PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9498792-0 1998 Post-translational processing of murine IL-1: evidence that ATP-induced release of IL-1 alpha and IL-1 beta occurs via a similar mechanism. Adenosine Triphosphate 60-63 interleukin 1 beta Mus musculus 98-107 9987822-4 1999 Compound 27c also dose dependently inhibited LPS-primed ATP-induced IL-1 beta release in mice. Adenosine Triphosphate 56-59 interleukin 1 beta Mus musculus 68-77 9498792-5 1998 Proteolysis of pro-IL-1 beta initiates within 7.5 min of ATP addition; 17-kDa mature IL-1 beta is observed first intracellularly and subsequently extracellularly. Adenosine Triphosphate 57-60 interleukin 1 beta Mus musculus 19-28 9498792-7 1998 Macrophages exposed to ATP continuously or only for a 15-min pulse release IL-1 alpha, IL-1 beta, and lactate dehydrogenase (LDH). Adenosine Triphosphate 23-26 interleukin 1 beta Mus musculus 87-96 9498792-9 1998 Ethacrynic acid and DIDS (4,4"-diisothiocyanato-stilbene-2,2"-disulfonic acid) block ATP-induced proteolysis of pro-IL-1 beta and prevent release of pro-IL-1 alpha/beta and LDH; they do not inhibit ATP-induced K+ (86Rb+) efflux. Adenosine Triphosphate 85-88 interleukin 1 beta Mus musculus 116-125 9498792-12 1998 These results indicate that ATP stimulates externalization of both IL-1 alpha and IL-1 beta. Adenosine Triphosphate 28-31 interleukin 1 beta Mus musculus 82-91 9498792-2 1998 Externalization and proteolytic processing of pro-IL-1 beta can be initiated by extracellular ATP. Adenosine Triphosphate 94-97 interleukin 1 beta Mus musculus 50-59 9096130-3 1997 We report here that treating striatal astrocytes in primary culture with IL-1beta for 22-24 hr enhances the ATP-evoked release of arachidonic acid (AA) with no effect on the ATP-induced accumulation of inositol phosphates. Adenosine Triphosphate 108-111 interleukin 1 beta Mus musculus 73-81 9096130-5 1997 Indeed, P2Y2 antisense oligonucleotides reduce the ATP-evoked release of AA only from IL-1beta-treated astrocytes. Adenosine Triphosphate 51-54 interleukin 1 beta Mus musculus 86-94 35545052-7 2022 Small interfering RNA (siRNA-based) silencing of each of these genes suppresses lipopolysaccharide- and ATP-induced inflammasome activation, as determined by interleukin-1beta release from primary cultured murine microglia. Adenosine Triphosphate 104-107 interleukin 1 beta Mus musculus 158-175 8683156-4 1996 In contrast, extracellular ATP promoted mature IL-1 beta release from both macrophage populations. Adenosine Triphosphate 27-30 interleukin 1 beta Mus musculus 47-56 8051418-5 1994 Previously, ATP was shown to promote the release and proteolytic maturation of IL-1 beta from LPS-stimulated murine peritoneal macrophages. Adenosine Triphosphate 12-15 interleukin 1 beta Mus musculus 79-88 8051418-6 1994 Tenidap, a new anti-inflammatory and antiarthritic agent, inhibited the release and maturation of IL-1 beta induced in vitro by ATP treatment of murine peritoneal macrophages. Adenosine Triphosphate 128-131 interleukin 1 beta Mus musculus 98-107 8195155-0 1994 Interleukin-1 beta maturation and release in response to ATP and nigericin. Adenosine Triphosphate 57-60 interleukin 1 beta Mus musculus 0-18 8195155-3 1994 The potassium-proton ionophore nigericin and ATP are known to promote the maturation and release of IL-1 beta from LPS-stimulated cells. Adenosine Triphosphate 45-48 interleukin 1 beta Mus musculus 100-109 8195155-9 1994 Post-translational maturation and release of IL-1 beta began immediately after ATP addition. Adenosine Triphosphate 79-82 interleukin 1 beta Mus musculus 45-54 8195155-11 1994 ATP, therefore, promoted both the proteolytic maturation of IL-1 beta and the release of the biologically active species in the absence of cell lysis. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 60-69 8195155-13 1994 ADP was less active than ATP at initiating the post-translational maturation and release of IL-1 beta and AMP, GTP, and UTP were totally inactive, ATP, nigericin, A204, and lasalocid promoted a rapid and complete loss of the potassium analog 86Rb+ from cells that were preloaded with this cation; valinomycin-treated cells released only a portion of the radiolabeled cation. Adenosine Triphosphate 25-28 interleukin 1 beta Mus musculus 92-101 8195155-16 1994 These data suggest that ATP and nigericin induce a net decrease in intracellular levels of K+ which is necessary for activation of the post-translational maturation of IL-1 beta. Adenosine Triphosphate 24-27 interleukin 1 beta Mus musculus 168-177 7876552-5 1995 Injection of ATP 2 h after LPS led to the rapid extracellular release of IL-1 beta, IL-1 alpha, lactate dehydrogenase, and beta-N-acetylglucosaminidase. Adenosine Triphosphate 13-16 interleukin 1 beta Mus musculus 73-82 33477467-6 2021 The results showed that under the action of adenosine-triphosphate (ATP), rTSST-1 significantly induced interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) production in mouse macrophages and the production was dose-dependent. Adenosine Triphosphate 68-71 interleukin 1 beta Mus musculus 104-121 35181718-9 2022 Moreover, bone marrow derived macrophages isolated from P2X4-deficient mice revealed a reduced ATP-mediated release of CCL-2, CC chemokine ligand 5 (CCL-5), Interleukin-1beta (IL-1beta) and IL-6. Adenosine Triphosphate 95-98 interleukin 1 beta Mus musculus 157-174 33929904-9 2021 Il1a and Il1b induction with LPS or LPS+ATP challenge was diminished. Adenosine Triphosphate 40-43 interleukin 1 beta Mus musculus 9-13 31500508-8 2020 In brief, the present study for the first time demonstrates that LIPUS inhibits the production of mature IL1B partially via SQSTM1-dependent autophagic degradation of PKM2 in LPS-ATP-treated macrophages, which may further ameliorate the synovial inflammation and gait patterns in animal models. Adenosine Triphosphate 179-182 interleukin 1 beta Mus musculus 105-109 30632647-8 2019 Similarly, GEN-27 dose-dependently weakened adenosine triphosphate-induced NLRP3 and IL-1beta in RAW264.7 cells. Adenosine Triphosphate 44-66 interleukin 1 beta Mus musculus 85-93 31664855-4 2020 We found that lack of HO-1 activated lipopolysaccharide (LPS) and ATP-treated bone marrow-derived macrophages, causing an increase in secreted levels of cleaved interleukin (IL)-1B, IL-18, and caspase-1, markers of increased inflammasome activity, whereas HO-1 overexpression suppressed IL-1B, NLRP3, and IL-18. Adenosine Triphosphate 66-69 interleukin 1 beta Mus musculus 287-292 31700106-13 2019 LPS + ATP increased NLRP3, caspase-1 and interleukin-1beta (IL-1beta). Adenosine Triphosphate 6-9 interleukin 1 beta Mus musculus 41-58 31700106-13 2019 LPS + ATP increased NLRP3, caspase-1 and interleukin-1beta (IL-1beta). Adenosine Triphosphate 6-9 interleukin 1 beta Mus musculus 60-68 31175486-4 2019 The results showed that ATP treatment induced lytic cell death morphologically resembling canonical pyroptosis in both MCC950-treated BMDMs and RAW264.7 cells, but did not cause the activation of caspase-1 (by detecting caspase-1p10 and mature interleukin-1beta) and cleavage of GSDMD. Adenosine Triphosphate 24-27 interleukin 1 beta Mus musculus 244-261 31336157-10 2019 Coptisine significantly attenuated mature interleukin (IL)-1beta secretion in RAW264.7 macrophages stimulated with LPS plus ATP, nigericin, or MSU, by blocking caspase-1 activation. Adenosine Triphosphate 124-127 interleukin 1 beta Mus musculus 42-64 31327964-9 2019 Our results show that melatonin prevents LPS and Adenosine triphosphate (ATP) induced NLRP3 inflammasome activation in murine microglia in vitro, evidenced by inhibition of NLRP3 expression, Apoptosis-associated speck-like protein containing a CARD (ASC) speck formation, caspase-1 cleavage and interleukin-1beta (IL-1beta) maturation and secretion. Adenosine Triphosphate 73-76 interleukin 1 beta Mus musculus 295-312 30761140-3 2019 Here we showed that paclitaxel pre-treatment greatly enhanced ATP- or nigericin-induced NLRP3 inflammasome activation as indicated by increased release of cleaved caspase-1 and mature IL-1beta, enhanced formation of ASC speck, and increased gasdermin D cleavage and pyroptosis. Adenosine Triphosphate 62-65 interleukin 1 beta Mus musculus 184-192 30776316-2 2019 ATP is essential for the release of nonclassically secreted cytokines such as IL-1beta but, paradoxically, has been reported to inhibit the release of classically secreted cytokines such as TNF. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 78-86 31089880-3 2019 To explain this extracellular ATP is a potent activator of the Nrlp3 inflammasome, which leads to the release of interleukin 1beta and interleukin 18, as well as several danger-associated molecular pattern molecules (DAMPs) that activate the mannan-binding lectin (MBL) pathway of the ComC, from cells of the innate immunity network. Adenosine Triphosphate 30-33 interleukin 1 beta Mus musculus 113-130 30896160-5 2019 The nitro-substituted quinazolin-4(3 H)-one 2k inhibited NLRP3 inflammasome (IC50 5 muM) via the suppression of IL-1beta release from ATP-stimulated J774A.1 cells. Adenosine Triphosphate 134-137 interleukin 1 beta Mus musculus 112-120 30628485-6 2019 When macrophages were stimulated with LPS followed by ATP to activate the inflammasome, we found a significant increase in secreted IL-1beta from WT cells compared with ASK1-deficient cells. Adenosine Triphosphate 54-57 interleukin 1 beta Mus musculus 132-140 30628485-8 2019 Subsequent ATP treatment stimulated an increase in cleaved caspase-1 and IL-1beta in WT BMDMs compared with ASK1-/- BMDMs. Adenosine Triphosphate 11-14 interleukin 1 beta Mus musculus 73-81 30628485-9 2019 Similarly, treatment of MH-S cells with LPS + ATP caused an increase in both cleaved caspase-1 and IL-1beta that was diminished by the ASK-1 inhibitor NQDI1. Adenosine Triphosphate 46-49 interleukin 1 beta Mus musculus 99-107 30099341-3 2018 LPS plus ATP initiated IL-1beta cleavage and release in mouse peritoneal macrophages and peaked at 4 h. Leucodin did not show significant toxicity within 200 muM and effectively inhibited pro-IL-1beta cleavage and release of mature-IL-1beta in macrophages. Adenosine Triphosphate 9-12 interleukin 1 beta Mus musculus 23-31 30455347-7 2019 RORgamma inverse agonists suppressed lipopolysaccharide (LPS)/ATP-stimulated IL-1beta secretion and expression of Il1b and Nlrp3 in BMDMs. Adenosine Triphosphate 62-65 interleukin 1 beta Mus musculus 77-85 30455347-7 2019 RORgamma inverse agonists suppressed lipopolysaccharide (LPS)/ATP-stimulated IL-1beta secretion and expression of Il1b and Nlrp3 in BMDMs. Adenosine Triphosphate 62-65 interleukin 1 beta Mus musculus 114-118 30248132-7 2018 Especially, a flavonoid baicalein significantly inhibited ATP-induced inflammation, including interleukin-1beta secretion, through inhibition of the ATP/P2X7R signaling. Adenosine Triphosphate 58-61 interleukin 1 beta Mus musculus 94-111 30541648-19 2018 CONCLUSIONS: Pannexin-1 may be involved in LPS and MV induced lung injury, which may be regulated by intracellular release of ATP to the extracellular site and binding to P2X7 receptor on the cell surface, thereby regulating active caspase-1 production and release, involving in the production of IL-1beta and other inflammatory factors eventually which leads to the occurrence and development of lung injury. Adenosine Triphosphate 126-129 interleukin 1 beta Mus musculus 297-305 28941191-6 2017 PNU282987 also significantly inhibited the activation of NLRP3 inflammasome and thus decreased the production of IL-1beta and IL-18 both in lipopolysaccharide (LPS)/ATP-stimulated BV2 microglia in vitro and spinal cord from EAE mice in vivo, while inverse effects were observed in alpha7nAChR knockout mice. Adenosine Triphosphate 165-168 interleukin 1 beta Mus musculus 113-121 28766178-1 2017 Asbestos and silica (exogenous danger) and adenosine triphosphate (ATP, endogenous danger-signaling molecule) synergistically increase IL-1beta release from endotoxin-primed macrophage, which is mediated by NOD-like receptor protein 3 (NLRP3) inflammasome. Adenosine Triphosphate 43-65 interleukin 1 beta Mus musculus 135-143 28766178-1 2017 Asbestos and silica (exogenous danger) and adenosine triphosphate (ATP, endogenous danger-signaling molecule) synergistically increase IL-1beta release from endotoxin-primed macrophage, which is mediated by NOD-like receptor protein 3 (NLRP3) inflammasome. Adenosine Triphosphate 67-70 interleukin 1 beta Mus musculus 135-143 28766178-4 2017 IL-1beta release was synergistically increased when the cells were exposed to both LPS and ATP or FTiO2, while IL-6 was readily released by LPS alone. Adenosine Triphosphate 91-94 interleukin 1 beta Mus musculus 0-8 28855315-10 2017 Concerning the second triggering factors, ROS production and ATP- and nigericin-induced IL1b secretion were suppressed by eplerenone in the LPS-primed BMDM. Adenosine Triphosphate 61-64 interleukin 1 beta Mus musculus 88-92 30002631-10 2018 Furthermore, the phosphorylation of AMPK induced by ATP in LPS-primed macrophages was significantly suppressed by cyclic stretch, indicating that cyclic stretch negatively regulates IL-1beta secretion through the inhibition of caspase-1 activity by attenuating the AMPK pathway. Adenosine Triphosphate 52-55 interleukin 1 beta Mus musculus 182-190 29375379-7 2017 The results showed that scutellarin dose-dependently reduced caspase-1 activation and decreased mature interleukin-1beta (IL-1beta) release in lipopolysaccharide (LPS)-primed macrophages upon ATP or nigericin stimulation, indicating that scutellarin inhibited NLRP3 inflammasome activation in macrophages. Adenosine Triphosphate 192-195 interleukin 1 beta Mus musculus 122-130 28942141-7 2017 CORM-3 suppressed caspase-1 activation and the secretion of interleukin (IL)-1beta and IL-18 in macrophages in response to lipopolysaccharide (LPS) and ATP. Adenosine Triphosphate 152-155 interleukin 1 beta Mus musculus 60-82 28605578-8 2017 We found that both LPS plus ATP and Abeta25-35 enhanced the conversion of pro-caspase-1 to cleaved-caspase-1 and increased the production of mature IL-1beta in BV-2 cells. Adenosine Triphosphate 28-31 interleukin 1 beta Mus musculus 148-156 28653829-2 2017 Our studies established that JC-171 dose dependently inhibited LPS/ATP-induced interleukin-1beta (IL-1beta) release from J774A.1 macrophages with an IC50 of 8.45 +- 1.56 muM. Adenosine Triphosphate 67-70 interleukin 1 beta Mus musculus 79-96 28653829-2 2017 Our studies established that JC-171 dose dependently inhibited LPS/ATP-induced interleukin-1beta (IL-1beta) release from J774A.1 macrophages with an IC50 of 8.45 +- 1.56 muM. Adenosine Triphosphate 67-70 interleukin 1 beta Mus musculus 98-106 28576828-7 2017 In LPS/ATP-stimulated bone marrow-derived macrophages (BMDMs), CLIC1 or CLIC4 siRNA transfection impaired transcription of IL-1beta, ASC speck formation, and secretion of mature IL-1beta. Adenosine Triphosphate 7-10 interleukin 1 beta Mus musculus 123-131 27730511-4 2017 P2X7 receptor activation by extracellular ATP leads to maturation and release of IL-1beta by immune cells, which stimulates the production of oxygen reactive species. Adenosine Triphosphate 42-45 interleukin 1 beta Mus musculus 81-89 29044426-1 2017 The P2X7 is an adenosine triphosphate (ATP)-gated ion channel involved in several facets of immune activation and neuronal function through its importance in interleukin (IL)-1beta secretion. Adenosine Triphosphate 15-37 interleukin 1 beta Mus musculus 158-180 29044426-1 2017 The P2X7 is an adenosine triphosphate (ATP)-gated ion channel involved in several facets of immune activation and neuronal function through its importance in interleukin (IL)-1beta secretion. Adenosine Triphosphate 39-42 interleukin 1 beta Mus musculus 158-180 28791016-9 2017 The inhibition effect of Dox on IL-1beta was also conspicuous in cells with lipopolysaccharide and ATP stimulation. Adenosine Triphosphate 99-102 interleukin 1 beta Mus musculus 32-40 28765650-6 2017 This decrease was associated with an inhibition of the activation of p38MAPK, JNK, NF-kappaB and NLRP3 inflammasome leading to a reduction of IL-1beta secretion in response to LPS and ATP in REDD1-/- BMDM. Adenosine Triphosphate 184-187 interleukin 1 beta Mus musculus 142-150 28576828-7 2017 In LPS/ATP-stimulated bone marrow-derived macrophages (BMDMs), CLIC1 or CLIC4 siRNA transfection impaired transcription of IL-1beta, ASC speck formation, and secretion of mature IL-1beta. Adenosine Triphosphate 7-10 interleukin 1 beta Mus musculus 178-186 28377486-1 2017 BACKGROUND: Extracellular adenosine triphosphate (ATP) binds as a danger signal to purinergic receptor P2X7 and promotes inflammasome assembly and interleukin-1beta expression. Adenosine Triphosphate 26-48 interleukin 1 beta Mus musculus 147-164 28725182-5 2017 In peripheral blood mononuclear cells from nAChR gene-deficient mice, we demonstrated that inhibition of ATP-dependent release of IL-1beta by acetylcholine (ACh), nicotine and PC depends on subunits alpha7, alpha9 and alpha10. Adenosine Triphosphate 105-108 interleukin 1 beta Mus musculus 130-138 28377486-1 2017 BACKGROUND: Extracellular adenosine triphosphate (ATP) binds as a danger signal to purinergic receptor P2X7 and promotes inflammasome assembly and interleukin-1beta expression. Adenosine Triphosphate 50-53 interleukin 1 beta Mus musculus 147-164 28377486-8 2017 However, priming with lipopolysaccharide, followed by stimulation with ATP, led to an activation of caspase 1 and interleukin-1beta in P2X7-competent macrophages. Adenosine Triphosphate 71-74 interleukin 1 beta Mus musculus 114-131 28298636-5 2017 We further demonstrate that P2X5 is required for ATP-mediated inflammasome activation and IL-1beta production by OCs, and that P2X5-deficient OC maturation is rescued in vitro by addition of exogenous IL-1beta. Adenosine Triphosphate 49-52 interleukin 1 beta Mus musculus 201-209 28096454-5 2017 CCCP, an uncoupler and antimycin A, an inhibitor of the mitochondrial electron transport chain, inhibited IL-1beta release induced by ATP but not by other stimuli. Adenosine Triphosphate 134-137 interleukin 1 beta Mus musculus 106-114 27601185-6 2017 The ATP-degradating enzyme attenuated the release of ATP and IL-1beta. Adenosine Triphosphate 4-7 interleukin 1 beta Mus musculus 61-69 28266627-6 2017 Furthermore, endogenous ATP and caspase-1 were required for mature IL-1beta production stimulated by CCN1 in keratinocytes. Adenosine Triphosphate 24-27 interleukin 1 beta Mus musculus 67-75 27601185-8 2017 These results strongly suggest that S. sanguinis has the activity to induce IL-1beta through the NLRP3 inflammasome in macrophage and DC and interaction of purinergic receptors with ATP released is involved in expression of the activity. Adenosine Triphosphate 182-185 interleukin 1 beta Mus musculus 76-84 28078039-10 2016 The expressions of caspase-1, NLRP3, IL-1beta, and IL-18 in LPS+ATP group were significantly higher than the control group by Western blot and ELISA. Adenosine Triphosphate 64-67 interleukin 1 beta Mus musculus 37-45 27474370-9 2017 CD40 ligation in Muller cells triggered phospholipase C-dependent ATP release that caused P2X7-dependent production of TNF-alpha and IL-1beta by macrophages. Adenosine Triphosphate 66-69 interleukin 1 beta Mus musculus 133-141 27980220-4 2017 Our results showed that berberine significantly increased ATP-induced inflammasome activation as reflected by enhanced pyroptosis as well as increased release of caspase-1p10 and mature interleukin-1beta (IL-1beta) in macrophages. Adenosine Triphosphate 58-61 interleukin 1 beta Mus musculus 205-213 27543123-4 2016 Using the inflammasome protocol, exposure of cardiomyocytes (CMs) to LPS followed by ATP or nigericin caused release of mature IL-1beta. Adenosine Triphosphate 85-88 interleukin 1 beta Mus musculus 127-135 28018360-5 2016 Our results showed that AMPK signaling was activated in murine macrophages upon ATP treatment, which was accompanied by inflammasome activation and pyroptosis as evidenced by rapid cell membrane rupture as well as mature interleukin (IL)-1beta and active caspase-1p10 release. Adenosine Triphosphate 80-83 interleukin 1 beta Mus musculus 221-243 27812336-0 2016 Piperine Suppresses Pyroptosis and Interleukin-1beta Release upon ATP Triggering and Bacterial Infection. Adenosine Triphosphate 66-69 interleukin 1 beta Mus musculus 35-52 27733681-9 2016 Importantly, PL and PLP reduced IL-1beta production induced by LPS and ATP, or by LPS alone, in mice. Adenosine Triphosphate 71-74 interleukin 1 beta Mus musculus 32-40 27812336-4 2016 The results showed that piperine dose-dependently inhibited ATP-induced pyroptosis, thereby suppressing interleukin-1beta (IL-1beta) or high mobility group box-1 protein (HMGB1) release in LPS-primed bone marrow-derived macrophages and J774A.1 cells. Adenosine Triphosphate 60-63 interleukin 1 beta Mus musculus 123-131 27385778-10 2016 These PM pores also facilitated the efflux of cytosolic ATP and influx of extracellular Ca(2+) Although lanthanides and Gsdmd deletion both suppressed PM pore activity and pyroptotic lysis, robust IL-1beta release was observed in lanthanide-treated BMDMs but not in Gsdmd-deficient cells. Adenosine Triphosphate 56-59 interleukin 1 beta Mus musculus 197-205 27528423-4 2016 The stimulation of lung TAMs with LPS+ATP increased the release of IL-1beta. Adenosine Triphosphate 38-41 interleukin 1 beta Mus musculus 67-75 27311643-6 2016 Treatment of mBMDCs with exogenous ATP induced release of high levels of IL-1beta and IL-18, and this release was also significantly inhibited by apyrase and A438079. Adenosine Triphosphate 35-38 interleukin 1 beta Mus musculus 73-81 27311643-10 2016 ATP induces ROS production via NADPH oxidase, and ROS activate inflammasomes, leading to caspase-1-dependent processing of pro-cytokines and release of IL-1beta and IL-18. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 152-160 27251615-3 2016 The receptor is activated by the release of ATP from intracellular sources that occurs during neurodegeneration, leading to microglial activation and inflammasome-mediated interleukin 1beta release that contributes to neuroinflammation. Adenosine Triphosphate 44-47 interleukin 1 beta Mus musculus 172-189 27533933-6 2016 We further showed that LPS plus ATP activated inflammasome, evidenced by the upregulation of caspase-1 and IL-1beta, which was suppressed by ZYVAD, a caspase-1 inhibitor. Adenosine Triphosphate 32-35 interleukin 1 beta Mus musculus 107-115 26456657-4 2015 Furthermore, disrupting the ATP-driven interaction by knocking-out P2X4R provoked an attenuation of P2X7-induced cell death, dye uptake and IL-1beta release in macrophages. Adenosine Triphosphate 28-31 interleukin 1 beta Mus musculus 140-148 26877061-2 2016 In the current study, we demonstrate that human and murine neutrophils express functional cell-surface P2X7R, which leads to ATP-induced loss of intracellular K(+), NLRP3 inflammasome activation and IL-1beta secretion. Adenosine Triphosphate 125-128 interleukin 1 beta Mus musculus 199-207 26481854-15 2016 There was an inverse correlation between levels of SHIP activity in PBMCs and induction of IL1beta production by lipopolysaccharide and adenosine triphosphate (R(2) = .88). Adenosine Triphosphate 136-158 interleukin 1 beta Mus musculus 91-98 26481854-18 2016 There is an inverse correlation between SHIP activity and induction of IL1beta production by lipopolysaccharide and adenosine triphosphate in PBMCs. Adenosine Triphosphate 116-138 interleukin 1 beta Mus musculus 71-78 26675345-5 2016 HCAG also reduced NLRP3 inflammasome-derived IL-1beta secretion by inhibiting the ATP-mediated phosphorylation of AKT and PKC-alpha/delta. Adenosine Triphosphate 82-85 interleukin 1 beta Mus musculus 45-53 27524862-5 2016 ATP-induced IL-1beta and lactate dehydrogenase (LDH) release were assessed by specific colorimetric assays. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 12-20 27524862-8 2016 ATP caused rapid IL-1beta release in a concentration-dependent manner, and this process was completely impaired by the P2X7 antagonist, AZ10606120. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 17-25 26584539-8 2015 In LPS-primed macrophages, Citral reduced ATP-induced IL-1beta secretion and caspase-1 activation, but did not affect LPS-induced NLRP3 protein expression. Adenosine Triphosphate 42-45 interleukin 1 beta Mus musculus 54-62 25626736-6 2015 In vitro, we demonstrated that the MUFA oleic acid can impede ATP-induced IL-1beta secretion from lipopolysaccharide- and SFA-primed cells in an AMPK-dependent manner. Adenosine Triphosphate 62-65 interleukin 1 beta Mus musculus 74-82 26100496-7 2015 Depletion of uric acid or ATP, or lack of ATP signaling attenuated ASH and prevented inflammasome activation and its major downstream cytokine, IL-1beta. Adenosine Triphosphate 42-45 interleukin 1 beta Mus musculus 144-152 26100496-10 2015 CONCLUSIONS: Our data indicate that the second signal in inflammasome activation and IL-1beta production in ASH results from the endogenous danger signals, uric acid and ATP. Adenosine Triphosphate 170-173 interleukin 1 beta Mus musculus 85-93 30338301-8 2015 IL-1beta secretion was analyzed by ELISA, mitochondrial function by JC-1 staining and ATP content. Adenosine Triphosphate 86-89 interleukin 1 beta Mus musculus 0-8 26056255-4 2015 We compared the ability of Tr1 cells versus Foxp3(+) Tregs to suppress IL-1beta production from macrophages following LPS and ATP stimulation. Adenosine Triphosphate 126-129 interleukin 1 beta Mus musculus 71-79 26091541-6 2015 Microglia were able to produce IL-1beta in response to different classical inflammasome activators, such as ATP, Nigericin or Alum. Adenosine Triphosphate 108-111 interleukin 1 beta Mus musculus 31-39 26320672-6 2015 Furthermore, in the LPS plus ATP cell model, we found that Fumigaclavine C dose-dependent inhibited IL-1beta release and caspase-1 activation. Adenosine Triphosphate 29-32 interleukin 1 beta Mus musculus 100-108 25944053-10 2015 miR-155 deficiency on DCs was also associated with impaired purinergic receptor signaling, as miR-155(-/-) DCs showed reduced chemotaxis and IL-1beta secretion upon stimulation with ATP, probably due to direct targeting of ectonucleoside triphosphate diphosphohydrolases (ENTPD) by miR-155. Adenosine Triphosphate 182-185 interleukin 1 beta Mus musculus 141-149 25867480-5 2015 Emodin treatment attenuated interleukin (IL)-1beta secretion via the inhibition of NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation induced by ATP, nigericin, and silica crystals. Adenosine Triphosphate 178-181 interleukin 1 beta Mus musculus 28-50 25580516-9 2015 Inhibitor experiments indicated that O157:H7-induced IL-1beta production was largely dependent upon caspase-1 activation and partially dependent upon ATP signalling and cathepsin B release, which were both involved in NLRP3 activation. Adenosine Triphosphate 150-153 interleukin 1 beta Mus musculus 53-61 25295542-8 2014 IL-1beta cleavage and secretion were impaired in HO-1-deficient macrophages, and CO-dependent processing of IL-1beta required the presence of bacteria-derived ATP. Adenosine Triphosphate 159-162 interleukin 1 beta Mus musculus 108-116 25681768-0 2015 Extracellular ATP induces P2X7 receptor activation in mouse Kupffer cells, leading to release of IL-1beta, HMGB1, and PGE2, decreased MHC class I expression and necrotic cell death. Adenosine Triphosphate 14-17 interleukin 1 beta Mus musculus 97-105 25681768-4 2015 Treatment of LPS-primed Kupffer cells with 3 mM ATP induced Ca(2+) influx, non-selective large pore formation, activation of MAPK, cell lysis, IL-1beta release, prostaglandin E2 (PGE2) release, high mobility group box1 (HMGB1) release, and major histocompatibility complex (MHC) class I shedding. Adenosine Triphosphate 48-51 interleukin 1 beta Mus musculus 143-151 25472006-8 2015 Treatment of DBA/2 susceptible mice with inflammasome inducers (i.e. nigericin and ATP) artificially increased pyroptosis and lowered the levels of NF-kappaB-related inflammatory cytokines, but restored IL-1beta to levels similar to those in C57BL/6 mice. Adenosine Triphosphate 83-86 interleukin 1 beta Mus musculus 203-211 25205101-3 2014 Here, we report that ATP-mediated suppression of IFNgamma production by Th17 cells can be overcome by genetic ablation of CD73 or by using IL1beta instead of TGFbeta to program Th17 cells ex vivo. Adenosine Triphosphate 21-24 interleukin 1 beta Mus musculus 139-146 25245808-5 2014 In addition, the secretion of interleukin-1beta (IL-1beta) and CCL-2 was enhanced significantly by ATP, in a time- and dose-dependent manner. Adenosine Triphosphate 99-102 interleukin 1 beta Mus musculus 30-47 25245808-5 2014 In addition, the secretion of interleukin-1beta (IL-1beta) and CCL-2 was enhanced significantly by ATP, in a time- and dose-dependent manner. Adenosine Triphosphate 99-102 interleukin 1 beta Mus musculus 49-57 25295542-10 2014 The ATP then bound to macrophage nucleotide P2 receptors, resulting in activation of the NALP3/IL-1beta inflammasome to amplify bacterial phagocytosis by macrophages. Adenosine Triphosphate 4-7 interleukin 1 beta Mus musculus 95-103 24789624-5 2014 We found that the exposure of RAW264.7 cells to LPS/ATP triggered the activation of caspase-1 (P<0.01) and the cleavage of interleukin (IL)-1beta (P<0.01), as well as the release of other cytokines, such as IL-18 (P<0.01) and IL-33 (P<0.01). Adenosine Triphosphate 52-55 interleukin 1 beta Mus musculus 126-148 25272939-0 2014 Losartan inhibits LPS + ATP-induced IL-1beta secretion from mouse primary macrophages by suppressing NALP3 inflammasome. Adenosine Triphosphate 24-27 interleukin 1 beta Mus musculus 36-44 25272939-4 2014 To further elucidate the molecular mechanism underlying the anti-IL-1beta property of losartan, we studied the LPS+ATP-induced activation of NALP3 inflammasome which controls the muturation and secretion of IL-1beta. Adenosine Triphosphate 115-118 interleukin 1 beta Mus musculus 207-215 25272939-5 2014 METHODS: LPS and ATP were used to stimulate the release of IL-1beta from thioglycollate-elicited macrophages from BALB/c mice. Adenosine Triphosphate 17-20 interleukin 1 beta Mus musculus 59-67 25272939-7 2014 RESULTS: In cultured thioglycollate-elicited macrophages, we observed that LPS + ATP greatly enhanced IL-1 beta secretion (6938.00 +/- 83.45; P < 0.05) and the mRNA levels of NALP3, caspase-1 which are two main components of NALP3 inflammasome (60.88 +/- 8.28; 1.31 +/- 0.04, P < 0.05 for both). Adenosine Triphosphate 81-84 interleukin 1 beta Mus musculus 102-111 25272939-11 2014 Losartan is able to suppress the LPS + ATP-induced production of IL-1beta protein. Adenosine Triphosphate 39-42 interleukin 1 beta Mus musculus 65-73 24685903-7 2014 Treatment of LPS-primed KUP5 cells with ATP also induced a high level of IL-1beta production, similar to that induced by SNP30. Adenosine Triphosphate 40-43 interleukin 1 beta Mus musculus 73-81 24789624-0 2014 Lipopolysaccharide/adenosine triphosphate induces IL-1beta and IL-18 secretion through the NLRP3 inflammasome in RAW264.7 murine macrophage cells. Adenosine Triphosphate 19-41 interleukin 1 beta Mus musculus 50-58 24603330-7 2014 Removing extracellular ATP with systemic apyrase treatment not only prevented IL-1beta accumulation but also the production of inflammasome-independent cytokines such as TNF and IL-10. Adenosine Triphosphate 23-26 interleukin 1 beta Mus musculus 78-86 24972036-4 2014 ATP can evoke the P2X7/caspase 1 dependent release of IL-1beta/IL-18 from EVs; these cytokines are associated with neutrophilia and are increased during exacerbations. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 54-62 24972036-5 2014 Thus we hypothesized that respiratory infections causes the release of EVs in the airway and that the raised ATP levels, present in respiratory disease, triggers the release of IL-1beta/IL-18, neutrophilia and subsequent disease exacerbations. Adenosine Triphosphate 109-112 interleukin 1 beta Mus musculus 177-185 24972036-9 2014 When exposed to ATP the EVs release IL-1beta/IL-18 via a P2X7/caspase-dependent mechanism. Adenosine Triphosphate 16-19 interleukin 1 beta Mus musculus 36-44 24475307-3 2014 We first demonstrated that extracellular ATP (2.5 mM) markedly increased the formation and activation of Nlrp3 inflammasomes in bone marrow macrophages (BMMs) from wild type (Asc+/+) mice resulting in activation of caspase-1 and IL-1beta production. Adenosine Triphosphate 41-44 interleukin 1 beta Mus musculus 229-237 24395802-6 2014 Neutrophils, but not bone marrow-derived macrophages, from cmo mice secreted increased IL-1beta in response to ATP, silica, and Pseudomonas aeruginosa compared with neutrophils from WT mice. Adenosine Triphosphate 111-114 interleukin 1 beta Mus musculus 87-95 23221343-7 2013 These data demonstrated that CaOx crystals trigger IL-1beta-dependent innate immunity via the NLRP3/ASC/caspase-1 axis in intrarenal mononuclear phagocytes and directly damage tubular cells, leading to the release of the NLRP3 agonist ATP. Adenosine Triphosphate 235-238 interleukin 1 beta Mus musculus 51-59 24454930-6 2014 The data indicated that the stimulation of J774 cells with LPS and ATP induces the features of pyroptosis, including the expression of IL-1beta mRNA and protein, activation of caspase-1, inflammasome formation and cell death. Adenosine Triphosphate 67-70 interleukin 1 beta Mus musculus 135-143 24454930-7 2014 Moreover, LL-37 inhibits the LPS/ATP-induced IL-1beta expression, caspase-1 activation, inflammasome formation, as well as cell death. Adenosine Triphosphate 33-36 interleukin 1 beta Mus musculus 45-53 23428419-10 2013 Chelation of intracellular Ca(2+) also caused suppression of ATP-induced IL-1beta and IL-18 release. Adenosine Triphosphate 61-64 interleukin 1 beta Mus musculus 73-81 23489421-11 2013 In vitro experiments confirmed that A438079 or apyrase treatment prevented the increase in IL-1beta that was evoked by macrophage and DC incubation with CrO and ATP. Adenosine Triphosphate 161-164 interleukin 1 beta Mus musculus 91-99 23454767-5 2013 Itgb3 deficiency in macrophages abrogated the inhibitory effect of MFGE8 on ATP-induced IL-1beta production. Adenosine Triphosphate 76-79 interleukin 1 beta Mus musculus 88-96 23580606-7 2013 In vitro studies in cells from adult mice showed that myocardial fibroblasts released IL-1beta and IL-18 when primed with lipopolysaccharide and subsequently exposed to the danger signal adenosine triphosphate, a molecule released after tissue damage during MI. Adenosine Triphosphate 187-209 interleukin 1 beta Mus musculus 86-94 23717478-5 2013 ATP induced the secretion of IL-1beta and chemokines by murine bone marrow-derived macrophages in vitro. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 29-37 23717478-6 2013 Furthermore, the intraperitoneal injection of ATP elevated the levels of IL-1beta and chemokines in the mouse peritoneal lavage. Adenosine Triphosphate 46-49 interleukin 1 beta Mus musculus 73-81 23428419-8 2013 Further, we found that ATP-induced IL-1beta and IL-18 release from LPS-primed BMDCs was suppressed by pretreatment with P2X7 antagonist A438079 or P2X4 antagonist TNP-ATP. Adenosine Triphosphate 23-26 interleukin 1 beta Mus musculus 35-43 23428419-8 2013 Further, we found that ATP-induced IL-1beta and IL-18 release from LPS-primed BMDCs was suppressed by pretreatment with P2X7 antagonist A438079 or P2X4 antagonist TNP-ATP. Adenosine Triphosphate 167-170 interleukin 1 beta Mus musculus 35-43 23454767-4 2013 MFGE8 inhibited necrotic cell-induced and ATP-dependent IL-1beta production by macrophages through mediation of integrin beta(3) and P2X7 receptor interactions in primed cells. Adenosine Triphosphate 42-45 interleukin 1 beta Mus musculus 56-64 23318584-4 2013 Depletion of iNOS resulted in increased accumulation of dysfunctional mitochondria in response to LPS and ATP, which was responsible for the increased IL-1beta production and caspase-1 activation. Adenosine Triphosphate 106-109 interleukin 1 beta Mus musculus 151-159 22895716-10 2012 Moreover, the IL-1beta expression in spinal microglia and the induction of tactile allodynia following the intrathecal administration of CGA depended on CatB, whereas those induced by the intrathecal administration of ATP or lysophosphatidic acid were CatB independent. Adenosine Triphosphate 218-221 interleukin 1 beta Mus musculus 14-22 23737642-4 2013 In microglia and the microglia cell line EOC20, ATP advanced the TNF-alpha/IFN-gamma-induced dye coupling, probably through the induction of IL-1beta release. Adenosine Triphosphate 48-51 interleukin 1 beta Mus musculus 141-149 21395581-3 2011 We recently reported ATP induces the release of an unconventional 20-kDa form of IL-1beta (p20-IL-1beta) from lipopolysaccharide-primed microglial cells. Adenosine Triphosphate 21-24 interleukin 1 beta Mus musculus 81-89 22147847-3 2012 Recent investigations revealed that airway exposure to allergen in sensitized individuals causes the release of ATP and uric acid, activating the NLRP3 inflammasome complex and cleaving pro-IL-1beta to mature IL-1beta through caspase-1. Adenosine Triphosphate 112-115 interleukin 1 beta Mus musculus 190-198 22147847-3 2012 Recent investigations revealed that airway exposure to allergen in sensitized individuals causes the release of ATP and uric acid, activating the NLRP3 inflammasome complex and cleaving pro-IL-1beta to mature IL-1beta through caspase-1. Adenosine Triphosphate 112-115 interleukin 1 beta Mus musculus 209-217 21306580-6 2011 Peritoneal mouse macrophages showed less ATP-induced permeabilization to ethidium bromide in the presence of colchicine, and less reactive oxygen species (ROS) formation, nitric oxide (NO) and interleukin (IL)-1beta release. Adenosine Triphosphate 41-44 interleukin 1 beta Mus musculus 193-215 21306580-8 2011 Finally, in vivo assays with mice inoculated with lipopolysaccharide and ATP showed diminished ROS, IL-1beta, interferon-gamma and NO production after colchicine treatment. Adenosine Triphosphate 73-76 interleukin 1 beta Mus musculus 100-108 22490780-3 2012 P2X7 receptors (P2X7Rs) expressed by inflammatory cells are stimulated by the danger signal extracellular ATP to activate the inflammasome and release IL-1beta. Adenosine Triphosphate 106-109 interleukin 1 beta Mus musculus 151-159 21709053-5 2012 The combination of ATP plus ivermectin also decreased the intracellular concentration of potassium and promoted the secretion of IL-1beta. Adenosine Triphosphate 19-22 interleukin 1 beta Mus musculus 129-137 22405772-5 2012 Treatment of mouse macrophage RAW264.7 cells with 1mM ATP induced high mobility group box 1 (HMGB1) release and IL-1beta production via activation of P2X7 receptor. Adenosine Triphosphate 54-57 interleukin 1 beta Mus musculus 112-120 22405772-6 2012 Knockdown of P2X4 receptor or removal of extracellular Ca(2+) suppressed ATP-induced release of both HMGB1 and IL-1beta. Adenosine Triphosphate 73-76 interleukin 1 beta Mus musculus 111-119 22136135-9 2011 Co-stimulation of microglia with LPS and adenosine triphosphate also resulted in increased phagocytosis, an effect inhibited by an interleukin-1 receptor antagonist, suggesting involvement of IL-1beta signaling. Adenosine Triphosphate 41-63 interleukin 1 beta Mus musculus 192-200 22002608-6 2011 Interestingly, Casp11(-/-) macrophages secreted IL-1beta normally in response to ATP and monosodium urate, indicating that caspase-11 is engaged by a non-canonical inflammasome. Adenosine Triphosphate 81-84 interleukin 1 beta Mus musculus 48-56 21395581-3 2011 We recently reported ATP induces the release of an unconventional 20-kDa form of IL-1beta (p20-IL-1beta) from lipopolysaccharide-primed microglial cells. Adenosine Triphosphate 21-24 interleukin 1 beta Mus musculus 95-103 21395581-5 2011 Here, we investigated the pathways involved in the ATP-induced production of p20-IL-1beta using lipopolysaccharide-primed mouse microglial cells. Adenosine Triphosphate 51-54 interleukin 1 beta Mus musculus 81-89 21395581-6 2011 The activation of P2X7 receptor by ATP triggered p20-IL-1beta production under acidic extracellular conditions. Adenosine Triphosphate 35-38 interleukin 1 beta Mus musculus 53-61 21395581-7 2011 ATP-induced p20-IL-1beta production was blocked by pepstatin A, a potent inhibitor of the lysosomal protease, cathepsin D. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 16-24 21395581-10 2011 Since facilitation of pore dilatation results in cytolysis accompanied with cytoplasmic pro-IL-1beta leakage, our data suggest the leaked pro-IL-1beta is processed into p20-IL-1beta by cathepsin D released after ATP stimulation under acidic extracellular conditions. Adenosine Triphosphate 212-215 interleukin 1 beta Mus musculus 142-150 21395581-10 2011 Since facilitation of pore dilatation results in cytolysis accompanied with cytoplasmic pro-IL-1beta leakage, our data suggest the leaked pro-IL-1beta is processed into p20-IL-1beta by cathepsin D released after ATP stimulation under acidic extracellular conditions. Adenosine Triphosphate 212-215 interleukin 1 beta Mus musculus 142-150 21151103-5 2011 Cytosolic mtDNA contributed to the secretion of IL-1beta and IL-18 in response to LPS and ATP. Adenosine Triphosphate 90-93 interleukin 1 beta Mus musculus 48-56 20639492-4 2010 In this study, we examined P2X(7)R function in resting human lung macrophages and mouse bone marrow-derived macrophages and found that ATP induced rapid release of the lysosomal cysteine proteases cathepsin B, K, L, and S and that was independent of the presence of the proinflammatory cytokines IL-1beta and IL-18. Adenosine Triphosphate 135-138 interleukin 1 beta Mus musculus 296-304 21048113-4 2010 After stimulation with ATP, an agonist of the NLRP3 inflammasome, knockdown of TRIM30 enhanced caspase-1 activation and increased production of IL-1beta in both J774 cells and bone marrow-derived macrophages. Adenosine Triphosphate 23-26 interleukin 1 beta Mus musculus 144-152 21437011-0 2010 Ivermectin-dependent release of IL-1beta in response to ATP by peritoneal macrophages from P2X(7)-KO mice. Adenosine Triphosphate 56-59 interleukin 1 beta Mus musculus 32-40 21437011-6 2010 One millimolar ATP increased the influx of extracellular calcium, decreased the intracellular concentration of potassium ([K(+)](i)) and stimulated the secretion of interleukin-1beta (IL-1beta) only in cells from WT mice. Adenosine Triphosphate 15-18 interleukin 1 beta Mus musculus 165-182 20522787-8 2010 Modulation of eATP levels with the ATP-degrading enzyme apyrase greatly reduced BLM-induced inflammatory cell recruitment, lung IL-1beta, and tissue inhibitor of metalloproteinase (TIMP)-1 production, while administration of ATP-gammaS, a stable ATP derivative, enhanced inflammation. Adenosine Triphosphate 15-18 interleukin 1 beta Mus musculus 128-136 20522787-8 2010 Modulation of eATP levels with the ATP-degrading enzyme apyrase greatly reduced BLM-induced inflammatory cell recruitment, lung IL-1beta, and tissue inhibitor of metalloproteinase (TIMP)-1 production, while administration of ATP-gammaS, a stable ATP derivative, enhanced inflammation. Adenosine Triphosphate 35-38 interleukin 1 beta Mus musculus 128-136 20522787-12 2010 CONCLUSIONS: ATP released from BLM-injured lung cells constitutes a major endogenous danger signal that engages the P2X(7) receptor/pannexin-1 axis, leading to IL-1beta maturation and lung fibrosis. Adenosine Triphosphate 13-16 interleukin 1 beta Mus musculus 160-168 21437011-6 2010 One millimolar ATP increased the influx of extracellular calcium, decreased the intracellular concentration of potassium ([K(+)](i)) and stimulated the secretion of interleukin-1beta (IL-1beta) only in cells from WT mice. Adenosine Triphosphate 15-18 interleukin 1 beta Mus musculus 184-192 21059855-6 2010 Whereas pretreatment of wild-type mice with P2X(7) antagonists, the ATP-degrading enzyme apyrase or IL-1 receptor antagonist, prevents CHS, IL-1beta injection restores CHS in P2X(7)-deficient mice. Adenosine Triphosphate 68-71 interleukin 1 beta Mus musculus 140-148 20071520-5 2010 Extensive phosphorylation of p38 MAPK in microglial cells in the dorsal horn was found to correlate with IL-1beta secretion following both LPS and ATP. Adenosine Triphosphate 147-150 interleukin 1 beta Mus musculus 105-113 20201036-5 2010 Entpd1(-/-) macrophages exposed to millimolar concentrations of ATP were more susceptible to cell death, released more IL-1beta and IL-18 after TLR2 or TLR4 priming, and incorporated the fluorescent dye Yo-Pro-1 more efficiently (suggestive of increased pore formation) than Entpd1(+/+) cells. Adenosine Triphosphate 64-67 interleukin 1 beta Mus musculus 119-127 19913495-2 2010 In cells from WT mice, 1 mM ATP increased the intracellular concentration of calcium ([Ca(2+)](i)), the uptake of ethidium bromide, the production of reactive oxygen species (ROS), the secretion of IL-1beta, the release of oleic acid and of lactate dehydrogenase; it decreased the intracellular concentration of potassium ([K(+)](i)). Adenosine Triphosphate 28-31 interleukin 1 beta Mus musculus 198-206 19767732-2 2009 Here we show that dying tumor cells release ATP, which then acts on P2X(7) purinergic receptors from DCs and triggers the NOD-like receptor family, pyrin domain containing-3 protein (NLRP3)-dependent caspase-1 activation complex ("inflammasome"), allowing for the secretion of interleukin-1beta (IL-1beta). Adenosine Triphosphate 44-47 interleukin 1 beta Mus musculus 277-294 19464323-2 2009 Activation of P2X7 receptors is the mechanism by which ATP stimulates the rapid maturation and release of IL-1beta from macrophages and microglial cells. Adenosine Triphosphate 55-58 interleukin 1 beta Mus musculus 106-114 19767732-2 2009 Here we show that dying tumor cells release ATP, which then acts on P2X(7) purinergic receptors from DCs and triggers the NOD-like receptor family, pyrin domain containing-3 protein (NLRP3)-dependent caspase-1 activation complex ("inflammasome"), allowing for the secretion of interleukin-1beta (IL-1beta). Adenosine Triphosphate 44-47 interleukin 1 beta Mus musculus 296-304 19547756-2 2009 We have previously shown that IL-1beta synthesis in the hippocampus is dependent on P2X(7) receptor (P2X(7)R), which is an ionotropic receptor of ATP. Adenosine Triphosphate 146-149 interleukin 1 beta Mus musculus 30-38 18552204-5 2008 Further, CNS glial and astrocyte-enriched cultures treated with a PAMP followed by an ATP pulse had significantly higher levels of supernatant IL-1beta and IL-33 than cultures receiving any single treatment (PAMP or ATP). Adenosine Triphosphate 86-89 interleukin 1 beta Mus musculus 143-151 18523246-0 2008 Lysophospholipids and ATP mutually suppress maturation and release of IL-1 beta in mouse microglial cells using a Rho-dependent pathway. Adenosine Triphosphate 22-25 interleukin 1 beta Mus musculus 70-79 18523246-10 2008 In addition, the microtubule inhibitor colchicine inhibited ATP-induced release of mIL-1beta similar to that of LPC and SPC. Adenosine Triphosphate 60-63 interleukin 1 beta Mus musculus 83-92 18523246-3 2008 In this study, we determined whether the lysophospholipids, i.e., LPC and sphingosylphosphorylcholine (SPC), modulate the ATP-induced release and processing of IL-1beta mediated by P2X7R in mouse MG6 microglial cells. Adenosine Triphosphate 122-125 interleukin 1 beta Mus musculus 160-168 18523246-11 2008 This suggests that the impairment of the microtubule reassembly may be associated with the inhibitory effects of LPC/SPC on ATP-induced mIL-1beta release. Adenosine Triphosphate 124-127 interleukin 1 beta Mus musculus 136-145 18523246-12 2008 Mutual suppression by ATP and LPC/SPC on the maturation of IL-1beta was observed in LPS-primed primary microglia. Adenosine Triphosphate 22-25 interleukin 1 beta Mus musculus 59-67 18523246-5 2008 However, these lysophospholipids inhibited ATP-induced caspase-1 activation that is usually followed by the release of mIL-1beta. Adenosine Triphosphate 43-46 interleukin 1 beta Mus musculus 119-128 18523246-6 2008 Conversely, ATP inhibited the release of pro-IL-1beta and mIL-1beta induced by LPC/SPC. Adenosine Triphosphate 12-15 interleukin 1 beta Mus musculus 45-53 18523246-6 2008 Conversely, ATP inhibited the release of pro-IL-1beta and mIL-1beta induced by LPC/SPC. Adenosine Triphosphate 12-15 interleukin 1 beta Mus musculus 58-67 18523246-7 2008 This suggests that lysophospholipids and ATP mutually suppressed each function to release IL-1beta. Adenosine Triphosphate 41-44 interleukin 1 beta Mus musculus 90-98 15240711-5 2004 IL-1beta, which also lacks a secretory signal peptide, may be released by ATP stimulation of the P2X(7)R. Adenosine Triphosphate 74-77 interleukin 1 beta Mus musculus 0-8 18490713-3 2008 Activation of purinergic P2X(7) receptors (P2X(7)R) by extracellular ATP is a key physiological inducer of rapid IL-1beta release from LPS-primed macrophage. Adenosine Triphosphate 69-72 interleukin 1 beta Mus musculus 113-121 18490713-5 2008 We used peritoneal macrophage from P2X(7)R(-/-) mice and found that release of IL-1alpha, IL-18, as well as IL-1beta, by ATP resulted exclusively from activation of P2X(7)R, release of all these IL-1 cytokines involved pannexin-1 (panx1), and that there was both a panx1-dependent and -independent component to IL-1beta release. Adenosine Triphosphate 121-124 interleukin 1 beta Mus musculus 108-116 18490713-5 2008 We used peritoneal macrophage from P2X(7)R(-/-) mice and found that release of IL-1alpha, IL-18, as well as IL-1beta, by ATP resulted exclusively from activation of P2X(7)R, release of all these IL-1 cytokines involved pannexin-1 (panx1), and that there was both a panx1-dependent and -independent component to IL-1beta release. Adenosine Triphosphate 121-124 interleukin 1 beta Mus musculus 311-319 18490713-10 2008 This provides the first direct demonstration of a distinct signaling mechanism responsible for ATP-induced release of pro-IL-1beta. Adenosine Triphosphate 95-98 interleukin 1 beta Mus musculus 122-130 17121814-2 2007 Blockade of pannexin-1 in macrophage endogenously expressing the ATP-gated P2X7 receptor (P2X7R) blocks the initial dye uptake, but not the ionic current, and also blocks processing and release of interleukin-1beta (IL-1beta) in response to P2X7R activation. Adenosine Triphosphate 65-68 interleukin 1 beta Mus musculus 197-214 17121814-2 2007 Blockade of pannexin-1 in macrophage endogenously expressing the ATP-gated P2X7 receptor (P2X7R) blocks the initial dye uptake, but not the ionic current, and also blocks processing and release of interleukin-1beta (IL-1beta) in response to P2X7R activation. Adenosine Triphosphate 65-68 interleukin 1 beta Mus musculus 216-224 17121814-10 2007 Thus, although pannexin-1 is required for IL-1beta release in response to maitotoxin, nigericin, and ATP, a mechanism distinct from pannexin-1 hemichannel activation must underlie the former two processes. Adenosine Triphosphate 101-104 interleukin 1 beta Mus musculus 42-50 16886979-5 2006 IL-1beta release was induced in mouse organotypic hippocampal slice cultures by proinflammatory stimuli [lipopolysaccharide (LPS) + adenosine triphosphate (ATP)] and measured with enzyme-linked immunosorbent assay (ELISA). Adenosine Triphosphate 132-154 interleukin 1 beta Mus musculus 0-8 16886979-5 2006 IL-1beta release was induced in mouse organotypic hippocampal slice cultures by proinflammatory stimuli [lipopolysaccharide (LPS) + adenosine triphosphate (ATP)] and measured with enzyme-linked immunosorbent assay (ELISA). Adenosine Triphosphate 156-159 interleukin 1 beta Mus musculus 0-8 16546100-4 2006 NALP3 was essential for the ATP-driven activation of caspase-1 in lipopolysaccharide-stimulated macrophages and for the efficient secretion of the caspase-1-dependent cytokines IL-1alpha, IL-1beta, and IL-18. Adenosine Triphosphate 28-31 interleukin 1 beta Mus musculus 188-196 15784009-8 2005 Disruption of mitochondrial membrane potential and ATP deprivation were observed in IL-1beta-treated ATDC5 cells, both of which were restored by L-NAME, AEBSF or uric acid. Adenosine Triphosphate 51-54 interleukin 1 beta Mus musculus 84-92 16301672-3 2005 In Bac1 murine macrophages, substitution of extracellular Cl- with gluconate produced a 10-fold increase in the rate and extent of ATP-induced IL-1beta processing and secretion, while reducing the EC50 for ATP by 5-fold. Adenosine Triphosphate 131-134 interleukin 1 beta Mus musculus 143-151 16301672-4 2005 Replacement of Cl- with gluconate also increased the potency of ATP as an inducer of mature IL-1beta secretion in primary mouse bone marrow-derived macrophages and in THP-1 human monocytes/macrophages. Adenosine Triphosphate 64-67 interleukin 1 beta Mus musculus 92-100 15075209-4 2004 In contrast, brief (5 min) treatment of intact macrophages with extracellular ATP (as an activator of the P2X(7) receptor) or nigericin before cell lysis markedly accelerated the in vitro processing of caspase-1 and IL-1beta. Adenosine Triphosphate 78-81 interleukin 1 beta Mus musculus 216-224 12759456-1 2003 In response to ATP binding, the P2X7R facilitates cation channel activation, nonspecific pore formation, rapid changes in plasma membrane morphology, and secretion of IL-1 beta from LPS-primed macrophages. Adenosine Triphosphate 15-18 interleukin 1 beta Mus musculus 167-176 15145076-0 2004 ATP-induced inhibition of gap junctional communication is enhanced by interleukin-1 beta treatment in cultured astrocytes. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 70-88 15145076-7 2004 Pretreatment with the pro-inflammatory cytokine interleukin-1beta (10 ng/ml, 24 h), which has no effect by itself on gap junctional communication, increases the inhibitory effect of ATP and astrocytes become sensitive to uridine 5"-triphosphate (UTP). Adenosine Triphosphate 182-185 interleukin 1 beta Mus musculus 48-65 15145076-8 2004 As indicated by the enhanced expression of P2Y(2) receptor mRNA, P2Y(2) receptors are responsible for the increased responses evoked by ATP and UTP in interleukin-1beta-treated cells. Adenosine Triphosphate 136-139 interleukin 1 beta Mus musculus 151-168 12796490-6 2003 ATP on its own had no effect on the synthesis of pro-IL-1beta, but a co-treatment with lipopolysaccharide and ATP led to the maturation and the release of IL-1beta by Schwann cells. Adenosine Triphosphate 110-113 interleukin 1 beta Mus musculus 155-163 12759456-6 2003 Blebbing was initiated at ATP concentrations > or = 3 mM, but optimal IL-1 beta release occurred at 1 mM ATP. Adenosine Triphosphate 108-111 interleukin 1 beta Mus musculus 73-82 12787061-5 2003 Treatment of lipopolysaccharide-primed microglia with adenosine triphosphate (ATP), an endogenous secondary stimulus, induced IL-1 beta and IL-18 release. Adenosine Triphosphate 54-76 interleukin 1 beta Mus musculus 126-135 12787061-5 2003 Treatment of lipopolysaccharide-primed microglia with adenosine triphosphate (ATP), an endogenous secondary stimulus, induced IL-1 beta and IL-18 release. Adenosine Triphosphate 78-81 interleukin 1 beta Mus musculus 126-135 12787061-7 2003 In addition, ATP induced release of mature IL-1 beta from IL-18-primed microglia. Adenosine Triphosphate 13-16 interleukin 1 beta Mus musculus 43-52 12759456-7 2003 P2X7R-dependent blebbing was abrogated in the presence of Rho-effector kinase inhibitors Fasudil and Y-27632, but ATP-induced IL-1 beta release was unaffected. Adenosine Triphosphate 114-117 interleukin 1 beta Mus musculus 126-135 12055263-5 2002 Blood stimulated with LPS or ATP individually generated minimal quantities of the leaderless polypeptide IL-1 beta, but sequential treatment of wild-type, but not P2X(7)R-deficient, blood with LPS and ATP yielded large amounts of cell-free cytokine. Adenosine Triphosphate 29-32 interleukin 1 beta Mus musculus 105-114 12044525-4 2002 RESULTS: Incubation of wild-type hepatocytes with a combination of cytokines (interleukin-1beta, tumor necrosis factor-alpha and interferon-gamma) and lipopolysaccharide (cytokines/LPS) inhibited insulin-stimulated glycogen synthesis and adenosine triphosphate (ATP) increase, and decreased the ketone body ratio (KBR) at 8-12 h, concomitant with expression of iNOS protein and NO production. Adenosine Triphosphate 238-260 interleukin 1 beta Mus musculus 78-124 12044525-4 2002 RESULTS: Incubation of wild-type hepatocytes with a combination of cytokines (interleukin-1beta, tumor necrosis factor-alpha and interferon-gamma) and lipopolysaccharide (cytokines/LPS) inhibited insulin-stimulated glycogen synthesis and adenosine triphosphate (ATP) increase, and decreased the ketone body ratio (KBR) at 8-12 h, concomitant with expression of iNOS protein and NO production. Adenosine Triphosphate 262-265 interleukin 1 beta Mus musculus 78-124 11943809-2 2002 Activation of P2X(7) purinoceptors by ATP or 3"-O-(4-benzoyl)-benzoyl ATP (BzATP) induces the formation of cytolytic pores and provokes release of interleukin-1beta from immune cells. Adenosine Triphosphate 38-41 interleukin 1 beta Mus musculus 147-164 11943809-9 2002 Finally, ADP and AMP stimulated the release of interleukin-1beta from ATP-primed mouse and human microglial cells. Adenosine Triphosphate 70-73 interleukin 1 beta Mus musculus 47-64 11706016-4 2002 The objective of the present study was to test the hypothesis that IL-1beta, IL-18, and/or caspase-1 activation contribute directly to macrophage cell death induced by LPS and ATP. Adenosine Triphosphate 176-179 interleukin 1 beta Mus musculus 67-75 11860279-1 2002 Activation of purinergic P2X7 receptors, principally by extracellular ATP, promotes the processing and release of the cytokine interleukin-1beta (IL-1beta) and induces cell death in activated microglia and macrophages. Adenosine Triphosphate 70-73 interleukin 1 beta Mus musculus 127-144 11016935-4 2001 In response to ATP, however, pro-IL-1 beta produced by the P2X(7)R(-/-) cells is not externalized or activated by caspase-1. Adenosine Triphosphate 15-18 interleukin 1 beta Mus musculus 33-42 10779799-0 2000 Kinetics and mechanism of ATP-dependent IL-1 beta release from microglial cells. Adenosine Triphosphate 26-29 interleukin 1 beta Mus musculus 40-49 10779799-3 2000 Addition of extracellular ATP to LPS-primed microglia caused a burst of release of a large amount of processed IL-1 beta. Adenosine Triphosphate 26-29 interleukin 1 beta Mus musculus 111-120 10779799-5 2000 In LPS-treated cells, ATP slightly increased the synthesis of pro-IL-1 beta. Adenosine Triphosphate 22-25 interleukin 1 beta Mus musculus 66-75 10779799-6 2000 Optimal ATP concentration for IL-1 beta secretion was between 3 and 5 mM, but significant release could be observed at concentrations as low as 1 mM. Adenosine Triphosphate 8-11 interleukin 1 beta Mus musculus 30-39 10779799-7 2000 At all ATP concentrations IL-1 beta release could be inhibited by increasing the extracellular K+ concentration. Adenosine Triphosphate 7-10 interleukin 1 beta Mus musculus 26-35 10779799-8 2000 ATP-dependent IL-1 beta release was also inhibited by 90 and 60% by the caspase inhibitors YVAD and DEVD, respectively. Adenosine Triphosphate 0-3 interleukin 1 beta Mus musculus 14-23