PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 35227018-3 2022 While pendrin drives chloride reabsorption and bicarbonate, thiocyanate or iodide secretion within the apical compartment, CFTR represents a pathway for the apical efflux of chloride, bicarbonate, and possibly iodide. Iodides 210-216 CF transmembrane conductance regulator Homo sapiens 123-127 35227018-9 2022 In the thyroid, CFTR and pendrin might have overlapping functions in driving the apical flux of iodide within the follicular lumen. Iodides 96-102 CF transmembrane conductance regulator Homo sapiens 16-20 29779372-6 2018 The method was implemented using a 10 x 10 micropillar array (size = 3 x 3 mm) on CFTR-expressing epithelial cells, in which CFTR chloride channel function was measured from fluorescence in response to iodide addition using a genetically encoded cytoplasmic yellow fluorescent protein halide indicator. Iodides 202-208 CF transmembrane conductance regulator Homo sapiens 125-129 30641980-9 2019 In the CFTR vector transduced cells, we were able to detect CFTR mRNA expression using qPCR and function correction using fluorometric image plate reader (FLIPR) and iodide efflux assays. Iodides 166-172 CF transmembrane conductance regulator Homo sapiens 7-11 30969810-2 2019 Here, we compare the impact of the most common CF mutation F508del on the function of human and mouse CFTR heterologously expressed in mammalian cells and their response to CFTR modulators using the iodide efflux and patch-clamp techniques. Iodides 199-205 CF transmembrane conductance regulator Homo sapiens 173-177 30969810-5 2019 However, they all produced CFTR-mediated iodide efflux with human F508del-CFTR-expressing CHO cells, while fifteen CFTR correctors rescued the plasma membrane expression of both human and mouse F508del-CFTR. Iodides 41-47 CF transmembrane conductance regulator Homo sapiens 27-31 30969810-5 2019 However, they all produced CFTR-mediated iodide efflux with human F508del-CFTR-expressing CHO cells, while fifteen CFTR correctors rescued the plasma membrane expression of both human and mouse F508del-CFTR. Iodides 41-47 CF transmembrane conductance regulator Homo sapiens 74-78 30969810-6 2019 Interestingly, the CFTR potentiator genistein enhanced CFTR-mediated iodide efflux from CHO cells expressing either human or mouse F508del-CFTR, whereas it only potentiated human F508del-CFTR Cl- channels in cell-free membrane patches, suggesting that its action on mouse F508del-CFTR is indirect. Iodides 69-75 CF transmembrane conductance regulator Homo sapiens 55-59 22038833-9 2012 Ablation of K8 expression by siRNA in F508del-expressing HeLa cells led to the recovery of CFTR-dependent iodide efflux. Iodides 106-112 CF transmembrane conductance regulator Homo sapiens 91-95 26079370-3 2015 In Baby Hamster Kidney (BHK) cells expressing wild-type human CFTR, S1P (1mumol/L) attenuates forskolin-stimulated, CFTR-dependent iodide efflux. Iodides 131-137 CF transmembrane conductance regulator Homo sapiens 62-66 26079370-3 2015 In Baby Hamster Kidney (BHK) cells expressing wild-type human CFTR, S1P (1mumol/L) attenuates forskolin-stimulated, CFTR-dependent iodide efflux. Iodides 131-137 CF transmembrane conductance regulator Homo sapiens 116-120 23463616-2 2013 CFTR-dependent iodide transport measured by fluorescent quenching of ectopically expressed halide-sensitive yellow fluorescent protein (YFP) is widely being used to study CFTR function by microscopy or plate readers. Iodides 15-21 CF transmembrane conductance regulator Homo sapiens 0-4 23463616-2 2013 CFTR-dependent iodide transport measured by fluorescent quenching of ectopically expressed halide-sensitive yellow fluorescent protein (YFP) is widely being used to study CFTR function by microscopy or plate readers. Iodides 15-21 CF transmembrane conductance regulator Homo sapiens 171-175 22362924-4 2012 Using iodide efflux as a biochemical marker of CFTR activity and short circuit current (I(sc)) recordings, we found that the H2O2-stimulated efflux was abolished by cyclooxygenase-1 inhibition and potentially also involves microsomal prostaglandin E synthase-1 activity, implicating a role for PGE2 production. Iodides 6-12 CF transmembrane conductance regulator Homo sapiens 47-51 29172645-7 2017 The assay utilizes a cystic fibrosis bronchial epithelial (CFBE41o-) cell line, which was engineered to report CFTR-mediated intracellular flux of iodide by a halide-sensitive yellow fluorescence protein (YFP) reporter. Iodides 147-153 CF transmembrane conductance regulator Homo sapiens 111-115 28003367-7 2017 Intrinsic tryptophan fluorescence studies of CFTR showed that phosphorylation reduced iodide-mediated quenching, consistent with an effect of phosphorylation in burying tryptophans at the transmission interface. Iodides 86-92 CF transmembrane conductance regulator Homo sapiens 45-49 24788249-8 2014 Furthermore, iodide efflux assays confirmed that NHERF1 and P-ERM are necessary for VIP regulation of the stability and sustained activity of membrane CFTR. Iodides 13-19 CF transmembrane conductance regulator Homo sapiens 151-155 24338365-3 2014 Here, we used digital holographic microscopy (DHM) an interferometric technique to quantify in situ the transmembrane water fluxes during the activity of the epithelial chloride channel, CFTR, measured by patch-clamp and iodide efflux techniques. Iodides 221-227 CF transmembrane conductance regulator Homo sapiens 187-191 23984517-2 2013 CFTR-mediated iodide influx assay and patch-clamp tests were done on FRT cells stably co-transfected with human CFTR and EYFP/H148Q. Iodides 14-20 CF transmembrane conductance regulator Homo sapiens 0-4 21411725-4 2011 Iodide effluxes were used to monitor the presence of VIP-rescued functional F508del-CFTR channels at the surface of JME/CF15 cells maintained at 37 C. Iodide efflux peaks measured in response to stimulation with forskolin were insensitive to PKC alpha, beta, gamma, delta, zeta inhibitors. Iodides 151-157 CF transmembrane conductance regulator Homo sapiens 84-88 23029546-7 2012 Further, hAECs induced to express CFTR possessed functional iodide/chloride (I(-/)Cl(-)) ion channels that were inhibited by the CFTR-inhibitor CFTR-172, indicating the presence of functional CFTR ion channels. Iodides 60-66 CF transmembrane conductance regulator Homo sapiens 34-38 23029546-7 2012 Further, hAECs induced to express CFTR possessed functional iodide/chloride (I(-/)Cl(-)) ion channels that were inhibited by the CFTR-inhibitor CFTR-172, indicating the presence of functional CFTR ion channels. Iodides 60-66 CF transmembrane conductance regulator Homo sapiens 129-133 23029546-7 2012 Further, hAECs induced to express CFTR possessed functional iodide/chloride (I(-/)Cl(-)) ion channels that were inhibited by the CFTR-inhibitor CFTR-172, indicating the presence of functional CFTR ion channels. Iodides 60-66 CF transmembrane conductance regulator Homo sapiens 129-133 23029546-7 2012 Further, hAECs induced to express CFTR possessed functional iodide/chloride (I(-/)Cl(-)) ion channels that were inhibited by the CFTR-inhibitor CFTR-172, indicating the presence of functional CFTR ion channels. Iodides 60-66 CF transmembrane conductance regulator Homo sapiens 129-133 21708286-8 2011 We studied the CFTR maturation process using Western blot analysis and evaluated CFTR channel activity by automated iodide efflux assays. Iodides 116-122 CF transmembrane conductance regulator Homo sapiens 81-85 22074181-6 2011 Using iodide efflux assays, the probe molecules have been demonstrated to modulate the activity of mutant CFTR in the same manner as 1. Iodides 6-12 CF transmembrane conductance regulator Homo sapiens 106-110 21411725-4 2011 Iodide effluxes were used to monitor the presence of VIP-rescued functional F508del-CFTR channels at the surface of JME/CF15 cells maintained at 37 C. Iodide efflux peaks measured in response to stimulation with forskolin were insensitive to PKC alpha, beta, gamma, delta, zeta inhibitors. Iodides 0-6 CF transmembrane conductance regulator Homo sapiens 84-88 20110398-4 2010 Here, we present the effect of GPact-11a on CFTR activity using in vitro (iodide efflux, fluorescence imaging and patch-clamp recordings), ex vivo (short-circuit current measurements) and in vivo (salivary secretion) experiments. Iodides 74-80 CF transmembrane conductance regulator Homo sapiens 44-48 21594775-4 2011 CFTR activity can be simply assessed by measuring the rate of YFP signal decrease caused by iodide influx. Iodides 92-98 CF transmembrane conductance regulator Homo sapiens 0-4 21050066-0 2010 Optimization of a Yellow fluorescent protein-based iodide influx high-throughput screening assay for cystic fibrosis transmembrane conductance regulator (CFTR) modulators. Iodides 51-57 CF transmembrane conductance regulator Homo sapiens 101-152 21050066-0 2010 Optimization of a Yellow fluorescent protein-based iodide influx high-throughput screening assay for cystic fibrosis transmembrane conductance regulator (CFTR) modulators. Iodides 51-57 CF transmembrane conductance regulator Homo sapiens 154-158 20517942-9 2010 We observed activation of CFTR Cl(-) channels with iodide efflux, on addition of forskolin, 3-isobutyl-1-methyl-xanthine, and 8-chlorphenylthio-cyclic adenosine monophosphate, in wild-type C57BL/6J isolated muscle fibers in contrast to no efflux from mutant F508del-CFTR muscle. Iodides 51-57 CF transmembrane conductance regulator Homo sapiens 26-30 20517942-9 2010 We observed activation of CFTR Cl(-) channels with iodide efflux, on addition of forskolin, 3-isobutyl-1-methyl-xanthine, and 8-chlorphenylthio-cyclic adenosine monophosphate, in wild-type C57BL/6J isolated muscle fibers in contrast to no efflux from mutant F508del-CFTR muscle. Iodides 51-57 CF transmembrane conductance regulator Homo sapiens 266-270 20089668-8 2010 As CFTR transports chloride as well as other halides, we conjugated an iodide-sensitive probe as an independent approach to confirm the results. Iodides 71-77 CF transmembrane conductance regulator Homo sapiens 3-7 20089668-10 2010 CFTR(inh)172 blocked 40-50% of the overall iodide uptake by phagosomes in normal neutrophils. Iodides 43-49 CF transmembrane conductance regulator Homo sapiens 0-4 19350818-2 2009 A stably transfected Fischer rat thyroid (FRT) epithelial cell lines co-expressing human CFTR and a green fluorescent protein mutant with ultra-high halide sensitivity (EYFP) were used to measure CFTR-mediated iodide influx rates. Iodides 210-216 CF transmembrane conductance regulator Homo sapiens 196-200 19774621-6 2009 METHODS: Molecular characterization of S1118F-CFTR mutant was studied in HEK-293 cells at 37 degrees C. Various biochemical methods such as Western blotting, real-time PCR, Pulse chase labeling and iodide efflux assay were employed. Iodides 198-204 CF transmembrane conductance regulator Homo sapiens 46-50 19584307-5 2009 One hour of treatment with VIP strongly increased F508del-CFTR activity, with iodide efflux peaks three times higher than with untreated cells. Iodides 78-84 CF transmembrane conductance regulator Homo sapiens 58-62 19584307-6 2009 At 37 degrees C, VIP-treated cells, but not untreated controls, showed significant iodide efflux peaks that were sensitive to the CFTR inhibitor 3-[(3-trifluoromethyl)phenyl]-5-[(4-carboxyphenyl)methylene]-2-thioxo-4-thiazolidinone (CFTR(inh)-172). Iodides 83-89 CF transmembrane conductance regulator Homo sapiens 130-134 16127463-3 2005 To identify small-molecule correctors of defective cellular processing, we assayed iodide flux in DeltaF508-CFTR-transfected epithelial cells using a fluorescent halide indicator. Iodides 83-89 CF transmembrane conductance regulator Homo sapiens 108-112 17654517-5 2008 The presence of functional CFTR was confirmed using iodide efflux assay. Iodides 52-58 CF transmembrane conductance regulator Homo sapiens 27-31 16604470-2 2005 Here, we studied the contribution of the conserved residues G551 and G1349 to the pharmacological modulation of CFTR chloride channels by phloxine B using iodide efflux and whole-cell patch clamp experiments performed on the following green fluorescent protein (GFP)-tagged CFTR: wild-type, delF508, G551D, G1349D, and G551D/G1349D double mutant. Iodides 155-161 CF transmembrane conductance regulator Homo sapiens 112-116 17690331-7 2008 The iodide efflux seen with 8-iso-PGE(2) is abolished by the EP(4) receptor antagonist AH23848, the CFTR inhibitor 172, and inhibition of PKA and the PI3K pathway. Iodides 4-10 CF transmembrane conductance regulator Homo sapiens 100-104 17898794-6 2007 We also confirmed the functional channel activity of GFP-CFTR in an iodide efflux assay. Iodides 68-74 CF transmembrane conductance regulator Homo sapiens 57-61 11262417-3 2001 The compounds were screened for their ability to activate CFTR at 50 microm concentration by measurement of the kinetics of iodide influx in Fisher rat thyroid cells expressing wild-type or G551D CFTR together with the green fluorescent protein-based halide indicator YFP-H148Q. Iodides 124-130 CF transmembrane conductance regulator Homo sapiens 58-62 16196493-6 2005 Incubation of BHK cells stably expressing human DeltaF508 CFTR with 1-10 microM CF(cor)-325 resulted in maturation and delivery of a functional molecule to the cell surface as determined by the iodide efflux assay. Iodides 194-200 CF transmembrane conductance regulator Homo sapiens 58-62 12588899-6 2003 Stimulation of iodide efflux by chlorophenylthio-cAMP (cpt-cAMP) was delayed in cells expressing 9CA channels, and a similar delay was observed when cells expressing wild-type CFTR were treated with the PKC inhibitor chelerythrine. Iodides 15-21 CF transmembrane conductance regulator Homo sapiens 176-180 12397014-7 2002 We report that H146, H345, and H727 express CFTR messenger RNA (reverse transcription polymerase chain reaction) and protein (immunoblotting) and possess functional CFTR Cl(-) conductance, demonstrated by an iodide efflux assay inhibitable by transfection with antisense CFTR. Iodides 208-214 CF transmembrane conductance regulator Homo sapiens 165-169 12397014-7 2002 We report that H146, H345, and H727 express CFTR messenger RNA (reverse transcription polymerase chain reaction) and protein (immunoblotting) and possess functional CFTR Cl(-) conductance, demonstrated by an iodide efflux assay inhibitable by transfection with antisense CFTR. Iodides 208-214 CF transmembrane conductance regulator Homo sapiens 165-169 10564094-0 1999 Long-wavelength iodide-sensitive fluorescent indicators for measurement of functional CFTR expression in cells. Iodides 16-22 CF transmembrane conductance regulator Homo sapiens 86-90 1712984-3 1991 The sequence of anion selectivity of cAMP-regulated channels in cells containing either endogenous or recombinant CFTR was bromide greater than chloride greater than iodide greater than fluoride. Iodides 166-172 CF transmembrane conductance regulator Homo sapiens 114-118 9305991-4 1997 When properly processed mutants were evaluated for functional defects by the iodide efflux method, the G178R- and E193K-CFTR-expressing cell lines showed impaired anion translocation activities. Iodides 77-83 CF transmembrane conductance regulator Homo sapiens 120-124 1282304-5 1992 Cell-attached and outside-out patches from confluent CFTR+ but not CFTR- cells revealed 6-pS channels having linear current-voltage relations, permselectivity Cl > I (partial block by external iodide), and little or no inhibition by 5-nitro-2-(3-phenylpropylamino)-benzoate. Iodides 196-202 CF transmembrane conductance regulator Homo sapiens 53-57