PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16122793-4 2006 PAF was measured by PAF [3H] scintillation proximity assay (SPA) system. Tritium 25-27 PCNA clamp associated factor Homo sapiens 0-3 16426477-3 2007 PMS777 (1-100 microM) dose-dependently inhibited PAF-induced rabbit platelet aggregation by competing with [3H]PAF for its receptor on platelets, and protected a human neuroblastoma cell line SH-SY5Y against PAF-induced neurotoxicity. Tritium 108-110 PCNA clamp associated factor Homo sapiens 49-52 16426477-3 2007 PMS777 (1-100 microM) dose-dependently inhibited PAF-induced rabbit platelet aggregation by competing with [3H]PAF for its receptor on platelets, and protected a human neuroblastoma cell line SH-SY5Y against PAF-induced neurotoxicity. Tritium 108-110 PCNA clamp associated factor Homo sapiens 111-114 16426477-3 2007 PMS777 (1-100 microM) dose-dependently inhibited PAF-induced rabbit platelet aggregation by competing with [3H]PAF for its receptor on platelets, and protected a human neuroblastoma cell line SH-SY5Y against PAF-induced neurotoxicity. Tritium 108-110 PCNA clamp associated factor Homo sapiens 111-114 16122793-4 2006 PAF was measured by PAF [3H] scintillation proximity assay (SPA) system. Tritium 25-27 PCNA clamp associated factor Homo sapiens 20-23 11804958-7 2002 Production of PAF and lyso-PAF was measured with a biological assay using [3H]serotonin release from rabbit platelets. Tritium 75-77 PCNA clamp associated factor Homo sapiens 14-17 15890711-7 2005 EFS-induced [3H]ACh release from muscle strips significantly (42%) decreased in esophagitis and after 2 h incubation in PGE2 and in PAF C-16. Tritium 13-15 PCNA clamp associated factor Homo sapiens 132-135 11804958-7 2002 Production of PAF and lyso-PAF was measured with a biological assay using [3H]serotonin release from rabbit platelets. Tritium 75-77 PCNA clamp associated factor Homo sapiens 27-30 10555184-6 1999 (3H)PAF binding to platelets of stroke patients was lower than in controls (149.58 +/- 46.11 and 212.1 +/- 10.3 receptors cell-1, respectively, p < 0.001) and was significantly correlated with infarct volume (r = -0.606, p = 0.014) and with neurological score (r = 0.527, p = 0.032). Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 10737702-2 2000 All myeloma cell lines express a PAF acetylhydrolase activity and metabolize [3H]PAF and [3H]lyso PAF in 1-alkyl-2-acyl analogue of phosphatidylcholine. Tritium 78-80 PCNA clamp associated factor Homo sapiens 81-84 9219895-3 1997 PAF accumulation was accompanied by the activation of the CoA-independent transacylase and acetyl-CoA: lyso-PAF acetyltransferase, whereas the release of free [3H]arachidonic acid in prelabeled cells reflecting the activation of phospholipase A2, occurred primarily within the initial 1-5 min of treatment with LPS. Tritium 160-163 PCNA clamp associated factor Homo sapiens 0-3 9434130-8 1997 PAF (1-100 nM) stimulated [3H]thymidine incorporation in K562 cells grown in low serum concentration, a non-metabolizable PAF agonist being more potent than PAF to stimulate thymidine incorporation. Tritium 27-29 PCNA clamp associated factor Homo sapiens 0-3 9246604-3 1997 PAF (1 microM) stimulates and PAF antagonists (0.1-1 microM) inhibit [3H]thymidine incorporation in cells grown in 5% serum. Tritium 70-72 PCNA clamp associated factor Homo sapiens 0-3 9246604-3 1997 PAF (1 microM) stimulates and PAF antagonists (0.1-1 microM) inhibit [3H]thymidine incorporation in cells grown in 5% serum. Tritium 70-72 PCNA clamp associated factor Homo sapiens 30-33 9157961-5 1997 [3H]PAF bound to adherent human macrophages with a K(d) of 2.1 nmol/L and a B(max) of 19 fmol/10(6) cells; approximately 5300 binding sites per cell were detected. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 9042336-7 1997 We also found that genistein and methyl 2,5-dihydroxycinnamate decreased both basal and PAF-stimulated [3H]thymidine uptake in these cells. Tritium 104-106 PCNA clamp associated factor Homo sapiens 88-91 9101422-5 1997 Experiments with [3H]lyso PAF indicate that human mononuclear bone marrow cells and marrow stromal cells actively acylate lyso PAF into a 1-alkyl analogue of phosphatidylcholine. Tritium 18-21 PCNA clamp associated factor Homo sapiens 127-130 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 52-54 PCNA clamp associated factor Homo sapiens 55-58 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 52-54 PCNA clamp associated factor Homo sapiens 146-149 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 143-145 PCNA clamp associated factor Homo sapiens 55-58 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 143-145 PCNA clamp associated factor Homo sapiens 146-149 9042336-9 1997 PAF-stimulated [3H]thymidine uptake was also prevented by PKC down regulation after long term exposure to PMA and PKC inhibition with the two inhibitors sangivamycin and bis-indolylmaleimide. Tritium 16-18 PCNA clamp associated factor Homo sapiens 0-3 8645989-4 1996 Excess unlabeled PAF (500 nM) or the specific PAF receptor antagonist WEB 2086 (1 microM) inhibited the [3H]PAF binding. Tritium 105-107 PCNA clamp associated factor Homo sapiens 17-20 8858930-4 1996 PAF increased the production of 3H-labeled inositol phosphates (IPs) with EC50 values of 1.2-1.5 nM. Tritium 32-34 PCNA clamp associated factor Homo sapiens 0-3 8858930-5 1996 The effect of PAF on 3H-IPs formation was completely blocked by the PAF antagonist BN 50739 at a concentration of 300 nM. Tritium 21-23 PCNA clamp associated factor Homo sapiens 14-17 8858930-5 1996 The effect of PAF on 3H-IPs formation was completely blocked by the PAF antagonist BN 50739 at a concentration of 300 nM. Tritium 21-23 PCNA clamp associated factor Homo sapiens 68-71 8858930-10 1996 Moreover, specific [3H]PAF binding sites coprecipitated with G alpha q and G alpha i1/2 proteins. Tritium 20-22 PCNA clamp associated factor Homo sapiens 23-26 8832056-15 1996 The incorporation of [3H]-acetate into [3H]-PAF induced by serum-treated zymosan in human PMNs was also inhibited concentration-dependently by cloricromene, with an IC50 of 105 microM. Tritium 22-24 PCNA clamp associated factor Homo sapiens 44-47 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 14-17 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 8645989-4 1996 Excess unlabeled PAF (500 nM) or the specific PAF receptor antagonist WEB 2086 (1 microM) inhibited the [3H]PAF binding. Tritium 105-107 PCNA clamp associated factor Homo sapiens 46-49 8645989-4 1996 Excess unlabeled PAF (500 nM) or the specific PAF receptor antagonist WEB 2086 (1 microM) inhibited the [3H]PAF binding. Tritium 105-107 PCNA clamp associated factor Homo sapiens 46-49 8726354-3 1996 Both alpha 2M-methylamine and a cloned and expressed 20-kDa receptor binding fragment (RBF) from rat alpha 1M+, stimulated macrophage synthesis of PAF from [3H]acetate, [3H]methylcholine, and 1-O-[3H]alkyl lyso-PAF by two- to threefold. Tritium 157-159 PCNA clamp associated factor Homo sapiens 147-150 8906117-2 1996 Specific binding of [3H]WEB 2086, a specific ligand for PAF receptor, to peripheral blood eosinophils obtained from healthy individuals was significantly increased when the cells were cultured with IL-3, IL-5, or GM-CSF for 12 h, compared with untreated cells. Tritium 21-23 PCNA clamp associated factor Homo sapiens 56-59 8624843-14 1996 Blood cardiolipin competes [3H]PAF binding in rabbit platelets. Tritium 28-30 PCNA clamp associated factor Homo sapiens 31-34 7786298-5 1995 A receptor binding study using radiolabelled [3H]WEB 2086 showed that Et-16-OCH3 exerts its actions through interaction with the PAF receptor. Tritium 46-48 PCNA clamp associated factor Homo sapiens 129-132 7594622-2 1995 The CHO cells expressing the PAF receptor, termed CHO.1F8, showed a significant intracellular Ca2+ response to PAF, and the same binding properties to [3H]WEB 2086, a PAF antagonist, as reported (Kd, 13.6 +/- 1.9 nM; Bmax, 2.5 +/- 0.4 pmol/mg protein (n = 6)). Tritium 152-154 PCNA clamp associated factor Homo sapiens 29-32 7689100-1 1993 A431 cells, a human epidermoid carcinoma, possess specific [3H]platelet-activating factor (PAF) and [3H]WEB 2086 binding sites indicating the presence of PAF receptors. Tritium 60-62 PCNA clamp associated factor Homo sapiens 154-157 7857985-7 1995 Experiments with [3H]PAF indicate that human bone marrow cells actively metabolize this potent molecule by the deacetylation-transacylation pathway. Tritium 18-20 PCNA clamp associated factor Homo sapiens 21-24 8295490-9 1994 The recovery rate of PAF throughout this procedure was constant and satisfactory (37.4 +/- 9.7%), which was confirmed using [3H]-PAF. Tritium 125-127 PCNA clamp associated factor Homo sapiens 21-24 8295490-9 1994 The recovery rate of PAF throughout this procedure was constant and satisfactory (37.4 +/- 9.7%), which was confirmed using [3H]-PAF. Tritium 125-127 PCNA clamp associated factor Homo sapiens 129-132 8410799-8 1993 When 25 g albumin l-1 was added, 22% of [3H]PAF was hydrolysed h-1 compared with 72% in media without albumin. Tritium 41-43 PCNA clamp associated factor Homo sapiens 44-47 7848283-3 1995 We report here that differentiation of a human eosinophilic leukaemia cell line, EoL-1, by sodium n-butyrate is associated with induction of PAF receptor gene expression, as indicated by: PAF receptor mRNA accumulation; increases in the binding of [3H]WEB 2086, a PAF antagonist; analysis of cell-surface expression of PAF receptor protein using a monoclonal anti-(PAF receptor) antibody; and augmentation of PAF-induced increase in the intracellular concentration of calcium. Tritium 249-251 PCNA clamp associated factor Homo sapiens 141-144 8004455-0 1994 Changes in [3H]PAF binding and PAF concentrations in gerbil brain after bilateral common carotid artery occlusion: a quantitative autoradiographic study. Tritium 12-14 PCNA clamp associated factor Homo sapiens 15-18 8004455-2 1994 In sagittal sections of gerbil brain, labeling with [3H]PAF was mainly located in the cortex, hippocampus and cerebellum. Tritium 53-55 PCNA clamp associated factor Homo sapiens 56-59 8004455-8 1994 These results indicate that PAF may be involved in the early stages of brain ischemia in the gerbil and suggest that endogenous PAF produced during ischemia may contribute to the down-regulation of [3H]PAF binding sites in the brain. Tritium 199-201 PCNA clamp associated factor Homo sapiens 28-31 8004455-8 1994 These results indicate that PAF may be involved in the early stages of brain ischemia in the gerbil and suggest that endogenous PAF produced during ischemia may contribute to the down-regulation of [3H]PAF binding sites in the brain. Tritium 199-201 PCNA clamp associated factor Homo sapiens 128-131 8004455-8 1994 These results indicate that PAF may be involved in the early stages of brain ischemia in the gerbil and suggest that endogenous PAF produced during ischemia may contribute to the down-regulation of [3H]PAF binding sites in the brain. Tritium 199-201 PCNA clamp associated factor Homo sapiens 128-131 8118633-9 1994 We found that the time required for deacetylation of 50% of [3H]PAF (t1/2) to lyso-PAF was 50 min in baseline secretions and 10 and 22 min in NLF obtained 10 min and 24 h after antigen challenge, respectively. Tritium 61-63 PCNA clamp associated factor Homo sapiens 64-67 8118633-9 1994 We found that the time required for deacetylation of 50% of [3H]PAF (t1/2) to lyso-PAF was 50 min in baseline secretions and 10 and 22 min in NLF obtained 10 min and 24 h after antigen challenge, respectively. Tritium 61-63 PCNA clamp associated factor Homo sapiens 83-86 8280772-2 1994 They were, respectively, 5-, 300-, 500-, and 4000-fold weaker than PAF in each assay; inhibited PMN-binding of [3H]PAF at concentrations paralleling their biological potencies; and showed sensitivity to the inhibitory effects of PAF antagonists. Tritium 111-115 PCNA clamp associated factor Homo sapiens 67-70 8227034-9 1993 Whereas PAF production seems extremely sensitive to changes in [Ca2+]i, which seems to exert its modulatory effect at the lyso-PAF:acetyl-CoA acetyltransferase step, [3H]arachidonate release seems tightly modulated by protein kinase C-dependent mechanisms and is coincidental with activation of phospholipase D. Tritium 167-170 PCNA clamp associated factor Homo sapiens 8-11 8400115-3 1993 The appearance of PAF was assessed by measuring the number of freely accessible PAF-receptors on platelets in vitro, assuming that the contact between platelets and PAF in vivo would reduce the receptor binding of [3H]PAF in vitro. Tritium 215-217 PCNA clamp associated factor Homo sapiens 18-21 8503848-5 1993 Most of the newly synthesized [3H]PAF formed in response to acrosome reaction was found in the supernatant, suggesting a release of the phospholipid from spermatozoa. Tritium 31-33 PCNA clamp associated factor Homo sapiens 34-37 7681399-5 1993 Furthermore, L-659,989 and PAF inhibited specific 3H-labeled PAF binding in TNF-treated, but not in untreated cells. Tritium 50-52 PCNA clamp associated factor Homo sapiens 27-30 7681399-5 1993 Furthermore, L-659,989 and PAF inhibited specific 3H-labeled PAF binding in TNF-treated, but not in untreated cells. Tritium 50-52 PCNA clamp associated factor Homo sapiens 61-64 1419027-5 1992 PAF was quantified by thin-layer chromatographic separation of lipid extracts from cells radiolabeled with [3H]lysoPAF and by radioimmunoassay. Tritium 108-110 PCNA clamp associated factor Homo sapiens 0-3 1332696-5 1992 However, tracking studies showed that [3H]PAF accumulated on the cell surface for approximately 2 min before being internalized. Tritium 39-41 PCNA clamp associated factor Homo sapiens 42-45 1419027-8 1992 Primary human bronchial epithelial cells also demonstrated significant increases in [3H]PAF release (average increase of 289% after exposure to 1.0 ppm O3 for 60 min) compared with paired air controls. Tritium 85-87 PCNA clamp associated factor Homo sapiens 88-91 1322030-11 1992 In displacement experiments PAF (0.01 to 10000 nmol/L) and WEB 2086 (0.01 to 10000 nmol/L), but not lyso-PAF, completely displaced 3H-WEB 2086 from its binding sites on human and lung membranes. Tritium 131-133 PCNA clamp associated factor Homo sapiens 28-31 1527040-1 1992 Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, PAF) labeled with 3H in the alkyl side chain was taken up rapidly by amnion-derived WISH cells in culture. Tritium 94-96 PCNA clamp associated factor Homo sapiens 76-79 1334723-2 1992 This property has been applied to the measurement of PAF in circulating blood, the concept being that in vivo contact with endogenous PAF diminishes subsequent in vitro binding of 3H-labeled PAF. Tritium 180-182 PCNA clamp associated factor Homo sapiens 53-56 1334723-2 1992 This property has been applied to the measurement of PAF in circulating blood, the concept being that in vivo contact with endogenous PAF diminishes subsequent in vitro binding of 3H-labeled PAF. Tritium 180-182 PCNA clamp associated factor Homo sapiens 134-137 1334723-2 1992 This property has been applied to the measurement of PAF in circulating blood, the concept being that in vivo contact with endogenous PAF diminishes subsequent in vitro binding of 3H-labeled PAF. Tritium 180-182 PCNA clamp associated factor Homo sapiens 134-137 1334723-3 1992 Here we show that, when platelets are stimulated with thrombin, the subsequent binding of [3H]PAF is greatly reduced, probably due to proteolytic damage of the PAF receptors. Tritium 91-93 PCNA clamp associated factor Homo sapiens 94-97 1334723-3 1992 Here we show that, when platelets are stimulated with thrombin, the subsequent binding of [3H]PAF is greatly reduced, probably due to proteolytic damage of the PAF receptors. Tritium 91-93 PCNA clamp associated factor Homo sapiens 160-163 1334723-4 1992 In addition, [3H]PAF binding is decreased, when the cell"s energy status is low, which is seen during thrombin-induced secretion. Tritium 14-16 PCNA clamp associated factor Homo sapiens 17-20 1450138-1 1992 OBJECTIVE: To determine the metabolism of 3H-Platelet-activating factor (3H-PAF) during transfer through human fetal membranes. Tritium 42-44 PCNA clamp associated factor Homo sapiens 76-79 1400315-7 1992 With intact differentiated HL-60 cells, [3H]acetate from [3H]PAF can be incorporated into alk-1-enylacetyl-GPE in the presence of ionophore A23187, but not in its absence. Tritium 41-43 PCNA clamp associated factor Homo sapiens 61-64 1321849-7 1992 The activity of acetyl CoA:2-lyso-PAF acetyltransferase was transiently increased in porin-stimulated PMN and the [3H]acetyl group was incorporated in the synthetized PAF after cell preincubation with [3H]acetyl CoA. Tritium 115-117 PCNA clamp associated factor Homo sapiens 34-37 1321849-7 1992 The activity of acetyl CoA:2-lyso-PAF acetyltransferase was transiently increased in porin-stimulated PMN and the [3H]acetyl group was incorporated in the synthetized PAF after cell preincubation with [3H]acetyl CoA. Tritium 202-204 PCNA clamp associated factor Homo sapiens 34-37 1514698-3 1992 Examination of the binding profile of tritium-labeled PAF to these platelets in the presence or absence of ADP revealed significant difference in the Kd values but not in the number of specific binding sites. Tritium 38-45 PCNA clamp associated factor Homo sapiens 54-57 1324692-6 1992 The order of potency for inhibition of [3H]thymidine uptake by WEHI-3B and HL-60 cells was (R)-ET-16-OCH3-GPC = (S)-ET-16-OCH3-GPC greater than (S)-PAF greater than (R)-PAF; the EC50 values were 2, 2, 15, and greater than 40 microM, respectively. Tritium 40-42 PCNA clamp associated factor Homo sapiens 148-151 1637948-2 1992 The mechanism of phosphatidylcholine (PC) degradation stimulated by PAF was investigated in endometrial explants prelabeled with [methyl-3H]choline or preincubated with [3H]butan-1-ol. Tritium 137-139 PCNA clamp associated factor Homo sapiens 68-71 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 144-146 PCNA clamp associated factor Homo sapiens 45-48 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 157-159 PCNA clamp associated factor Homo sapiens 45-48 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 157-159 PCNA clamp associated factor Homo sapiens 45-48 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 157-159 PCNA clamp associated factor Homo sapiens 45-48 1637948-4 1992 Within 30 sec there was a rapid rise in PAF-induced [3H]Cho generation and by 2 min this had increased to 59.9% +/- 10.6% (p less than 0.02), with no effect upon [3H]ChoP and [3H]GPC during this period. Tritium 53-55 PCNA clamp associated factor Homo sapiens 40-43 1525356-3 1992 The dose range of unlabeled PAF required to produce inhibition of [3H]PAF binding was roughly the same as that for induction of chemotaxis, and was much higher than that in platelets as reported in the literature. Tritium 67-69 PCNA clamp associated factor Homo sapiens 28-31 1525356-3 1992 The dose range of unlabeled PAF required to produce inhibition of [3H]PAF binding was roughly the same as that for induction of chemotaxis, and was much higher than that in platelets as reported in the literature. Tritium 67-69 PCNA clamp associated factor Homo sapiens 70-73 1312103-4 1992 Furthermore, L-659,989 (10 microM), as well as PAF (1 microM), inhibited specific [3H]PAF binding in LPS-treated but not in untreated cells. Tritium 83-85 PCNA clamp associated factor Homo sapiens 47-50 1312103-4 1992 Furthermore, L-659,989 (10 microM), as well as PAF (1 microM), inhibited specific [3H]PAF binding in LPS-treated but not in untreated cells. Tritium 83-85 PCNA clamp associated factor Homo sapiens 86-89 1541904-3 1992 After incubation for 1 h at 37 degrees C, formation of [3H]acyl-PAF from either [3H]lyso-PAF or [3H]PAF increased significantly in PMNs from infected guinea pigs compared to control PMNs. Tritium 56-59 PCNA clamp associated factor Homo sapiens 64-67 1541904-3 1992 After incubation for 1 h at 37 degrees C, formation of [3H]acyl-PAF from either [3H]lyso-PAF or [3H]PAF increased significantly in PMNs from infected guinea pigs compared to control PMNs. Tritium 56-59 PCNA clamp associated factor Homo sapiens 89-92 1541904-3 1992 After incubation for 1 h at 37 degrees C, formation of [3H]acyl-PAF from either [3H]lyso-PAF or [3H]PAF increased significantly in PMNs from infected guinea pigs compared to control PMNs. Tritium 56-59 PCNA clamp associated factor Homo sapiens 89-92 1643272-2 1992 [3H]PAF added to the cell lysate was similarly distributed after chromatography. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 1324692-6 1992 The order of potency for inhibition of [3H]thymidine uptake by WEHI-3B and HL-60 cells was (R)-ET-16-OCH3-GPC = (S)-ET-16-OCH3-GPC greater than (S)-PAF greater than (R)-PAF; the EC50 values were 2, 2, 15, and greater than 40 microM, respectively. Tritium 40-42 PCNA clamp associated factor Homo sapiens 169-172 1662484-5 1991 PAF enhanced [3H]glycerol incorporation into PtdOH and DG by 2- and 3-fold respectively during 1-10 min incubations. Tritium 14-16 PCNA clamp associated factor Homo sapiens 0-3 1738121-2 1992 TCV-309 specifically inhibited PAF-induced aggregation of rabbit and human platelets, and [3H]PAF binding to rabbit platelet microsomes with IC50 values of 33, 58 and 27 nM, respectively. Tritium 91-93 PCNA clamp associated factor Homo sapiens 94-97 1668105-7 1991 Scatchard analysis of [3H]PAF binding to monocyte membranes revealed 651 +/- 495 binding sites/monocyte with a Kd of 4.7 +/- 4.2 x 10(-10) M. PAF is a structurally unique activator of monocytes whose interactions with TNF alpha and other cytokines may be critical to host defense against tumors. Tritium 23-25 PCNA clamp associated factor Homo sapiens 26-29 1813180-4 1991 Based on [3H]PAF-binding studies, an obstruction of PAF-binding or the signal transduction by the branched alkyl chain in C-1 position of the glycerol backbone is suggested. Tritium 10-12 PCNA clamp associated factor Homo sapiens 13-16 1813180-4 1991 Based on [3H]PAF-binding studies, an obstruction of PAF-binding or the signal transduction by the branched alkyl chain in C-1 position of the glycerol backbone is suggested. Tritium 10-12 PCNA clamp associated factor Homo sapiens 52-55 1659411-2 1991 [3H]PAF binding to these cells was time-dependent, reaching equilibrium at 60 minutes, and saturable. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 1862226-4 1991 Synthesis of [3H] PAF was measured by counts comigrating on TLC with unlabeled PAF. Tritium 14-16 PCNA clamp associated factor Homo sapiens 18-21 1935836-7 1991 An inhibition of [3H]choline uptake and incorporation into phospholipids of fetal human lung explants and fetal lung type II pneumonocytes was induced by PAF. Tritium 18-20 PCNA clamp associated factor Homo sapiens 154-157 2051992-1 1991 Specific binding of tritium-labeled platelet-activating factor (PAF) and a nonmetabolizable bioactive analog of PAF, 1-O-alkyl-2-N-methylcarbamyl-sn-glyceryl-3-phosphorylcholine, to human platelet membranes was found to be potentiated by wheat germ agglutinin (WGA) and erythroagglutinin. Tritium 20-27 PCNA clamp associated factor Homo sapiens 36-62 2051992-1 1991 Specific binding of tritium-labeled platelet-activating factor (PAF) and a nonmetabolizable bioactive analog of PAF, 1-O-alkyl-2-N-methylcarbamyl-sn-glyceryl-3-phosphorylcholine, to human platelet membranes was found to be potentiated by wheat germ agglutinin (WGA) and erythroagglutinin. Tritium 20-27 PCNA clamp associated factor Homo sapiens 64-67 1795022-2 1991 HL-60 cells differentiated to macrophages (HL-60/M phi) with a phorbol ester convert added [3H]PAF to AAG; 22% of the incorporated radioactivity is converted to AAG within 15s. Tritium 92-94 PCNA clamp associated factor Homo sapiens 95-98 1650834-4 1991 In competition studies, RP 59227 produced dextral and concentration-dependent shifts of the sigmoidal inhibition curve of [3H]PAF binding by PAF(C16). Tritium 123-125 PCNA clamp associated factor Homo sapiens 126-129 1650834-4 1991 In competition studies, RP 59227 produced dextral and concentration-dependent shifts of the sigmoidal inhibition curve of [3H]PAF binding by PAF(C16). Tritium 123-125 PCNA clamp associated factor Homo sapiens 141-148 1862226-4 1991 Synthesis of [3H] PAF was measured by counts comigrating on TLC with unlabeled PAF. Tritium 14-16 PCNA clamp associated factor Homo sapiens 79-82 2322233-2 1990 The bulk of the bound [3H]-PAF eluted with a higher molecular weight species of PCBP, possibly an aggregated form of PCBP. Tritium 23-25 PCNA clamp associated factor Homo sapiens 27-30 2005325-6 1991 There was a good correlation (r = 0.95) between PAF quantified by [3H]acetic acid incorporation and by rabbit platelet aggregation bioassay, indicating that the radioactive material is PAF. Tritium 67-69 PCNA clamp associated factor Homo sapiens 48-51 2005325-6 1991 There was a good correlation (r = 0.95) between PAF quantified by [3H]acetic acid incorporation and by rabbit platelet aggregation bioassay, indicating that the radioactive material is PAF. Tritium 67-69 PCNA clamp associated factor Homo sapiens 185-188 2246516-6 1990 When exogenous [3H]PAF was added to the two stimulated eosinophil populations subsequent analysis of the [3H]PAF metabolites by DIOL-HPLC revealed that low density eosinophils incorporated PAF into the phosphatidylcholine (PC) pool more rapidly than did normal density eosinophils or neutrophils. Tritium 106-108 PCNA clamp associated factor Homo sapiens 19-22 2096302-6 1990 [3H]PAF binding studies provided evidence that KO-286011 exerts its inhibitory action at the PAF-receptor level. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 2115518-5 1990 In contrast, if the differentiated HL-60 cells were supplemented with 10 microM arachidonic acid for 24 h and then stimulated with the ionophore, there was a large amount of [3H]PAF formed. Tritium 175-177 PCNA clamp associated factor Homo sapiens 178-181 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 108-110 PCNA clamp associated factor Homo sapiens 111-114 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-3 1990 Binding to differentiated cells reaches equilibrium within 80 min and is saturable, reversible and specific; PAF receptor antagonists WEB 2086, L-659,989, BN 52021, and kadsurenone abolish this specific [3H]PAF binding. Tritium 204-206 PCNA clamp associated factor Homo sapiens 109-112 2155218-3 1990 Binding to differentiated cells reaches equilibrium within 80 min and is saturable, reversible and specific; PAF receptor antagonists WEB 2086, L-659,989, BN 52021, and kadsurenone abolish this specific [3H]PAF binding. Tritium 204-206 PCNA clamp associated factor Homo sapiens 207-210 2155218-4 1990 In contrast, [3H]PAF uptake by undifferentiated HL-60 cells is neither saturable nor sensitive to specific receptor antagonists. Tritium 14-16 PCNA clamp associated factor Homo sapiens 17-20 2154953-7 1990 Incubations of HL-60 cells with exogenous [3H]PAF produced a similar subcellular distribution of metabolites. Tritium 43-45 PCNA clamp associated factor Homo sapiens 46-49 2246516-6 1990 When exogenous [3H]PAF was added to the two stimulated eosinophil populations subsequent analysis of the [3H]PAF metabolites by DIOL-HPLC revealed that low density eosinophils incorporated PAF into the phosphatidylcholine (PC) pool more rapidly than did normal density eosinophils or neutrophils. Tritium 16-18 PCNA clamp associated factor Homo sapiens 19-22 2154953-2 1990 Lipid extracts obtained from undifferentiated HL-60 cells incubated with [3H]alkylacetyl-G contained 2-4% of the label as [3H]PAF; several related metabolites were also detected. Tritium 74-76 PCNA clamp associated factor Homo sapiens 126-129 2154953-3 1990 The yield of [3H]PAF could be dramatically increased by pretreating the cells with either oleic acid, an activator of CTP:phosphocholine cytidylyltransferase, or phenylmethylsulfonyl fluoride, an inhibitor of PAF acetylhydrolase. Tritium 14-16 PCNA clamp associated factor Homo sapiens 17-20 2154953-4 1990 These results, together with a kinetic study of [3H]alkylacetyl-G metabolism, indicate the sequential participation of a cholinephosphotransferase for the conversion of [3H]-alkylacetyl-G to PAF and acetylhydrolase and transacylase activities in the remodeling pathway that metabolize the newly formed [3H]PAF to 1-[3H]alkyl-2-acyl(long chain)-sn-glycero-3-phosphocholine. Tritium 49-51 PCNA clamp associated factor Homo sapiens 191-194 2154953-4 1990 These results, together with a kinetic study of [3H]alkylacetyl-G metabolism, indicate the sequential participation of a cholinephosphotransferase for the conversion of [3H]-alkylacetyl-G to PAF and acetylhydrolase and transacylase activities in the remodeling pathway that metabolize the newly formed [3H]PAF to 1-[3H]alkyl-2-acyl(long chain)-sn-glycero-3-phosphocholine. Tritium 49-51 PCNA clamp associated factor Homo sapiens 306-309 2154953-4 1990 These results, together with a kinetic study of [3H]alkylacetyl-G metabolism, indicate the sequential participation of a cholinephosphotransferase for the conversion of [3H]-alkylacetyl-G to PAF and acetylhydrolase and transacylase activities in the remodeling pathway that metabolize the newly formed [3H]PAF to 1-[3H]alkyl-2-acyl(long chain)-sn-glycero-3-phosphocholine. Tritium 170-172 PCNA clamp associated factor Homo sapiens 191-194 2154953-4 1990 These results, together with a kinetic study of [3H]alkylacetyl-G metabolism, indicate the sequential participation of a cholinephosphotransferase for the conversion of [3H]-alkylacetyl-G to PAF and acetylhydrolase and transacylase activities in the remodeling pathway that metabolize the newly formed [3H]PAF to 1-[3H]alkyl-2-acyl(long chain)-sn-glycero-3-phosphocholine. Tritium 170-172 PCNA clamp associated factor Homo sapiens 306-309 2154953-6 1990 Subcellular localization analyses also indicated that the majority of newly formed [3H]PAF and a large portion of its deacetylated metabolite were associated with the plasma membrane-containing fractions, whereas most of the 1-[3H]alkyl-2-acyl(long chain)-sn-glycero-3- phosphocholine was present in the intracellular organelles. Tritium 84-86 PCNA clamp associated factor Homo sapiens 87-90 2153465-5 1990 The binding of [3H]PAF to the U937 cell membranes was slightly inhibited by the addition of PAF analogue, 3-O-Hexadecyl-2-acetyl-sn-glycerol-1-phosphorylcholine. Tritium 16-18 PCNA clamp associated factor Homo sapiens 19-22 2153465-5 1990 The binding of [3H]PAF to the U937 cell membranes was slightly inhibited by the addition of PAF analogue, 3-O-Hexadecyl-2-acetyl-sn-glycerol-1-phosphorylcholine. Tritium 16-18 PCNA clamp associated factor Homo sapiens 92-95 2153465-6 1990 U937 cell membranes showed high affinity binding sites for PAF with equilibrium dissociation constant (Kd) of 5 x 10(-9) M. The displacement of bound [3H]PAF with 500-fold excess of nonlabeled PAF was not altered suggesting that the bound [3H]PAF was not degraded during the binding. Tritium 151-153 PCNA clamp associated factor Homo sapiens 59-62 2153465-6 1990 U937 cell membranes showed high affinity binding sites for PAF with equilibrium dissociation constant (Kd) of 5 x 10(-9) M. The displacement of bound [3H]PAF with 500-fold excess of nonlabeled PAF was not altered suggesting that the bound [3H]PAF was not degraded during the binding. Tritium 151-153 PCNA clamp associated factor Homo sapiens 154-157 2153465-6 1990 U937 cell membranes showed high affinity binding sites for PAF with equilibrium dissociation constant (Kd) of 5 x 10(-9) M. The displacement of bound [3H]PAF with 500-fold excess of nonlabeled PAF was not altered suggesting that the bound [3H]PAF was not degraded during the binding. Tritium 151-153 PCNA clamp associated factor Homo sapiens 154-157 2153465-6 1990 U937 cell membranes showed high affinity binding sites for PAF with equilibrium dissociation constant (Kd) of 5 x 10(-9) M. The displacement of bound [3H]PAF with 500-fold excess of nonlabeled PAF was not altered suggesting that the bound [3H]PAF was not degraded during the binding. Tritium 151-153 PCNA clamp associated factor Homo sapiens 154-157 2153465-6 1990 U937 cell membranes showed high affinity binding sites for PAF with equilibrium dissociation constant (Kd) of 5 x 10(-9) M. The displacement of bound [3H]PAF with 500-fold excess of nonlabeled PAF was not altered suggesting that the bound [3H]PAF was not degraded during the binding. Tritium 240-242 PCNA clamp associated factor Homo sapiens 59-62 2153465-7 1990 Binding of [3H]PAF on U937 cell membranes was inhibited by PAF antagonist, 59227RP. Tritium 12-14 PCNA clamp associated factor Homo sapiens 15-18 2153465-7 1990 Binding of [3H]PAF on U937 cell membranes was inhibited by PAF antagonist, 59227RP. Tritium 12-14 PCNA clamp associated factor Homo sapiens 59-62 2164200-17 1990 Also, [3H] WEB 2086 appears to be a suitable antagonist radioligand for labelling of PAF receptors in these inflammatory cells. Tritium 7-9 PCNA clamp associated factor Homo sapiens 85-88 2322233-3 1990 A smaller amount of [3H]-PAF co-eluted with the major monomeric species of PCBP. Tritium 21-23 PCNA clamp associated factor Homo sapiens 25-28 2545786-4 1989 When [3H] AA-labeled neutrophils were exposed to PAF, the enhanced release of AA was observed with a concomitant decrease of radioactivity in phosphatidylinositol and phosphatidylcholine fractions. Tritium 6-8 PCNA clamp associated factor Homo sapiens 49-52 2478607-3 1989 PAF, in concentrations of 5 to 100 ng/ml, induced a specific, dose-dependent release of [3H]RGC from human airways in vitro (range of 15% to 120% increase above control, p less than 0.001; n = 8). Tritium 89-91 PCNA clamp associated factor Homo sapiens 0-3 2545786-8 1989 PAF raised the fluorescence of fura 2 at concentrations as low as 8 pM which reached a maximal level about 8 nM, whereas more than nM order concentrations of PAF was required for the detectable release of [3H]AA. Tritium 206-208 PCNA clamp associated factor Homo sapiens 0-3 2545786-8 1989 PAF raised the fluorescence of fura 2 at concentrations as low as 8 pM which reached a maximal level about 8 nM, whereas more than nM order concentrations of PAF was required for the detectable release of [3H]AA. Tritium 206-208 PCNA clamp associated factor Homo sapiens 158-161 2502069-0 1989 Inhibition of [3H]platelet activating factor (PAF) binding by Zn2+: a possible explanation for its specific PAF antiaggregating effects in human platelets. Tritium 15-17 PCNA clamp associated factor Homo sapiens 46-49 2502069-7 1989 Analysis of [3H]PAF binding to platelets showed that Zn2+ as well as unlabeled PAF prevented the specific binding of [3H]PAF. Tritium 13-15 PCNA clamp associated factor Homo sapiens 16-19 2502069-7 1989 Analysis of [3H]PAF binding to platelets showed that Zn2+ as well as unlabeled PAF prevented the specific binding of [3H]PAF. Tritium 13-15 PCNA clamp associated factor Homo sapiens 79-82 2502069-7 1989 Analysis of [3H]PAF binding to platelets showed that Zn2+ as well as unlabeled PAF prevented the specific binding of [3H]PAF. Tritium 13-15 PCNA clamp associated factor Homo sapiens 79-82 2502069-8 1989 The inhibition of [3H]PAF specific binding was proportional to the concentration of Zn2+ and the IC50 value was 18 +/- 2 microM against 1 nM [3H]PAF (n = 3). Tritium 19-21 PCNA clamp associated factor Homo sapiens 22-25 2502069-8 1989 The inhibition of [3H]PAF specific binding was proportional to the concentration of Zn2+ and the IC50 value was 18 +/- 2 microM against 1 nM [3H]PAF (n = 3). Tritium 19-21 PCNA clamp associated factor Homo sapiens 145-148 2502069-10 1989 However, Cd2+ and Cu2+ at high concentrations exhibited a significant inhibition of the aggregation induced by 10 nM PAF with IC50 values being five- and sevenfold higher, respectively, than the IC50 for Zn2+, and with the IC50 values for inhibition of binding of 1 nM [3H]PAF being 5 and 19 times higher, respectively, than the IC50 for Zn2+. Tritium 270-272 PCNA clamp associated factor Homo sapiens 117-120 2811869-6 1989 Using these antibodies, [3H]PAF and Protein A-Sepharose as a means of separating bound and free tracer, the feasibility of developing a quantitative RIA for PAF was demonstrated. Tritium 25-27 PCNA clamp associated factor Homo sapiens 157-160 2804200-5 1989 The PAF was measured (in fmole equivalents of synthetic PAF) by a bioassay based on the capacity of aliquots of the extracts to release [3H]-serotonin from platelets isolated from whole blood of rabbits and prelabeled with [3H]-serotonin. Tritium 137-139 PCNA clamp associated factor Homo sapiens 4-7 2804200-5 1989 The PAF was measured (in fmole equivalents of synthetic PAF) by a bioassay based on the capacity of aliquots of the extracts to release [3H]-serotonin from platelets isolated from whole blood of rabbits and prelabeled with [3H]-serotonin. Tritium 224-226 PCNA clamp associated factor Homo sapiens 4-7 2545861-9 1989 The mechanism of this effect is probably related to the capability of Ca++ and Mg++ to enhance the maximum number of [3H]PAF binding sites in whole or lysed PMNs without affecting the affinity (Kd) of the ligand. Tritium 118-120 PCNA clamp associated factor Homo sapiens 121-124 2502069-0 1989 Inhibition of [3H]platelet activating factor (PAF) binding by Zn2+: a possible explanation for its specific PAF antiaggregating effects in human platelets. Tritium 15-17 PCNA clamp associated factor Homo sapiens 108-111 2715186-4 1989 Intact endothelial monolayers effectively shielded the underlying smooth muscle cells from PAF present in the apical fluid; after a 30-min incubation with [3H]-PAF, only 1% of the radioactivity was transferred to the interstitial fluid. Tritium 156-158 PCNA clamp associated factor Homo sapiens 160-163 2540238-2 1989 We examined the effect of priming PMN with GM-CSF on the production of [3H] platelet-activating factor (PAF) from [3H]acetate upon stimulation with the chemotactic factors FMLP and C5a. Tritium 72-74 PCNA clamp associated factor Homo sapiens 76-102 2540238-2 1989 We examined the effect of priming PMN with GM-CSF on the production of [3H] platelet-activating factor (PAF) from [3H]acetate upon stimulation with the chemotactic factors FMLP and C5a. Tritium 72-74 PCNA clamp associated factor Homo sapiens 104-107 2540238-3 1989 In PMN stimulated with the individual peptide mediators alone [3H]PAF levels were close to controls, whereas considerable amounts of [3H]PAF are formed after stimulation of PMN which have been preexposed to GM-CSF. Tritium 63-65 PCNA clamp associated factor Homo sapiens 66-69 2540238-3 1989 In PMN stimulated with the individual peptide mediators alone [3H]PAF levels were close to controls, whereas considerable amounts of [3H]PAF are formed after stimulation of PMN which have been preexposed to GM-CSF. Tritium 134-136 PCNA clamp associated factor Homo sapiens 137-140 2540238-5 1989 It was optimal after a preincubation period of 2 h. A maximum of [3H]PAF accumulation is reached within 2.5 min (C5a) and 5.0 min (FMLP) after activation of GM-CSF-primed PMN. Tritium 66-68 PCNA clamp associated factor Homo sapiens 69-72 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 23-25 PCNA clamp associated factor Homo sapiens 26-29 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 23-25 PCNA clamp associated factor Homo sapiens 48-51 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 23-25 PCNA clamp associated factor Homo sapiens 48-51 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 45-47 PCNA clamp associated factor Homo sapiens 26-29 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 45-47 PCNA clamp associated factor Homo sapiens 48-51 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 45-47 PCNA clamp associated factor Homo sapiens 48-51 2715186-8 1989 PAF enhanced the proliferative effect of PDGF on smooth muscle cells, as assessed by [3H]-thymidine incorporation. Tritium 86-88 PCNA clamp associated factor Homo sapiens 0-3 3144970-2 1988 3H-labeled PAF was shown to interact in the perfused liver primarily with Kupffer cells. Tritium 0-2 PCNA clamp associated factor Homo sapiens 11-14 3478712-4 1987 Within 2 min of stimulation of neutrophils with 10(-6) M PAF, the 7-fold increase in acetyltransferase activity was coincident with substantial PAF synthesis (as measured by [3H]acetate incorporation into PAF), which was 14% of the PAF synthesis induced by the Ca2+ ionophore A23187 at 10(-5) M. PAF activation of the acetyltransferase and PAF synthesis required intact neutrophils as they did not occur in cells broken by sonication. Tritium 175-177 PCNA clamp associated factor Homo sapiens 57-60 2846326-4 1988 In the presence of PCMBS (0.1 mM) or NEM (20 mM), the ability of PAF to displace bound [3H]PAF from neutrophil membranes was reduced. Tritium 88-90 PCNA clamp associated factor Homo sapiens 65-68 2846326-4 1988 In the presence of PCMBS (0.1 mM) or NEM (20 mM), the ability of PAF to displace bound [3H]PAF from neutrophil membranes was reduced. Tritium 88-90 PCNA clamp associated factor Homo sapiens 91-94 2831903-1 1988 The existence of specific binding sites for [3H]-labelled PAF ([3H]PAF) was investigated on membrane preparations of gerbil brain. Tritium 45-47 PCNA clamp associated factor Homo sapiens 58-61 2831903-1 1988 The existence of specific binding sites for [3H]-labelled PAF ([3H]PAF) was investigated on membrane preparations of gerbil brain. Tritium 45-47 PCNA clamp associated factor Homo sapiens 67-70 2831903-1 1988 The existence of specific binding sites for [3H]-labelled PAF ([3H]PAF) was investigated on membrane preparations of gerbil brain. Tritium 64-66 PCNA clamp associated factor Homo sapiens 58-61 2831903-1 1988 The existence of specific binding sites for [3H]-labelled PAF ([3H]PAF) was investigated on membrane preparations of gerbil brain. Tritium 64-66 PCNA clamp associated factor Homo sapiens 67-70 2831903-2 1988 Binding assays of [3H]PAF showed a specific, saturable, reversible and time dependent binding. Tritium 19-21 PCNA clamp associated factor Homo sapiens 22-25 2831903-4 1988 The binding of [3H]PAF was fully displaced by unlabelled PAF and partially inhibited by the PAF antagonist BN 52021 suggesting that BN 52021 interacts only with one site. Tritium 16-18 PCNA clamp associated factor Homo sapiens 19-22 2831903-4 1988 The binding of [3H]PAF was fully displaced by unlabelled PAF and partially inhibited by the PAF antagonist BN 52021 suggesting that BN 52021 interacts only with one site. Tritium 16-18 PCNA clamp associated factor Homo sapiens 57-60 2831903-4 1988 The binding of [3H]PAF was fully displaced by unlabelled PAF and partially inhibited by the PAF antagonist BN 52021 suggesting that BN 52021 interacts only with one site. Tritium 16-18 PCNA clamp associated factor Homo sapiens 57-60 2831903-5 1988 Distribution of [3H]PAF specific binding revealed a maximum amount of binding in midbrain and hippocampus. Tritium 17-19 PCNA clamp associated factor Homo sapiens 20-23 2830276-5 1988 Mg2+, Ca2+, and K+ ions potentiated the specific [3H]PAF binding in both systems. Tritium 50-52 PCNA clamp associated factor Homo sapiens 53-56 2830276-7 1988 K+ ions decreased the Mg2+-potentiated [3H]PAF binding in human leukocytes but showed no effects in human platelets. Tritium 40-42 PCNA clamp associated factor Homo sapiens 43-46 2833227-3 1988 Similarly when PAF was added to [3H]-glycerol prelabelled macrophages there was a rapid increase in 1,2-diacyl[3H]glycerol levels. Tritium 33-35 PCNA clamp associated factor Homo sapiens 15-18 2833227-5 1988 Increasing concentrations of PAF led to a markedly similar increase in both ROI production and [3H]inositol lipid hydrolysis suggesting that inositol lipid hydrolysis may lead to the generation of ROI in macrophages. Tritium 96-98 PCNA clamp associated factor Homo sapiens 29-32 2853256-1 1988 High-affinity, stereoselective, and ligand-selective specific binding of 3H-labeled platelet-activating factor (PAF) to its receptor on the dog platelet is reproducible over wide mass and concentration ranges of [3H]PAF. Tritium 73-75 PCNA clamp associated factor Homo sapiens 112-115 2853256-1 1988 High-affinity, stereoselective, and ligand-selective specific binding of 3H-labeled platelet-activating factor (PAF) to its receptor on the dog platelet is reproducible over wide mass and concentration ranges of [3H]PAF. Tritium 73-75 PCNA clamp associated factor Homo sapiens 216-219 2853256-1 1988 High-affinity, stereoselective, and ligand-selective specific binding of 3H-labeled platelet-activating factor (PAF) to its receptor on the dog platelet is reproducible over wide mass and concentration ranges of [3H]PAF. Tritium 213-215 PCNA clamp associated factor Homo sapiens 112-115 2853256-2 1988 The [3H]PAF specific binding can be competitively inhibited by low picogram amounts of nonlabeled PAF. Tritium 5-7 PCNA clamp associated factor Homo sapiens 8-11 2853256-2 1988 The [3H]PAF specific binding can be competitively inhibited by low picogram amounts of nonlabeled PAF. Tritium 5-7 PCNA clamp associated factor Homo sapiens 98-101 2853256-4 1988 The methodology is predicated upon correlation between the ability of a PAF analog/biological lipid sample/(synthetic) substance to inhibit [3H]PAF specific binding to the washed canine platelet and the known inhibition of [3H]PAF specific binding by standard, nonradioactive PAF. Tritium 141-143 PCNA clamp associated factor Homo sapiens 72-75 2853256-4 1988 The methodology is predicated upon correlation between the ability of a PAF analog/biological lipid sample/(synthetic) substance to inhibit [3H]PAF specific binding to the washed canine platelet and the known inhibition of [3H]PAF specific binding by standard, nonradioactive PAF. Tritium 141-143 PCNA clamp associated factor Homo sapiens 144-147 2853256-4 1988 The methodology is predicated upon correlation between the ability of a PAF analog/biological lipid sample/(synthetic) substance to inhibit [3H]PAF specific binding to the washed canine platelet and the known inhibition of [3H]PAF specific binding by standard, nonradioactive PAF. Tritium 141-143 PCNA clamp associated factor Homo sapiens 144-147 2853256-4 1988 The methodology is predicated upon correlation between the ability of a PAF analog/biological lipid sample/(synthetic) substance to inhibit [3H]PAF specific binding to the washed canine platelet and the known inhibition of [3H]PAF specific binding by standard, nonradioactive PAF. Tritium 141-143 PCNA clamp associated factor Homo sapiens 144-147 2853256-4 1988 The methodology is predicated upon correlation between the ability of a PAF analog/biological lipid sample/(synthetic) substance to inhibit [3H]PAF specific binding to the washed canine platelet and the known inhibition of [3H]PAF specific binding by standard, nonradioactive PAF. Tritium 224-226 PCNA clamp associated factor Homo sapiens 72-75 3342883-4 1988 15-fold higher than for [3H]PAF and [3H]WEB 2086. Tritium 25-27 PCNA clamp associated factor Homo sapiens 28-31 3342883-5 1988 In addition, C16-PAF, lyso-PAF, WEB 2086 and 52770 RP had Ki values which were nearly identical for both [3H]PAF and [3H]WEB 2086, whereas only 52770 RP competed for [3H]52770 RP-binding sites. Tritium 106-108 PCNA clamp associated factor Homo sapiens 17-20 3342883-5 1988 In addition, C16-PAF, lyso-PAF, WEB 2086 and 52770 RP had Ki values which were nearly identical for both [3H]PAF and [3H]WEB 2086, whereas only 52770 RP competed for [3H]52770 RP-binding sites. Tritium 106-108 PCNA clamp associated factor Homo sapiens 27-30 3342883-5 1988 In addition, C16-PAF, lyso-PAF, WEB 2086 and 52770 RP had Ki values which were nearly identical for both [3H]PAF and [3H]WEB 2086, whereas only 52770 RP competed for [3H]52770 RP-binding sites. Tritium 118-120 PCNA clamp associated factor Homo sapiens 17-20 3342883-5 1988 In addition, C16-PAF, lyso-PAF, WEB 2086 and 52770 RP had Ki values which were nearly identical for both [3H]PAF and [3H]WEB 2086, whereas only 52770 RP competed for [3H]52770 RP-binding sites. Tritium 118-120 PCNA clamp associated factor Homo sapiens 27-30 3342883-5 1988 In addition, C16-PAF, lyso-PAF, WEB 2086 and 52770 RP had Ki values which were nearly identical for both [3H]PAF and [3H]WEB 2086, whereas only 52770 RP competed for [3H]52770 RP-binding sites. Tritium 118-120 PCNA clamp associated factor Homo sapiens 17-20 3342883-5 1988 In addition, C16-PAF, lyso-PAF, WEB 2086 and 52770 RP had Ki values which were nearly identical for both [3H]PAF and [3H]WEB 2086, whereas only 52770 RP competed for [3H]52770 RP-binding sites. Tritium 118-120 PCNA clamp associated factor Homo sapiens 27-30 3342883-7 1988 [3H]WEB 2086 appears, therefore, to be a suitable antagonist radioligand for labelling PAF receptors. Tritium 1-3 PCNA clamp associated factor Homo sapiens 87-90 2825685-0 1987 Inhibition by BN 52021 (ginkgolide B) of the binding of [3H]-platelet-activating factor to human neutrophil granulocytes. Tritium 57-59 PCNA clamp associated factor Homo sapiens 61-87 2825685-4 1987 The Ki value of BN 52021 for the specific binding of [3H]-PAF to neutrophils was 1.3 +/- 0.5 x 10(-6) M versus a Ki of 1.1 +/- 0.3 x 10(-7) M for PAF itself. Tritium 54-56 PCNA clamp associated factor Homo sapiens 58-61 2820395-1 1987 We prepared unlabeled and 3H-labeled analogs of platelet-activating factor (PAF) containing a N-methylcarbamyl residue at the sn-2 position. Tritium 26-28 PCNA clamp associated factor Homo sapiens 48-74 2820395-1 1987 We prepared unlabeled and 3H-labeled analogs of platelet-activating factor (PAF) containing a N-methylcarbamyl residue at the sn-2 position. Tritium 26-28 PCNA clamp associated factor Homo sapiens 76-79 3612533-3 1987 CV-6209 inhibited [3H]serotonin release from rabbit platelets stimulated with PAF (3 X 10(-8) M) with a similar potency as the inhibition on the platelet aggregation. Tritium 19-21 PCNA clamp associated factor Homo sapiens 78-81 6510472-1 1984 The binding of platelet-activating factor (PAF-acether) to human, rabbit and rat platelets was investigated by incubating washed platelets at 37 degrees C with radiolabeled [3H]PAF-acether. Tritium 174-176 PCNA clamp associated factor Homo sapiens 43-46 2986648-5 1985 CV-3988 also inhibited the specific binding of [3H]-PAF to human and guinea-pig platelets with IC50 values of 1.6 X 10(-7) and 1.8 X 10(-7) M, respectively. Tritium 48-50 PCNA clamp associated factor Homo sapiens 52-55 6150705-1 1984 The binding of [3H]PAF-acether (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) to intact human gel-filtered platelets was measured at 22 degrees C. Specific binding reached saturation within 15 min at high doses of [3H]PAF-acether (0.5-0.9 nM), whereas about 90 min were required when low doses (0.02-0.5 nM) were used. Tritium 16-18 PCNA clamp associated factor Homo sapiens 19-22 6150705-1 1984 The binding of [3H]PAF-acether (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) to intact human gel-filtered platelets was measured at 22 degrees C. Specific binding reached saturation within 15 min at high doses of [3H]PAF-acether (0.5-0.9 nM), whereas about 90 min were required when low doses (0.02-0.5 nM) were used. Tritium 16-18 PCNA clamp associated factor Homo sapiens 220-223 6150705-2 1984 Above 1 nM, [3H]PAF-acether non-specific binding increased progressively, which together with the demonstration of a 3H-labelled metabolite suggested uptake and metabolism of [3H]PAF-acether. Tritium 13-15 PCNA clamp associated factor Homo sapiens 16-19 6150705-2 1984 Above 1 nM, [3H]PAF-acether non-specific binding increased progressively, which together with the demonstration of a 3H-labelled metabolite suggested uptake and metabolism of [3H]PAF-acether. Tritium 13-15 PCNA clamp associated factor Homo sapiens 179-182 4063532-6 1985 The assay for PAF acetylhydrolase was based on the release of 3H from PAF that was labelled in the acetate moiety of the sn-2 position. Tritium 62-64 PCNA clamp associated factor Homo sapiens 14-17 3001971-2 1985 The final preparation migrated as a single protein band corresponding to a radioactive peak of [3H]PAF bound on polyacrylamide disc gel electrophoresis. Tritium 96-98 PCNA clamp associated factor Homo sapiens 99-102 3001971-4 1985 Exposure of the platelet membranes to an excess of unlabeled PAF diminished the binding of [3H]PAF. Tritium 92-94 PCNA clamp associated factor Homo sapiens 61-64 3001971-4 1985 Exposure of the platelet membranes to an excess of unlabeled PAF diminished the binding of [3H]PAF. Tritium 92-94 PCNA clamp associated factor Homo sapiens 95-98 6510472-5 1984 Addition of unlabeled PAF-acether 5 min after the addition of [3H]PAF-acether showed that internalization of 66 +/- 9% of the specific binding in human and 52 +/- 6.9% in rabbit platelets had occurred. Tritium 63-65 PCNA clamp associated factor Homo sapiens 22-25 6510472-5 1984 Addition of unlabeled PAF-acether 5 min after the addition of [3H]PAF-acether showed that internalization of 66 +/- 9% of the specific binding in human and 52 +/- 6.9% in rabbit platelets had occurred. Tritium 63-65 PCNA clamp associated factor Homo sapiens 66-69 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 21-23 PCNA clamp associated factor Homo sapiens 24-27 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 21-23 PCNA clamp associated factor Homo sapiens 133-136 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 78-80 PCNA clamp associated factor Homo sapiens 24-27 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 78-80 PCNA clamp associated factor Homo sapiens 133-136 6481248-3 1984 This peak was well separated from other classes of phospholipid standards including 2-lysophosphatidylcholine and 3H-labeled lyso-PAF. Tritium 114-116 PCNA clamp associated factor Homo sapiens 130-133