PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
11606251-4	2001	The F-actin in permeabilised and myosin free rabbit skeletal muscle "ghost" fibres was labelled by tetramethyl rhodamine-isothiocyanate (TRITC)-phalloidin or fluorescein-5"-isothiocyanate (FITC) at lysine 61.	Fluorescein-5-isothiocyanate	158-187	actin	Oryctolagus cuniculus	6-11	
6437449-0	1984	Modification of actin with fluorescein isothiocyanate.	Fluorescein-5-isothiocyanate	27-53	actin	Oryctolagus cuniculus	16-21	
6437449-1	1984	Reaction of rabbit skeletal muscle G-actin at pH 8.5 with fluorescein isothiocyanate (FITC) resulted in incorporation of up to 1.20 mol FITC/mol actin.	Fluorescein-5-isothiocyanate	58-84	actin	Oryctolagus cuniculus	37-42	
6437449-1	1984	Reaction of rabbit skeletal muscle G-actin at pH 8.5 with fluorescein isothiocyanate (FITC) resulted in incorporation of up to 1.20 mol FITC/mol actin.	Fluorescein-5-isothiocyanate	58-84	actin	Oryctolagus cuniculus	145-150	
6437449-1	1984	Reaction of rabbit skeletal muscle G-actin at pH 8.5 with fluorescein isothiocyanate (FITC) resulted in incorporation of up to 1.20 mol FITC/mol actin.	Fluorescein-5-isothiocyanate	86-90	actin	Oryctolagus cuniculus	37-42	
6437449-1	1984	Reaction of rabbit skeletal muscle G-actin at pH 8.5 with fluorescein isothiocyanate (FITC) resulted in incorporation of up to 1.20 mol FITC/mol actin.	Fluorescein-5-isothiocyanate	86-90	actin	Oryctolagus cuniculus	145-150	
6437449-1	1984	Reaction of rabbit skeletal muscle G-actin at pH 8.5 with fluorescein isothiocyanate (FITC) resulted in incorporation of up to 1.20 mol FITC/mol actin.	Fluorescein-5-isothiocyanate	136-140	actin	Oryctolagus cuniculus	37-42	
6437449-1	1984	Reaction of rabbit skeletal muscle G-actin at pH 8.5 with fluorescein isothiocyanate (FITC) resulted in incorporation of up to 1.20 mol FITC/mol actin.	Fluorescein-5-isothiocyanate	136-140	actin	Oryctolagus cuniculus	145-150	
6437449-2	1984	At pH 8.8, the level of incorporation was raised to 1.98 mol FITC/mol actin.	Fluorescein-5-isothiocyanate	61-65	actin	Oryctolagus cuniculus	70-75	
6437449-3	1984	When excited with ultraviolet light, the FITC-actin samples fluoresced strongly with an emission maximum near 517 nm.	Fluorescein-5-isothiocyanate	41-45	actin	Oryctolagus cuniculus	46-51	
6437449-4	1984	Tryptic digests of FITC-actin containing about 1.0 mol FITC/mol actin could be separated into a nonfluorescent 33.5 kDa trypsin-resistant core protein and a fluorescent pool of small peptides.	Fluorescein-5-isothiocyanate	19-23	actin	Oryctolagus cuniculus	24-29	
6437449-4	1984	Tryptic digests of FITC-actin containing about 1.0 mol FITC/mol actin could be separated into a nonfluorescent 33.5 kDa trypsin-resistant core protein and a fluorescent pool of small peptides.	Fluorescein-5-isothiocyanate	19-23	actin	Oryctolagus cuniculus	64-69	
6437449-4	1984	Tryptic digests of FITC-actin containing about 1.0 mol FITC/mol actin could be separated into a nonfluorescent 33.5 kDa trypsin-resistant core protein and a fluorescent pool of small peptides.	Fluorescein-5-isothiocyanate	55-59	actin	Oryctolagus cuniculus	24-29	
6437449-5	1984	Chromatography on DEAE-Bio-Gel or two-dimensional separation on cellulose TLC plates of the peptide pool revealed that FITC was highly selective in the site of its reaction with actin, resulting in a single highly fluorescent peptide after tryptic digestion.	Fluorescein-5-isothiocyanate	119-123	actin	Oryctolagus cuniculus	178-183	
6437449-6	1984	NH2-terminal and amino acid analyses demonstrated this peptide to be derived from residues 51 to 62, with Lys-61 proposed as the major FITC-sensitive site on actin.	Fluorescein-5-isothiocyanate	135-139	actin	Oryctolagus cuniculus	158-163	
6437449-7	1984	FITC-actin is similar to G-actin in gross conformation; circular dichroism spectra of actin before and after labelling are identical.	Fluorescein-5-isothiocyanate	0-4	actin	Oryctolagus cuniculus	5-10	
6437449-8	1984	FITC-actin is also able to interact strongly with deoxyribonuclease I.	Fluorescein-5-isothiocyanate	0-4	actin	Oryctolagus cuniculus	5-10	
6437449-9	1984	However, FITC-actin solution viscosities and fluorescence properties are not altered by the addition of KCl or MgCl2.	Fluorescein-5-isothiocyanate	9-13	actin	Oryctolagus cuniculus	14-19	
6437449-10	1984	Therefore, either a localized conformational change near Lys-61 or steric hindrance due to the FITC attached to Lys-61 blocks the polymerization of actin.	Fluorescein-5-isothiocyanate	95-99	actin	Oryctolagus cuniculus	148-153