PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10564383-0 1999 Human striosomes are enriched in 5-HT2A receptors: autoradiographical visualization with [3H]MDL100,907,[125I](+/-)DOI and [3H]ketanserin. Tritium 90-92 5-hydroxytryptamine receptor 2A Homo sapiens 33-39 18468904-0 2008 High specific activity tritium-labeled N-(2-methoxybenzyl)-2,5-dimethoxy-4-iodophenethylamine (INBMeO): a high-affinity 5-HT2A receptor-selective agonist radioligand. Tritium 23-30 5-hydroxytryptamine receptor 2A Homo sapiens 120-135 17238805-4 2006 [3H]-5-HT and [3H]-ketanserin autoradiography on sections of human eyes revealed a high density of 5-HT2 receptor binding sites in the iris, ciliary epithelium, and longitudinal CM. Tritium 1-3 5-hydroxytryptamine receptor 2A Homo sapiens 99-113 17238805-4 2006 [3H]-5-HT and [3H]-ketanserin autoradiography on sections of human eyes revealed a high density of 5-HT2 receptor binding sites in the iris, ciliary epithelium, and longitudinal CM. Tritium 15-17 5-hydroxytryptamine receptor 2A Homo sapiens 99-113 11135236-0 2001 Mapping of 5-HT2A receptors and their mRNA in monkey brain: [3H]MDL100,907 autoradiography and in situ hybridization studies. Tritium 61-63 5-hydroxytryptamine receptor 2A Homo sapiens 11-17 11135236-4 2001 5-HT2A receptor mRNA was detected in layers III and IV, and in some cases also in layers II and V. In intra- and extra-calcarine areas of striate cortex a five-banded pattern was distinguished, with layers III and IVc-V showing the highest densities of [3H]MDL100,907 labeling. Tritium 254-256 5-hydroxytryptamine receptor 2A Homo sapiens 0-15 11135236-8 2001 Some differences were noted between the distribution of [3H]MDL100,907-labeled 5-HT2A receptors in monkey brain and the previously reported distribution of these receptors in human brain: absence of striosome labeling in monkey striatum and different patterns of neocortical labeling. Tritium 57-59 5-hydroxytryptamine receptor 2A Homo sapiens 79-85 10940539-1 2000 A large series of beta-carbolines was examined for their ability to bind at [3H]agonist-labeled 5-HT(2A) serotonin receptors. Tritium 77-79 5-hydroxytryptamine receptor 2A Homo sapiens 96-103 12798981-1 2003 The 5-HT2A receptor binding parameters using [3H]ketanserine and its intracellular signal inositol 1,4,5 trisphosphate (IP3) concentrations were determined in platelets from schizophrenic patients so as to assess differences with respect to a control group and to a standardized antipsychotic drug treatment. Tritium 46-48 5-hydroxytryptamine receptor 2A Homo sapiens 4-19 10564383-0 1999 Human striosomes are enriched in 5-HT2A receptors: autoradiographical visualization with [3H]MDL100,907,[125I](+/-)DOI and [3H]ketanserin. Tritium 124-126 5-hydroxytryptamine receptor 2A Homo sapiens 33-39 10564383-1 1999 5-HT2A receptors have been visualized with [3H]MDL100,907 in selected human brain areas by autoradiography. Tritium 44-46 5-hydroxytryptamine receptor 2A Homo sapiens 0-6 10564383-7 1999 These results confirm the presence of 5-HT2A receptors in human striosomes and in those areas where [3H]ketanserin presented a high nonspecific binding, and they highlight the advantage of using [3H]MDL100,907 to visualize these receptors. Tritium 101-103 5-hydroxytryptamine receptor 2A Homo sapiens 38-44 10564383-7 1999 These results confirm the presence of 5-HT2A receptors in human striosomes and in those areas where [3H]ketanserin presented a high nonspecific binding, and they highlight the advantage of using [3H]MDL100,907 to visualize these receptors. Tritium 196-198 5-hydroxytryptamine receptor 2A Homo sapiens 38-44 9178960-4 1997 In conclusion, the 5-HT2A receptor, as labeled by [3H]ketanserin, would seem to consist of a homogenous population of binding sites and to be equally distributed in human prefronto-cortical, limbic and extrapyramidal structures. Tritium 51-53 5-hydroxytryptamine receptor 2A Homo sapiens 19-34 10509892-3 1999 Multiple regression analyses indicated that the responses to 1 micromol/l serotonin, but not the responses to thrombin, were significantly correlated with the serotonin2A receptor density measured using [3H]LSD as radioligand. Tritium 204-206 5-hydroxytryptamine receptor 2A Homo sapiens 159-179 10090630-9 1999 Acute endurance exercise reduced the density of platelet 5-HT2A receptor [3H]ketanserin binding sites at I and II (P < 0.05). Tritium 74-76 5-hydroxytryptamine receptor 2A Homo sapiens 57-72 10049714-0 1999 Dopamine D4-like binding sites labeled by [3H]nemonapride include substantial serotonin 5-HT2A receptors in primate cerebral cortex. Tritium 43-45 5-hydroxytryptamine receptor 2A Homo sapiens 88-94 9833645-0 1998 [3H]MDL 100,907 labels 5-HT2A serotonin receptors selectively in primate brain. Tritium 1-3 5-hydroxytryptamine receptor 2A Homo sapiens 23-29 9833645-7 1998 The present results confirm [3H]MDL 100,907 as the radioligand of choice at present for the autoradiographic visualization of 5-HT2A receptors in mammalian brain including post-mortem human brain. Tritium 29-31 5-hydroxytryptamine receptor 2A Homo sapiens 126-132 10081968-4 1999 Twelve healthy volunteers had the 5-HT2A receptors on their platelet membranes assessed in vitro using [3H]lysergic acid diethylamide ([3H]LSD) and their central 5-HT2A receptors measured in vivo using [18F]setoperone and positron emission tomography (PET) imaging. Tritium 104-106 5-hydroxytryptamine receptor 2A Homo sapiens 34-40 9831908-5 1998 5-HT stimulated [3H]-inositol phosphate ([3H]-IPx) production at both the 5-HT2C (pEC50=8.03+/-0.15) and 5-HT2A receptors (pEC50=7.15+/-0.08), with maximal responses occurring after exposure to 1 microM and 10 microM 5-HT, respectively. Tritium 17-19 5-hydroxytryptamine receptor 2A Homo sapiens 105-111 9831908-5 1998 5-HT stimulated [3H]-inositol phosphate ([3H]-IPx) production at both the 5-HT2C (pEC50=8.03+/-0.15) and 5-HT2A receptors (pEC50=7.15+/-0.08), with maximal responses occurring after exposure to 1 microM and 10 microM 5-HT, respectively. Tritium 42-44 5-hydroxytryptamine receptor 2A Homo sapiens 105-111 9754440-5 1998 When all four brain regions were taken together, a down-regulation was noted between presynaptic [3H]imipramine binding and the postsynaptic 5-HT2A receptor (r = -0.40; P = 0.0013) in the control group. Tritium 98-100 5-hydroxytryptamine receptor 2A Homo sapiens 141-156 9282925-2 1997 Incubation of cells expressing the 5-HT2A receptor with mianserin (100 nM) for 24 h caused a significant decrease (48%) in the binding capacity of [3H] ketanserin. Tritium 148-150 5-hydroxytryptamine receptor 2A Homo sapiens 35-50 8549648-2 1995 In [3H]5-HT competition assays, the classical 5-HT2A receptor antagonists, ritanserin and ketanserin, displayed moderate affinity (pKi = 7.30 and 7.17, respectively) and marked selectivity (22- and 71-fold, respectively) for the recombinant human 5-HT1D alpha subtype relative to the 5-HT1D beta receptor. Tritium 4-6 5-hydroxytryptamine receptor 2A Homo sapiens 46-61 8534270-2 1996 Because 5-HT has high affinity for the 5-HT2A receptor when displacing [3H]-DOB, the purpose of the present study was to determine whether or not the receptor could be labelled with [3H]-5-HT and what would be the effect of labelling the receptor with various radioligands having differing efficacies at the receptor. Tritium 72-74 5-hydroxytryptamine receptor 2A Homo sapiens 39-54 8534270-3 1996 Consequently, the human 5-HT2A receptor stably expressed in NIH 3T3 cells was radiolabelled with the endogenous agonist [3H]-5-HT, the partial agonist [3H]-DOB, and the antagonist [3H]-ketanserin. Tritium 121-123 5-hydroxytryptamine receptor 2A Homo sapiens 24-39 8534270-3 1996 Consequently, the human 5-HT2A receptor stably expressed in NIH 3T3 cells was radiolabelled with the endogenous agonist [3H]-5-HT, the partial agonist [3H]-DOB, and the antagonist [3H]-ketanserin. Tritium 152-154 5-hydroxytryptamine receptor 2A Homo sapiens 24-39 8534270-3 1996 Consequently, the human 5-HT2A receptor stably expressed in NIH 3T3 cells was radiolabelled with the endogenous agonist [3H]-5-HT, the partial agonist [3H]-DOB, and the antagonist [3H]-ketanserin. Tritium 152-154 5-hydroxytryptamine receptor 2A Homo sapiens 24-39 9094146-0 1997 Serotonin 5-HT2A receptor binding in platelets from healthy subjects as studied by [3H]-lysergic acid diethylamide ([3H]-LSD): intra- and interindividual variability. Tritium 84-86 5-hydroxytryptamine receptor 2A Homo sapiens 0-25 9094146-0 1997 Serotonin 5-HT2A receptor binding in platelets from healthy subjects as studied by [3H]-lysergic acid diethylamide ([3H]-LSD): intra- and interindividual variability. Tritium 117-119 5-hydroxytryptamine receptor 2A Homo sapiens 0-25 2894321-3 1988 A method has been developed to study 5-HT2 receptor binding sites on platelets utilising [3H]-LSD and more recently 125I/LSD. Tritium 90-92 5-hydroxytryptamine receptor 2A Homo sapiens 37-51 1435968-2 1992 Both the discriminative stimulus and human hallucinogenic potencies of the agonists are significantly correlated with 5-HT2 receptor affinity when [3H]-ketanserin is used as radioligand (Glennon et al., 1984). Tritium 148-150 5-hydroxytryptamine receptor 2A Homo sapiens 118-132 2233697-8 1990 These data on a single human 5-HT2 receptor cDNA expressed in two different transfection host cells indicate that [3H]DOB and [3H]ketanserin binding reside on the same gene product, apparently interacting with agonist and antagonist conformations of a single human 5-HT2 receptor protein. Tritium 115-117 5-hydroxytryptamine receptor 2A Homo sapiens 29-43 2233697-8 1990 These data on a single human 5-HT2 receptor cDNA expressed in two different transfection host cells indicate that [3H]DOB and [3H]ketanserin binding reside on the same gene product, apparently interacting with agonist and antagonist conformations of a single human 5-HT2 receptor protein. Tritium 115-117 5-hydroxytryptamine receptor 2A Homo sapiens 265-279 2233697-8 1990 These data on a single human 5-HT2 receptor cDNA expressed in two different transfection host cells indicate that [3H]DOB and [3H]ketanserin binding reside on the same gene product, apparently interacting with agonist and antagonist conformations of a single human 5-HT2 receptor protein. Tritium 127-129 5-hydroxytryptamine receptor 2A Homo sapiens 29-43 2310804-1 1990 The 5HT2 receptor has been studied using quantitative tritium film autoradiography in the postmortem frontal cortex of 15 cases suffering from major depression and controls, matched for age, gender, postmortem delay, and storage time. Tritium 54-61 5-hydroxytryptamine receptor 2A Homo sapiens 4-17 2723656-4 1989 Displacement of specific [125I]iodoLSD binding indicated a typical 5-HT2 receptor inhibition profile, which demonstrated a significant linear correlation (r = 0.97, p less than 0.001, n = 17) with that observed using [3H]ketanserin. Tritium 218-220 5-hydroxytryptamine receptor 2A Homo sapiens 67-81 8329169-8 1993 Therefore, we recommend [3H]LSD as ligand of choice to study platelet 5-HT2 receptor binding in humans. Tritium 25-27 5-hydroxytryptamine receptor 2A Homo sapiens 70-84 2078270-6 1990 Second, the single protein encoded by the human 5-HT2 receptor gene is capable of binding both [3H]DOB and [3H]ketanserin. Tritium 96-98 5-hydroxytryptamine receptor 2A Homo sapiens 48-62 2078270-6 1990 Second, the single protein encoded by the human 5-HT2 receptor gene is capable of binding both [3H]DOB and [3H]ketanserin. Tritium 108-110 5-hydroxytryptamine receptor 2A Homo sapiens 48-62 1969761-6 1990 Evidence from binding studies indicates that MDMA has a micromolar affinity for the 5-HT2 receptor, and our studies in culture showed that ketanserin, a specific 5-HT2 antagonist, was effective at attenuating the effects of S(+)-MDMA on the development of the [3H]5-HT uptake capacity by the cultured raphe neurons. Tritium 261-263 5-hydroxytryptamine receptor 2A Homo sapiens 84-98 3359270-1 1988 5-HT2 receptor binding sites were measured (by saturation binding of [3H]ketanserin) in brain tissue obtained at postmortem from 19 suicide victims with definite evidence of depression and 19 sex and age-matched control subjects. Tritium 70-72 5-hydroxytryptamine receptor 2A Homo sapiens 0-14 32311347-6 2020 These substitutions reduced the binding of the 5-HT2A receptor to [3H]lysergic acid diethylamide ([3H]LSD) and impeded the 5-HT2A receptor-mediated activation of phospholipase C (PLC). Tritium 67-69 5-hydroxytryptamine receptor 2A Homo sapiens 47-62 3663239-5 1987 The apparent 5-HT1 receptor selectivity of agonist compounds was found to be 50- to 100-fold higher when the 5-HT2 receptor affinity was determined using the antagonist radioligand [3H]ketanserin than when the agonist radioligand [3H]DOB was used. Tritium 182-184 5-hydroxytryptamine receptor 2A Homo sapiens 109-123