PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20151677-3 2010 The optimization of lipophilic substitution within a series of 4-benzyl-1-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperidin-4-amines provided ATP-competitive, nanomolar inhibitors with up to 150-fold selectivity for inhibition of PKB over the closely related kinase PKA. Adenosine Triphosphate 135-138 protein tyrosine kinase 2 beta Homo sapiens 223-226 21840393-0 2011 Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor. Adenosine Triphosphate 14-17 protein tyrosine kinase 2 beta Homo sapiens 62-66 21840393-3 2011 Here we tested whether ATP activates these kinases through Pyk2, a member of the focal adhesion kinase family. Adenosine Triphosphate 23-26 protein tyrosine kinase 2 beta Homo sapiens 59-63 21840393-4 2011 Pyk2 was rapidly and potently activated by treating corneal epithelial cells with ATP, and physical interaction of Pyk2 with the SFKs was enhanced. Adenosine Triphosphate 82-85 protein tyrosine kinase 2 beta Homo sapiens 0-4 21840393-5 2011 Disruption of Pyk2 signaling either by siRNA or by expression of a dominant-negative mutant led to inhibition of ATP-induced activation of the SFKs and the EGF receptor. Adenosine Triphosphate 113-116 protein tyrosine kinase 2 beta Homo sapiens 14-18 21840393-6 2011 Inhibiting Pyk2 activity also blocked ATP stimulation of healing of wounds in epithelial cell sheets. Adenosine Triphosphate 38-41 protein tyrosine kinase 2 beta Homo sapiens 11-15 21840393-7 2011 These data suggest that ATP stimulates sequential activation of Pyk2, SFKs, and the EGF receptor to induce cell migration. Adenosine Triphosphate 24-27 protein tyrosine kinase 2 beta Homo sapiens 64-68 21859844-3 2011 Extracellular ATP then acts on purinergic receptors, including P2Y2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization, which in turn stimulate fusion between Env-expressing membranes and membranes containing CD4 plus appropriate chemokine co-receptors. Adenosine Triphosphate 14-17 protein tyrosine kinase 2 beta Homo sapiens 81-111 21859844-3 2011 Extracellular ATP then acts on purinergic receptors, including P2Y2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization, which in turn stimulate fusion between Env-expressing membranes and membranes containing CD4 plus appropriate chemokine co-receptors. Adenosine Triphosphate 14-17 protein tyrosine kinase 2 beta Homo sapiens 113-117 19149480-2 2009 A number of small molecule inhibitors with well-defined, direct molecular interactions with PKB are now known, covering a range of mechanisms from ATP- or substrate-competitive inhibition, through allosteric modulation of the kinase activity, to inhibition of the phosphatidylinositol-dependent activation process. Adenosine Triphosphate 147-150 protein tyrosine kinase 2 beta Homo sapiens 92-95 12793995-0 2003 Differential role of PTK and ERK MAPK in superoxide impairment of K(ATP) and K(Ca) channel cerebrovasodilation. Adenosine Triphosphate 68-71 protein tyrosine kinase 2 beta Homo sapiens 21-24 18339875-5 2008 PF-562,271 is a potent, ATP-competitive, reversible inhibitor of FAK and Pyk2 catalytic activity with a IC(50) of 1.5 and 14 nmol/L, respectively. Adenosine Triphosphate 24-27 protein tyrosine kinase 2 beta Homo sapiens 73-77 18077599-1 2008 Previously, we showed that protein kinase B (Akt) activation increases intracellular ATP levels and decreases necrosis in renal proximal tubular cells (RPTC) injured by the nephrotoxicant S-(1, 2-dichlorovinyl)-l-cysteine (DCVC) (Shaik ZP, Fifer EK, Nowak G. Am J Physiol Renal Physiol 292: F292-F303, 2007). Adenosine Triphosphate 85-88 protein tyrosine kinase 2 beta Homo sapiens 27-43 15742376-3 2005 PTK/ZK functions as a competitive inhibitor at the ATP-binding site of the receptor kinase as shown here in kinetic experiments. Adenosine Triphosphate 51-54 protein tyrosine kinase 2 beta Homo sapiens 0-6 15634010-2 2005 The high homology between PKA and PKB includes very similar ATP binding sites and hence similar binding pockets for inhibitors, with only few amino acids that differ between the two kinases. Adenosine Triphosphate 60-63 protein tyrosine kinase 2 beta Homo sapiens 34-37 15634010-4 2005 Namely, the PKA to PKB exchanges F187L and Q84E enable the design of the selective inhibitors described herein which mimic ATP but extend further into a site not occupied by ATP. Adenosine Triphosphate 123-126 protein tyrosine kinase 2 beta Homo sapiens 19-22 15634010-4 2005 Namely, the PKA to PKB exchanges F187L and Q84E enable the design of the selective inhibitors described herein which mimic ATP but extend further into a site not occupied by ATP. Adenosine Triphosphate 174-177 protein tyrosine kinase 2 beta Homo sapiens 19-22 2736256-3 1989 ATP was the preferred phosphoryl donor for both PTKs (Km = 150 microM); however, in the case of lung PTK, GTP was able to partially replace ATP. Adenosine Triphosphate 0-3 protein tyrosine kinase 2 beta Homo sapiens 48-51 12822887-8 2003 The determination of the structure and position of the PH domain with respect to the known X-ray structure of the kinase active site could be useful in the rational design of potent and selective inhibitors of PDK1 and PKB by "linking" the free energies of binding of substrate (ATP) analogs with analogs of the inositol polar head group of the phospholipid second messenger. Adenosine Triphosphate 279-282 protein tyrosine kinase 2 beta Homo sapiens 219-222 8343210-4 1993 For testing we used an exogenous substrate kinase assay based on the phosphorylation of (Val5)-angiotensin II with radiolabelled ATP by the catalytic domain of the PTK encoded by the v-abl oncogene (p45 v-abl). Adenosine Triphosphate 129-132 protein tyrosine kinase 2 beta Homo sapiens 164-167 12569071-0 2003 Interleukin-1beta inhibits ATP-induced protein kinase B activation in renal mesangial cells by two different mechanisms: the involvement of nitric oxide and ceramide. Adenosine Triphosphate 27-30 protein tyrosine kinase 2 beta Homo sapiens 39-55 12569071-1 2003 1 Extracellular nucleotides, like ATP and UTP, have been shown to activate the protein kinase B (PKB) pathway in mesangial cells. Adenosine Triphosphate 34-37 protein tyrosine kinase 2 beta Homo sapiens 79-95 12569071-1 2003 1 Extracellular nucleotides, like ATP and UTP, have been shown to activate the protein kinase B (PKB) pathway in mesangial cells. Adenosine Triphosphate 34-37 protein tyrosine kinase 2 beta Homo sapiens 97-100 12569071-2 2003 In this study we report that the pro-inflammatory cytokine interleukin-1beta (IL-1beta) inhibits ATP-induced PKB activation. Adenosine Triphosphate 97-100 protein tyrosine kinase 2 beta Homo sapiens 109-112 12569071-3 2003 2 Pretreatment of mesangial cells with IL-1beta leads to a time-dependent decrease of ATP-induced PKB phosphorylation. Adenosine Triphosphate 86-89 protein tyrosine kinase 2 beta Homo sapiens 98-101 12569071-6 2003 A similar decrease in ATP-evoked PKB activation is obtained when cells were pretreated with the nitric oxide (NO) donor, (Z)-1-[2-Aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (Deta-NO), but not with the cell-permeable cGMP analogue, 8-bromo-cGMP. Adenosine Triphosphate 22-25 protein tyrosine kinase 2 beta Homo sapiens 33-36 12569071-8 2003 4 In addition, IL-1beta also triggers a short-term and transient inhibitory effect on ATP-induced PKB activation which is maximal after 2-5 min of pre-incubation with IL-1beta. Adenosine Triphosphate 86-89 protein tyrosine kinase 2 beta Homo sapiens 98-101 12569071-10 2003 Rather an involvement of the sphingolipid ceramide is likely, since IL-1beta triggers rapid ceramide formation and incubation of cells with the cell-permeable C6-ceramide blocked ATP-induced PKB phosphorylation. Adenosine Triphosphate 179-182 protein tyrosine kinase 2 beta Homo sapiens 191-194 12569071-11 2003 5 In summary, our data show that IL-1beta exerts both short-term and long-term inhibitory effects on ATP-stimulated PKB activation, the short-term effect probably involves ceramide formation, whereas the long-term effect is due to inducible NO synthase induction and subsequent NO formation. Adenosine Triphosphate 101-104 protein tyrosine kinase 2 beta Homo sapiens 116-119 8068042-2 1994 Drugs which bind to the ATP site of PTK, such as genistein, are common inhibitors to both types of enzymes. Adenosine Triphosphate 24-27 protein tyrosine kinase 2 beta Homo sapiens 36-39 25712051-8 2015 Fenretinide also shows intermediate binding at FAK"s FERM domain and interacts at the ATP-binding site of the closest FAK analogue, PYK2. Adenosine Triphosphate 86-89 protein tyrosine kinase 2 beta Homo sapiens 132-136 31746084-6 2020 Furthermore, stimulation of MSC migration induced by Yoda1 as well as ATP was suppressed by PF431396, a PYK2 kinase inhibitor, or U0126, an inhibitor of the mitogen-activated protein kinase MEK/ERK signaling pathway. Adenosine Triphosphate 70-73 protein tyrosine kinase 2 beta Homo sapiens 104-108 31746084-7 2020 Collectively, these results suggest that activation of the Piezo1 channel stimulates MSC migration via inducing ATP release and subsequent activation of the P2 receptor purinergic signalling and downstream PYK2 and MEK/ERK signaling pathways, thus revealing novel insights into the molecular and signalling mechanisms regulating MSC migration. Adenosine Triphosphate 112-115 protein tyrosine kinase 2 beta Homo sapiens 206-210 30637731-6 2019 Part of phosphorylated Pyk2 translocated from cytosol to mitochondria which has been previously shown to augment mitochondrial Ca2+ uptake and enhance adenosine triphosphate generation. Adenosine Triphosphate 151-173 protein tyrosine kinase 2 beta Homo sapiens 23-27 25853099-1 2015 Heat shock protein 90 (Hsp90) is an adenosine triphosphate dependent molecular chaperone in eukaryotic cells that regulates the activation and maintenance of numerous regulatory and signaling proteins including epidermal growth factor receptor, human epidermal growth factor receptor 2, mesenchymal-epithelial transition factor, cyclin-dependent kinase-4, protein kinase B, hypoxia-inducible factor 1alpha, and matrix metalloproteinase-2. Adenosine Triphosphate 36-58 protein tyrosine kinase 2 beta Homo sapiens 356-405 22224416-7 2012 ATP increased phosphorylated PKB (Akt) and ERK1/2 levels; an effect antagonized by suramin, reactive blue-2, the PI3-kinase inhibitor, wortmannin, PKB inhibitor, API-2, and MAPK inhibitor, PD98059. Adenosine Triphosphate 0-3 protein tyrosine kinase 2 beta Homo sapiens 29-32 22224416-7 2012 ATP increased phosphorylated PKB (Akt) and ERK1/2 levels; an effect antagonized by suramin, reactive blue-2, the PI3-kinase inhibitor, wortmannin, PKB inhibitor, API-2, and MAPK inhibitor, PD98059. Adenosine Triphosphate 0-3 protein tyrosine kinase 2 beta Homo sapiens 147-150