PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7969079-3 1994 Using melphalan-resistant DU 145/M4.5 human prostate carcinoma cells, which express elevated GSH levels, GCS enzyme activity, and GCS mRNA levels, we sought to determine the mechanism(s) responsible for the increased GCS mRNA expression. Melphalan 6-15 glutamate-cysteine ligase catalytic subunit Homo sapiens 105-108 7585502-5 1995 A direct correlation (P < 0.01) between intracellular GSH levels and the LD99 dose of melphalan was observed, signifying that elevation of the thiol secondary to GCS expression is sufficient to confer the resistance phenotype. Melphalan 89-98 glutamate-cysteine ligase catalytic subunit Homo sapiens 165-168 7969079-3 1994 Using melphalan-resistant DU 145/M4.5 human prostate carcinoma cells, which express elevated GSH levels, GCS enzyme activity, and GCS mRNA levels, we sought to determine the mechanism(s) responsible for the increased GCS mRNA expression. Melphalan 6-15 glutamate-cysteine ligase catalytic subunit Homo sapiens 130-133 7969079-3 1994 Using melphalan-resistant DU 145/M4.5 human prostate carcinoma cells, which express elevated GSH levels, GCS enzyme activity, and GCS mRNA levels, we sought to determine the mechanism(s) responsible for the increased GCS mRNA expression. Melphalan 6-15 glutamate-cysteine ligase catalytic subunit Homo sapiens 130-133 7513621-1 1994 Levels of intracellular glutathione (GSH) and the GSH-related enzymes gamma-glutamylcysteine synthetase (gamma-GCS) and gamma-glutamyltranspeptidase (gamma-GT) were measured in the melphalan-resistant human multiple myeloma cell line 8226/LR-5 and were compared to those measured in the drug-sensitive 8226/S and doxorubicin-resistant 8226/Dox40 cell lines. Melphalan 181-190 glutamate-cysteine ligase catalytic subunit Homo sapiens 70-103 7513621-2 1994 Both GSH and gamma-GCS activity, the rate-limiting step in the de novo synthesis of GSH, were elevated by a factor of approximately 2 in the melphalan-resistant 8226/LR-5 cells relative to the other two lines. Melphalan 141-150 glutamate-cysteine ligase catalytic subunit Homo sapiens 13-22