PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30899446-1 2019 P-glycoprotein (P-gp) is a multidrug transporter, which harnesses the chemical energy of ATP to power the efflux of diverse chemotherapeutics out of cells and thus contributes to the development of multidrug resistance (MDR) in cancer. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 30899446-1 2019 P-glycoprotein (P-gp) is a multidrug transporter, which harnesses the chemical energy of ATP to power the efflux of diverse chemotherapeutics out of cells and thus contributes to the development of multidrug resistance (MDR) in cancer. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 31223218-2 2018 Over expression of a range of ATP-dependent efflux pumps, particularly ABCB1 is a widely reported mechanism of cancer cell MDR. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 71-76 32254552-7 2019 We showed that the nanohybrids possessed good colloidal stability and enabled significant inhibition of cancer cells due to the presence of TPGS, which can inhibit P-glycoprotein (P-gp) by decreasing ATP levels and increasing ROS levels; simultaneously, fluorescence imaging of cancer cells could be achieved in vitro due to the luminescence of CDs. Adenosine Triphosphate 200-203 ATP binding cassette subfamily B member 1 Homo sapiens 180-184 30765569-4 2019 Minor structural differences between substrate- and inhibitor-bound ABCB1 sites are amplified toward the nucleotide-binding domains (NBDs), revealing how the plasticity of the drug-binding site controls the dynamics of the adenosine triphosphate-hydrolyzing NBDs. Adenosine Triphosphate 223-245 ATP binding cassette subfamily B member 1 Homo sapiens 68-73 30390439-2 2019 MDR is often associated with the overexpression of ABC transport proteins like ABCB1 or ABCG2 which efflux harmful substances out of cells at the cost of ATP hydrolysis. Adenosine Triphosphate 154-157 ATP binding cassette subfamily B member 1 Homo sapiens 79-84 30279588-3 2018 Following drug binding, inward-facing Pgp must transition to an NBD dimer conformation to achieve ATP binding and hydrolysis at canonical sites defined by both halves of the interface. Adenosine Triphosphate 98-101 ATP binding cassette subfamily B member 1 Homo sapiens 38-41 30279588-8 2018 Apo-Pgp reached a pseudo-dimerization in which NBD signature motifs for ATP binding exhibited a significant misalignment during closure. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 4-7 29723928-1 2018 P-glycoprotein, encoded by ABCB1, is an ATP-dependent drug efflux pump which exports substances outside the cell. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 30412370-3 2018 A member of the adenosine triphosphate (ATP)-binding cassette family, P-glycoprotein, encoded by the human ABCB1 gene, is among the most extensively studied transporters involved in drug disposition and effects. Adenosine Triphosphate 16-38 ATP binding cassette subfamily B member 1 Homo sapiens 70-84 30412370-3 2018 A member of the adenosine triphosphate (ATP)-binding cassette family, P-glycoprotein, encoded by the human ABCB1 gene, is among the most extensively studied transporters involved in drug disposition and effects. Adenosine Triphosphate 16-38 ATP binding cassette subfamily B member 1 Homo sapiens 107-112 30412370-3 2018 A member of the adenosine triphosphate (ATP)-binding cassette family, P-glycoprotein, encoded by the human ABCB1 gene, is among the most extensively studied transporters involved in drug disposition and effects. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 70-84 30412370-3 2018 A member of the adenosine triphosphate (ATP)-binding cassette family, P-glycoprotein, encoded by the human ABCB1 gene, is among the most extensively studied transporters involved in drug disposition and effects. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 107-112 29723928-1 2018 P-glycoprotein, encoded by ABCB1, is an ATP-dependent drug efflux pump which exports substances outside the cell. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 27-32 30121251-1 2018 The membrane-bound P-glycoprotein (Pgp) transporter plays a major role in human disease and drug disposition because of its ability to efflux a chemically diverse range of drugs through ATP hydrolysis and ligand-induced conformational changes. Adenosine Triphosphate 186-189 ATP binding cassette subfamily B member 1 Homo sapiens 19-33 30121251-1 2018 The membrane-bound P-glycoprotein (Pgp) transporter plays a major role in human disease and drug disposition because of its ability to efflux a chemically diverse range of drugs through ATP hydrolysis and ligand-induced conformational changes. Adenosine Triphosphate 186-189 ATP binding cassette subfamily B member 1 Homo sapiens 35-38 29499278-3 2018 In addition to these differences, they showed significant differences in their effects on Pgp-mediated adenosine triphosphate (ATP) hydrolysis. Adenosine Triphosphate 103-125 ATP binding cassette subfamily B member 1 Homo sapiens 90-93 30037151-1 2018 P-glycoprotein (P-gp), a membrane-bound transporter, can eliminate xenobiotics by transporting them out of the cells or blood-brain barrier (BBB) at the expense of ATP hydrolysis. Adenosine Triphosphate 164-167 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 30037151-1 2018 P-glycoprotein (P-gp), a membrane-bound transporter, can eliminate xenobiotics by transporting them out of the cells or blood-brain barrier (BBB) at the expense of ATP hydrolysis. Adenosine Triphosphate 164-167 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 29976562-9 2018 Cryoelectron microscopy structural and homology models of MRP1 indicate that the orientation of the Phe583 side chain is altered by ATP binding but are currently unable to provide insights into the molecular mechanism by which this long-range signaling is propagated. Adenosine Triphosphate 132-135 ATP binding cassette subfamily B member 1 Homo sapiens 58-62 30143707-1 2018 P-glycoprotein (P-gp), an ATP-dependent efflux pump, is associated with the development of multidrug resistance in cancer cells. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 30143707-1 2018 P-glycoprotein (P-gp), an ATP-dependent efflux pump, is associated with the development of multidrug resistance in cancer cells. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 29499278-3 2018 In addition to these differences, they showed significant differences in their effects on Pgp-mediated adenosine triphosphate (ATP) hydrolysis. Adenosine Triphosphate 127-130 ATP binding cassette subfamily B member 1 Homo sapiens 90-93 29499278-4 2018 The activation of Pgp-mediated ATP hydrolysis by methadone was monophasic, whereas loperamide activation of ATP hydrolysis was biphasic implying methadone has a single binding site and loperamide has 2 binding sites on Pgp. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 18-21 29290467-0 2018 ATP Binding Enables Substrate Release from Multidrug Resistance Protein 1. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 43-73 29484528-2 2018 We engineer a cell line to over-express the multi-drug resistance 1 protein (MDR1), an ATP-dependent pump that effluxes intracellular drug. Adenosine Triphosphate 87-90 ATP binding cassette subfamily B member 1 Homo sapiens 44-67 29484528-2 2018 We engineer a cell line to over-express the multi-drug resistance 1 protein (MDR1), an ATP-dependent pump that effluxes intracellular drug. Adenosine Triphosphate 87-90 ATP binding cassette subfamily B member 1 Homo sapiens 77-81 29377455-3 2018 The ATP-driven efflux transporter P-glycoprotein (P-gp) is expressed in normal and malignant tissues and extrudes structurally distinct endogenous and exogenous cytotoxic compounds. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 34-48 29377455-3 2018 The ATP-driven efflux transporter P-glycoprotein (P-gp) is expressed in normal and malignant tissues and extrudes structurally distinct endogenous and exogenous cytotoxic compounds. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 50-54 29385210-1 2018 ATP Binding Cassette family efflux proteins ABCB1 and ABCG2 have previously been demonstrated to interact with Tyrosine Kinase Inhibitors (TKIs); however, evidence for the interaction of other potentially relevant drug transporters with TKIs is lacking. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 44-49 29401431-2 2018 ABCB1 consists of two transmembrane domains that form the substrate binding and translocation domain, and of two cytoplasmic nucleotide binding domains (NBDs) that energize substrate transport by ATP binding and hydrolysis. Adenosine Triphosphate 196-199 ATP binding cassette subfamily B member 1 Homo sapiens 0-5 29576448-3 2018 MDR1 gene is an ATP-dependent drug efflux pump known to be a risk factor for the emergence of resistance, when combined to unstable cytogenetic profile of AML patients. Adenosine Triphosphate 16-19 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 29511086-2 2018 P-gp utilizes ATP hydrolysis by nucleotide-binding domains (NBDs) to drive transitions between inward-facing (IF) conformations that bind drugs and outward-facing (OF) conformations that release them to the extracellular solution. Adenosine Triphosphate 14-17 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 29371429-0 2018 Molecular structure of human P-glycoprotein in the ATP-bound, outward-facing conformation. Adenosine Triphosphate 51-54 ATP binding cassette subfamily B member 1 Homo sapiens 29-43 29371429-2 2018 P-glycoprotein extrudes toxic molecules and drugs from cells through ATP-powered conformational changes. Adenosine Triphosphate 69-72 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 26932198-3 2016 P-glycoprotein (P-gp) belongs to the family of adenosine triphosphate (ATP)-binding cassette (ABC) transporters. Adenosine Triphosphate 47-69 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 29018094-0 2017 Substrate-induced conformational changes in the nucleotide-binding domains of lipid bilayer-associated P-glycoprotein during ATP hydrolysis. Adenosine Triphosphate 125-128 ATP binding cassette subfamily B member 1 Homo sapiens 103-117 29018094-2 2017 Pgp is a prototype ATP-binding cassette transporter with two nucleotide-binding domains (NBDs) that bind and hydrolyze ATP. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 29018094-7 2017 Pgp was labeled with LRET probes, reconstituted in lipid nanodiscs, and the distance between the NBDs was measured at 37 C. In the presence of verapamil, a substrate that activates ATP hydrolysis, the NBDs of Pgp reconstituted in nanodiscs were never far apart during the hydrolysis cycle, and we never observed the NBD-NBD distances of tens of A that have previously been reported. Adenosine Triphosphate 182-185 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 29077148-8 2017 Immunohistochemistry staining was used to detect the expression of P-gp (P-glycoprotein), an ATP-dependent efflux pump that mediates MDR in xenograft tumor tissues. Adenosine Triphosphate 93-96 ATP binding cassette subfamily B member 1 Homo sapiens 67-71 29077148-8 2017 Immunohistochemistry staining was used to detect the expression of P-gp (P-glycoprotein), an ATP-dependent efflux pump that mediates MDR in xenograft tumor tissues. Adenosine Triphosphate 93-96 ATP binding cassette subfamily B member 1 Homo sapiens 73-87 28915956-2 2017 ATP-dependent drug efflux pumps, including P-glycoprotein (P-gp), contribute to the resistance of various chemotherapeutic agents. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 43-57 28915956-2 2017 ATP-dependent drug efflux pumps, including P-glycoprotein (P-gp), contribute to the resistance of various chemotherapeutic agents. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 28283434-3 2017 Consistent with these transport differences, ETT activated Pgp-mediated ATP hydrolysis ~2-fold, whereas STT slightly inhibited Pgp-mediated ATP hydrolysis by ~10%. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 59-62 28283434-3 2017 Consistent with these transport differences, ETT activated Pgp-mediated ATP hydrolysis ~2-fold, whereas STT slightly inhibited Pgp-mediated ATP hydrolysis by ~10%. Adenosine Triphosphate 140-143 ATP binding cassette subfamily B member 1 Homo sapiens 127-130 28283434-7 2017 Tertiary conformational changes probed by acrylamide quenching of Pgp tryptophan fluorescence with the drugs and a nonhydrolyzable ATP analog implied that the STT-bound Pgp must undergo larger conformational changes to hydrolyze ATP than ETT-bound Pgp. Adenosine Triphosphate 131-134 ATP binding cassette subfamily B member 1 Homo sapiens 169-172 28283434-7 2017 Tertiary conformational changes probed by acrylamide quenching of Pgp tryptophan fluorescence with the drugs and a nonhydrolyzable ATP analog implied that the STT-bound Pgp must undergo larger conformational changes to hydrolyze ATP than ETT-bound Pgp. Adenosine Triphosphate 131-134 ATP binding cassette subfamily B member 1 Homo sapiens 169-172 28283434-7 2017 Tertiary conformational changes probed by acrylamide quenching of Pgp tryptophan fluorescence with the drugs and a nonhydrolyzable ATP analog implied that the STT-bound Pgp must undergo larger conformational changes to hydrolyze ATP than ETT-bound Pgp. Adenosine Triphosphate 229-232 ATP binding cassette subfamily B member 1 Homo sapiens 169-172 28283434-7 2017 Tertiary conformational changes probed by acrylamide quenching of Pgp tryptophan fluorescence with the drugs and a nonhydrolyzable ATP analog implied that the STT-bound Pgp must undergo larger conformational changes to hydrolyze ATP than ETT-bound Pgp. Adenosine Triphosphate 229-232 ATP binding cassette subfamily B member 1 Homo sapiens 169-172 28283434-8 2017 These results and previous transport studies were used to build a conformationally driven model for triptan transport with Pgp where STT presents a higher conformational barrier for ATP hydrolysis and transport than ETT. Adenosine Triphosphate 182-185 ATP binding cassette subfamily B member 1 Homo sapiens 123-126 28587082-4 2017 P-gp, a unique ATP-dependent membrane transport protein, is one of those key regulators which are present in the lining of the colon, endothelial cells of the blood brain barrier (BBB), bile duct, adrenal gland, kidney tubules, small intestine, pancreatic ducts and in many other tissues like heart, lungs, spleen, skeletal muscles, etc. Adenosine Triphosphate 15-18 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 28587082-8 2017 P-gp inhibitors act by altering intracellular ATP levels which are the source of energy and/or by affecting membrane contours to increase permeability. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 27956367-5 2017 P-gp expression level, ATP content and mitochondrial membrane potential change have been measured, the results indicated blank FA-TPGS2K/HDP mixed micelles could inhibit the P-gp activity by reducing the mitochondrial membrane potential and depleting ATP content but not down-regulating the P-gp expression. Adenosine Triphosphate 251-254 ATP binding cassette subfamily B member 1 Homo sapiens 174-178 27956367-5 2017 P-gp expression level, ATP content and mitochondrial membrane potential change have been measured, the results indicated blank FA-TPGS2K/HDP mixed micelles could inhibit the P-gp activity by reducing the mitochondrial membrane potential and depleting ATP content but not down-regulating the P-gp expression. Adenosine Triphosphate 251-254 ATP binding cassette subfamily B member 1 Homo sapiens 174-178 30497065-12 2018 Further ATP content detection and molecular docking simulations showed that NFV could decrease intracellular ATP content and has a high affinity with the active functional regions of P-gp, respectively. Adenosine Triphosphate 8-11 ATP binding cassette subfamily B member 1 Homo sapiens 183-187 29155127-4 2018 Since one of the main mechanisms of drug resistance is the removal of the drug from the cell by ATP-dependent efflux proteins, thus MDR1, single nucleotide polymorphism (SNP) C3435T can be used as a predictor for treatment outcomes. Adenosine Triphosphate 96-99 ATP binding cassette subfamily B member 1 Homo sapiens 132-136 28401939-1 2018 The multidrug resistance-1 (MDR1) gene encodes an ATP-dependent efflux transporter that is highly expressed in the colon. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 4-26 28401939-1 2018 The multidrug resistance-1 (MDR1) gene encodes an ATP-dependent efflux transporter that is highly expressed in the colon. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 28837794-1 2017 P-glycoprotein (P-gp) is an ATP-dependent drug pump that protects us from toxic agents and confers multidrug resistance. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 28837794-1 2017 P-glycoprotein (P-gp) is an ATP-dependent drug pump that protects us from toxic agents and confers multidrug resistance. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 28837794-10 2017 The results suggest that the E556 and E1201 catalytic carboxylates are part of a spring-like mechanism that is required to facilitate movements between the open and closed conformations of P-gp during ATP hydrolysis. Adenosine Triphosphate 201-204 ATP binding cassette subfamily B member 1 Homo sapiens 189-193 28728917-1 2017 P-glycoprotein (P-gp), an ATP-dependent efflux pump, is linked to the development of multidrug resistance in cancer cells. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 28728917-1 2017 P-glycoprotein (P-gp), an ATP-dependent efflux pump, is linked to the development of multidrug resistance in cancer cells. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 28728917-3 2017 We recently demonstrated the important role of tyrosine residues in regulating P-gp ATP hydrolysis via hydrogen bond formations with high affinity modulators. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 79-83 29145976-2 2017 Overexpression of a wide-spectrum of ATP-dependent efflux pumps, and in particular of ABCB1 (P-glycoprotein or MDR1) is a well-known resistance mechanism for a plethora of cancer chemotherapeutics including for example taxenes, anthracyclines, Vinca alkaloids, and epipodopyllotoxins, demonstrated by a large array of published papers, both in tumor cell lines and in a variety of tumors, including various solid tumors and hematological malignancies. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 86-91 29145976-2 2017 Overexpression of a wide-spectrum of ATP-dependent efflux pumps, and in particular of ABCB1 (P-glycoprotein or MDR1) is a well-known resistance mechanism for a plethora of cancer chemotherapeutics including for example taxenes, anthracyclines, Vinca alkaloids, and epipodopyllotoxins, demonstrated by a large array of published papers, both in tumor cell lines and in a variety of tumors, including various solid tumors and hematological malignancies. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 93-107 29145976-2 2017 Overexpression of a wide-spectrum of ATP-dependent efflux pumps, and in particular of ABCB1 (P-glycoprotein or MDR1) is a well-known resistance mechanism for a plethora of cancer chemotherapeutics including for example taxenes, anthracyclines, Vinca alkaloids, and epipodopyllotoxins, demonstrated by a large array of published papers, both in tumor cell lines and in a variety of tumors, including various solid tumors and hematological malignancies. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 111-115 28283574-1 2017 P-glycoprotein (P-gp) is a multidrug transporter that uses energy from ATP hydrolysis to export many structurally dissimilar hydrophobic and amphipathic compounds, including anticancer drugs from cells. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 28283574-1 2017 P-glycoprotein (P-gp) is a multidrug transporter that uses energy from ATP hydrolysis to export many structurally dissimilar hydrophobic and amphipathic compounds, including anticancer drugs from cells. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 28289287-5 2017 Our findings illuminate how ATP energy is harnessed in the NBDs in a two-stroke cycle and elucidate the consequent conformational motion that reconfigures the TMD, two critical aspects of Pgp transport mechanism. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 188-191 28289287-6 2017 Along with a fully atomistic model of the outward-facing conformation in membranes, the insight into Pgp conformational dynamics harmonizes mechanistic and structural data into a novel perspective on ATP-coupled transport and reveals mechanistic divergence within the efflux class of ABC transporters. Adenosine Triphosphate 200-203 ATP binding cassette subfamily B member 1 Homo sapiens 101-104 26932198-3 2016 P-glycoprotein (P-gp) belongs to the family of adenosine triphosphate (ATP)-binding cassette (ABC) transporters. Adenosine Triphosphate 47-69 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 26932198-3 2016 P-glycoprotein (P-gp) belongs to the family of adenosine triphosphate (ATP)-binding cassette (ABC) transporters. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 26932198-3 2016 P-glycoprotein (P-gp) belongs to the family of adenosine triphosphate (ATP)-binding cassette (ABC) transporters. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 30475507-4 2016 This study focuses on the effect of Maldi 531.2[M+H](+) on P-gp ATPase activity, which was examined by measuring the amount of inorganic phosphates (Pi) released as a result of ATP hydrolysis. Adenosine Triphosphate 64-67 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 27190212-1 2016 The multidrug transporter P-glycoprotein (P-gp, ABCB1) is an ATP-dependent pump that mediates the efflux of structurally diverse drugs and xenobiotics across cell membranes, affecting drug pharmacokinetics and contributing to the development of multidrug resistance. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 26-40 27531061-0 2016 Cooperativity between verapamil and ATP bound to the efflux transporter P-glycoprotein. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 72-86 27531061-1 2016 The P-glycoprotein (Pgp) transporter plays a central role in drug disposition by effluxing a chemically diverse range of drugs from cells through conformational changes and ATP hydrolysis. Adenosine Triphosphate 173-176 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 27531061-1 2016 The P-glycoprotein (Pgp) transporter plays a central role in drug disposition by effluxing a chemically diverse range of drugs from cells through conformational changes and ATP hydrolysis. Adenosine Triphosphate 173-176 ATP binding cassette subfamily B member 1 Homo sapiens 20-23 27531061-2 2016 A number of drugs are known to activate ATP hydrolysis of Pgp, but coupling between ATP and drug binding is not well understood. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 58-61 27531061-2 2016 A number of drugs are known to activate ATP hydrolysis of Pgp, but coupling between ATP and drug binding is not well understood. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 58-61 27531061-4 2016 As previously noted, verapamil-induced Pgp-mediated ATP hydrolysis kinetics was biphasic at saturating ATP concentrations. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 39-42 27531061-4 2016 As previously noted, verapamil-induced Pgp-mediated ATP hydrolysis kinetics was biphasic at saturating ATP concentrations. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 39-42 27531061-6 2016 To further understand this switch in kinetic behavior, the Pgp-coupled ATPase activity kinetics was checked with a panel of verapamil and ATP concentrations and fit with the substrate inhibition equation and the kinetic fitting software COPASI. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 59-62 27531061-8 2016 Fluorescence spectroscopy of Pgp revealed that cooperativity between verapamil and a non-hydrolyzable ATP analog leads to distinct global conformational changes of Pgp. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 29-32 27531061-8 2016 Fluorescence spectroscopy of Pgp revealed that cooperativity between verapamil and a non-hydrolyzable ATP analog leads to distinct global conformational changes of Pgp. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 164-167 27531061-9 2016 NMR of Pgp reconstituted in liposomes showed that cooperativity between verapamil and the non-hydrolyzable ATP analog modulate each other"s interactions. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 7-10 27531061-10 2016 This information was used to produce a conformationally-gated model of drug-induced activation of Pgp-mediated ATP hydrolysis. Adenosine Triphosphate 111-114 ATP binding cassette subfamily B member 1 Homo sapiens 98-101 27785023-5 2016 A series of studies on the action mechanism showed that the polymer components such as beta-cyclodextrin, hydrophobic poly(d,l-lactide) segment, and poly(ethylene glycol) coordinatively contributed to the improved intracellular ATP depletion and ATPase activity, increased intracellular uptake of P-gp substrates via competitive binding to P-gp, and decreased P-gp expression in MCF-7/ADR cells. Adenosine Triphosphate 228-231 ATP binding cassette subfamily B member 1 Homo sapiens 297-301 27785023-5 2016 A series of studies on the action mechanism showed that the polymer components such as beta-cyclodextrin, hydrophobic poly(d,l-lactide) segment, and poly(ethylene glycol) coordinatively contributed to the improved intracellular ATP depletion and ATPase activity, increased intracellular uptake of P-gp substrates via competitive binding to P-gp, and decreased P-gp expression in MCF-7/ADR cells. Adenosine Triphosphate 228-231 ATP binding cassette subfamily B member 1 Homo sapiens 340-344 27785023-5 2016 A series of studies on the action mechanism showed that the polymer components such as beta-cyclodextrin, hydrophobic poly(d,l-lactide) segment, and poly(ethylene glycol) coordinatively contributed to the improved intracellular ATP depletion and ATPase activity, increased intracellular uptake of P-gp substrates via competitive binding to P-gp, and decreased P-gp expression in MCF-7/ADR cells. Adenosine Triphosphate 228-231 ATP binding cassette subfamily B member 1 Homo sapiens 340-344 29599904-1 2018 Ovarian cancers often recur and tumors acquire resistance to chemotherapy due to overexpression of the ATP-dependent efflux pump, multidrug resistance protein 1 (MDR1/P-glycoprotein/ABCB1). Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 130-160 29599904-1 2018 Ovarian cancers often recur and tumors acquire resistance to chemotherapy due to overexpression of the ATP-dependent efflux pump, multidrug resistance protein 1 (MDR1/P-glycoprotein/ABCB1). Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 162-166 29599904-1 2018 Ovarian cancers often recur and tumors acquire resistance to chemotherapy due to overexpression of the ATP-dependent efflux pump, multidrug resistance protein 1 (MDR1/P-glycoprotein/ABCB1). Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 167-187 29599904-3 2018 Instead, in a novel application of our recently described estrogen receptor alpha (ERalpha) biomodulator, BHPI, we targeted MDR1"s substrate, ATP. Adenosine Triphosphate 142-145 ATP binding cassette subfamily B member 1 Homo sapiens 124-128 29599904-11 2018 Targeting MDR1 through UPR-dependent ATP depletion represents a promising therapeutic strategy. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 10-14 27190212-1 2016 The multidrug transporter P-glycoprotein (P-gp, ABCB1) is an ATP-dependent pump that mediates the efflux of structurally diverse drugs and xenobiotics across cell membranes, affecting drug pharmacokinetics and contributing to the development of multidrug resistance. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 42-46 27190212-1 2016 The multidrug transporter P-glycoprotein (P-gp, ABCB1) is an ATP-dependent pump that mediates the efflux of structurally diverse drugs and xenobiotics across cell membranes, affecting drug pharmacokinetics and contributing to the development of multidrug resistance. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 48-53 27190212-2 2016 Structural information about the conformational changes in human P-gp during the ATP hydrolysis cycle has not been directly demonstrated, although mechanistic information has been inferred from biochemical and biophysical studies conducted with P-gp and its orthologs, or from structures of other ATP-binding cassette transporters. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 65-69 27190212-4 2016 We also probe conformational states of human P-gp during the catalytic cycle, and demonstrate that, following ATP hydrolysis, P-gp transitions through a complete closed conformation to a complete open conformation in the presence of ADP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 45-49 27190212-4 2016 We also probe conformational states of human P-gp during the catalytic cycle, and demonstrate that, following ATP hydrolysis, P-gp transitions through a complete closed conformation to a complete open conformation in the presence of ADP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 126-130 26902225-5 2016 We assessed P-gp ATPase activity by monitoring ATP consumption. Adenosine Triphosphate 17-20 ATP binding cassette subfamily B member 1 Homo sapiens 12-16 26733176-8 2016 Above all, P-gp substrate selectively aggravated this ATP depletion effect and increased cell apoptosis in K562/Dox cells. Adenosine Triphosphate 54-57 ATP binding cassette subfamily B member 1 Homo sapiens 11-15 26733176-10 2016 P-gp substrate increases K562/Dox cell apoptosis via aggravating ATP depletion induced by combination of metformin and 2-deoxyglucose. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 27175090-1 2016 The ABCB1 gene encodes a permeability glycoprotein, which is one of the most extensively studied human adenosine-triphosphate (ATP)-dependent efflux transporters. Adenosine Triphosphate 103-125 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 27117502-1 2016 P-glycoprotein (Pgp) is an ABC transporter responsible for the ATP-dependent efflux of chemotherapeutic compounds from multidrug resistant cancer cells. Adenosine Triphosphate 63-66 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 27117502-1 2016 P-glycoprotein (Pgp) is an ABC transporter responsible for the ATP-dependent efflux of chemotherapeutic compounds from multidrug resistant cancer cells. Adenosine Triphosphate 63-66 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 27175090-1 2016 The ABCB1 gene encodes a permeability glycoprotein, which is one of the most extensively studied human adenosine-triphosphate (ATP)-dependent efflux transporters. Adenosine Triphosphate 127-130 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 27364832-1 2016 BACKGROUND: P-glycoprotein (P-gp), a well known ATP dependent efflux membrane transporter, has been attracting considerable interests of medical researchers due to its efflux pump effect being a primary cause of multidrug resistance (MDR) and poor bioavailability (BA) of anticancer agents. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 12-26 26704739-1 2016 P-glycoprotein, encoded by the ABCB1 gene, functions as an ATP-driven efflux pump in the blood-brain barrier, extruding its substrates and thereby limiting their passage into the brain. Adenosine Triphosphate 59-62 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 26704739-1 2016 P-glycoprotein, encoded by the ABCB1 gene, functions as an ATP-driven efflux pump in the blood-brain barrier, extruding its substrates and thereby limiting their passage into the brain. Adenosine Triphosphate 59-62 ATP binding cassette subfamily B member 1 Homo sapiens 31-36 26553253-5 2016 The modulation of P-gp by (R)-7 a involved consumption of ATP, thus demonstrating that the compound behaves as a P-gp substrate. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 18-22 26553253-5 2016 The modulation of P-gp by (R)-7 a involved consumption of ATP, thus demonstrating that the compound behaves as a P-gp substrate. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 113-117 27364832-1 2016 BACKGROUND: P-glycoprotein (P-gp), a well known ATP dependent efflux membrane transporter, has been attracting considerable interests of medical researchers due to its efflux pump effect being a primary cause of multidrug resistance (MDR) and poor bioavailability (BA) of anticancer agents. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 26012726-4 2015 The mechanism of P-gp interaction for each compound was investigated with three biological assays: apparent permeability (Papp ) determination (B A/A B) in Caco-2 cell monolayers, ATP cell depletion, and inhibition of Calcein-AM transport in MDCK-MDR1 cells. Adenosine Triphosphate 180-183 ATP binding cassette subfamily B member 1 Homo sapiens 17-21 26286835-1 2016 MDR1 (multidrug resistance 1) encodes an adenosine triphosphate (ATP)-dependent efflux transporter that plays a fundamental role in transportation of harmful compounds outside cells to maintain optimal health. Adenosine Triphosphate 41-63 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 26286835-1 2016 MDR1 (multidrug resistance 1) encodes an adenosine triphosphate (ATP)-dependent efflux transporter that plays a fundamental role in transportation of harmful compounds outside cells to maintain optimal health. Adenosine Triphosphate 41-63 ATP binding cassette subfamily B member 1 Homo sapiens 6-28 26286835-1 2016 MDR1 (multidrug resistance 1) encodes an adenosine triphosphate (ATP)-dependent efflux transporter that plays a fundamental role in transportation of harmful compounds outside cells to maintain optimal health. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 26286835-1 2016 MDR1 (multidrug resistance 1) encodes an adenosine triphosphate (ATP)-dependent efflux transporter that plays a fundamental role in transportation of harmful compounds outside cells to maintain optimal health. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 6-28 25964294-7 2015 Combining Pgp inhibitors with APO866 led to increased intracellular APO866 levels, compounded NAD(+) and ATP shortage, and induced DeltaPsi(m) dissipation. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 10-13 26381710-1 2015 The ATP binding cassette (ABC) transporters ABCG2 and ABCB1 perform ATP hydrolysis-dependent efflux of structurally highly diverse compounds, collectively called allocrites. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 54-59 25234084-1 2015 The induction of P-glycoprotein (P-gp), an ATP-dependent efflux pump, has been proposed as a strategy against the toxicity induced by P-gp substrates such as the herbicide paraquat (PQ). Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 17-31 25234084-1 2015 The induction of P-glycoprotein (P-gp), an ATP-dependent efflux pump, has been proposed as a strategy against the toxicity induced by P-gp substrates such as the herbicide paraquat (PQ). Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 33-37 25234084-1 2015 The induction of P-glycoprotein (P-gp), an ATP-dependent efflux pump, has been proposed as a strategy against the toxicity induced by P-gp substrates such as the herbicide paraquat (PQ). Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 134-138 26516582-6 2015 In a previous study (Brewer et al., Mol Pharmacol 86: 716-726, 2014), we described how ultrahigh throughput computational searches led to the identification of four drug-like molecules that specifically interfere with the energy harvesting steps of substrate transport and inhibit P-gp catalyzed ATP hydrolysis in vitro. Adenosine Triphosphate 296-299 ATP binding cassette subfamily B member 1 Homo sapiens 281-285 26317651-6 2015 Furthermore, apart from a substrate binding domain, afatinib could also bind to an ATP binding domain of ABCB1 through forming hydrogen bonds with Gly533, Gly534, Lys536 and Ala560 sites. Adenosine Triphosphate 83-86 ATP binding cassette subfamily B member 1 Homo sapiens 105-110 25801244-1 2015 The expression of ATP-dependent efflux pump P-glycoprotein (P-gp) in cancer cells generally results in multidrug resistance (MDR) to chemotherapeutic drugs, which is the main cause of chemotherapy failure in cancer treatment. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 44-58 25801244-1 2015 The expression of ATP-dependent efflux pump P-glycoprotein (P-gp) in cancer cells generally results in multidrug resistance (MDR) to chemotherapeutic drugs, which is the main cause of chemotherapy failure in cancer treatment. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 60-64 25860377-1 2015 ATP-binding cassette transporter B1 (ABCB1; P-glycoprotein; multidrug resistance protein 1) is an adenosine triphosphate (ATP)-dependent efflux transporter located in the plasma membrane of many different cell types. Adenosine Triphosphate 98-120 ATP binding cassette subfamily B member 1 Homo sapiens 0-35 25860377-1 2015 ATP-binding cassette transporter B1 (ABCB1; P-glycoprotein; multidrug resistance protein 1) is an adenosine triphosphate (ATP)-dependent efflux transporter located in the plasma membrane of many different cell types. Adenosine Triphosphate 98-120 ATP binding cassette subfamily B member 1 Homo sapiens 37-42 25860377-1 2015 ATP-binding cassette transporter B1 (ABCB1; P-glycoprotein; multidrug resistance protein 1) is an adenosine triphosphate (ATP)-dependent efflux transporter located in the plasma membrane of many different cell types. Adenosine Triphosphate 98-120 ATP binding cassette subfamily B member 1 Homo sapiens 44-58 25860377-1 2015 ATP-binding cassette transporter B1 (ABCB1; P-glycoprotein; multidrug resistance protein 1) is an adenosine triphosphate (ATP)-dependent efflux transporter located in the plasma membrane of many different cell types. Adenosine Triphosphate 98-120 ATP binding cassette subfamily B member 1 Homo sapiens 60-90 25860377-1 2015 ATP-binding cassette transporter B1 (ABCB1; P-glycoprotein; multidrug resistance protein 1) is an adenosine triphosphate (ATP)-dependent efflux transporter located in the plasma membrane of many different cell types. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 37-42 25860377-1 2015 ATP-binding cassette transporter B1 (ABCB1; P-glycoprotein; multidrug resistance protein 1) is an adenosine triphosphate (ATP)-dependent efflux transporter located in the plasma membrane of many different cell types. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 44-58 25860377-1 2015 ATP-binding cassette transporter B1 (ABCB1; P-glycoprotein; multidrug resistance protein 1) is an adenosine triphosphate (ATP)-dependent efflux transporter located in the plasma membrane of many different cell types. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 60-90 25924616-9 2015 ATP analogs bind MRP1 with reduced apparent affinity, inducing a partially closed conformation. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 17-21 25637762-0 2015 P-glycoprotein interactions of novel psychoactive substances - stimulation of ATP consumption and transport across Caco-2 monolayers. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 25435018-1 2015 P-glycoprotein (P-gp) is an ATP-dependent efflux pump encoded by the MDR1 gene in humans, known to mediate multidrug resistance of neoplastic cells to cancer therapy. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 25435018-1 2015 P-glycoprotein (P-gp) is an ATP-dependent efflux pump encoded by the MDR1 gene in humans, known to mediate multidrug resistance of neoplastic cells to cancer therapy. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 25435018-1 2015 P-glycoprotein (P-gp) is an ATP-dependent efflux pump encoded by the MDR1 gene in humans, known to mediate multidrug resistance of neoplastic cells to cancer therapy. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 69-73 25637762-3 2015 First, the effect on the P-gp ATPase activity was studied by monitoring of ATP consumption after addition to recombinant, human P-gp. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 25-29 25637762-3 2015 First, the effect on the P-gp ATPase activity was studied by monitoring of ATP consumption after addition to recombinant, human P-gp. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 128-132 25685543-3 2015 P-glycoprotein (P-gp) is a member of the ATP-dependent membrane transport proteins; it is known to pump substrates out of cells in ATP-dependent mechanism. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 25962593-1 2015 INTRODUCTION: ATP-binding cassette subfamily B member 1 (ABCB1) and subfamily C member 10 (ABCC10) proteins are efflux transporters that couple the energy derived from ATP hydrolysis to the translocation of toxic substances and chemotherapeutic drugs out of cells. Adenosine Triphosphate 14-17 ATP binding cassette subfamily B member 1 Homo sapiens 57-62 25760620-2 2015 Several structural studies of P-gp and its homologs have provided insights into its transport cycle, but questions remain regarding how P-gp recognizes diverse substrates and how substrate binding is coupled to ATP hydrolysis. Adenosine Triphosphate 211-214 ATP binding cassette subfamily B member 1 Homo sapiens 30-34 26558272-6 2015 The proteins of multidrug gene (the MDR-related protein, MRP1) and P-glycoprotein (P-gp) in MCF-7/MDR cells were downregulated after exposure to 2DG, accompanied with the suppression of the activity of ATP-dependent drug-efflux pump and transmembrane transporter, increasing the intracellular accumulation of DOX to reverse the chemoresistance in multidrug cancer cells. Adenosine Triphosphate 202-205 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 26558272-6 2015 The proteins of multidrug gene (the MDR-related protein, MRP1) and P-glycoprotein (P-gp) in MCF-7/MDR cells were downregulated after exposure to 2DG, accompanied with the suppression of the activity of ATP-dependent drug-efflux pump and transmembrane transporter, increasing the intracellular accumulation of DOX to reverse the chemoresistance in multidrug cancer cells. Adenosine Triphosphate 202-205 ATP binding cassette subfamily B member 1 Homo sapiens 83-87 25450342-7 2015 For P-gp liposomes, the kT value was significantly higher in the presence of ATP than in its absence or in the presence of ATP and the competitive inhibitor verapamil. Adenosine Triphosphate 77-80 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 25450342-7 2015 For P-gp liposomes, the kT value was significantly higher in the presence of ATP than in its absence or in the presence of ATP and the competitive inhibitor verapamil. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 25594233-5 2015 Demethoxycurcumin inhibited P-glycoprotein-mediated ATP hydrolysis under concentrations of <1 muM and efficiently inhibited 200 muM verapamil-stimulated ATPase activity, indicating a high affinity of demethoxycurcumin for P-glycoprotein. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 28-42 25685543-3 2015 P-glycoprotein (P-gp) is a member of the ATP-dependent membrane transport proteins; it is known to pump substrates out of cells in ATP-dependent mechanism. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 25685543-3 2015 P-glycoprotein (P-gp) is a member of the ATP-dependent membrane transport proteins; it is known to pump substrates out of cells in ATP-dependent mechanism. Adenosine Triphosphate 131-134 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 25685543-3 2015 P-glycoprotein (P-gp) is a member of the ATP-dependent membrane transport proteins; it is known to pump substrates out of cells in ATP-dependent mechanism. Adenosine Triphosphate 131-134 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 25869246-4 2015 Consumption of herbal preparations has been reported to alter the function of membrane transporters, especially P-glycoprotein (P-gp), ATP-dependent drug efflux transporter responsible for the development of herb-drug interactions. Adenosine Triphosphate 135-138 ATP binding cassette subfamily B member 1 Homo sapiens 112-126 26235354-1 2015 Overexpression of ATP-dependent efflux pump P-glycoprotein (P-gp) is the main cause of multidrug resistance (MDR) and chemotherapy failure in cancer treatment. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 44-58 26235354-1 2015 Overexpression of ATP-dependent efflux pump P-glycoprotein (P-gp) is the main cause of multidrug resistance (MDR) and chemotherapy failure in cancer treatment. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 60-64 25017650-1 2014 Multidrug resistance (MDR) to chemotherapeutic drugs is the main cause of chemotherapy failure in cancer treatment, and it generally results from expression of ATP-dependent efflux pump P-glycoprotein (P-gp). Adenosine Triphosphate 160-163 ATP binding cassette subfamily B member 1 Homo sapiens 186-200 25201738-6 2014 The measurements of P-gp expression level and mitochondrial membrane potential indicate that the blank HPHM/TPGS2k inhibited P-gp activity by reducing mitochondrial membrane potential and depletion of ATP but without inhibition of P-gp expression. Adenosine Triphosphate 201-204 ATP binding cassette subfamily B member 1 Homo sapiens 125-129 25201738-6 2014 The measurements of P-gp expression level and mitochondrial membrane potential indicate that the blank HPHM/TPGS2k inhibited P-gp activity by reducing mitochondrial membrane potential and depletion of ATP but without inhibition of P-gp expression. Adenosine Triphosphate 201-204 ATP binding cassette subfamily B member 1 Homo sapiens 125-129 25016028-0 2014 The Q loops of the human multidrug resistance transporter ABCB1 are necessary to couple drug binding to the ATP catalytic cycle. Adenosine Triphosphate 108-111 ATP binding cassette subfamily B member 1 Homo sapiens 58-63 25016028-1 2014 For a primary active pump, such as the human ATP-binding-cassette (ABC) transporter ABCB1, coupling of drug-binding by the two transmembrane domains (TMDs) to the ATP catalytic cycle of the two nucleotide-binding domains (NBDs) is fundamental to the transport mechanism, but is poorly understood at the biochemical level. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 84-89 25016028-1 2014 For a primary active pump, such as the human ATP-binding-cassette (ABC) transporter ABCB1, coupling of drug-binding by the two transmembrane domains (TMDs) to the ATP catalytic cycle of the two nucleotide-binding domains (NBDs) is fundamental to the transport mechanism, but is poorly understood at the biochemical level. Adenosine Triphosphate 163-166 ATP binding cassette subfamily B member 1 Homo sapiens 84-89 25270578-8 2014 Four compounds were found that inhibit ATP hydrolysis by P-gp. Adenosine Triphosphate 39-42 ATP binding cassette subfamily B member 1 Homo sapiens 57-61 25270578-3 2014 P-gp uses ATP hydrolysis to catalyze the transport of a broad range of mostly hydrophobic compounds across the plasma membrane and out of the cell. Adenosine Triphosphate 10-13 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 25270578-5 2014 The present study describes extremely high-throughput, massively parallel in silico ligand docking studies aimed at identifying reversible inhibitors of ATP hydrolysis that target the nucleotide-binding domains of P-gp. Adenosine Triphosphate 153-156 ATP binding cassette subfamily B member 1 Homo sapiens 214-218 25281745-2 2014 However, it is now clear that MRP1 serves a broader role than simply mediating the ATP-dependent efflux of drugs from cells. Adenosine Triphosphate 83-86 ATP binding cassette subfamily B member 1 Homo sapiens 30-34 25372840-9 2014 The intracellular level of ATP decreased 44%, 46% in the presence of 12.5.25 microM 3-Bromopyruvate, whereas the accumulation of rhodamine 123 and epirubicin (two typical P-glycoprotein substrates) in cells was significantly increased. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 171-185 25017650-1 2014 Multidrug resistance (MDR) to chemotherapeutic drugs is the main cause of chemotherapy failure in cancer treatment, and it generally results from expression of ATP-dependent efflux pump P-glycoprotein (P-gp). Adenosine Triphosphate 160-163 ATP binding cassette subfamily B member 1 Homo sapiens 202-206 24643703-2 2014 One of the main underlying mechanisms of this resistance is the over-expression of P-glycoprotein (P-gp), an ATP-dependent transmembrane transporter protein encoded by the MDR1 gene. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 83-97 24643703-2 2014 One of the main underlying mechanisms of this resistance is the over-expression of P-glycoprotein (P-gp), an ATP-dependent transmembrane transporter protein encoded by the MDR1 gene. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 99-103 24643703-2 2014 One of the main underlying mechanisms of this resistance is the over-expression of P-glycoprotein (P-gp), an ATP-dependent transmembrane transporter protein encoded by the MDR1 gene. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 172-176 24897122-6 2014 P-Glycoprotein has been proposed to hydrolyse ATP by an alternating mechanism, for which there is substantial experimental evidence, including inhibition of catalytic activity by trapping of ortho-vanadate at one nucleotide-binding domain, and the observation of an asymmetric occluded state. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 24897122-8 2014 In this work, we have built several progressively more complex kinetic models, and then carried out simulations and detailed analysis, to test the validity of the proposed reaction pathway employed by P-glycoprotein for ATP hydrolysis. Adenosine Triphosphate 220-223 ATP binding cassette subfamily B member 1 Homo sapiens 201-215 24897122-9 2014 To establish kinetic parameters for the catalytic cycle, we made use of the large amount of published data on ATP hydrolysis by hamster P-glycoprotein, both purified and in membrane vesicles. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 136-150 24613626-1 2014 P-glycoprotein (P-gp) is an ATP-dependent multidrug resistance efflux transporter that plays an important role in anticancer drug resistance and in pharmacokinetics of medicines. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 24773054-4 2014 Compound 28 inhibited the photolabeling of P-gp with [(125)I]-iodoarylazidoprazosin with IC50 = 0.75 muM and stimulated the basal ATP hydrolysis of P-gp in a concentration-dependent manner (EC50 ATPase = 0.027 muM). Adenosine Triphosphate 130-133 ATP binding cassette subfamily B member 1 Homo sapiens 148-152 24613626-1 2014 P-glycoprotein (P-gp) is an ATP-dependent multidrug resistance efflux transporter that plays an important role in anticancer drug resistance and in pharmacokinetics of medicines. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 24107928-1 2014 The efflux transporters adenosine triphosphate (ATP)-binding cassette (ABC)B1 and ABCG2 have been demonstrated to interact with the tyrosine kinase inhibitors (TKIs) imatinib, nilotinib, and dasatinib. Adenosine Triphosphate 24-46 ATP binding cassette subfamily B member 1 Homo sapiens 71-77 24711707-1 2014 P-glycoprotein (P-gp) is an ATP (adenosine triphosphate)-binding cassette transporter that causes multidrug resistance of various chemotherapeutic substances by active efflux from mammalian cells. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 24711707-1 2014 P-glycoprotein (P-gp) is an ATP (adenosine triphosphate)-binding cassette transporter that causes multidrug resistance of various chemotherapeutic substances by active efflux from mammalian cells. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 24711707-1 2014 P-glycoprotein (P-gp) is an ATP (adenosine triphosphate)-binding cassette transporter that causes multidrug resistance of various chemotherapeutic substances by active efflux from mammalian cells. Adenosine Triphosphate 33-55 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 24711707-1 2014 P-glycoprotein (P-gp) is an ATP (adenosine triphosphate)-binding cassette transporter that causes multidrug resistance of various chemotherapeutic substances by active efflux from mammalian cells. Adenosine Triphosphate 33-55 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 24632881-0 2014 Structural characterization of two metastable ATP-bound states of P-glycoprotein. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 66-80 24632881-3 2014 Here, changes in the conformation of the ABC export protein P-glycoprotein on ATP binding are examined in a series of molecular dynamics simulations. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 24632881-4 2014 When one molecule of ATP is placed at the ATP binding site associated with each of the two nucleotide binding domains (NBDs), the membrane-embedded P-glycoprotein crystal structure adopts two distinct metastable conformations. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 148-162 24632881-4 2014 When one molecule of ATP is placed at the ATP binding site associated with each of the two nucleotide binding domains (NBDs), the membrane-embedded P-glycoprotein crystal structure adopts two distinct metastable conformations. Adenosine Triphosphate 42-45 ATP binding cassette subfamily B member 1 Homo sapiens 148-162 24231430-1 2014 Multidrug resistance protein 1 (MRP1) extrudes drugs as well as pharmacologically and physiologically important organic anions across the plasma membrane in an ATP-dependent manner. Adenosine Triphosphate 160-163 ATP binding cassette subfamily B member 1 Homo sapiens 0-30 24231430-1 2014 Multidrug resistance protein 1 (MRP1) extrudes drugs as well as pharmacologically and physiologically important organic anions across the plasma membrane in an ATP-dependent manner. Adenosine Triphosphate 160-163 ATP binding cassette subfamily B member 1 Homo sapiens 32-36 24107928-1 2014 The efflux transporters adenosine triphosphate (ATP)-binding cassette (ABC)B1 and ABCG2 have been demonstrated to interact with the tyrosine kinase inhibitors (TKIs) imatinib, nilotinib, and dasatinib. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 71-77 24329094-1 2013 P-glycoprotein (P-gp), a kind of ATP-binding cassette transporter, can export candidates through a channel at the two transmembrane domains (TMDs) across the cell membranes using the energy released from ATP hydrolysis at the two nucleotide-binding domains (NBDs). Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 25169536-3 2014 The AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. Adenosine Triphosphate 203-206 ATP binding cassette subfamily B member 1 Homo sapiens 234-248 25169536-3 2014 The AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. Adenosine Triphosphate 203-206 ATP binding cassette subfamily B member 1 Homo sapiens 250-253 25169536-3 2014 The AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. Adenosine Triphosphate 203-206 ATP binding cassette subfamily B member 1 Homo sapiens 279-284 24266329-1 2013 The expression of P-glycoprotein (P-gp), an ATP-dependent efflux transporter, is closely associated with the failure of chemotherapy and drug absorption. Adenosine Triphosphate 44-47 ATP binding cassette subfamily B member 1 Homo sapiens 18-32 24266329-1 2013 The expression of P-glycoprotein (P-gp), an ATP-dependent efflux transporter, is closely associated with the failure of chemotherapy and drug absorption. Adenosine Triphosphate 44-47 ATP binding cassette subfamily B member 1 Homo sapiens 34-38 24506763-0 2014 Reaction dynamics of ATP hydrolysis catalyzed by P-glycoprotein. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 49-63 24506763-2 2014 P-gp couples drug efflux with ATP hydrolysis by coordinating conformational changes in the drug binding sites with the hydrolysis of ATP and release of ADP. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 24506763-2 2014 P-gp couples drug efflux with ATP hydrolysis by coordinating conformational changes in the drug binding sites with the hydrolysis of ATP and release of ADP. Adenosine Triphosphate 133-136 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 24256068-7 2014 The depletion of intracellular ATP, which was likely to be a result of partial inhibition of cellular metabolism, was directly correlated with inhibitory potential for P-gp mediated efflux by mPEG-PLA analogues. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 168-172 24256068-8 2014 Hence, intracellular ATP-depletion appeared to be possible explanation to the inhibition mechanism of P-gp by mPEG-PLA. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 102-106 24329094-1 2013 P-glycoprotein (P-gp), a kind of ATP-binding cassette transporter, can export candidates through a channel at the two transmembrane domains (TMDs) across the cell membranes using the energy released from ATP hydrolysis at the two nucleotide-binding domains (NBDs). Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 24349290-2 2013 Pgp transports a variety of chemically dissimilar amphipathic compounds using the energy from ATP hydrolysis. Adenosine Triphosphate 94-97 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 24277991-2 2013 The limitation of berberine seems to be its poor oral bioavailability, which is affected by the presence, in enterocytes, of P-glycoprotein - an active adenosine triphosphate (ATP)-consuming efflux protein that extrudes berberine into the intestinal lumen, thus limiting its absorption. Adenosine Triphosphate 152-174 ATP binding cassette subfamily B member 1 Homo sapiens 125-139 24277991-2 2013 The limitation of berberine seems to be its poor oral bioavailability, which is affected by the presence, in enterocytes, of P-glycoprotein - an active adenosine triphosphate (ATP)-consuming efflux protein that extrudes berberine into the intestinal lumen, thus limiting its absorption. Adenosine Triphosphate 176-179 ATP binding cassette subfamily B member 1 Homo sapiens 125-139 24064216-2 2013 Computational models of human P-gp in the apo- and nucleotide-bound conformation show that the adenine group of ATP forms hydrogen bonds with the conserved Asp-164 and Asp-805 in intracellular loops 1 and 3, respectively, which are located at the interface between the nucleotide binding domains and transmembrane domains. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 30-34 23917219-2 2013 MDR has been primarily attributed by the ATP-dependent transmembrane proteins, P-glycoprotein (P-gp, ABCB1) and Multidrug Resistance-Associated Protein 1 (MRP1, ABCC1). Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 79-93 22641028-1 2013 P-glycoprotein (P-gp), an ATP-driven efflux pump in the blood-brain barrier, has a major impact on the delivery of antidepressant drugs in the brain. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 22641028-1 2013 P-glycoprotein (P-gp), an ATP-driven efflux pump in the blood-brain barrier, has a major impact on the delivery of antidepressant drugs in the brain. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 23520057-1 2013 BACKGROUND: P-glycoprotein (P-gp) is an adenosine triphosphate (ATP)-dependent efflux pump that confers chemotherapeutic resistance in cancer cells. Adenosine Triphosphate 40-62 ATP binding cassette subfamily B member 1 Homo sapiens 12-26 23520057-1 2013 BACKGROUND: P-glycoprotein (P-gp) is an adenosine triphosphate (ATP)-dependent efflux pump that confers chemotherapeutic resistance in cancer cells. Adenosine Triphosphate 40-62 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 23520057-1 2013 BACKGROUND: P-glycoprotein (P-gp) is an adenosine triphosphate (ATP)-dependent efflux pump that confers chemotherapeutic resistance in cancer cells. Adenosine Triphosphate 64-67 ATP binding cassette subfamily B member 1 Homo sapiens 12-26 23520057-1 2013 BACKGROUND: P-glycoprotein (P-gp) is an adenosine triphosphate (ATP)-dependent efflux pump that confers chemotherapeutic resistance in cancer cells. Adenosine Triphosphate 64-67 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 23687985-1 2013 BACKGROUND: The multidrug resistance (MDR) 1 gene encodes a 170-kDa membrane transporter called P-glycoprotein, which plays an important role in protecting cells against lipophilic xenobiotics by the way of an ATP-dependent cellular efflux mechanism. Adenosine Triphosphate 210-213 ATP binding cassette subfamily B member 1 Homo sapiens 16-44 23088763-1 2013 MDR1 encodes an adenosine triphosphate (ATP)-dependent efflux transporter that protects the body from environmental xenobiotics to maintain optimal health. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 24377601-3 2013 Multiple drug resistance 1/P-glycoprotein (MDR1/p-gp) contributes to drug resistance via ATP-dependent drug efflux pumps and is overexpressed in many solid tumors including gastric cancer. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 27-41 23478574-1 2013 The multidrug resistance 1 (MDR1) gene product P-glycoprotein is an ATP-dependent efflux pump associated with chemotherapy failure in breast cancer. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 4-26 23478574-1 2013 The multidrug resistance 1 (MDR1) gene product P-glycoprotein is an ATP-dependent efflux pump associated with chemotherapy failure in breast cancer. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 23478574-1 2013 The multidrug resistance 1 (MDR1) gene product P-glycoprotein is an ATP-dependent efflux pump associated with chemotherapy failure in breast cancer. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 47-61 23089310-7 2013 Intracellular ATP depletion was the main mechanism of Pluronic P123/F127 for P-gp inhibition. Adenosine Triphosphate 14-17 ATP binding cassette subfamily B member 1 Homo sapiens 77-81 23617068-1 2013 P-glycoprotein (P-gp), an ATP-dependant efflux pump transports a wide range of substrates across cellular membranes. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 23617068-1 2013 P-glycoprotein (P-gp), an ATP-dependant efflux pump transports a wide range of substrates across cellular membranes. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 23646646-3 2013 Thus, P-gp inhibitors have attracted a lot of attention as they can stop cancer drugs from being pumped out of target cells with the consumption of ATP. Adenosine Triphosphate 148-151 ATP binding cassette subfamily B member 1 Homo sapiens 6-10 23104431-3 2013 P-glycoprotein (P-gp; ABCB1) is the only known ATP-dependent efflux transporter that handles digoxin as a substrate. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 23104431-3 2013 P-glycoprotein (P-gp; ABCB1) is the only known ATP-dependent efflux transporter that handles digoxin as a substrate. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 23104431-3 2013 P-glycoprotein (P-gp; ABCB1) is the only known ATP-dependent efflux transporter that handles digoxin as a substrate. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 22-27 24377601-3 2013 Multiple drug resistance 1/P-glycoprotein (MDR1/p-gp) contributes to drug resistance via ATP-dependent drug efflux pumps and is overexpressed in many solid tumors including gastric cancer. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 43-47 22420656-1 2012 OBJECTIVES: Opioids and anticancer compounds such as etoposide (ETP) are substrates of P-glycoprotein (P-gp), an ATP-dependent efflux pump. Adenosine Triphosphate 113-116 ATP binding cassette subfamily B member 1 Homo sapiens 87-101 23149859-10 2012 After SN-38 was released from TPGS/PLGA/SN-38 NPs in MDR cells, TPGS or/and PLGA may modulate the efflux microenvironment of the P-gp pump, such as mitochondria and the P-gp domain with an ATP-binding site. Adenosine Triphosphate 189-192 ATP binding cassette subfamily B member 1 Homo sapiens 129-133 22572929-2 2012 Multidrug resistance (MDR) is often characterized by the expression of P-glycoprotein (P-gp), a 170-KD ATP-dependent drug efflux protein. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 71-85 22572929-2 2012 Multidrug resistance (MDR) is often characterized by the expression of P-glycoprotein (P-gp), a 170-KD ATP-dependent drug efflux protein. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 23457608-7 2013 In contrast, activity of the MDR1 ABC transporter was reduced by air exposure and the drugs that inhibited air-stimulated ATP release had differential effects on this transporter. Adenosine Triphosphate 122-125 ATP binding cassette subfamily B member 1 Homo sapiens 29-33 22832898-4 2012 The multidrug resistance phenotype was initially associated to P-glycoprotein (Pgp), an ATP-dependent transporter belonging to the same superfamily of multidrug resistance-associated proteins (MRPs). Adenosine Triphosphate 88-91 ATP binding cassette subfamily B member 1 Homo sapiens 63-77 22832898-4 2012 The multidrug resistance phenotype was initially associated to P-glycoprotein (Pgp), an ATP-dependent transporter belonging to the same superfamily of multidrug resistance-associated proteins (MRPs). Adenosine Triphosphate 88-91 ATP binding cassette subfamily B member 1 Homo sapiens 79-82 22311042-1 2012 Multidrug resistance 1 (MDR1) gene encodes the ATP-dependent cellular efflux pump P-glycoprotein (P-gp) which efflux of a variety of substances across the membrane. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 0-22 22311042-1 2012 Multidrug resistance 1 (MDR1) gene encodes the ATP-dependent cellular efflux pump P-glycoprotein (P-gp) which efflux of a variety of substances across the membrane. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 24-28 22311042-1 2012 Multidrug resistance 1 (MDR1) gene encodes the ATP-dependent cellular efflux pump P-glycoprotein (P-gp) which efflux of a variety of substances across the membrane. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 82-96 22311042-1 2012 Multidrug resistance 1 (MDR1) gene encodes the ATP-dependent cellular efflux pump P-glycoprotein (P-gp) which efflux of a variety of substances across the membrane. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 98-102 22647192-1 2012 Multidrug resistance proteins that belong to the ATP-binding cassette family like the human P-glycoprotein (ABCB1 or Pgp) are responsible for many failed cancer and antiviral chemotherapies because these membrane transporters remove the chemotherapeutics from the targeted cells. Adenosine Triphosphate 49-52 ATP binding cassette subfamily B member 1 Homo sapiens 108-113 22764569-8 2012 In addition, their affinity and binding rate to MDR1 was examined by ATP activity assay. Adenosine Triphosphate 69-72 ATP binding cassette subfamily B member 1 Homo sapiens 48-52 22420656-1 2012 OBJECTIVES: Opioids and anticancer compounds such as etoposide (ETP) are substrates of P-glycoprotein (P-gp), an ATP-dependent efflux pump. Adenosine Triphosphate 113-116 ATP binding cassette subfamily B member 1 Homo sapiens 103-107 21967057-9 2011 The nucleotide-binding domains of Pgp appear to hydrolyse ATP in an alternating manner; however, it is still not clear whether transport is driven by ATP hydrolysis or ATP binding. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 34-37 20641391-1 2004 P-gp is an ATP-dependent transmembrane multidrug transporter that is capable of actively pumping a variety of agents out of cells. Adenosine Triphosphate 11-14 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 20641916-1 2004 P-gp is an ATP-dependent transmembrane multidrug transporter that is capable of actively pumping a variety of agents out of cells. Adenosine Triphosphate 11-14 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 22086917-1 2012 P-glycoprotein (Pgp), a member of the ATP-binding cassette transporter family, functions as an ATP hydrolysis-driven efflux pump to rid the cell of toxic organic compounds, including a variety of drugs used in anticancer chemotherapy. Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 22086917-1 2012 P-glycoprotein (Pgp), a member of the ATP-binding cassette transporter family, functions as an ATP hydrolysis-driven efflux pump to rid the cell of toxic organic compounds, including a variety of drugs used in anticancer chemotherapy. Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 22044878-7 2012 The best P-gp inhibitor found was 1-[2-(1H-benzimidazol-2-yl)ethanamine]-4-propoxy-9H-thioxanthen-9-one (45), which caused an accumulation rate of rhodamine-123 similar to that caused by verapamil in the K562Dox resistant cell line, and a decrease in ATP consumption by P-gp. Adenosine Triphosphate 251-254 ATP binding cassette subfamily B member 1 Homo sapiens 9-13 21937435-1 2011 The human ATP-binding cassette (ABC) transporter, P-glycoprotein (P-gp; ABCB1), mediates the ATP-dependent efflux of a variety of drugs. Adenosine Triphosphate 10-13 ATP binding cassette subfamily B member 1 Homo sapiens 50-64 21937435-1 2011 The human ATP-binding cassette (ABC) transporter, P-glycoprotein (P-gp; ABCB1), mediates the ATP-dependent efflux of a variety of drugs. Adenosine Triphosphate 10-13 ATP binding cassette subfamily B member 1 Homo sapiens 66-70 21937435-1 2011 The human ATP-binding cassette (ABC) transporter, P-glycoprotein (P-gp; ABCB1), mediates the ATP-dependent efflux of a variety of drugs. Adenosine Triphosphate 10-13 ATP binding cassette subfamily B member 1 Homo sapiens 72-77 22275834-1 2012 BACKGROUND: Multidrug resistance (MDR) mediated by the overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) transporters, such as P-glycoprotein (P-gp), remains one of the major obstacles to effective cancer chemotherapy. Adenosine Triphosphate 73-95 ATP binding cassette subfamily B member 1 Homo sapiens 147-161 22275834-1 2012 BACKGROUND: Multidrug resistance (MDR) mediated by the overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) transporters, such as P-glycoprotein (P-gp), remains one of the major obstacles to effective cancer chemotherapy. Adenosine Triphosphate 97-100 ATP binding cassette subfamily B member 1 Homo sapiens 147-161 22275834-1 2012 BACKGROUND: Multidrug resistance (MDR) mediated by the overexpression of adenosine triphosphate (ATP)-binding cassette (ABC) transporters, such as P-glycoprotein (P-gp), remains one of the major obstacles to effective cancer chemotherapy. Adenosine Triphosphate 97-100 ATP binding cassette subfamily B member 1 Homo sapiens 163-167 21625253-1 2011 The human ABCB1 protein, (P-glycoprotein or MDR1) is a membrane-bound glycoprotein that harnesses the energy of ATP hydrolysis to drive the unidirectional transport of substrates from the cytoplasm to the extracellular space. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 10-15 21625253-1 2011 The human ABCB1 protein, (P-glycoprotein or MDR1) is a membrane-bound glycoprotein that harnesses the energy of ATP hydrolysis to drive the unidirectional transport of substrates from the cytoplasm to the extracellular space. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 26-40 21625253-1 2011 The human ABCB1 protein, (P-glycoprotein or MDR1) is a membrane-bound glycoprotein that harnesses the energy of ATP hydrolysis to drive the unidirectional transport of substrates from the cytoplasm to the extracellular space. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 44-48 21967057-1 2011 Pgp (P-glycoprotein) (ABCB1) is an ATP-powered efflux pump which can transport hundreds of structurally unrelated hydrophobic amphipathic compounds, including therapeutic drugs, peptides and lipid-like compounds. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 21967057-1 2011 Pgp (P-glycoprotein) (ABCB1) is an ATP-powered efflux pump which can transport hundreds of structurally unrelated hydrophobic amphipathic compounds, including therapeutic drugs, peptides and lipid-like compounds. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 5-19 21967057-1 2011 Pgp (P-glycoprotein) (ABCB1) is an ATP-powered efflux pump which can transport hundreds of structurally unrelated hydrophobic amphipathic compounds, including therapeutic drugs, peptides and lipid-like compounds. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 22-27 21967057-9 2011 The nucleotide-binding domains of Pgp appear to hydrolyse ATP in an alternating manner; however, it is still not clear whether transport is driven by ATP hydrolysis or ATP binding. Adenosine Triphosphate 150-153 ATP binding cassette subfamily B member 1 Homo sapiens 34-37 21967057-9 2011 The nucleotide-binding domains of Pgp appear to hydrolyse ATP in an alternating manner; however, it is still not clear whether transport is driven by ATP hydrolysis or ATP binding. Adenosine Triphosphate 150-153 ATP binding cassette subfamily B member 1 Homo sapiens 34-37 21246559-2 2011 P-glycoprotein (P-gp) contributes to drug resistance via adenosine 5"-triphosphate (ATP)-dependent drug efflux pumps and is widely expressed in many human cancers. Adenosine Triphosphate 57-82 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 22829191-5 2011 Upregulation of EGF and JAG1 oncogenes as well as genes encoding ATP-dependent drug efflux pump proteins such as ABCB1 was also observed in the resistant cells, which may confer alternative survival benefits. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 113-118 21246559-2 2011 P-glycoprotein (P-gp) contributes to drug resistance via adenosine 5"-triphosphate (ATP)-dependent drug efflux pumps and is widely expressed in many human cancers. Adenosine Triphosphate 57-82 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21246559-2 2011 P-glycoprotein (P-gp) contributes to drug resistance via adenosine 5"-triphosphate (ATP)-dependent drug efflux pumps and is widely expressed in many human cancers. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21246559-2 2011 P-glycoprotein (P-gp) contributes to drug resistance via adenosine 5"-triphosphate (ATP)-dependent drug efflux pumps and is widely expressed in many human cancers. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21452853-11 2011 Thus, FSBA is an ATP analogue that interacts with both the drug-binding and ATP-binding sites of P-gp, but fluorosulfonyl-mediated cross-linking is observed only at the NBDs. Adenosine Triphosphate 17-20 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 21452853-11 2011 Thus, FSBA is an ATP analogue that interacts with both the drug-binding and ATP-binding sites of P-gp, but fluorosulfonyl-mediated cross-linking is observed only at the NBDs. Adenosine Triphosphate 76-79 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 21039338-1 2011 ATP-binding cassette (ABC) transporters, P-glycoprotein (P-gp, ABCB1) and ABCG2, are membrane proteins that couple the energy derived from ATP hydrolysis to efflux many chemically diverse compounds across the plasma membrane, thereby playing a critical and important physiological role in protecting cells from xenobiotics. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 41-55 21452853-0 2011 Inhibition of multidrug resistance-linked P-glycoprotein (ABCB1) function by 5"-fluorosulfonylbenzoyl 5"-adenosine: evidence for an ATP analogue that interacts with both drug-substrate-and nucleotide-binding sites. Adenosine Triphosphate 132-135 ATP binding cassette subfamily B member 1 Homo sapiens 42-56 21452853-0 2011 Inhibition of multidrug resistance-linked P-glycoprotein (ABCB1) function by 5"-fluorosulfonylbenzoyl 5"-adenosine: evidence for an ATP analogue that interacts with both drug-substrate-and nucleotide-binding sites. Adenosine Triphosphate 132-135 ATP binding cassette subfamily B member 1 Homo sapiens 58-63 21452853-2 2011 P-glycoprotein (P-gp, ABCB1) is a member of the ATP-binding cassette (ABC) transporter superfamily that utilizes energy from ATP hydrolysis for the efflux of amphipathic anticancer agents from cancer cells. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21452853-2 2011 P-glycoprotein (P-gp, ABCB1) is a member of the ATP-binding cassette (ABC) transporter superfamily that utilizes energy from ATP hydrolysis for the efflux of amphipathic anticancer agents from cancer cells. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21452853-2 2011 P-glycoprotein (P-gp, ABCB1) is a member of the ATP-binding cassette (ABC) transporter superfamily that utilizes energy from ATP hydrolysis for the efflux of amphipathic anticancer agents from cancer cells. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 22-27 21452853-3 2011 We investigated the interactions of FSBA with P-gp to study the catalytic cycle of ATP hydrolysis. Adenosine Triphosphate 83-86 ATP binding cassette subfamily B member 1 Homo sapiens 46-50 21452853-4 2011 Incubation of P-gp with FSBA inhibited ATP hydrolysis (IC(50 )= 0.21 mM) and the binding of 8-azido[alpha-(32)P]ATP (IC(50) = 0.68 mM). Adenosine Triphosphate 39-42 ATP binding cassette subfamily B member 1 Homo sapiens 14-18 21452853-5 2011 In addition, (14)C-FSBA cross-links to P-gp, suggesting that FSBA-mediated inhibition of ATP hydrolysis is irreversible due to covalent modification of P-gp. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 39-43 21452853-5 2011 In addition, (14)C-FSBA cross-links to P-gp, suggesting that FSBA-mediated inhibition of ATP hydrolysis is irreversible due to covalent modification of P-gp. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 152-156 21452853-6 2011 However, when the NBDs were occupied with a saturating concentration of ATP prior to treatment, FSBA stimulated ATP hydrolysis by P-gp. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 130-134 21452853-6 2011 However, when the NBDs were occupied with a saturating concentration of ATP prior to treatment, FSBA stimulated ATP hydrolysis by P-gp. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 130-134 21039338-1 2011 ATP-binding cassette (ABC) transporters, P-glycoprotein (P-gp, ABCB1) and ABCG2, are membrane proteins that couple the energy derived from ATP hydrolysis to efflux many chemically diverse compounds across the plasma membrane, thereby playing a critical and important physiological role in protecting cells from xenobiotics. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 57-61 21039338-1 2011 ATP-binding cassette (ABC) transporters, P-glycoprotein (P-gp, ABCB1) and ABCG2, are membrane proteins that couple the energy derived from ATP hydrolysis to efflux many chemically diverse compounds across the plasma membrane, thereby playing a critical and important physiological role in protecting cells from xenobiotics. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 63-68 21247706-5 2011 P-glycoprotein (P-gp), member of ABC transporter family, has been described for drug resistance and a low bioavailability of drugs by pumping structurally unrelated drugs out of the cells at the cost of ATP hydrolysis. Adenosine Triphosphate 203-206 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21411714-2 2011 Overexpression of ATP-dependent efflux transporter P-glycoprotein (PGP) is a key factor contributing to multidrug resistance of cancer cells. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 51-65 21411714-2 2011 Overexpression of ATP-dependent efflux transporter P-glycoprotein (PGP) is a key factor contributing to multidrug resistance of cancer cells. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 67-70 21308736-5 2011 Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 63-68 21247706-5 2011 P-glycoprotein (P-gp), member of ABC transporter family, has been described for drug resistance and a low bioavailability of drugs by pumping structurally unrelated drugs out of the cells at the cost of ATP hydrolysis. Adenosine Triphosphate 203-206 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21348299-1 2010 P-glycoprotein (P-gp) is an ATP-dependent multidrug efflux pump that acts as a major obstacle for oral drug delivery and cancer therapy. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21278250-1 2011 P-glycoprotein (Pgp), a member of the ABC transporter family, functions as an ATP hydrolysis-driven efflux pump to rid the cell of toxic organic compounds, including a variety of drugs used in anti-cancer chemotherapy. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21278250-1 2011 P-glycoprotein (Pgp), a member of the ABC transporter family, functions as an ATP hydrolysis-driven efflux pump to rid the cell of toxic organic compounds, including a variety of drugs used in anti-cancer chemotherapy. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 20731718-10 2010 Taken together, these results indicate that TM12 plays a key role in the progression of the ATP hydrolytic cycle in ABCB1 and, in particular, in coordinating conformational changes between the NBDs and transmembrane domains. Adenosine Triphosphate 92-95 ATP binding cassette subfamily B member 1 Homo sapiens 116-121 20862795-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 20862795-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 39-53 20862795-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 55-59 21448449-5 2011 Cabazitaxel is a novel tubulin-binding taxane that differs from docetaxel because of its poor affinity for P-glycoprotein (P-gp), an ATP-dependent drug efflux pump. Adenosine Triphosphate 133-136 ATP binding cassette subfamily B member 1 Homo sapiens 107-121 21448449-5 2011 Cabazitaxel is a novel tubulin-binding taxane that differs from docetaxel because of its poor affinity for P-glycoprotein (P-gp), an ATP-dependent drug efflux pump. Adenosine Triphosphate 133-136 ATP binding cassette subfamily B member 1 Homo sapiens 123-127 21269449-4 2011 P-glycoprotein (P-gp) is most commonly associated with human MDR; it utilizes energy from adenosine triphosphate (ATP) to transport a number of substrates out of cells against concentration gradients. Adenosine Triphosphate 90-112 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21269449-4 2011 P-glycoprotein (P-gp) is most commonly associated with human MDR; it utilizes energy from adenosine triphosphate (ATP) to transport a number of substrates out of cells against concentration gradients. Adenosine Triphosphate 90-112 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21269449-4 2011 P-glycoprotein (P-gp) is most commonly associated with human MDR; it utilizes energy from adenosine triphosphate (ATP) to transport a number of substrates out of cells against concentration gradients. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21269449-4 2011 P-glycoprotein (P-gp) is most commonly associated with human MDR; it utilizes energy from adenosine triphosphate (ATP) to transport a number of substrates out of cells against concentration gradients. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21695098-4 2011 In the last year, cabazitaxel, a novel taxane with decreased affinity for ATP-dependent drug efflux pump P-glycoprotein, became the first cytotoxic agent to demonstrate an improvement in survival in men with docetaxel-refractory disease, and has received regulatory approval for treatment in this setting. Adenosine Triphosphate 74-77 ATP binding cassette subfamily B member 1 Homo sapiens 105-119 20862794-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 20862794-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 39-53 20862794-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 55-59 21348299-1 2010 P-glycoprotein (P-gp) is an ATP-dependent multidrug efflux pump that acts as a major obstacle for oral drug delivery and cancer therapy. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21348299-4 2010 Other than competitive inhibition for traditional inhibitors, polymeric inhibitors may modify P-gp function through alterations on membrane fluidity, inhibition of P-gp ATPase, depletion of intracellular ATP and down-regulating of P-gp expression. Adenosine Triphosphate 169-172 ATP binding cassette subfamily B member 1 Homo sapiens 94-98 21348299-4 2010 Other than competitive inhibition for traditional inhibitors, polymeric inhibitors may modify P-gp function through alterations on membrane fluidity, inhibition of P-gp ATPase, depletion of intracellular ATP and down-regulating of P-gp expression. Adenosine Triphosphate 169-172 ATP binding cassette subfamily B member 1 Homo sapiens 164-168 21348299-4 2010 Other than competitive inhibition for traditional inhibitors, polymeric inhibitors may modify P-gp function through alterations on membrane fluidity, inhibition of P-gp ATPase, depletion of intracellular ATP and down-regulating of P-gp expression. Adenosine Triphosphate 169-172 ATP binding cassette subfamily B member 1 Homo sapiens 164-168 20137798-1 2010 Multidrug resistance 1 (MDR1) and multidrug resistance-associated protein 2 (MRP2) are two prominent members of the super-family of ATP-binding cassette (ABC) transporters that carry a wide range of substrates across biological membranes, using ATP as energy source. Adenosine Triphosphate 132-135 ATP binding cassette subfamily B member 1 Homo sapiens 24-28 20061384-1 2010 P-glycoprotein (ABCB1), a member of the ABC superfamily, functions as an ATP-driven multidrug efflux pump. Adenosine Triphosphate 73-76 ATP binding cassette subfamily B member 1 Homo sapiens 16-21 20662321-1 2010 P-Glycoprotein, a 170-180 kDa membrane glycoprotein that mediates multidrug resistance, hydrolyses ATP to efflux a broad spectrum of hydrophobic agents. Adenosine Triphosphate 99-102 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 20205474-0 2010 Vitamin E TPGS P-glycoprotein inhibition mechanism: influence on conformational flexibility, intracellular ATP levels, and role of time and site of access. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 15-29 20556576-1 2010 Over-expression of P-glycoprotein (P-gp), an ATP-dependent drug efflux pump, represents one of the major mechanisms that contribute to multidrug resistance (MDR) in cancer cells. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 19-33 20556576-1 2010 Over-expression of P-glycoprotein (P-gp), an ATP-dependent drug efflux pump, represents one of the major mechanisms that contribute to multidrug resistance (MDR) in cancer cells. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 35-39 20071452-5 2010 Excellent signal/noise ratios of ATP-stimulated uptake for P-gp, MRP2, and BCRP vesicles were obtained, indicating high expression of functioning transporters. Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 20071452-7 2010 The ATP-dependent transport was inhibited by the model inhibitors verapamil (P-gp), benzbromarone (MRP2), and sulfasalazine (BCRP). Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 77-81 19915943-1 2010 P-glycoprotein (P-gp) on activated lymphocytes is an adenosine triphosphate (ATP)-binding cassette transporter that causes drug resistance by exclusion of intracellular drugs in patients with active rheumatoid arthritis (RA). Adenosine Triphosphate 53-75 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 19915943-1 2010 P-glycoprotein (P-gp) on activated lymphocytes is an adenosine triphosphate (ATP)-binding cassette transporter that causes drug resistance by exclusion of intracellular drugs in patients with active rheumatoid arthritis (RA). Adenosine Triphosphate 53-75 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 20104904-5 2010 Although 5-CHInd is an adenosine analogue that should block the binding of ATP, the non-natural nucleoside surprisingly stimulates the ATPase activity of P-gp in vitro. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 154-158 21133625-1 2010 The multidrug resistance (MDR1) gene product P-glycoprotein is a membrane bound protein that functions as an ATP-dependent efflux pump, transporting exogenous and endogenous substrates from the cells. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 26-30 19815037-6 2010 Furthermore, inhibition of Pgp functional activity also abolishes the effects of Pluronic on intracellular ATP levels in MDR cells suggesting that Pgp contributes to increased responsiveness of molecular "targets" of Pluronic in the mitochondria of MDR cells. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 27-30 19815037-6 2010 Furthermore, inhibition of Pgp functional activity also abolishes the effects of Pluronic on intracellular ATP levels in MDR cells suggesting that Pgp contributes to increased responsiveness of molecular "targets" of Pluronic in the mitochondria of MDR cells. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 147-150 19897579-4 2010 The ability of MRP1 to mediate ATP-dependent Cbl transport was confirmed by vesicular transport experiments, and a physiologic role of MRP1 in mammalian Cbl homeostasis is indicated by the phenotype of knockout mice with targeted disruption of MRP1. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 15-19 21133625-1 2010 The multidrug resistance (MDR1) gene product P-glycoprotein is a membrane bound protein that functions as an ATP-dependent efflux pump, transporting exogenous and endogenous substrates from the cells. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 45-59 20645921-3 2010 P-glycoprotein (P-gp/ABCB1) is the most important and studied member of such proteins belonging to the ATP Binding Cassette (ABC) superfamily of transporters that use ATP as energy source. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 20175745-2 2010 The multidrug resistance transporter P-glycoprotein (P-gp) is an ATP-dependent efflux pump and contributes to efflux of undesirable substances such as amyloid-beta:(Abeta) proteins from the brain into the blood as well as many drugs such as anti-cancer drugs. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 37-51 20175745-2 2010 The multidrug resistance transporter P-glycoprotein (P-gp) is an ATP-dependent efflux pump and contributes to efflux of undesirable substances such as amyloid-beta:(Abeta) proteins from the brain into the blood as well as many drugs such as anti-cancer drugs. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 53-57 20645921-3 2010 P-glycoprotein (P-gp/ABCB1) is the most important and studied member of such proteins belonging to the ATP Binding Cassette (ABC) superfamily of transporters that use ATP as energy source. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 21-26 19903372-12 2009 CONCLUSIONS: The contribution of ATP-dependent efflux transporters (MRP2 and P-gp) to the permeability of these compounds in the apical side could explain their low bioavailability. Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 77-81 19726755-1 2010 P-glycoprotein (Pgp) is an ATP-dependent efflux pump involved in transport of xenobiotics from cells that, when overexpressed, can mediate multidrug resistance in mammalian cells. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 19726755-1 2010 P-glycoprotein (Pgp) is an ATP-dependent efflux pump involved in transport of xenobiotics from cells that, when overexpressed, can mediate multidrug resistance in mammalian cells. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 19949922-2 2010 In particular efflux transporters of the ATP-binding cassette family such as ABCB1 (P-glycoprotein), the ABCC (multidrug resistance-associated protein) family, and ABCG2 (breast cancer resistance protein) have been identified as major determinants of chemoresistance in tumor cells. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 77-82 19949922-2 2010 In particular efflux transporters of the ATP-binding cassette family such as ABCB1 (P-glycoprotein), the ABCC (multidrug resistance-associated protein) family, and ABCG2 (breast cancer resistance protein) have been identified as major determinants of chemoresistance in tumor cells. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 84-98 20641310-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 20641310-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 39-53 20641310-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 55-59 19818729-1 2009 P-glycoprotein (ABCB1) is an ATP-driven efflux pump which binds drugs within a large flexible binding pocket. Adenosine Triphosphate 29-32 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 19818729-1 2009 P-glycoprotein (ABCB1) is an ATP-driven efflux pump which binds drugs within a large flexible binding pocket. Adenosine Triphosphate 29-32 ATP binding cassette subfamily B member 1 Homo sapiens 16-21 19952421-5 2009 The P-gp activity in the presence and absence of commonly used 50 Kampo medicines was evaluated by the ATPase assay detecting the inorganic phosphate produced by the ATP hydrolysis. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 19762091-2 2009 When cancer cells develop resistance to chemotherapeutics, it is frequently conferred by the ATP-dependent efflux pump P-glycoprotein (MDR1, P-gp, ABCB1). Adenosine Triphosphate 93-96 ATP binding cassette subfamily B member 1 Homo sapiens 135-139 19762091-2 2009 When cancer cells develop resistance to chemotherapeutics, it is frequently conferred by the ATP-dependent efflux pump P-glycoprotein (MDR1, P-gp, ABCB1). Adenosine Triphosphate 93-96 ATP binding cassette subfamily B member 1 Homo sapiens 147-152 19285030-3 2009 We report here that a GST linked NBD1(642-890)-GST-NBD2(1286-1531) was able to hydrolyze ATP at a rate of about 4.6 nmol/mg/min (K(m)=2.17 mM, V(max)=12.36 nmol/mg/min), which was comparable to the purified and reconstituted MRP1. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 225-229 19581304-1 2009 P-glycoprotein (P-gp, ATP-binding cassette B1) is a drug pump that extracts toxic drug substrates from the plasma membrane and catalyzes their ATP-dependent efflux. Adenosine Triphosphate 22-25 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 19581304-1 2009 P-glycoprotein (P-gp, ATP-binding cassette B1) is a drug pump that extracts toxic drug substrates from the plasma membrane and catalyzes their ATP-dependent efflux. Adenosine Triphosphate 22-25 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 19456124-0 2009 Transmembrane helix 12 modulates progression of the ATP catalytic cycle in ABCB1. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 75-80 19456124-9 2009 Overall, the results indicate that TM12 plays a key role in the progression of the ATP hydrolytic cycle in ABCB1, even in the absence of the transported substrate. Adenosine Triphosphate 83-86 ATP binding cassette subfamily B member 1 Homo sapiens 107-112 19233288-1 2009 Multidrug resistance protein MDR1 (P-glycoprotein/ABCB1) is an ATP-dependent efflux pump for various cytotoxic agents, and is implicated in the resistance of human tumors to chemotherapeutic drugs. Adenosine Triphosphate 63-66 ATP binding cassette subfamily B member 1 Homo sapiens 29-33 19233288-1 2009 Multidrug resistance protein MDR1 (P-glycoprotein/ABCB1) is an ATP-dependent efflux pump for various cytotoxic agents, and is implicated in the resistance of human tumors to chemotherapeutic drugs. Adenosine Triphosphate 63-66 ATP binding cassette subfamily B member 1 Homo sapiens 35-55 19394750-1 2009 OBJECTIVE: To investigate the interaction of imatinib mesylate (IM) with the clinically relevant adenosine triphosphate-binding cassette efflux transporter MDR1 (ABCB1) in cells from patients with chronic myeloid leukemia (CML) and to explore whether inhibition of this transporter would improve IM"s efficacy in the elimination of CML CD34(+) cells by increasing cell-associated drug accumulation. Adenosine Triphosphate 97-119 ATP binding cassette subfamily B member 1 Homo sapiens 156-160 19394750-1 2009 OBJECTIVE: To investigate the interaction of imatinib mesylate (IM) with the clinically relevant adenosine triphosphate-binding cassette efflux transporter MDR1 (ABCB1) in cells from patients with chronic myeloid leukemia (CML) and to explore whether inhibition of this transporter would improve IM"s efficacy in the elimination of CML CD34(+) cells by increasing cell-associated drug accumulation. Adenosine Triphosphate 97-119 ATP binding cassette subfamily B member 1 Homo sapiens 162-167 19493273-1 2009 Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) inhibit the function of certain adenosine triphosphate (ATP)-binding cassette transporters, including P-glycoprotein/ABCB1 and breast cancer resistance protein (BCRP)/ABCG2. Adenosine Triphosphate 104-126 ATP binding cassette subfamily B member 1 Homo sapiens 189-194 19493273-1 2009 Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) inhibit the function of certain adenosine triphosphate (ATP)-binding cassette transporters, including P-glycoprotein/ABCB1 and breast cancer resistance protein (BCRP)/ABCG2. Adenosine Triphosphate 128-131 ATP binding cassette subfamily B member 1 Homo sapiens 189-194 19490125-1 2009 Human P-glycoprotein (P-gp), which conveys multidrug resistance, is an ATP-dependent drug efflux pump that transports a wide variety of structurally unrelated compounds out of cells. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 6-20 19490125-1 2009 Human P-glycoprotein (P-gp), which conveys multidrug resistance, is an ATP-dependent drug efflux pump that transports a wide variety of structurally unrelated compounds out of cells. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 19688973-1 2009 AIM: P-glycoprotein, the product of the ATP-binding cassette subfamily B member 1 (ABCB1) gene (or the so-called multidrug resistance 1 gene), is an ATP-driven efflux pump contributing to the pharmacokinetics as well as the pharmacokinetics of drugs that are P-glycoprotein substrates, such as tacrolimus. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 5-19 19688973-1 2009 AIM: P-glycoprotein, the product of the ATP-binding cassette subfamily B member 1 (ABCB1) gene (or the so-called multidrug resistance 1 gene), is an ATP-driven efflux pump contributing to the pharmacokinetics as well as the pharmacokinetics of drugs that are P-glycoprotein substrates, such as tacrolimus. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 83-88 19688973-1 2009 AIM: P-glycoprotein, the product of the ATP-binding cassette subfamily B member 1 (ABCB1) gene (or the so-called multidrug resistance 1 gene), is an ATP-driven efflux pump contributing to the pharmacokinetics as well as the pharmacokinetics of drugs that are P-glycoprotein substrates, such as tacrolimus. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 113-135 19688973-1 2009 AIM: P-glycoprotein, the product of the ATP-binding cassette subfamily B member 1 (ABCB1) gene (or the so-called multidrug resistance 1 gene), is an ATP-driven efflux pump contributing to the pharmacokinetics as well as the pharmacokinetics of drugs that are P-glycoprotein substrates, such as tacrolimus. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 259-273 19215299-0 2009 Data-driven homology modelling of P-glycoprotein in the ATP-bound state indicates flexibility of the transmembrane domains. Adenosine Triphosphate 56-59 ATP binding cassette subfamily B member 1 Homo sapiens 34-48 20641209-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 4-9 20641209-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 39-53 20641209-1 2004 The MDR-1 gene encodes a transmembrane P-glycoprotein (P-gp) as an ATP-dependent multidrug transporter that is capable of actively pumping a variety of agents out of the cells. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 55-59 19159494-1 2009 BACKGROUND: P-glycoprotein belongs to the family of ATP-binding cassette proteins which hydrolyze ATP to catalyse the translocation of their substrates through membranes. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 12-26 18945821-1 2009 P-glycoprotein (P-gp), an ATP-dependent drug efflux pump, has been implicated in multidrug resistance of several cancers as a result of its overexpression. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 19049391-2 2008 One cause of MDR is the expression at the tumor cell surface of P-glycoprotein (Pgp), which functions as an ATP-powered multidrug efflux pump. Adenosine Triphosphate 108-111 ATP binding cassette subfamily B member 1 Homo sapiens 64-78 19049391-2 2008 One cause of MDR is the expression at the tumor cell surface of P-glycoprotein (Pgp), which functions as an ATP-powered multidrug efflux pump. Adenosine Triphosphate 108-111 ATP binding cassette subfamily B member 1 Homo sapiens 80-83 18930752-5 2008 Analysis of the expression of the ATP binding cassette efflux pumps MDR1, MRP2 and BCRP confirmed their apical membrane localisation. Adenosine Triphosphate 34-37 ATP binding cassette subfamily B member 1 Homo sapiens 68-72 18981575-1 2008 This study investigated the changes in the mRNA levels of the ATP binding cassette (ABC) transporters multidrug resistance 1 (MDR1), multidrug resistance-associated protein 1 (MRP1), and multidrug resistance-associated protein 2 (MRP2) following exposure to the prototypical microsomal enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) in primary cultures of cryopreserved human and cynomolgus monkey hepatocytes. Adenosine Triphosphate 62-65 ATP binding cassette subfamily B member 1 Homo sapiens 102-124 18945821-1 2009 P-glycoprotein (P-gp), an ATP-dependent drug efflux pump, has been implicated in multidrug resistance of several cancers as a result of its overexpression. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 18981575-1 2008 This study investigated the changes in the mRNA levels of the ATP binding cassette (ABC) transporters multidrug resistance 1 (MDR1), multidrug resistance-associated protein 1 (MRP1), and multidrug resistance-associated protein 2 (MRP2) following exposure to the prototypical microsomal enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) in primary cultures of cryopreserved human and cynomolgus monkey hepatocytes. Adenosine Triphosphate 62-65 ATP binding cassette subfamily B member 1 Homo sapiens 126-130 18723777-1 2008 The human multidrug resistance gene MDR1 encodes a membrane-bound transporter P-glycoprotein (Pgp) that confers the drug resistance of cancer cells by mediating an ATP-dependent drug efflux transport. Adenosine Triphosphate 164-167 ATP binding cassette subfamily B member 1 Homo sapiens 36-40 18617601-7 2008 The ATP-dependent uptake of rosuvastatin by human BCRP-expressing membrane vesicles was significantly higher than the uptake by green fluorescent protein-expressing control vesicles, suggesting that MRP2, MDR1, and BCRP can transport rosuvastatin. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 205-209 18708637-1 2008 P-glycoprotein (P-gp, ABCB1) is an ATP-dependent drug pump. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 18708637-1 2008 P-glycoprotein (P-gp, ABCB1) is an ATP-dependent drug pump. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 18708637-1 2008 P-glycoprotein (P-gp, ABCB1) is an ATP-dependent drug pump. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 22-27 18768387-1 2008 Multidrug resistance protein 1 (MRP1/ABCC1) is an ATP-dependent polytopic membrane protein that transports many anticancer drugs and organic anions. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 0-30 18336795-1 2008 The ATP-binding cassette multidrug resistance protein 1 (MRP1) mediates ATP-dependent cellular efflux of drugs and organic anions. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 25-55 18772522-3 2008 Among the transporter members of this family, ATP-dependent efflux transporter P-glycoprotein (MDR1) and organic anion transporters (OATP) are the most important proteins involved in drug transport. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 95-99 18588278-2 2008 The drug efflux system"s direct and indirect inhibition mediated by polymer P-glycoprotein (Pgp) interactions or adenosine triphosphate (ATP) depletion, respectively, may be involved in MDR reversal as well as damage to the membrane barrier caused by polymer insertion into the membrane. Adenosine Triphosphate 113-135 ATP binding cassette subfamily B member 1 Homo sapiens 76-90 18588278-2 2008 The drug efflux system"s direct and indirect inhibition mediated by polymer P-glycoprotein (Pgp) interactions or adenosine triphosphate (ATP) depletion, respectively, may be involved in MDR reversal as well as damage to the membrane barrier caused by polymer insertion into the membrane. Adenosine Triphosphate 137-140 ATP binding cassette subfamily B member 1 Homo sapiens 76-90 18633246-3 2008 P-glycoprotein(P-gp)is the one of the efflux adenosine triphosphate(ATP)-binding cassette family transporters and is the encoded product of MDR1 gene. Adenosine Triphosphate 45-67 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 18633246-3 2008 P-glycoprotein(P-gp)is the one of the efflux adenosine triphosphate(ATP)-binding cassette family transporters and is the encoded product of MDR1 gene. Adenosine Triphosphate 45-67 ATP binding cassette subfamily B member 1 Homo sapiens 140-144 18633246-3 2008 P-glycoprotein(P-gp)is the one of the efflux adenosine triphosphate(ATP)-binding cassette family transporters and is the encoded product of MDR1 gene. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 18633246-3 2008 P-glycoprotein(P-gp)is the one of the efflux adenosine triphosphate(ATP)-binding cassette family transporters and is the encoded product of MDR1 gene. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 140-144 18336795-1 2008 The ATP-binding cassette multidrug resistance protein 1 (MRP1) mediates ATP-dependent cellular efflux of drugs and organic anions. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 57-61 18336795-2 2008 We previously described a mutant, MRP1-Pro1150Ala, which exhibits selectively increased estradiol glucuronide (E217betaG) and methotrexate transport as well as altered interactions with ATP. Adenosine Triphosphate 186-189 ATP binding cassette subfamily B member 1 Homo sapiens 34-38 18035039-2 2008 In common with most ABC transporters, P-gp is comprised of two transmembrane domains (TMDs) and two nucleotide binding domains (NBD), the latter coupling ATP hydrolysis with substrate transport (efflux in the case of P-gp). Adenosine Triphosphate 154-157 ATP binding cassette subfamily B member 1 Homo sapiens 38-42 18093977-1 2008 P-glycoprotein (Pgp) is an ATP hydrolysis driven multidrug efflux pump, which, when overexpressed in the plasma membrane of certain cancers, can lead to the failure of chemotherapy. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 18093977-1 2008 P-glycoprotein (Pgp) is an ATP hydrolysis driven multidrug efflux pump, which, when overexpressed in the plasma membrane of certain cancers, can lead to the failure of chemotherapy. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 18190907-8 2008 ATPase activity determined by a colorimetric assay revealed that flurazepam inhibits the P-glycoprotein enzymatic activity, indicating coupling between drug transport and ATP hydrolysis. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 89-103 18035039-2 2008 In common with most ABC transporters, P-gp is comprised of two transmembrane domains (TMDs) and two nucleotide binding domains (NBD), the latter coupling ATP hydrolysis with substrate transport (efflux in the case of P-gp). Adenosine Triphosphate 154-157 ATP binding cassette subfamily B member 1 Homo sapiens 217-221 23100919-1 2007 The multidrug resistance 1 (MDR1) gene product, P-glycoprotein (Pgp/p170) is a membrane protein, which acts as an ATP dependant efflux pump that expels a wide variety of organic compounds including chemotherapeutic agents from the cell. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 4-26 18289002-2 2008 The phenomenon, termed multidrug resistance (MDR), is often associated with the over-expression of P-glycoprotein, a transmembrane protein pump, which can enhance efflux of a various chemicals structurally unrelated at the expense of ATP depletion, resulting in decrease of the intracellular cytotoxic drug accumulation. Adenosine Triphosphate 234-237 ATP binding cassette subfamily B member 1 Homo sapiens 99-113 17988154-0 2007 Catalytic cycle of ATP hydrolysis by P-glycoprotein: evidence for formation of the E.S reaction intermediate with ATP-gamma-S, a nonhydrolyzable analogue of ATP. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 37-51 17988154-0 2007 Catalytic cycle of ATP hydrolysis by P-glycoprotein: evidence for formation of the E.S reaction intermediate with ATP-gamma-S, a nonhydrolyzable analogue of ATP. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 37-51 17988154-2 2007 Moreover, an asymmetric occlusion of ATP at one of the two ATP sites of P-glycoprotein (Pgp) may follow the formation of the symmetric dimer. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 72-86 17988154-2 2007 Moreover, an asymmetric occlusion of ATP at one of the two ATP sites of P-glycoprotein (Pgp) may follow the formation of the symmetric dimer. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 88-91 17988154-2 2007 Moreover, an asymmetric occlusion of ATP at one of the two ATP sites of P-glycoprotein (Pgp) may follow the formation of the symmetric dimer. Adenosine Triphosphate 59-62 ATP binding cassette subfamily B member 1 Homo sapiens 72-86 17988154-2 2007 Moreover, an asymmetric occlusion of ATP at one of the two ATP sites of P-glycoprotein (Pgp) may follow the formation of the symmetric dimer. Adenosine Triphosphate 59-62 ATP binding cassette subfamily B member 1 Homo sapiens 88-91 17988154-4 2007 In this study, we show that the E.S conformation of Pgp (previously demonstrated in the E556Q/E1201Q mutant Pgp) can be obtained with the wild-type protein by use of the nonhydrolyzable ATP analogue ATP-gamma-S. Adenosine Triphosphate 186-189 ATP binding cassette subfamily B member 1 Homo sapiens 52-55 17988154-4 2007 In this study, we show that the E.S conformation of Pgp (previously demonstrated in the E556Q/E1201Q mutant Pgp) can be obtained with the wild-type protein by use of the nonhydrolyzable ATP analogue ATP-gamma-S. Adenosine Triphosphate 186-189 ATP binding cassette subfamily B member 1 Homo sapiens 108-111 17988154-5 2007 ATP-gamma-S is occluded into the Pgp NBDs at 34 degrees C but not at 4 degrees C, whereas ATP is not occluded at either temperature. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 33-36 23100919-1 2007 The multidrug resistance 1 (MDR1) gene product, P-glycoprotein (Pgp/p170) is a membrane protein, which acts as an ATP dependant efflux pump that expels a wide variety of organic compounds including chemotherapeutic agents from the cell. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 17803828-3 2007 RESULTS: In order to elucidate structural and molecular concepts of multidrug resistance, we have constructed a molecular model of the ATP-bound outward facing conformation of the human multidrug resistance protein ABCB1 using the Sav1866 crystal structure as a template, and compared the ABCB1 model with a previous ABCC5 model. Adenosine Triphosphate 135-138 ATP binding cassette subfamily B member 1 Homo sapiens 215-220 17912240-1 2007 P-glycoprotein (P-gp) pumps multiple types of drugs out of the cell, using energy generated from ATP, and confers multidrug resistance (MDR) on cancer cells. Adenosine Triphosphate 97-100 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 17912240-1 2007 P-glycoprotein (P-gp) pumps multiple types of drugs out of the cell, using energy generated from ATP, and confers multidrug resistance (MDR) on cancer cells. Adenosine Triphosphate 97-100 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 17803828-3 2007 RESULTS: In order to elucidate structural and molecular concepts of multidrug resistance, we have constructed a molecular model of the ATP-bound outward facing conformation of the human multidrug resistance protein ABCB1 using the Sav1866 crystal structure as a template, and compared the ABCB1 model with a previous ABCC5 model. Adenosine Triphosphate 135-138 ATP binding cassette subfamily B member 1 Homo sapiens 289-294 17958337-1 2007 P-glycoprotein, an ATP-dependent efflux pump, is a membrane transporter that influences the absorption and excretion of drugs. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 17692467-2 2007 Pgp is a heavily glycosylated, ATP-dependent drug efflux pump expressed in many human cancers. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 17636884-0 2007 Nucleotide binding, ATP hydrolysis, and mutation of the catalytic carboxylates of human P-glycoprotein cause distinct conformational changes in the transmembrane segments. Adenosine Triphosphate 20-23 ATP binding cassette subfamily B member 1 Homo sapiens 88-102 17548681-1 2007 OBJECTIVE: The multidrug resistance (MDR) 1 gene encodes a membrane transporter called P-glycoprotein, which plays an important role in protecting cells against lipophilic xenobiotics by way of an ATP-dependent cellular efflux mechanism. Adenosine Triphosphate 197-200 ATP binding cassette subfamily B member 1 Homo sapiens 15-43 17134887-1 2007 P-glycoprotein (P-gp) is involved in the ATP-dependant cellular efflux of a large number of drugs including ivermectin, a macrocyclic lactone (ML) endectocide, widely used in livestock and human antiparasitic therapy. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 17428165-3 2007 Tariquidar inhibits the ATPase activity of P-glycoprotein, suggesting that the modulating effect is derived from the inhibition of substrate binding, inhibition of ATP hydrolysis or both. Adenosine Triphosphate 24-27 ATP binding cassette subfamily B member 1 Homo sapiens 43-57 17213652-3 2007 Berberine accumulation depended on the cellular ATP level, and was reversed by typical inhibitors of MDR1, suggesting that human MDR1 and MRP1 directly efflux berberine as their substrate. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 129-133 17402598-2 2007 Being an ATP-dependent transport agent in the reverse yield, P-glycoprotein participates in the intestinal secretion, limitation of the permeability of histohematic barriers, and the renal and bile excretion of exogenous substrates, thus protecting the organism against xenobiotics. Adenosine Triphosphate 9-12 ATP binding cassette subfamily B member 1 Homo sapiens 61-75 16919386-0 2007 Sensitivity of P-glycoprotein tryptophan residues to benzodiazepines and ATP interaction. Adenosine Triphosphate 73-76 ATP binding cassette subfamily B member 1 Homo sapiens 15-29 16919386-5 2007 Furthermore, quenching data of benzodiazepine-bound P-glycoprotein with ATP were concentration dependent and saturable, indicating that nucleotide binds to P-glycoprotein whether drug is present or not. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 52-66 16919386-5 2007 Furthermore, quenching data of benzodiazepine-bound P-glycoprotein with ATP were concentration dependent and saturable, indicating that nucleotide binds to P-glycoprotein whether drug is present or not. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 156-170 17134887-1 2007 P-glycoprotein (P-gp) is involved in the ATP-dependant cellular efflux of a large number of drugs including ivermectin, a macrocyclic lactone (ML) endectocide, widely used in livestock and human antiparasitic therapy. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 17215884-1 2006 P-glycoprotein (Pgp; ABCB1), a member of the ATP-binding cassette (ABC) superfamily, exports structurally diverse hydrophobic compounds from the cell, driven by ATP hydrolysis. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 17237262-0 2007 About a switch: how P-glycoprotein (ABCB1) harnesses the energy of ATP binding and hydrolysis to do mechanical work. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 20-34 17237262-0 2007 About a switch: how P-glycoprotein (ABCB1) harnesses the energy of ATP binding and hydrolysis to do mechanical work. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 36-41 17237262-7 2007 Based on the mutational and biochemical work on Pgp and structural studies with isolated NBDs, we review proposed schemes for the catalytic cycle of ATP hydrolysis and the transport pathway. Adenosine Triphosphate 149-152 ATP binding cassette subfamily B member 1 Homo sapiens 48-51 17095241-1 2007 P-glycoprotein (P-gp) the multidrug transporter is a well-characterised member of the super-family of ATP-binding cassette (ABC) transporters, and mediates the clearance of xenotoxins against steep concentration gradients at the expense of ATP hydrolysis. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 17095241-1 2007 P-glycoprotein (P-gp) the multidrug transporter is a well-characterised member of the super-family of ATP-binding cassette (ABC) transporters, and mediates the clearance of xenotoxins against steep concentration gradients at the expense of ATP hydrolysis. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 17215884-1 2006 P-glycoprotein (Pgp; ABCB1), a member of the ATP-binding cassette (ABC) superfamily, exports structurally diverse hydrophobic compounds from the cell, driven by ATP hydrolysis. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 17215884-1 2006 P-glycoprotein (Pgp; ABCB1), a member of the ATP-binding cassette (ABC) superfamily, exports structurally diverse hydrophobic compounds from the cell, driven by ATP hydrolysis. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 21-26 17142983-1 2006 Human ABC transporter P-glycoprotein (P-gp/ABCB1) encoded by the multidrug resistance (MDR1) gene is recognized as one of the most important factors regulating pharmacokinetics of a number of clinically important drugs because of its function of extruding a wide range of structurally unrelated amphiphilic and hydrophobic drugs from the inside to the outside of cells in an ATP-driven mechanism. Adenosine Triphosphate 375-378 ATP binding cassette subfamily B member 1 Homo sapiens 22-36 17142983-1 2006 Human ABC transporter P-glycoprotein (P-gp/ABCB1) encoded by the multidrug resistance (MDR1) gene is recognized as one of the most important factors regulating pharmacokinetics of a number of clinically important drugs because of its function of extruding a wide range of structurally unrelated amphiphilic and hydrophobic drugs from the inside to the outside of cells in an ATP-driven mechanism. Adenosine Triphosphate 375-378 ATP binding cassette subfamily B member 1 Homo sapiens 43-48 17142983-1 2006 Human ABC transporter P-glycoprotein (P-gp/ABCB1) encoded by the multidrug resistance (MDR1) gene is recognized as one of the most important factors regulating pharmacokinetics of a number of clinically important drugs because of its function of extruding a wide range of structurally unrelated amphiphilic and hydrophobic drugs from the inside to the outside of cells in an ATP-driven mechanism. Adenosine Triphosphate 375-378 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 16803457-5 2006 Trapping with V(i) and ATP led to a 6-fold increase in the acrylamide quenching constant, K(SV), suggesting that large conformational changes take place in the Pgp transmembrane regions on trapping in the forward direction. Adenosine Triphosphate 23-26 ATP binding cassette subfamily B member 1 Homo sapiens 160-163 16801529-1 2006 Transport of several xenobiotics including pharmacological agents into or out of the central nervous system (CNS) involves the expression of ATP-dependent, membrane-bound efflux transport proteins such as P-glycoprotein (P-gp) at the blood-brain barrier (BBB). Adenosine Triphosphate 141-144 ATP binding cassette subfamily B member 1 Homo sapiens 205-219 16801529-1 2006 Transport of several xenobiotics including pharmacological agents into or out of the central nervous system (CNS) involves the expression of ATP-dependent, membrane-bound efflux transport proteins such as P-glycoprotein (P-gp) at the blood-brain barrier (BBB). Adenosine Triphosphate 141-144 ATP binding cassette subfamily B member 1 Homo sapiens 221-225 16861249-5 2006 The mutation of either ICL5 or ICL7 considerably decreased ATP-dependent LTC4 uptake into vesicles of insect cells expressing mutated MRP1. Adenosine Triphosphate 59-62 ATP binding cassette subfamily B member 1 Homo sapiens 134-138 16917872-2 2006 P-gp is an integral membrane protein which mediates ATP-dependent substrate efflux. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 16803457-1 2006 The Pgp (P-glycoprotein) multidrug transporter couples ATP hydrolysis at two cytoplasmic NBDs (nucleotide-binding domains) to the transport of hydrophobic compounds. Adenosine Triphosphate 55-58 ATP binding cassette subfamily B member 1 Homo sapiens 4-7 16803457-1 2006 The Pgp (P-glycoprotein) multidrug transporter couples ATP hydrolysis at two cytoplasmic NBDs (nucleotide-binding domains) to the transport of hydrophobic compounds. Adenosine Triphosphate 55-58 ATP binding cassette subfamily B member 1 Homo sapiens 9-23 16803457-3 2006 We studied the trapped complexes formed via incubation of Pgp with ATP (catalytically forward) or ADP (reverse) and V(i), BeF(x) or AlF(x) using Mg2+ or Co2+ as the bivalent cation. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 58-61 16837625-1 2006 The ATP-driven drug export pump, P-glycoprotein, is a primary gatekeeper of the blood-brain barrier and a major impediment to central nervous system (CNS) pharmacotherapy. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 33-47 16800628-1 2006 The multidrug resistance efflux pump P-glycoprotein (Pgp) couples drug export to ATP binding and hydrolysis. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 37-51 16753135-5 2006 DR5 but not DR4 engagement by TRAIL attenuated cellular ATP levels by robustly stimulating P-gp ATPase activity, and thus triggered P-gp-dependent apoptosis by depletion of the cellular ATP pool. Adenosine Triphosphate 96-99 ATP binding cassette subfamily B member 1 Homo sapiens 91-95 16753135-6 2006 In addition to hyperactive P-gp-mediated ATP hydrolysis, TRAIL-induced, P-gp-potentiated apoptosis was associated with activation of caspases-6, -7, -8, and -9; Bid cleavage; and mitochondrial depolarization. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 27-31 16800628-1 2006 The multidrug resistance efflux pump P-glycoprotein (Pgp) couples drug export to ATP binding and hydrolysis. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 53-56 16800628-9 2006 Using established methods involving ADP-Vi trapping by wild-type Pgp and ATP binding by catalytic carboxylate mutant Pgp, these effects can be extended to ATP hydrolysis transition-state stabilization and ATP occlusion at a single site. Adenosine Triphosphate 73-76 ATP binding cassette subfamily B member 1 Homo sapiens 117-120 16800628-9 2006 Using established methods involving ADP-Vi trapping by wild-type Pgp and ATP binding by catalytic carboxylate mutant Pgp, these effects can be extended to ATP hydrolysis transition-state stabilization and ATP occlusion at a single site. Adenosine Triphosphate 155-158 ATP binding cassette subfamily B member 1 Homo sapiens 65-68 16800628-9 2006 Using established methods involving ADP-Vi trapping by wild-type Pgp and ATP binding by catalytic carboxylate mutant Pgp, these effects can be extended to ATP hydrolysis transition-state stabilization and ATP occlusion at a single site. Adenosine Triphosphate 155-158 ATP binding cassette subfamily B member 1 Homo sapiens 117-120 16800628-9 2006 Using established methods involving ADP-Vi trapping by wild-type Pgp and ATP binding by catalytic carboxylate mutant Pgp, these effects can be extended to ATP hydrolysis transition-state stabilization and ATP occlusion at a single site. Adenosine Triphosphate 155-158 ATP binding cassette subfamily B member 1 Homo sapiens 65-68 16800628-9 2006 Using established methods involving ADP-Vi trapping by wild-type Pgp and ATP binding by catalytic carboxylate mutant Pgp, these effects can be extended to ATP hydrolysis transition-state stabilization and ATP occlusion at a single site. Adenosine Triphosphate 155-158 ATP binding cassette subfamily B member 1 Homo sapiens 117-120 16551273-0 2006 Replacement of the positively charged Walker A lysine residue with a hydrophobic leucine residue and conformational alterations caused by this mutation in MRP1 impair ATP binding and hydrolysis. Adenosine Triphosphate 167-170 ATP binding cassette subfamily B member 1 Homo sapiens 155-159 16551273-1 2006 MRP1 (multidrug resistance protein 1) couples ATP binding/hydrolysis at its two non-equivalent NBDs (nucleotide-binding domains) with solute transport. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 16551273-1 2006 MRP1 (multidrug resistance protein 1) couples ATP binding/hydrolysis at its two non-equivalent NBDs (nucleotide-binding domains) with solute transport. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 6-36 16784241-6 2006 MRP1 mediated efficient ATP-dependent transport of LNO(2)-SG. Adenosine Triphosphate 24-27 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 16565061-4 2006 Utilizing the energy of ATP hydrolysis, P-glycoprotein is thought to take up substrates from the cytoplasmic leaflet of the plasma membrane and to transport them to the outside of the cell. Adenosine Triphosphate 24-27 ATP binding cassette subfamily B member 1 Homo sapiens 40-54 16768456-0 2006 The conserved tyrosine residues 401 and 1044 in ATP sites of human P-glycoprotein are critical for ATP binding and hydrolysis: evidence for a conserved subdomain, the A-loop in the ATP-binding cassette. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 16768456-0 2006 The conserved tyrosine residues 401 and 1044 in ATP sites of human P-glycoprotein are critical for ATP binding and hydrolysis: evidence for a conserved subdomain, the A-loop in the ATP-binding cassette. Adenosine Triphosphate 99-102 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 16697012-0 2006 Structure of the human multidrug resistance protein 1 nucleotide binding domain 1 bound to Mg2+/ATP reveals a non-productive catalytic site. Adenosine Triphosphate 96-99 ATP binding cassette subfamily B member 1 Homo sapiens 23-53 16697012-5 2006 Contrary to the prokaryotic NBDs, the extremely low intrinsic ATPase activity of isolated MRP1-NBDs allowed us to obtain the structure of wild-type NBD1 in complex with Mg2+/ATP. Adenosine Triphosphate 62-65 ATP binding cassette subfamily B member 1 Homo sapiens 90-94 16848721-3 2006 One major mechanism by which this occurs is through the over-expression of ATP-dependent drug efflux transporters such as the P-glycoprotein (PGP) and multidrug resistance-associated protein (MRP). Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 126-140 16848721-3 2006 One major mechanism by which this occurs is through the over-expression of ATP-dependent drug efflux transporters such as the P-glycoprotein (PGP) and multidrug resistance-associated protein (MRP). Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 142-145 16504381-2 2006 It is now widely appreciated that expression of the ATP-dependent efflux transporter, MDR1 (ABCB1, P-glycoprotein), in organs such as the gastrointestinal tract, liver and kidney significantly alters the extent of drug absorption and excretion. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 86-90 16505485-0 2006 Allosteric modulation bypasses the requirement for ATP hydrolysis in regenerating low affinity transition state conformation of human P-glycoprotein. Adenosine Triphosphate 51-54 ATP binding cassette subfamily B member 1 Homo sapiens 134-148 16505485-1 2006 ATP-dependent drug transport by human P-glycoprotein (Pgp, ABCB1) involves a coordinated communication between its drug-binding site (substrate site) and the nucleotide binding/hydrolysis domain (ATP sites). Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 38-52 16505485-1 2006 ATP-dependent drug transport by human P-glycoprotein (Pgp, ABCB1) involves a coordinated communication between its drug-binding site (substrate site) and the nucleotide binding/hydrolysis domain (ATP sites). Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 54-57 16505485-1 2006 ATP-dependent drug transport by human P-glycoprotein (Pgp, ABCB1) involves a coordinated communication between its drug-binding site (substrate site) and the nucleotide binding/hydrolysis domain (ATP sites). Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 59-64 16505485-1 2006 ATP-dependent drug transport by human P-glycoprotein (Pgp, ABCB1) involves a coordinated communication between its drug-binding site (substrate site) and the nucleotide binding/hydrolysis domain (ATP sites). Adenosine Triphosphate 196-199 ATP binding cassette subfamily B member 1 Homo sapiens 38-52 16505485-1 2006 ATP-dependent drug transport by human P-glycoprotein (Pgp, ABCB1) involves a coordinated communication between its drug-binding site (substrate site) and the nucleotide binding/hydrolysis domain (ATP sites). Adenosine Triphosphate 196-199 ATP binding cassette subfamily B member 1 Homo sapiens 54-57 16505485-1 2006 ATP-dependent drug transport by human P-glycoprotein (Pgp, ABCB1) involves a coordinated communication between its drug-binding site (substrate site) and the nucleotide binding/hydrolysis domain (ATP sites). Adenosine Triphosphate 196-199 ATP binding cassette subfamily B member 1 Homo sapiens 59-64 16505485-2 2006 It has been demonstrated that the two ATP sites of Pgp play distinct roles within a single catalytic turnover; whereas ATP binding or/and hydrolysis by one drives substrate translocation and dissociation, the hydrolytic activity of the other resets the transporter for the subsequent cycle (Sauna, Z. E., and Ambudkar, S. V. (2000) Proc. Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 51-54 16505485-2 2006 It has been demonstrated that the two ATP sites of Pgp play distinct roles within a single catalytic turnover; whereas ATP binding or/and hydrolysis by one drives substrate translocation and dissociation, the hydrolytic activity of the other resets the transporter for the subsequent cycle (Sauna, Z. E., and Ambudkar, S. V. (2000) Proc. Adenosine Triphosphate 119-122 ATP binding cassette subfamily B member 1 Homo sapiens 51-54 16352426-4 2006 There is strong biochemical evidence that Pgp moves molecular cargo against a concentration gradient using the energy of ATP hydrolysis. Adenosine Triphosphate 121-124 ATP binding cassette subfamily B member 1 Homo sapiens 42-45 16352426-9 2006 This review considers: (i) the role of specific conserved amino acids in ATP hydrolysis mediated by Pgp; (ii) emerging insights into the dimensions of the drug binding pocket and the interactions between Pgp and the transport substrates and (iii) our current understanding of the mechanisms of coupling between energy derived from ATP binding and/or hydrolysis and efflux of drug substrates. Adenosine Triphosphate 73-76 ATP binding cassette subfamily B member 1 Homo sapiens 100-103 16352426-9 2006 This review considers: (i) the role of specific conserved amino acids in ATP hydrolysis mediated by Pgp; (ii) emerging insights into the dimensions of the drug binding pocket and the interactions between Pgp and the transport substrates and (iii) our current understanding of the mechanisms of coupling between energy derived from ATP binding and/or hydrolysis and efflux of drug substrates. Adenosine Triphosphate 331-334 ATP binding cassette subfamily B member 1 Homo sapiens 204-207 16565074-1 2006 Multidrug resistance protein 1 (MRP1/ABCC1) is an ATP-dependent efflux pump that can confer resistance to multiple anticancer drugs and transport conjugated organic anions. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 0-30 16434618-4 2006 Moreover, in vitro experiments using inside-out membrane vesicles show that MRP1 supports ATP-dependent, osmotically sensitive uptake of MX. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 76-80 16504381-2 2006 It is now widely appreciated that expression of the ATP-dependent efflux transporter, MDR1 (ABCB1, P-glycoprotein), in organs such as the gastrointestinal tract, liver and kidney significantly alters the extent of drug absorption and excretion. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 92-97 16504381-2 2006 It is now widely appreciated that expression of the ATP-dependent efflux transporter, MDR1 (ABCB1, P-glycoprotein), in organs such as the gastrointestinal tract, liver and kidney significantly alters the extent of drug absorption and excretion. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 99-113 16489767-3 2006 In this study, we demonstrate that certain thioxanthene-based Pgp modulators, such as cis-(Z)-flupentixol and its closely related analogues, effectively disrupt molecular cross talk between the substrate, and the ATP, sites without affecting the basic functional aspects of the two domains, such as substrate recognition, binding, and hydrolysis of ATP and dissociation of ADP following ATP hydrolysis. Adenosine Triphosphate 349-352 ATP binding cassette subfamily B member 1 Homo sapiens 62-65 16508168-1 2006 We investigated whether P-glycoprotein (P-gp) ATPase activity of Caco-2 cell membranes could be estimated by measuring consumption of ATP using luciferin-luciferase reaction, and whether the results would be useful for assessment of the interactions between P-gp and drugs. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 24-38 16508168-1 2006 We investigated whether P-glycoprotein (P-gp) ATPase activity of Caco-2 cell membranes could be estimated by measuring consumption of ATP using luciferin-luciferase reaction, and whether the results would be useful for assessment of the interactions between P-gp and drugs. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 40-44 16489767-0 2006 Modulator-induced interference in functional cross talk between the substrate and the ATP sites of human P-glycoprotein. Adenosine Triphosphate 86-89 ATP binding cassette subfamily B member 1 Homo sapiens 105-119 16489767-1 2006 The human P-glycoprotein (Pgp, ABCB1) is an ATP-dependent efflux pump for structurally unrelated hydrophobic compounds, conferring simultaneous resistance to and restricting bioavailability of several anticancer and antimicrobial agents. Adenosine Triphosphate 44-47 ATP binding cassette subfamily B member 1 Homo sapiens 10-24 16489767-1 2006 The human P-glycoprotein (Pgp, ABCB1) is an ATP-dependent efflux pump for structurally unrelated hydrophobic compounds, conferring simultaneous resistance to and restricting bioavailability of several anticancer and antimicrobial agents. Adenosine Triphosphate 44-47 ATP binding cassette subfamily B member 1 Homo sapiens 26-29 16489767-1 2006 The human P-glycoprotein (Pgp, ABCB1) is an ATP-dependent efflux pump for structurally unrelated hydrophobic compounds, conferring simultaneous resistance to and restricting bioavailability of several anticancer and antimicrobial agents. Adenosine Triphosphate 44-47 ATP binding cassette subfamily B member 1 Homo sapiens 31-36 16489767-2 2006 Drug transport by Pgp requires a coordinated communication between its substrate binding/translocating pathway (substrate site) and the nucleotide binding domains (NBDs or ATP sites). Adenosine Triphosphate 172-175 ATP binding cassette subfamily B member 1 Homo sapiens 18-21 16489767-3 2006 In this study, we demonstrate that certain thioxanthene-based Pgp modulators, such as cis-(Z)-flupentixol and its closely related analogues, effectively disrupt molecular cross talk between the substrate, and the ATP, sites without affecting the basic functional aspects of the two domains, such as substrate recognition, binding, and hydrolysis of ATP and dissociation of ADP following ATP hydrolysis. Adenosine Triphosphate 213-216 ATP binding cassette subfamily B member 1 Homo sapiens 62-65 16489767-3 2006 In this study, we demonstrate that certain thioxanthene-based Pgp modulators, such as cis-(Z)-flupentixol and its closely related analogues, effectively disrupt molecular cross talk between the substrate, and the ATP, sites without affecting the basic functional aspects of the two domains, such as substrate recognition, binding, and hydrolysis of ATP and dissociation of ADP following ATP hydrolysis. Adenosine Triphosphate 349-352 ATP binding cassette subfamily B member 1 Homo sapiens 62-65 16489767-5 2006 Both hydrolysis of ATP and vanadate-induced [alpha-(32)P]-8-azido-ADP trapping (following [alpha-(32)P]-8-azido-ATP breakdown) by Pgp are stimulated by the modulator. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 130-133 16489767-6 2006 However, the ability of Pgp substrates (such as prazosin) to stimulate ATP hydrolysis and facilitate vanadate-induced trapping of [alpha-(32)P]-8-azido-ADP is substantially affected in the presence of cis-(Z)-flupentixol. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 24-27 16489767-7 2006 Substrate recognition by Pgp as determined by [(125)I]iodoarylazidoprazosin ([(125)I]IAAP) binding both in the presence and in the absence of ATP is facilitated by the modulator, whereas substrate dissociation in response to vanadate trapping is considerably affected in its presence. Adenosine Triphosphate 142-145 ATP binding cassette subfamily B member 1 Homo sapiens 25-28 16489767-10 2006 Taken together, our results demonstrate a distinct mechanism of Pgp modulation that involves allosteric disruption of molecular cross talk between the substrate, and the ATP, sites without any direct interference with their individual functions. Adenosine Triphosphate 170-173 ATP binding cassette subfamily B member 1 Homo sapiens 64-67 16380120-4 2006 The translocation pathway is critically dependent on ATP hydrolysis and drug interaction with P-gp is possible at one of a multitude of allosterically linked binding sites. Adenosine Triphosphate 53-56 ATP binding cassette subfamily B member 1 Homo sapiens 94-98 16433039-1 2006 The resistance to chemotherapy of cancer cells is mediated by the overexpression of P-glycoprotein, as an ATP-dependent membrane efflux pump. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 84-98 16691487-1 2005 The MDR1 P-glycoprotein, an ATP-binding cassette (ABC) superfamily member that functions as an ATP-driven drug efflux pump, has been linked to resistance of human tumors to multiple chemotherapeutic agents. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 16273216-1 2005 Multidrug resistance (MDR) in tumor cells is generally associated with increased efflux of the cytotoxic compounds, due to the activation of mechanisms of intracellular transport and to the overexpression of surface proteins, such as P-glycoprotein (Pgp), which act as ATP-dependent molecular pumps. Adenosine Triphosphate 269-272 ATP binding cassette subfamily B member 1 Homo sapiens 234-248 16273216-1 2005 Multidrug resistance (MDR) in tumor cells is generally associated with increased efflux of the cytotoxic compounds, due to the activation of mechanisms of intracellular transport and to the overexpression of surface proteins, such as P-glycoprotein (Pgp), which act as ATP-dependent molecular pumps. Adenosine Triphosphate 269-272 ATP binding cassette subfamily B member 1 Homo sapiens 250-253 16554667-6 2006 It has also been suggested that ATP-dependent primary active transporters such as MDR1/P-glycoprotein and the multidrug resistance-associated protein family function as efflux pumps of renal tubular cells for more hydrophobic molecules and anionic conjugates. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 82-86 16554667-6 2006 It has also been suggested that ATP-dependent primary active transporters such as MDR1/P-glycoprotein and the multidrug resistance-associated protein family function as efflux pumps of renal tubular cells for more hydrophobic molecules and anionic conjugates. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 87-101 16691487-1 2005 The MDR1 P-glycoprotein, an ATP-binding cassette (ABC) superfamily member that functions as an ATP-driven drug efflux pump, has been linked to resistance of human tumors to multiple chemotherapeutic agents. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 9-23 16691488-1 2005 Human P-glycoprotein (ABCB1) is a primary multidrug transporter located in plasma membranes, that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Adenosine Triphosphate 121-124 ATP binding cassette subfamily B member 1 Homo sapiens 22-27 16105987-2 2005 In addition to their drug efflux properties, certain ABC proteins such as multidrug resistance protein 1 (MRP1) (ABCC1) mediate the ATP-dependent transport of a broad array of organic anions. Adenosine Triphosphate 132-135 ATP binding cassette subfamily B member 1 Homo sapiens 74-104 16309179-4 2005 The [delta32 P] ATP-driven phosphorylation of Pgp was strongly increased in OG-EX, decreased in F3 and not detected in F3-PLP, when compared to Pgp phosphorylation in native plasma membrane vesicles. Adenosine Triphosphate 16-19 ATP binding cassette subfamily B member 1 Homo sapiens 46-49 16309179-5 2005 [delta32 P]ATP-phosphorylation of Pgp in F3-PLP could be restored by exogenously added PKC or by the catalytic sub-unit of PKA. Adenosine Triphosphate 11-14 ATP binding cassette subfamily B member 1 Homo sapiens 34-37 16309179-6 2005 The vanadate-induced hyperphosphorylation effect on Pgp by [delta32 P]ATP observed with plasma membrane vesicles was maintained in OG-EX, but was lost in F3 and did not enable labelling in F3-PLP. Adenosine Triphosphate 70-73 ATP binding cassette subfamily B member 1 Homo sapiens 52-55 16309179-7 2005 Enhancement of [delta32 P]-labelling of native Pgp via [delta32 P]ATP combined with GTP was maintained and also triggered phosphorylation of purified/reconstituted Pgp in F3-PLP as well. Adenosine Triphosphate 66-69 ATP binding cassette subfamily B member 1 Homo sapiens 47-50 16309179-9 2005 In addition, restoration by GTP of Pgp phosphorylation by [delta32 P]ATP in the frame of F3-PLP suggests intra-molecular modulations and hints that other phosphorylation sites and processes, different from the classic ones involving PKC and/or PKA, may participate in the transporter"s mechanism. Adenosine Triphosphate 69-72 ATP binding cassette subfamily B member 1 Homo sapiens 35-38 16105987-2 2005 In addition to their drug efflux properties, certain ABC proteins such as multidrug resistance protein 1 (MRP1) (ABCC1) mediate the ATP-dependent transport of a broad array of organic anions. Adenosine Triphosphate 132-135 ATP binding cassette subfamily B member 1 Homo sapiens 106-110 15964250-0 2005 P-glycoprotein expression increases ATP release in respiratory cystic fibrosis cells. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 16229491-1 2005 The P-glycoprotein efflux pump, an ABC superfamily member, can export a wide variety of hydrophobic drugs, natural products, and peptides from cells, powered by the energy of ATP hydrolysis. Adenosine Triphosphate 175-178 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 16176266-1 2005 P-glycoprotein (Pgp), a member of the ATP-binding cassette (ABC) superfamily responsible for the ATP-driven extrusion of diverse hydrophobic molecules from cells, is a cause of multidrug resistance in human tumours. Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 16176266-1 2005 P-glycoprotein (Pgp), a member of the ATP-binding cassette (ABC) superfamily responsible for the ATP-driven extrusion of diverse hydrophobic molecules from cells, is a cause of multidrug resistance in human tumours. Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 15838832-0 2005 Probing ATP-dependent conformational changes in the multidrug resistance protein 1 (MRP1/ABCC1) in live tumor cells with a novel recombinant single-chain Fv antibody targeted to the extracellular N-terminus. Adenosine Triphosphate 8-11 ATP binding cassette subfamily B member 1 Homo sapiens 52-82 16159384-1 2005 BACKGROUND: P-glycoprotein is responsible for the ATP-dependent export of certain structurally unrelated compounds including many chemotherapeutic drugs. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 12-26 15964250-7 2005 We confirmed that overexpression of Pgp causes a significant increase in cellular ATP release, which could even be enhanced by stimulation with hypoosmolar medium. Adenosine Triphosphate 82-85 ATP binding cassette subfamily B member 1 Homo sapiens 36-39 16004994-4 2005 Covalent attachment of coumarin-maleimide at residue 339C within TM6 resulted in impaired ATP hydrolysis by P-gp. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 108-112 15955594-3 2005 P-glycoprotein (Pgp) also pumps multiple types of drugs out of the cell using energy generated from ATP, and confers multidrug resistance on cancer cells. Adenosine Triphosphate 100-103 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 15955594-3 2005 P-glycoprotein (Pgp) also pumps multiple types of drugs out of the cell using energy generated from ATP, and confers multidrug resistance on cancer cells. Adenosine Triphosphate 100-103 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 16042402-0 2005 ATP hydrolysis promotes interactions between the extracellular ends of transmembrane segments 1 and 11 of human multidrug resistance P-glycoprotein. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 133-147 16456713-1 2005 P-glycoprotein (P-gp) is an ATP-dependent drug pump that can transport a broad range of hydrophobic compounds out of the cell. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 16456713-1 2005 P-glycoprotein (P-gp) is an ATP-dependent drug pump that can transport a broad range of hydrophobic compounds out of the cell. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 15939500-2 2005 This effect is attributed to the inhibition of the most clinically relevant drug efflux transporter, P-glycoprotein (Pgp), through the combined ATP depletion and inhibition of Pgp ATPase activity. Adenosine Triphosphate 144-147 ATP binding cassette subfamily B member 1 Homo sapiens 101-115 15939500-2 2005 This effect is attributed to the inhibition of the most clinically relevant drug efflux transporter, P-glycoprotein (Pgp), through the combined ATP depletion and inhibition of Pgp ATPase activity. Adenosine Triphosphate 144-147 ATP binding cassette subfamily B member 1 Homo sapiens 117-120 15939500-2 2005 This effect is attributed to the inhibition of the most clinically relevant drug efflux transporter, P-glycoprotein (Pgp), through the combined ATP depletion and inhibition of Pgp ATPase activity. Adenosine Triphosphate 144-147 ATP binding cassette subfamily B member 1 Homo sapiens 176-179 15755910-5 2005 We have demonstrated that occupancy of NBD2 by ATP or ADP markedly decreased substrate binding by MRP1. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 98-102 15799713-1 2005 The Pgp (P-glycoprotein) multidrug transporter, which is linked to multidrug resistance in human cancers, functions as an efflux pump for non-polar drugs, powered by the hydrolysis of ATP at its nucleotide binding domains. Adenosine Triphosphate 184-187 ATP binding cassette subfamily B member 1 Homo sapiens 4-7 15799713-1 2005 The Pgp (P-glycoprotein) multidrug transporter, which is linked to multidrug resistance in human cancers, functions as an efflux pump for non-polar drugs, powered by the hydrolysis of ATP at its nucleotide binding domains. Adenosine Triphosphate 184-187 ATP binding cassette subfamily B member 1 Homo sapiens 9-23 15799713-4 2005 Here we use a dithionite fluorescence quenching technique to show that reconstituted Pgp can flip several NBD (nitrobenzo-2-oxa-1,3-diazole)-labelled simple glycosphingolipids, including NBD-GlcCer, from one leaflet of the bilayer to the other in an ATP-dependent, vanadate-sensitive fashion. Adenosine Triphosphate 250-253 ATP binding cassette subfamily B member 1 Homo sapiens 85-88 15755910-6 2005 We have further explored the relationship between nucleotide and substrate binding by examining the effects of various ATP analogs and ADP trapping, as well as mutations in conserved functional elements in the NBDs, on the ability of MRP1 to bind the photoactivatable, high-affinity substrate cysteinyl leukotriene C(4) (LTC(4))(.) Adenosine Triphosphate 119-122 ATP binding cassette subfamily B member 1 Homo sapiens 234-238 15900513-1 2005 Digoxin is a drug with a narrow therapeutic index, which is a substrate of the ATP-dependent efflux pump P-glycoprotein. Adenosine Triphosphate 79-82 ATP binding cassette subfamily B member 1 Homo sapiens 105-119 15640379-7 2005 ATP-dependent uptake of [3H]vinblastine in vesicles was osmotically sensitive, suggesting intravesicular accumulation, and was inhibited by verapamil, an ABCB1 inhibitor. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 154-159 15641790-1 2005 One cause of multidrug resistance is the overexpression of P-glycoprotein, a 170 kDa plasma membrane ABC transporter, which functions as an ATP-driven efflux pump with broad specificity for hydrophobic drugs, peptides, and natural products. Adenosine Triphosphate 140-143 ATP binding cassette subfamily B member 1 Homo sapiens 59-73 15886424-1 2005 P-glycoprotein (P-gp), an ATP-dependent efflux pump, is a membrane protein encoded by MDR1 gene, which demonstrates functional polymorphism. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 15886424-1 2005 P-glycoprotein (P-gp), an ATP-dependent efflux pump, is a membrane protein encoded by MDR1 gene, which demonstrates functional polymorphism. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 15886424-1 2005 P-glycoprotein (P-gp), an ATP-dependent efflux pump, is a membrane protein encoded by MDR1 gene, which demonstrates functional polymorphism. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 86-90 15581596-2 2005 Following intracellular metabolism by cytochrome P450 (CYP) enzymes, drug metabolites are excreted into bile or urine via ATP-dependent multidrug resistance proteins (MDR1 and MRPs). Adenosine Triphosphate 122-125 ATP binding cassette subfamily B member 1 Homo sapiens 167-171 15355964-0 2004 Mutation of the aromatic amino acid interacting with adenine moiety of ATP to a polar residue alters the properties of multidrug resistance protein 1. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 119-149 15476675-1 2004 P-glycoprotein (Pgp, ABCB1) is an ATP-dependent drug efflux pump linked to development of multidrug resistance (MDR) in cancer cells. Adenosine Triphosphate 34-37 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 15476675-1 2004 P-glycoprotein (Pgp, ABCB1) is an ATP-dependent drug efflux pump linked to development of multidrug resistance (MDR) in cancer cells. Adenosine Triphosphate 34-37 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 15476675-1 2004 P-glycoprotein (Pgp, ABCB1) is an ATP-dependent drug efflux pump linked to development of multidrug resistance (MDR) in cancer cells. Adenosine Triphosphate 34-37 ATP binding cassette subfamily B member 1 Homo sapiens 21-26 15535131-1 2004 Penetrance of anti-retroviral drugs into the CNS depends partly on the activity of P-glycoprotein (P-gp), an ATP-dependent efflux pump involved in restricting entry of lipophilic drugs into the brain. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 83-97 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 143-165 ATP binding cassette subfamily B member 1 Homo sapiens 4-26 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 143-165 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 143-165 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 143-165 ATP binding cassette subfamily B member 1 Homo sapiens 64-68 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 167-170 ATP binding cassette subfamily B member 1 Homo sapiens 4-26 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 167-170 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 167-170 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 15217827-1 2004 The multidrug resistance-1 (MDR1) gene product, P-glycoprotein (P-gp), and the multidrug resistance-related proteins (MRPs) are members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene superfamily that regulates the trafficking of drugs, peptides, ions, and xenobiotics across cell membrane barriers. Adenosine Triphosphate 167-170 ATP binding cassette subfamily B member 1 Homo sapiens 64-68 15217827-2 2004 Three-dimensional modeling of human MDR1/P-gp indicates that these glycoproteins function as efficient, ATP-dependent gate-keepers, which scan the plasma membrane and its inner leaflet to flip lipophilic substrates to the outer membrane leaflet. Adenosine Triphosphate 104-107 ATP binding cassette subfamily B member 1 Homo sapiens 36-40 15217827-2 2004 Three-dimensional modeling of human MDR1/P-gp indicates that these glycoproteins function as efficient, ATP-dependent gate-keepers, which scan the plasma membrane and its inner leaflet to flip lipophilic substrates to the outer membrane leaflet. Adenosine Triphosphate 104-107 ATP binding cassette subfamily B member 1 Homo sapiens 41-45 15535131-1 2004 Penetrance of anti-retroviral drugs into the CNS depends partly on the activity of P-glycoprotein (P-gp), an ATP-dependent efflux pump involved in restricting entry of lipophilic drugs into the brain. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 99-103 15379547-1 2004 P-Glycoprotein (P-gp) is an ATP-dependent drug pump that transports a broad range of compounds out of the cell. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 15482646-8 2004 Cloning and expression of the nucleotide binding domain of the human MDR1 gene in Escherichia coli was established, and by using colorimetry to examine the activity of ATPase to hydrolyse ATP, the ATPase activity of target nucleotide binding domain protein was determined. Adenosine Triphosphate 168-171 ATP binding cassette subfamily B member 1 Homo sapiens 69-73 15379547-1 2004 P-Glycoprotein (P-gp) is an ATP-dependent drug pump that transports a broad range of compounds out of the cell. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 15131592-0 2004 Mutational analysis of P-glycoprotein: suppression of caspase activation in the absence of ATP-dependent drug efflux. Adenosine Triphosphate 91-94 ATP binding cassette subfamily B member 1 Homo sapiens 23-37 15358539-2 2004 We found that bioactive peptides including somatostatin and substance P inhibit ATP-dependent vincristine binding to P-glycoprotein-overexpressing K562/ADM membrane vesicles. Adenosine Triphosphate 80-83 ATP binding cassette subfamily B member 1 Homo sapiens 117-131 15358539-4 2004 The K562/ADM membrane vesicles showed an ATP-dependent, osmotically sensitive uptake of somatostatin and substance P, which was inhibited by valspodar, an inhibitor of P-glycoprotein. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 168-182 15517878-2 2004 The adenosine triphosphate (ATP) binding cassette (ABC) family of transport proteins, such as the 170 kDa P-glycoprotein (multidrug resistance gene-1; MDR-1) and the 190 kDa multidrug resistance-associated proteins (MRPs), are associated with multidrug resistance, including resistance to CDDP. Adenosine Triphosphate 4-26 ATP binding cassette subfamily B member 1 Homo sapiens 106-120 15517878-2 2004 The adenosine triphosphate (ATP) binding cassette (ABC) family of transport proteins, such as the 170 kDa P-glycoprotein (multidrug resistance gene-1; MDR-1) and the 190 kDa multidrug resistance-associated proteins (MRPs), are associated with multidrug resistance, including resistance to CDDP. Adenosine Triphosphate 4-26 ATP binding cassette subfamily B member 1 Homo sapiens 151-156 15517878-2 2004 The adenosine triphosphate (ATP) binding cassette (ABC) family of transport proteins, such as the 170 kDa P-glycoprotein (multidrug resistance gene-1; MDR-1) and the 190 kDa multidrug resistance-associated proteins (MRPs), are associated with multidrug resistance, including resistance to CDDP. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 106-120 15517878-2 2004 The adenosine triphosphate (ATP) binding cassette (ABC) family of transport proteins, such as the 170 kDa P-glycoprotein (multidrug resistance gene-1; MDR-1) and the 190 kDa multidrug resistance-associated proteins (MRPs), are associated with multidrug resistance, including resistance to CDDP. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 151-156 15131592-1 2004 P-glycoprotein (P-gp) can induce multidrug resistance (MDR) through the ATP-dependent efflux of chemotherapeutic agents. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 15131592-1 2004 P-glycoprotein (P-gp) can induce multidrug resistance (MDR) through the ATP-dependent efflux of chemotherapeutic agents. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 15131592-3 2004 To determine if this additional activity is functionally linked to ATP hydrolysis, we expressed wild-type and ATPase-mutant P-gp and showed that cells expressing mutant P-gp could not efflux chemotherapeutic drugs but remained relatively resistant to apoptosis. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 124-128 15131592-3 2004 To determine if this additional activity is functionally linked to ATP hydrolysis, we expressed wild-type and ATPase-mutant P-gp and showed that cells expressing mutant P-gp could not efflux chemotherapeutic drugs but remained relatively resistant to apoptosis. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 169-173 15322232-1 2004 P-glycoprotein, an ATP-driven drug export pump, is a critical, selective component of the blood-brain barrier responsible for the poor penetration of many therapeutic drugs. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 15260484-0 2004 Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1. Adenosine Triphosphate 158-161 ATP binding cassette subfamily B member 1 Homo sapiens 26-56 15192095-9 2004 Our data are reconciled with a recent atomic scale model of P-gp and are consistent with a tilting of TM6 in response to nucleotide binding and ATP hydrolysis. Adenosine Triphosphate 144-147 ATP binding cassette subfamily B member 1 Homo sapiens 60-64 15260484-0 2004 Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1. Adenosine Triphosphate 158-161 ATP binding cassette subfamily B member 1 Homo sapiens 58-62 15260484-11 2004 Kinetic and photolabeling studies revealed that mutation N590A not only decreased the affinity of MRP1 for cysteinyl leukotriene 4 (LTC(4)) but also substantially reduced the binding of ATP to nucleotide binding domain 1 (NBD1). Adenosine Triphosphate 186-189 ATP binding cassette subfamily B member 1 Homo sapiens 98-102 14979722-1 2004 Human P-glycoprotein (Pgp) is as an ATP-dependent efflux pump for a variety of chemotherapeutic drugs. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 6-20 15155846-1 2004 Human multidrug resistance protein 1 (MRP1) is a membrane ATP-binding cassette transporter that confers multidrug resistance to tumor cells by effluxing intracellular drugs in an ATP-dependent manner. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 6-36 15155846-1 2004 Human multidrug resistance protein 1 (MRP1) is a membrane ATP-binding cassette transporter that confers multidrug resistance to tumor cells by effluxing intracellular drugs in an ATP-dependent manner. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 38-42 15155846-13 2004 Our findings suggest that both signature sequences of MRP1 are involved in ATP hydrolysis and must be intact for the ATP hydrolysis and the transport by MRP1. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 54-58 15155846-13 2004 Our findings suggest that both signature sequences of MRP1 are involved in ATP hydrolysis and must be intact for the ATP hydrolysis and the transport by MRP1. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 153-157 15155846-13 2004 Our findings suggest that both signature sequences of MRP1 are involved in ATP hydrolysis and must be intact for the ATP hydrolysis and the transport by MRP1. Adenosine Triphosphate 117-120 ATP binding cassette subfamily B member 1 Homo sapiens 54-58 15155846-13 2004 Our findings suggest that both signature sequences of MRP1 are involved in ATP hydrolysis and must be intact for the ATP hydrolysis and the transport by MRP1. Adenosine Triphosphate 117-120 ATP binding cassette subfamily B member 1 Homo sapiens 153-157 15212152-1 2004 The multidrug resistance (MDR1) gene product P-glycoprotein is a membrane protein that functions as an ATP-dependent efflux pump, transporting exogenous and endogenous substrates from the inside of cells to the outside. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 26-30 15212152-1 2004 The multidrug resistance (MDR1) gene product P-glycoprotein is a membrane protein that functions as an ATP-dependent efflux pump, transporting exogenous and endogenous substrates from the inside of cells to the outside. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 45-59 14999785-2 2004 The MDR1 gene product, Pgp, is an ATP-driven efflux pump, which extrudes a variety of dissimilar hydrophobic cytotoxic compounds from MDR cells. Adenosine Triphosphate 34-37 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 14985103-2 2004 The conformational changes of Pgp that occur in the presence of substrates/modulators or ATP depletion are accompanied by the up-shift of UIC2 monoclonal antibody (mAb) binding. Adenosine Triphosphate 89-92 ATP binding cassette subfamily B member 1 Homo sapiens 30-33 15003566-2 2004 Because MDR1 harnesses adenosine 5"-triphosphate (ATP) hydrolysis for transporting drugs, examining the effect on ATPase activity is imperative for understanding the interactions between drugs and MDR1. Adenosine Triphosphate 23-48 ATP binding cassette subfamily B member 1 Homo sapiens 8-12 15003566-2 2004 Because MDR1 harnesses adenosine 5"-triphosphate (ATP) hydrolysis for transporting drugs, examining the effect on ATPase activity is imperative for understanding the interactions between drugs and MDR1. Adenosine Triphosphate 23-48 ATP binding cassette subfamily B member 1 Homo sapiens 197-201 15003566-2 2004 Because MDR1 harnesses adenosine 5"-triphosphate (ATP) hydrolysis for transporting drugs, examining the effect on ATPase activity is imperative for understanding the interactions between drugs and MDR1. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 8-12 14979722-1 2004 Human P-glycoprotein (Pgp) is as an ATP-dependent efflux pump for a variety of chemotherapeutic drugs. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 22-25 14979722-6 2004 ATP hydrolysis by Pgp on the other hand is stimulated by the dimers with spacers of up to 22 A, whereas dimers with longer spacers inhibit ATP hydrolysis. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 18-21 14979722-6 2004 ATP hydrolysis by Pgp on the other hand is stimulated by the dimers with spacers of up to 22 A, whereas dimers with longer spacers inhibit ATP hydrolysis. Adenosine Triphosphate 139-142 ATP binding cassette subfamily B member 1 Homo sapiens 18-21 15022711-6 2004 Its function can be studied in vitro with different cell lines expressing P-glycoprotein in experiments using methods and equipment such as flow cytometry, cell proliferation, cell-free ATP as activity determination and Transwell culture equipment. Adenosine Triphosphate 186-189 ATP binding cassette subfamily B member 1 Homo sapiens 74-88 14965247-0 2004 ATP hydrolysis-dependent multidrug efflux transporter: MDR1/P-glycoprotein. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 55-59 14530869-1 2004 PURPOSE: One of the mechanisms responsible for the multidrug resistance (MDR) phenotype of cancer cells is overexpression of so-called ATP-dependent drug efflux proteins: the 170-kDa P-glycoprotein (P-gp) encoded by the MDR1 gene and the 190-kDa multidrug resistance-associated protein 1 encoded by the MRP1 gene. Adenosine Triphosphate 135-138 ATP binding cassette subfamily B member 1 Homo sapiens 183-197 14530869-1 2004 PURPOSE: One of the mechanisms responsible for the multidrug resistance (MDR) phenotype of cancer cells is overexpression of so-called ATP-dependent drug efflux proteins: the 170-kDa P-glycoprotein (P-gp) encoded by the MDR1 gene and the 190-kDa multidrug resistance-associated protein 1 encoded by the MRP1 gene. Adenosine Triphosphate 135-138 ATP binding cassette subfamily B member 1 Homo sapiens 199-203 14530869-1 2004 PURPOSE: One of the mechanisms responsible for the multidrug resistance (MDR) phenotype of cancer cells is overexpression of so-called ATP-dependent drug efflux proteins: the 170-kDa P-glycoprotein (P-gp) encoded by the MDR1 gene and the 190-kDa multidrug resistance-associated protein 1 encoded by the MRP1 gene. Adenosine Triphosphate 135-138 ATP binding cassette subfamily B member 1 Homo sapiens 220-224 14965247-10 2004 Visualization of the structure, as well as the biochemical data, is needed to fully understand how MDR1/P-glycoprotein recognizes such a variety of compounds and how it carries its substrates across the membrane using the energy from ATP hydrolysis. Adenosine Triphosphate 234-237 ATP binding cassette subfamily B member 1 Homo sapiens 104-118 14965248-1 2004 The human multidrug resistance gene (MDR1), spanning greater than 200 kb, encodes for the ATP-dependent membrane efflux transporter, P-glycoprotein (Pgp). Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 37-41 14965248-1 2004 The human multidrug resistance gene (MDR1), spanning greater than 200 kb, encodes for the ATP-dependent membrane efflux transporter, P-glycoprotein (Pgp). Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 133-147 14965248-1 2004 The human multidrug resistance gene (MDR1), spanning greater than 200 kb, encodes for the ATP-dependent membrane efflux transporter, P-glycoprotein (Pgp). Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 149-152 14744937-1 2004 P-glycoprotein (P-gp) is an ATP-dependent efflux membrane transporter involved in many drug pharmacokinetics in humans. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 14744937-1 2004 P-glycoprotein (P-gp) is an ATP-dependent efflux membrane transporter involved in many drug pharmacokinetics in humans. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 15072439-7 2004 Many of these herbal constituents, in particular flavonoids, were reported to modulate Pgp by directly interacting with the vicinal ATP-binding site, the steroid-binding site, or the substrate-binding site. Adenosine Triphosphate 132-135 ATP binding cassette subfamily B member 1 Homo sapiens 87-90 14965247-0 2004 ATP hydrolysis-dependent multidrug efflux transporter: MDR1/P-glycoprotein. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 14965247-6 2004 ATP binding and hydrolysis were extensively analyzed with the purified MDR1/P-glycoprotein. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 71-75 14965247-6 2004 ATP binding and hydrolysis were extensively analyzed with the purified MDR1/P-glycoprotein. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 76-90 14965247-7 2004 The vanadate-induced nucleotide trapping method was also applied to study the hydrolysis of ATP by MDR1/P-glycoprotein. Adenosine Triphosphate 92-95 ATP binding cassette subfamily B member 1 Homo sapiens 99-103 14965247-7 2004 The vanadate-induced nucleotide trapping method was also applied to study the hydrolysis of ATP by MDR1/P-glycoprotein. Adenosine Triphosphate 92-95 ATP binding cassette subfamily B member 1 Homo sapiens 104-118 14965247-8 2004 When MDR1 hydrolyzes ATP in the presence of excess orthovanadate, an analog of inorganic phosphate, it forms a metastable complex after hydrolysis. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 5-9 14965247-9 2004 Using this method, MDR1/P-glycoprotein can be specifically photoaffinity-labeled in the membrane, if 8-azido-[alpha(32)P]ATP is used as ATP. Adenosine Triphosphate 121-124 ATP binding cassette subfamily B member 1 Homo sapiens 19-23 14965247-9 2004 Using this method, MDR1/P-glycoprotein can be specifically photoaffinity-labeled in the membrane, if 8-azido-[alpha(32)P]ATP is used as ATP. Adenosine Triphosphate 121-124 ATP binding cassette subfamily B member 1 Homo sapiens 24-38 14965247-10 2004 Visualization of the structure, as well as the biochemical data, is needed to fully understand how MDR1/P-glycoprotein recognizes such a variety of compounds and how it carries its substrates across the membrane using the energy from ATP hydrolysis. Adenosine Triphosphate 234-237 ATP binding cassette subfamily B member 1 Homo sapiens 99-103 15630255-2 2004 In normal tissues, P-gp functions as an ATP-dependent efflux pump that excretes highly hydrophobic xenobiotic compounds, playing an important role in protecting the cells/tissues from xenobiotics. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 19-23 12968025-2 2003 Although it is well-established that Pgp exhibits drug-dependent ATPase and ATP-dependent drug transport functions, the mechanism by which these two reactions are coupled remains unclear. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 37-40 16305369-1 2004 In cancer and AIDS, overexpression of the MDR1 gene has important implications in the design of chemotherapy protocols because of the ability of its product, the ATP-dependent drug efflux pump P-glycoprotein (Pgp), to confer selective advantage to tumor and HIV-infected cells in the form of multidrug resistance. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 42-46 16305369-1 2004 In cancer and AIDS, overexpression of the MDR1 gene has important implications in the design of chemotherapy protocols because of the ability of its product, the ATP-dependent drug efflux pump P-glycoprotein (Pgp), to confer selective advantage to tumor and HIV-infected cells in the form of multidrug resistance. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 193-207 16305369-1 2004 In cancer and AIDS, overexpression of the MDR1 gene has important implications in the design of chemotherapy protocols because of the ability of its product, the ATP-dependent drug efflux pump P-glycoprotein (Pgp), to confer selective advantage to tumor and HIV-infected cells in the form of multidrug resistance. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 209-212 12968025-7 2003 We show that vanadate stably traps Pgp under two different conditions, one in the presence of ATP alone and the other in the presence of ATP and drug, suggesting the existence of two Pgp conformations. Adenosine Triphosphate 94-97 ATP binding cassette subfamily B member 1 Homo sapiens 35-38 12968025-7 2003 We show that vanadate stably traps Pgp under two different conditions, one in the presence of ATP alone and the other in the presence of ATP and drug, suggesting the existence of two Pgp conformations. Adenosine Triphosphate 137-140 ATP binding cassette subfamily B member 1 Homo sapiens 35-38 12968025-10 2003 These results together suggest that Pgp assumes at least two distinct conformational states, which catalyze two ATP hydrolysis events in the drug transport cycle, and the linker region mediates the transition between these two states of Pgp. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 36-39 12968025-10 2003 These results together suggest that Pgp assumes at least two distinct conformational states, which catalyze two ATP hydrolysis events in the drug transport cycle, and the linker region mediates the transition between these two states of Pgp. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 237-240 14586208-2 2003 MDR may be associated with the expression of the multidrug transporter glycoprotein p170, encoded by the MDR1 gene, which acts as an ATP-dependent efflux pump by reducing the intracellular accumulation of some cytotoxic agents. Adenosine Triphosphate 133-136 ATP binding cassette subfamily B member 1 Homo sapiens 105-109 14576852-8 2003 We have proposed a model for how ATP energizes transfer of substrates from these binding sites on P-gp to the outside of the cell, which accounts for the apparent stoichiometry of two ATPs hydrolysed per molecule of drug transported. Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 98-102 14598390-7 2003 Here I review studies demonstrating a major role for the ATP-driven, xenobiotic export pump, p-glycoprotein, in barrier function and recent experiments showing that transient inhibition of pump function can have substantial benefit for chemotherapy in an animal model of brain cancer. Adenosine Triphosphate 57-60 ATP binding cassette subfamily B member 1 Homo sapiens 93-107 14576852-8 2003 We have proposed a model for how ATP energizes transfer of substrates from these binding sites on P-gp to the outside of the cell, which accounts for the apparent stoichiometry of two ATPs hydrolysed per molecule of drug transported. Adenosine Triphosphate 184-188 ATP binding cassette subfamily B member 1 Homo sapiens 98-102 12932639-4 2003 The ATP depletion phenomenon was further examined using a panel of cells with varying levels of expression of P-glycoprotein (Pgp) and multidrug resistance-associated proteins (MRPs). Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 110-124 12932639-4 2003 The ATP depletion phenomenon was further examined using a panel of cells with varying levels of expression of P-glycoprotein (Pgp) and multidrug resistance-associated proteins (MRPs). Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 126-129 12731862-2 2003 MRP1 overexpression in tumor cells results in an ATP-dependent efflux of many oncolytic agents and arsenic and antimony oxyanions. Adenosine Triphosphate 49-52 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 12820887-11 2003 The results imply that the accessibility of residue 508, located in the alpha-helical subdomain of NBD1 in Pgp, is altered by the conformational changes that occur during ATP hydrolysis. Adenosine Triphosphate 171-174 ATP binding cassette subfamily B member 1 Homo sapiens 107-110 21709754-1 2003 P-glycoprotein (P-gp), the human multidrug resistant (MDR1) gene product and cancer multidrug resistance-associated adenosine triphosphate (ATP)-binding cassette (ABC) transporter, is physiologically expressed on peripheral blood mononuclear cells, but its role in cellular immunity is only beginning to be elucidated. Adenosine Triphosphate 116-138 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 21709754-1 2003 P-glycoprotein (P-gp), the human multidrug resistant (MDR1) gene product and cancer multidrug resistance-associated adenosine triphosphate (ATP)-binding cassette (ABC) transporter, is physiologically expressed on peripheral blood mononuclear cells, but its role in cellular immunity is only beginning to be elucidated. Adenosine Triphosphate 116-138 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 21709754-1 2003 P-glycoprotein (P-gp), the human multidrug resistant (MDR1) gene product and cancer multidrug resistance-associated adenosine triphosphate (ATP)-binding cassette (ABC) transporter, is physiologically expressed on peripheral blood mononuclear cells, but its role in cellular immunity is only beginning to be elucidated. Adenosine Triphosphate 140-143 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 12843573-6 2003 The ATP binding cassette transporter B1 (ABCB1) mediates the efflux transport of lipophilic drugs at the BBB by using ATP energy. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 41-46 12894516-0 2003 Modulation by the ATP/GTP ratio of the phosphorylation level of P-glycoprotein and of various plasma membrane proteins of KB-V1 multidrug resistant cells. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 64-78 12549939-1 2003 Drug transport mediated by P-glycoprotein (Pgp) is driven by hydrolysis of ATP at the two cytosolic nucleotide binding domains. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 27-41 12551908-2 2003 Pdr5p shares similar mechanisms of substrate recognition and transport with the human MDR1-Pgp, despite an inverted topology of transmembrane and ATP-binding domains. Adenosine Triphosphate 146-149 ATP binding cassette subfamily B member 1 Homo sapiens 86-90 12549939-1 2003 Drug transport mediated by P-glycoprotein (Pgp) is driven by hydrolysis of ATP at the two cytosolic nucleotide binding domains. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 43-46 12549939-5 2003 Both resting and transition state P-glycoprotein showed similar affinity for TNP-ATP/TNP-ADP and unlabeled ATP/ADP. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 34-48 12549939-8 2003 These results indicate that both nucleotide binding domains of P-glycoprotein are likely to be occupied with either ATP (or ADP) in the resting state and the transition state in the absence of transport substrates. Adenosine Triphosphate 116-119 ATP binding cassette subfamily B member 1 Homo sapiens 63-77 12549939-9 2003 Drugs alter the binding affinity to favor association of ATP with P-glycoprotein at the start of the catalytic cycle and release of ADP from the transition state following nucleotide hydrolysis. Adenosine Triphosphate 57-60 ATP binding cassette subfamily B member 1 Homo sapiens 66-80 12421806-1 2003 The human multidrug resistance P-glycoprotein (P-gp, ABCB1) uses ATP to transport many structurally diverse compounds out of the cell. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 31-45 12421806-1 2003 The human multidrug resistance P-glycoprotein (P-gp, ABCB1) uses ATP to transport many structurally diverse compounds out of the cell. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 53-58 12359865-1 2003 P-glycoprotein is an ATP-dependent efflux pump that contributes to the protection of the body from environmental toxins. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 12466237-2 2002 Increasing the lipophilicity is a strategy often used to improve a compound"s cellular uptake and retention but this may also convert it into a substrate for an ATP-dependent transporter such as P-glycoprotein or the multidrug resistance-associated protein (MRP1), which are involved in cellular efflux of drugs. Adenosine Triphosphate 161-164 ATP binding cassette subfamily B member 1 Homo sapiens 195-209 12608534-9 2003 In the presence of a Pgp inhibitor such as verapamil, cyclosporine A, or progesterone, the ATP-dependent uptakes of [3H]digoxin and [3H]vinblastine into BBMVs were significantly reduced. Adenosine Triphosphate 91-94 ATP binding cassette subfamily B member 1 Homo sapiens 21-24 12486090-1 2002 P-Glycoprotein (P-gp) is an ATP-dependent efflux transporter that extrudes non-polar molecules, including cytotoxic substances and drugs, from the cells. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 12486090-1 2002 P-Glycoprotein (P-gp) is an ATP-dependent efflux transporter that extrudes non-polar molecules, including cytotoxic substances and drugs, from the cells. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 12437356-0 2002 Importance of the conserved Walker B glutamate residues, 556 and 1201, for the completion of the catalytic cycle of ATP hydrolysis by human P-glycoprotein (ABCB1). Adenosine Triphosphate 116-119 ATP binding cassette subfamily B member 1 Homo sapiens 140-154 12244102-1 2002 ATP-driven pumping of a variety of drugs out of cells by the human P-glycoprotein poses a serious problem to medical therapy. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 12406646-1 2002 The MDR1 (ABCB1) gene product P-glycoprotein is a membrane protein, which functions as an ATP-dependent exporter of xenobiotics from cells. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 12406646-1 2002 The MDR1 (ABCB1) gene product P-glycoprotein is a membrane protein, which functions as an ATP-dependent exporter of xenobiotics from cells. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 10-15 12406646-1 2002 The MDR1 (ABCB1) gene product P-glycoprotein is a membrane protein, which functions as an ATP-dependent exporter of xenobiotics from cells. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 30-44 12437356-0 2002 Importance of the conserved Walker B glutamate residues, 556 and 1201, for the completion of the catalytic cycle of ATP hydrolysis by human P-glycoprotein (ABCB1). Adenosine Triphosphate 116-119 ATP binding cassette subfamily B member 1 Homo sapiens 156-161 12437356-1 2002 The human MDR1 (ABCB1) gene product, P-glycoprotein (Pgp), functions as an ATP-dependent efflux pump for a variety of chemotherapeutic drugs. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 10-14 12437356-1 2002 The human MDR1 (ABCB1) gene product, P-glycoprotein (Pgp), functions as an ATP-dependent efflux pump for a variety of chemotherapeutic drugs. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 16-21 12437356-1 2002 The human MDR1 (ABCB1) gene product, P-glycoprotein (Pgp), functions as an ATP-dependent efflux pump for a variety of chemotherapeutic drugs. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 37-51 12437356-1 2002 The human MDR1 (ABCB1) gene product, P-glycoprotein (Pgp), functions as an ATP-dependent efflux pump for a variety of chemotherapeutic drugs. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 53-56 12437356-2 2002 In this study, we assessed the role of conserved glutamate residues in the Walker B domain of the two ATP sites (E556 and E1201, respectively) during the catalytic cycle of human Pgp. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 179-182 12437356-4 2002 Although steady-state ATP hydrolysis and drug transport activities were abrogated in both E556Q and E1201Q mutant Pgps, [alpha-(32)P]-8-azidoADP was trapped in the presence of vanadate (Vi), and the release of trapped [alpha-(32)P]-8-azidoADP occurred to a similar extent as in wild-type Pgp. Adenosine Triphosphate 22-25 ATP binding cassette subfamily B member 1 Homo sapiens 114-117 12210557-1 2002 P-glycoprotein (P-gp) is a plasma membrane glycoprotein that confers multidrug resistance on cells by virtue of its ability to exclude cytotoxic drugs in an ATP-dependent manner. Adenosine Triphosphate 157-160 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 12138119-1 2002 Multidrug resistance protein 1 (MRP1/ABCC1) is an ATP-dependent transporter of structurally diverse organic anion conjugates. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 0-30 12210557-1 2002 P-glycoprotein (P-gp) is a plasma membrane glycoprotein that confers multidrug resistance on cells by virtue of its ability to exclude cytotoxic drugs in an ATP-dependent manner. Adenosine Triphosphate 157-160 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 12210557-2 2002 The most commonly considered hypothesis is that P-gp acts as an ATP-driven drug-export pump, the mechanism of which is not understood in detail. Adenosine Triphosphate 64-67 ATP binding cassette subfamily B member 1 Homo sapiens 48-52 12034727-11 2002 Finally, vanadate-induced trapping of ADP inhibited [125I]LY475776 labeling, suggesting that ATP hydrolysis causes a conformational change in MRP1 that reduces the affinity of the protein for this inhibitor. Adenosine Triphosphate 93-96 ATP binding cassette subfamily B member 1 Homo sapiens 142-146 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 32-35 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 97-100 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 32-35 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 97-100 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 32-35 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 97-100 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 32-35 12163030-3 2002 We found that: (1) the NBD1s of Pgp and MRP1 have ATPase and GTPase activities, (2) the K(m)s of Pgp NBD1 for ATP and GTP hydrolysis are identical, while the K(m) of MRP1 NBD1 for ATP is lower than that for GTP, and (3) phosphorylation of MLD by PKA or PKC produces a marginal increase of V(max) for ATP hydrolysis, without affecting the affinity for ATP. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 97-100 12145328-2 2002 P-gp catalyzes the ATP hydrolysis-dependent efflux of numerous amphiphilic compounds of unrelated chemical structures. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 12047375-0 2002 Effects of ATP depletion and phosphate analogues on P-glycoprotein conformation in live cells. Adenosine Triphosphate 11-14 ATP binding cassette subfamily B member 1 Homo sapiens 52-66 12047375-1 2002 P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. Adenosine Triphosphate 185-188 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 12047375-1 2002 P-glycoprotein (Pgp), a membrane pump often responsible for the multidrug resistance of cancer cells, undergoes conformational changes in the presence of substrates/modulators, or upon ATP depletion, reflected by its enhanced reactivity with the UIC2 monoclonal antibody. Adenosine Triphosphate 185-188 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 12021398-3 2002 The drug extrusion is ATP-dependent and can be inhibited by chemosensitizers, such as the dihydropyridine derivative dexniguldipine-HCl, through direct interaction with P-gp. Adenosine Triphosphate 22-25 ATP binding cassette subfamily B member 1 Homo sapiens 169-173 11979447-1 2002 Multidrug resistance (MDR) mediated by the ATP-dependent efflux protein P-glycoprotein (P-gp) is a major obstacle to the successful treatment of many cancers. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 72-86 12031686-1 2002 The transmembrane transporter P-glycoprotein (P-gp) is an ATP-dependent efflux pump for a wide range of drugs. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 30-44 12031686-1 2002 The transmembrane transporter P-glycoprotein (P-gp) is an ATP-dependent efflux pump for a wide range of drugs. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 46-50 11979447-1 2002 Multidrug resistance (MDR) mediated by the ATP-dependent efflux protein P-glycoprotein (P-gp) is a major obstacle to the successful treatment of many cancers. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 88-92 11990782-1 2002 The human P glycoprotein (Pgp; MDR1) is an ATP-driven transporter for hydrophobic drugs and causes multidrug resistance in cancer. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 10-24 11926837-1 2002 The P-glycoprotein multidrug transporter carries out ATP-driven cellular efflux of a wide variety of hydrophobic drugs, natural products, and peptides. Adenosine Triphosphate 53-56 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 11915946-2 2002 P-glycoprotein (ABCB1, according to the human gene nomenclature committee) consists of two homologous halves each containing a transmembrane domain (TMD) involved in drug binding and efflux, and a cytosolic nucleotide-binding domain (NBD) involved in ATP binding and hydrolysis, with an overall (TMD-NBD)2 domain topology. Adenosine Triphosphate 251-254 ATP binding cassette subfamily B member 1 Homo sapiens 16-21 11751127-9 2002 The effects of these azole antifungals on ATP consumption by P-gp (representing transport activity) were also assessed, and the K(m) values were congruent with the IC(50)s. Therefore, exposure of tissue to the azole antifungals may be modulated by human P-gp, and the clinical interactions of azole antifungals with other drugs may be due, in part, to inhibition of P-gp transport. Adenosine Triphosphate 42-45 ATP binding cassette subfamily B member 1 Homo sapiens 61-65 11934808-8 2002 We observed ATP-dependent uptake of all drugs studied into MDR1- and Mdr1b-expressing vesicles. Adenosine Triphosphate 12-15 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 11990782-1 2002 The human P glycoprotein (Pgp; MDR1) is an ATP-driven transporter for hydrophobic drugs and causes multidrug resistance in cancer. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 26-29 11990782-1 2002 The human P glycoprotein (Pgp; MDR1) is an ATP-driven transporter for hydrophobic drugs and causes multidrug resistance in cancer. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 31-35 11990782-2 2002 Our knowledge related to the mechanistic details of the ATP hydrolytic cycle of MDR1 has recently significantly progressed due to studies on the formation of a catalytic intermediate (occluded nucleotide state). Adenosine Triphosphate 56-59 ATP binding cassette subfamily B member 1 Homo sapiens 80-84 11990782-3 2002 According to the most accepted current model, both catalytic sites in MDR1 are active and ATP is hydrolysed alternatively within the two sites. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 70-74 11898391-2 2002 The photolabile conjugated triene structure of LTC4 has enabled direct photoaffinity labeling of the multidrug resistance protein 1 (MRP1, symbol ABC C1) in membranes from mastocytoma cells, leading to the identification of the function of this protein as an ATP-dependent export pump for LTC4 and structurally related conjugates. Adenosine Triphosphate 259-262 ATP binding cassette subfamily B member 1 Homo sapiens 101-131 11898391-2 2002 The photolabile conjugated triene structure of LTC4 has enabled direct photoaffinity labeling of the multidrug resistance protein 1 (MRP1, symbol ABC C1) in membranes from mastocytoma cells, leading to the identification of the function of this protein as an ATP-dependent export pump for LTC4 and structurally related conjugates. Adenosine Triphosphate 259-262 ATP binding cassette subfamily B member 1 Homo sapiens 133-137 11745490-2 2001 Pgp is an ATP-driven efflux pump that extrudes a variety of dissimilar hydrophobic cytotoxic compounds. Adenosine Triphosphate 10-13 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 11803416-1 2001 P-glycoprotein (Pgp), an ATP-dependent plasma membrane efflux pump, is expressed in abundance on the luminal aspect of brain capillary endothelial cells and astrocytes of the blood-brain barrier where it limits the passage of a variety of lipophilic substrates into the central nervous system. Adenosine Triphosphate 25-28 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11804190-1 2001 P-glycoprotein (Pgp), the ATP-binding cassette (ABC) transporter, confers multidrug resistance to cancer cells by extruding cytotoxic natural product amphipathic drugs using the energy of ATP hydrolysis. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11804190-1 2001 P-glycoprotein (Pgp), the ATP-binding cassette (ABC) transporter, confers multidrug resistance to cancer cells by extruding cytotoxic natural product amphipathic drugs using the energy of ATP hydrolysis. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 11804190-2 2001 Our studies are directed toward understanding the mechanism of action of Pgp and recent work deals with the assessment of interaction between substrate and ATP sites and elucidation of the catalytic cycle of ATP hydrolysis. Adenosine Triphosphate 156-159 ATP binding cassette subfamily B member 1 Homo sapiens 73-76 11804190-2 2001 Our studies are directed toward understanding the mechanism of action of Pgp and recent work deals with the assessment of interaction between substrate and ATP sites and elucidation of the catalytic cycle of ATP hydrolysis. Adenosine Triphosphate 208-211 ATP binding cassette subfamily B member 1 Homo sapiens 73-76 11804190-3 2001 The kinetic analyses of ATP hydrolysis by reconstituted purified Pgp suggest that ADP release is the rate-limiting step in the catalytic cycle and the substrates exert their effect by modulating ADP release. Adenosine Triphosphate 24-27 ATP binding cassette subfamily B member 1 Homo sapiens 65-68 11804190-4 2001 In addition, we provide evidence for two distinct roles for ATP hydrolysis in a single turnover of Pgp, one in the transport of drug and the other in effecting conformational changes so as to reset the transporter for the next catalytic cycle. Adenosine Triphosphate 60-63 ATP binding cassette subfamily B member 1 Homo sapiens 99-102 11804190-7 2001 Thus, the blocking of ATP-binding to the second site while the first one is in catalytic conformation appears to be the basis for the alternate catalytic cycle of ATP hydrolysis by Pgp, and this may be applicable as well to other ABC transporters linked with the development of multidrug resistance. Adenosine Triphosphate 22-25 ATP binding cassette subfamily B member 1 Homo sapiens 181-184 11804190-7 2001 Thus, the blocking of ATP-binding to the second site while the first one is in catalytic conformation appears to be the basis for the alternate catalytic cycle of ATP hydrolysis by Pgp, and this may be applicable as well to other ABC transporters linked with the development of multidrug resistance. Adenosine Triphosphate 163-166 ATP binding cassette subfamily B member 1 Homo sapiens 181-184 11803416-1 2001 P-glycoprotein (Pgp), an ATP-dependent plasma membrane efflux pump, is expressed in abundance on the luminal aspect of brain capillary endothelial cells and astrocytes of the blood-brain barrier where it limits the passage of a variety of lipophilic substrates into the central nervous system. Adenosine Triphosphate 25-28 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 11602658-8 2001 A combination of experiments examining the kinetics, concentration dependence, and directionality of P85 effects on Pgp-mediated efflux in BBMEC monolayers suggests that both energy depletion (decreasing ATP pool available for Pgp) and membrane fluidization (inhibiting Pgp ATPase activity) are critical factors contributing to the activity of the block copolymer in the BBB. Adenosine Triphosphate 204-207 ATP binding cassette subfamily B member 1 Homo sapiens 116-119 11684287-2 2001 One of the most common and well-studied mechanisms implicated in causing MDR is P-glycoprotein (Pgp), an ATP-dependent, transmembrane drug efflux pump. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 80-94 11684287-2 2001 One of the most common and well-studied mechanisms implicated in causing MDR is P-glycoprotein (Pgp), an ATP-dependent, transmembrane drug efflux pump. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 96-99 11602658-8 2001 A combination of experiments examining the kinetics, concentration dependence, and directionality of P85 effects on Pgp-mediated efflux in BBMEC monolayers suggests that both energy depletion (decreasing ATP pool available for Pgp) and membrane fluidization (inhibiting Pgp ATPase activity) are critical factors contributing to the activity of the block copolymer in the BBB. Adenosine Triphosphate 204-207 ATP binding cassette subfamily B member 1 Homo sapiens 227-230 11745042-2 2001 The recently described adenosine triphosphate (ATP)-dependent bile acid transporter, bile salt export pump (BSEP), formerly called sister of p-glycoprotein, is responsible for active transport of bile acids across the hepatocyte canalicular membrane into bile. Adenosine Triphosphate 23-45 ATP binding cassette subfamily B member 1 Homo sapiens 141-155 11745042-2 2001 The recently described adenosine triphosphate (ATP)-dependent bile acid transporter, bile salt export pump (BSEP), formerly called sister of p-glycoprotein, is responsible for active transport of bile acids across the hepatocyte canalicular membrane into bile. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 141-155 11451943-0 2001 Evidence for the vectorial nature of drug (substrate)-stimulated ATP hydrolysis by human P-glycoprotein. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 89-103 11507101-6 2001 These data suggest that MRP1-mediated transport involves a conformational change, driven by ATP hydrolysis at NBD2, that alters the affinity with which LTC(4) binds to one of two sites composed, at least in part, of elements in the NH(2)-proximal half of the protein. Adenosine Triphosphate 92-95 ATP binding cassette subfamily B member 1 Homo sapiens 24-28 11598005-1 2001 P-glycoprotein (P-gp) is an ABC (ATP-binding cassette) transporter, which hydrolyses ATP and extrudes cytotoxic drugs from mammalian cells. Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11598005-1 2001 P-glycoprotein (P-gp) is an ABC (ATP-binding cassette) transporter, which hydrolyses ATP and extrudes cytotoxic drugs from mammalian cells. Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 11451943-7 2001 Our results demonstrate that during the catalytic cycle of Pgp, although the transition state, Pgp x ADP x P(i) (Vi), can be generated both via the hydrolysis of ATP or by directly providing ADP to the system, in the presence of substrate the reaction is driven in the forward direction, i.e. hydrolysis of ATP. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 59-62 11451943-7 2001 Our results demonstrate that during the catalytic cycle of Pgp, although the transition state, Pgp x ADP x P(i) (Vi), can be generated both via the hydrolysis of ATP or by directly providing ADP to the system, in the presence of substrate the reaction is driven in the forward direction, i.e. hydrolysis of ATP. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 95-98 11451943-7 2001 Our results demonstrate that during the catalytic cycle of Pgp, although the transition state, Pgp x ADP x P(i) (Vi), can be generated both via the hydrolysis of ATP or by directly providing ADP to the system, in the presence of substrate the reaction is driven in the forward direction, i.e. hydrolysis of ATP. Adenosine Triphosphate 307-310 ATP binding cassette subfamily B member 1 Homo sapiens 59-62 11451943-7 2001 Our results demonstrate that during the catalytic cycle of Pgp, although the transition state, Pgp x ADP x P(i) (Vi), can be generated both via the hydrolysis of ATP or by directly providing ADP to the system, in the presence of substrate the reaction is driven in the forward direction, i.e. hydrolysis of ATP. Adenosine Triphosphate 307-310 ATP binding cassette subfamily B member 1 Homo sapiens 95-98 11451943-8 2001 These data suggest that substrate-stimulated ATP hydrolysis by Pgp is a vectorial process. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 63-66 11427347-8 2001 Participation of high-energy ATP molecule is required by P-glycoprotein (Pgp) and by MRP protein described in this paper for their action. Adenosine Triphosphate 29-32 ATP binding cassette subfamily B member 1 Homo sapiens 57-71 11454724-8 2001 ATP hydrolysis activity was measured in the membrane fraction prepared from MDR cells presenting P-gp, which were exposed to various concentrations of test compounds. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 11454724-9 2001 Known substrates of P-gp demonstrated clear, repeatable, concentration-dependent increases in ATP hydrolysis activity. Adenosine Triphosphate 94-97 ATP binding cassette subfamily B member 1 Homo sapiens 20-24 11429407-0 2001 Cross-linking of human multidrug resistance P-glycoprotein by the substrate, tris-(2-maleimidoethyl)amine, is altered by ATP hydrolysis. Adenosine Triphosphate 121-124 ATP binding cassette subfamily B member 1 Homo sapiens 44-58 11427347-8 2001 Participation of high-energy ATP molecule is required by P-glycoprotein (Pgp) and by MRP protein described in this paper for their action. Adenosine Triphosphate 29-32 ATP binding cassette subfamily B member 1 Homo sapiens 73-76 11278745-1 2001 P-glycoprotein (P-gp) is an ATP-dependent drug pump that confers multidrug resistance (MDR). Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11437348-4 2001 Using immunological approaches, the present studies were designed to elucidate the relative contributions of RLIP76, MRP1, and P-glycoprotein (Pgp), in the ATP-dependent transport of GS-E and DOX in human erythrocytes. Adenosine Triphosphate 156-159 ATP binding cassette subfamily B member 1 Homo sapiens 127-141 11437348-4 2001 Using immunological approaches, the present studies were designed to elucidate the relative contributions of RLIP76, MRP1, and P-glycoprotein (Pgp), in the ATP-dependent transport of GS-E and DOX in human erythrocytes. Adenosine Triphosphate 156-159 ATP binding cassette subfamily B member 1 Homo sapiens 143-146 11287418-1 2001 P-glycoprotein (Pgp) is an ATP-dependent drug efflux pump whose overexpression confers multidrug resistance to cancer cells. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11389609-1 2001 The P-glycoprotein multidrug transporter acts as an ATP-powered efflux pump for a large variety of hydrophobic drugs, natural products, and peptides. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 11428917-2 2001 This phenomenon is due in large part to the overexpression of a 170 kDa plasma membrane ATP-dependent pump known as the multidrug resistance transporter or P-glycoprotein. Adenosine Triphosphate 88-91 ATP binding cassette subfamily B member 1 Homo sapiens 156-170 11278745-1 2001 P-glycoprotein (P-gp) is an ATP-dependent drug pump that confers multidrug resistance (MDR). Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 11154703-9 2001 97, 2515-2520) that there are two independent ATP hydrolysis events in a single catalytic cycle of Pgp. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 99-102 11336637-0 2001 Role of glycine-534 and glycine-1179 of human multidrug resistance protein (MDR1) in drug-mediated control of ATP hydrolysis. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 76-80 11154703-16 2001 In aggregate, these findings provide an explanation for the alternate catalysis of ATP hydrolysis and offer a mechanistic framework to elucidate events at both the substrate- and nucleotide-binding sites in the catalytic cycle of Pgp. Adenosine Triphosphate 83-86 ATP binding cassette subfamily B member 1 Homo sapiens 230-233 11284688-9 2001 Colchicine increases the UIC2 reactivity and reverses the effect of ATP in ATPase-deficient Pgp mutants, but not in the wild-type Pgp expressed in the same cellular background, suggesting that ATP hydrolysis counteracts the effects of colchicine on the Pgp conformation. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 92-95 11154703-0 2001 Characterization of the catalytic cycle of ATP hydrolysis by human P-glycoprotein. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 11154703-2 2001 P-glycoprotein (Pgp) is a plasma membrane protein whose overexpression confers multidrug resistance to tumor cells by extruding amphipathic natural product cytotoxic drugs using the energy of ATP. Adenosine Triphosphate 192-195 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11154703-2 2001 P-glycoprotein (Pgp) is a plasma membrane protein whose overexpression confers multidrug resistance to tumor cells by extruding amphipathic natural product cytotoxic drugs using the energy of ATP. Adenosine Triphosphate 192-195 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 11284688-9 2001 Colchicine increases the UIC2 reactivity and reverses the effect of ATP in ATPase-deficient Pgp mutants, but not in the wild-type Pgp expressed in the same cellular background, suggesting that ATP hydrolysis counteracts the effects of colchicine on the Pgp conformation. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 92-95 11121420-0 2001 Correlation between steady-state ATP hydrolysis and vanadate-induced ADP trapping in Human P-glycoprotein. Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 91-105 11121420-2 2001 P-glycoprotein (Pgp) is a transmembrane protein conferring multidrug resistance to cells by extruding a variety of amphipathic cytotoxic agents using energy from ATP hydrolysis. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11121420-2 2001 P-glycoprotein (Pgp) is a transmembrane protein conferring multidrug resistance to cells by extruding a variety of amphipathic cytotoxic agents using energy from ATP hydrolysis. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 11121420-3 2001 The objective of this study was to understand how substrates affect the catalytic cycle of ATP hydrolysis by Pgp. Adenosine Triphosphate 91-94 ATP binding cassette subfamily B member 1 Homo sapiens 109-112 11121420-5 2001 Pgp hydrolyzes ATP in the absence of drug at a basal rate of 0.5 micromol x min x mg(-1) with a K(m) for ATP of 0.33 mm. Adenosine Triphosphate 15-18 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 11121420-5 2001 Pgp hydrolyzes ATP in the absence of drug at a basal rate of 0.5 micromol x min x mg(-1) with a K(m) for ATP of 0.33 mm. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 11121420-7 2001 Although inhibitors of Pgp activity, cyclosporin A, its analog PSC833, and rapamycin decrease the rate of ATP hydrolysis with respect to the basal rate, they do not completely inhibit the activity. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 23-26 11121420-14 2001 We suggest that substrates modulate the rate of ATPase activity of Pgp by controlling the rate of dissociation of ADP following ATP hydrolysis and that ADP release is the rate-limiting step in the normal catalytic cycle of Pgp. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 67-70 11121420-14 2001 We suggest that substrates modulate the rate of ATPase activity of Pgp by controlling the rate of dissociation of ADP following ATP hydrolysis and that ADP release is the rate-limiting step in the normal catalytic cycle of Pgp. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 223-226 11248673-1 2001 The multidrug resistant (MDR) tumor phenotype, characterized by a decreased cellular drug accumulation is achieved by ATP-dependent extrusions of drugs from cells by P-glycoprotein (P-gp) and/or by multidrug resistance protein (MRP1). Adenosine Triphosphate 118-121 ATP binding cassette subfamily B member 1 Homo sapiens 166-180 11248673-1 2001 The multidrug resistant (MDR) tumor phenotype, characterized by a decreased cellular drug accumulation is achieved by ATP-dependent extrusions of drugs from cells by P-glycoprotein (P-gp) and/or by multidrug resistance protein (MRP1). Adenosine Triphosphate 118-121 ATP binding cassette subfamily B member 1 Homo sapiens 182-186 11181832-6 2001 Finally, we were able to directly measure transport of Abeta peptides across the plasma membranes of p-gp enriched vesicles, and showed that this phenomenon was both ATP- and p-gp-dependent. Adenosine Triphosphate 166-169 ATP binding cassette subfamily B member 1 Homo sapiens 101-105 11284449-1 2001 Digoxin is a drug with a narrow therapeutic index, which is substrate of the ATP-dependent efflux pump P-glycoprotein. Adenosine Triphosphate 77-80 ATP binding cassette subfamily B member 1 Homo sapiens 103-117 11101309-1 2000 P-glycoprotein is a member of the ATP binding cassette family of membrane proteins, and acts as an ATP-driven efflux pump for a diverse group of hydrophobic drugs, natural products, and peptides. Adenosine Triphosphate 34-37 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11258197-1 2001 Transport by ATP-dependent efflux pumps, such as P-glycoprotein (PGP) and multi-drug resistance related proteins (MRPs), influences bioavailability and disposition of drugs. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 49-63 11258197-1 2001 Transport by ATP-dependent efflux pumps, such as P-glycoprotein (PGP) and multi-drug resistance related proteins (MRPs), influences bioavailability and disposition of drugs. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 65-68 11358379-0 2001 Cellular and biophysical evidence for interactions between adenosine triphosphate and P-glycoprotein substrates: functional implications for adenosine triphosphate/drug cotransport in P-glycoprotein overexpressing tumor cells and in P-glycoprotein low-level expressing erythrocytes. Adenosine Triphosphate 59-81 ATP binding cassette subfamily B member 1 Homo sapiens 86-100 11358379-0 2001 Cellular and biophysical evidence for interactions between adenosine triphosphate and P-glycoprotein substrates: functional implications for adenosine triphosphate/drug cotransport in P-glycoprotein overexpressing tumor cells and in P-glycoprotein low-level expressing erythrocytes. Adenosine Triphosphate 59-81 ATP binding cassette subfamily B member 1 Homo sapiens 184-198 11358379-0 2001 Cellular and biophysical evidence for interactions between adenosine triphosphate and P-glycoprotein substrates: functional implications for adenosine triphosphate/drug cotransport in P-glycoprotein overexpressing tumor cells and in P-glycoprotein low-level expressing erythrocytes. Adenosine Triphosphate 59-81 ATP binding cassette subfamily B member 1 Homo sapiens 184-198 11771758-1 2001 Transport by ATP-dependent efflux pumps such as P glycoprotein (Pgp) and multidrug resistance associated protein (MRP), encoded by multidrug resistant (MDR) associated genes, is an increasingly recognized mechanism by which cells maintain substrate homeostasis and evade drug therapy. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 11771758-1 2001 Transport by ATP-dependent efflux pumps such as P glycoprotein (Pgp) and multidrug resistance associated protein (MRP), encoded by multidrug resistant (MDR) associated genes, is an increasingly recognized mechanism by which cells maintain substrate homeostasis and evade drug therapy. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 64-67 11845298-4 2001 The wild-type mdr1 gene product, Pgp, enables the spontaneous release of cellular ATP. Adenosine Triphosphate 82-85 ATP binding cassette subfamily B member 1 Homo sapiens 14-18 11101309-7 2000 P-glycoprotein fluorescence was highly quenched on binding of fluorescent nucleotides, and moderately quenched by ATP, ADP, and AMP-PNP, suggesting that the site for nucleotide binding is located relatively close to tryptophan residues. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11101309-11 2000 Thus, the regions of P-glycoprotein involved in nucleotide and drug binding appear to be packed together compactly, which would facilitate coupling of ATP hydrolysis to drug transport. Adenosine Triphosphate 151-154 ATP binding cassette subfamily B member 1 Homo sapiens 21-35 11321524-2 2000 The main multidrug resistance (MDR) mechanism in tumour cells is the expression of P-gly-coprotein (P-gly), that acts as an ATP-dependent active efflux pump of chemotherapeutic agents. Adenosine Triphosphate 124-127 ATP binding cassette subfamily B member 1 Homo sapiens 83-98 11321524-2 2000 The main multidrug resistance (MDR) mechanism in tumour cells is the expression of P-gly-coprotein (P-gly), that acts as an ATP-dependent active efflux pump of chemotherapeutic agents. Adenosine Triphosphate 124-127 ATP binding cassette subfamily B member 1 Homo sapiens 83-88 11009602-0 2000 Drug binding sites on P-glycoprotein are altered by ATP binding prior to nucleotide hydrolysis. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 22-36 11027568-7 2000 Additionally, using the ATP-hydrolysis assay, we showed that cholesterol increases P-gp-mediated ATP hydrolysis by approximately 1.6-fold with a K(s) of 5 microM. Adenosine Triphosphate 24-27 ATP binding cassette subfamily B member 1 Homo sapiens 83-87 11027568-7 2000 Additionally, using the ATP-hydrolysis assay, we showed that cholesterol increases P-gp-mediated ATP hydrolysis by approximately 1.6-fold with a K(s) of 5 microM. Adenosine Triphosphate 97-100 ATP binding cassette subfamily B member 1 Homo sapiens 83-87 11009602-1 2000 P-glycoprotein (P-gp) confers multiple drug resistance on cancer cells by acting as a plasma membrane localized ATP-dependent drug efflux pump. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11009602-1 2000 P-glycoprotein (P-gp) confers multiple drug resistance on cancer cells by acting as a plasma membrane localized ATP-dependent drug efflux pump. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 11006111-1 2000 P-glycoprotein (P-gp) is an ATP-dependent drug pump that confers multidrug resistance. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 11498397-1 2000 The identification of certain members of the large superfamily of ATP binding cassette transport proteins such as MDR1 -P-glycoprotein and the multidrug resistance protein MRP1 as ATP-dependent drug efflux pumps has been a major contribution in our understanding of the multidrug resistance phenotype of cancer cells. Adenosine Triphosphate 66-69 ATP binding cassette subfamily B member 1 Homo sapiens 114-118 11498397-1 2000 The identification of certain members of the large superfamily of ATP binding cassette transport proteins such as MDR1 -P-glycoprotein and the multidrug resistance protein MRP1 as ATP-dependent drug efflux pumps has been a major contribution in our understanding of the multidrug resistance phenotype of cancer cells. Adenosine Triphosphate 66-69 ATP binding cassette subfamily B member 1 Homo sapiens 120-134 11498397-1 2000 The identification of certain members of the large superfamily of ATP binding cassette transport proteins such as MDR1 -P-glycoprotein and the multidrug resistance protein MRP1 as ATP-dependent drug efflux pumps has been a major contribution in our understanding of the multidrug resistance phenotype of cancer cells. Adenosine Triphosphate 180-183 ATP binding cassette subfamily B member 1 Homo sapiens 114-118 11498397-1 2000 The identification of certain members of the large superfamily of ATP binding cassette transport proteins such as MDR1 -P-glycoprotein and the multidrug resistance protein MRP1 as ATP-dependent drug efflux pumps has been a major contribution in our understanding of the multidrug resistance phenotype of cancer cells. Adenosine Triphosphate 180-183 ATP binding cassette subfamily B member 1 Homo sapiens 120-134 11006111-1 2000 P-glycoprotein (P-gp) is an ATP-dependent drug pump that confers multidrug resistance. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 10972658-6 2000 It has also been suggested that ATP-dependent primary active transporters such as MDR1/P-glycoprotein and the multidrug resistance-associated protein (MRP) gene family function as efflux pumps of renal tubular cells for more hydrophobic molecules and anionic conjugates. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 82-86 10972658-6 2000 It has also been suggested that ATP-dependent primary active transporters such as MDR1/P-glycoprotein and the multidrug resistance-associated protein (MRP) gene family function as efflux pumps of renal tubular cells for more hydrophobic molecules and anionic conjugates. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 87-101 10962093-0 2000 Two transport binding sites of P-glycoprotein are unequal yet contingent: initial rate kinetic analysis by ATP hydrolysis demonstrates intersite dependence. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 31-45 10962093-1 2000 The ATP-dependent transport enzyme known as P-glycoprotein (P-gp) confers multidrug resistance (MDR) against many unrelated drugs and xenobiotics. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 44-58 10962093-1 2000 The ATP-dependent transport enzyme known as P-glycoprotein (P-gp) confers multidrug resistance (MDR) against many unrelated drugs and xenobiotics. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 60-64 10716986-0 2000 Evidence for a requirement for ATP hydrolysis at two distinct steps during a single turnover of the catalytic cycle of human P-glycoprotein. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 125-139 10733510-1 2000 Multidrug resistance (MDR) is often characterized by the expression of P-glycoprotein (P-gp), a 170-kd ATP-dependent drug efflux protein. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 71-85 10733510-1 2000 Multidrug resistance (MDR) is often characterized by the expression of P-glycoprotein (P-gp), a 170-kd ATP-dependent drug efflux protein. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 10717249-4 2000 Two plasma membrane proteins, p-glycoprotein (p-gp) and the multidrug resistance-associated protein (MRP), act as ATP-dependent cellular efflux. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 30-44 10717249-4 2000 Two plasma membrane proteins, p-glycoprotein (p-gp) and the multidrug resistance-associated protein (MRP), act as ATP-dependent cellular efflux. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 46-50 11475537-3 2000 Pgp belongs to the ATP-binding cassette (ABC) family of transporter molecules which require hydrolysis of ATP to run the transport mechanism. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 10806188-1 2000 P-glycoprotein (P-gp) is an ATP-dependent drug pump that contains two nucleotide-binding domains (NBDs). Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 10806188-1 2000 P-glycoprotein (P-gp) is an ATP-dependent drug pump that contains two nucleotide-binding domains (NBDs). Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 10747957-1 2000 Multidrug resistance protein 1 (MRP1) and P-glycoprotein, which are ATP-dependent multidrug efflux pumps and involved in multidrug resistance of tumor cells, are members of the ATP binding cassette proteins and contain two nucleotide-binding folds (NBFs). Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 42-56 10747957-1 2000 Multidrug resistance protein 1 (MRP1) and P-glycoprotein, which are ATP-dependent multidrug efflux pumps and involved in multidrug resistance of tumor cells, are members of the ATP binding cassette proteins and contain two nucleotide-binding folds (NBFs). Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 0-30 10747957-1 2000 Multidrug resistance protein 1 (MRP1) and P-glycoprotein, which are ATP-dependent multidrug efflux pumps and involved in multidrug resistance of tumor cells, are members of the ATP binding cassette proteins and contain two nucleotide-binding folds (NBFs). Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 32-36 10747957-1 2000 Multidrug resistance protein 1 (MRP1) and P-glycoprotein, which are ATP-dependent multidrug efflux pumps and involved in multidrug resistance of tumor cells, are members of the ATP binding cassette proteins and contain two nucleotide-binding folds (NBFs). Adenosine Triphosphate 177-180 ATP binding cassette subfamily B member 1 Homo sapiens 0-30 10747957-1 2000 Multidrug resistance protein 1 (MRP1) and P-glycoprotein, which are ATP-dependent multidrug efflux pumps and involved in multidrug resistance of tumor cells, are members of the ATP binding cassette proteins and contain two nucleotide-binding folds (NBFs). Adenosine Triphosphate 177-180 ATP binding cassette subfamily B member 1 Homo sapiens 32-36 10747957-1 2000 Multidrug resistance protein 1 (MRP1) and P-glycoprotein, which are ATP-dependent multidrug efflux pumps and involved in multidrug resistance of tumor cells, are members of the ATP binding cassette proteins and contain two nucleotide-binding folds (NBFs). Adenosine Triphosphate 177-180 ATP binding cassette subfamily B member 1 Homo sapiens 42-56 10747957-2 2000 P-glycoprotein hydrolyzes ATP at both NBFs, and vanadate-induced nucleotide trapping occurs at both NBFs. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 10820025-2 2000 In the present study, we explored the nature of Mg(2+)-ATP binding in the N-terminal nucleotide-binding domain of human P-glycoprotein and identified the minimal functional unit required for specific ATP binding. Adenosine Triphosphate 55-58 ATP binding cassette subfamily B member 1 Homo sapiens 120-134 10820025-2 2000 In the present study, we explored the nature of Mg(2+)-ATP binding in the N-terminal nucleotide-binding domain of human P-glycoprotein and identified the minimal functional unit required for specific ATP binding. Adenosine Triphosphate 200-203 ATP binding cassette subfamily B member 1 Homo sapiens 120-134 10820025-10 2000 Taken together, these data define the region of the N-terminal nucleotide-binding domain of P-glycoprotein that is required for specific ATP binding and suggest that magnesium may play a role in stabilizing the ATP-binding site. Adenosine Triphosphate 137-140 ATP binding cassette subfamily B member 1 Homo sapiens 92-106 10820025-10 2000 Taken together, these data define the region of the N-terminal nucleotide-binding domain of P-glycoprotein that is required for specific ATP binding and suggest that magnesium may play a role in stabilizing the ATP-binding site. Adenosine Triphosphate 211-214 ATP binding cassette subfamily B member 1 Homo sapiens 92-106 10727237-1 2000 The human MDR1 gene product, P-glycoprotein (Pgp), a tandemly duplicated molecule containing two putative ATP- and perhaps two drug-binding sites, is responsible for multidrug resistance in tumors. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 10-14 10727237-1 2000 The human MDR1 gene product, P-glycoprotein (Pgp), a tandemly duplicated molecule containing two putative ATP- and perhaps two drug-binding sites, is responsible for multidrug resistance in tumors. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 29-43 10727237-1 2000 The human MDR1 gene product, P-glycoprotein (Pgp), a tandemly duplicated molecule containing two putative ATP- and perhaps two drug-binding sites, is responsible for multidrug resistance in tumors. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 45-48 10716986-1 2000 P-glycoprotein (Pgp) is an ATP-dependent hydrophobic natural product anticancer drug efflux pump whose overexpression confers multidrug resistance to tumor cells. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 10716986-1 2000 P-glycoprotein (Pgp) is an ATP-dependent hydrophobic natural product anticancer drug efflux pump whose overexpression confers multidrug resistance to tumor cells. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 10716986-4 2000 Though binding of nucleotide per se does not affect interactions with the substrate, ATP hydrolysis results in a dramatic conformational change where the affinity of [(125)I]iodoarylazidoprazoin for Pgp trapped in transition-state conformation (Pgp x ADP x vanadate) is reduced >30-fold. Adenosine Triphosphate 85-88 ATP binding cassette subfamily B member 1 Homo sapiens 199-202 10716986-4 2000 Though binding of nucleotide per se does not affect interactions with the substrate, ATP hydrolysis results in a dramatic conformational change where the affinity of [(125)I]iodoarylazidoprazoin for Pgp trapped in transition-state conformation (Pgp x ADP x vanadate) is reduced >30-fold. Adenosine Triphosphate 85-88 ATP binding cassette subfamily B member 1 Homo sapiens 245-248 10716986-5 2000 To transform Pgp from this intermediate state of low affinity for substrate to the next catalytic cycle, i.e., a conformation that binds substrate with high affinity, requires conditions that permit ATP hydrolysis. Adenosine Triphosphate 199-202 ATP binding cassette subfamily B member 1 Homo sapiens 13-16 10716986-10 2000 Our data demonstrate two distinct roles for ATP hydrolysis in a single turnover of the catalytic cycle of Pgp, one in the transport of substrate and the other in effecting conformational changes to reset the pump for the next catalytic cycle. Adenosine Triphosphate 44-47 ATP binding cassette subfamily B member 1 Homo sapiens 106-109 10708946-2 2000 Pgp and MRP1 function as ATP-dependent efflux pumps that extrude cytotoxic drugs from tumour cells. Adenosine Triphosphate 25-28 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 10739116-8 2000 Furthermore, in membrane fragments [3H]-vinblastine binding to P-glycoprotein was potently inhibited by both N-methyl-DN (Ki 10.7 +/- 4.9 nM) and DN (Ki 11.2 +/- 3.8 nM), and both N-methyl-DN and DN blocked ATP-dependent [3H]-vinblastine transport into inside-out vesicles. Adenosine Triphosphate 207-210 ATP binding cassette subfamily B member 1 Homo sapiens 63-77 10706193-1 2000 Transport by ATP-dependent efflux pumps such as P-glycoprotein is an increasingly recognized determinant of drug disposition. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 10667916-1 2000 P-glycoprotein (P-gp), the product of human MDR1 gene, which functions as an ATP-dependent drug efflux pump, is N-linked glycosylated at asparagine residues 91, 94, and 99 located within the first extracellular loop. Adenosine Triphosphate 77-80 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 10667916-1 2000 P-glycoprotein (P-gp), the product of human MDR1 gene, which functions as an ATP-dependent drug efflux pump, is N-linked glycosylated at asparagine residues 91, 94, and 99 located within the first extracellular loop. Adenosine Triphosphate 77-80 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 10667916-1 2000 P-glycoprotein (P-gp), the product of human MDR1 gene, which functions as an ATP-dependent drug efflux pump, is N-linked glycosylated at asparagine residues 91, 94, and 99 located within the first extracellular loop. Adenosine Triphosphate 77-80 ATP binding cassette subfamily B member 1 Homo sapiens 44-48 11076400-1 2000 The bile canaliculus contains at least four ATP-binding cassette (ABC) proteins responsible for ATP-dependent transport of bile acids (spgp), nonbile acid organic anions (mrp2), organic cations (mdr1), and phosphatidylcholine (mdr2). Adenosine Triphosphate 44-47 ATP binding cassette subfamily B member 1 Homo sapiens 195-199 10581365-1 1999 The P-glycoprotein multidrug transporter is a 170-kDa efflux pump which exports a diverse group of natural products, chemotherapeutic drugs, and hydrophobic peptides across the plasma membrane, driven by ATP hydrolysis. Adenosine Triphosphate 204-207 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 10574703-15 1999 In conclusion, in WIF-B cells, there is a direct route from Golgi to the canalicular membrane for trafficking of mdr1, a bile canalicular ATP-dependent transporter of organic cations. Adenosine Triphosphate 138-141 ATP binding cassette subfamily B member 1 Homo sapiens 113-117 10632321-3 1999 One such protein is P-glycoprotein (P-gp), which functions as an ATP-dependent active transporter. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 20-34 10632321-3 1999 One such protein is P-glycoprotein (P-gp), which functions as an ATP-dependent active transporter. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 36-40 10529234-0 1999 Both ATP sites of human P-glycoprotein are essential but not symmetric. Adenosine Triphosphate 5-8 ATP binding cassette subfamily B member 1 Homo sapiens 24-38 10513988-8 1999 Quaternary analogs, which are restricted by a permanent positive charge in transiting the plasma membrane by diffusion, accumulated in Pgp containing I/O vesicles in an ATP-dependent and cyclosporin A-inhibitable manner, which identified them as Pgp substrates. Adenosine Triphosphate 169-172 ATP binding cassette subfamily B member 1 Homo sapiens 135-138 10513988-8 1999 Quaternary analogs, which are restricted by a permanent positive charge in transiting the plasma membrane by diffusion, accumulated in Pgp containing I/O vesicles in an ATP-dependent and cyclosporin A-inhibitable manner, which identified them as Pgp substrates. Adenosine Triphosphate 169-172 ATP binding cassette subfamily B member 1 Homo sapiens 246-249 10529234-6 1999 [alpha-(32)P]-8-Azido-ATP labeling at 4 degrees C was inhibited in a concentration-dependent manner by ATP with half-maximal inhibition at approximately 10-20 microM for the P-gp-D1200N mutant and wild-type P-gp. Adenosine Triphosphate 22-25 ATP binding cassette subfamily B member 1 Homo sapiens 174-178 10529234-6 1999 [alpha-(32)P]-8-Azido-ATP labeling at 4 degrees C was inhibited in a concentration-dependent manner by ATP with half-maximal inhibition at approximately 10-20 microM for the P-gp-D1200N mutant and wild-type P-gp. Adenosine Triphosphate 22-25 ATP binding cassette subfamily B member 1 Homo sapiens 207-211 10491157-0 1999 Co-operating ATP sites in the multiple drug resistance transporter Mdr1. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 67-71 10491157-1 1999 The ATPase activity of the multiple drug resistance transporter Mdr1 (P-glycoprotein, gp170) depended on the concentration of ATP with both positive and negative co-operativity both in the absence and in the presence of verapamil. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 64-68 10491157-1 1999 The ATPase activity of the multiple drug resistance transporter Mdr1 (P-glycoprotein, gp170) depended on the concentration of ATP with both positive and negative co-operativity both in the absence and in the presence of verapamil. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 70-84 10491157-1 1999 The ATPase activity of the multiple drug resistance transporter Mdr1 (P-glycoprotein, gp170) depended on the concentration of ATP with both positive and negative co-operativity both in the absence and in the presence of verapamil. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 86-91 10491158-1 1999 Suramin, a known inhibitor of ATP binding enzymes with six negatively charged sulfonic acid groups, stimulated the ATPase activity of the multiple drug resistance transporter Mdr1 in low concentrations by acting as a substrate and by increasing the affinity for both verapamil and ATP. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 175-179 10491158-1 1999 Suramin, a known inhibitor of ATP binding enzymes with six negatively charged sulfonic acid groups, stimulated the ATPase activity of the multiple drug resistance transporter Mdr1 in low concentrations by acting as a substrate and by increasing the affinity for both verapamil and ATP. Adenosine Triphosphate 115-118 ATP binding cassette subfamily B member 1 Homo sapiens 175-179 10496352-1 1999 The multidrug resistance protein 1 (MRP1) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 4-34 10570926-5 1999 These results suggest that NBF1 of SUR1 has a NEM-sensitive structure similar to that of NBF1 of MDR1, a multidrug transporter, and confirm NBF1 as the high-affinity ATP binding site on SUR1. Adenosine Triphosphate 166-169 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 10493948-1 1999 P-glycoprotein (Pgp) is a plasma-membrane glycoprotein that confers multi-drug resistance (MDR) on cells and displays ATP-driven drug pumping. Adenosine Triphosphate 118-121 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 10493948-1 1999 P-glycoprotein (Pgp) is a plasma-membrane glycoprotein that confers multi-drug resistance (MDR) on cells and displays ATP-driven drug pumping. Adenosine Triphosphate 118-121 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 10496352-1 1999 The multidrug resistance protein 1 (MRP1) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 36-40 10429507-1 1999 The most well-characterized mechanism of multidrug resistance (MDR) involves P-glycoprotein (Pgp), a transmembrane protein acting as an ATP-dependent drug efflux pump. Adenosine Triphosphate 136-139 ATP binding cassette subfamily B member 1 Homo sapiens 77-91 10454753-1 1999 Resistance to chemotherapy in cancer cells is mainly mediated by overexpression of P-glycoprotein (Pgp), a plasma membrane ATP-binding cassette (ABC) transporter which extrudes cytotoxic drugs at the expense of ATP hydrolysis. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 83-97 10454753-1 1999 Resistance to chemotherapy in cancer cells is mainly mediated by overexpression of P-glycoprotein (Pgp), a plasma membrane ATP-binding cassette (ABC) transporter which extrudes cytotoxic drugs at the expense of ATP hydrolysis. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 99-102 10454753-2 1999 Pgp consists of two homologous halves each containing a transmembrane domain and a cytosolic nucleotide-binding domain (NBD) which contains two consensus Walker motifs, A and B, involved in ATP binding and hydrolysis. Adenosine Triphosphate 190-193 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 10411602-1 1999 Overexpression of ATP-dependent drug efflux pumps, P-glycoprotein (Pgp) or multidrug resistance-associated protein (MRP), confers multidrug resistance to tumor cells. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 51-65 10411602-1 1999 Overexpression of ATP-dependent drug efflux pumps, P-glycoprotein (Pgp) or multidrug resistance-associated protein (MRP), confers multidrug resistance to tumor cells. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 67-70 10450921-5 1999 While free ADR up-regulated genes encoding ATP driven efflux pumps (MDR1, MRP), P(GFLG)-ADR overcame existing pumps and down regulated the MRP gene. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 68-72 10364203-7 1999 Different conformational states were adopted by P-glycoprotein upon the addition of the anthracycline derivatives in the absence and presence of MgATP or MgATPgammaS. Adenosine Triphosphate 145-150 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 10376151-1 1999 The multidrug resistance (MDR1) gene product, P-glycoprotein (Pgp), is a 170-kDa ATP-dependent pump that expels a variety of anticancer drugs out of malignant cells, reducing drug accumulation and thus antitumor activity. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 26-30 10695015-1 1999 MDR results from overexpression of P-glycoprotein (Pgp) and multidrug resistance protein (MRP or MRP1) that function as ATP-dependent efflux pumps. Adenosine Triphosphate 120-123 ATP binding cassette subfamily B member 1 Homo sapiens 35-49 10695015-1 1999 MDR results from overexpression of P-glycoprotein (Pgp) and multidrug resistance protein (MRP or MRP1) that function as ATP-dependent efflux pumps. Adenosine Triphosphate 120-123 ATP binding cassette subfamily B member 1 Homo sapiens 51-54 10364203-3 1999 Trp quenching experiments provided evidence that P-glycoprotein adopts different tertiary structures upon binding of drug substrates in the absence and presence of MgATP and its nonhydrolyzable analog, MgATPgammaS. Adenosine Triphosphate 164-169 ATP binding cassette subfamily B member 1 Homo sapiens 49-63 10376151-1 1999 The multidrug resistance (MDR1) gene product, P-glycoprotein (Pgp), is a 170-kDa ATP-dependent pump that expels a variety of anticancer drugs out of malignant cells, reducing drug accumulation and thus antitumor activity. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 46-60 10376151-1 1999 The multidrug resistance (MDR1) gene product, P-glycoprotein (Pgp), is a 170-kDa ATP-dependent pump that expels a variety of anticancer drugs out of malignant cells, reducing drug accumulation and thus antitumor activity. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 62-65 10429507-1 1999 The most well-characterized mechanism of multidrug resistance (MDR) involves P-glycoprotein (Pgp), a transmembrane protein acting as an ATP-dependent drug efflux pump. Adenosine Triphosphate 136-139 ATP binding cassette subfamily B member 1 Homo sapiens 93-96 10366541-5 1999 DNP-SG efflux in MRP1-expressing MCF7 cells was ATP-dependent and exhibited an apparent Km for DNP-SG of 95 microM. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 17-21 10075817-5 1999 The fluorescein-colchicine transport was found to be dependent on the presence of P-glycoprotein, required ATP, and was inhibited by vanadate and the reversal agent, verapamil, in a dose-dependent manner. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 82-96 10385244-2 1999 The intracellular transport of leukotriene C4 (LTC4) in hematopoietic cells such as human monocytes is controlled by an ATP dependent carrier encoded by the multidrug resistance protein1 (MRPI) gene whose function can be blocked by the compound MK-571. Adenosine Triphosphate 120-123 ATP binding cassette subfamily B member 1 Homo sapiens 157-186 10385244-2 1999 The intracellular transport of leukotriene C4 (LTC4) in hematopoietic cells such as human monocytes is controlled by an ATP dependent carrier encoded by the multidrug resistance protein1 (MRPI) gene whose function can be blocked by the compound MK-571. Adenosine Triphosphate 120-123 ATP binding cassette subfamily B member 1 Homo sapiens 188-192 10092872-2 1999 P-glycoprotein is a plasma membrane protein of mammalian cells that confers multidrug resistance by acting as a broad-specificity, ATP-dependent efflux transporter of diverse lipophilic neutral or cationic compounds. Adenosine Triphosphate 131-134 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 10098736-7 1999 The high potency and selectivity of GG918 for the inhibition of human MDR1 and rat Mdr1b P-glycoprotein may serve to interfere with this type of multidrug resistance and provides a tool for studies on the function of these ATP-dependent transport proteins. Adenosine Triphosphate 223-226 ATP binding cassette subfamily B member 1 Homo sapiens 70-74 21374027-6 1999 The measurement of the active (adenosine triphosphate [ATP]-dependent) drug transport or efflux function of Pgp is, therefore, a theoretically elegant way to quantify the number of active or "functional" Pgp molecules per cell (2,3). Adenosine Triphosphate 31-53 ATP binding cassette subfamily B member 1 Homo sapiens 108-111 10500793-3 1999 Known mechanisms of MDR are overexpression of the ATP-dependent membrane proteins P-glycoprotein (P-gp) and multidrug resistance protein (MRP1), or an increased detoxification of compounds mediated by glutathione (GSH) or GSH related enzymes. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 82-96 10500793-3 1999 Known mechanisms of MDR are overexpression of the ATP-dependent membrane proteins P-glycoprotein (P-gp) and multidrug resistance protein (MRP1), or an increased detoxification of compounds mediated by glutathione (GSH) or GSH related enzymes. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 98-102 10331240-6 1999 In a manner similar to ion-transporting ATPases, such as Na+,K(+)-ATPase, Pgp transports close to one drug molecule per ATP molecule hydrolyzed. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 74-77 21374027-6 1999 The measurement of the active (adenosine triphosphate [ATP]-dependent) drug transport or efflux function of Pgp is, therefore, a theoretically elegant way to quantify the number of active or "functional" Pgp molecules per cell (2,3). Adenosine Triphosphate 31-53 ATP binding cassette subfamily B member 1 Homo sapiens 204-207 21374027-6 1999 The measurement of the active (adenosine triphosphate [ATP]-dependent) drug transport or efflux function of Pgp is, therefore, a theoretically elegant way to quantify the number of active or "functional" Pgp molecules per cell (2,3). Adenosine Triphosphate 55-58 ATP binding cassette subfamily B member 1 Homo sapiens 108-111 21374027-6 1999 The measurement of the active (adenosine triphosphate [ATP]-dependent) drug transport or efflux function of Pgp is, therefore, a theoretically elegant way to quantify the number of active or "functional" Pgp molecules per cell (2,3). Adenosine Triphosphate 55-58 ATP binding cassette subfamily B member 1 Homo sapiens 204-207 9841738-2 1998 Resistance is manifested largely by the product of the human MDR1 gene, P-glycoprotein, an ABC transporter that is an integral membrane protein of 1280 amino acids arranged into two homologous halves, each comprising 6 putative transmembrane alpha-helices and an ATP binding domain. Adenosine Triphosphate 263-266 ATP binding cassette subfamily B member 1 Homo sapiens 61-65 9819232-1 1998 P-glycoprotein (Pgp), the product of the MDR1 gene, confers multidrug resistance on cancer cells by ATP-dependent extrusion of anticancer drugs. Adenosine Triphosphate 100-103 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9819232-1 1998 P-glycoprotein (Pgp), the product of the MDR1 gene, confers multidrug resistance on cancer cells by ATP-dependent extrusion of anticancer drugs. Adenosine Triphosphate 100-103 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 9819232-1 1998 P-glycoprotein (Pgp), the product of the MDR1 gene, confers multidrug resistance on cancer cells by ATP-dependent extrusion of anticancer drugs. Adenosine Triphosphate 100-103 ATP binding cassette subfamily B member 1 Homo sapiens 41-45 9841738-2 1998 Resistance is manifested largely by the product of the human MDR1 gene, P-glycoprotein, an ABC transporter that is an integral membrane protein of 1280 amino acids arranged into two homologous halves, each comprising 6 putative transmembrane alpha-helices and an ATP binding domain. Adenosine Triphosphate 263-266 ATP binding cassette subfamily B member 1 Homo sapiens 72-86 9753453-0 1998 Structural flexibility of the linker region of human P-glycoprotein permits ATP hydrolysis and drug transport. Adenosine Triphosphate 76-79 ATP binding cassette subfamily B member 1 Homo sapiens 53-67 9778376-1 1998 P-glycoprotein (Pgp) mediates drug transport utilizing the energy released from ATP hydrolysis. Adenosine Triphosphate 80-83 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9778376-1 1998 P-glycoprotein (Pgp) mediates drug transport utilizing the energy released from ATP hydrolysis. Adenosine Triphosphate 80-83 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 9778376-4 1998 Preincubation of human Pgp expressed in Sf9 insect cells with vanadate in the presence of Mg2+, ATP, and verapamil resulted in nearly complete and stable inhibition of the drug-stimulated ATPase function. Adenosine Triphosphate 96-99 ATP binding cassette subfamily B member 1 Homo sapiens 23-26 9778376-5 1998 In contrast, the Pgp ATPase function was nearly unaffected when Mg2+, ATP, or verapamil was omitted. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 17-20 9778376-6 1998 Inhibition was highly specific for divalent cations that support ATP hydrolysis, for nucleotides that serve as substrates of hydrolysis, and for those drugs/compounds that interact with the drug-binding/transport sites of the Pgp. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 226-229 9753453-10 1998 Taken together, these data suggest a flexible secondary structure of the connector region is sufficient for the coordinate functioning of the two halves of Pgp, likely specifically required for the proper interaction of the two ATP binding sites. Adenosine Triphosphate 228-231 ATP binding cassette subfamily B member 1 Homo sapiens 156-159 19002782-5 1998 P-glycoprotein consists of two highly homologous halves each of which contains a transmembrane domain and an ATP binding fold. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 19002782-8 1998 This review summarizes the current status of knowledge on the domain organization, topology and higher order structure of P-glycoprotein, the location of drug- and ATP binding sites within P-glycoprotein, its ATPase and drug transport activities, its possible functions as an ion channel, ATP channel and lipid transporter, its potential role in cholesterol biosynthesis, and the effects of phosphorylation on P-glycoprotein activity. Adenosine Triphosphate 164-167 ATP binding cassette subfamily B member 1 Homo sapiens 189-203 19002782-8 1998 This review summarizes the current status of knowledge on the domain organization, topology and higher order structure of P-glycoprotein, the location of drug- and ATP binding sites within P-glycoprotein, its ATPase and drug transport activities, its possible functions as an ion channel, ATP channel and lipid transporter, its potential role in cholesterol biosynthesis, and the effects of phosphorylation on P-glycoprotein activity. Adenosine Triphosphate 164-167 ATP binding cassette subfamily B member 1 Homo sapiens 189-203 9746361-5 1998 The kinetic parameters for P-glycoprotein ATPase activity were determined, and a fluorescence-quenching technique was used to measure the Kd for ATP binding. Adenosine Triphosphate 42-45 ATP binding cassette subfamily B member 1 Homo sapiens 27-41 9746361-0 1998 The ATPase and ATP-binding functions of P-glycoprotein--modulation by interaction with defined phospholipids. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 40-54 9841506-1 1998 P-glycoprotein (Mdr1p) is an ATP-dependent drug efflux pump that is overexpressed in multidrug-resistant cells and some cancers. Adenosine Triphosphate 29-32 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 19002778-4 1998 The P-glycoprotein is a member of a large superfamily of transport proteins which utilize ATP to translocate a wide range of substrates across biological membranes. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 19002782-4 1998 Overexpression of this 170 kDa N-glycosylated plasma membrane protein in mammalian cells has been associated with ATP-dependent reduced drug accumulation, suggesting that P-glycoprotein may act as an energy-dependent drug efflux pump. Adenosine Triphosphate 114-117 ATP binding cassette subfamily B member 1 Homo sapiens 171-185 9677321-2 1998 Natural products and chemotherapeutic drugs are pumped out of the cell by P-glycoprotein in an ATP-dependent fashion. Adenosine Triphosphate 95-98 ATP binding cassette subfamily B member 1 Homo sapiens 74-88 9716379-5 1998 However, how ATP hydrolysis relates to drug binding by Pgp is yet to be determined. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 55-58 9642211-2 1998 Human P-glycoprotein (P-gp), an ATP-dependent efflux pump responsible for cross-resistance of human cancers to a variety of lipophilic compounds, is composed of two homologous halves, each containing six transmembrane domains and an ATP-binding/utilization domain. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 6-20 9642211-2 1998 Human P-glycoprotein (P-gp), an ATP-dependent efflux pump responsible for cross-resistance of human cancers to a variety of lipophilic compounds, is composed of two homologous halves, each containing six transmembrane domains and an ATP-binding/utilization domain. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 9642211-2 1998 Human P-glycoprotein (P-gp), an ATP-dependent efflux pump responsible for cross-resistance of human cancers to a variety of lipophilic compounds, is composed of two homologous halves, each containing six transmembrane domains and an ATP-binding/utilization domain. Adenosine Triphosphate 233-236 ATP binding cassette subfamily B member 1 Homo sapiens 6-20 9642211-2 1998 Human P-glycoprotein (P-gp), an ATP-dependent efflux pump responsible for cross-resistance of human cancers to a variety of lipophilic compounds, is composed of two homologous halves, each containing six transmembrane domains and an ATP-binding/utilization domain. Adenosine Triphosphate 233-236 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 9642211-6 1998 Reconstituted P-gp was incubated with either ATP or 8-azido-ATP in the presence or absence of Vi under ultraviolet (365 nm) light on ice for 60 min. Adenosine Triphosphate 45-48 ATP binding cassette subfamily B member 1 Homo sapiens 14-18 9923970-1 1998 P-glycoprotein (P-gp), a plasma membrane glycoprotein associated with the multidrug resistance phenotype, is responsible for the ATP-dependent efflux of various amphiphilic drugs. Adenosine Triphosphate 129-132 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9923970-1 1998 P-glycoprotein (P-gp), a plasma membrane glycoprotein associated with the multidrug resistance phenotype, is responsible for the ATP-dependent efflux of various amphiphilic drugs. Adenosine Triphosphate 129-132 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 9630701-8 1998 This provides a good framework for precise 3D structure modeling of Pgp by homology with proteins of known 3D structure, as it is illustrated here for the A motifs of the ATP-binding domains of Pgp. Adenosine Triphosphate 171-174 ATP binding cassette subfamily B member 1 Homo sapiens 68-71 9630701-8 1998 This provides a good framework for precise 3D structure modeling of Pgp by homology with proteins of known 3D structure, as it is illustrated here for the A motifs of the ATP-binding domains of Pgp. Adenosine Triphosphate 171-174 ATP binding cassette subfamily B member 1 Homo sapiens 194-197 9746361-6 1998 The values of both the Km for ATP hydrolysis and Kd for ATP binding were significantly different above and below the gel/liquid-crystalline phase transition temperature (tm) of PamMyrGroPCho and Myr2GroPCho, whereas they were similar at the same temperatures for P-glycoprotein in detergent solution. Adenosine Triphosphate 56-59 ATP binding cassette subfamily B member 1 Homo sapiens 263-277 9746361-10 1998 These results indicate that both ATP binding and ATP hydrolysis by P-glycoprotein are affected by the phase state of the host lipids in which it is reconstituted. Adenosine Triphosphate 33-36 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 9746361-10 1998 These results indicate that both ATP binding and ATP hydrolysis by P-glycoprotein are affected by the phase state of the host lipids in which it is reconstituted. Adenosine Triphosphate 49-52 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 9553060-2 1998 The human multidrug transporter (MDR1 or P-glycoprotein) is an ATP-dependent cellular drug extrusion pump, and its function involves a drug-stimulated, vanadate-inhibited ATPase activity. Adenosine Triphosphate 63-66 ATP binding cassette subfamily B member 1 Homo sapiens 33-37 9572868-1 1998 Very little structural information is available for P-glycoprotein (Pgp), which has been implicated in the multidrug resistance of human tumors because of its ability to act as an ATP-driven efflux pump for hydrophobic compounds. Adenosine Triphosphate 180-183 ATP binding cassette subfamily B member 1 Homo sapiens 52-66 9572868-1 1998 Very little structural information is available for P-glycoprotein (Pgp), which has been implicated in the multidrug resistance of human tumors because of its ability to act as an ATP-driven efflux pump for hydrophobic compounds. Adenosine Triphosphate 180-183 ATP binding cassette subfamily B member 1 Homo sapiens 68-71 9572868-9 1998 Following the addition of ATP to MIANS-Pgp, the NBD-PE quench curve no longer conformed to the models. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 39-42 9572868-10 1998 These results imply that Pgp interacts differently with PE when it is in the ATP-bound form. Adenosine Triphosphate 77-80 ATP binding cassette subfamily B member 1 Homo sapiens 25-28 9553060-3 1998 In the presence of vanadate and MgATP, a nucleotide (ADP) is trapped in MDR1, which alters the drug binding properties of the protein. Adenosine Triphosphate 32-37 ATP binding cassette subfamily B member 1 Homo sapiens 72-76 9553060-7 1998 MDR1 variants with mutations of key lysine residues to methionines in the N-terminal or C-terminal nucleotide binding domains (K433M, K1076M, and K433M/K1076M), which bind but do not hydrolyze ATP, do not show nucleotide trapping either with or without the transported drug substrates. Adenosine Triphosphate 193-196 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 9553060-8 1998 These data indicate that vanadate-dependent nucleotide trapping reflects a drug-stimulated partial reaction of ATP hydrolysis by MDR1, which involves the cooperation of the two nucleotide binding domains. Adenosine Triphosphate 111-114 ATP binding cassette subfamily B member 1 Homo sapiens 129-133 9553060-9 1998 The analysis of this drug-dependent partial reaction may significantly help to characterize the substrate recognition and the ATP-dependent transport mechanism of the MDR1 pump protein. Adenosine Triphosphate 126-129 ATP binding cassette subfamily B member 1 Homo sapiens 167-171 9538020-6 1998 Upon reconstitution into phospholipid vesicles, purified Pgp exhibited specific binding to analogues of substrates and ATP in affinity labeling experiments and displayed a high level of drug-stimulated ATPase activity (specific activity ranging from 4.5 to 6.5 micromol min-1 mg-1). Adenosine Triphosphate 119-122 ATP binding cassette subfamily B member 1 Homo sapiens 57-60 9630701-1 1998 P-glycoprotein (Pgp) is a plasma membrane protein known as an ATP-dependent drug-efflux pump that confers multidrug resistance to tumor cells. Adenosine Triphosphate 62-65 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9630701-1 1998 P-glycoprotein (Pgp) is a plasma membrane protein known as an ATP-dependent drug-efflux pump that confers multidrug resistance to tumor cells. Adenosine Triphosphate 62-65 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 9538020-8 1998 Vanadate which is known to inhibit ATPase activity by trapping MgADP at the catalytic site inhibited photoaffinity labeling of Pgp with substrate analogues, [125I]iodoarylazidoprazosin and [3H]azidopine, only under ATP hydrolysis conditions. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 127-130 9538020-9 1998 Because vanadate-trapped Pgp is known to resemble the ADP and phosphate-bound catalytic transition state, our findings indicate that ATP hydrolysis results in a conformation with reduced affinity for substrates. Adenosine Triphosphate 133-136 ATP binding cassette subfamily B member 1 Homo sapiens 25-28 10353701-2 1998 A major cause of multidrug resistance is the overexpression of a 170-kDa plasma membrane protein, known as P-glycoprotein, which appears to function as an ATP-driven efflux pump with a very broad specificity for hydrophobic drugs, peptides, and natural products. Adenosine Triphosphate 155-158 ATP binding cassette subfamily B member 1 Homo sapiens 107-121 9538020-1 1998 Human P-glycoprotein (Pgp), a plasma membrane protein that confers multidrug resistance, functions as an ATP-dependent drug efflux pump. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 6-20 9538020-1 1998 Human P-glycoprotein (Pgp), a plasma membrane protein that confers multidrug resistance, functions as an ATP-dependent drug efflux pump. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 22-25 9538020-2 1998 Pgp contains two ATP binding/utilization sites and exhibits ATPase activity that is stimulated in the presence of substrates and modulating agents. Adenosine Triphosphate 17-20 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 9538020-4 1998 To understand the role of ATP hydrolysis in drug binding, it is necessary to develop methods for purifying and reconstituting Pgp that retains properties including stimulation of ATPase activity by known substrates to an extent similar to that in the native membrane. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 126-129 9531525-3 1998 P-gp functions as an ATP-dependent drug efflux pump to reduce intracellular cytotoxic concentrations. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 9506530-8 1998 This was corroborated by whole-cell patch-clamp experiments, where the C219 antibody, which is directed against the ATP-binding site of P-glycoprotein, significantly inhibited P-glycoprotein-associated, volume-activated chloride currents in quiescent, but not proliferating cells from multicellular tumor spheroids. Adenosine Triphosphate 116-119 ATP binding cassette subfamily B member 1 Homo sapiens 136-150 9506530-8 1998 This was corroborated by whole-cell patch-clamp experiments, where the C219 antibody, which is directed against the ATP-binding site of P-glycoprotein, significantly inhibited P-glycoprotein-associated, volume-activated chloride currents in quiescent, but not proliferating cells from multicellular tumor spheroids. Adenosine Triphosphate 116-119 ATP binding cassette subfamily B member 1 Homo sapiens 176-190 9635488-2 1998 The presence of this ATP-dependent membrane P-glycoprotein (Pgp) pump was confirmed by biochemical ("binding"), molecular (immunohistochemical, Western, Northern), physiological (verapamil-sensitivity) and toxicological (modulation of toxicity) methods. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 44-58 9635488-2 1998 The presence of this ATP-dependent membrane P-glycoprotein (Pgp) pump was confirmed by biochemical ("binding"), molecular (immunohistochemical, Western, Northern), physiological (verapamil-sensitivity) and toxicological (modulation of toxicity) methods. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 60-63 9530112-1 1998 Expression of the cystic fibrosis transmembrane conductance regulator (CFTR), and of at least one other member of the ATP-binding cassette family of transport proteins, P-glycoprotein, is associated with the electrodiffusional movement of the nucleotide ATP. Adenosine Triphosphate 118-121 ATP binding cassette subfamily B member 1 Homo sapiens 169-183 9396736-5 1997 Pgp has high basal ATPase activity; that is, ATP hydrolysis by Pgp is not rigidly associated with drug transport. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 9711564-0 1998 Photoaffinity labeling of human P-glycoprotein: effect of modulator interaction and ATP hydrolysis on substrate binding. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 32-46 9396736-5 1997 Pgp has high basal ATPase activity; that is, ATP hydrolysis by Pgp is not rigidly associated with drug transport. Adenosine Triphosphate 19-22 ATP binding cassette subfamily B member 1 Homo sapiens 63-66 9396736-9 1997 We found that generation of tryptic fragments, including the loop linking transmembrane (TM) regions TM8 and TM9 of Pgp, were stimulated by the binding of Mg-p[NH]ppA, MgATP and MgADP, indicating that the Pgp conformation was changed by the binding of these nucleotides. Adenosine Triphosphate 168-173 ATP binding cassette subfamily B member 1 Homo sapiens 116-119 9396736-9 1997 We found that generation of tryptic fragments, including the loop linking transmembrane (TM) regions TM8 and TM9 of Pgp, were stimulated by the binding of Mg-p[NH]ppA, MgATP and MgADP, indicating that the Pgp conformation was changed by the binding of these nucleotides. Adenosine Triphosphate 168-173 ATP binding cassette subfamily B member 1 Homo sapiens 205-208 9396736-12 1997 We propose that cycling through these four states couples the Pgp-mediated MgATP hydrolysis to drug transport. Adenosine Triphosphate 75-80 ATP binding cassette subfamily B member 1 Homo sapiens 62-65 9371774-3 1997 UIC2 reactivity with Pgp was increased by the addition of several Pgp-transported compounds or ATP-depleting agents, and by mutational inactivation of both nucleotide-binding domains (NBDs) of Pgp. Adenosine Triphosphate 95-98 ATP binding cassette subfamily B member 1 Homo sapiens 21-24 9422060-2 1997 P-glycoprotein and MRP function as ATP-dependent efflux pumps that extrude cytotoxic drugs from the cells before the drugs reach their intracellular targets, thus conferring resistance to many structurally dissimilar anti-cancer drugs. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9371774-5 1997 These results indicate the existence of different Pgp conformations associated with different stages of transport-associated ATP hydrolysis and suggest trapping in a transient conformation as a mechanism for antibody-mediated inhibition of Pgp. Adenosine Triphosphate 125-128 ATP binding cassette subfamily B member 1 Homo sapiens 50-53 9371774-5 1997 These results indicate the existence of different Pgp conformations associated with different stages of transport-associated ATP hydrolysis and suggest trapping in a transient conformation as a mechanism for antibody-mediated inhibition of Pgp. Adenosine Triphosphate 125-128 ATP binding cassette subfamily B member 1 Homo sapiens 240-243 9317175-1 1997 BACKGROUND: In cancer treated with chemotherapy, multidrug resistance is characterized by increased genetic expression of P-glycoprotein (P-gp), which acts as an ATP-dependent drug-efflux pump. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 122-136 9431999-1 1997 P-glycoprotein is an ATP-dependent plasma membrane multidrug transporter of broad specificity. Adenosine Triphosphate 21-24 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9431999-10 1997 The initial rate of Hoechst 33342 transport upon ATP addition to P-glycoprotein-rich inside-out plasma membrane vesicles decreased slightly with the amount of time previously elapsed for slow diffusion of Hoechst 33342 to the extracellular leaflet. Adenosine Triphosphate 49-52 ATP binding cassette subfamily B member 1 Homo sapiens 65-79 9317175-1 1997 BACKGROUND: In cancer treated with chemotherapy, multidrug resistance is characterized by increased genetic expression of P-glycoprotein (P-gp), which acts as an ATP-dependent drug-efflux pump. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 138-142 9287320-9 1997 The dependence of the MDR1-ATPase activity on ATP concentration was identical in the wild-type and the mutant proteins, and Hill plots indicated the presence of more than one functional ATP-binding site. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 9380680-1 1997 Human P-glycoprotein (Pgp) confers multidrug resistance to cancer cells by ATP-dependent extrusion of a great many structurally dissimilar hydrophobic compounds. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 6-20 9380680-1 1997 Human P-glycoprotein (Pgp) confers multidrug resistance to cancer cells by ATP-dependent extrusion of a great many structurally dissimilar hydrophobic compounds. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 22-25 9287320-9 1997 The dependence of the MDR1-ATPase activity on ATP concentration was identical in the wild-type and the mutant proteins, and Hill plots indicated the presence of more than one functional ATP-binding site. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 9313931-2 1997 P-glycoprotein, a 170-180 kDa membrane glycoprotein that mediates multidrug resistance, hydrolyses ATP to efflux a broad spectrum of hydrophobic agents. Adenosine Triphosphate 99-102 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9313931-13 1997 The binding of 8-azido-[alpha-32P]-ATP to P-glycoprotein was not altered by the cyclosporin derivatives, verapamil, vinblastine and doxorubicin, suggesting that the modulation by these agents of P-glycoprotein ATPase cannot be attributed to an effect on ATP binding to P-glycoprotein. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 42-56 9313931-13 1997 The binding of 8-azido-[alpha-32P]-ATP to P-glycoprotein was not altered by the cyclosporin derivatives, verapamil, vinblastine and doxorubicin, suggesting that the modulation by these agents of P-glycoprotein ATPase cannot be attributed to an effect on ATP binding to P-glycoprotein. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 195-209 9313931-13 1997 The binding of 8-azido-[alpha-32P]-ATP to P-glycoprotein was not altered by the cyclosporin derivatives, verapamil, vinblastine and doxorubicin, suggesting that the modulation by these agents of P-glycoprotein ATPase cannot be attributed to an effect on ATP binding to P-glycoprotein. Adenosine Triphosphate 35-38 ATP binding cassette subfamily B member 1 Homo sapiens 195-209 9332327-8 1997 We suggest that: (1) over-expression of Pgp by drug-resistant cells may up-regulate mitochondrial energy production; (2) mitochondrial ETC activity is required for DNA fragmentation in response to vinblastine, but the mechanism is independent of Pgp activity and ATP generation; (3) down-regulation of mitochondrial ETC activity may confer resistance to vinblastine-induced apoptosis; (4) the mitochondrial ETC is involved in vinblastine-induced apoptosis downstream of microtubule disruption and cell cycle changes. Adenosine Triphosphate 263-266 ATP binding cassette subfamily B member 1 Homo sapiens 40-43 28481204-3 1997 The unique functional features of this transporter include its ability to mediate ATP-dependent transmembrane movement of organic anions such as glutathione conjugates, as well as weakly cationic amphiphilic compounds such as doxorubicin and other substrates of P-glycoprotein. Adenosine Triphosphate 82-85 ATP binding cassette subfamily B member 1 Homo sapiens 262-276 9261097-1 1997 Transmembrane segments (TM) 6 and 12 are directly connected to the ATP-binding domain in each homologous half of P-glycoprotein and are postulated to be important for drug-protein interactions. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 113-127 9207250-3 1997 The P-glycoprotein in the placenta and mole was photolabeled with [3H]azidopine, and [3H]vincristine was transported in an ATP-dependent manner into membrane vesicles prepared from trophoblasts that expressed P-glycoprotein. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 209-223 9261121-0 1997 Relation between the turnover number for vinblastine transport and for vinblastine-stimulated ATP hydrolysis by human P-glycoprotein. Adenosine Triphosphate 94-97 ATP binding cassette subfamily B member 1 Homo sapiens 118-132 9261121-1 1997 Considerable uncertainty surrounds the stoichiometry of coupling of ATP hydrolysis to drug pumping by P-glycoprotein, the multidrug transporter. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 102-116 9261121-10 1997 The vinblastine-stimulated ATPase activity of P-glycoprotein has a turnover number of 3.5 s-1 at 37 degrees C, giving 2.8 molecules of ATP hydrolyzed for every vinblastine molecule transported in a particular direction. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 46-60 9261121-12 1997 Thus, ATP hydrolysis is probably directly linked to drug transport by P-glycoprotein. Adenosine Triphosphate 6-9 ATP binding cassette subfamily B member 1 Homo sapiens 70-84 9387101-5 1997 Two main actors of this multidrug phenotype (MDR) are represented by the P-glycoprotein and by the more recently discovered multidrug-resistance associated protein (MRP), two membrane proteins of the ABC superfamily of transporters that can extrude chemotherapeutic drugs under an ATP-dependent mechanism. Adenosine Triphosphate 281-284 ATP binding cassette subfamily B member 1 Homo sapiens 73-87 9256153-1 1997 P-Glycoprotein functions as an ATP-driven efflux pump for hydrophobic natural products and peptides, and gives rise to resistance to multiple chemotherapeutic drugs. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9441944-0 1997 ATP hydrolysis cycles and mechanism in P-glycoprotein and CFTR. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 39-53 9143365-3 1997 With MgATP or MgADP, reactivation occurred with t1/2 = 7 min at 37 degrees C. With 8-azido-ATP, UV irradiation of inhibited Pgp gave specific photolabeling of both nucleotide sites. Adenosine Triphosphate 5-10 ATP binding cassette subfamily B member 1 Homo sapiens 124-127 9186779-8 1997 Considering that an ATP-dependent process was involved in 59Fe release by PIH, further studies examined 4 widely used inhibitors of the multi-drug efflux pump P-glycoprotein (P-gp). Adenosine Triphosphate 20-23 ATP binding cassette subfamily B member 1 Homo sapiens 159-173 9186779-8 1997 Considering that an ATP-dependent process was involved in 59Fe release by PIH, further studies examined 4 widely used inhibitors of the multi-drug efflux pump P-glycoprotein (P-gp). Adenosine Triphosphate 20-23 ATP binding cassette subfamily B member 1 Homo sapiens 175-179 9141359-7 1997 A similar increase in volume sensitivity of the Cl- channel was also induced by the expression of the ATP hydrolysis-deficient PGP mutant, K433M. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 127-130 9163728-6 1997 The development of the ATP-dependent multidrug resistant phenotype was due to low to high levels of expression of P-glycoprotein (PGP). Adenosine Triphosphate 23-26 ATP binding cassette subfamily B member 1 Homo sapiens 114-128 9163728-6 1997 The development of the ATP-dependent multidrug resistant phenotype was due to low to high levels of expression of P-glycoprotein (PGP). Adenosine Triphosphate 23-26 ATP binding cassette subfamily B member 1 Homo sapiens 130-133 9235086-6 1997 The structure of p-glycoprotein consists in two symmetrical halves anchored in the cellular membrane that includes three extracellular dominances each one, and on intracellular portion with the ATP binding site. Adenosine Triphosphate 194-197 ATP binding cassette subfamily B member 1 Homo sapiens 17-31 9127318-2 1997 The product of this gene is the multidrug transporter P-glycoprotein, an ATP-dependent pump that extrudes drugs from the cytoplasm. Adenosine Triphosphate 73-76 ATP binding cassette subfamily B member 1 Homo sapiens 54-68 9119038-7 1997 Since these two molecules have different effects both on the basal P-glycoprotein ATPase activity and on the MgATP concentration dependence of P-glycoprotein ATPase activity, they could bind P-glycoprotein either on different and overlapping sites, or on distant but interacting sites. Adenosine Triphosphate 109-114 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 9119038-7 1997 Since these two molecules have different effects both on the basal P-glycoprotein ATPase activity and on the MgATP concentration dependence of P-glycoprotein ATPase activity, they could bind P-glycoprotein either on different and overlapping sites, or on distant but interacting sites. Adenosine Triphosphate 109-114 ATP binding cassette subfamily B member 1 Homo sapiens 143-157 9119038-7 1997 Since these two molecules have different effects both on the basal P-glycoprotein ATPase activity and on the MgATP concentration dependence of P-glycoprotein ATPase activity, they could bind P-glycoprotein either on different and overlapping sites, or on distant but interacting sites. Adenosine Triphosphate 109-114 ATP binding cassette subfamily B member 1 Homo sapiens 143-157 9016791-4 1997 The photolabeling of P-glycoprotein with 8-azido-[alpha-32P]ATP was inhibited by C219, suggesting that the inhibition of ATP binding by C219 reduced the activity. Adenosine Triphosphate 60-63 ATP binding cassette subfamily B member 1 Homo sapiens 21-35 9016791-1 1997 P-glycoprotein functions as an ATP-driven efflux pump for antitumor agents. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9016791-2 1997 C219 is a monoclonal antibody which recognizes regions near both ATP binding domains in each half of P-glycoprotein. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 101-115 9304397-2 1996 P-glycoprotein (P-gp) is a transmembrane protein involved in ATP-dependent efflux of various structurally unrelated anticancer drugs. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9304397-2 1996 P-glycoprotein (P-gp) is a transmembrane protein involved in ATP-dependent efflux of various structurally unrelated anticancer drugs. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 8910331-1 1996 Each homologous half of P-glycoprotein consists of a transmembrane domain with six potential transmembrane segments and an ATP-binding domain. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 24-38 9207250-3 1997 The P-glycoprotein in the placenta and mole was photolabeled with [3H]azidopine, and [3H]vincristine was transported in an ATP-dependent manner into membrane vesicles prepared from trophoblasts that expressed P-glycoprotein. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 9020050-2 1997 One theory concerning the mechanism of Pgp activity is that efflux of ATP is coupled to drug efflux. Adenosine Triphosphate 70-73 ATP binding cassette subfamily B member 1 Homo sapiens 39-42 8973548-1 1996 P-Glycoprotein functions as an ATP-driven active efflux pump for many natural products and chemotherapeutic drugs. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 8910534-1 1996 Plasma membrane P-glycoprotein is known as an ATP-dependent drug efflux pump that confers multidrug resistance to tumor cells. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 16-30 9042256-3 1996 One of the well known mechanisms leading to multidrug resistance is the over-expression of the mdr1 gene product, the 170 kDa membrane P-glycoprotein which is an ATP-driven efflux pump of xenobiotics. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 95-99 8953513-10 1996 ATP depletion by occlusion of the superior mesenteric artery resulted in a clear increase in epithelial permeability to vinblastine, but not to 3-O-methylglucose or acetamide, indicating that vinblastine is secreted by ATP-dependent P-glycoprotein into the lumen. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 233-247 8773513-2 1996 The human multidrug resistance (MDR1) gene encodes a 170-kD glycoprotein (P-gp), an ATP-dependent transmembrane efflux pump for many different cytotoxic drugs. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 32-36 8794769-1 1996 P-Glycoprotein is a member of the ABC superfamily of membrane transporters, and functions as an ATP-driven active efflux pump for natural products and chemotherapeutic drugs. Adenosine Triphosphate 96-99 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 8794769-8 1996 MIANS-labeled P-glycoprotein was still able to bind ATP, as demonstrated by quenching of the fluorescence, with a Kd of 0.46 mM. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 14-28 8861952-1 1996 Lactococcus lactis possesses an ATP-dependent drug extrusion system which shares functional properties with the mammalian multidrug resistance (MDR) transporter P-glycoprotein. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 161-175 8898356-6 1996 In crude membrane preparations from HOS cells, a higher level of basal Pgp-ATPase activity was observed for the V185 variant than for the wild-type, suggesting partial uncoupling of drug-dependent ATP hydrolysis by the mutant. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 71-74 7896810-5 1995 Because transport substrate-induced ATP hydrolysis is generally thought to reflect transport function, these data suggest that colchicine and verapamil are transported at an increased rate with Gly185-->Val substitution in the Pgp. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 230-233 8711682-2 1996 P-gp is a 170 kD transmembrane ATP dependent drug efflux pump which has been shown to be important in the resistance of some tumours to chemotherapy. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 8600291-1 1996 Multidrug resistance (MDR) to chemically unrelated therapeutic anticancer agents in mammalian cells is mediated by the overexpression of an ATP-dependent 150- to 180-kD membrane glycoprotein P-glycoprotein (P-gp). Adenosine Triphosphate 140-143 ATP binding cassette subfamily B member 1 Homo sapiens 191-205 8600291-1 1996 Multidrug resistance (MDR) to chemically unrelated therapeutic anticancer agents in mammalian cells is mediated by the overexpression of an ATP-dependent 150- to 180-kD membrane glycoprotein P-glycoprotein (P-gp). Adenosine Triphosphate 140-143 ATP binding cassette subfamily B member 1 Homo sapiens 207-211 8621717-0 1996 Functional reconstitution of P-glycoprotein reveals an apparent near stoichiometric drug transport to ATP hydrolysis. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 29-43 8621717-1 1996 We have recently described an ATP-driven, valinomycin-dependent 86Rb+uptake into proteoliposomes reconstituted with mammalian P-glycoprotein (Eytan, G. D., Borgnia, M. J., Regev, R., and Assaraf, Y. G. (1994) J. Biol. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 126-140 8621717-4 1996 P-glycoprotein mediated the ATP-dependent uptake of 86Rb+-ionophore complex into the proteoliposomes, where the radioactive cation was accumulated, thus, circumventing the obstacle posed by the hydrophobicity of P-glycoprotein substrates in transport studies. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 8621717-4 1996 P-glycoprotein mediated the ATP-dependent uptake of 86Rb+-ionophore complex into the proteoliposomes, where the radioactive cation was accumulated, thus, circumventing the obstacle posed by the hydrophobicity of P-glycoprotein substrates in transport studies. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 212-226 8621717-5 1996 Taking advantage of this assay and of the high levels of P-glycoprotein expression in multidrug-resistant Chinese hamster ovary cells, we measured simultaneously both the ATPase and transport activities of P-glycoprotein under identical conditions and observed 0.5-0.8 ionophore molecules transported/ATP molecule hydrolyzed. Adenosine Triphosphate 171-174 ATP binding cassette subfamily B member 1 Homo sapiens 206-220 8621717-6 1996 The amount of 86Rb+ ions transported within 1 min via the ATP- and valinomycin-dependent P-glycoprotein was equivalent to an intravesicular cation concentration of 8 mM. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 89-103 8621717-8 1996 This constitutes the first demonstration of comparable rates of P-glycoprotein-catalyzed substrate transport and ATP hydrolysis. Adenosine Triphosphate 113-116 ATP binding cassette subfamily B member 1 Homo sapiens 64-78 7473231-2 1995 P-glycoprotein, the protein product of the multidrug resistance (MDR1) gene, has ATP-dependent transporter activity. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 65-69 7608182-2 1995 Although P-glycoprotein is widely believed to function as an ATP-dependent drug efflux pump, the unusually broad substrate specificity of P-glycoprotein has engendered the proposal of other, less direct mechanisms. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 9-23 7608182-2 1995 Although P-glycoprotein is widely believed to function as an ATP-dependent drug efflux pump, the unusually broad substrate specificity of P-glycoprotein has engendered the proposal of other, less direct mechanisms. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 138-152 7790125-11 1995 Dissociated, viable XG cells appeared to have ATP levels sufficient to sustain Pgp-ATPase-coupled drug transport. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 79-82 7790125-17 1995 Also our results indicate that Pgp activity may be impaired in vivo at [ATP]i below 2 mM, which are realistic values for human solid tumors. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 31-34 8567633-1 1996 The characteristics of P-glycoprotein (MDR1), an ATP-dependent drug extrusion pump responsible for the multidrug resistance of human cancer, were investigated in an in vitro expression system. Adenosine Triphosphate 49-52 ATP binding cassette subfamily B member 1 Homo sapiens 39-43 8549739-1 1995 P-glycoprotein is a plasma-membrane glycoprotein which confers multidrug-resistance on cells and displays ATP-driven drug-pumping in vitro. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 7645952-2 1995 P-gp acts as an ATP-dependent efflux pump causing a decreased intracellular accumulation of structurally unrelated natural anticancer agents such as anthracyclines. Adenosine Triphosphate 16-19 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 7730340-1 1995 P-glycoprotein functions as an ATP-driven active efflux pump for many cytotoxic drugs. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 7730340-6 1995 Peptides and ionophores also effectively inhibited ATP-dependent drug transport by P-glycoprotein in plasma membrane vesicles. Adenosine Triphosphate 51-54 ATP binding cassette subfamily B member 1 Homo sapiens 83-97 7629047-2 1995 P-glycoprotein, a member of the ATP-binding cassette (ABC) superfamily of transporters, is believed to function as an ATP-dependent drug efflux pump with broad specificity for chemically unrelated hydrophobic compounds. Adenosine Triphosphate 32-35 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 7629050-1 1995 The human multidrug resistance protein, or P-glycoprotein (Pgp), exhibits a high-capacity drug-dependent ATP hydrolytic activity that is a direct reflection of its drug transport capability. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 43-57 7629050-1 1995 The human multidrug resistance protein, or P-glycoprotein (Pgp), exhibits a high-capacity drug-dependent ATP hydrolytic activity that is a direct reflection of its drug transport capability. Adenosine Triphosphate 105-108 ATP binding cassette subfamily B member 1 Homo sapiens 59-62 7629050-5 1995 Many of the drugs stimulate the Pgp ATPase activity, but certain drugs bind tightly to the drug-binding site of the Pgp without eliciting ATP hydrolysis. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 32-35 7629050-5 1995 Many of the drugs stimulate the Pgp ATPase activity, but certain drugs bind tightly to the drug-binding site of the Pgp without eliciting ATP hydrolysis. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 116-119 7559741-8 1995 Furthermore, though its activity was reduced, the reconstituted P-glycoprotein was shown to be an ATP-dependent transporter of vincristine. Adenosine Triphosphate 98-101 ATP binding cassette subfamily B member 1 Homo sapiens 64-78 7912280-6 1994 These data indicate that P-glycoprotein, which is normally energized by ATP hydrolysis, is incapable of catalyzing organic cation/proton exchange or organic cation/organic cation exchange, properties of the organic cation carrier of renal proximal tubule BBMV. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 25-39 7932810-3 1994 This drug pump is composed of a 170-kd transmembrane glycoprotein referred to as the P-glycoprotein (P-gp) that uses energy in the form of adenosine triphosphate to transport drugs through a channel formed by transmembrane segments. Adenosine Triphosphate 139-161 ATP binding cassette subfamily B member 1 Homo sapiens 85-99 7932810-3 1994 This drug pump is composed of a 170-kd transmembrane glycoprotein referred to as the P-glycoprotein (P-gp) that uses energy in the form of adenosine triphosphate to transport drugs through a channel formed by transmembrane segments. Adenosine Triphosphate 139-161 ATP binding cassette subfamily B member 1 Homo sapiens 101-105 7706385-1 1994 P-glycoprotein (P-gp), the product of the human multidrug resistance (MDR1) gene, confers multidrug resistance on cells by acting as an ATP-dependent drug transporter. Adenosine Triphosphate 136-139 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 7706385-1 1994 P-glycoprotein (P-gp), the product of the human multidrug resistance (MDR1) gene, confers multidrug resistance on cells by acting as an ATP-dependent drug transporter. Adenosine Triphosphate 136-139 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 7706385-1 1994 P-glycoprotein (P-gp), the product of the human multidrug resistance (MDR1) gene, confers multidrug resistance on cells by acting as an ATP-dependent drug transporter. Adenosine Triphosphate 136-139 ATP binding cassette subfamily B member 1 Homo sapiens 70-74 7913830-1 1994 We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 132-146 7913830-1 1994 We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 148-151 15275432-1 1994 The P-glycoprotein (Pgp), a member of a group of integral membrane proteins that contain the ATP-binding cassette, is widely represented in the animal kingdom. Adenosine Triphosphate 93-96 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 15275432-1 1994 The P-glycoprotein (Pgp), a member of a group of integral membrane proteins that contain the ATP-binding cassette, is widely represented in the animal kingdom. Adenosine Triphosphate 93-96 ATP binding cassette subfamily B member 1 Homo sapiens 20-23 7909435-1 1994 P-glycoprotein functions as an ATP-dependent pump for a diverse spectrum of compounds. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 7911330-3 1994 Evidence suggesting involvement of P-Glycoprotein (P-Gp), included (1) comparable transport of P-Gp substrate doxorubicin, (2) transport stimulation by ATP and (3) transport suppression by the P-Gp inhibitor, verapamil. Adenosine Triphosphate 152-155 ATP binding cassette subfamily B member 1 Homo sapiens 35-49 8100632-6 1993 The limitation of P-glycoprotein substrate absorption by active ATP-dependent export via P-glycoprotein is discussed, together with the possibility that other classes of substrate may be substrates for different ATP-dependent export pumps. Adenosine Triphosphate 64-67 ATP binding cassette subfamily B member 1 Homo sapiens 18-32 7905747-1 1994 Multidrug resistant cancer cells of the MDR-1 phenotype utilize an ATP-dependent pump to excrete toxic drugs. Adenosine Triphosphate 67-70 ATP binding cassette subfamily B member 1 Homo sapiens 40-45 7901214-1 1993 P-glycoprotein, the multidrug transporter, is proposed to act as an ATP-driven drug efflux pump. Adenosine Triphosphate 68-71 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 8098711-8 1993 These data suggest that a portion of the drug binding site in P-glycoprotein is in close proximity to ATP binding regions. Adenosine Triphosphate 102-105 ATP binding cassette subfamily B member 1 Homo sapiens 62-76 7913667-2 1994 P-glycoprotein (Pgp) is an ATP-dependent drug efflux pump responsible for classical multi-drug resistance (MDR). Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 7913667-2 1994 P-glycoprotein (Pgp) is an ATP-dependent drug efflux pump responsible for classical multi-drug resistance (MDR). Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 8100632-6 1993 The limitation of P-glycoprotein substrate absorption by active ATP-dependent export via P-glycoprotein is discussed, together with the possibility that other classes of substrate may be substrates for different ATP-dependent export pumps. Adenosine Triphosphate 64-67 ATP binding cassette subfamily B member 1 Homo sapiens 89-103 8100632-6 1993 The limitation of P-glycoprotein substrate absorption by active ATP-dependent export via P-glycoprotein is discussed, together with the possibility that other classes of substrate may be substrates for different ATP-dependent export pumps. Adenosine Triphosphate 212-215 ATP binding cassette subfamily B member 1 Homo sapiens 18-32 8095827-3 1993 P-glycoprotein is an integral plasma membrane protein which contains 12 putative transmembrane regions and two ATP binding sites. Adenosine Triphosphate 111-114 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 8094997-9 1993 Pgp-mediated [99mTc]SESTAMIBI transport occurred against its electrochemical gradient and was found to be ATP dependent displaying an apparent Km of 50 microM. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 8094292-2 1993 We now show evidence that cell lines with a high expression of Pgp display a reduced accumulation of cortisol and an ATP-dependent outward transport of the hormone. Adenosine Triphosphate 117-120 ATP binding cassette subfamily B member 1 Homo sapiens 63-66 7765321-4 1993 The P-glycoprotein is a member of a large superfamily of transport proteins which utilize ATP to translocate a wide range of substrates across biological membranes. Adenosine Triphosphate 90-93 ATP binding cassette subfamily B member 1 Homo sapiens 4-18 8093688-1 1993 The surfactant Cremophor EL has recently been shown to reverse the multiple drug resistance phenotype (mdr1) created by over-expression of P glycoprotein, an ATP dependent membrane transport system. Adenosine Triphosphate 158-161 ATP binding cassette subfamily B member 1 Homo sapiens 103-107 8093688-1 1993 The surfactant Cremophor EL has recently been shown to reverse the multiple drug resistance phenotype (mdr1) created by over-expression of P glycoprotein, an ATP dependent membrane transport system. Adenosine Triphosphate 158-161 ATP binding cassette subfamily B member 1 Homo sapiens 139-153 8094005-6 1993 Inhibition of PGP function by depletion of ATP increased influx by 6.8-fold and 2.2-fold in the two cell types, respectively. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 14-17 8094005-7 1993 Mutation of PGP at both ATP binding sites abolished its ability to limit initial influx. Adenosine Triphosphate 24-27 ATP binding cassette subfamily B member 1 Homo sapiens 12-15 8093856-2 1993 MDR in tumor cells is associated with the over-expression of P-glycoprotein, an ATP-dependent cell-membrane transport molecule. Adenosine Triphosphate 80-83 ATP binding cassette subfamily B member 1 Homo sapiens 61-75 7765326-5 1993 P-glycoprotein functions as an ATP-dependent, unidirectional drug efflux pump with a broad substrate specificity, that effectively maintains the intracellular cytotoxic drug concentrations under a non-cytotoxic threshold value. Adenosine Triphosphate 31-34 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 7678345-1 1993 The multidrug resistance (mdr1) gene product, P-glycoprotein, is responsible for the ATP-dependent extrusion of a variety of compounds, including chemotherapeutic drugs, from cells. Adenosine Triphosphate 85-88 ATP binding cassette subfamily B member 1 Homo sapiens 26-30 7678345-1 1993 The multidrug resistance (mdr1) gene product, P-glycoprotein, is responsible for the ATP-dependent extrusion of a variety of compounds, including chemotherapeutic drugs, from cells. Adenosine Triphosphate 85-88 ATP binding cassette subfamily B member 1 Homo sapiens 46-60 7678345-2 1993 The data presented here show that cells with increased levels of the P-glycoprotein release ATP to the medium in proportion to the concentration of the protein in their plasma membrane. Adenosine Triphosphate 92-95 ATP binding cassette subfamily B member 1 Homo sapiens 69-83 1358431-7 1992 However, a polyclonal antibody directed against a synthetic peptide corresponding to the putative ATP binding domain of P-glycoprotein reacted with two (M(r) 42,000 and 85,000) membrane proteins from MCF/MX cells which were not found in MCF/WT. Adenosine Triphosphate 98-101 ATP binding cassette subfamily B member 1 Homo sapiens 120-134 1359408-3 1992 Within each ameba P-glycoprotein were the ATP-binding sites found twice in eukaryotic P-glycoproteins and once in prokaryotic transport proteins. Adenosine Triphosphate 42-45 ATP binding cassette subfamily B member 1 Homo sapiens 18-32 1356264-1 1992 Multidrug-resistant human tumor cells overexpress the MDR1 gene product P-glycoprotein, which is believed to function as an ATP-dependent efflux pump. Adenosine Triphosphate 124-127 ATP binding cassette subfamily B member 1 Homo sapiens 54-58 1356264-1 1992 Multidrug-resistant human tumor cells overexpress the MDR1 gene product P-glycoprotein, which is believed to function as an ATP-dependent efflux pump. Adenosine Triphosphate 124-127 ATP binding cassette subfamily B member 1 Homo sapiens 72-86 1356264-2 1992 In this study we demonstrate that the partially purified P-glycoprotein, when reconstituted in an artificial membrane, catalyzes drug-stimulated ATP hydrolysis. Adenosine Triphosphate 145-148 ATP binding cassette subfamily B member 1 Homo sapiens 57-71 1356264-10 1992 These data indicate that the P-glycoprotein, similar to other ion-transporting ATPases, exhibits a high level of ATP hydrolysis (5-12 mumol per min per mg of protein). Adenosine Triphosphate 79-82 ATP binding cassette subfamily B member 1 Homo sapiens 29-43 1359408-4 1992 Hydropathy plots of the ameba P-glycoproteins were nearly superimposable on that of the human mdr 1, showing 2 homologous halves, each containing an ATP-binding site and 6 hydrophobic transmembrane domains that form the putative channel. Adenosine Triphosphate 149-152 ATP binding cassette subfamily B member 1 Homo sapiens 94-99 1359408-6 1992 Also identified in the E. histolytica genomic library were 2 P-glycoprotein pseudogenes, each with a frame shift and stop codons in identical places within the amino ATP-binding site. Adenosine Triphosphate 166-169 ATP binding cassette subfamily B member 1 Homo sapiens 61-75 1680871-0 1991 Modulation of ATP and drug binding by monoclonal antibodies against P-glycoprotein. Adenosine Triphosphate 14-17 ATP binding cassette subfamily B member 1 Homo sapiens 68-82 1347041-4 1992 Immunoprecipitated full-length P-glycoprotein beta-galactosidase showed ATPase activity with apparent specific activity of 180 nmol/mg/min, a value higher than previously reported, in the presence of phospholipids, suggesting that stabilization of the transmembrane domains is necessary for ATP hydrolysis. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 31-45 1347041-5 1992 N-terminal half P-glycoprotein-beta-galactosidase also showed ability to hydrolyze ATP but with slightly lower specific activity. Adenosine Triphosphate 83-86 ATP binding cassette subfamily B member 1 Homo sapiens 16-30 1375156-1 1992 P-glycoprotein (MDR1), that confers multidrug resistance in cancer, and the cystic-fibrosis transmembrane-conductance regulator (CFTR), that is causative defective in cystic fibrosis, belong to the family of ATP-binding transport proteins. Adenosine Triphosphate 208-211 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 1356900-1 1992 P-glycoprotein, the product of the multidrug resistance (MDR1) gene, is an ATP-driven transmembrane pump that increases the resistance of cells by actively exporting toxic chemicals. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 1356900-1 1992 P-glycoprotein, the product of the multidrug resistance (MDR1) gene, is an ATP-driven transmembrane pump that increases the resistance of cells by actively exporting toxic chemicals. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 57-61 1347041-2 1992 Two ATP-binding domains are found in members of the family of ATP-dependent transport proteins, which includes P-glycoprotein and cystic fibrosis transmembrane conductance regulator. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 111-125 1347041-2 1992 Two ATP-binding domains are found in members of the family of ATP-dependent transport proteins, which includes P-glycoprotein and cystic fibrosis transmembrane conductance regulator. Adenosine Triphosphate 62-65 ATP binding cassette subfamily B member 1 Homo sapiens 111-125 1347041-3 1992 To investigate the involvement of the two ATP-binding domains in the ATPase activity of P-glycoprotein, full-length and the 5"-half of human MDR1 cDNA, which encodes P-glycoprotein, were fused with the Escherichia coli lacZ gene and expressed in NIH3T3 cells. Adenosine Triphosphate 42-45 ATP binding cassette subfamily B member 1 Homo sapiens 88-102 1347041-3 1992 To investigate the involvement of the two ATP-binding domains in the ATPase activity of P-glycoprotein, full-length and the 5"-half of human MDR1 cDNA, which encodes P-glycoprotein, were fused with the Escherichia coli lacZ gene and expressed in NIH3T3 cells. Adenosine Triphosphate 42-45 ATP binding cassette subfamily B member 1 Homo sapiens 141-145 1347031-3 1992 Jejunal and ileal brush border membrane vesicles, but not basolateral membrane vesicles, manifested adenosine triphosphate (ATP)-dependent transport of daunomycin, a substrate for Gp170, and contained a approximately 170-kilodalton protein that reacts with anti-Gp170 monoclonal antibody. Adenosine Triphosphate 100-122 ATP binding cassette subfamily B member 1 Homo sapiens 180-185 1347031-3 1992 Jejunal and ileal brush border membrane vesicles, but not basolateral membrane vesicles, manifested adenosine triphosphate (ATP)-dependent transport of daunomycin, a substrate for Gp170, and contained a approximately 170-kilodalton protein that reacts with anti-Gp170 monoclonal antibody. Adenosine Triphosphate 124-127 ATP binding cassette subfamily B member 1 Homo sapiens 180-185 1347031-5 1992 ATP-dependent daunomycin transport by brush border vesicles was unidirectional (inside to outside) and temperature dependent and was blocked by Gp170 inhibitors but not by taurocholate or bromsulphalein glutathione. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 144-149 1347031-7 1992 These results suggest that Gp170 in rat small intestinal brush border membrane vesicles is an ATP-dependent efflux pump responsible for the transport of Gp170 substrates into the small intestinal lumen. Adenosine Triphosphate 94-97 ATP binding cassette subfamily B member 1 Homo sapiens 27-32 1347031-7 1992 These results suggest that Gp170 in rat small intestinal brush border membrane vesicles is an ATP-dependent efflux pump responsible for the transport of Gp170 substrates into the small intestinal lumen. Adenosine Triphosphate 94-97 ATP binding cassette subfamily B member 1 Homo sapiens 153-158 1364101-3 1992 Within each ameba P-glycoprotein were the ATP-binding sites found twice in eukaryotic P-glycoproteins and once in prokaryotic transport proteins. Adenosine Triphosphate 42-45 ATP binding cassette subfamily B member 1 Homo sapiens 18-32 1364101-5 1992 In addition, there were two P-glycoprotein pseudogenes, each with a frame shift and stop codons in identical places within the amino ATP-binding site. Adenosine Triphosphate 133-136 ATP binding cassette subfamily B member 1 Homo sapiens 28-42 1682809-1 1991 BACKGROUND AND METHODS: Multidrug resistance in chemotherapy for cancer is characterized by increased genetic expression of P-glycoprotein, which acts as an ATP-dependent drug-efflux pump. Adenosine Triphosphate 157-160 ATP binding cassette subfamily B member 1 Homo sapiens 124-138 1911388-2 1991 This gene codes for P-glycoprotein, a proposed ATP-dependent drug efflux pump, which reduces the net intracellular accumulation of a large group of chemotherapeutic agents in resistant cells. Adenosine Triphosphate 47-50 ATP binding cassette subfamily B member 1 Homo sapiens 20-34 1680871-3 1991 Structurally, P-glycoprotein is an internally duplicated molecule containing two large multi-spanning transmembrane domains and two cytoplasmic ATP binding domains. Adenosine Triphosphate 144-147 ATP binding cassette subfamily B member 1 Homo sapiens 14-28 1680871-4 1991 In this report we demonstrate that monoclonal antibodies C219, C494, and C32 directed against short linear regions of the P-glycoprotein molecule inhibit ATP binding to P-glycoprotein in vitro. Adenosine Triphosphate 154-157 ATP binding cassette subfamily B member 1 Homo sapiens 122-136 1680871-4 1991 In this report we demonstrate that monoclonal antibodies C219, C494, and C32 directed against short linear regions of the P-glycoprotein molecule inhibit ATP binding to P-glycoprotein in vitro. Adenosine Triphosphate 154-157 ATP binding cassette subfamily B member 1 Homo sapiens 169-183 1680871-7 1991 These observations are the first evidence linking specific perturbations of the P-glycoprotein molecule with ATP and drug binding. Adenosine Triphosphate 109-112 ATP binding cassette subfamily B member 1 Homo sapiens 80-94 1977744-2 1990 One type of multidrug resistance is characterized by the decreased accumulation of hydrophobic natural product drugs, a phenotype which is mediated by an ATP-dependent integral membrane multidrug transporter termed P-glycoprotein or P170. Adenosine Triphosphate 154-157 ATP binding cassette subfamily B member 1 Homo sapiens 215-229 1671863-3 1991 A domain by domain comparison of this protein with p-glycoproteins capable of supporting multidrug resistance, i.e. human mdr1, mouse mdr1/mdr1b, and mouse mdr3/mdr1a, shows that, in addition to the ATP binding sites, the second, fourth, and eleventh transmembrane domains and the four small intracellular loops, IC-1, IC-2, IC-4, and IC-5, are highly conserved and are therefore likely to be important for the maintenance of p-glycoprotein function. Adenosine Triphosphate 199-202 ATP binding cassette subfamily B member 1 Homo sapiens 122-126 1671642-1 1991 Resistance of human cancer cells to multiple cytotoxic hydrophobic agents (multidrug resistance) is due to overexpression of the MDR1 gene whose product is the ATP-dependent multidrug transporter, P-glycoprotein. Adenosine Triphosphate 160-163 ATP binding cassette subfamily B member 1 Homo sapiens 129-133 1970935-1 1990 The plasma membrane associated human multidrug resistance (MDR1) gene product, known as the 170-kDa P-glycoprotein or the multidrug transporter, acts as an ATP-dependent efflux pump for various cytotoxic agents. Adenosine Triphosphate 156-159 ATP binding cassette subfamily B member 1 Homo sapiens 59-63 1970935-1 1990 The plasma membrane associated human multidrug resistance (MDR1) gene product, known as the 170-kDa P-glycoprotein or the multidrug transporter, acts as an ATP-dependent efflux pump for various cytotoxic agents. Adenosine Triphosphate 156-159 ATP binding cassette subfamily B member 1 Homo sapiens 100-114 30052463-9 2018 In addition, the compound activated the ABCB1-mediated ATP hydrolysis and inhibited the photolabeling of ABCB1 with [125I]-iodoarylazidoprazosin. Adenosine Triphosphate 55-58 ATP binding cassette subfamily B member 1 Homo sapiens 40-45 2116181-5 1990 HlyB is a member of a family of membrane proteins engaged in ATP dependent secretion mechanisms conserved in many organisms including man (P-glycoprotein and the CF protein). Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 139-153 33350827-1 2021 P-Glycoprotein (P-gp) is an ATP-dependent efflux pump that clears a wide variety of drugs and toxins from cells. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 33350827-1 2021 P-Glycoprotein (P-gp) is an ATP-dependent efflux pump that clears a wide variety of drugs and toxins from cells. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 33031417-6 2020 Mutation of the catalytic glutamate in ABCB1 results in the occlusion of ATP in NBS1, leading to the arrest of the transport cycle. Adenosine Triphosphate 73-76 ATP binding cassette subfamily B member 1 Homo sapiens 39-44 33031417-7 2020 Here we show that despite the catalytic glutamate mutation (E556M), ABCB1 regains its ATP-dependent transport activity, when three additional diverging residues are also replaced. Adenosine Triphosphate 86-89 ATP binding cassette subfamily B member 1 Homo sapiens 68-73 33031417-9 2020 In summary, we show that ABCB1 can be transformed into an active transporter with only one functional catalytic site by preventing the formation of the ATP-locked pre-hydrolytic state in the non-canonical site. Adenosine Triphosphate 152-155 ATP binding cassette subfamily B member 1 Homo sapiens 25-30 30052463-9 2018 In addition, the compound activated the ABCB1-mediated ATP hydrolysis and inhibited the photolabeling of ABCB1 with [125I]-iodoarylazidoprazosin. Adenosine Triphosphate 55-58 ATP binding cassette subfamily B member 1 Homo sapiens 105-110 18480151-0 2008 Influence of adenosine triphosphate and ABCB1 (MDR1) genotype on the P-glycoprotein-dependent transfer of saquinavir in the dually perfused human placenta. Adenosine Triphosphate 13-35 ATP binding cassette subfamily B member 1 Homo sapiens 69-83 30463238-6 2018 Further mechanistic studies showed that PHP micelles were able to inhibit P-glycoprotein (P-gp) activity by lowering mitochondrial membrane potentials and ATP levels. Adenosine Triphosphate 155-158 ATP binding cassette subfamily B member 1 Homo sapiens 74-88 30463238-6 2018 Further mechanistic studies showed that PHP micelles were able to inhibit P-glycoprotein (P-gp) activity by lowering mitochondrial membrane potentials and ATP levels. Adenosine Triphosphate 155-158 ATP binding cassette subfamily B member 1 Homo sapiens 90-94 18480151-1 2008 BACKGROUND: The ATP-dependent drug-efflux pump, P-glycoprotein (P-gp) encoded by ABCB1 (MDR1), plays a crucial role in several tissues forming blood-tissue barriers. Adenosine Triphosphate 16-19 ATP binding cassette subfamily B member 1 Homo sapiens 48-62 18480151-1 2008 BACKGROUND: The ATP-dependent drug-efflux pump, P-glycoprotein (P-gp) encoded by ABCB1 (MDR1), plays a crucial role in several tissues forming blood-tissue barriers. Adenosine Triphosphate 16-19 ATP binding cassette subfamily B member 1 Homo sapiens 64-68 18480151-1 2008 BACKGROUND: The ATP-dependent drug-efflux pump, P-glycoprotein (P-gp) encoded by ABCB1 (MDR1), plays a crucial role in several tissues forming blood-tissue barriers. Adenosine Triphosphate 16-19 ATP binding cassette subfamily B member 1 Homo sapiens 81-86 18480151-1 2008 BACKGROUND: The ATP-dependent drug-efflux pump, P-glycoprotein (P-gp) encoded by ABCB1 (MDR1), plays a crucial role in several tissues forming blood-tissue barriers. Adenosine Triphosphate 16-19 ATP binding cassette subfamily B member 1 Homo sapiens 88-92 18480151-3 2008 METHODS: We have studied the dose-response effect of exogenous ATP on the placental transfer of the well-established P-gp substrate saquinavir in 17 dually perfused human term placentas. Adenosine Triphosphate 63-66 ATP binding cassette subfamily B member 1 Homo sapiens 117-121 35634783-2 2022 Structures of ABCB1 provided insights into the structural changes that occur upon ATP binding and the characteristic architecture of the substrate binding site. Adenosine Triphosphate 82-85 ATP binding cassette subfamily B member 1 Homo sapiens 14-19 34624510-4 2022 Furthermore, ATP depletion impairs P-gp activity to enhance the antitumor activity of DOX. Adenosine Triphosphate 13-16 ATP binding cassette subfamily B member 1 Homo sapiens 35-39 34775061-3 2022 The P-gp overexpression in cancer cells depends on several factors like adenosine triphosphate (ATP) hydrolysis, hypoxia-inducible factor 1 alpha (HIF-1alpha), and drug physicochemical properties such as lipophilicity, molecular weight, and molecular size. Adenosine Triphosphate 96-99 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 34418731-1 2021 P-glycoprotein (P-gp) is an adenosine triphosphate (ATP)-dependent drug efflux protein commonly associated with multidrug resistance in cancer chemotherapy. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 34418731-1 2021 P-glycoprotein (P-gp) is an adenosine triphosphate (ATP)-dependent drug efflux protein commonly associated with multidrug resistance in cancer chemotherapy. Adenosine Triphosphate 52-55 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 34907862-7 2021 Few well-characterized AAs were screened for their drug-likeness properties and tested for binding affinity at ATP and drug binding sites of ABCB1 through molecular docking. Adenosine Triphosphate 111-114 ATP binding cassette subfamily B member 1 Homo sapiens 141-146 34907862-10 2021 MD simulation of Annonacin A: ABCB1 complex for 100 ns also indicated that Annonacin A would stably bind to the ATP binding site. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 30-35 34533937-6 2021 Intriguingly, the PDT-induced in situ mitochondrial dysfunction led to intracellular ATP reduction, which triggered the downregulated P-glycoprotein transportation capacity and thus resulted in diminishing the efflux of chemotherapeutic agents and increasing drug uptake by drug-resistant cells. Adenosine Triphosphate 85-88 ATP binding cassette subfamily B member 1 Homo sapiens 134-148 34084427-2 2021 Allosteric modulation by various ligands (e.g., transport substrates, inhibitors, and ATP) has been biochemically shown to directly influence structural dynamics, and thereby, the function of Pgp. Adenosine Triphosphate 86-89 ATP binding cassette subfamily B member 1 Homo sapiens 192-195 33735724-6 2021 ATPase assay and MDR1 shift assay were adopted to reveal the ATP consumption and conformational change of P-gp. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 106-110 35471238-2 2022 Recent developments in the studies of drug resistance have identified compounds such as verapamil and tamoxifen that specifically target ABCB1-expressing multidrug resistant (MDR) cells, through an ATP-dependent ROS-generating mechanism. Adenosine Triphosphate 198-201 ATP binding cassette subfamily B member 1 Homo sapiens 137-142 35401832-6 2022 It has the potential to enhance chemosensitivity by downregulating of P-gp expression due to the increased production of ATP-consuming chaperones. Adenosine Triphosphate 121-124 ATP binding cassette subfamily B member 1 Homo sapiens 70-74 35075112-4 2022 HCC has evolved different mechanisms of resistance to chemotherapy including those involving the P-glycoprotein (P-gp), an ATP-dependent drug efflux pump, which controls intracellular drug concentration. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 97-111 35075112-4 2022 HCC has evolved different mechanisms of resistance to chemotherapy including those involving the P-glycoprotein (P-gp), an ATP-dependent drug efflux pump, which controls intracellular drug concentration. Adenosine Triphosphate 123-126 ATP binding cassette subfamily B member 1 Homo sapiens 113-117 2571333-4 1989 Furthermore, an increase in ATP enhanced VCR binding in proportion to content of the MDR associated P-glycoprotein was seen in plasma membrane vesicles prepared from various benign human endocrine tumors. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 100-114 2565823-7 1989 Verapamil induced an extra amount of ATP consumption in P-glycoprotein-expressing 2780AD cells of approx. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 56-70 2900677-5 1988 GTP was also hydrolyzed by the P-glycoprotein, although less efficiently than ATP. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 31-45 32229058-17 2021 P-gp-mediated drug efflux was ATP-dependent. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 32229058-20 2021 P-gp dysfunction induces mitochondrial stress to generate ATP. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 34011375-5 2021 With near infrared (NIR)-irradiation, PTX@GO-PEG-OSA could generate excessive reactive oxygen species (ROS), attack mitochondrial respiratory chain complex enzyme, reduce adenosine-triphosphate (ATP) supplement for P-gp, and effectively inhibit P-gp"s efflux pump function. Adenosine Triphosphate 195-198 ATP binding cassette subfamily B member 1 Homo sapiens 215-219 34011375-5 2021 With near infrared (NIR)-irradiation, PTX@GO-PEG-OSA could generate excessive reactive oxygen species (ROS), attack mitochondrial respiratory chain complex enzyme, reduce adenosine-triphosphate (ATP) supplement for P-gp, and effectively inhibit P-gp"s efflux pump function. Adenosine Triphosphate 195-198 ATP binding cassette subfamily B member 1 Homo sapiens 245-249 33965440-1 2021 BACKGROUND: P-glycoprotein (P-gp) is a prevalent resistance mediator and it requires considerable cellular energy to ensure ATP dependent efflux of anticancer drugs. Adenosine Triphosphate 124-127 ATP binding cassette subfamily B member 1 Homo sapiens 12-26 33965440-1 2021 BACKGROUND: P-glycoprotein (P-gp) is a prevalent resistance mediator and it requires considerable cellular energy to ensure ATP dependent efflux of anticancer drugs. Adenosine Triphosphate 124-127 ATP binding cassette subfamily B member 1 Homo sapiens 28-32 33965440-2 2021 The glycolytic pathway generates the majority of catabolic energy in cancer cells; however, the high rates of P-gp activity places added strain on its inherently limited capacity to generate ATP. Adenosine Triphosphate 191-194 ATP binding cassette subfamily B member 1 Homo sapiens 110-114 33965440-3 2021 This is particularly relevant for compounds such as verapamil that are believed to trap P-gp in a futile transport process that requires continuing ATP consumption. Adenosine Triphosphate 148-151 ATP binding cassette subfamily B member 1 Homo sapiens 88-92 33965440-6 2021 Even in the absence of verapamil, P-gp expressing cells display near maximal rates of glycolysis and oxidative phosphorylation, which prevents an adequate response to the demand for ATP to sustain transport activity. Adenosine Triphosphate 182-185 ATP binding cassette subfamily B member 1 Homo sapiens 34-38 33965440-8 2021 CONCLUSIONS: Our results demonstrate that the strained metabolic profiles of P-gp expressing resistant cancer cells can be overwhelmed by additional ATP demands. Adenosine Triphosphate 149-152 ATP binding cassette subfamily B member 1 Homo sapiens 77-81 35601326-1 2022 P-glycoprotein (Pgp), an ATP binding cassette (ABC) transporter, is an ATP-dependent efflux pump responsible for cancer multidrug resistance. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 35601326-1 2022 P-glycoprotein (Pgp), an ATP binding cassette (ABC) transporter, is an ATP-dependent efflux pump responsible for cancer multidrug resistance. Adenosine Triphosphate 71-74 ATP binding cassette subfamily B member 1 Homo sapiens 16-19 35044379-8 2022 Moreover, alpha-TOS also consumed adenosine triphosphate (ATP) to impair ATP-dependent P-gp mediated drug efflux to reverse the tumor"s drug resistance. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 35044379-8 2022 Moreover, alpha-TOS also consumed adenosine triphosphate (ATP) to impair ATP-dependent P-gp mediated drug efflux to reverse the tumor"s drug resistance. Adenosine Triphosphate 73-76 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 35215316-3 2022 The PfMDR1 transporter (or P-glycoprotein 1) is located in the membrane of the digestive vacuole (DV), functions as an ATP-dependent pump, and transports substrates into the DV. Adenosine Triphosphate 119-122 ATP binding cassette subfamily B member 1 Homo sapiens 27-43 35103340-2 2022 It is situated on the surface of the intestine epithelium, where P-gp substrate binds to the transporter and is pumped into the intestine lumen by the ATP-driven energy-dependent process. Adenosine Triphosphate 151-154 ATP binding cassette subfamily B member 1 Homo sapiens 65-69 2568355-4 1989 Canalicular membrane vesicles (CMV), but not sinusoidal membrane vesicles, contained a approximately 160-kDa protein which reacts with anti-Gp170 monoclonal antibody and manifest ATP-dependent [3H]daunomycin transport which is temperature dependent, osmotically sensitive, and saturable. Adenosine Triphosphate 179-182 ATP binding cassette subfamily B member 1 Homo sapiens 140-145 2568355-9 1989 These results suggest that Gp170 is an ATP-dependent efflux pump which is responsible for the undirectional, energy-dependent transport of daunomycin and other drugs by rat liver into the bile. Adenosine Triphosphate 39-42 ATP binding cassette subfamily B member 1 Homo sapiens 27-32 2886389-0 1987 ATP-binding properties of P glycoprotein from multidrug-resistant KB cells. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 26-40 2878368-6 1986 The C-terminal region of P-glycoprotein and the HlyB protein contain sequences homologous to the nucleotide-binding domains of a group of closely related bacterial ATP-binding proteins. Adenosine Triphosphate 164-167 ATP binding cassette subfamily B member 1 Homo sapiens 25-39 34000577-6 2021 Further mechanistic studies indicated that Cur inhibited the ATP-dependent transport activity of P-gp, thereby increasing the intra-celluar accumulation of Dox in drug-resistant cells. Adenosine Triphosphate 61-64 ATP binding cassette subfamily B member 1 Homo sapiens 97-101 33967805-6 2021 Multidrug and toxin extrusion protein 1 (MATE1, gene symbol: SLC47A1) and the ATP-driven efflux pump P-glycoprotein (P-gp, gene symbol: ABCB1) are both expressed in the canalicular membrane of human hepatocytes and are described as transporters of organic cations. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 101-115 33901197-6 2021 We also showed that Abeta42 stimulated the ATP hydrolysis activity of isolated P-gp in nanodiscs. Adenosine Triphosphate 43-46 ATP binding cassette subfamily B member 1 Homo sapiens 79-83 33967805-6 2021 Multidrug and toxin extrusion protein 1 (MATE1, gene symbol: SLC47A1) and the ATP-driven efflux pump P-glycoprotein (P-gp, gene symbol: ABCB1) are both expressed in the canalicular membrane of human hepatocytes and are described as transporters of organic cations. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 117-121 33967805-6 2021 Multidrug and toxin extrusion protein 1 (MATE1, gene symbol: SLC47A1) and the ATP-driven efflux pump P-glycoprotein (P-gp, gene symbol: ABCB1) are both expressed in the canalicular membrane of human hepatocytes and are described as transporters of organic cations. Adenosine Triphosphate 78-81 ATP binding cassette subfamily B member 1 Homo sapiens 136-141 32706272-7 2020 In addition, the generated ROS promoted the release of PTX from DEX-TK-PTX to kill cancer cells, while ATP depletion inhibited P-gp-mediated MDR. Adenosine Triphosphate 103-106 ATP binding cassette subfamily B member 1 Homo sapiens 127-131 33159693-0 2021 Conformational changes of the nucleotide binding domains of P-glycoprotein induced by ATP hydrolysis. Adenosine Triphosphate 86-89 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 33159693-2 2021 Pgp nucleotide binding domains (NBDs) drive the transport cycle through ATP binding and hydrolysis. Adenosine Triphosphate 72-75 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 33275773-0 2021 ABCB1/MDR1/P-gp employs an ATP-dependent twist-and-squeeze mechanism to export hydrophobic drugs. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 0-5 33275773-0 2021 ABCB1/MDR1/P-gp employs an ATP-dependent twist-and-squeeze mechanism to export hydrophobic drugs. Adenosine Triphosphate 27-30 ATP binding cassette subfamily B member 1 Homo sapiens 6-10 33792091-5 2021 The efflux function of P-gp required ATP, but AE reduced the intracellular ATP level. Adenosine Triphosphate 37-40 ATP binding cassette subfamily B member 1 Homo sapiens 23-27 33792091-5 2021 The efflux function of P-gp required ATP, but AE reduced the intracellular ATP level. Adenosine Triphosphate 75-78 ATP binding cassette subfamily B member 1 Homo sapiens 23-27 33547668-0 2021 Does the ATP-bound EQ mutant reflect the pre- or post-ATP hydrolysis state in the catalytic cycle of human P-glycoprotein (ABCB1)? Adenosine Triphosphate 9-12 ATP binding cassette subfamily B member 1 Homo sapiens 107-121 33547668-0 2021 Does the ATP-bound EQ mutant reflect the pre- or post-ATP hydrolysis state in the catalytic cycle of human P-glycoprotein (ABCB1)? Adenosine Triphosphate 9-12 ATP binding cassette subfamily B member 1 Homo sapiens 123-128 33547668-0 2021 Does the ATP-bound EQ mutant reflect the pre- or post-ATP hydrolysis state in the catalytic cycle of human P-glycoprotein (ABCB1)? Adenosine Triphosphate 54-57 ATP binding cassette subfamily B member 1 Homo sapiens 107-121 33547668-0 2021 Does the ATP-bound EQ mutant reflect the pre- or post-ATP hydrolysis state in the catalytic cycle of human P-glycoprotein (ABCB1)? Adenosine Triphosphate 54-57 ATP binding cassette subfamily B member 1 Homo sapiens 123-128 33547668-4 2021 The ATP hydrolysis-defective mutant of the catalytic glutamate residue of the Walker B motif (E556Q/E1201Q) has been used to determine the structure of the ATP-bound inward-closed conformation of P-gp. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 196-200 33547668-4 2021 The ATP hydrolysis-defective mutant of the catalytic glutamate residue of the Walker B motif (E556Q/E1201Q) has been used to determine the structure of the ATP-bound inward-closed conformation of P-gp. Adenosine Triphosphate 156-159 ATP binding cassette subfamily B member 1 Homo sapiens 196-200 33086089-2 2020 The overexpression of P-gp is responsible for ATP-dependent efflux of drugs that decrease their intracellular accumulation. Adenosine Triphosphate 46-49 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 33168729-6 2020 Similar to the efflux function of wild-type P-gp, we found that uptake by the 14A mutant is ATP hydrolysis-, substrate concentration-, and time-dependent. Adenosine Triphosphate 92-95 ATP binding cassette subfamily B member 1 Homo sapiens 44-48 33312947-2 2020 It is now well established that p-glycoprotein acts as an ATP dependent pump that pumps out small molecules from cells. Adenosine Triphosphate 58-61 ATP binding cassette subfamily B member 1 Homo sapiens 32-46 32816867-1 2020 As a member of anATP-dependent membrane transport proteins, P-Glycoprotein (P-gp) is known to pump substrates out of cells in the ATP-dependent mechanism. Adenosine Triphosphate 17-20 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 32945146-1 2020 Inhibiting the function of P-glycoprotein (P-gp) transporter, which causes drug efflux through adenosine triphosphate (ATP)-dependent manner, has become an effective strategy to conquer multidrug resistance (MDR) of cancer cells. Adenosine Triphosphate 119-122 ATP binding cassette subfamily B member 1 Homo sapiens 27-41 32945146-1 2020 Inhibiting the function of P-glycoprotein (P-gp) transporter, which causes drug efflux through adenosine triphosphate (ATP)-dependent manner, has become an effective strategy to conquer multidrug resistance (MDR) of cancer cells. Adenosine Triphosphate 119-122 ATP binding cassette subfamily B member 1 Homo sapiens 43-47 32816867-1 2020 As a member of anATP-dependent membrane transport proteins, P-Glycoprotein (P-gp) is known to pump substrates out of cells in the ATP-dependent mechanism. Adenosine Triphosphate 17-20 ATP binding cassette subfamily B member 1 Homo sapiens 76-80 32061787-2 2020 Besides other mechanisms, MDR is linked to the overexpression of ATP Binding Cassette (ABC), transporters, among which ABCB1 is the best characterized one. Adenosine Triphosphate 65-68 ATP binding cassette subfamily B member 1 Homo sapiens 119-124 32422185-6 2020 Further mechanism studies demonstrated that Que inhibited the ATP-driven transport activity of P-gp, which in turn increased the intracellular accumulation of Dox. Adenosine Triphosphate 62-65 ATP binding cassette subfamily B member 1 Homo sapiens 95-99 32044354-1 2020 The enhancement of drug efflux caused by ATP-binding cassette (ABC) transporters (including ABCG2 and ABCB1) overexpression is an important factor for multidrug resistance (MDR) in cancers. Adenosine Triphosphate 41-44 ATP binding cassette subfamily B member 1 Homo sapiens 102-107 32061772-6 2020 The function of P-gp required ATP, but CAN significantly reduced the intracellular ATP level. Adenosine Triphosphate 30-33 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 32111736-0 2020 In vivo FRET analyses reveal a role of ATP hydrolysis-associated conformational changes in human P-glycoprotein. Adenosine Triphosphate 39-42 ATP binding cassette subfamily B member 1 Homo sapiens 97-111 32331368-9 2020 Our work demonstrates that plasma membrane-associated Pgp prevents a complete ICD notwithstanding the release of ATP and HMGB1, and the exposure of CRT. Adenosine Triphosphate 113-116 ATP binding cassette subfamily B member 1 Homo sapiens 54-57 32111736-1 2020 P-glycoprotein (P-gp; also known as MDR1 or ABCB1) is an ATP-driven multidrug transporter that extrudes various hydrophobic toxic compounds to the extracellular space. Adenosine Triphosphate 57-60 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 32111736-1 2020 P-glycoprotein (P-gp; also known as MDR1 or ABCB1) is an ATP-driven multidrug transporter that extrudes various hydrophobic toxic compounds to the extracellular space. Adenosine Triphosphate 57-60 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 32111736-1 2020 P-glycoprotein (P-gp; also known as MDR1 or ABCB1) is an ATP-driven multidrug transporter that extrudes various hydrophobic toxic compounds to the extracellular space. Adenosine Triphosphate 57-60 ATP binding cassette subfamily B member 1 Homo sapiens 36-40 32111736-1 2020 P-glycoprotein (P-gp; also known as MDR1 or ABCB1) is an ATP-driven multidrug transporter that extrudes various hydrophobic toxic compounds to the extracellular space. Adenosine Triphosphate 57-60 ATP binding cassette subfamily B member 1 Homo sapiens 44-49 32111736-2 2020 P-gp consists of two transmembrane domains (TMDs) that form the substrate translocation pathway and two nucleotide-binding domains (NBDs) that bind and hydrolyze ATP. Adenosine Triphosphate 162-165 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 32111736-7 2020 In this study, we used a new FRET sensor that detects conformational changes in P-gp to investigate the role of ATP binding and hydrolysis during the conformational changes of human P-gp in living HEK293 cells. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 80-84 32111736-7 2020 In this study, we used a new FRET sensor that detects conformational changes in P-gp to investigate the role of ATP binding and hydrolysis during the conformational changes of human P-gp in living HEK293 cells. Adenosine Triphosphate 112-115 ATP binding cassette subfamily B member 1 Homo sapiens 182-186 32268494-3 2020 These are broad substrate-specificity ATP-dependent efflux pumps able to export toxins or drugs out of cells; for instance, ABCB1 (MDR1, or P-glycoprotein 1), overexpressed in most cancer cells, confers them multidrug resistance (MDR). Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 124-129 32268494-3 2020 These are broad substrate-specificity ATP-dependent efflux pumps able to export toxins or drugs out of cells; for instance, ABCB1 (MDR1, or P-glycoprotein 1), overexpressed in most cancer cells, confers them multidrug resistance (MDR). Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 131-135 32268494-3 2020 These are broad substrate-specificity ATP-dependent efflux pumps able to export toxins or drugs out of cells; for instance, ABCB1 (MDR1, or P-glycoprotein 1), overexpressed in most cancer cells, confers them multidrug resistance (MDR). Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 140-156 32232949-9 2020 Hence, the rotationally symmetric architecture of P-gp, which determines duality in ATP binding and rhodamine 123 interaction, also forms the basis for the existence of two independently operating outer gates. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 50-54 32260260-3 2020 At the expense of ATP hydrolysis, ABCB1 pumps a diverse range of substrates (including anticancer drugs) out of the cell, thereby reducing their intracellular concentration. Adenosine Triphosphate 18-21 ATP binding cassette subfamily B member 1 Homo sapiens 34-39 32166458-1 2020 Multidrug resistance (MDR) based on ATP-dependent efflux transporters (p-glycoprotein (p-gp)) remains a major obstacle in successful chemotherapy treatment. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 71-85 32166458-1 2020 Multidrug resistance (MDR) based on ATP-dependent efflux transporters (p-glycoprotein (p-gp)) remains a major obstacle in successful chemotherapy treatment. Adenosine Triphosphate 36-39 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 32092870-7 2020 The ATPase (adenosine triphosphatase) activity of the ABCB1 transporter in the presence or absence of WYE-354 was conducted in order to determine the impact of WYE-354 on ATP hydrolysis. Adenosine Triphosphate 4-7 ATP binding cassette subfamily B member 1 Homo sapiens 54-59 31982489-2 2020 MDR is caused by multiple mechanisms, involving ATP-binding cassette (ABC) transporters such as P-glycoprotein (P-gp), which reduces intracellular drug levels to sub-therapeutic concentrations. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 96-110 31982489-2 2020 MDR is caused by multiple mechanisms, involving ATP-binding cassette (ABC) transporters such as P-glycoprotein (P-gp), which reduces intracellular drug levels to sub-therapeutic concentrations. Adenosine Triphosphate 48-51 ATP binding cassette subfamily B member 1 Homo sapiens 112-116 31982489-7 2020 Celastrol inhibited both SERCA and P-gp to stimulate calcium-mediated autophagy and ATP depletion, thereby induced collateral sensitivity in MDR cancer cells. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 35-39 31917466-1 2020 P-glycoprotein (P-gp) is an ATP-dependent efflux pump that has a marked impact on the absorption, distribution, and excretion of therapeutic drugs. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 31917466-1 2020 P-glycoprotein (P-gp) is an ATP-dependent efflux pump that has a marked impact on the absorption, distribution, and excretion of therapeutic drugs. Adenosine Triphosphate 28-31 ATP binding cassette subfamily B member 1 Homo sapiens 16-20 32014648-2 2020 The overexpression of the ATP binding cassette (ABC) transporter, P-glycoprotein (P-gp), which significantly increases the efflux of certain anticancer drugs from tumor cells, produces MDR. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 66-80 32014648-2 2020 The overexpression of the ATP binding cassette (ABC) transporter, P-glycoprotein (P-gp), which significantly increases the efflux of certain anticancer drugs from tumor cells, produces MDR. Adenosine Triphosphate 26-29 ATP binding cassette subfamily B member 1 Homo sapiens 82-86 32054924-2 2020 ABCB1 consists of two transmembrane domains forming the substrate binding and translocation domain, and of two cytoplasmic nucleotide binding domains (NBDs) that provide the energy by binding and hydrolyzing ATP. Adenosine Triphosphate 208-211 ATP binding cassette subfamily B member 1 Homo sapiens 0-5 32148543-6 2020 In the presence of pulchinenosides, the ATP-dependent transport of N-methyl-quinidine mediated by P-glycoprotein was stimulated significantly. Adenosine Triphosphate 40-43 ATP binding cassette subfamily B member 1 Homo sapiens 98-112 31750987-3 2020 Growing evidence points to the involvement of P-glycoprotein (P-gp or ABCB1), an ATP-binding cassette transporter highly expressed on the luminal side of the blood brain barrier, in facilitating the clearance of Abeta from the brain. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 46-60 31750987-3 2020 Growing evidence points to the involvement of P-glycoprotein (P-gp or ABCB1), an ATP-binding cassette transporter highly expressed on the luminal side of the blood brain barrier, in facilitating the clearance of Abeta from the brain. Adenosine Triphosphate 81-84 ATP binding cassette subfamily B member 1 Homo sapiens 70-75 31991669-2 2020 The main thing responsible for MDR phenotypes are plasma-membranes associated with adenosine triphosphate (ATP) Binding Cassette (ABC) drug efflux transporters, such as the P-glycoprotein (Pgp) transporter that has the broadest spectrum of substrates. Adenosine Triphosphate 83-105 ATP binding cassette subfamily B member 1 Homo sapiens 173-187 31991669-2 2020 The main thing responsible for MDR phenotypes are plasma-membranes associated with adenosine triphosphate (ATP) Binding Cassette (ABC) drug efflux transporters, such as the P-glycoprotein (Pgp) transporter that has the broadest spectrum of substrates. Adenosine Triphosphate 83-105 ATP binding cassette subfamily B member 1 Homo sapiens 189-192 31991669-2 2020 The main thing responsible for MDR phenotypes are plasma-membranes associated with adenosine triphosphate (ATP) Binding Cassette (ABC) drug efflux transporters, such as the P-glycoprotein (Pgp) transporter that has the broadest spectrum of substrates. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 173-187 31963614-8 2020 Docking studies found that the binding site of caryophyllane sesquiterpene was next to the ATP binding domain of P-gp and that beta-caryophyllene possessed the stronger binding affinity and higher inhibition potential calculated by MM-PBSA. Adenosine Triphosphate 91-94 ATP binding cassette subfamily B member 1 Homo sapiens 113-117 31991669-2 2020 The main thing responsible for MDR phenotypes are plasma-membranes associated with adenosine triphosphate (ATP) Binding Cassette (ABC) drug efflux transporters, such as the P-glycoprotein (Pgp) transporter that has the broadest spectrum of substrates. Adenosine Triphosphate 107-110 ATP binding cassette subfamily B member 1 Homo sapiens 189-192 32064159-1 2020 The overexpression of one or multiple ATP-binding cassette (ABC) transporters such as ABCB1, ABCC1 or ABCG2 in cancer cells often leads to the development of multidrug resistance phenotype and consequent treatment failure. Adenosine Triphosphate 38-41 ATP binding cassette subfamily B member 1 Homo sapiens 86-91 31941029-1 2020 The development of multidrug resistance (MDR) in cancer patients driven by the overexpression of ATP-binding cassette (ABC) transporter ABCB1 or ABCG2 in cancer cells presents one of the most daunting therapeutic complications for clinical scientists to resolve. Adenosine Triphosphate 97-100 ATP binding cassette subfamily B member 1 Homo sapiens 136-141 31728254-2 2019 The expression of efflux transporters such as the ATP-binding cassette (ABC) transporter ABCB1 is an important resistance mechanism in therapy-refractory cancer cells. Adenosine Triphosphate 50-53 ATP binding cassette subfamily B member 1 Homo sapiens 89-94 31586724-4 2019 The nanoparticles loaded nitric oxide (NO) donor act as a NO nanoemitter to generate NO via a NIR light irradiation switch, which has a great capacity of reversing MDR via inhibiting the overexpression of P-glycoprotein (P-gp) and cell respiration with the reduction of both the adenosine triphosphate (ATP) content and mitochondrial membrane potential (DeltaPsim) in MDR tumor cells. Adenosine Triphosphate 279-301 ATP binding cassette subfamily B member 1 Homo sapiens 205-219 31586724-4 2019 The nanoparticles loaded nitric oxide (NO) donor act as a NO nanoemitter to generate NO via a NIR light irradiation switch, which has a great capacity of reversing MDR via inhibiting the overexpression of P-glycoprotein (P-gp) and cell respiration with the reduction of both the adenosine triphosphate (ATP) content and mitochondrial membrane potential (DeltaPsim) in MDR tumor cells. Adenosine Triphosphate 279-301 ATP binding cassette subfamily B member 1 Homo sapiens 221-225 31586724-4 2019 The nanoparticles loaded nitric oxide (NO) donor act as a NO nanoemitter to generate NO via a NIR light irradiation switch, which has a great capacity of reversing MDR via inhibiting the overexpression of P-glycoprotein (P-gp) and cell respiration with the reduction of both the adenosine triphosphate (ATP) content and mitochondrial membrane potential (DeltaPsim) in MDR tumor cells. Adenosine Triphosphate 303-306 ATP binding cassette subfamily B member 1 Homo sapiens 205-219 31586724-4 2019 The nanoparticles loaded nitric oxide (NO) donor act as a NO nanoemitter to generate NO via a NIR light irradiation switch, which has a great capacity of reversing MDR via inhibiting the overexpression of P-glycoprotein (P-gp) and cell respiration with the reduction of both the adenosine triphosphate (ATP) content and mitochondrial membrane potential (DeltaPsim) in MDR tumor cells. Adenosine Triphosphate 303-306 ATP binding cassette subfamily B member 1 Homo sapiens 221-225 31585733-3 2019 In ATPase assay, increased ATP consumption was observed as the concentrations of Tes, dihydroepiandrosterone (Dhea), androstenedione (Ado), and dihydrotestosterone (Dht), but not androstenediol (Adol), were increased, suggesting that these four androgens are transported by P-gp. Adenosine Triphosphate 3-6 ATP binding cassette subfamily B member 1 Homo sapiens 274-278 31774117-0 2019 ATP-dependent thermostabilization of human P-glycoprotein (ABCB1) is blocked by modulators. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 43-57 31774117-0 2019 ATP-dependent thermostabilization of human P-glycoprotein (ABCB1) is blocked by modulators. Adenosine Triphosphate 0-3 ATP binding cassette subfamily B member 1 Homo sapiens 59-64 31774117-3 2019 Here, we assessed how the binding of transport substrates or modulators in the TM region and the binding of ATP to the nucleotide-binding domains (NBDs) affect the thermostability of P-gp in a membrane environment. Adenosine Triphosphate 108-111 ATP binding cassette subfamily B member 1 Homo sapiens 183-187 31774117-5 2019 Our results show that P-gp is significantly thermostabilized (>22 C higher IT50) by the binding of ATP under non-hydrolyzing conditions (in the absence of Mg2+). Adenosine Triphosphate 99-102 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 31774117-6 2019 By using an ATP-binding-deficient mutant (Y401A) and a hydrolysis-deficient mutant (E556Q/E1201Q), we show that thermostabilization of P-gp requires binding of ATP to both NBDs and their dimerization. Adenosine Triphosphate 12-15 ATP binding cassette subfamily B member 1 Homo sapiens 135-139 31774117-6 2019 By using an ATP-binding-deficient mutant (Y401A) and a hydrolysis-deficient mutant (E556Q/E1201Q), we show that thermostabilization of P-gp requires binding of ATP to both NBDs and their dimerization. Adenosine Triphosphate 160-163 ATP binding cassette subfamily B member 1 Homo sapiens 135-139 31774117-7 2019 Additionally, we found that transport substrates do not affect the thermal stability of P-gp either in the absence or presence of ATP; in contrast, inhibitors of P-gp including tariquidar and zosuquidar prevent ATP-dependent thermostabilization in a concentration-dependent manner, by stabilizing the inward-open conformation. Adenosine Triphosphate 211-214 ATP binding cassette subfamily B member 1 Homo sapiens 162-166 31774117-8 2019 Altogether, our data suggest that modulators, which bind in the TM regions, inhibit ATP hydrolysis and drug transport by preventing the ATP-dependent dimerization of the NBDs of P-gp. Adenosine Triphosphate 84-87 ATP binding cassette subfamily B member 1 Homo sapiens 178-182 31774117-8 2019 Altogether, our data suggest that modulators, which bind in the TM regions, inhibit ATP hydrolysis and drug transport by preventing the ATP-dependent dimerization of the NBDs of P-gp. Adenosine Triphosphate 136-139 ATP binding cassette subfamily B member 1 Homo sapiens 178-182 31702922-4 2019 This hit compound represents a novel class of P-gp inhibitors that specifically targets and inhibits P-gp ATP hydrolysis, while not being transported by the pump. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 46-50 31702922-4 2019 This hit compound represents a novel class of P-gp inhibitors that specifically targets and inhibits P-gp ATP hydrolysis, while not being transported by the pump. Adenosine Triphosphate 106-109 ATP binding cassette subfamily B member 1 Homo sapiens 101-105 31810909-7 2019 RESULTS: Our results revealed an association between ATP-binding cassette (ABC) transporter gene ABCB1 and resistance to NAMPT inhibitor FK866 in both HCT116RFK866 cells and parental HCT116 cells. Adenosine Triphosphate 53-56 ATP binding cassette subfamily B member 1 Homo sapiens 97-102 31641149-1 2019 We used hydrogen-deuterium exchange mass spectrometry (HDX-MS) to obtain a comprehensive view of transporter dynamics (85.8% sequence coverage) occurring throughout the multidrug efflux transporter P-glycoprotein (P-gp) in three distinct conformational states: predominantly inward-facing apo P-gp, pre-hydrolytic (E552Q/E1197Q) P-gp bound to Mg+2-ATP, and outward-facing P-gp bound to Mg+2-ADP-VO4-3. Adenosine Triphosphate 343-351 ATP binding cassette subfamily B member 1 Homo sapiens 198-212 31641149-1 2019 We used hydrogen-deuterium exchange mass spectrometry (HDX-MS) to obtain a comprehensive view of transporter dynamics (85.8% sequence coverage) occurring throughout the multidrug efflux transporter P-glycoprotein (P-gp) in three distinct conformational states: predominantly inward-facing apo P-gp, pre-hydrolytic (E552Q/E1197Q) P-gp bound to Mg+2-ATP, and outward-facing P-gp bound to Mg+2-ADP-VO4-3. Adenosine Triphosphate 343-351 ATP binding cassette subfamily B member 1 Homo sapiens 214-218 31465208-2 2019 The excess mitochondrial reactive oxygen species (ROS) could not only inhibit the function of P-gp through insufficient adenosine triphosphate supply but also cause apoptosis in MDR cells. Adenosine Triphosphate 120-142 ATP binding cassette subfamily B member 1 Homo sapiens 94-98 30851394-6 2019 The purification strategies we present yield homogeneous functionally active wild type P-gp and its E556Q/E1201Q mutant, which is defective in carrying out ATP hydrolysis. Adenosine Triphosphate 156-159 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 31346154-1 2019 BACKGROUND The aim of this study was to investigate the influence of adenosine triphosphate (ATP)-binding cassette transporter subfamily B member 1 (ABCB1) gene polymorphism on the efficacy of Remifentanil. Adenosine Triphosphate 110-113 ATP binding cassette subfamily B member 1 Homo sapiens 166-171 31097669-2 2019 We used double electron electron resonance spectroscopy to uncover the basis of stimulation of P-glycoprotein adenosine 5"-triphosphate (ATP) hydrolysis by multiple substrates and illuminate how substrates and inhibitors differentially affect its transport function. Adenosine Triphosphate 110-135 ATP binding cassette subfamily B member 1 Homo sapiens 95-109 31097669-2 2019 We used double electron electron resonance spectroscopy to uncover the basis of stimulation of P-glycoprotein adenosine 5"-triphosphate (ATP) hydrolysis by multiple substrates and illuminate how substrates and inhibitors differentially affect its transport function. Adenosine Triphosphate 137-140 ATP binding cassette subfamily B member 1 Homo sapiens 95-109