PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 6470036-1 1984 The effect of the chemotatic peptide, N-formylmethionylleucylphenylalanine (FMLP), on actin conformation in human neutrophils (PMN) was studied by flow cytometry using fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD)-phallacidin to quantitate cellular F-actin content. phallacidin 216-227 formyl peptide receptor 1 Homo sapiens 76-80 8409758-7 1993 Similarly, resting and fMLP-stimulated levels of F-actin in cancer patients" leukocytes as assessed by NBD-phallacidin staining did not differ significantly from those of normal leukocytes. phallacidin 107-118 formyl peptide receptor 1 Homo sapiens 23-27 1665427-4 1991 In investigations of the dynamic aspect of neutrophil actin during priming and stimulation with FMLP the content of F-actin has been measured with and NBD (nitrobenz-2-oxa-1,3-diazole)-phallacidin assay. phallacidin 185-196 formyl peptide receptor 1 Homo sapiens 96-100 6470036-4 1984 Under fluorescence microscopy, NBD-phallacidin stained, unstimulated PMN had faint homogeneous cytoplasmic fluorescence while cells exposed to FMLP for 30 s prior to NBD-phallacidin staining had accentuated subcortical fluorescence. phallacidin 170-181 formyl peptide receptor 1 Homo sapiens 143-147 6232281-10 1984 FACS analysis of NBD-phallacidin-stained cells shows that the relative F-actin content of neutrophils stimulated with 10(-11)-10(-8)M fMLP increases twofold and remains increased at concentrations greater than 10(-8)M fMLP. phallacidin 21-32 formyl peptide receptor 1 Homo sapiens 134-138 6232281-11 1984 Therefore, the fMLP-induced increase in F-actin content of neutrophils as determined by FACS analysis of NBD-phallacidin-stained cells coincides with a decrease in G-actin and correlates with increased mROL of neutrophils under some (10(-11)-10(-8)M fMLP) but not all (greater than 10(-8)M fMLP) conditions of stimulation. phallacidin 109-120 formyl peptide receptor 1 Homo sapiens 15-19 6232281-9 1984 Since NBD-phallacidin binds specifically to F-actin, the increase in fluorescent staining of cells likely reflects an increase in the F-actin content of fMLP-stimulated cells. phallacidin 10-21 formyl peptide receptor 1 Homo sapiens 153-157 6232281-8 1984 The NBD-phallacidin staining is not due to phagocytosis, is inhibited by phalloidin, requires cell permeabilization, and is saturable at NBD-phallacidin concentrations greater than 10(-7)M. The fMLP-induced increase in NBD-phallacidin staining occurs rapidly (less than 2 min), is temperature dependent, and is not due to cell aggregation. phallacidin 8-19 formyl peptide receptor 1 Homo sapiens 194-198 6232281-8 1984 The NBD-phallacidin staining is not due to phagocytosis, is inhibited by phalloidin, requires cell permeabilization, and is saturable at NBD-phallacidin concentrations greater than 10(-7)M. The fMLP-induced increase in NBD-phallacidin staining occurs rapidly (less than 2 min), is temperature dependent, and is not due to cell aggregation. phallacidin 141-152 formyl peptide receptor 1 Homo sapiens 194-198 6232281-8 1984 The NBD-phallacidin staining is not due to phagocytosis, is inhibited by phalloidin, requires cell permeabilization, and is saturable at NBD-phallacidin concentrations greater than 10(-7)M. The fMLP-induced increase in NBD-phallacidin staining occurs rapidly (less than 2 min), is temperature dependent, and is not due to cell aggregation. phallacidin 141-152 formyl peptide receptor 1 Homo sapiens 194-198