PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15285037-3 2004 Insulin-MTX conjugate was purified by Sephadex G-25 column and analyzed by high performance liquid chromatography. sephadex 38-46 insulin Homo sapiens 0-7 11517012-8 2001 Sephadex G-100 chromatography of the sera showed most of insulin immunoreactivity present in the void volume which was retained by an affinity column with anti-human-immunoglobulin G antibodies (87% and 95% from patients 1 and 2 respectively). sephadex 0-14 insulin Homo sapiens 57-64 12579844-7 2002 The quantity of insulin in the insulin-liposome mixture sample after separation by ultracentrifuge or by Sephadex G-75 determined by HPLC, the combination percent was only 0.2%, indicating low interaction between insulin and liposome. sephadex 105-118 insulin Homo sapiens 16-23 12579844-7 2002 The quantity of insulin in the insulin-liposome mixture sample after separation by ultracentrifuge or by Sephadex G-75 determined by HPLC, the combination percent was only 0.2%, indicating low interaction between insulin and liposome. sephadex 105-118 insulin Homo sapiens 31-38 12579844-7 2002 The quantity of insulin in the insulin-liposome mixture sample after separation by ultracentrifuge or by Sephadex G-75 determined by HPLC, the combination percent was only 0.2%, indicating low interaction between insulin and liposome. sephadex 105-118 insulin Homo sapiens 31-38 11257802-3 2001 The expressed RGD-proinsulin was refolded and purified by Sephadex G50 and DEAE-Sephadex A25 separations. sephadex 58-66 insulin Homo sapiens 18-28 11257802-3 2001 The expressed RGD-proinsulin was refolded and purified by Sephadex G50 and DEAE-Sephadex A25 separations. sephadex 80-88 insulin Homo sapiens 18-28 2146119-2 1990 Incubation of endosomal fractions containing 125I-insulin in isotonic KCl at 30 degrees C resulted in a rapid loss of insulin integrity as judged from trichloroacetic acid precipitability, Sephadex G-50 chromatography, immunoreactivity and receptor binding ability, with a maximum at pH 5-6 (t1/2: 10, 10, 6 and 6 min, respectively). sephadex 189-197 insulin Homo sapiens 50-57 7486987-4 1995 After simple downstream processing and only one step of Sephadex G50 purification, 150 mg recombinant Lys-human proinsulin with a purity of up to 90% could be obtained easily from 1 L of high density fermentation medium. sephadex 56-64 insulin Homo sapiens 112-122 8721774-6 1996 Release of intracellular insulin and its degradation in vitro was determined by incubating cells with 600 pmol/l of 125I-insulin for 60 min at 37 degrees C, acid washing cells, and re-incubating in insulin-free buffer at 37 degrees C. The radioactivity released by cells was characterized by trichloroacetic acid precipitability, Sephadex G-50 column chromatography, and re-binding to fresh cells. sephadex 330-338 insulin Homo sapiens 25-32 1414126-4 1992 The isolation of human proinsulin-S-sulfonate was accomplished by ion exchange chromatography on QAE-sephadex A-25, followed by gel filtration on sephadex G-50. sephadex 146-159 insulin Homo sapiens 23-33 1414126-6 1992 The folding mixture was then chromatographed on sephadex G-50 and purified proinsulin was obtained. sephadex 48-61 insulin Homo sapiens 75-85 3933801-3 1985 In addition to their behavior on Sephadex G-50, the immunoactive insulin-related materials from the microbial sources behaved like authentic vertebrate insulins in their ability to be adsorbed to and eluted from disposable octadecasilylsilica cartridges, DEAE-Sephadex, DEAE-cellulose, and one system of high-pressure liquid chromatography (HPLC). sephadex 33-46 insulin Homo sapiens 65-72 3276679-4 1988 Insulin-sized products were obtained by Sephadex G-50 filtration. sephadex 40-48 insulin Homo sapiens 0-7 2829843-2 1987 Reconstitution of the receptor kinase into leaky vesicles containing phosphatidylcholine and phosphatidylethanolamine (1:1, w/w) by detergent removal on Sephadex G-50 results in the complete loss of receptor kinase sensitivity to activation by insulin. sephadex 153-166 insulin Homo sapiens 244-251 3294822-4 1987 Removal of the dithiothreitol and pH neutralization (pH 7.60) by rapid Sephadex G-50 gel filtration resulted in the preservation of tracer insulin binding activity. sephadex 71-79 insulin Homo sapiens 139-146 3542649-3 1987 With this semiquantitative procedure, the monomer form of insulin was separated from aggregates during centrifuge desalting on Sephadex G-25. sephadex 127-140 insulin Homo sapiens 58-65 2951732-6 1987 The difference in MAP-2 kinase activity in extract supernatants from control and insulin-treated cells is also preserved after rapid chromatography on Sephadex G-25. sephadex 151-164 insulin Homo sapiens 81-88 3537496-6 1986 Following Sephadex G-50 gel filtration, the patient"s insulin eluted after the void volume. sephadex 10-23 insulin Homo sapiens 54-61 3077602-2 1986 Fractionation on Sephadex G50 gel filtration reveals that the apparent immunoreactivity corresponds for the most part to 6000 dalton insulin. sephadex 17-25 insulin Homo sapiens 133-140 3511852-6 1986 This procedure was based on the observation that the monomer species of insulin was separated from insulin aggregates due to its interaction with the devoided gel matrix of Sephadex G-25 and remained on the column following centrifugation. sephadex 173-186 insulin Homo sapiens 72-79 3511852-6 1986 This procedure was based on the observation that the monomer species of insulin was separated from insulin aggregates due to its interaction with the devoided gel matrix of Sephadex G-25 and remained on the column following centrifugation. sephadex 173-186 insulin Homo sapiens 99-106 3516229-3 1986 The insulin-like substance eluted from a Sephadex G-50 column in the same manner as native insulin, and its concentration exceeded the plasma level. sephadex 41-49 insulin Homo sapiens 4-11 3910407-4 1985 In addition, no damage of insulin molecules was found after the use of the latter method and the purification on QAE Sephadex A-25 yielded a homogenous preparation of insulin. sephadex 117-125 insulin Homo sapiens 167-174 4084539-1 1985 Purified human placental insulin receptors were incorporated into small unilamellar phospholipid vesicles by the addition of n-octyl beta-glucopyranoside solubilized phospholipids, followed by removal of the detergent on a Sephadex G-50 gel filtration column and extensive dialysis. sephadex 223-236 insulin Homo sapiens 25-32 3541897-8 1986 Scatchard plots based on insulin-binding data from Sephadex chromatography or TCA precipitation were identical. sephadex 51-59 insulin Homo sapiens 25-32 3510222-5 1986 Sephadex G-50 column chromatography of the intracellular insulin demonstrated that more than 95% of this pool coeluted with native insulin. sephadex 0-8 insulin Homo sapiens 57-64 3510222-5 1986 Sephadex G-50 column chromatography of the intracellular insulin demonstrated that more than 95% of this pool coeluted with native insulin. sephadex 0-8 insulin Homo sapiens 131-138 3510002-3 1986 The free insulin peak eluted from a Sephadex G-50 column was subsequently chromatographed on a Bio-Gel P-30 column. sephadex 36-44 insulin Homo sapiens 9-16 3889041-7 1985 Insulin extracted from the patient"s serum was identical to normal insulin by the criteria of Sephadex chromatography, placental membrane insulin receptor binding, and stimulation of 2-deoxyglucose uptake in isolated rat adipocytes. sephadex 94-102 insulin Homo sapiens 0-7 6368202-8 1984 When the conditioned medium was subjected to gel filtration on a Sephadex G-100 column, the major part of insulin-like activity eluted in the position corresponding to an apparent molecular weight between RNAase and insulin markers. sephadex 65-79 insulin Homo sapiens 106-113 6368202-8 1984 When the conditioned medium was subjected to gel filtration on a Sephadex G-100 column, the major part of insulin-like activity eluted in the position corresponding to an apparent molecular weight between RNAase and insulin markers. sephadex 65-79 insulin Homo sapiens 216-223 6322744-2 1983 Examination by chromatography on Sephadex G-75 of the degradation products formed from insulin showed A chain, and B chain rich-A chain aggregate as previously found with rat pancreatic islets. sephadex 33-46 insulin Homo sapiens 87-94 6341131-2 1983 The high resolving power of HPLC enabled us to separate what has been considered "intact insulin" by Sephadex G-50 chromatography or TCA precipitability into two additional peaks that had decreased biochemical properties with respect to immunoprecipitability and receptor binding but not decreased TCA precipitability. sephadex 101-109 insulin Homo sapiens 89-96 7013817-7 1981 Sephadex G-75 gel filtration of iodocompounds derived from cells incubated at 37 degrees C with 125I-labeled insulin for 5, 20 min and 1 h showed a progressive decrease in intact insulin, and an increase in the peak representing insulin breakdown products. sephadex 0-8 insulin Homo sapiens 109-116 7052070-5 1982 Sephadex G-75 chromatography of the acid-soluble products of insulin digestion by cathepsin D or L suggested that thiols can reduce disulphide bonds in proteins after limited proteolysis. sephadex 0-8 insulin Homo sapiens 61-68 7013817-7 1981 Sephadex G-75 gel filtration of iodocompounds derived from cells incubated at 37 degrees C with 125I-labeled insulin for 5, 20 min and 1 h showed a progressive decrease in intact insulin, and an increase in the peak representing insulin breakdown products. sephadex 0-8 insulin Homo sapiens 179-186 7013817-7 1981 Sephadex G-75 gel filtration of iodocompounds derived from cells incubated at 37 degrees C with 125I-labeled insulin for 5, 20 min and 1 h showed a progressive decrease in intact insulin, and an increase in the peak representing insulin breakdown products. sephadex 0-8 insulin Homo sapiens 179-186 6987656-2 1980 Rat liver insulin was shown to be indistinguishable from genuine insulin by radioimmunoassay, Sephadex chromatography, bioassay, and antibody neutralization. sephadex 94-102 insulin Homo sapiens 10-17 7014299-11 1981 By this time 96% of the radioactivity migrated with insulin when subjected to gel filtration on Sephadex G-50, 4% was in the void volume, and nothing in the total column volume or later. sephadex 96-109 insulin Homo sapiens 52-59 7002687-5 1980 The major degradation product after 120 min coeluted with insulin from Sephadex G50 and was precipitated by anti-insulin antibody but to a lesser degree than intact insulin. sephadex 71-79 insulin Homo sapiens 58-65 6254825-3 1980 Insulin treatment significantly altered the elution profile of deproteinized muscle extracts chromatographed on Sephadex G-25 columns, particularly in fraction II, which contains the insulin mediator. sephadex 112-120 insulin Homo sapiens 0-7 109340-8 1979 The activity was eluted from Sephadex G200 as a single peak and had properties consistent with those of the insulin-specific protease (ISP). sephadex 29-42 insulin Homo sapiens 108-115 4501116-0 1972 Isolation of the insulin receptor of liver and fat-cell membranes (detergent-solubilized-( 125 I)insulin-polyethylene glycol precipitation-sephadex). sephadex 139-147 insulin Homo sapiens 17-24 4323127-1 1971 When plasma is filtered on Sephadex G-50, insulin immunoreactivity is recovered in two peaks. sephadex 27-40 insulin Homo sapiens 42-49 19999729-2 1973 Reaction for 30 min in dimethylformamide afforded several p-chloromercuribenzoyl-insulin derivatives which were characterized by DEAE-Sephadex and Sephadex G-50 chromatography, amino acid analysis, ultraviolet spectroscopy, deamination and trypsin digestion. sephadex 134-142 insulin Homo sapiens 81-88 4673672-0 1972 Heterogeneity of the Fourth International Standard for insulin by gel-chromatography on sephadex. sephadex 88-96 insulin Homo sapiens 55-62 5725280-12 1968 Most of the insulin-like activity retained on Dowex-50 ("bound insulin") is eluted off Sephadex G-75 (acetic acid-NaCl) at the same column volume as the small molecular weight nonsuppressible ILA. sephadex 87-100 insulin Homo sapiens 63-71