PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 35510853-3 2022 Here, we developed a leak-free inducible CRISPRi/a system by integrating the DiCre/loxP regulon to allow the expression of dCas9-GCN5/-Sir2a upon transient induction with rapamycin, which allows convenient transcriptional regulation of a gene of interest by introducing a guide RNA targeting its transcription start region. Sirolimus 171-180 lysine acetyltransferase 2A Homo sapiens 129-133