PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15004651-8 2004 We also noticed a parallel increase in 3H-leucine incorporation when apoptosis induced by puromycin was lowered as an effect of IGF-1, in contrast to cycloheximide and emetine where IGF-1 reduced the apoptosis level without increasing the 3H-leucine incorporation. Tritium 39-41 insulin like growth factor 1 Homo sapiens 128-133 15178549-10 2004 IGFBP-3 also caused IGF-I-independent inhibition of basal [3H]thymidine incorporation. Tritium 59-61 insulin like growth factor 1 Homo sapiens 20-25 16094530-13 2005 IGF-I at 10(-9)-10(-8) mol/l significantly stimulated [3H]thymidine incorporation and at a concentration of 10(-9)-10(-7) mol/l also D-[U-14C]glucose accumulation in HCASMCs. Tritium 55-57 insulin like growth factor 1 Homo sapiens 0-5 16030168-13 2005 Stimulation of [3H]thymidine uptake was most responsive to IGF-I; insulin and glulisine were equally less effective, with sensitivities approximately 1-2% of that for IGF-I. Tritium 16-18 insulin like growth factor 1 Homo sapiens 59-64 15506488-4 2004 RESULTS: [3H] thymidine incorporation into TM cells was promoted by 10 and 100 ng/ml of PDGF, IGF-1, and FGF2 after 24 and 48 hours, whereas 1, 10, and 100 ng/ml of VEGF restrained cell proliferation after 48 hours. Tritium 10-12 insulin like growth factor 1 Homo sapiens 94-99 12572863-11 2002 An [3H]thymidine incorporation study demonstrated that platelet-derived growth factor (PDGF)-BB, basic fibroblast growth factor (bFGF), and insulin-like growth factor (IGF)-I significantly increased the uptake of [3H]thymidine into isolated SEMFs. Tritium 4-6 insulin like growth factor 1 Homo sapiens 140-174 12932745-5 2003 IGF-I was a weak mitogen, 1.5-fold increase in [3H]thymidine incorporation, for CoSMC. Tritium 48-50 insulin like growth factor 1 Homo sapiens 0-5 11342260-8 2001 There were significant increases in [3H]thymidine and [3H]leucine incorporation in IGF-I-treated cells in both 2 mM and 100 microM glutamine. Tritium 37-39 insulin like growth factor 1 Homo sapiens 83-88 12127559-3 2002 Abundant expression of IGF-I, IGF-II and IGF-IR was demonstrated in resting cells and exogenous IGF-I and IGF-II increased 3H-thymidine incorporation in a dose dependent manner. Tritium 123-125 insulin like growth factor 1 Homo sapiens 23-28 11768110-9 2001 Both IGF-I and bFGF exhibited dose-dependent increases in [3H]thymidine incorporation with increasing concentration from 1 to 50 ng/mL (P < 0.05), whereas TGF-beta1 caused a dose-dependent decrease from 0.01 to 0.5 ng/mL (P < 0.05). Tritium 59-61 insulin like growth factor 1 Homo sapiens 5-10 11342260-8 2001 There were significant increases in [3H]thymidine and [3H]leucine incorporation in IGF-I-treated cells in both 2 mM and 100 microM glutamine. Tritium 55-57 insulin like growth factor 1 Homo sapiens 83-88 10764740-3 2000 Treatment of cortical neurons with IGF-1 (100 ng/ml) for 30 min stimulates [(3)H]methionine incorporation, and a parallel increase in eIF-2B activity was observed. Tritium 75-81 insulin like growth factor 1 Homo sapiens 35-40 9655695-3 1998 Growth induced by a maximally effective concentration of IGF-I (100 nM), measured as [3H]thymidine incorporation, was only partially inhibited by LY-294002 [phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor] or PD-98059 [mitogen-activated protein (MAP) kinase kinase (MEK) inhibitor] (86 +/- 7% and 35 +/- 6% inhibition, respectively) alone but was abolished by the two combined (114 +/- 18% inhibition), implying the participation of both pathways. Tritium 86-88 insulin like growth factor 1 Homo sapiens 57-62 9886566-5 1998 Both IGF-I and -II stimulated [3H]-thymidine incorporation dose-dependently. Tritium 31-33 insulin like growth factor 1 Homo sapiens 5-18 9762008-8 1998 IGF-I and IGF-II (0.3-30 nM) dose-dependently stimulated [3H]thymidine incorporation, with a maximal response of 110% above basal. Tritium 58-60 insulin like growth factor 1 Homo sapiens 0-5 9654167-3 1998 RESULTS: IGF-I alone resulted in a significant dose-dependent increase in medium estradiol (E2) (p<0.05) and progesterone (P) (p<0.001) and suppression of IGF-binding protein-1 (IGFBP-1) (p<0.001), without any increase in [3H]-thymidine incorporation (P=0.10). Tritium 232-234 insulin like growth factor 1 Homo sapiens 9-14 9654167-4 1998 The combination of IGF-I and GH further increased the release of E2 (p<0.001), and the amount of [3H]-thymidine incorporation (p<0.001). Tritium 101-103 insulin like growth factor 1 Homo sapiens 19-24 9437509-4 1998 Glucose (49.5 mmol/L) attenuated the increment either in DNA content or in [3H]thymidine incorporation induced by insulin-like growth factor I (IGF-I). Tritium 76-78 insulin like growth factor 1 Homo sapiens 114-142 9437509-4 1998 Glucose (49.5 mmol/L) attenuated the increment either in DNA content or in [3H]thymidine incorporation induced by insulin-like growth factor I (IGF-I). Tritium 76-78 insulin like growth factor 1 Homo sapiens 144-149 9056422-4 1997 We report here that overexpression of the IGF-IR did not modify sensitivity of cells to IGF-I; however, responsiveness to the ligand was moderately enhanced in most of the MCF-7/IGF-IR clones (measured by [3H]thymidine incorporation into DNA). Tritium 206-208 insulin like growth factor 1 Homo sapiens 42-47 9264503-3 1997 Segments of the ascending aorta from the native and allograft hearts from 9 placebo-treated and 8 estradiol-treated recipients were compared by using IGF-I-stimulated [3H]thymidine incorporation. Tritium 168-170 insulin like growth factor 1 Homo sapiens 150-155 9292225-5 1997 Tranilast inhibited increases in [3H]-thymidine incorporation, DNA content and cell number induced by IGF-1. Tritium 34-36 insulin like growth factor 1 Homo sapiens 102-107 9430371-4 1997 Here we show that in human SMC exposed to individual growth factors (platelet-derived growth factor, epidermal growth factor, alpha-thrombin, insulin, insulin-like growth factor I (IGF-I)) and human serum, the maximal [3H]thymidine incorporation and c-fos mRNA expression are closely correlated. Tritium 219-221 insulin like growth factor 1 Homo sapiens 151-179 9430371-4 1997 Here we show that in human SMC exposed to individual growth factors (platelet-derived growth factor, epidermal growth factor, alpha-thrombin, insulin, insulin-like growth factor I (IGF-I)) and human serum, the maximal [3H]thymidine incorporation and c-fos mRNA expression are closely correlated. Tritium 219-221 insulin like growth factor 1 Homo sapiens 181-186 8869589-9 1996 IGFBP-4 proteolyzed in the conditioned medium from cells treated with PTH and 17 beta-estradiol was less effective at inhibiting IGF-I-stimulated [3H]thymidine incorporation into DNA compared with that proteolyzed in the conditioned medium from PTH-treated cells. Tritium 147-149 insulin like growth factor 1 Homo sapiens 129-134 8996625-8 1996 The most effective combination of growth factors, PDGF-BB (50 ng/ml) + bFGF(20 ng/ml) + IGF-1 (50 ng/ml), produced a 3H-thymidine incorporation of 44 +/- 10% (human restenosed) and 42 +/- 11% (minipig post angioplasty) of FCS values. Tritium 117-119 insulin like growth factor 1 Homo sapiens 88-93 9218734-6 1997 In the cells in culture, cancer cells showed increased incorporation of [35S]methionine into protein and [3H]thymidine into DNA (P < 0.02) when treated with IGF-I. Tritium 106-108 insulin like growth factor 1 Homo sapiens 160-165 8912808-6 1996 Human IGF-1 mimicked the effects of low PGE2 concentrations by stimulating in a dose-dependent (ANOVA, F= 31.65, P < 0.001, n = 3) and saturable fashion the incorporation of [3H]proline into CDP although the magnitude of the response induced by IGF-1 was far greater (3.5-fold). Tritium 178-181 insulin like growth factor 1 Homo sapiens 6-11 8912808-6 1996 Human IGF-1 mimicked the effects of low PGE2 concentrations by stimulating in a dose-dependent (ANOVA, F= 31.65, P < 0.001, n = 3) and saturable fashion the incorporation of [3H]proline into CDP although the magnitude of the response induced by IGF-1 was far greater (3.5-fold). Tritium 178-181 insulin like growth factor 1 Homo sapiens 248-253 8912808-8 1996 Furthermore, the PGE2-induced increase in [3H]proline incorporation into CDP was inhibited (63%, P < 0.001, n = 7) by the addition to the culture medium of an anti-IGF-1 antibody. Tritium 43-45 insulin like growth factor 1 Homo sapiens 167-172 7473836-16 1995 Incorporation of [3H]thymidine into DNA in SK-ES-1 (but not MNNG/HOS) cells was increased by 25 ng/mL IGF-I (P < .01). Tritium 18-20 insulin like growth factor 1 Homo sapiens 102-107 8660939-2 1996 Simultaneous addition of insulin-like growth factor 1 (IGF-1), itself also poorly mitogenic, led to a significant increase in [3H]thymidine incorporation into the cell DNA as well as a strong increase in cell number. Tritium 127-129 insulin like growth factor 1 Homo sapiens 25-53 8660939-2 1996 Simultaneous addition of insulin-like growth factor 1 (IGF-1), itself also poorly mitogenic, led to a significant increase in [3H]thymidine incorporation into the cell DNA as well as a strong increase in cell number. Tritium 127-129 insulin like growth factor 1 Homo sapiens 55-60 8769688-4 1996 Human recombinant IGF-I and IGF-II added to cSMC cultured in a medium containing 10% fetal bovine serum (FBS) decreased the cell number and [3H]thymidine incorporation in a dose dependent fashion up to 40% and 43% compared to control cells (100%). Tritium 141-143 insulin like growth factor 1 Homo sapiens 18-23 7543631-6 1995 FGF, IGF-1, and EGF were each capable of stimulating [3H]thy uptake on Day 1 of culture (2372 +/- 335 FGF, 2226 +/- 193 IGF-1, 4037 +/- 679 EGF vs 1108 +/- 70 MEM, P < 0.05). Tritium 54-56 insulin like growth factor 1 Homo sapiens 5-10 8562683-6 1995 Coincubation with IGF-I antibody also suppressed basal and IGF-I (10 ng/ml)-induced [3H]thymidine incorporation into GC DNA, but had no effect on insulin (1 microgram/ml)-induced DNA synthesis, demonstrating the specificity and lack of toxicity of the IGF-I antibody. Tritium 85-87 insulin like growth factor 1 Homo sapiens 18-23 8562683-6 1995 Coincubation with IGF-I antibody also suppressed basal and IGF-I (10 ng/ml)-induced [3H]thymidine incorporation into GC DNA, but had no effect on insulin (1 microgram/ml)-induced DNA synthesis, demonstrating the specificity and lack of toxicity of the IGF-I antibody. Tritium 85-87 insulin like growth factor 1 Homo sapiens 59-64 8562683-6 1995 Coincubation with IGF-I antibody also suppressed basal and IGF-I (10 ng/ml)-induced [3H]thymidine incorporation into GC DNA, but had no effect on insulin (1 microgram/ml)-induced DNA synthesis, demonstrating the specificity and lack of toxicity of the IGF-I antibody. Tritium 85-87 insulin like growth factor 1 Homo sapiens 59-64 8562683-5 1995 When IGF-I activity in the GC cultures was neutralized with a specific IGF-I antibody, relaxin (10 and 100 ng/ml)-induced [3H]thymidine incorporation was inhibited (p < 0.05). Tritium 123-125 insulin like growth factor 1 Homo sapiens 5-10 8562683-5 1995 When IGF-I activity in the GC cultures was neutralized with a specific IGF-I antibody, relaxin (10 and 100 ng/ml)-induced [3H]thymidine incorporation was inhibited (p < 0.05). Tritium 123-125 insulin like growth factor 1 Homo sapiens 71-76 7543631-7 1995 IGF-1 and EGF also stimulated [3H]thy uptake on Days 3 and 7 of culture. Tritium 31-33 insulin like growth factor 1 Homo sapiens 0-5 8048522-6 1994 IGF-I stimulated [3H]thymidine incorporation and cell proliferation in a dose-dependent manner with detectable effect observed with 0.5 nM IGF-I and maximal effect at 50 nM IGF-I. Tritium 18-20 insulin like growth factor 1 Homo sapiens 0-5 7541943-5 1995 However, the IGF-I-stimulated increase in [3H]AIB uptake was completely prevented by concurrent treatment with IGFBP-3. Tritium 43-45 insulin like growth factor 1 Homo sapiens 13-18 7734622-5 1995 IGF-I, forskolin and PMA each increased (P < 0.01) [3H]-thymidine incorporation in a dose and time dependent manner. Tritium 55-57 insulin like growth factor 1 Homo sapiens 0-5 7775603-4 1995 Half-maximal effectiveness was seen at 5-8 ng/ml IGF-I, with mean maximal responses of 1.5-, 2-, and 6-fold for [3H]2-deoxyglucose uptake, [14C]glucose incorporation, and [14C]aminoisobutyric acid (AIB) uptake, respectively. Tritium 113-115 insulin like growth factor 1 Homo sapiens 49-54 8817658-4 1995 30 nM IGFBP-5 stimulates 3H-thymidine incorporation in calvaria (which produce IGF I contributing to basal proliferation in serum-free medium) but not in Saos-2 cells which produce little IGF I and IGF II. Tritium 25-27 insulin like growth factor 1 Homo sapiens 79-84 8048522-6 1994 IGF-I stimulated [3H]thymidine incorporation and cell proliferation in a dose-dependent manner with detectable effect observed with 0.5 nM IGF-I and maximal effect at 50 nM IGF-I. Tritium 18-20 insulin like growth factor 1 Homo sapiens 139-144 8048522-6 1994 IGF-I stimulated [3H]thymidine incorporation and cell proliferation in a dose-dependent manner with detectable effect observed with 0.5 nM IGF-I and maximal effect at 50 nM IGF-I. Tritium 18-20 insulin like growth factor 1 Homo sapiens 139-144 8040840-9 1994 IGF-I, IGF-II and insulin each enhanced 2[3H]DG transport in a dose-dependent fashion. Tritium 42-44 insulin like growth factor 1 Homo sapiens 0-5 8188767-11 1994 The anti-IGF-I receptor antibody alpha IR3 blocked the IGF-I and IGF-II stimulated increase of 3H-thymidine uptake. Tritium 95-97 insulin like growth factor 1 Homo sapiens 9-14 7517700-6 1994 However, in the presence of 2% serum, which induces half-maximal stimulation, IGF-I (100 ng/ml) further increased the rate of 3H-thymidine incorporation in stromal but not smooth muscle cells (p < 0.05). Tritium 126-128 insulin like growth factor 1 Homo sapiens 78-83 7691836-10 1993 IGF-I alone (20 ng/ml) induced a 1.6 to threefold increase in 3H-thymidine incorporation. Tritium 62-64 insulin like growth factor 1 Homo sapiens 0-5 7512304-10 1994 However, pretreatment of cultured hOB with IGF-II enhanced IGF-I-stimulated [3H]thymidine incorporation threefold. Tritium 77-79 insulin like growth factor 1 Homo sapiens 43-48 8219478-2 1993 GH and IGF-I were mitogenic for chondrocytes and SaOS-2 cells, as indicated by the dose-related increase in uptake of [3H]thymidine. Tritium 119-121 insulin like growth factor 1 Homo sapiens 7-12 8409903-7 1993 Bovine insulin (10 micrograms/ml) and human recombinant insulin-like growth factor-I (IGF-I, 15 ng/ml) also doubled [3H]thymidine uptake in PrM cultures, but lower doses were less effective and estradiol-17 beta, transferrin, adult shark serum, purified shark relaxin, and a variety of other known growth factors were neither stimulatory nor inhibitory at the doses and conditions tested. Tritium 117-119 insulin like growth factor 1 Homo sapiens 56-84 8409903-7 1993 Bovine insulin (10 micrograms/ml) and human recombinant insulin-like growth factor-I (IGF-I, 15 ng/ml) also doubled [3H]thymidine uptake in PrM cultures, but lower doses were less effective and estradiol-17 beta, transferrin, adult shark serum, purified shark relaxin, and a variety of other known growth factors were neither stimulatory nor inhibitory at the doses and conditions tested. Tritium 117-119 insulin like growth factor 1 Homo sapiens 86-91 7682287-6 1993 The IGF-I AS-Oligo also inhibited [3H]thymidine incorporation into DNA in a dose-dependent fashion (by 77% at 2 microM and by 95% at 20 microM). Tritium 35-37 insulin like growth factor 1 Homo sapiens 4-9 8514622-10 1993 IGF-1, but not GH, had proliferative effects (significant increases in the rate of 3H-Thy incorporation, DNA, and protein content) but no effect on 5-alpha-R activity, Bmax or Kd. Tritium 83-85 insulin like growth factor 1 Homo sapiens 0-5 8488157-2 1993 Under steroid and growth factor-free culture conditions, IGF-I significantly stimulated the androgen-independent cell lines PC-3 and DU-145 to incorporate [3H]thymidine into DNA, while the androgen-dependent cell line, LNCaP, was not affected. Tritium 156-158 insulin like growth factor 1 Homo sapiens 57-62 1332906-1 1992 Insulin-like growth factor-I (IGF-I) stimulated the growth and [3H]thymidine uptake in MCF-7 human breast cancer cells grown in serum- and growth factor-inactivated serum-containing media. Tritium 64-66 insulin like growth factor 1 Homo sapiens 0-28 1332906-1 1992 Insulin-like growth factor-I (IGF-I) stimulated the growth and [3H]thymidine uptake in MCF-7 human breast cancer cells grown in serum- and growth factor-inactivated serum-containing media. Tritium 64-66 insulin like growth factor 1 Homo sapiens 30-35 1332906-2 1992 Cotreatment of the cells with IGF-I plus 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) resulted in a significant decrease in mitogen-induced cell proliferation and [3H]thymidine uptake. Tritium 162-164 insulin like growth factor 1 Homo sapiens 30-35 1322575-6 1992 In cells cotreated with TCDD plus 100 ng/ml insulin or 10 nM 17 beta-estradiol, EGF, TGF-alpha and IGF-I, TCDD caused a concentration-dependent decrease in cell proliferation and [3H]thymidine uptake. Tritium 180-182 insulin like growth factor 1 Homo sapiens 99-104 1325119-6 1992 IGF-I (100 pM-100 nM) stimulated the incorporation of radiolabeled alanine as a measure for total protein synthesis, 3H-labeled thymidine incorporation into DNA, and glycogen synthesis at 20 and 1% O2 in a concentration-dependent fashion. Tritium 117-119 insulin like growth factor 1 Homo sapiens 0-5 1323990-2 1992 The 125I-IGF1 binding was performed on frozen tissue sections and analysed using 3H Ultrofilm autoradiography coupled to computerised image analysis. Tritium 81-83 insulin like growth factor 1 Homo sapiens 9-13 1396882-8 1992 IGF-I from the patient showed normal bioactivities when determined by [35S]sulphate and [3H]thymidine uptake into cultured rat chondrocytes, and by [3H]thymidine and [3H]alpha-aminoisobutyric acid uptake into the patient"s skin fibroblasts. Tritium 89-91 insulin like growth factor 1 Homo sapiens 0-5 1396882-8 1992 IGF-I from the patient showed normal bioactivities when determined by [35S]sulphate and [3H]thymidine uptake into cultured rat chondrocytes, and by [3H]thymidine and [3H]alpha-aminoisobutyric acid uptake into the patient"s skin fibroblasts. Tritium 149-151 insulin like growth factor 1 Homo sapiens 0-5 1396882-8 1992 IGF-I from the patient showed normal bioactivities when determined by [35S]sulphate and [3H]thymidine uptake into cultured rat chondrocytes, and by [3H]thymidine and [3H]alpha-aminoisobutyric acid uptake into the patient"s skin fibroblasts. Tritium 149-151 insulin like growth factor 1 Homo sapiens 0-5 1936596-12 1991 However, IGF-I decreased the ratio of [35S]sulfate to [3H]glucosamine in proteoglycans and their glycosaminoglycan side chains. Tritium 55-57 insulin like growth factor 1 Homo sapiens 9-14 1823034-9 1991 We also measured other growth-blocking IgG that inhibited [3H]-thymidine incorporation into DNA stimulated by insulin-like growth factor-I (IGF-I), a growth factor working through a cAMP-independent pathway. Tritium 59-61 insulin like growth factor 1 Homo sapiens 110-138 1823034-9 1991 We also measured other growth-blocking IgG that inhibited [3H]-thymidine incorporation into DNA stimulated by insulin-like growth factor-I (IGF-I), a growth factor working through a cAMP-independent pathway. Tritium 59-61 insulin like growth factor 1 Homo sapiens 140-145 1613431-10 1992 Pretreatment of cells with GH or IGF-I for 2.5 days showed that GH but not IGF-I produced a sustained effect on [3H]thymidine uptake. Tritium 113-115 insulin like growth factor 1 Homo sapiens 33-38 1311720-12 1992 In MCF-7 cells both IGF-I and insulin stimulated [3H]thymidine incorporation; alpha-IR3 blocked all of the IGF-I effect but only 50-60% of the insulin effect. Tritium 50-52 insulin like growth factor 1 Homo sapiens 20-25 1483875-4 1992 Exogenous IGF-I (20 ng/mL) stimulated [3H]thymidine incorporation in serum-free medium; approximately 70% of the IGF-I-induced stimulation was blocked by the presence of the receptor antibody. Tritium 39-41 insulin like growth factor 1 Homo sapiens 10-15 1483875-4 1992 Exogenous IGF-I (20 ng/mL) stimulated [3H]thymidine incorporation in serum-free medium; approximately 70% of the IGF-I-induced stimulation was blocked by the presence of the receptor antibody. Tritium 39-41 insulin like growth factor 1 Homo sapiens 113-118 1752778-3 1991 It was concluded that the tumor content of IGF-1 and the proportion of IGF-I-positive cells were inversely correlated to the tumor growth and the 3H-thymidine labeling index in vivo. Tritium 146-148 insulin like growth factor 1 Homo sapiens 43-48 1752778-3 1991 It was concluded that the tumor content of IGF-1 and the proportion of IGF-I-positive cells were inversely correlated to the tumor growth and the 3H-thymidine labeling index in vivo. Tritium 146-148 insulin like growth factor 1 Homo sapiens 71-76 2607258-7 1989 The growth of human thyroid epithelial cells was also found to be increased by IGF-I (25-100 ng/ml) added in medium plus 1% fetal calf serum as assessed by the incorporation of [3H]thymidine into DNA. Tritium 178-180 insulin like growth factor 1 Homo sapiens 79-84 1903729-4 1991 The ability of hCG, human FSH, and/or IGF-I to induce aromatase activity was assayed by the stereospecific release of tritium from [1B-3H]androstenedione. Tritium 118-125 insulin like growth factor 1 Homo sapiens 38-43 2175183-4 1990 In monolayer cultures of fetal skin fibroblasts both forms of IGFBP-1 inhibited binding of [125I]IGF-I onto the cells, but amplified the IGF-I-stimulated [3H]thymidine incorporation into the same cells. Tritium 155-157 insulin like growth factor 1 Homo sapiens 137-142 2175183-7 1990 Recently we found that the same IGFBP-1 preparation inhibits IGF-I-stimulated [3H]thymidine incorporation into human hyperstimulated granulosa cells. Tritium 79-81 insulin like growth factor 1 Homo sapiens 61-66 2390866-5 1990 It had a 50% potency compared to human IGF-I in a radioimmunoassay using an antiserum raised against human IGF-I, stimulated the incorporation of [3H]-thymidine into DNA in cultured chick embryo myoblasts with a half-maximum effective dose of 5 ng/ml and displaced [125I]-labelled human IGF-I and IGF-II from binding sites in microsomal membranes prepared from both the chicken liver and the lactating rabbit mammary gland in a dose dependent manner. Tritium 147-149 insulin like growth factor 1 Homo sapiens 39-44 2390866-5 1990 It had a 50% potency compared to human IGF-I in a radioimmunoassay using an antiserum raised against human IGF-I, stimulated the incorporation of [3H]-thymidine into DNA in cultured chick embryo myoblasts with a half-maximum effective dose of 5 ng/ml and displaced [125I]-labelled human IGF-I and IGF-II from binding sites in microsomal membranes prepared from both the chicken liver and the lactating rabbit mammary gland in a dose dependent manner. Tritium 147-149 insulin like growth factor 1 Homo sapiens 107-112 2390866-5 1990 It had a 50% potency compared to human IGF-I in a radioimmunoassay using an antiserum raised against human IGF-I, stimulated the incorporation of [3H]-thymidine into DNA in cultured chick embryo myoblasts with a half-maximum effective dose of 5 ng/ml and displaced [125I]-labelled human IGF-I and IGF-II from binding sites in microsomal membranes prepared from both the chicken liver and the lactating rabbit mammary gland in a dose dependent manner. Tritium 147-149 insulin like growth factor 1 Homo sapiens 107-112 2156621-6 1990 In both cell lines [3H]thymidine incorporation was enhanced by recombinant human IGF-I, 100-1000 ng/ml. Tritium 20-22 insulin like growth factor 1 Homo sapiens 81-86 2156621-13 1990 All seven samples showed stimulation of [3H]thymidine incorporation in the presence of recombinant human IGF-I, 100-500 ng/ml. Tritium 41-43 insulin like growth factor 1 Homo sapiens 105-110 1717510-8 1991 Changes in cell surface receptor number were reflected in cell responsiveness to IGF-I-stimulated [3H]thymidine incorporation and [3H]aminoisobutyric acid uptake. Tritium 99-101 insulin like growth factor 1 Homo sapiens 81-86 1721920-2 1991 Physiological concentrations of IGF-I (2-200 ng/ml) were found to stimulate [3H]thymidine incorporation into the cells in a concentration-dependent manner. Tritium 77-79 insulin like growth factor 1 Homo sapiens 32-37 1721920-3 1991 Half-maximal stimulation of [3H]thymidine incorporation was obtained with 10 ng/ml exogenous IGF-I, which was chosen for suppression experiments with graded amounts of purified IGFBP-1. Tritium 29-31 insulin like growth factor 1 Homo sapiens 93-98 2156796-6 1990 IGF-1, insulin, and EGF stimulated [3H]thymidine incorporation by 77, 73, and 87%, respectively. Tritium 36-38 insulin like growth factor 1 Homo sapiens 0-5 3508726-0 1986 A recombinant IGF-1 analogue stimulates [3H]thymidine incorporation in human epiphyseal growth cartilage in vitro. Tritium 41-43 insulin like growth factor 1 Homo sapiens 14-19 3508726-2 1986 Addition of 100 ng/ml IGF-1 to articular and epiphyseal growth plate cartilage (9 months to 16 years) resulted in an increase in [3H]thymidine incorporation to a mean of 130 +/- 4% (SE) and 378 +/- 53% of controls, respectively. Tritium 130-132 insulin like growth factor 1 Homo sapiens 22-27 3508726-3 1986 In costal and growth cartilage from normal fetuses of 15-18 weeks gestation, [3H]thymidine incorporation was increased to 119 +/- 3 and 133 +/- 11% of controls, respectively, when 100 ng/ml IGF-1 was added. Tritium 78-80 insulin like growth factor 1 Homo sapiens 190-195 2607258-8 1989 In the presence of a monoclonal antibody to IGF-I the increase in [3H]thymidine uptake in response to IGF-I was abolished as was that seen in response to TSH. Tritium 67-69 insulin like growth factor 1 Homo sapiens 44-49 2607258-8 1989 In the presence of a monoclonal antibody to IGF-I the increase in [3H]thymidine uptake in response to IGF-I was abolished as was that seen in response to TSH. Tritium 67-69 insulin like growth factor 1 Homo sapiens 102-107 2530275-3 1989 MGF, pTGF-beta 1, and pTGF-beta 2 decreased mitogen-induced [3H]thymidine incorporation by 30 to 75% in a dose-dependent manner. Tritium 61-63 insulin like growth factor 1 Homo sapiens 0-3 2530275-10 1989 Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. Tritium 77-79 insulin like growth factor 1 Homo sapiens 13-16 2530275-10 1989 Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. Tritium 155-157 insulin like growth factor 1 Homo sapiens 13-16 2546962-6 1989 Thus, [125I]IGF binding was approximately 50%, IGF-I stimulated [3H]thymidine uptake was approximately 40% and 60% of the control value, and IGF-I-stimulated receptor autophosphorylation was reduced by 40%. Tritium 65-67 insulin like growth factor 1 Homo sapiens 47-52 2803252-0 1989 Insulin-like effects of epidermal growth factor and insulin-like growth factor-I on [3H]2-deoxyglucose uptake, diacylglycerol generation and protein kinase C activation in BC3H-1 myocytes. Tritium 85-87 insulin like growth factor 1 Homo sapiens 52-80 2803252-1 1989 Epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) were found to provoke increases in [3H]2-deoxyglucose uptake, diacylglycerol (DAG) generation and membrane-bound protein kinase C activity in BC3H-1 myocytes. Tritium 107-109 insulin like growth factor 1 Homo sapiens 34-62 2803252-1 1989 Epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) were found to provoke increases in [3H]2-deoxyglucose uptake, diacylglycerol (DAG) generation and membrane-bound protein kinase C activity in BC3H-1 myocytes. Tritium 107-109 insulin like growth factor 1 Homo sapiens 64-69 2803252-4 1989 Accordingly, both EGF and IGF-I increased acute [3H]glycerol labelling of DAG (and other lipids) and [3H]choline labelling of phosphocholine. Tritium 49-51 insulin like growth factor 1 Homo sapiens 26-31 2803252-4 1989 Accordingly, both EGF and IGF-I increased acute [3H]glycerol labelling of DAG (and other lipids) and [3H]choline labelling of phosphocholine. Tritium 102-104 insulin like growth factor 1 Homo sapiens 26-31 2693820-0 1989 Insulin and insulin-like growth factor (IGF I) modulate the effects of bTSH on 3H-thymidine incorporation in human thyroid cells in primary culture. Tritium 79-81 insulin like growth factor 1 Homo sapiens 40-45 2693820-3 1989 In the present study, we have therefore undertaken to elucidate the effects of insulin and insulin-like growth factor (IGF I) on 3H-thymidine uptake in human thyroid cells, particularly with respect to their relation to the actions of bTSH. Tritium 129-131 insulin like growth factor 1 Homo sapiens 119-124 2693820-4 1989 We could demonstrate a considerable, dose-dependent stimulation of 3H-thymidine incorporation in the cells by bTSH that was dependent on the presence of insulin or IGF I; bTSH alone was ineffective in that respect. Tritium 67-69 insulin like growth factor 1 Homo sapiens 164-169 3654910-9 1987 The IGF-I components isolated by HPLC were normally active in stimulating [3H] alpha-aminoisobutyric acid uptake by normal human fibroblasts. Tritium 75-77 insulin like growth factor 1 Homo sapiens 4-9 2464943-7 1989 Examination of the effect of IGF-1 on the cell cycle revealed that exposure of cells to both IGF-1 and platelet-derived growth factor (PDGF) led to a significant increase in 3H-thymidine incorporation into cell layers. Tritium 174-176 insulin like growth factor 1 Homo sapiens 29-34 2464943-7 1989 Examination of the effect of IGF-1 on the cell cycle revealed that exposure of cells to both IGF-1 and platelet-derived growth factor (PDGF) led to a significant increase in 3H-thymidine incorporation into cell layers. Tritium 174-176 insulin like growth factor 1 Homo sapiens 93-98 2971653-10 1988 Concentrations of peak C material between 2 and 20 ng/ml inhibited IGF-I stimulation of [3H]thymidine incorporation into smooth muscle cell DNA. Tritium 89-91 insulin like growth factor 1 Homo sapiens 67-72 2837402-6 1988 The incorporation of [3H]thymidine in the presence of recombinant human IGF-I resulted in a clearly increased DNA synthesis in two of seven cell lines. Tritium 22-24 insulin like growth factor 1 Homo sapiens 72-77 2961773-7 1987 Similar results were obtained when IGF-I and IGF-II stimulation of [3H]thymidine incorporation was compared in fetal and postnatal fibroblasts. Tritium 68-70 insulin like growth factor 1 Homo sapiens 35-40 2961773-8 1987 Incubation in the presence of alpha IR-3, a monoclonal antibody to the type I IGF receptor, inhibited the ability of both IGF-I and IGF-II to stimulate [14C]AIB uptake and [3H]thymidine incorporation in fetal and postnatal cells. Tritium 173-175 insulin like growth factor 1 Homo sapiens 122-127 2661519-4 1989 However, a 48-h preincubation with dexamethasone resulted in a marked synergistic increase in IGF-I stimulation of [14C]aminoisobutyric acid uptake and [3H]thymidine incorporation in these cultures. Tritium 153-155 insulin like growth factor 1 Homo sapiens 94-99 3058540-11 1988 When the media were supplemented with growth factors, measurable increases in uptake of [3H]thymidine occurred with human epidermal growth factor (h-EGF), insulin-like growth factor-1 (IGF-1), nerve growth factor (NGF), transforming growth factor-beta (TGF-beta), bovine acidic fibroblast growth factor (baFGF), IL-1, bovine basic fibroblast growth factor (bbFGF), and Forskolin. Tritium 89-91 insulin like growth factor 1 Homo sapiens 155-183 3058540-11 1988 When the media were supplemented with growth factors, measurable increases in uptake of [3H]thymidine occurred with human epidermal growth factor (h-EGF), insulin-like growth factor-1 (IGF-1), nerve growth factor (NGF), transforming growth factor-beta (TGF-beta), bovine acidic fibroblast growth factor (baFGF), IL-1, bovine basic fibroblast growth factor (bbFGF), and Forskolin. Tritium 89-91 insulin like growth factor 1 Homo sapiens 185-190 3003744-2 1986 Although insulin and IGF-I are known to stimulate the incorporation of [3H]thymidine into DNA in these cells, the identity of the receptor(s) that mediates this effect has not been fully clarified. Tritium 72-74 insulin like growth factor 1 Homo sapiens 21-26 3498926-3 1987 In both DF and control cells, SM-C/IGF-I (50 ng/ml), insulin (100 ng/ml), and epidermal growth factor (5 ng/ml) stimulated [3H]thymidine incorporation 5-, 2-, and 6-fold, respectively. Tritium 124-126 insulin like growth factor 1 Homo sapiens 35-40 3498926-6 1987 Preincubation of control cells with dexamethasone (10(-7) M) caused a marked synergistic increase in SM-C/IGF-I stimulated [3H]thymidine incorporation (15- to 20-fold in serum-free medium; 50- to 80-fold in 0.25% human hypopituitary serum). Tritium 124-126 insulin like growth factor 1 Homo sapiens 106-111 3009492-1 1986 Human insulin-like growth factor I and II (IGF-I and IGF-II) in concentrations of 1-30 ng/ml, were shown to stimulate ornithine decarboxylase activity and [3H]thymidine incorporation in human SH-SY5Y neuroblastoma cells. Tritium 156-158 insulin like growth factor 1 Homo sapiens 6-41 3009492-1 1986 Human insulin-like growth factor I and II (IGF-I and IGF-II) in concentrations of 1-30 ng/ml, were shown to stimulate ornithine decarboxylase activity and [3H]thymidine incorporation in human SH-SY5Y neuroblastoma cells. Tritium 156-158 insulin like growth factor 1 Homo sapiens 43-48 3571867-3 1987 Maximal concentrations of SM-C/IGF-I (100 ng/ml) stimulated [3H]thymidine incorporation equally in both early and late passage cells. Tritium 61-63 insulin like growth factor 1 Homo sapiens 31-36 2945555-4 1986 However, alpha IR-3 inhibited both insulin-like growth factor I and II stimulated [3H]thymidine incorporation. Tritium 83-85 insulin like growth factor 1 Homo sapiens 35-63 2873626-2 1986 Combinations of palytoxin or TPA with recombinant human IGF-I or insulin also synergistically stimulate deesterification of cellular lipids in C-9 cells prelabelled with [3H]arachidonic acid. Tritium 171-173 insulin like growth factor 1 Homo sapiens 56-61 3003744-4 1986 alpha IR-3 competitively inhibits IGF-I-mediated stimulation of [3H]thymidine incorporation into DNA. Tritium 65-67 insulin like growth factor 1 Homo sapiens 34-39 3967052-3 1985 The 120% increase in radiolabeling of collagen with [3H]proline by 10 nM somatomedin C was not due to changes in the rate of [3H]proline uptake. Tritium 53-55 insulin like growth factor 1 Homo sapiens 73-86 4040529-1 1985 The effects of dexamethasone on somatomedin-C (SM-C) stimulation of [3H]thymidine incorporation and cell replication were studied in early passage fibroblasts from normal donors, aged 7-24 yr (young) and 85-96 yr (old), and one patient with progeria. Tritium 69-71 insulin like growth factor 1 Homo sapiens 32-45 3881885-7 1985 The rise of endogenous rat IGF as well as the infused human IGF I and II caused a widening of the tibial epiphysis and an increase of the [3H]thymidine incorporation into costal cartilage. Tritium 139-141 insulin like growth factor 1 Homo sapiens 60-72 3880762-1 1985 At concentrations of 25 ng/ml in serum-free medium, somatomedin C (SM-C) and insulin stimulated 3H-thymidine incorporation in adult human fibroblasts 4- and 1.5-fold, respectively. Tritium 96-98 insulin like growth factor 1 Homo sapiens 52-65 6386833-5 1984 Somatomedin-C (Sm-C), in contrast, was found to stimulate a 320% increase in [3H]thymidine incorporation when concentrations that are present in extracellular fluids were used (i.e., 0.5-10 ng/ml). Tritium 78-80 insulin like growth factor 1 Homo sapiens 0-13 6362310-3 1983 We have measured the binding of [125I]SM-C/IGF-I to cultured fibroblast monolayers derived from patients with Turner syndrome, and have evaluated SM-C/IGF-I stimulation of both [3H]thymidine incorporation and cell replication. Tritium 178-180 insulin like growth factor 1 Homo sapiens 151-156 6090502-9 1984 Both IGF-I and IGF-II stimulated [3H]glucose incorporation over the range of 10 pM-10 nM; IGF-I was three to fivefold more potent. Tritium 34-36 insulin like growth factor 1 Homo sapiens 5-10 6090502-9 1984 Both IGF-I and IGF-II stimulated [3H]glucose incorporation over the range of 10 pM-10 nM; IGF-I was three to fivefold more potent. Tritium 34-36 insulin like growth factor 1 Homo sapiens 15-20 6362310-5 1983 Similarly, equivalent SM-C/IGF-I stimulation of [3H]thymidine incorporation was seen in both Turner and control fibroblasts. Tritium 49-51 insulin like growth factor 1 Homo sapiens 27-32 20924562-8 2010 Insulin-induced stimulation of [(3)H]-thymidine incorporation was reduced by 83% after siRNA-mediated down-regulation of IGF-1 receptor by about 75%, but not affected by a similar down-regulation of the insulin receptor. Tritium 31-36 insulin like growth factor 1 Homo sapiens 121-126