PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10073755-5 1999 Using an in vitro 3H-labeled elastin degradation assay, elastin-degrading enzyme activity was only observed in the bacteria-conditioned medium from an isolate of Pseudomonas aeruginosa. Tritium 18-20 elastin Homo sapiens 29-36 10208280-4 1999 Moreover, 100-200 microM suramin stimulates [3H]-thymidine incorporation by those tropoelastin-producing glioma cell lines, but not by A 2058 melanoma cells, which do not produce elastin. Tritium 45-47 elastin Homo sapiens 82-94 10073755-5 1999 Using an in vitro 3H-labeled elastin degradation assay, elastin-degrading enzyme activity was only observed in the bacteria-conditioned medium from an isolate of Pseudomonas aeruginosa. Tritium 18-20 elastin Homo sapiens 56-63 9596414-4 1998 Astrocytoma cells also express the cell surface 67-kDa elastin binding protein (EBP), which binds elastin degradation products, leading to the upregulation of cyclin A and cdk2 and increased incorporation of [3H]-thymidine. Tritium 209-211 elastin Homo sapiens 55-62 7977651-4 1994 With insoluble [3H] elastin as a substrate, elastin-degrading activity could be detected in the culture medium. Tritium 16-18 elastin Homo sapiens 20-27 9397158-3 1998 The mechanism involves tethering of the factor to SMC, since [3H]-elastin pre-incubated with serum or endothelial cell (EC)-conditioned medium or SMC pre-treated with serum accelerates binding of elastin and tyrosine-kinase related elastase activity. Tritium 62-64 elastin Homo sapiens 66-73 9397158-3 1998 The mechanism involves tethering of the factor to SMC, since [3H]-elastin pre-incubated with serum or endothelial cell (EC)-conditioned medium or SMC pre-treated with serum accelerates binding of elastin and tyrosine-kinase related elastase activity. Tritium 62-64 elastin Homo sapiens 196-203 8985148-4 1996 Lowering the growth temperature, and reducing the amount of inducer, resulted in the production of soluble LO, which was active on a degrees [3H]lysine-labeled elastin substrate. Tritium 142-144 elastin Homo sapiens 160-167 8967510-7 1996 Solubilization of lung elastin with PPE produced a residue that exhibited inhibitory capacity toward HLE when either 3H-labeled aorta elastin or succinyl trialanine nitroanilide was used as a substrate. Tritium 117-119 elastin Homo sapiens 23-30 8967510-7 1996 Solubilization of lung elastin with PPE produced a residue that exhibited inhibitory capacity toward HLE when either 3H-labeled aorta elastin or succinyl trialanine nitroanilide was used as a substrate. Tritium 117-119 elastin Homo sapiens 134-141 7489243-4 1995 In nondenuded organ cultures, elastolytic activity assessed by using [3H]elastin increased sixfold at day 3 after initiation of the culture (P < .01), a time earlier than the previously published increase in intimal smooth muscle cells (ISMCs). Tritium 70-72 elastin Homo sapiens 73-80 7977651-4 1994 With insoluble [3H] elastin as a substrate, elastin-degrading activity could be detected in the culture medium. Tritium 16-18 elastin Homo sapiens 44-51 8043973-3 1994 AM were obtained by bronchoalveolar lavage from patients with emphysema and from patients with non obstructive chronic pulmonary diseases (non-COPD) and cultured under serum-free conditions in direct contact with 3H-labeled elastin particles. Tritium 213-215 elastin Homo sapiens 224-231 8043973-4 1994 Elastinolytic activity was calculated from the released radioactivity in culture supernatants and expressed as micrograms of 3H-elastin degraded x 10(-5) AM x 72 h-1. Tritium 125-127 elastin Homo sapiens 128-135 1721559-11 1991 Among these proteins, [3H]-kE was found to bind exclusively to a 65 kDa protein that could be eluted selectively from elastin fibres with a neutral buffer containing 100 mM lactose. Tritium 23-25 elastin Homo sapiens 118-125 1789930-6 1991 It cannot hydrolyse 3H-labelled insoluble elastin and apolipoprotein AII, but did cleave a dinitrophenyl-octapeptide as well as apolipoprotein AI to 25-kDa and 24-kDa fragments formed sequentially. Tritium 20-22 elastin Homo sapiens 42-49 8200109-2 1994 The data indicate that measured elastolytic activity in this assay system was dependent on the amount of 3H-elastin/culture well. Tritium 105-107 elastin Homo sapiens 108-115 8200109-3 1994 3H-elastin > 350 micrograms/well, in contrast to the < or = 200 micrograms/well commonly used in this assay system, resulted in optimal measurement of elastolytic activity that was linear with respect to culture time (up to 72 h examined) and was directly proportional to number of AM/well (up to 1.0 x 10(6) examined). Tritium 0-2 elastin Homo sapiens 3-10 8398177-4 1993 Tissue culture wells were coated with insoluble 3H-labeled elastin substrate. Tritium 48-50 elastin Homo sapiens 59-66 8398177-7 1993 Elastin degradation was determined as soluble 3H-elastin fragments released into the supernatants. Tritium 46-48 elastin Homo sapiens 0-7 8398177-7 1993 Elastin degradation was determined as soluble 3H-elastin fragments released into the supernatants. Tritium 46-48 elastin Homo sapiens 49-56 8323282-6 1993 Elastin and poly-L-lysine were labeled by reductive methylation of amino groups with [3H]HCHO prior to treatment with NO2 in aqueous solutions at physiological pH. Tritium 86-88 elastin Homo sapiens 0-7 8323282-7 1993 NO2 exposure of elastin resulted in the solubilization of 84% of the associated radioactivity of which 79% was identified as [3H]methyllysine by amino acid analysis. Tritium 126-128 elastin Homo sapiens 16-23 1721559-1 1991 3H-Labelled kappa-elastin peptides (kE:75 kDa molecular weight) were shown to bind to confluent human skin fibroblast (HSF) cultures in a time-dependent and saturable manner. Tritium 0-2 elastin Homo sapiens 18-25 1850424-9 1991 On a molar basis the recombinant 92-kDa type IV collagenase was approximately 30% as active as human leukocyte elastase in solubilizing 3H-labeled elastin. Tritium 136-138 elastin Homo sapiens 147-154 2215358-2 1990 The susceptibility of stable [3H] elastin-fatty acid complexes to the action of porcine pancreatic elastase (PPE) and to human neutrophil lysates over time was assessed. Tritium 30-32 elastin Homo sapiens 34-41 1988771-7 1991 Approximately 1% of dietary desmosine (ingested as [3H]elastin and [3H] desmosine) was excreted in the urine within 24 hours, contributing approximately 15% of UD while on a normal diet. Tritium 52-54 elastin Homo sapiens 55-62 3649236-5 1987 Elastase activity, measured by the hydrolysis of [3H]elastin, was highest in aneurysmal aortic homogenates, 92.1 +/- 43.7 U/mg protein (n = 18), falling to 46.9 +/- 13.3 U/mg protein (n = 13) in severely stenosed atherosclerotic aortic homogenates and 35.5 +/- 11.9 U/mg (n = 6) in grossly normal aortic homogenates. Tritium 50-52 elastin Homo sapiens 53-60 2159859-2 1990 Using soluble 3H-elastin as substrate in a cell culture assay we examined the ability of live, adherent human blood neutrophils and monocytes to release elastolytic activity following immune complex (IC) stimulation. Tritium 14-16 elastin Homo sapiens 17-24 1971160-1 1990 A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a [4,5-3H]-lysine-labeled elastin substrate. Tritium 172-174 elastin Homo sapiens 232-239 2512639-3 1989 The enzyme activity of live cells was measured by soluble [3H]elastin hydrolysis first under basic conditions and then after immune complex stimulation. Tritium 59-61 elastin Homo sapiens 62-69 3642713-0 1986 Degradation of solubilized [3H]elastin by intact human monocytes stimulated by immune complexes in vitro. Tritium 28-30 elastin Homo sapiens 31-38 3642713-2 1986 The substrate, solubilized [3H]elastin, is coated onto cell culture dishes. Tritium 28-30 elastin Homo sapiens 31-38 6918355-1 1982 The effect of three elastase inhibitors (MeO-Suc-Ala-Ala-Pro-Val-CH2Cl, eglin c and alpha 1-proteinase inhibitor) on the hydrolysis of [3H]elastin was determined. Tritium 136-138 elastin Homo sapiens 139-146 3008720-1 1986 Exposure of [3H]-lysine labeled elastin to either purified myeloperoxidase plus H2O2 and halides or human neutrophils plus phorbol myristate acetate resulted in oxidation of lysine side chains quantitated as 3H2O release. Tritium 13-15 elastin Homo sapiens 32-39 3161694-1 1985 Chymotrypsin can completely solubilize insoluble [3H]-labeled ligamentum nuchae elastin. Tritium 50-52 elastin Homo sapiens 80-87 3161694-6 1985 The sizes of [3H]elastin fragments produced by the elastolytic activity of chymotrypsin are similar to those produced by pancreatic elastase, and larger than those produced by trypsin. Tritium 14-16 elastin Homo sapiens 17-24 6568228-6 1984 However, macrophages cultured in 10% human serum and in contact with purified 3H-elastin degraded 4.7 micrograms elastin/10(6) cells per 24 h, as compared to less than 1 microgram/10(6) cells/24 h for neutrophils. Tritium 78-80 elastin Homo sapiens 81-88 6568228-6 1984 However, macrophages cultured in 10% human serum and in contact with purified 3H-elastin degraded 4.7 micrograms elastin/10(6) cells per 24 h, as compared to less than 1 microgram/10(6) cells/24 h for neutrophils. Tritium 78-80 elastin Homo sapiens 113-120 6561956-0 1984 Adsorbed soluble [3H]elastin as substrate for proteinase activity. Tritium 18-20 elastin Homo sapiens 21-28 6373133-1 1984 We developed an assay for measurement of elastolytic activity using insoluble 3H-labelled particulate elastin adherent to plastic that is capable of detecting 150 picograms of pancreatic elastase. Tritium 78-80 elastin Homo sapiens 102-109 7030400-2 1981 Monocyte cell lysates exhibited activity at neutral pH against azocasein, 3H-labelled elastin as well as several synthetic substrates used to detect serine proteinases (EC 3.4.21.-) of human polymorphonuclear leucocytes. Tritium 74-76 elastin Homo sapiens 86-93