PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1443598-1 1992 A rapid quantitative analysis of the sialylated N-linked oligosaccharides of recombinant erythropoietin (EPO) expressed in Chinese hamster ovary (CHO) cells has been developed. n-linked oligosaccharides 48-73 erythropoietin Cricetulus griseus 89-103 8717231-4 1995 The N-linked oligosaccharides were released from EPO by the treatment with N-glycosidase F. HPAEC analysis of oligosaccharide standards revealed that elution time of sialylated oligosaccharides were dependent on the number of sialic acid, which contributed to the activity of EPO. n-linked oligosaccharides 4-29 erythropoietin Cricetulus griseus 49-52 8717231-4 1995 The N-linked oligosaccharides were released from EPO by the treatment with N-glycosidase F. HPAEC analysis of oligosaccharide standards revealed that elution time of sialylated oligosaccharides were dependent on the number of sialic acid, which contributed to the activity of EPO. n-linked oligosaccharides 4-29 erythropoietin Cricetulus griseus 276-279 1443598-1 1992 A rapid quantitative analysis of the sialylated N-linked oligosaccharides of recombinant erythropoietin (EPO) expressed in Chinese hamster ovary (CHO) cells has been developed. n-linked oligosaccharides 48-73 erythropoietin Cricetulus griseus 105-108 2043765-8 1991 However, EPO produced with incompletely processed N-linked oligosaccharides exhibits normal in vitro activity but is at least 500-fold less effective in stimulating erythropoiesis in vivo. n-linked oligosaccharides 50-75 erythropoietin Cricetulus griseus 9-12 2043765-9 1991 Studies on the survival of bioactive EPO remaining in the circulation demonstrated that EPO with incomplete N-linked oligosaccharides exhibits a sevenfold increased rate of clearance. n-linked oligosaccharides 108-133 erythropoietin Cricetulus griseus 88-91 2043765-11 1991 These results suggest a potentially important unique requirement for appropriate complex N-linked oligosaccharides for the intrinsic biologic activity of EPO in vivo. n-linked oligosaccharides 89-114 erythropoietin Cricetulus griseus 154-157