PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11278296-3 2001 Lymphocytes induced to proliferate with either the mitogen concanavalin A or with anti-CD3 (OKT3) in combination with interleukin 2 (OKT3/IL-2) showed a greatly accelerated rate of [3H]arachidonate-phospholipid remodeling compared with resting lymphocytes or with lymphocytes stimulated with OKT3 or IL-2 alone. Tritium 182-184 interleukin 2 Homo sapiens 118-131 20797845-9 2010 Pharmacokinetics of L19-IL2 was dose proportional over the tested range, with a terminal half-life of 2-3h. Tritium 104-106 interleukin 2 Homo sapiens 24-27 17725664-6 2007 IL-2 activity in culture supernatants was determined using the incorporation of 3H-TdR by the IL-2-dependent cell line CTLL and evaluated by ELISA. Tritium 80-82 interleukin 2 Homo sapiens 0-4 21180073-2 2002 METHODS: Immunocytochemistry and flow cytometric analysis were used to investigate the expression of interleukin-2 receptors (IL-2Rs) by using of specific IL-2R polyclonal antibody; MTT method and 3H-TdR incorporation method were used to examine the changes of proliferation of MCF-7 cells. Tritium 197-199 interleukin 2 Homo sapiens 101-114 11278296-3 2001 Lymphocytes induced to proliferate with either the mitogen concanavalin A or with anti-CD3 (OKT3) in combination with interleukin 2 (OKT3/IL-2) showed a greatly accelerated rate of [3H]arachidonate-phospholipid remodeling compared with resting lymphocytes or with lymphocytes stimulated with OKT3 or IL-2 alone. Tritium 182-184 interleukin 2 Homo sapiens 138-142 11278296-3 2001 Lymphocytes induced to proliferate with either the mitogen concanavalin A or with anti-CD3 (OKT3) in combination with interleukin 2 (OKT3/IL-2) showed a greatly accelerated rate of [3H]arachidonate-phospholipid remodeling compared with resting lymphocytes or with lymphocytes stimulated with OKT3 or IL-2 alone. Tritium 182-184 interleukin 2 Homo sapiens 300-304 10684036-2 1997 The responses to recombinant human IL-2 (rhIL-2) of these leukemic cells expressing IL-2R mRNA from 8 cases were assayed by 3H-thymidine incorporation. Tritium 124-126 interleukin 2 Homo sapiens 35-39 11435749-7 2001 The effect of dopamine on IL-2-induced cell proliferation in vitro was evaluated by [3H]thymidine incorporation and cytotoxicity by 51Cr release, receptors by radioligand binding, cAMP by an assay kit and apoptosis by DNA fragmentation. Tritium 85-87 interleukin 2 Homo sapiens 26-30 10806689-10 1998 SKOV3/IL-2 cell proliferate more slowly, the doubling time prolonged from 38.40 hr to 65.36 hr, 3H-uptaking decreased 52.3% of the parent cell. Tritium 96-98 interleukin 2 Homo sapiens 6-10 9776586-0 1998 Optimization and comparison of the MTT assay and the 3H-TdR assay for the detection of IL-2 in helper T cell precursor assays. Tritium 53-55 interleukin 2 Homo sapiens 87-91 9776586-5 1998 Due to the different measurement procedures, the volumes of the IL-2 dilutions were 75 microl in assays with MTT and 150 microl in assays with 3H-TdR. Tritium 143-145 interleukin 2 Homo sapiens 64-68 9776586-9 1998 Because of the possibility of IL-2 detection in the whole culture volume (150 microl), we found that the 3H-TdR assay was superior to the MTT assay with a 10-fold better sensitivity in different human sera. Tritium 105-107 interleukin 2 Homo sapiens 30-34 10778438-1 2000 Increasing of 3H-thymidine incorporation in lymphocytes of human peripheral blood which depends non-linearly on X-ray dose (3 cGy max) and interleukin-2 (IL-2) concentration (17.5 Units/ml) is shown. Tritium 14-16 interleukin 2 Homo sapiens 139-152 10778438-1 2000 Increasing of 3H-thymidine incorporation in lymphocytes of human peripheral blood which depends non-linearly on X-ray dose (3 cGy max) and interleukin-2 (IL-2) concentration (17.5 Units/ml) is shown. Tritium 14-16 interleukin 2 Homo sapiens 154-158 9879838-11 1998 In addition, IL-2-mediated [3H]-thymidine incorporation by MNC in patients with BD was significantly decreased. Tritium 28-30 interleukin 2 Homo sapiens 13-17 9839937-3 1998 IL-2 is detected by 3H-thymidine (3H-TdR) incorporation into the IL-2 dependent cell line, CTLL-2. Tritium 20-22 interleukin 2 Homo sapiens 0-4 9839937-3 1998 IL-2 is detected by 3H-thymidine (3H-TdR) incorporation into the IL-2 dependent cell line, CTLL-2. Tritium 20-22 interleukin 2 Homo sapiens 65-69 8968107-9 1996 Exogenous IL-10, IL-6, IL-2, and TNF-alpha significantly enhanced the [3H]thymidine uptake in 13 of 13 (100%), 5 of 13 (38%), 9 of 13 (69%), and 2 of 10 (20%) PTCPs costimulated with anti-CD40, respectively. Tritium 71-73 interleukin 2 Homo sapiens 23-27 7732026-6 1995 Induction of p56lck kinase activity correlated with T-cell-mediated cytotoxicity, whereas interleukin 2 secretion correlated with [3H]thymidine incorporation and proliferation. Tritium 131-133 interleukin 2 Homo sapiens 90-103 7671316-3 1995 In short-term cultures (up to 3 days), TGF-beta 1-S-ODNs-treated, interleukin 2-activated PBMCs displayed a growth advantage (up to 148%) compared to nonsense or untreated controls as measured by cell counting and [3H]thymidine incorporation. Tritium 215-217 interleukin 2 Homo sapiens 66-79 9024505-6 1996 In contrast, despite the fact that IL-7 inhibited the proliferation of CD3-AK cells cultured in IL-2, as determined by [3H]thymidine uptake, the recovery of viable cells was not reduced. Tritium 120-122 interleukin 2 Homo sapiens 96-100 7877187-3 1994 The expression efficiency of IL-2 cDNA in E. coli transformed with different plasmids was evaluated by means of SDS-PAGE and measuring 3H-TdR incorporation of IL-2-dependent activated T lymphocytes in the presence of bacterial extracts. Tritium 135-137 interleukin 2 Homo sapiens 29-33 8258328-7 1993 Proliferation of T cells ([3H]thymidine incorporation) following exposure to IL-2 was substantially reduced when the c-raf-1 AS oligodeoxyribonucleotide was added to cultures, while the mitogenic response to this factor remained almost unaffected in the presence of S and NS oligodeoxyribonucleotides. Tritium 27-29 interleukin 2 Homo sapiens 77-81 1859888-6 1991 Combinations of IL-7 with IL-2 and tumor necrosis factor-alpha showed an additive effect on [3H]TdR incorporation. Tritium 93-95 interleukin 2 Homo sapiens 26-62 8264344-4 1993 The major finding was that occurrence of significant inhibition of [3H]-thymidine incorporation in interleukin-2 (IL-2) expanded PBL after exposure with 4-OOH-IF was accompanied by substantial depletion of intracellular GSH content in these cells. Tritium 68-70 interleukin 2 Homo sapiens 99-112 8264344-4 1993 The major finding was that occurrence of significant inhibition of [3H]-thymidine incorporation in interleukin-2 (IL-2) expanded PBL after exposure with 4-OOH-IF was accompanied by substantial depletion of intracellular GSH content in these cells. Tritium 68-70 interleukin 2 Homo sapiens 114-118 1391948-3 1992 MMC as well as PBMC samples demonstrated significant synergy between IL-2 and anti-CD3 in the promotion of proliferation as measured by 3H thymidine incorporation on day 5 (P less than .001) or fold increase in cell number on day 14. Tritium 136-138 interleukin 2 Homo sapiens 69-73 1316551-2 1992 We report here that addition to the cultures of Bt2cAMP, a cell permeant analogue of cAMP, results in inhibition of IL-2-dependent proliferation, as assessed by [3H]Thymidine incorporation, in both NK and T cell clones. Tritium 162-164 interleukin 2 Homo sapiens 116-120 1721896-5 1991 Our findings showed that after resting for 48 hours without IL-2, IL-2-dependent cells reverted to small lymphocytes, ceased to incorporate 3H-TdR, and had no mRNA for activation antigens such as Tac or IL-2. Tritium 140-142 interleukin 2 Homo sapiens 66-70 1721896-5 1991 Our findings showed that after resting for 48 hours without IL-2, IL-2-dependent cells reverted to small lymphocytes, ceased to incorporate 3H-TdR, and had no mRNA for activation antigens such as Tac or IL-2. Tritium 140-142 interleukin 2 Homo sapiens 66-70 8445249-2 1993 This approach has been used to analyze dose-response curves (IL-2 concentration vs. level of proliferation) measured by MTT and [3H]TdR assays. Tritium 129-131 interleukin 2 Homo sapiens 61-65 8445249-3 1993 The values of functional dissociation constants, equivalent to IL-2 concentrations giving 50% of the maximal response, depended on the cell concentration and increased from 4 to 60 pM for the [3H]TdR assay and from 40 to 140 pM for the MTT assay when the cell concentration was increased from 2 x 10(3) to 4 x 10(4) cells/well. Tritium 193-195 interleukin 2 Homo sapiens 63-67 1766257-5 1991 With IL-2, 3H-TdR incorporation increased in cells from 5 out of 10 T-ALL patients, including those with CD7+4-8-, CD5+, and CD1+ acute leukemia. Tritium 11-13 interleukin 2 Homo sapiens 5-9 1786993-2 1991 Increased tritiated-thymidine [( 3H]-TdR) uptake was detected among pharyngeal cells incubated with recombinant human interleukin-2 (IL-2), and phytohaemagglutinin-P (PHA-P). Tritium 33-35 interleukin 2 Homo sapiens 118-131 1786993-2 1991 Increased tritiated-thymidine [( 3H]-TdR) uptake was detected among pharyngeal cells incubated with recombinant human interleukin-2 (IL-2), and phytohaemagglutinin-P (PHA-P). Tritium 33-35 interleukin 2 Homo sapiens 133-137 1777232-3 1991 The results showed that IL-2 stimulated [3H]-thymidine incorporation significantly in blasts of 8 out of 23 cases of AML. Tritium 41-43 interleukin 2 Homo sapiens 24-28 1828010-10 1991 Furthermore, "nonresponsiveness" of a T cell clones in a [3H]TdR assay to either IL-2 or IL-4 is not a stable feature since clones, unresponsive to a particular lymphokine, could be adapted to become responsive. Tritium 58-60 interleukin 2 Homo sapiens 81-85 1890018-3 1991 Our results indicated that antibodies directed against either DR, DQ, or DP were able to decrease 3H-TdR uptake if the cells had been activated by interleukin-2 (IL-2). Tritium 98-100 interleukin 2 Homo sapiens 147-160 1890018-3 1991 Our results indicated that antibodies directed against either DR, DQ, or DP were able to decrease 3H-TdR uptake if the cells had been activated by interleukin-2 (IL-2). Tritium 98-100 interleukin 2 Homo sapiens 162-166 1705511-2 1991 CD5+ cells incorporated [3H]thymidine more efficiently than unfractionated cells when stimulated with SAC and their response was augmented by the addition of interleukin (IL) 2 to the cultures. Tritium 25-27 interleukin 2 Homo sapiens 158-176 2789326-6 1989 We conclude that 3H-thymidine incorporation by malignant B cells such as HCL and B-NHL stimulated with mitogens and IL-2 will in most cases wrongly be attributed to proliferation by the B cells themselves, and instead has to be ascribed to contaminating T cells. Tritium 17-19 interleukin 2 Homo sapiens 116-120 2395860-4 1990 Furthermore, cell proliferation ([3H]thymidine incorporation) was stimulated by IL-2. Tritium 34-36 interleukin 2 Homo sapiens 80-84 2301012-2 1990 However, prior to target cell death very low concentrations of rIL-2 and IL-2 toxin synergistically stimulate [3H] thymidine incorporation despite inhibition of [14C] leucine uptake. Tritium 111-113 interleukin 2 Homo sapiens 64-68 2301012-3 1990 A sequential analysis of [3H] thymidine incorporation shows that high IL-2 toxin concentration (10(-9)-10(-7) M) stimulates DNA synthesis at 18 hr of culture and inhibits [3H] thymidine uptake after 24 hr, while low concentrations of IL-2 toxin (10(-12)-10(-10) M) exhibits stimulatory effects only after 24 hr of culture. Tritium 26-28 interleukin 2 Homo sapiens 70-74 2530275-10 1989 Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. Tritium 77-79 interleukin 2 Homo sapiens 62-66 2084826-8 1990 The modified 3H-TdR release assay is well-suited to monitor the immunological effects of immunotherapy, using IL-2 and LAK cells. Tritium 13-15 interleukin 2 Homo sapiens 110-114 2389115-4 1990 However, IL-2 had a pronounced influence on the [3H]thymidine incorporation in response to various stimuli 4-8 days after intradermal IL-2. Tritium 49-51 interleukin 2 Homo sapiens 9-13 2389115-4 1990 However, IL-2 had a pronounced influence on the [3H]thymidine incorporation in response to various stimuli 4-8 days after intradermal IL-2. Tritium 49-51 interleukin 2 Homo sapiens 134-138 2376610-10 1990 And the proliferation of PBMC after IL2 incubation, which was measured by incorporation of [3H]TdR, was reduced dose-dependently by M-PSL. Tritium 92-94 interleukin 2 Homo sapiens 36-39 2375921-3 1990 Although IL2 induced a proliferative signal in three of the 31 acute leukaemias analysed, the overall 3H-thymidine uptake of the neoplastic cells was significantly reduced (P less than 0.05) in the presence of IL2. Tritium 102-104 interleukin 2 Homo sapiens 210-213 2301012-3 1990 A sequential analysis of [3H] thymidine incorporation shows that high IL-2 toxin concentration (10(-9)-10(-7) M) stimulates DNA synthesis at 18 hr of culture and inhibits [3H] thymidine uptake after 24 hr, while low concentrations of IL-2 toxin (10(-12)-10(-10) M) exhibits stimulatory effects only after 24 hr of culture. Tritium 172-174 interleukin 2 Homo sapiens 70-74 2583751-3 1989 The addition of sIL2R, purified by gel filtration chromatography, to cultures of PHA stimulated lymphoblasts resulted in a dose-dependent inhibition of [3H]TdR incorporation that could be overcome by the addition of exogenous IL2. Tritium 153-155 interleukin 2 Homo sapiens 17-20 2663733-5 1989 Similarly, GM-CSF augmented the proliferative response of lymphocytes to IL-2 in the presence of monocytes as assessed by 3H-TdR uptake. Tritium 122-124 interleukin 2 Homo sapiens 73-77 2645717-2 1989 The effect of exogenous interleukin 2 and/or B cell growth factor (BCGF) on these cells was measured by 3H-thymidine incorporation and immunoglobulin production. Tritium 104-106 interleukin 2 Homo sapiens 24-37 2785933-2 1989 PEMF-exposed cultures that presented increased [3H]thymidine incorporation showed lower amounts of IL-2 in their supernatants, but higher percentages of IL-2 receptor-positive cells and of T-activated lymphocytes. Tritium 48-50 interleukin 2 Homo sapiens 99-103 3264807-2 1988 Only IL-2 and IL-4 induced a marked stimulation of [3H] thymidine ([3H]TdR) uptake, cell proliferation and expression of activation markers [transferrin receptor (TrfR), IL-2R]. Tritium 68-70 interleukin 2 Homo sapiens 5-9 3264807-2 1988 Only IL-2 and IL-4 induced a marked stimulation of [3H] thymidine ([3H]TdR) uptake, cell proliferation and expression of activation markers [transferrin receptor (TrfR), IL-2R]. Tritium 52-54 interleukin 2 Homo sapiens 5-9 2976937-5 1988 IL-2 production in the blood lymphocyte culture supernatant of the DTG patients was assessed by testing on 3H-thymidine labeled ConA-T-blasts. Tritium 107-109 interleukin 2 Homo sapiens 0-4 3263216-3 1988 The cells responded rapidly to restimulation with conditioned medium containing IL-2 as assessed by increased incorporation of [3H]TdR into DNA. Tritium 128-130 interleukin 2 Homo sapiens 80-84 3132748-1 1988 The proliferative response of the peripheral mononuclear cells (MNC) from cancer patients and healthy individuals to IL-2 was studied by use of the quantitative microwell assay of the 3H-TdR incorporation into the cells in vitro. Tritium 184-186 interleukin 2 Homo sapiens 117-121 3127469-5 1988 Dose-response curves revealed that the assay correlates well with the proliferative response of the responder cells as measured by the [3H]thymidine (3H-TdR) uptake assay which is the commonly employed assay for quantitating IL-2. Tritium 136-138 interleukin 2 Homo sapiens 225-229 3127469-5 1988 Dose-response curves revealed that the assay correlates well with the proliferative response of the responder cells as measured by the [3H]thymidine (3H-TdR) uptake assay which is the commonly employed assay for quantitating IL-2. Tritium 150-152 interleukin 2 Homo sapiens 225-229 3127469-7 1988 In an attempt to further simplify the procedure, a novel approach involving an in situ assay was designed and was found to be applicable for quantitating IL-2 by both the 3H-TdR uptake assay and the IL-2R ELISA. Tritium 171-173 interleukin 2 Homo sapiens 154-158 2450923-3 1988 The specific inhibition of c-myc protein synthesis prevented the entry into S phase of resting T cells, induced to proliferate by PHA, or IL-2-dependent T cells induced by IL-2, as shown by [3H]thymidine incorporation. Tritium 191-193 interleukin 2 Homo sapiens 172-176 3140372-3 1988 While [3H]thymidine incorporation was characterized by a rather bell-shaped curve with increasing concentrations of PHA, IL-2 and TNF-alpha displayed a linear dose-dependent increase. Tritium 7-9 interleukin 2 Homo sapiens 121-125 3261574-2 1988 The proliferative response to mitogens and to IL-2 determined by measuring [3H]-thymidine incorporation 72 h after stimulation is generally decreased in AA patients. Tritium 76-78 interleukin 2 Homo sapiens 46-50 2460238-3 1988 Cellular responsiveness to graded levels of phytohemagglutinin (PHA), concanavalin A (Con A), and recombinant interleukin-2(r IL-2) was monitored by 3H-thymidine (3H-TdRO) uptake, production, and release of interleukin-2 (IL-2) and interleukin-2 receptor (IL-2R) and cytotoxic activities against K-562 and breast carcinoma (BCa) short-term cell lines. Tritium 149-151 interleukin 2 Homo sapiens 126-130 3501068-3 1987 Interleukin-2 was measured by the tritium-labelled thymidine CTLL assay on the supernatants of peripheral blood lymphocytes of gastric cancer patients after 24 hours stimulation with phytohemagglutinin. Tritium 34-41 interleukin 2 Homo sapiens 0-13 3036952-4 1987 Thus, the biological activities of the growth factors IL-2 or IL-1 could be detected and quantitated at the same level of sensitivity as with the conventional [3H]thymidine incorporation. Tritium 160-162 interleukin 2 Homo sapiens 54-58 3114593-2 1987 CEM T-cells that contained the virus expressed functional IL-2 receptors that transiently mediated five- to tenfold increases in [3H]thymidine incorporation following the addition of picomolar quantities of IL-2. Tritium 130-132 interleukin 2 Homo sapiens 58-62 3106493-2 1987 Stimulated but not resting monocytes displayed the Tac peptide of the interleukin 2 (IL 2) receptor within 24 hr as measured by immunofluorescence staining and [3H] Tac binding. Tritium 161-163 interleukin 2 Homo sapiens 70-83 3106493-2 1987 Stimulated but not resting monocytes displayed the Tac peptide of the interleukin 2 (IL 2) receptor within 24 hr as measured by immunofluorescence staining and [3H] Tac binding. Tritium 161-163 interleukin 2 Homo sapiens 85-89 3107631-6 1987 The demonstration of low concentrations of IL2 activity in the culture medium conditioned by the cells suggests that endogenous IL2 had been responsible for the spontaneous 3H-thymidine uptake by the CLL cells of patient 4. Tritium 173-175 interleukin 2 Homo sapiens 43-46 3107631-6 1987 The demonstration of low concentrations of IL2 activity in the culture medium conditioned by the cells suggests that endogenous IL2 had been responsible for the spontaneous 3H-thymidine uptake by the CLL cells of patient 4. Tritium 173-175 interleukin 2 Homo sapiens 128-131 3035707-4 1987 On the contrary, IL-2-induced [3H]thymidine incorporation was inhibited by these drugs. Tritium 31-33 interleukin 2 Homo sapiens 17-21 3031160-4 1987 A proliferative response, as measured by [3H]thymidine uptake of B-A3 cells was significantly induced by either commercial IL 2-free human BCGF preparations, or phytohemagglutinin-stimulated mixed lymphocyte culture supernatant at all FCS concentrations used in the assay. Tritium 42-44 interleukin 2 Homo sapiens 123-127 3113151-2 1987 A recombinant IL-2 (rIL-2) and an IL-2 containing conditioned medium (LyIL-2) markedly enhanced 3H-thymidine incorporations at low PHA concentrations, whereas at optimal mitogen concentrations, this effect was marginal. Tritium 96-98 interleukin 2 Homo sapiens 14-18 3514800-11 1986 All IL-2s stimulated the tritiated thymidine [3H]TdR incorporation of human PBMC in the absence of other stimuli, in addition to augmenting the response to mitogen or alloantigens. Tritium 46-48 interleukin 2 Homo sapiens 4-8 3005412-8 1986 Additional analysis of IL 2 receptor induced in culture with IL 2 was performed by [125I]anti-TAC binding and by [3H]IL 2 binding. Tritium 114-116 interleukin 2 Homo sapiens 23-27 3005412-8 1986 Additional analysis of IL 2 receptor induced in culture with IL 2 was performed by [125I]anti-TAC binding and by [3H]IL 2 binding. Tritium 114-116 interleukin 2 Homo sapiens 61-65 3005412-8 1986 Additional analysis of IL 2 receptor induced in culture with IL 2 was performed by [125I]anti-TAC binding and by [3H]IL 2 binding. Tritium 114-116 interleukin 2 Homo sapiens 61-65 3005412-9 1986 Scatchard analysis of [3H]IL 2 binding, in the range of concentrations leading to the detection of high-affinity binding sites, showed an affinity constant similar to that of conventional phytohemagglutinin blasts. Tritium 23-25 interleukin 2 Homo sapiens 26-30 3493021-2 1987 Extracts of the herb were found to inhibit mitogen-induced tritiated thymidine ([3H]-TdR) uptake by human peripheral blood mononuclear cells (PBMC), interleukin 2 (IL-2)-induced [3H]-TdR uptake by lymphoblasts and PGE2 release by interleukin 1 (IL-1)-stimulated synovial cells. Tritium 179-181 interleukin 2 Homo sapiens 164-168 2433050-5 1986 3H-TdR uptake assays and FC showed that after three cycles of incubation in the presence of interleukin-2 (IL-2) or antigen, the T cell lines had increased specificity and responsiveness to the priming antigen and were enriched for the L3T4 (helper/inducer) phenotype. Tritium 0-2 interleukin 2 Homo sapiens 92-105 2433050-5 1986 3H-TdR uptake assays and FC showed that after three cycles of incubation in the presence of interleukin-2 (IL-2) or antigen, the T cell lines had increased specificity and responsiveness to the priming antigen and were enriched for the L3T4 (helper/inducer) phenotype. Tritium 0-2 interleukin 2 Homo sapiens 107-111 3489670-4 1986 [3H]TdR incorporation by IL-2-dependent human T cells after growth in IL-2 or SF alone for 14 days was slight, but significant. Tritium 1-3 interleukin 2 Homo sapiens 25-29 3489670-4 1986 [3H]TdR incorporation by IL-2-dependent human T cells after growth in IL-2 or SF alone for 14 days was slight, but significant. Tritium 1-3 interleukin 2 Homo sapiens 70-74 3489670-5 1986 By contrast, growth in a combination of SF and IL-2 for 14 days stimulated [3H]TdR incorporation 10-20-fold higher, generally equal to the high incorporation measured when cells were grown in the presence of the conditioned medium from which SF was obtained. Tritium 76-78 interleukin 2 Homo sapiens 47-51 3082978-8 1986 In contrast, under similar conditions, IL 2 induced significant increases in [3H]thymidine incorporation and cell proliferation. Tritium 78-80 interleukin 2 Homo sapiens 39-43 3005464-6 1986 Light-microscopic autoradiography with 3H-labeled IL-2 confirmed the internalization of IL-2, and suggested that some IL-2 might be carried to the nucleus. Tritium 39-41 interleukin 2 Homo sapiens 50-54 3005464-6 1986 Light-microscopic autoradiography with 3H-labeled IL-2 confirmed the internalization of IL-2, and suggested that some IL-2 might be carried to the nucleus. Tritium 39-41 interleukin 2 Homo sapiens 88-92 3005464-6 1986 Light-microscopic autoradiography with 3H-labeled IL-2 confirmed the internalization of IL-2, and suggested that some IL-2 might be carried to the nucleus. Tritium 39-41 interleukin 2 Homo sapiens 88-92 3097428-1 1986 Interleukin 2 (IL-2) receptors which are expressed in T-lymphocytes after antigenic or mitogenic stimulation have been measured with either 3H- or 125J-labelled IL-2 or with receptor antibodies (e.g. anti-Tac). Tritium 140-142 interleukin 2 Homo sapiens 0-13 3097428-1 1986 Interleukin 2 (IL-2) receptors which are expressed in T-lymphocytes after antigenic or mitogenic stimulation have been measured with either 3H- or 125J-labelled IL-2 or with receptor antibodies (e.g. anti-Tac). Tritium 140-142 interleukin 2 Homo sapiens 15-19 3097428-4 1986 Total bound IL-2 is determined by a conventional 3H-thymidine incorporation assay. Tritium 49-51 interleukin 2 Homo sapiens 12-16 3876783-3 1985 Interleukin-2 production was measured by the tritium-labeled thymidine CT6 assay on the supernatants of peripheral blood lymphocytes of head and neck cancer patients and controls after 24 hour stimulation with phytohemagglutinin. Tritium 45-52 interleukin 2 Homo sapiens 0-13 3100885-4 1986 Here, in five of 11 B-cell leukemia/lymphoma cases, we identified cells which not only express the IL-2 receptor, but also respond to IL-2 stimulation, as shown by a marked increase of 3H-thymidine incorporation and by differentiation of lymphoma cells. Tritium 185-187 interleukin 2 Homo sapiens 99-103 6980181-2 1982 Il-2 dependent growth, as measured by 3H-TDR incorporation or viable cell counts was enhanced by PMA (5-100 ng/ml) and inhibited by Con A. Tritium 38-40 interleukin 2 Homo sapiens 0-4 3935432-3 1985 Two major polypeptide species (interleukin-2 N1 and N2, 16.5 kDa) were shown to be glycosylated on the basis of [3H]galactose/[3H]glucosamine incorporation and determination of amino sugars after acid hydrolysis. Tritium 113-115 interleukin 2 Homo sapiens 31-44 3935432-3 1985 Two major polypeptide species (interleukin-2 N1 and N2, 16.5 kDa) were shown to be glycosylated on the basis of [3H]galactose/[3H]glucosamine incorporation and determination of amino sugars after acid hydrolysis. Tritium 127-129 interleukin 2 Homo sapiens 31-44 2931095-4 1985 However, [3H]-thymidine incorporation by azathioprine (36 microM) + interleukin-2 treated MLR was 4.3 times greater than that exposed to azathioprine alone. Tritium 10-12 interleukin 2 Homo sapiens 68-81 3917278-5 1985 Consistent with these observations, culturing PBMC with PHA plus WGA markedly decreased the number of high-affinity IL 2 receptor per cell, as determined by binding of purified [3H]IL 2, relative to cultures containing PHA alone. Tritium 178-180 interleukin 2 Homo sapiens 116-120 3917278-5 1985 Consistent with these observations, culturing PBMC with PHA plus WGA markedly decreased the number of high-affinity IL 2 receptor per cell, as determined by binding of purified [3H]IL 2, relative to cultures containing PHA alone. Tritium 178-180 interleukin 2 Homo sapiens 181-185 6234069-7 1984 These results show that Con A-treated MN cells suppressed or prevented [3H]thymidine incorporation by actively inhibiting IL-2 production. Tritium 72-74 interleukin 2 Homo sapiens 122-126 6417659-2 1983 We now show that: (i) TCGF blocks 85% of 3H-labeled anti-Tac binding to phytohemagglutinin-activated lymphoblasts and (ii) both anti-Tac and anti-TCGF immunoprecipitate a protein band that appears to represent TCGF crosslinked to its receptor on HUT-102B2 cells. Tritium 41-43 interleukin 2 Homo sapiens 22-26 6984451-2 1982 IL2 when tested in a [3H]thymidine incorporation assay by human IL2-dependent T cells, appeared to have a MW of 25,000 as determined by Ultrogel ACA 54 gel filtration. Tritium 22-24 interleukin 2 Homo sapiens 0-3 7106972-0 1982 Lymphokine-induced uptake of [14C]glucosamine, [14C]glucose, and [3H]leucine by macrophages. Tritium 66-68 interleukin 2 Homo sapiens 0-10 3925347-5 1985 We show here, using biosynthetically labelled IL-2(3H-IL-2) and anti-IL-2 receptor antibody (3H-PC61) that activated B and T cells express both high-affinity (apparent dissociation constant, Kd approximately 20 pM) and low-affinity (Kd approximately 1,000 pM) IL-2 receptors. Tritium 51-53 interleukin 2 Homo sapiens 46-50 3925347-5 1985 We show here, using biosynthetically labelled IL-2(3H-IL-2) and anti-IL-2 receptor antibody (3H-PC61) that activated B and T cells express both high-affinity (apparent dissociation constant, Kd approximately 20 pM) and low-affinity (Kd approximately 1,000 pM) IL-2 receptors. Tritium 51-53 interleukin 2 Homo sapiens 54-58 3925347-5 1985 We show here, using biosynthetically labelled IL-2(3H-IL-2) and anti-IL-2 receptor antibody (3H-PC61) that activated B and T cells express both high-affinity (apparent dissociation constant, Kd approximately 20 pM) and low-affinity (Kd approximately 1,000 pM) IL-2 receptors. Tritium 51-53 interleukin 2 Homo sapiens 54-58 3917474-2 1985 B73.1+ NK cells, cultured for 6 days in the presence of conditioned medium containing IL 2 and irradiated B lymphoblastoid cells, show significant [3H]TdR incorporation beginning on day 4. Tritium 148-150 interleukin 2 Homo sapiens 86-90 6610479-6 1984 However, when IL-2 was removed from the cultures, the [3H]thymidine incorporation per 10(4) cells and correspondingly the number of cells in the S/G2/M and G1 phases were reduced drastically and during the following 72-hr period, the number of G0 cells increased markedly. Tritium 55-57 interleukin 2 Homo sapiens 14-18 6412230-6 1983 However, 7D4 specifically immunoprecipitated 3H-labeled human IL-2 from detergent extracts of HT2 cells or Con A-induced T-cell blasts that had been pulsed with [3H]IL-2; in contrast, 7D4 did not react with free [3H]IL-2. Tritium 45-47 interleukin 2 Homo sapiens 62-66 26407095-10 2015 PBMC IL-2 and NK cell cytotoxic activity were greater (P < 0.05) 3h post exercise in the krill oil compared to the control group. Tritium 68-70 interleukin 2 Homo sapiens 5-9 23189555-8 2012 The 3H-labeled peptide corresponding to the 59-72 sequence ofthe human IL-2 was shown to bind to the CTLL cels. Tritium 4-6 interleukin 2 Homo sapiens 71-75