway involved in ERK1/2 activation by mAChR. Carbachol 54-57 mitogen activated protein kinase 3 Rattus norvegicus 66-72 12020766-1 2002 Carbachol (Cch), a muscarinic acetylcholine receptors (mAChR) agonist, produces time- and dose-dependent increases in mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in nondifferentiated Fischer rat thyroid (FRT) epithelial cells. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 190-194 12020766-1 2002 Carbachol (Cch), a muscarinic acetylcholine receptors (mAChR) agonist, produces time- and dose-dependent increases in mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in nondifferentiated Fischer rat thyroid (FRT) epithelial cells. Carbachol 11-14 mitogen activated protein kinase 3 Rattus norvegicus 190-194 12020766-2 2002 Cells pretreatment with the selective phospholipase C inhibitor U73122 resulted in a decrease of Cch-stimulated ERK1/2 phosphorylation. Carbachol 97-100 mitogen activated protein kinase 3 Rattus norvegicus 112-118 12020766-6 2002 Additionally, Cch-induced ERK1/2 phosphorylation was reduced after either inhibition of Ca(2+) influx or intracellular Ca(2+) release. Carbachol 14-17 mitogen activated protein kinase 3 Rattus norvegicus 26-32 12020766-7 2002 Nevertheless, Cch-mediated ERK1/2 activation was genistein sensitive, indicating the involvement of protein tyrosine kinases on the downstream signalling of mAChR. Carbachol 14-17 mitogen activated protein kinase 3 Rattus norvegicus 27-33 12020766-8 2002 Pretreatment of the cells with PP2 markedly decreased Cch-induced ERK1/2 phosphorylation, suggesting a role of Src family of tyrosine kinases in the signal transduction pathway involved in ERK1/2 activation by mAChR. Carbachol 54-57 mitogen activated protein kinase 3 Rattus norvegicus 189-195 11222647-4 2001 We demonstrate that stimulation of these mAChRs with carbachol, a muscarinic agonist, activated extracellular-regulated kinases (Erk1/2) and phosphatidylinositol-3 kinase (PI-3K). Carbachol 53-62 mitogen activated protein kinase 3 Rattus norvegicus 129-135 11292831-2 2001 We first show that activation of ERK1/2 by the M(1) mAChR agonist carbachol takes place primarily via a Ras-independent pathway that depends largely upon Rap1, another small GTP-binding protein in the Ras family. Carbachol 66-75 mitogen activated protein kinase 3 Rattus norvegicus 33-39 11714707-3 2002 Applying carbachol to the contralateral hippocampal slices from ischemic rats increased the phosphorylation of ERK1/2 but did not increase phosphorylation in the ipsilateral hippocampus. Carbachol 9-18 mitogen activated protein kinase 3 Rattus norvegicus 111-117 10519505-3 1999 Carbachol-induced tyrosine-phosphorylated proteins of 44 and 42 kd were determined by Western blot analysis and identified as activated ERK1 and ERK2 using anti-ERK antibody. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 136-140