PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8804611-2 1996 Here we have used SPR spectroscopy for the first time to monitor the binding and activation of G-protein (transducin or Gt) by bovine rhodopsin incorporated into an egg phosphatidylcholine bilayer deposited on a silver film. Phosphatidylcholines 169-188 rhodopsin Bos taurus 134-143 9726932-5 1998 Reconstitution of rhodopsin into phosphatidylcholine vesicles has little influence on the spectral properties of the rhodopsin-transducin complex, whereas pH affects the intensity of the carbonyl stretching band. Phosphatidylcholines 33-52 rhodopsin Bos taurus 18-27 7947780-1 1994 Surface plasmon resonance (SPR) spectroscopy has been used to follow incorporation and light-induced conformational changes in bovine rhodopsin reconstituted into an egg phosphatidylcholine bilayer deposited on a thin silver film. Phosphatidylcholines 170-189 rhodopsin Bos taurus 134-143 8554325-6 1995 The lipid which tended to be associated with rhodopsin in protein-lipid-detergent mixed micelles was also consistently richer in PC than that present in lipid-detergent micelles. Phosphatidylcholines 129-131 rhodopsin Bos taurus 45-54 8384876-5 1993 This reduction in rotational mobility is also found with purified squid rhodopsin reconstituted in egg phosphatidylcholine and in urea-washed outer segment membranes which have been treated with endoprotease-V8 to remove the C-terminal extension of squid rhodopsin. Phosphatidylcholines 103-122 rhodopsin Bos taurus 72-81 3207703-0 1988 Equilibrium and dynamic bilayer structural properties of unsaturated acyl chain phosphatidylcholine-cholesterol-rhodopsin recombinant vesicles and rod outer segment disk membranes as determined from higher order analysis of fluorescence anisotropy decay. Phosphatidylcholines 80-99 rhodopsin Bos taurus 112-121 2140896-1 1990 Expression of functionally active bovine visual rhodopsin was achieved by sequential transcription and translation in vitro of rhodopsin gene cDNA with co-translational insertion of the protein into phosphatidylcholine liposomes. Phosphatidylcholines 199-218 rhodopsin Bos taurus 48-57 2357235-3 1990 Functional expression of bovine visual rhodopsin in the cell-free translation system with cotranslational insertion of the protein into phosphatidylcholine liposomes is described. Phosphatidylcholines 136-155 rhodopsin Bos taurus 39-48 3050446-1 1988 Rhodopsin kinase was purified from bovine retina rod outer segments as a 62-64-kDa protein that phosphorylated purified rhodopsin reconstituted into egg phosphatidylcholine/phosphatidylethanolamine liposomes. Phosphatidylcholines 153-172 rhodopsin Bos taurus 120-129 3365383-4 1988 After hexane extraction of lyophilized disks, PC is the major component of the fraction of lipids that remains associated with rhodopsin, followed by phosphatidylserine, while a large proportion of the phosphatidylethanolamine is removed. Phosphatidylcholines 46-48 rhodopsin Bos taurus 127-136 3365383-7 1988 VLCPUFA-containing PCs are the most highly concentrated species in the rhodopsin-associated lipid fraction. Phosphatidylcholines 19-22 rhodopsin Bos taurus 71-80 6243483-16 1980 The differences in the results obtained with the various phosphatidylcholines above their transition temperature suggest that the solubility of rhodopsin in bilayers depends not only on the fluidity of the lipids, but also, to some extent, on the phospholipid chain length. Phosphatidylcholines 57-77 rhodopsin Bos taurus 144-153 3838249-3 1985 As part of the characterization of this preparation, the surface orientation of the carbohydrates of rhodopsin, assembled from purified bovine rhodopsin and egg phosphatidylcholine was examined, and is the topic of this report. Phosphatidylcholines 161-180 rhodopsin Bos taurus 101-110 7407117-5 1980 50 mol of phosphatidylcholine were maximally bound to 1 mol rhodopsin when the purified rhodopsin was mixed with phosphatidylcholine in 0.5% cholate. Phosphatidylcholines 10-29 rhodopsin Bos taurus 60-69 7407117-5 1980 50 mol of phosphatidylcholine were maximally bound to 1 mol rhodopsin when the purified rhodopsin was mixed with phosphatidylcholine in 0.5% cholate. Phosphatidylcholines 10-29 rhodopsin Bos taurus 88-97 7407117-5 1980 50 mol of phosphatidylcholine were maximally bound to 1 mol rhodopsin when the purified rhodopsin was mixed with phosphatidylcholine in 0.5% cholate. Phosphatidylcholines 113-132 rhodopsin Bos taurus 60-69 3881128-0 1985 Rhodopsin-egg phosphatidylcholine reconstitution by an octyl glucoside dilution procedure. Phosphatidylcholines 14-33 rhodopsin Bos taurus 0-9 3881128-1 1985 The transmembrane protein bovine rhodopsin was reconstituted with egg phosphatidylcholine (PC) by using a modified detergent dilution technique employing the nonionic detergent octyl-beta-D-glucoside (octyl glucoside). Phosphatidylcholines 70-89 rhodopsin Bos taurus 33-42 6275924-5 1982 For the phosphatidylcholine spin label there are effectively 55 +/- 5 lipids/200,000-dalton cytochrome oxidase, 58 +/- 4 mol lipid/265,000 dalton (Na+, K+)-ATPase, and 24 +/- 3 and 22 +/- 2 mol lipid/37,000 dalton rhodopsin for the bovine and frog preparations, respectively. Phosphatidylcholines 8-27 rhodopsin Bos taurus 214-223 949475-6 1976 Bilayers prepared from extracted disk lipids had a microviscosity which was about one-fourth that of the intact disk membrane, demonstrating that rhodopsin hinders the mobility of the hydrocarbon chains of the disk phospholipids or egg phosphatidylcholine had identical microviscosities despite the much higher degree of unsaturation of the disk phospholipids. Phosphatidylcholines 236-255 rhodopsin Bos taurus 146-155