PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9202883-2 1997 PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGa) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA). Phosphatidylcholines 221-240 phospholipase A2 group IB Homo sapiens 0-4 9032461-5 1997 Kinetic analysis of recombinant hs-PLA2 demonstrates that hs-PLA2 strongly prefers PA as substrate over other phospholipids found in the mammalian plasma membrane including phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Phosphatidylcholines 198-217 phospholipase A2 group IB Homo sapiens 32-39 9032461-5 1997 Kinetic analysis of recombinant hs-PLA2 demonstrates that hs-PLA2 strongly prefers PA as substrate over other phospholipids found in the mammalian plasma membrane including phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Phosphatidylcholines 198-217 phospholipase A2 group IB Homo sapiens 58-65 9032461-5 1997 Kinetic analysis of recombinant hs-PLA2 demonstrates that hs-PLA2 strongly prefers PA as substrate over other phospholipids found in the mammalian plasma membrane including phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Phosphatidylcholines 219-221 phospholipase A2 group IB Homo sapiens 32-39 9032461-5 1997 Kinetic analysis of recombinant hs-PLA2 demonstrates that hs-PLA2 strongly prefers PA as substrate over other phospholipids found in the mammalian plasma membrane including phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Phosphatidylcholines 219-221 phospholipase A2 group IB Homo sapiens 58-65 9032461-9 1997 Thus it appears that Lys-69 is at least partially involved in the PA specificity of hs-PLA2 and Glu-56 in the distinction between PE and PC. Phosphatidylcholines 137-139 phospholipase A2 group IB Homo sapiens 84-91 9297603-6 1997 PLA caused a small but significant release of radiolabel from TDI/PCL/ED which was enhanced in the presence of its substrate, PC, and a mixture of PC with its breakdown products, LPC and OA. Phosphatidylcholines 66-68 phospholipase A2 group IB Homo sapiens 0-3 9297603-6 1997 PLA caused a small but significant release of radiolabel from TDI/PCL/ED which was enhanced in the presence of its substrate, PC, and a mixture of PC with its breakdown products, LPC and OA. Phosphatidylcholines 126-128 phospholipase A2 group IB Homo sapiens 0-3 8960386-2 1996 In the present study, we investigated the susceptibility of the phosphatidylcholine component of two exogenous surfactants, Exosurf and Survanta, to secretory-type phospholipase A2 (PLA2) deacylation in vitro. Phosphatidylcholines 64-83 phospholipase A2 group IB Homo sapiens 164-180 9454375-0 1997 [Effects of fatty acid amides on phosphatidylcholine hydrolysis catalyzed by phospholipase A2 in micelles]. Phosphatidylcholines 33-52 phospholipase A2 group IB Homo sapiens 77-93 9454375-1 1997 The effect of fatty acid amides on the hydrolysis of natural phosphatidylcholine and its semisynthetic analog, dioleoyl phosphatidylcholine, catalyzed by phospholipase A2 in mixed micelles with Triton X-100 has been studied. Phosphatidylcholines 61-80 phospholipase A2 group IB Homo sapiens 154-170 8944749-9 1996 A novel method for continuous assay of phospholipase A2 activity with BSA-HCA and a mixed phosphatidylcholine/CHAPS micellar substrate is reported. Phosphatidylcholines 90-109 phospholipase A2 group IB Homo sapiens 39-55 8824283-8 1996 All glycosaminoglycans tested, at concentrations up to 100 microM, increased the activity of phospholipase A2 toward phosphatidylcholine liposomes. Phosphatidylcholines 117-136 phospholipase A2 group IB Homo sapiens 93-109 8960386-2 1996 In the present study, we investigated the susceptibility of the phosphatidylcholine component of two exogenous surfactants, Exosurf and Survanta, to secretory-type phospholipase A2 (PLA2) deacylation in vitro. Phosphatidylcholines 64-83 phospholipase A2 group IB Homo sapiens 182-186 8960386-4 1996 The phosphatidylcholine component of Survanta was readily deacylated by PLA2, whereas the dipalmitoylphosphatidycholine (DPPC) component of Exosurf was resistant over the entire duration of the assay. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 72-76 8847457-12 1996 Phosphatidylcholine containing [14C]-AA in Sn-2 (150,000 dpm) was incubated with cytosol of uterine myometrium and the amounts of [14C]-AA released were calculated as Phospholipase A2 activity. Phosphatidylcholines 0-19 phospholipase A2 group IB Homo sapiens 167-183 8792123-4 1996 BALF PLA2 showed marked selectivity for phosphatidylcholine containing arachidonic acid (AA) over linoleic or palmitic acids. Phosphatidylcholines 40-59 phospholipase A2 group IB Homo sapiens 5-9 8679568-8 1996 Endogenous PC in the insect cells fulfills the lipid requirement for the expressed BDH since enzymatic activity is lost upon digestion with phospholipase A2 and restored selectively by reconstitution with PC vesicles. Phosphatidylcholines 11-13 phospholipase A2 group IB Homo sapiens 140-156 11854811-5 1995 We observed that IL-14 induces phospholipase A(2) (PLA(2))-dependent release of arachidonic acid from phosphatidylcholine and phosphatidylinositol. Phosphatidylcholines 102-121 phospholipase A2 group IB Homo sapiens 31-49 8664287-1 1996 Bilayers composed of phosphatidylcholine initially resist catalysis by phospholipase A2. Phosphatidylcholines 21-40 phospholipase A2 group IB Homo sapiens 71-87 8867924-7 1996 fMLP, but not tBuBHQ, caused BAPTA/AM-sensitive activation of phospholipase A2 and D. tBuBHQ caused O2- production by interacting with phosphatidylcholine in a cell-free system. Phosphatidylcholines 135-154 phospholipase A2 group IB Homo sapiens 62-78 11854811-5 1995 We observed that IL-14 induces phospholipase A(2) (PLA(2))-dependent release of arachidonic acid from phosphatidylcholine and phosphatidylinositol. Phosphatidylcholines 102-121 phospholipase A2 group IB Homo sapiens 51-57 8578536-9 1995 Addition of pancreatic phospholipase A2 (PLA2) in doses sufficient to hydrolyze more than 95% of the phosphatidylethanolamine (PE) and 37% of the phosphatidylcholine (PC) decreased the binding by 50%. Phosphatidylcholines 146-165 phospholipase A2 group IB Homo sapiens 23-39 8578536-9 1995 Addition of pancreatic phospholipase A2 (PLA2) in doses sufficient to hydrolyze more than 95% of the phosphatidylethanolamine (PE) and 37% of the phosphatidylcholine (PC) decreased the binding by 50%. Phosphatidylcholines 146-165 phospholipase A2 group IB Homo sapiens 41-45 8578536-9 1995 Addition of pancreatic phospholipase A2 (PLA2) in doses sufficient to hydrolyze more than 95% of the phosphatidylethanolamine (PE) and 37% of the phosphatidylcholine (PC) decreased the binding by 50%. Phosphatidylcholines 167-169 phospholipase A2 group IB Homo sapiens 23-39 8578536-9 1995 Addition of pancreatic phospholipase A2 (PLA2) in doses sufficient to hydrolyze more than 95% of the phosphatidylethanolamine (PE) and 37% of the phosphatidylcholine (PC) decreased the binding by 50%. Phosphatidylcholines 167-169 phospholipase A2 group IB Homo sapiens 41-45 8578536-10 1995 Doses of PLA2 that hydrolyze more than 95% of the phosphatidylethanolamine (PE) and 37% of the phosphatidylcholine (PC) decreased the binding by 50%. Phosphatidylcholines 95-114 phospholipase A2 group IB Homo sapiens 9-13 8578536-10 1995 Doses of PLA2 that hydrolyze more than 95% of the phosphatidylethanolamine (PE) and 37% of the phosphatidylcholine (PC) decreased the binding by 50%. Phosphatidylcholines 116-118 phospholipase A2 group IB Homo sapiens 9-13 7631805-9 1995 This form of PLA2 exhibited a neutral and broad pH optimum (pH 6.0-8.0) and hydrolyzed both phosphatidylethanolamine and phosphatidylcholine effectively. Phosphatidylcholines 121-140 phospholipase A2 group IB Homo sapiens 13-17 7857976-1 1995 The rate of hydrolysis of phosphatidylcholine bilayers by soluble phospholipase A2 (PLA2) is greatly enhanced by the presence in the bilayer of a threshold mole fraction of the reaction products: fatty acid and lysophosphatidylcholine (lyso-PC). Phosphatidylcholines 26-45 phospholipase A2 group IB Homo sapiens 84-88 8846880-3 1995 The results showed that CT Vc5 inhibits PLA2 activity on phosphatidylcholine liposomes. Phosphatidylcholines 57-76 phospholipase A2 group IB Homo sapiens 40-44 7857976-1 1995 The rate of hydrolysis of phosphatidylcholine bilayers by soluble phospholipase A2 (PLA2) is greatly enhanced by the presence in the bilayer of a threshold mole fraction of the reaction products: fatty acid and lysophosphatidylcholine (lyso-PC). Phosphatidylcholines 26-45 phospholipase A2 group IB Homo sapiens 66-82 7895399-4 1994 PLA2 activity was measured in lysates of the various cell fractions by the hydrolysis of radiolabeled lysocholine from phosphatidylcholine, L-Dipalmitoyl using thin layer chromatography. Phosphatidylcholines 119-138 phospholipase A2 group IB Homo sapiens 0-4 7751812-13 1995 The use of a fluorescent dialkyl- instead of diacyl-glycerophosphocholine for transfer studies was mandatory, as we found that lipoproteins contained phospholipase A2 activity toward long-chain phosphatidylcholine. Phosphatidylcholines 194-213 phospholipase A2 group IB Homo sapiens 150-166 7893853-2 1995 In cholinergic neurons PLA2 controls the physico-chemical properties of neuronal membranes as well as the breakdown of phosphatidylcholine to produce choline for acetylcholine synthesis. Phosphatidylcholines 119-138 phospholipase A2 group IB Homo sapiens 23-27 7964477-4 1994 CD69 cross-linking resulted also in phospholipase A2 activation, as detected by in vivo arachidonic acid release measurement from intact cells and by direct in vitro measurement of enzymatic activity using radiolabeled phosphatidylcholine vesicles. Phosphatidylcholines 219-238 phospholipase A2 group IB Homo sapiens 36-52 8514863-4 1993 The major cytosolic phospholipase A2 isoform preferentially hydrolyzes plasmalogen substrate, possesses a pH optimum of 7.0, and is chromatographically resolvable from a minor cytosolic calcium-independent phospholipase A2 isoform that hydrolyzes plasmenylcholine and phosphatidylcholine substrates at similar rates and possesses a pH optimum of 8.5. Phosphatidylcholines 268-287 phospholipase A2 group IB Homo sapiens 20-36 8063019-4 1994 PLA2 activity in sonicates from ram spermatozoa was enhanced when 1-stearoyl-2-arachidonoyl-sn-glycerol, the diacylglycerol usually generated by polyphosphoinositide breakdown, was added to a radioactive phosphatidylcholine substrate; the effect was time- and Ca(2+)-dependent. Phosphatidylcholines 204-223 phospholipase A2 group IB Homo sapiens 0-4 8373761-0 1993 Polymerizable phosphatidylcholines: importance of phospholipid motions for optimum phospholipase A2 and C activity. Phosphatidylcholines 14-34 phospholipase A2 group IB Homo sapiens 83-99 7890144-4 1994 Both membrane-active peptides, cardiotoxin and thionin, inhibited the PLA2 activity on phosphatidylcholine liposomes. Phosphatidylcholines 87-106 phospholipase A2 group IB Homo sapiens 70-74 7864659-9 1994 The results demonstrated that lysoPC, a phospholipase A2-generated hydrolysis product of phosphatidylcholine, induced T-lymphocyte chemotaxis in vitro. Phosphatidylcholines 89-108 phospholipase A2 group IB Homo sapiens 40-56 7864659-10 1994 Because phosphatidylcholine is the major phospholipid in the epidermis, the activation of phospholipase A2 may result in the release of lysoPC in concentrations capable of inducing migration of T lymphocytes into the epidermis. Phosphatidylcholines 8-27 phospholipase A2 group IB Homo sapiens 90-106 8128456-9 1993 Secreted phospholipase A2 hydrolyzed phosphatidylethanolamine at 5-12 times the rate of phosphatidylcholine when the substrates were present in pure form. Phosphatidylcholines 88-107 phospholipase A2 group IB Homo sapiens 9-25 8292693-1 1993 We measured serum phospholipase A2 (PLA2) activity in 39 schizophrenics, 26 psychiatric controls, and 26 normal controls using a radioenzymatic assay with phosphatidylcholine as precursor. Phosphatidylcholines 155-174 phospholipase A2 group IB Homo sapiens 18-34 8292693-1 1993 We measured serum phospholipase A2 (PLA2) activity in 39 schizophrenics, 26 psychiatric controls, and 26 normal controls using a radioenzymatic assay with phosphatidylcholine as precursor. Phosphatidylcholines 155-174 phospholipase A2 group IB Homo sapiens 36-40 7508272-10 1993 The human serum phospholipase A2 strongly preferred E. coli membranes as substrate to the mixed micelles containing phosphatidylcholine/phosphatidylethanolamine. Phosphatidylcholines 116-135 phospholipase A2 group IB Homo sapiens 16-32 8264152-2 1993 In the kidney, Ang II at nanomolar concentration binds to proximal tubular cells and stimulates phospholipase A2 (PLA2), which in turn catalyzes the hydrolysis of phosphatidylcholine into lysophosphatidylcholine (LPC) and fatty acid. Phosphatidylcholines 163-182 phospholipase A2 group IB Homo sapiens 96-112 8264152-2 1993 In the kidney, Ang II at nanomolar concentration binds to proximal tubular cells and stimulates phospholipase A2 (PLA2), which in turn catalyzes the hydrolysis of phosphatidylcholine into lysophosphatidylcholine (LPC) and fatty acid. Phosphatidylcholines 163-182 phospholipase A2 group IB Homo sapiens 114-118 8099446-1 1993 2-Lysophosphatidylcholine and cis-unsaturated fatty acids such as linoleic and linolenic acids, which are the products of the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2 (EC 3.1.1.4), significantly potentiate the differentiation of HL-60 cells to macrophages that is induced by either a membrane-permeant diacylglycerol or a phorbol ester. Phosphatidylcholines 6-25 phospholipase A2 group IB Homo sapiens 173-189 8316043-0 1993 Phosphatidylcholine as substrate for human pancreatic phospholipase A2. Phosphatidylcholines 0-19 phospholipase A2 group IB Homo sapiens 54-70 8388718-7 1993 For comparison we also studied the effects of sphingosine on the phospholipase A2 catalyzed hydrolysis of the pyrene-labeled acidic alkylacyl phospholipid analog 1-octacosanyl-2-[6-(pyren-1-yl)]hexanoyl-sn-glycero-3- phosphatidylmethanol (C28-O-PHPM) and the corresponding phosphatidylcholine (C28-O-PHPC). Phosphatidylcholines 273-292 phospholipase A2 group IB Homo sapiens 65-81 8504138-1 1993 The activity of soluble phospholipase A2 to hydrolyze phosphatidylcholine vesicles increases abruptly after a lag time of several minutes. Phosphatidylcholines 54-73 phospholipase A2 group IB Homo sapiens 24-40 8316043-2 1993 The long-chain phosphatidylcholine/sodium cholate aqueous system as substrate for human pancreatic phospholipase A2 (PLA2) was investigated. Phosphatidylcholines 15-34 phospholipase A2 group IB Homo sapiens 99-115 8316043-2 1993 The long-chain phosphatidylcholine/sodium cholate aqueous system as substrate for human pancreatic phospholipase A2 (PLA2) was investigated. Phosphatidylcholines 15-34 phospholipase A2 group IB Homo sapiens 117-121 8316043-10 1993 Phosphatidylcholine was preferred as substrate for human PLA2 when present in large mixed disc-like bile salt micelles. Phosphatidylcholines 0-19 phospholipase A2 group IB Homo sapiens 57-61 1526278-8 1992 In parallel, and with the same time-dependent effect, a significant decrease in phosphatidylcholine labeling was observed in IFN-gamma-treated cells, further indicating that a potential signal transduction mechanism of IFN-gamma is the hydrolysis of membrane phosphatidylcholine by phospholipase A2. Phosphatidylcholines 80-99 phospholipase A2 group IB Homo sapiens 282-298 1451798-3 1992 We demonstrate here that membrane cholesterol initiates the activation of phosphatidyl choline phospholipase D and phosphatidic acid thus generated promotes the activation of its phospholipase A2 in the presence of extraplatelet calcium. Phosphatidylcholines 74-94 phospholipase A2 group IB Homo sapiens 179-195 1334462-0 1992 Macrophage colony stimulating factor activates phosphatidylcholine hydrolysis by cytoplasmic phospholipase A2. Phosphatidylcholines 47-66 phospholipase A2 group IB Homo sapiens 93-109 8444905-6 1993 Activation of endogenous phospholipase A2 by mellitin in vivo or hydrolysis of phosphatidylcholine by purified phospholipase A2 in vitro inhibited PDGF receptor autophosphorylation similar to that of purified fatty acids. Phosphatidylcholines 79-98 phospholipase A2 group IB Homo sapiens 111-127 8431204-10 1993 Addition of quinacrine was also demonstrated to abolish the IL-1-induced hydrolysis of PC and PE but not PI, indicating that PC and PE are the preferred substrates for PLA2 enzymatic activity in human synovial cells. Phosphatidylcholines 87-89 phospholipase A2 group IB Homo sapiens 168-172 8431204-10 1993 Addition of quinacrine was also demonstrated to abolish the IL-1-induced hydrolysis of PC and PE but not PI, indicating that PC and PE are the preferred substrates for PLA2 enzymatic activity in human synovial cells. Phosphatidylcholines 125-127 phospholipase A2 group IB Homo sapiens 168-172 8431204-11 1993 CONCLUSION: Our findings strongly suggest that AA and PGE2 production by IL-1-triggered synoviocytes are largely dependent upon PLA2-mediated hydrolysis of PC and PE and to a lesser extent upon the earlier degradation of PI. Phosphatidylcholines 156-158 phospholipase A2 group IB Homo sapiens 128-132 8422369-1 1993 Phospholipase A2-catalyzed hydrolysis of phosphatidylcholine large unilamellar vesicles is characterized by a period of slow hydrolysis followed by a rapid increase in the rate of hydrolysis. Phosphatidylcholines 41-60 phospholipase A2 group IB Homo sapiens 0-16 8274032-3 1993 The isolated fractions of phosphatidylcholine (PC), phosphatidylinositol (PI) and phosphatidyl-ethanolamine (PE) were treated with phospholipase A2 to release fatty acids in the sn-2 position. Phosphatidylcholines 26-45 phospholipase A2 group IB Homo sapiens 131-147 1387325-5 1992 Despite a large excess of detergent, precipitates were, at times, observed upon incubation of some PC/DOC mixtures at 37 degrees C. Such behavior is of interest in view of the numerous reports of PLA2 inhibition by annexins and annexin-derived peptides in the PC/DOC system. Phosphatidylcholines 99-101 phospholipase A2 group IB Homo sapiens 196-200 1510717-3 1992 Cell stimulation results in the activation of phospholipase A2 (PLA2) whose major substrate is phosphatidylcholine (PC) and the release of the eicosanoid precursor arachidonic acid (AA) from PC. Phosphatidylcholines 95-114 phospholipase A2 group IB Homo sapiens 46-62 1510717-3 1992 Cell stimulation results in the activation of phospholipase A2 (PLA2) whose major substrate is phosphatidylcholine (PC) and the release of the eicosanoid precursor arachidonic acid (AA) from PC. Phosphatidylcholines 95-114 phospholipase A2 group IB Homo sapiens 64-68 1510717-3 1992 Cell stimulation results in the activation of phospholipase A2 (PLA2) whose major substrate is phosphatidylcholine (PC) and the release of the eicosanoid precursor arachidonic acid (AA) from PC. Phosphatidylcholines 116-118 phospholipase A2 group IB Homo sapiens 46-62 1510717-3 1992 Cell stimulation results in the activation of phospholipase A2 (PLA2) whose major substrate is phosphatidylcholine (PC) and the release of the eicosanoid precursor arachidonic acid (AA) from PC. Phosphatidylcholines 116-118 phospholipase A2 group IB Homo sapiens 64-68 1627547-0 1992 Basis for the anomalous effect of competitive inhibitors on the kinetics of hydrolysis of short-chain phosphatidylcholines by phospholipase A2. Phosphatidylcholines 102-122 phospholipase A2 group IB Homo sapiens 126-142 1631141-7 1992 The results suggest that cis-unsaturated fatty acids, which are presumably produced from phosphatidylcholine by signal-dependent activation of phospholipase A2, may take part directly in cell signaling through the protein kinase C pathway. Phosphatidylcholines 89-108 phospholipase A2 group IB Homo sapiens 143-159 1563569-1 1992 Phospholipase A2 activity (EC 3.1.1.4) was estimated in low-speed supernatants of human placenta by measuring the release of arachidonic acid from phosphatidylcholine, 1-stearoyl-2-[3H]arachidonyl and other phospholipids under alkaline conditions (pH 8). Phosphatidylcholines 147-166 phospholipase A2 group IB Homo sapiens 0-16 1315360-6 1992 Finally, direct measurement of enzymatic activity in vitro using radiolabeled phospholipid vesicles showed that CD69 cross-linking resulted in PLA2-dependent arachidonic acid and lysophosphatidylcholine generation from phosphatidylcholine, which was sensitive to quinacrine but not to R68070. Phosphatidylcholines 183-202 phospholipase A2 group IB Homo sapiens 143-147 1533163-1 1992 The selectivity of phospholipase A2 from serum was evaluated using radioassays and mass analyses of fatty acids liberated from phosphatidylcholine and phosphatidylethanolamine. Phosphatidylcholines 127-146 phospholipase A2 group IB Homo sapiens 19-35 1576162-4 1992 In recent years the fluorescent analogue of phosphatidylcholine, C6-NBD-PC, has been used for determination of the activity of soluble and membrane-bound PLA2. Phosphatidylcholines 44-63 phospholipase A2 group IB Homo sapiens 154-158 1537086-4 1992 In sharp contrast, human mitochondrial phospholipase A2 1) accounts for only a diminutive amount of total myocardial phospholipase A2 activity (1-2%), 2) is augmented by calcium ion, 3) exhibits a higher reaction velocity using phosphatidylcholine in comparison with plasmenylcholine substrate, and 4) is not substantially inhibited by either DTNB or bromoenol lactone. Phosphatidylcholines 228-247 phospholipase A2 group IB Homo sapiens 39-55 1787343-7 1991 Concomitantly, phospholipase A2 (PLA2) hydrolyzed PC producing a transiently increased level of lysophosphatidylcholine. Phosphatidylcholines 50-52 phospholipase A2 group IB Homo sapiens 15-31 1756867-6 1991 Phosphatidylcholine in mast cell membranes was appreciably hydrolyzed to liberate free arachidonic acid when mast cells were incubated with 14-kDa group II phospholipase A2 added exogenously in the presence of the antigen. Phosphatidylcholines 0-19 phospholipase A2 group IB Homo sapiens 156-172 1787343-7 1991 Concomitantly, phospholipase A2 (PLA2) hydrolyzed PC producing a transiently increased level of lysophosphatidylcholine. Phosphatidylcholines 50-52 phospholipase A2 group IB Homo sapiens 33-37 1991158-8 1991 These results suggest that an eicosanoid-independent degradation of phosphatidylethanolamine via phospholipase A2 at lower collagen levels may provide a source of the initial AA for conversion to TxA2 and the subsequent deacylation of phosphatidylinositol, phosphatidylcholine, and also phosphatidylserine. Phosphatidylcholines 257-276 phospholipase A2 group IB Homo sapiens 97-113 2038071-8 1991 In crude homogenates of myocardial tissue, the total enzymic activity for catabolism of LPC far exceeds the total activity for synthesis of LPC mediated by phospholipase A2 (PLA2) catalyzed hydrolysis of phosphatidylcholine (PC). Phosphatidylcholines 204-223 phospholipase A2 group IB Homo sapiens 156-172 2038071-8 1991 In crude homogenates of myocardial tissue, the total enzymic activity for catabolism of LPC far exceeds the total activity for synthesis of LPC mediated by phospholipase A2 (PLA2) catalyzed hydrolysis of phosphatidylcholine (PC). Phosphatidylcholines 204-223 phospholipase A2 group IB Homo sapiens 174-178 2038071-8 1991 In crude homogenates of myocardial tissue, the total enzymic activity for catabolism of LPC far exceeds the total activity for synthesis of LPC mediated by phospholipase A2 (PLA2) catalyzed hydrolysis of phosphatidylcholine (PC). Phosphatidylcholines 89-91 phospholipase A2 group IB Homo sapiens 156-172 2038071-8 1991 In crude homogenates of myocardial tissue, the total enzymic activity for catabolism of LPC far exceeds the total activity for synthesis of LPC mediated by phospholipase A2 (PLA2) catalyzed hydrolysis of phosphatidylcholine (PC). Phosphatidylcholines 89-91 phospholipase A2 group IB Homo sapiens 174-178 1654115-4 1991 These results show that phospholipase A2 was activated in hypoxic myocytes and had substrate specificity towards PC and PE. Phosphatidylcholines 113-115 phospholipase A2 group IB Homo sapiens 24-40 1872854-1 1991 An immediate reaction product of phosphatidylcholine hydrolysis catalyzed by phospholipase A2, lysophosphatidylcholine (lysoPC), synergizes with a membrane-permeable diacylglycerol, 1,2-dioctanoylglycerol, and ionomycin to activate resting T-lymphocytes as measured by interleukin-2 alpha-receptor expression. Phosphatidylcholines 33-52 phospholipase A2 group IB Homo sapiens 77-93 2059651-1 1991 We investigate various models of the hydrolysis of gel-phase phosphatidylcholine monolayers by phospholipase A2 (Grainger et al. Phosphatidylcholines 61-80 phospholipase A2 group IB Homo sapiens 95-111 1828227-6 1991 A dose-dependent inhibition of phospholipase A2 by both annexins VI and IV, at millimolar concentrations of Ca2+ was observed when phosphatidylcholine liposomes were used as a substrate. Phosphatidylcholines 131-150 phospholipase A2 group IB Homo sapiens 31-47 2007582-3 1991 In the absence of added Ca2+ adriamycin caused a 3-4-fold activation of hydrolysis of this pyrenelipid whereas an inhibition of action of PLA2 on the corresponding phosphatidylcholine derivative C28-O-PHPC was observed. Phosphatidylcholines 164-183 phospholipase A2 group IB Homo sapiens 138-142 2027252-2 1991 PLA2 activity was measured by radiochemical method using phosphatidylcholine as a substrate. Phosphatidylcholines 57-76 phospholipase A2 group IB Homo sapiens 0-4 2223864-2 1990 The hydrolysis of phosphatidylcholine in vitro catalyzed by porcine pancreatic PLA2 was inhibited by heparin. Phosphatidylcholines 18-37 phospholipase A2 group IB Homo sapiens 79-83 2322573-4 1990 HDL subfractions were then treated with or without phospholipase A2 from Crotalus adamanteus in presence of albumin leading to a 72-82% phosphatidylcholine degradation. Phosphatidylcholines 136-155 phospholipase A2 group IB Homo sapiens 51-67 2340304-6 1990 Results with human platelet cytosol were highly suggestive for the presence of an arachidonoyl-selective phospholipase A2 when separate phosphatidylcholine species were assayed. Phosphatidylcholines 136-155 phospholipase A2 group IB Homo sapiens 105-121 1697768-8 1990 The interaction between the mAbs and the hydrophobic moieties of PC molecules was further studied by analyzing the effect of the mAbs on the activities of phospholipase A2 and phospholipase C. JE-1 inhibited both enzyme activities, while JE-8 inhibited only the phospholipase C activity, indicating that JE-1 interacts more thoroughly with the hydrophobic region of the PC molecule than JE-8 does. Phosphatidylcholines 65-67 phospholipase A2 group IB Homo sapiens 155-171 2386385-6 1990 There were, however, lower concentrations of phosphatidylcholine and phosphatidylcholine palmitate content in infants colonised by organisms with reported phospholipase A2 activity. Phosphatidylcholines 45-64 phospholipase A2 group IB Homo sapiens 155-171 2305210-3 1990 The influence of bile salts was thus similar to that which has earlier been described for the hydrolysis of phosphatidylcholine (PC) with pig pancreatic phospholipase A2. Phosphatidylcholines 108-127 phospholipase A2 group IB Homo sapiens 153-169 2138608-1 1990 Studies are reported on the inhibition of phospholipase A2 (PLA2) from porcine pancreas, cobra (Naja naja) venom, and the P388D1 macrophage-like cell line by human recombinant lipocortin I and bovine lung calpactin I. Membrane vesicles prepared from 1-stearoyl,2-arachidonoyl phosphatidylcholine (PC) and other PCs were utilized as substrate. Phosphatidylcholines 297-299 phospholipase A2 group IB Homo sapiens 42-58 2138608-1 1990 Studies are reported on the inhibition of phospholipase A2 (PLA2) from porcine pancreas, cobra (Naja naja) venom, and the P388D1 macrophage-like cell line by human recombinant lipocortin I and bovine lung calpactin I. Membrane vesicles prepared from 1-stearoyl,2-arachidonoyl phosphatidylcholine (PC) and other PCs were utilized as substrate. Phosphatidylcholines 297-299 phospholipase A2 group IB Homo sapiens 60-64 1968928-4 1990 This release is largely independent of PLC activation and is mediated by a PLA2 because: 1) phosphatidylcholine is the preferential source of [3H]arachidonate release; 2) [3H]arachidonic acid release and phosphatidylcholine hydrolysis are blocked by two inhibitors of solubilized PLA2, mepacrine, and 4-p-bromophenacylbromide; and 3) we evidenced a PLA2 activity in cell homogenates. Phosphatidylcholines 92-111 phospholipase A2 group IB Homo sapiens 75-79 1968928-4 1990 This release is largely independent of PLC activation and is mediated by a PLA2 because: 1) phosphatidylcholine is the preferential source of [3H]arachidonate release; 2) [3H]arachidonic acid release and phosphatidylcholine hydrolysis are blocked by two inhibitors of solubilized PLA2, mepacrine, and 4-p-bromophenacylbromide; and 3) we evidenced a PLA2 activity in cell homogenates. Phosphatidylcholines 92-111 phospholipase A2 group IB Homo sapiens 280-284 1968928-4 1990 This release is largely independent of PLC activation and is mediated by a PLA2 because: 1) phosphatidylcholine is the preferential source of [3H]arachidonate release; 2) [3H]arachidonic acid release and phosphatidylcholine hydrolysis are blocked by two inhibitors of solubilized PLA2, mepacrine, and 4-p-bromophenacylbromide; and 3) we evidenced a PLA2 activity in cell homogenates. Phosphatidylcholines 92-111 phospholipase A2 group IB Homo sapiens 280-284 1968928-4 1990 This release is largely independent of PLC activation and is mediated by a PLA2 because: 1) phosphatidylcholine is the preferential source of [3H]arachidonate release; 2) [3H]arachidonic acid release and phosphatidylcholine hydrolysis are blocked by two inhibitors of solubilized PLA2, mepacrine, and 4-p-bromophenacylbromide; and 3) we evidenced a PLA2 activity in cell homogenates. Phosphatidylcholines 204-223 phospholipase A2 group IB Homo sapiens 75-79 2111711-6 1990 Based upon these results, we also conclude that the combined hydrolysis of phosphatidylcholine and phosphatidylinositol by phospholipase A2 serves as a major source for eicosanoid biosynthesis in thrombin-stimulated human platelets. Phosphatidylcholines 75-94 phospholipase A2 group IB Homo sapiens 123-139 2305210-3 1990 The influence of bile salts was thus similar to that which has earlier been described for the hydrolysis of phosphatidylcholine (PC) with pig pancreatic phospholipase A2. Phosphatidylcholines 129-131 phospholipase A2 group IB Homo sapiens 153-169 2742867-0 1989 18O isotope exchange experiments on phospholipase A2 determined by 13C-NMR: monomeric phosphatidylcholine and micellar phosphatidylethanolamine substrates. Phosphatidylcholines 86-105 phospholipase A2 group IB Homo sapiens 36-52 2402761-7 1990 The melittin-stimulated PLA2 activity observed in cells was primarily associated with phosphatidylcholine. Phosphatidylcholines 86-105 phospholipase A2 group IB Homo sapiens 24-28 35497501-4 2022 found that the fluorescently tagged C2 domain of phospholipase A2 binds to membrane phosphatidylcholine and thus labels vesicle membrane, allowing for super-resolution and electron microscopic visualization of vesicle trafficking. Phosphatidylcholines 84-103 phospholipase A2 group IB Homo sapiens 49-65 2513324-6 1989 Inhibitors of protein kinase C prevented the stimulated recycling of phosphatidylcholine, and the simultaneous induction of platelet-activating factor synthesis, by inhibiting phospholipase A2 activation. Phosphatidylcholines 69-88 phospholipase A2 group IB Homo sapiens 176-192 2774696-1 1989 The hydrolysis of radiolabelled Escherichia coli phospholipids, and micellar dispersions of phosphatidylethanolamine and phosphatidylcholine, were used to characterise the phospholipase A2 activity in synovial fluid from patients with rheumatoid arthritis. Phosphatidylcholines 121-140 phospholipase A2 group IB Homo sapiens 172-188 2758074-1 1989 The specificity of snake venom phospholipase A2(PLA2) towards a number of phospholipid (PL) substrates, e. g., phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) organized in Triton X-100 mixed micelles, liposomes and proteoliposomes was studied. Phosphatidylcholines 111-130 phospholipase A2 group IB Homo sapiens 31-47 2705543-10 1989 The acetylhydrolase activity was different from phospholipase A2, since phosphatidylcholine was not a substrate for the enzyme. Phosphatidylcholines 72-91 phospholipase A2 group IB Homo sapiens 48-64 2758074-1 1989 The specificity of snake venom phospholipase A2(PLA2) towards a number of phospholipid (PL) substrates, e. g., phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) organized in Triton X-100 mixed micelles, liposomes and proteoliposomes was studied. Phosphatidylcholines 132-134 phospholipase A2 group IB Homo sapiens 31-47 3403556-0 1988 Hydrolysis of phosphatidylcholine in phosphatidylcholine-cholate mixtures by porcine pancreatic phospholipase A2. Phosphatidylcholines 14-33 phospholipase A2 group IB Homo sapiens 96-112 2523541-6 1989 Whereas photooxidized egg phosphatidylcholine liposomes underwent total LOOH loss when incubated with PLA2 and GSH/Gpx, no net loss was observed with photooxidized cholesterol/dimyristoyl-phosphatidylcholine liposomes. Phosphatidylcholines 26-45 phospholipase A2 group IB Homo sapiens 102-106 2498670-2 1989 Both substances can be generated intracellularly by the action of phospholipase A2 on phosphatidylcholine. Phosphatidylcholines 86-105 phospholipase A2 group IB Homo sapiens 66-82 3149278-3 1988 Phosphatidylcholine or phosphatidylethanolamine with arachidonate at the sn-2 position of glycerol was cleaved efficiently by phospholipase A2 activity in homogenates as well as in the cytoplasmic fraction of human platelets, leading to the selective liberation of free arachidonate, whereas phospholipids with linoleate were hardly hydrolyzed under the same conditions. Phosphatidylcholines 0-19 phospholipase A2 group IB Homo sapiens 126-142 3235916-0 1988 Cyclopentanoid analogs of phosphatidylcholine: susceptibility to phospholipase A2. Phosphatidylcholines 26-45 phospholipase A2 group IB Homo sapiens 65-81 2927050-1 1989 A position-specifically labelled phosphatidylcholine is the substrate for the selective determination of Phospholipase A2 in serum, ascites and tissue samples. Phosphatidylcholines 33-52 phospholipase A2 group IB Homo sapiens 105-121 2500989-4 1989 Phospholipase A2 is activated by a variety of physical and chemical agents (e.g. infection, trauma) that increase calcium concentrations in the cell; it releases arachidonic acid from phosphatidyl-choline and phosphatidyl-ethanolamine in particular. Phosphatidylcholines 184-204 phospholipase A2 group IB Homo sapiens 0-16 3264481-2 1988 It was postulated that phospholipase A2 (PLA2) exposure would potentiate porcine pancreatic elastase (PPE)-induced epithelial solute permeability in a manner similar to that which was previously shown with lysophosphatidylcholine (lysoPC), a naturally occurring, membrane-perturbing agent that is formed principally through the hydrolysis of phosphatidylcholine by PLA2. Phosphatidylcholines 210-229 phospholipase A2 group IB Homo sapiens 41-45 3403556-0 1988 Hydrolysis of phosphatidylcholine in phosphatidylcholine-cholate mixtures by porcine pancreatic phospholipase A2. Phosphatidylcholines 37-56 phospholipase A2 group IB Homo sapiens 96-112 3403556-1 1988 Pancreatic phospholipase A2 (PLA2)-catalyzed hydrolysis of egg yolk phosphatidylcholine (PC) in mixed PC-cholate systems depends upon composition, structure, and size of the mixed aggregates. Phosphatidylcholines 68-87 phospholipase A2 group IB Homo sapiens 11-27 3403556-1 1988 Pancreatic phospholipase A2 (PLA2)-catalyzed hydrolysis of egg yolk phosphatidylcholine (PC) in mixed PC-cholate systems depends upon composition, structure, and size of the mixed aggregates. Phosphatidylcholines 68-87 phospholipase A2 group IB Homo sapiens 29-33 3403556-1 1988 Pancreatic phospholipase A2 (PLA2)-catalyzed hydrolysis of egg yolk phosphatidylcholine (PC) in mixed PC-cholate systems depends upon composition, structure, and size of the mixed aggregates. Phosphatidylcholines 89-91 phospholipase A2 group IB Homo sapiens 11-27 3403556-1 1988 Pancreatic phospholipase A2 (PLA2)-catalyzed hydrolysis of egg yolk phosphatidylcholine (PC) in mixed PC-cholate systems depends upon composition, structure, and size of the mixed aggregates. Phosphatidylcholines 89-91 phospholipase A2 group IB Homo sapiens 29-33 3134504-5 1988 Arachidonic acid utilized in the production of eicosanoids is derived from phospholipids by the action of phospholipase A2 and phospholipase C. When U937 cells were cultured in medium supplemented with gamma-interferon, there was a striking increase in the level of phosphatidylcholine and phosphatidylethanolamine-specific phospholipase A2 activities and phosphatidylinositol-specific phospholipase C activity as compared to control cells. Phosphatidylcholines 266-285 phospholipase A2 group IB Homo sapiens 106-122 3238311-6 1988 Moreover, the phospholipase A2 inhibitor, nordihydroguaiaretic acid significantly inhibited PC degradation, lysoPC formation, and NAG release, whereas the lipoxygenase inhibitor, BW 755C had little effect on these parameters. Phosphatidylcholines 92-94 phospholipase A2 group IB Homo sapiens 14-30 3198053-6 1988 The PA2 activity associated with normal spermatozoa exhibited a 60% decrease in activity after storage at -20 degrees C for 48 hr followed by a heating period of 10 min at 60 degrees C. Long-term storage of spermatozoa at -20 degrees C also resulted in a similar decrease in the deacylation of PC. Phosphatidylcholines 294-296 phospholipase A2 group IB Homo sapiens 4-7 3129527-5 1988 Therefore, phospholipase A2 activity was calculated as the rate of the release of arachidonic acid from phosphatidylcholine under the conditions used. Phosphatidylcholines 104-123 phospholipase A2 group IB Homo sapiens 11-27 2958081-5 1987 It was also found that the degradation of liposomal phosphatidylcholine by soluble snake venom PLA2 is inversely proportional to the solvent viscosity. Phosphatidylcholines 52-71 phospholipase A2 group IB Homo sapiens 95-99 2447937-3 1987 On the other hand, gramicidin enhances the rate of cleavage of outer membrane layer phosphatidylcholine by phospholipase A2, which indicates changes in the packing of phosphatidylcholine following gramicidin binding. Phosphatidylcholines 84-103 phospholipase A2 group IB Homo sapiens 107-123 2447937-3 1987 On the other hand, gramicidin enhances the rate of cleavage of outer membrane layer phosphatidylcholine by phospholipase A2, which indicates changes in the packing of phosphatidylcholine following gramicidin binding. Phosphatidylcholines 167-186 phospholipase A2 group IB Homo sapiens 107-123 3631062-2 1987 PLase A2-induced conversion of phosphatidyl choline (PC) to lysophosphatidyl choline (L-PC) was associated with a marked increase in Na+ influx and Ca2+ uptake. Phosphatidylcholines 31-51 phospholipase A2 group IB Homo sapiens 0-8 3631062-2 1987 PLase A2-induced conversion of phosphatidyl choline (PC) to lysophosphatidyl choline (L-PC) was associated with a marked increase in Na+ influx and Ca2+ uptake. Phosphatidylcholines 53-55 phospholipase A2 group IB Homo sapiens 0-8 3123482-2 1988 We have studied the phospholipase A2 activity in fractionated human neutrophils, employing labeled phosphatidylinositol, phosphatidylcholine, and phosphatidylethanolamine as exogenous substrates. Phosphatidylcholines 121-140 phospholipase A2 group IB Homo sapiens 20-36 3123482-10 1988 Phosphatidylinositol was a better substrate for the plasma membrane enzyme, whereas phosphatidylcholine and phosphatidylethanolamine behaved as better substrates for intracellular organelle phospholipase A2 activities. Phosphatidylcholines 84-103 phospholipase A2 group IB Homo sapiens 190-206 3625042-0 1987 Synthesis of a naphthylvinyl-labeled glycerol ether analog of phosphatidylcholine and its use in the assay of phospholipase A2. Phosphatidylcholines 62-81 phospholipase A2 group IB Homo sapiens 110-126 3566802-0 1987 Influence of cationic amphiphilic drugs on the phosphatidylcholine hydrolysis by phospholipase A2. Phosphatidylcholines 47-66 phospholipase A2 group IB Homo sapiens 81-97 3112131-4 1987 The loss of [3H]arachidonate radioactivity from phosphatidylcholine was almost equivalent to the increase in released [3H]arachidonic acid, suggesting the hydrolysis of phosphatidylcholine by phospholipase A2. Phosphatidylcholines 169-188 phospholipase A2 group IB Homo sapiens 192-208 3549734-3 1987 This stimulatory protein was stable for several months when frozen at -70 degrees C. The purified protein selectively stimulated phospholipase A2 when phosphatidylcholine was used as a substrate but had no effect on phospholipase A2 activity when phosphatidylethanolamine was used as a substrate. Phosphatidylcholines 151-170 phospholipase A2 group IB Homo sapiens 129-145 3559272-4 1987 The activities of both phospholipase A2 and C were assayed in each sample using phosphatidylcholine and phosphatidylinositol, respectively, as substrates. Phosphatidylcholines 80-99 phospholipase A2 group IB Homo sapiens 23-39 2434471-3 1986 Purified PLA2 had absolute 2-acyl specificity, and hydrolyzed phosphatidylcholine with optimal activity at pH 7.5-8.0 and phosphatidylethanolamine with optimal activity at pH 7.0. Phosphatidylcholines 62-81 phospholipase A2 group IB Homo sapiens 9-13 3593300-3 1987 Membrane PL organization was detected by Bee venom phospholipase-A2 (Plase) treatment, which specifically hydrolyzes outer bilayer phosphatidylserine (PS), phosphatidylethanolamine (PE), and phosphatidylcholine (PC). Phosphatidylcholines 191-210 phospholipase A2 group IB Homo sapiens 51-67 3593300-3 1987 Membrane PL organization was detected by Bee venom phospholipase-A2 (Plase) treatment, which specifically hydrolyzes outer bilayer phosphatidylserine (PS), phosphatidylethanolamine (PE), and phosphatidylcholine (PC). Phosphatidylcholines 212-214 phospholipase A2 group IB Homo sapiens 51-67 3730428-0 1986 Glucose transport into human erythrocytes treated with phospholipase A2 or C. Phospholipase A2 induced crenation of human erythrocytes and decreased glucose transport activity (influx rate) by 40% when 51% of phosphatidylcholine (PC) in the membrane was hydrolyzed. Phosphatidylcholines 209-228 phospholipase A2 group IB Homo sapiens 55-71 3730428-0 1986 Glucose transport into human erythrocytes treated with phospholipase A2 or C. Phospholipase A2 induced crenation of human erythrocytes and decreased glucose transport activity (influx rate) by 40% when 51% of phosphatidylcholine (PC) in the membrane was hydrolyzed. Phosphatidylcholines 209-228 phospholipase A2 group IB Homo sapiens 78-94 3730428-0 1986 Glucose transport into human erythrocytes treated with phospholipase A2 or C. Phospholipase A2 induced crenation of human erythrocytes and decreased glucose transport activity (influx rate) by 40% when 51% of phosphatidylcholine (PC) in the membrane was hydrolyzed. Phosphatidylcholines 230-232 phospholipase A2 group IB Homo sapiens 55-71 3730428-0 1986 Glucose transport into human erythrocytes treated with phospholipase A2 or C. Phospholipase A2 induced crenation of human erythrocytes and decreased glucose transport activity (influx rate) by 40% when 51% of phosphatidylcholine (PC) in the membrane was hydrolyzed. Phosphatidylcholines 230-232 phospholipase A2 group IB Homo sapiens 78-94 3004591-5 1986 Treatment of the phosphatidylcholine fraction with phospholipase A2 released 64% of the 5-[3H]HETE with 26% remaining in the lysophosphatidylcholine fraction. Phosphatidylcholines 17-36 phospholipase A2 group IB Homo sapiens 51-67 3964311-4 1986 Enhanced PLA2 activities were observed in RA patient cells when phosphatidylcholine (PC) or phosphatidylethanolamine (PE) were used as substrates. Phosphatidylcholines 64-83 phospholipase A2 group IB Homo sapiens 9-13 3964311-4 1986 Enhanced PLA2 activities were observed in RA patient cells when phosphatidylcholine (PC) or phosphatidylethanolamine (PE) were used as substrates. Phosphatidylcholines 85-87 phospholipase A2 group IB Homo sapiens 9-13 3775794-0 1986 Hydrolysis of short-chain phosphatidylcholines by bee venom phospholipase A2. Phosphatidylcholines 26-46 phospholipase A2 group IB Homo sapiens 60-76 3948674-0 1986 [The role of cytochrome P-450 in the activation of phosphatidyl choline hydrolysis by phospholipase A2]. Phosphatidylcholines 51-71 phospholipase A2 group IB Homo sapiens 86-102 4016572-1 1985 Addition of cardiolipin or diacylglycerol to dispersions of phosphatidylcholine greatly increased hydrolysis by snake venom or pancreatic phospholipase A2, as well as by a microbial phospholipase. Phosphatidylcholines 60-79 phospholipase A2 group IB Homo sapiens 138-154 4056060-5 1985 Finally, an increase in cell recognition as determined by monocyte phagocytosis and adherence in vitro, as well as decreased phosphatidylcholine accessibility to bee venom phospholipase A2, was found in H2O2-treated erythrocytes compared with controls. Phosphatidylcholines 125-144 phospholipase A2 group IB Homo sapiens 172-188 3862730-4 1985 Peripheral blood PMN from patients with RA (RA-PMN) exhibit greater phospholipase A2 activities against phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and greater phospholipase C activities against PC, PE, and phosphatidylinositol (PI) than PMN obtained from normal volunteers (N-PMN). Phosphatidylcholines 104-123 phospholipase A2 group IB Homo sapiens 68-84 3862730-4 1985 Peripheral blood PMN from patients with RA (RA-PMN) exhibit greater phospholipase A2 activities against phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and greater phospholipase C activities against PC, PE, and phosphatidylinositol (PI) than PMN obtained from normal volunteers (N-PMN). Phosphatidylcholines 125-127 phospholipase A2 group IB Homo sapiens 68-84 3841007-1 1985 The effect of the altered polar head group of phosphatidylcholine (PC) on its transbilayer distributions in small unilamellar vesicles containing sphingomyelin (SM) was ascertained with phospholipase A2 as the external membrane probe. Phosphatidylcholines 46-65 phospholipase A2 group IB Homo sapiens 186-202 3841007-1 1985 The effect of the altered polar head group of phosphatidylcholine (PC) on its transbilayer distributions in small unilamellar vesicles containing sphingomyelin (SM) was ascertained with phospholipase A2 as the external membrane probe. Phosphatidylcholines 67-69 phospholipase A2 group IB Homo sapiens 186-202 4052390-6 1985 Cobra venom phospholipase A2 hydrolyzes monomeric short-chain PE"s at about the same rate as short-chain PC"s but hydrolyzes long-chain PC"s much more rapidly than long-chain PE"s. Phosphatidylcholines 105-107 phospholipase A2 group IB Homo sapiens 12-28 4052390-6 1985 Cobra venom phospholipase A2 hydrolyzes monomeric short-chain PE"s at about the same rate as short-chain PC"s but hydrolyzes long-chain PC"s much more rapidly than long-chain PE"s. Phosphatidylcholines 136-138 phospholipase A2 group IB Homo sapiens 12-28 4001746-1 1985 Phosphatidyl ethanolamine methylase (PEMT) is an enzyme involved in the methylation of membrane phospholipids which plays a very important role in the modulation of the activity of the beta-receptors and the production of phosphatidylcholine, substrate of phospholipase A2. Phosphatidylcholines 222-241 phospholipase A2 group IB Homo sapiens 256-272 3926598-4 1985 When phosphatidylcholine micelles were incubated with the enzyme in the presence of gabexate mesilate, the mode of inhibition of the enzyme action by the drug appeared to be noncompetitive and Ki of gabexate mesilate for phospholipase A2 was 0.77 mM. Phosphatidylcholines 5-24 phospholipase A2 group IB Homo sapiens 221-237 4092816-1 1985 The hydrolysis of acyl esters in phosphatidylcholine by phospholipase A2 (PLA2) for human placental blood vessel was investigated. Phosphatidylcholines 33-52 phospholipase A2 group IB Homo sapiens 56-72 4092816-1 1985 The hydrolysis of acyl esters in phosphatidylcholine by phospholipase A2 (PLA2) for human placental blood vessel was investigated. Phosphatidylcholines 33-52 phospholipase A2 group IB Homo sapiens 74-78 4092816-3 1985 In contrast to rat tissues, the human placental blood vessel PLA2 showed a selective preference for arachidonate over linoleate acyl group at the sn-2 position of phosphatidylcholine. Phosphatidylcholines 163-182 phospholipase A2 group IB Homo sapiens 61-65 6517928-4 1984 31P-NMR of phosphatidylcholine indicates that diacylglycerol causes an isotropic component to develop in the spectrum of the bilayers which correlates approximately with the enhancement of phospholipase A2 attack. Phosphatidylcholines 11-30 phospholipase A2 group IB Homo sapiens 189-205 6441309-7 1984 These results suggest that endogenous phospholipase A2 may break membrane phosphatidyl choline into lysophosphatidyl choline and fatty acid, when the acrosome reaction occurs. Phosphatidylcholines 74-94 phospholipase A2 group IB Homo sapiens 38-54 6644446-5 1983 Phosphatidylcholine and phosphatidylethanolamine were digested by phospholipase A2. Phosphatidylcholines 0-19 phospholipase A2 group IB Homo sapiens 66-82 6468604-2 1984 Manoalide was also found to inactivate purified phospholipase A2 and thus prevent hydrolysis of phosphatidylcholine. Phosphatidylcholines 96-115 phospholipase A2 group IB Homo sapiens 48-64 6746652-7 1984 These results indicate that the 1-acyl-2-arachidonoyl species of phosphatidylcholine are not exclusively degraded by phospholipase A2 activity in thrombin-stimulated platelets and suggest that the differential compartmentation of molecular species of phosphatidylcholine according to their metabolic origins can influence their apparent susceptibility to hydrolysis. Phosphatidylcholines 65-84 phospholipase A2 group IB Homo sapiens 117-133 6871217-1 1983 By making use of the capacity of phospholipase A2 to degrade selectively the phospholipid in the outer half of the lipid bilayer of small unilamellar phospholipid/cholesterol vesicles without affecting the retention of a vesicle-encapsulated solute, we demonstrated that the exchange of phosphatidylcholine between such vesicles and human high density lipoprotein involves exclusively the phosphatidylcholine present in the outer monolayer of the vesicle membrane. Phosphatidylcholines 287-306 phospholipase A2 group IB Homo sapiens 33-49 6411702-2 1983 All the phosphatidylcholine molecules in those particles were readily digested by phospholipase A2 while only the molecules in the outer leaflet of phosphatidylcholine unilamellar vesicles were hydrolyzed under the same conditions. Phosphatidylcholines 8-27 phospholipase A2 group IB Homo sapiens 82-98 6871217-1 1983 By making use of the capacity of phospholipase A2 to degrade selectively the phospholipid in the outer half of the lipid bilayer of small unilamellar phospholipid/cholesterol vesicles without affecting the retention of a vesicle-encapsulated solute, we demonstrated that the exchange of phosphatidylcholine between such vesicles and human high density lipoprotein involves exclusively the phosphatidylcholine present in the outer monolayer of the vesicle membrane. Phosphatidylcholines 389-408 phospholipase A2 group IB Homo sapiens 33-49 6807835-3 1982 Phospholipase A2 digestion of the intact erythrocytes resulted in a diminution of exclusively phosphatidylcholine (PC) from the membrane, and an approximately parallel loss of Rh antigen activity at 37 degrees to about 50% of the original. Phosphatidylcholines 94-113 phospholipase A2 group IB Homo sapiens 0-16 6863295-7 1983 At various time intervals, samples were taken and treated with phospholipase A2, which selectively degrades the PC in the outer monolayer. Phosphatidylcholines 112-114 phospholipase A2 group IB Homo sapiens 63-79 6852018-0 1983 Hydrolysis of mixed monomolecular films of phosphatidylcholine/triacylglycerol by pancreatic phospholipase A2. Phosphatidylcholines 43-62 phospholipase A2 group IB Homo sapiens 93-109 6838196-0 1983 Pancreatic porcine phospholipase A2 catalyzed hydrolysis of phosphatidylcholine in lecithin-bile salt mixed micelles: kinetic studies in a lecithin-sodium cholate system. Phosphatidylcholines 60-79 phospholipase A2 group IB Homo sapiens 19-35 6838196-1 1983 Pancreatic porcine phospholipase A2 catalyzed hydrolysis of phosphatidylcholine in bile salt lecithin mixed micelles has been studied, utilizing a series of assay mixtures for which the micellar size, weight, and composition had been experimentally determined. Phosphatidylcholines 60-79 phospholipase A2 group IB Homo sapiens 19-35 6831908-2 1983 Bee venom phospholipase A2 which attacks only phosphatidylcholine (PC) in the intact erythrocyte results in inhibition of cryohemolysis produced by both hypertonic sodium chloride and sucrose. Phosphatidylcholines 46-65 phospholipase A2 group IB Homo sapiens 10-26 6831908-2 1983 Bee venom phospholipase A2 which attacks only phosphatidylcholine (PC) in the intact erythrocyte results in inhibition of cryohemolysis produced by both hypertonic sodium chloride and sucrose. Phosphatidylcholines 67-69 phospholipase A2 group IB Homo sapiens 10-26 7139847-2 1982 Furthermore, it was found that at the phase transition temperature the phosphatidylcholine bilayers are subject to rapid hydrolysis by pancreatic phospholipase A2 whereas phosphatidylethanolamine bilayers are not. Phosphatidylcholines 71-90 phospholipase A2 group IB Homo sapiens 146-162 6807835-3 1982 Phospholipase A2 digestion of the intact erythrocytes resulted in a diminution of exclusively phosphatidylcholine (PC) from the membrane, and an approximately parallel loss of Rh antigen activity at 37 degrees to about 50% of the original. Phosphatidylcholines 115-117 phospholipase A2 group IB Homo sapiens 0-16 7306536-1 1981 Previous studies have revealed that the replacement of the C-2 ester group in phosphatidylcholine by the carbamyloxy function renders the resulting lipids, without affecting the properties of the liposomes, resistant to hydrolysis by phospholipase A2 (Gupta, C.M. Phosphatidylcholines 78-97 phospholipase A2 group IB Homo sapiens 234-250 7195282-0 1980 Pancreatic phospholipase A2 hydrolysis of phosphatidylcholines in various physicochemical states. Phosphatidylcholines 42-62 phospholipase A2 group IB Homo sapiens 11-27 7311737-0 1981 Metabolism of epoxidized phosphatidylcholine by phospholipase A2 and epoxide hydrolase. Phosphatidylcholines 25-44 phospholipase A2 group IB Homo sapiens 48-64 6943585-8 1981 Peripheral blood mononuclear cells were also directly shown to display phospholipase A2-like activity, as measured by the decrease in radioactive arachidonate from prelabeled phospholipids, specifically phosphatidylcholine, in effector cells. Phosphatidylcholines 203-222 phospholipase A2 group IB Homo sapiens 71-87 7462211-6 1981 The concomitant increases in lysophosphatidylcholine and decreases in phosphatidylcholine, as well as the decreases in phosphatidylethanolamine, can only be explained by the stimulation of phospholipase A2 activity in platelets by thrombin. Phosphatidylcholines 33-52 phospholipase A2 group IB Homo sapiens 189-205 6273927-2 1981 PC formed by transmethylation is further metabolized by phospholipase A2. Phosphatidylcholines 0-2 phospholipase A2 group IB Homo sapiens 56-72 7195282-7 1980 During an extended incubation, however, nearly all intralipid phosphatidylcholines and only half the bile phosphatidylcholines were hydrolyzed by pancreatic phospholipase A2. Phosphatidylcholines 62-82 phospholipase A2 group IB Homo sapiens 157-173 677904-0 1978 Effect of the state of phosphatidylcholine on the rate of its hydrolysis by phospholipase A2 (bee venom). Phosphatidylcholines 23-42 phospholipase A2 group IB Homo sapiens 76-92 27520426-0 1978 Rapid enzyme-induced hydrolysis of microgram amounts of phosphatidylcholine on phospholipase A2/celite columns. Phosphatidylcholines 56-75 phospholipase A2 group IB Homo sapiens 79-95 7214196-4 1980 It is concluded that LCAT possesses highly specific steric and positional requirements for its phosphatidylcholine substrates and that, like phospholipase A2, it has a significant activity with the 2-sn-phosphorylcholinedicacylglycerol. Phosphatidylcholines 95-114 phospholipase A2 group IB Homo sapiens 141-157 7411590-0 1980 Action of phospholipase A2 on unmodified phosphatidylcholine bilayers: organizational defects are preferred sites of action. Phosphatidylcholines 41-60 phospholipase A2 group IB Homo sapiens 10-26 7411590-1 1980 The hydrolytic action of the bee venom phospholipase A2 on phosphatidylcholine bilayers is studied under a variety of conditions that introduce alterations in the packing, such as those induced by sonication, gel to liquid crystalline phase transition, and osmotic shock. Phosphatidylcholines 59-78 phospholipase A2 group IB Homo sapiens 39-55 582173-1 1979 The activity of phospholipase A2 from cobra venom toward phospholipid in single-walled, sonicated vesicles was analyzed, particularly with respect to its activity toward the saturated phosphatidylcholines in the gel and liquid crystalline states. Phosphatidylcholines 184-204 phospholipase A2 group IB Homo sapiens 16-32 454594-6 1979 From the initial velocity rate, the time required for the phosphatidylcholine pool to double was about 12 h. Agarose-linked phospholipase A2 was used to measure the relative composition of choline- and dimethylethanolamine-phosphoglycerides in the outer surface of vesicles prepared from cells with different degrees of polar head group substitution. Phosphatidylcholines 58-77 phospholipase A2 group IB Homo sapiens 124-140 459721-4 1979 Three times more elaidic than oleic acid (OI) accumulated in the 1-acyl position of phosphatidylcholine, as determined by hydrolysis with phospholipase A2. Phosphatidylcholines 84-103 phospholipase A2 group IB Homo sapiens 138-154 565217-2 1978 We examined the action of porcine pancreatic and bee-venom phospholipase A2 towards bilayers of phosphatidylcholine as a function of several physical characteristics of the lipid-water interface. Phosphatidylcholines 96-115 phospholipase A2 group IB Homo sapiens 59-75 565217-17 1978 Vesicles composed of egg phosphatidylcholine can be degraded by pancreatic phospholipase A2 at 37 degrees C, provided that the substrate bilayer is strongly curved. Phosphatidylcholines 25-44 phospholipase A2 group IB Homo sapiens 75-91 849774-0 1977 [Action of chlorphentermine on the hydrolysis of phosphatidyl choline by phospholipase A2 (author"s transl)]. Phosphatidylcholines 49-69 phospholipase A2 group IB Homo sapiens 73-89 952985-12 1976 As in seminal plasma phospholipase A2, the activity in crude Naja naja venom towards sonicated radioactively labelled phosphatidylcholine was stimulated at low and inhibited at high concentrations of dibucaine and chloropromazine, for example. Phosphatidylcholines 118-137 phospholipase A2 group IB Homo sapiens 21-37 1174523-1 1975 The phospholipases A2, C and D have been used to investigate the localization of phosphatidylcholine in the phosphatidylcholine exchange protein from beef liver. Phosphatidylcholines 81-100 phospholipase A2 group IB Homo sapiens 4-30 1276220-4 1976 Pancreatic phospholipase A2, an enzyme unable to hydrolyse the phospholipids of intact erythrocytes, partially degrades phosphatidylcholine and phosphatidylethanolamine of erythrocytes pretreated with hexanol or SH reagents. Phosphatidylcholines 120-139 phospholipase A2 group IB Homo sapiens 11-27 1174523-1 1975 The phospholipases A2, C and D have been used to investigate the localization of phosphatidylcholine in the phosphatidylcholine exchange protein from beef liver. Phosphatidylcholines 108-127 phospholipase A2 group IB Homo sapiens 4-30 173287-1 1975 Hydrolysis of phosphatidylcholine by phospholipase A2 of synaptic membranes i n Tris-CHl buffer was stimulated by cyclic AMP, cyclic GMP, cyclic CMP, cyclic UMP and adenosine (0.1 mm). Phosphatidylcholines 14-33 phospholipase A2 group IB Homo sapiens 37-53 30830399-8 2018 Phosphatidylcholines, lyso-phosphatidylcholines and fatty acids were significantly changed among pathological samples suggesting changes in phospholipase A2 and arachidonic acid metabolic pathways. Phosphatidylcholines 0-20 phospholipase A2 group IB Homo sapiens 140-156 31273804-1 2019 Lysophosphatidylcholine (lysoPtdCho) is produced mainly by the phospholipase A2-dependent hydrolysis of phosphatidylcholine (PtdCho) and can induce inflammatory activation and osteogenic gene expression in vascular smooth muscle cells. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 63-79 31273804-1 2019 Lysophosphatidylcholine (lysoPtdCho) is produced mainly by the phospholipase A2-dependent hydrolysis of phosphatidylcholine (PtdCho) and can induce inflammatory activation and osteogenic gene expression in vascular smooth muscle cells. Phosphatidylcholines 29-35 phospholipase A2 group IB Homo sapiens 63-79 31788037-3 2019 To examine the substrate utilization of PE and PC by PLA2, we developed a method to accurately detect and measure specific forms of PE and PC as low as 50 femtomoles. Phosphatidylcholines 47-49 phospholipase A2 group IB Homo sapiens 53-57 31788037-3 2019 To examine the substrate utilization of PE and PC by PLA2, we developed a method to accurately detect and measure specific forms of PE and PC as low as 50 femtomoles. Phosphatidylcholines 139-141 phospholipase A2 group IB Homo sapiens 53-57 31788037-4 2019 Validation of this method consisted of an enzymatic assay to monitor docosahexaenoic acid and arachidonic acid release from the hydrolysis of PE and PC by group IV phospholipase A2 (cPLA2alpha) coupled to the generation of lyso-PE (LPE) and lyso-PC (LPC). Phosphatidylcholines 149-151 phospholipase A2 group IB Homo sapiens 164-180 31788037-4 2019 Validation of this method consisted of an enzymatic assay to monitor docosahexaenoic acid and arachidonic acid release from the hydrolysis of PE and PC by group IV phospholipase A2 (cPLA2alpha) coupled to the generation of lyso-PE (LPE) and lyso-PC (LPC). Phosphatidylcholines 246-248 phospholipase A2 group IB Homo sapiens 164-180 31788037-7 2019 This new UPLC ESI-MS/MS method provides accurate and highly sensitive detection of PE and PC species containing AA and DHA allowing for the specific examination of the substrate utilization of these phospholipids by PLA2 in vitro and in cells. Phosphatidylcholines 90-92 phospholipase A2 group IB Homo sapiens 216-220 30845751-5 2019 LPC is mainly derived from the turnover of phosphatidylcholine (PC) in the circulation by phospholipase A2 (PLA2). Phosphatidylcholines 43-62 phospholipase A2 group IB Homo sapiens 90-106 30845751-5 2019 LPC is mainly derived from the turnover of phosphatidylcholine (PC) in the circulation by phospholipase A2 (PLA2). Phosphatidylcholines 43-62 phospholipase A2 group IB Homo sapiens 108-112 30845751-5 2019 LPC is mainly derived from the turnover of phosphatidylcholine (PC) in the circulation by phospholipase A2 (PLA2). Phosphatidylcholines 1-3 phospholipase A2 group IB Homo sapiens 90-106 30845751-5 2019 LPC is mainly derived from the turnover of phosphatidylcholine (PC) in the circulation by phospholipase A2 (PLA2). Phosphatidylcholines 1-3 phospholipase A2 group IB Homo sapiens 108-112 4474005-0 1974 The activity of phospholipase A2 in reversed micelles of phosphatidylcholine in diethyl ether: effect of water and cations. Phosphatidylcholines 57-76 phospholipase A2 group IB Homo sapiens 16-32 4407745-0 1974 Hydrolysis of phosphatidylcholine liposomes by pancreatic phospholipase A2 at the transition temperature. Phosphatidylcholines 14-33 phospholipase A2 group IB Homo sapiens 58-74 4798723-0 1973 Phospholipase A2 activity towards phosphatidylcholine in mixed micelles: surface dilution kinetics and the effect of thermotropic phase transitions. Phosphatidylcholines 34-53 phospholipase A2 group IB Homo sapiens 0-16 32491269-3 2020 Results demonstrated that hypogonadism was associated with a significant increase in sphingomyelin (SM), whereas phosphatidylcholine (PC) was mainly cleaved by activated phospholipase-A2 into lysophosphatidylcholine (LPC). Phosphatidylcholines 113-132 phospholipase A2 group IB Homo sapiens 170-186 32491269-3 2020 Results demonstrated that hypogonadism was associated with a significant increase in sphingomyelin (SM), whereas phosphatidylcholine (PC) was mainly cleaved by activated phospholipase-A2 into lysophosphatidylcholine (LPC). Phosphatidylcholines 134-136 phospholipase A2 group IB Homo sapiens 170-186 32084434-2 2020 LPC originates from the cleavage of phosphatidylcholine by phospholipase A2 (PLA2). Phosphatidylcholines 36-55 phospholipase A2 group IB Homo sapiens 59-75 32084434-2 2020 LPC originates from the cleavage of phosphatidylcholine by phospholipase A2 (PLA2). Phosphatidylcholines 36-55 phospholipase A2 group IB Homo sapiens 77-81 28864658-4 2018 Here, we propose that 2 enzymes-phospholipase A2 (PLA2) and ectonucleotide pyrophophatase/phosphodiesterase 6 (ENPP6)-act in sequence upon phosphatidylcholine found in MV membranes to produce phosphocholine, which PHOSPHO1 can hydrolyze to liberate Pi This hypothesis is supported by evidence that both enzymes are expressed in mineralizing cells and data showing that phosphatidylcholine is broken down in MVs during mineralization. Phosphatidylcholines 139-158 phospholipase A2 group IB Homo sapiens 32-48 29132829-0 2018 The lipolytic degradation of highly structured cubic micellar nanoparticles of soy phosphatidylcholine and glycerol dioleate by phospholipase A2 and triacylglycerol lipase. Phosphatidylcholines 83-102 phospholipase A2 group IB Homo sapiens 128-144 28864658-4 2018 Here, we propose that 2 enzymes-phospholipase A2 (PLA2) and ectonucleotide pyrophophatase/phosphodiesterase 6 (ENPP6)-act in sequence upon phosphatidylcholine found in MV membranes to produce phosphocholine, which PHOSPHO1 can hydrolyze to liberate Pi This hypothesis is supported by evidence that both enzymes are expressed in mineralizing cells and data showing that phosphatidylcholine is broken down in MVs during mineralization. Phosphatidylcholines 369-388 phospholipase A2 group IB Homo sapiens 32-48 28864658-4 2018 Here, we propose that 2 enzymes-phospholipase A2 (PLA2) and ectonucleotide pyrophophatase/phosphodiesterase 6 (ENPP6)-act in sequence upon phosphatidylcholine found in MV membranes to produce phosphocholine, which PHOSPHO1 can hydrolyze to liberate Pi This hypothesis is supported by evidence that both enzymes are expressed in mineralizing cells and data showing that phosphatidylcholine is broken down in MVs during mineralization. Phosphatidylcholines 369-388 phospholipase A2 group IB Homo sapiens 50-54 28864658-4 2018 Here, we propose that 2 enzymes-phospholipase A2 (PLA2) and ectonucleotide pyrophophatase/phosphodiesterase 6 (ENPP6)-act in sequence upon phosphatidylcholine found in MV membranes to produce phosphocholine, which PHOSPHO1 can hydrolyze to liberate Pi This hypothesis is supported by evidence that both enzymes are expressed in mineralizing cells and data showing that phosphatidylcholine is broken down in MVs during mineralization. Phosphatidylcholines 139-158 phospholipase A2 group IB Homo sapiens 50-54 30689326-9 2018 The anti-tuberculosis chemotherapy gives rise to activation of phospholipase A2 that shows membrane destructive effect on mononuclears of peripheral blood and results in disorganization of their membranes manifesting in cumulation of lysophospholipids at simultaneous decreasing of percentage content of phosphatidylserine and phosphatidylcholine. Phosphatidylcholines 327-346 phospholipase A2 group IB Homo sapiens 63-79 29773019-2 2017 PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA). Phosphatidylcholines 221-240 phospholipase A2 group IB Homo sapiens 0-4 24404395-1 2014 PURPOSE: Asthma is a chronic inflammatory disease of the airways, and is associated with upregulation of phospholipase A2 (PLA2), the enzyme that hydrolyzes phosphatidylcholine, producing lysophosphatidylcholine (LPC) and free fatty acids. Phosphatidylcholines 157-176 phospholipase A2 group IB Homo sapiens 105-121 26721598-6 2016 We will show that oxidative modifications of PL by HOCl have a considerable impact on the PLA2 digestibility, i.e., oxidation of the unsaturated fatty acyl residues leads to a reduced digestibility of both PC and PE. Phosphatidylcholines 206-208 phospholipase A2 group IB Homo sapiens 90-94 23894007-1 2014 BACKGROUND: Lysophosphatidylcholine (LPC), a derivative of phosphatidylcholine (PC) hydrolyzed by phospholipase A2 (PLA2), is reported to be increased in bile of the patients with pancreaticobiliary maljunction or intrahepatic cholelithiasis, both of which are major risk factors for biliary tract cancers with undefined etiology. Phosphatidylcholines 16-35 phospholipase A2 group IB Homo sapiens 98-114 23894007-1 2014 BACKGROUND: Lysophosphatidylcholine (LPC), a derivative of phosphatidylcholine (PC) hydrolyzed by phospholipase A2 (PLA2), is reported to be increased in bile of the patients with pancreaticobiliary maljunction or intrahepatic cholelithiasis, both of which are major risk factors for biliary tract cancers with undefined etiology. Phosphatidylcholines 16-35 phospholipase A2 group IB Homo sapiens 116-120 23894007-1 2014 BACKGROUND: Lysophosphatidylcholine (LPC), a derivative of phosphatidylcholine (PC) hydrolyzed by phospholipase A2 (PLA2), is reported to be increased in bile of the patients with pancreaticobiliary maljunction or intrahepatic cholelithiasis, both of which are major risk factors for biliary tract cancers with undefined etiology. Phosphatidylcholines 38-40 phospholipase A2 group IB Homo sapiens 98-114 23894007-1 2014 BACKGROUND: Lysophosphatidylcholine (LPC), a derivative of phosphatidylcholine (PC) hydrolyzed by phospholipase A2 (PLA2), is reported to be increased in bile of the patients with pancreaticobiliary maljunction or intrahepatic cholelithiasis, both of which are major risk factors for biliary tract cancers with undefined etiology. Phosphatidylcholines 38-40 phospholipase A2 group IB Homo sapiens 116-120 23894007-7 2014 CONCLUSIONS: These data suggest that PLA2, which catalyzes the hydrolysis of PC to yield LPC and free fatty acid, is supposed to be an important etiological factor in BECs injury in pancreaticobiliary maljunction or intrahepatic cholelithiasis. Phosphatidylcholines 77-79 phospholipase A2 group IB Homo sapiens 37-41 25490414-1 2014 Phospholipase A2 (PLA2) changes the phosphatidylcholine contained in low-density lipoprotein (LDL) to lysophosphatidylcholine (LPC), which has various proatherogenic properties. Phosphatidylcholines 36-55 phospholipase A2 group IB Homo sapiens 0-16 25490414-1 2014 Phospholipase A2 (PLA2) changes the phosphatidylcholine contained in low-density lipoprotein (LDL) to lysophosphatidylcholine (LPC), which has various proatherogenic properties. Phosphatidylcholines 36-55 phospholipase A2 group IB Homo sapiens 18-22 24404395-1 2014 PURPOSE: Asthma is a chronic inflammatory disease of the airways, and is associated with upregulation of phospholipase A2 (PLA2), the enzyme that hydrolyzes phosphatidylcholine, producing lysophosphatidylcholine (LPC) and free fatty acids. Phosphatidylcholines 157-176 phospholipase A2 group IB Homo sapiens 123-127 24404396-3 2014 Furthermore, pathological lung conditions are associated with upregulated phospholipase A2 (PLA2), the predominant enzyme producing LPC in tissues by hydrolysis of phosphatidylcholine. Phosphatidylcholines 164-183 phospholipase A2 group IB Homo sapiens 74-90 24404396-3 2014 Furthermore, pathological lung conditions are associated with upregulated phospholipase A2 (PLA2), the predominant enzyme producing LPC in tissues by hydrolysis of phosphatidylcholine. Phosphatidylcholines 164-183 phospholipase A2 group IB Homo sapiens 92-96 24251714-7 2013 Lysophosphatidylcholine (lysoPC), a derivative of phosphatidylcholine hydrolysis by phospholipase A2, is a highly abundant bioactive lipid mediator present in circulation as well as in bile. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 84-100 21511545-2 2011 Phospholipase A(2) (PLA(2)) quantitation in real-time, using (7-nitro-2-1,3-benzoxadiazol-4-yl)amino-derivatives of phosphatidylcholine (NBD-PCs) as substrates, is influenced by high protein content, color or turbidity. Phosphatidylcholines 116-135 phospholipase A2 group IB Homo sapiens 0-18 23850486-2 2013 Here, we showed that liposomes consisting of phosphatidylserine and lysophosphatidylcholine, a lipolysis product of phosphatidylcholine by PLA2, were phagocytosed by microglia, but failed to induce secretion of PGE2. Phosphatidylcholines 72-91 phospholipase A2 group IB Homo sapiens 139-143 23727005-0 2013 Synthesis of mixed-chain phosphatidylcholines including coumarin fluorophores for FRET-based kinetic studies of phospholipase A(2) enzymes. Phosphatidylcholines 25-45 phospholipase A2 group IB Homo sapiens 112-130 23727005-4 2013 Here we report a new synthesis of double-labeled phosphatidylcholine analogs with chain-terminal reporter groups including coumarin fluorophores for fluorescence resonance energy transfer (FRET)-based kinetic studies of PLA2 enzymes. Phosphatidylcholines 49-68 phospholipase A2 group IB Homo sapiens 220-224 23727005-8 2013 Specifically, the rate of PLA2 hydrolysis of the coumarin labeled phosphatidylcholine analogs was less than three times slower than the natural substrate dipalmitoyl phosphatidylcholine (DPPC) under the same experimental conditions. Phosphatidylcholines 66-85 phospholipase A2 group IB Homo sapiens 26-30 23604781-2 2013 A model for delivering dietary sn-2-DHA phosphatidylcholine (PtdCho) to the brain involves phospholipase A2 based deacylation/reacylation cycle followed by delivery of DHA through high-density lipoproteins that bind to the brain capillary endothelial cells in the blood-brain barrier (BBB). Phosphatidylcholines 61-67 phospholipase A2 group IB Homo sapiens 91-107 23604781-6 2013 The data presented here show that: (1) group X secretory PLA2 (sPLA2) is about threefold more active than group V sPLA2 in releasing sn-2 fatty acids from DHA regioisomers, and (2) EL shows its specificity for DHA PtdCho species in a concentration independent manner, suggesting that the enzyme could play a major role in generating free sn-1-DHA or/and sn-2-DHA lysoPtdCho from the regioisomers in the BBB. Phosphatidylcholines 214-220 phospholipase A2 group IB Homo sapiens 57-61 21575006-2 2011 LysoPCs are thought to be derived from cell membrane degradation products such as phosphatidylcholines (PC) by partial hydrolysis of PC, a process that is catalyzed by phospholipase A(2) (PLA(2) ). Phosphatidylcholines 82-102 phospholipase A2 group IB Homo sapiens 168-186 21575006-2 2011 LysoPCs are thought to be derived from cell membrane degradation products such as phosphatidylcholines (PC) by partial hydrolysis of PC, a process that is catalyzed by phospholipase A(2) (PLA(2) ). Phosphatidylcholines 82-102 phospholipase A2 group IB Homo sapiens 189-195 21575006-2 2011 LysoPCs are thought to be derived from cell membrane degradation products such as phosphatidylcholines (PC) by partial hydrolysis of PC, a process that is catalyzed by phospholipase A(2) (PLA(2) ). Phosphatidylcholines 4-6 phospholipase A2 group IB Homo sapiens 168-186 21575006-2 2011 LysoPCs are thought to be derived from cell membrane degradation products such as phosphatidylcholines (PC) by partial hydrolysis of PC, a process that is catalyzed by phospholipase A(2) (PLA(2) ). Phosphatidylcholines 4-6 phospholipase A2 group IB Homo sapiens 189-195 21575006-2 2011 LysoPCs are thought to be derived from cell membrane degradation products such as phosphatidylcholines (PC) by partial hydrolysis of PC, a process that is catalyzed by phospholipase A(2) (PLA(2) ). Phosphatidylcholines 104-106 phospholipase A2 group IB Homo sapiens 168-186 21575006-2 2011 LysoPCs are thought to be derived from cell membrane degradation products such as phosphatidylcholines (PC) by partial hydrolysis of PC, a process that is catalyzed by phospholipase A(2) (PLA(2) ). Phosphatidylcholines 104-106 phospholipase A2 group IB Homo sapiens 189-195 23505262-0 2013 Retraction: CDP-choline significantly restores phosphatidylcholine levels by differentially affecting phospholipase A2 and CTP: phosphocholine cytidylyltransferase after stroke. Phosphatidylcholines 47-66 phospholipase A2 group IB Homo sapiens 102-163 21511545-2 2011 Phospholipase A(2) (PLA(2)) quantitation in real-time, using (7-nitro-2-1,3-benzoxadiazol-4-yl)amino-derivatives of phosphatidylcholine (NBD-PCs) as substrates, is influenced by high protein content, color or turbidity. Phosphatidylcholines 116-135 phospholipase A2 group IB Homo sapiens 20-26 18084901-10 2007 The phospholipase A2 activity plays an important role in lung surfactant homeostasis and is responsible for the bulk of the degradation of internalized phosphatidylcholine and its resynthesis by the reacylation pathway. Phosphatidylcholines 152-171 phospholipase A2 group IB Homo sapiens 4-20 21094990-3 2011 PLA(2) activity in TEU-2 cells was measured using (16:0, [(3)H]18:1) plasmenylcholine and phosphatidylcholine substrates in the presence and absence of calcium. Phosphatidylcholines 90-109 phospholipase A2 group IB Homo sapiens 0-6 19837049-0 2010 Synthesis of oligo(ethylene glycol) substituted phosphatidylcholines: secretory PLA2-targeted precursors of NSAID prodrugs. Phosphatidylcholines 48-68 phospholipase A2 group IB Homo sapiens 80-84 19184454-6 2009 This last result was obtained through the study of structured phosphatidylcholine selective deacylation using a phospholipase A2. Phosphatidylcholines 62-81 phospholipase A2 group IB Homo sapiens 112-128 17311918-1 2007 Snake presynaptic phospholipase A2 neurotoxins (SPANs) bind to the presynaptic membrane and hydrolyze phosphatidylcholine with generation of lysophosphatidylcholine (LysoPC) and fatty acid (FA). Phosphatidylcholines 102-121 phospholipase A2 group IB Homo sapiens 18-34 19615464-4 2009 The proteins were active with various phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs), and for most of substrates the PLA(1) activity was much higher than the PLA(2) activity. Phosphatidylcholines 38-58 phospholipase A2 group IB Homo sapiens 174-180 19615464-4 2009 The proteins were active with various phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs), and for most of substrates the PLA(1) activity was much higher than the PLA(2) activity. Phosphatidylcholines 60-63 phospholipase A2 group IB Homo sapiens 174-180 19102680-4 2009 Analysis of the data using a kinetic model has suggested an allosteric mechanism for the rate increase of hPLA2 by cholate and also for the rate-lowering effect by certain bile salts or cembranoids on the cholate-activated hPLA2 hydrolysis of phosphatidylcholine vesicles. Phosphatidylcholines 243-262 phospholipase A2 group IB Homo sapiens 106-111 19102680-4 2009 Analysis of the data using a kinetic model has suggested an allosteric mechanism for the rate increase of hPLA2 by cholate and also for the rate-lowering effect by certain bile salts or cembranoids on the cholate-activated hPLA2 hydrolysis of phosphatidylcholine vesicles. Phosphatidylcholines 243-262 phospholipase A2 group IB Homo sapiens 223-228 17169434-0 2007 A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH. Phosphatidylcholines 67-86 phospholipase A2 group IB Homo sapiens 35-51 16380371-0 2006 CDP-choline significantly restores phosphatidylcholine levels by differentially affecting phospholipase A2 and CTP: phosphocholine cytidylyltransferase after stroke. Phosphatidylcholines 35-54 phospholipase A2 group IB Homo sapiens 90-106 16754327-2 2006 The PC and other glycerophospholipid compositions of membranes change dynamically through stimulus-dependent and independent pathways, principally by the action of two different types of enzymes; phospholipase A2 [EC 3.1.1.4] and acyl-CoA:lysophospholipid acyltransferase [EC 2.3.1.23]. Phosphatidylcholines 4-6 phospholipase A2 group IB Homo sapiens 196-212 16380371-3 2006 PtdCho is hydrolyzed by phospholipase A2 (PLA2), PtdChospecific phospholipase C (PtdCho-PLC), and phospholipase D (PLD). Phosphatidylcholines 0-6 phospholipase A2 group IB Homo sapiens 24-40 16380371-3 2006 PtdCho is hydrolyzed by phospholipase A2 (PLA2), PtdChospecific phospholipase C (PtdCho-PLC), and phospholipase D (PLD). Phosphatidylcholines 0-6 phospholipase A2 group IB Homo sapiens 42-46 15220446-2 2004 We have identified a phospholipase A2 (PLA2) activity borne by HCMV by using an assay based on the hydrolysis of fluorescent phosphatidylcholine. Phosphatidylcholines 125-144 phospholipase A2 group IB Homo sapiens 21-37 15756928-5 2005 CDP-choline might increase the PC levels by attenuating PLA(2) stimulation and loss of CCT activity. Phosphatidylcholines 31-33 phospholipase A2 group IB Homo sapiens 56-62 15175345-3 2004 In the present paper, we optimized two independent assays for the translocation of natural phosphatidylcholine (PC) to the cell surface based on the hydrolysis of outer leaflet phosphoglycerolipids by exogenous phospholipase A2 and the exchange of outer leaflet PC by a transfer protein. Phosphatidylcholines 91-110 phospholipase A2 group IB Homo sapiens 211-227 15175345-3 2004 In the present paper, we optimized two independent assays for the translocation of natural phosphatidylcholine (PC) to the cell surface based on the hydrolysis of outer leaflet phosphoglycerolipids by exogenous phospholipase A2 and the exchange of outer leaflet PC by a transfer protein. Phosphatidylcholines 112-114 phospholipase A2 group IB Homo sapiens 211-227 16213900-1 2005 BACKGROUND: Lysophosphatidylcholine (LysoPC) is a product of phosphatidylcholine hydrolysis by phospholipase A2, which is associated with atherosclerosis. Phosphatidylcholines 16-35 phospholipase A2 group IB Homo sapiens 95-111 15220446-2 2004 We have identified a phospholipase A2 (PLA2) activity borne by HCMV by using an assay based on the hydrolysis of fluorescent phosphatidylcholine. Phosphatidylcholines 125-144 phospholipase A2 group IB Homo sapiens 39-43 15231944-3 2004 After the first case, we hypothesized that abnormally high BA levels could have reversed the action of phospholipase A2 in the lungs, causing a degradation of phosphatidylcholines to lysophosphatidylcholines and the consequent lack of surfactant activity, leading to the severe respiratory distress. Phosphatidylcholines 159-179 phospholipase A2 group IB Homo sapiens 103-119 15202762-1 2004 Lysophosphatidylcholine (lyso-PTC) is formed by phospholipase A2 (PLA2) from phosphatidylcholine (PTC), that is produced through phosphatidylethanolamine (PTE) methylation. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 48-64 15202762-1 2004 Lysophosphatidylcholine (lyso-PTC) is formed by phospholipase A2 (PLA2) from phosphatidylcholine (PTC), that is produced through phosphatidylethanolamine (PTE) methylation. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 66-70 15202762-1 2004 Lysophosphatidylcholine (lyso-PTC) is formed by phospholipase A2 (PLA2) from phosphatidylcholine (PTC), that is produced through phosphatidylethanolamine (PTE) methylation. Phosphatidylcholines 30-33 phospholipase A2 group IB Homo sapiens 48-64 15202762-1 2004 Lysophosphatidylcholine (lyso-PTC) is formed by phospholipase A2 (PLA2) from phosphatidylcholine (PTC), that is produced through phosphatidylethanolamine (PTE) methylation. Phosphatidylcholines 30-33 phospholipase A2 group IB Homo sapiens 66-70 15455713-0 2004 [Bromoacyl analogues of phosphatidylcholine with intramolecular fluorescence quenching and their use as substrates for continuous monitoring of phospholipase A2 activity]. Phosphatidylcholines 24-43 phospholipase A2 group IB Homo sapiens 144-160 15079868-3 2004 Phospholipase A(2) (PLA(2)) hydrolyzes PtdCho to produce free fatty acids and lyso-PtdCho, an inhibitor of CCT. Phosphatidylcholines 39-45 phospholipase A2 group IB Homo sapiens 0-18 15079868-3 2004 Phospholipase A(2) (PLA(2)) hydrolyzes PtdCho to produce free fatty acids and lyso-PtdCho, an inhibitor of CCT. Phosphatidylcholines 39-45 phospholipase A2 group IB Homo sapiens 20-26 12271462-2 2002 PtdCho hydrolysis by phospholipase A(2) (PLA(2)) after cerebral ischemia and reperfusion yields arachidonic acid (ArAc) and lyso-PtdCho. Phosphatidylcholines 0-6 phospholipase A2 group IB Homo sapiens 21-39 15844632-1 2004 Lysophosphatidylcholine (lysoPC) is generated by the action of phospholipase A2 on membrane phosphatidylcholine, the most abundant cellular phospholipid. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 63-79 12940517-9 2003 In order to get more reliable results, we recommend that clinicians and researchers use NBD-phosphatidylcholines as PLA2 substrates in biological samples and start with an analytical separation of reaction products followed by image analysis of the fluorescent spots. Phosphatidylcholines 92-112 phospholipase A2 group IB Homo sapiens 116-120 12764097-5 2003 Exposure of cortical neurons to neurotoxic concentrations of NMDA increased extracellular choline and activated hydrolysis of phosphatidylcholine and phosphatidylinositol by phospholipase A2 but did not induce significant degradation of phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, or phosphatidylserine. Phosphatidylcholines 126-145 phospholipase A2 group IB Homo sapiens 174-190 12472619-4 2002 As a model system, liposomes composed of phosphatidylcholines (PC) from egg yolk were digested by phospholipase A2 (PLA2). Phosphatidylcholines 41-61 phospholipase A2 group IB Homo sapiens 98-114 12472619-4 2002 As a model system, liposomes composed of phosphatidylcholines (PC) from egg yolk were digested by phospholipase A2 (PLA2). Phosphatidylcholines 41-61 phospholipase A2 group IB Homo sapiens 116-120 12472619-4 2002 As a model system, liposomes composed of phosphatidylcholines (PC) from egg yolk were digested by phospholipase A2 (PLA2). Phosphatidylcholines 63-65 phospholipase A2 group IB Homo sapiens 98-114 12472619-4 2002 As a model system, liposomes composed of phosphatidylcholines (PC) from egg yolk were digested by phospholipase A2 (PLA2). Phosphatidylcholines 63-65 phospholipase A2 group IB Homo sapiens 116-120 12271462-2 2002 PtdCho hydrolysis by phospholipase A(2) (PLA(2)) after cerebral ischemia and reperfusion yields arachidonic acid (ArAc) and lyso-PtdCho. Phosphatidylcholines 0-6 phospholipase A2 group IB Homo sapiens 41-47 11694619-5 2001 Uptake of micellar beta-carotene and lutein was greatly suppressed by phosphatidylcholine (PC) in a dose-dependent manner, whereas lysophosphatidylcholine (lysoPC), the lipolysis product of PC by phospholipase A2 (PLA2), markedly enhanced both beta-carotene and lutein uptake. Phosphatidylcholines 91-93 phospholipase A2 group IB Homo sapiens 196-212 11694619-6 2001 The addition of PLA2 from porcine pancreas to the medium also enhanced the uptake of carotenoids from micelles containing PC. Phosphatidylcholines 122-124 phospholipase A2 group IB Homo sapiens 16-20 12471472-2 2002 We recently reported that PLA(2) mediates the hydrolysis of phosphatidylcholine (PC) to lysophosphatidylcholine (L-PC) when both are applied to the apical surface of cultures enterocyte monolayers, resulting in increased bacterial translocation (BT) and decreased transepithelial electrical resistance (TEER). Phosphatidylcholines 60-79 phospholipase A2 group IB Homo sapiens 26-32 12471472-2 2002 We recently reported that PLA(2) mediates the hydrolysis of phosphatidylcholine (PC) to lysophosphatidylcholine (L-PC) when both are applied to the apical surface of cultures enterocyte monolayers, resulting in increased bacterial translocation (BT) and decreased transepithelial electrical resistance (TEER). Phosphatidylcholines 81-83 phospholipase A2 group IB Homo sapiens 26-32 11694619-5 2001 Uptake of micellar beta-carotene and lutein was greatly suppressed by phosphatidylcholine (PC) in a dose-dependent manner, whereas lysophosphatidylcholine (lysoPC), the lipolysis product of PC by phospholipase A2 (PLA2), markedly enhanced both beta-carotene and lutein uptake. Phosphatidylcholines 91-93 phospholipase A2 group IB Homo sapiens 214-218 11409160-2 2001 We recently reported that PLA2 mediates hydrolysis of phosphatidylcholine (PC) to lysophosphatidylcholine (L-PC) when both are applied to the apical surface of cultured EC monolayers, resulting in increased bacterial translocation (BT) and decreased transepithelial electrical resistance (TEER). Phosphatidylcholines 54-73 phospholipase A2 group IB Homo sapiens 26-30 11409160-2 2001 We recently reported that PLA2 mediates hydrolysis of phosphatidylcholine (PC) to lysophosphatidylcholine (L-PC) when both are applied to the apical surface of cultured EC monolayers, resulting in increased bacterial translocation (BT) and decreased transepithelial electrical resistance (TEER). Phosphatidylcholines 75-77 phospholipase A2 group IB Homo sapiens 26-30 11409160-5 2001 PLA2 may also mediate the hydrolysis of luminal phospholipids other than PC. Phosphatidylcholines 73-75 phospholipase A2 group IB Homo sapiens 0-4 10888232-5 2000 Since phosphatidylcholine is metabolized mainly by the action of phospholipase A2, with the release of arachidonic acid and other fatty acids, the effect of phosphocholine on arachidonic acid release in endothelial cells was also examined. Phosphatidylcholines 6-25 phospholipase A2 group IB Homo sapiens 65-81 10972869-8 2000 Recombinant NtPat1 and NtPat3 enzymes were active in an assay using labelled bacterial membranes, and also displayed high bona fide PLA2 activity on phosphatidylcholine substrate. Phosphatidylcholines 149-168 phospholipase A2 group IB Homo sapiens 132-136 11080676-3 2000 gVPLA(2) is homologous to other group II PLA(2) family members but has distinctive enzymatic properties, including its activity to effectively hydrolyze phosphatidylcholine (PC) vesicles and the outer plasma membrane of mammalian cells. Phosphatidylcholines 153-172 phospholipase A2 group IB Homo sapiens 2-8 11129952-6 2000 The mechanism for the reduction in radiolabelled fatty acid incorporation into phosphatidylcholine was a 64% (p < 0.05) reduction in membrane phospholipase A2 activity. Phosphatidylcholines 79-98 phospholipase A2 group IB Homo sapiens 145-161 10717249-9 2000 These are phosphatidylinositol-4, 5-bisphosphate-specific phospholipase C and phosphatidylethanolamine-specific phospholipase D. A positive feedback mechanism for DG production which includes these phospholipases, a phosphatidylcholine-specific phospholipase C and a phosphatidylcholine-specific phospholipase A2 has also been suggested. Phosphatidylcholines 216-235 phospholipase A2 group IB Homo sapiens 296-312 10692455-2 2000 The photosystem II core dimers were treated with phospholipase A2 (PL-A2), which cuts phosphatidylglycerol (PG) and phosphatidylcholine molecules at the sn-2 position. Phosphatidylcholines 116-135 phospholipase A2 group IB Homo sapiens 49-65 10692455-2 2000 The photosystem II core dimers were treated with phospholipase A2 (PL-A2), which cuts phosphatidylglycerol (PG) and phosphatidylcholine molecules at the sn-2 position. Phosphatidylcholines 116-135 phospholipase A2 group IB Homo sapiens 67-72 10196168-7 1999 Similarly, treatment of acetyl LDL with phospholipase A2 converted more than 90% of the initial content of phosphatidylcholine (PC) to lyso-PC, but the phospholipase A2-treated acetyl LDL was nearly 10-fold less potent than oxidized LDL at stimulating growth. Phosphatidylcholines 128-130 phospholipase A2 group IB Homo sapiens 40-56 10196168-7 1999 Similarly, treatment of acetyl LDL with phospholipase A2 converted more than 90% of the initial content of phosphatidylcholine (PC) to lyso-PC, but the phospholipase A2-treated acetyl LDL was nearly 10-fold less potent than oxidized LDL at stimulating growth. Phosphatidylcholines 107-126 phospholipase A2 group IB Homo sapiens 40-56 9724516-8 1998 Studies on the influence of quinacrine on the activity of PLA2 toward pyrene-labeled phospholipid analogues revealed that the hydrolysis of phosphatidylcholine was progressively reduced as a function of increasing [quinacrine]. Phosphatidylcholines 140-159 phospholipase A2 group IB Homo sapiens 58-62 10191282-1 1999 In vitro hydrolysis of human lipoprotein[a] (Lp[a]) by phospholipase A2 (PLA2) decreased the phosphatidylcholine (PC) content by 85%, but increased nonesterified fatty acids 3.2-fold and lysoPC 12.9-fold. Phosphatidylcholines 93-112 phospholipase A2 group IB Homo sapiens 55-71 10191282-1 1999 In vitro hydrolysis of human lipoprotein[a] (Lp[a]) by phospholipase A2 (PLA2) decreased the phosphatidylcholine (PC) content by 85%, but increased nonesterified fatty acids 3.2-fold and lysoPC 12.9-fold. Phosphatidylcholines 93-112 phospholipase A2 group IB Homo sapiens 73-77 10191282-1 1999 In vitro hydrolysis of human lipoprotein[a] (Lp[a]) by phospholipase A2 (PLA2) decreased the phosphatidylcholine (PC) content by 85%, but increased nonesterified fatty acids 3.2-fold and lysoPC 12.9-fold. Phosphatidylcholines 114-116 phospholipase A2 group IB Homo sapiens 55-71 10191282-1 1999 In vitro hydrolysis of human lipoprotein[a] (Lp[a]) by phospholipase A2 (PLA2) decreased the phosphatidylcholine (PC) content by 85%, but increased nonesterified fatty acids 3.2-fold and lysoPC 12.9-fold. Phosphatidylcholines 114-116 phospholipase A2 group IB Homo sapiens 73-77 10217630-3 1999 The reciprocal nature of the decrease in phosphatidylcholine concentration, compared with the increase in the concentration of 1-lysophosphatidylcholine and 2-lysophosphatidylcholine, suggested a substrate/product relationship consistent with the activities of phospholipase A1 and phospholipase A2, respectively. Phosphatidylcholines 41-60 phospholipase A2 group IB Homo sapiens 282-298 9786921-3 1998 Sphingomyelinase (SMase) and phospholipase A2 (PLA2) have been found in the arterial wall, and, moreover, lesional LDL shows signs of hydrolysis of both sphingomyelin and phosphatidylcholine. Phosphatidylcholines 171-190 phospholipase A2 group IB Homo sapiens 29-45 9786921-3 1998 Sphingomyelinase (SMase) and phospholipase A2 (PLA2) have been found in the arterial wall, and, moreover, lesional LDL shows signs of hydrolysis of both sphingomyelin and phosphatidylcholine. Phosphatidylcholines 171-190 phospholipase A2 group IB Homo sapiens 47-51 10192509-3 1999 LPC is formed by hydrolysis of phosphatidylcholine (PC) in LDL and cell membranes, induced by phospholipase A2 or by oxidation. Phosphatidylcholines 31-50 phospholipase A2 group IB Homo sapiens 94-110 10192509-3 1999 LPC is formed by hydrolysis of phosphatidylcholine (PC) in LDL and cell membranes, induced by phospholipase A2 or by oxidation. Phosphatidylcholines 1-3 phospholipase A2 group IB Homo sapiens 94-110 9569615-1 1998 Porcine pancreatic phospholipase A2 (PLA2) hydrolyses phosphatidylcholine when in the lamellar state as well as in the micellar state. Phosphatidylcholines 54-73 phospholipase A2 group IB Homo sapiens 19-35 9692961-1 1998 Bilayers composed of phosphatidylcholine initially resist catalysis by phospholipase A2. Phosphatidylcholines 21-40 phospholipase A2 group IB Homo sapiens 71-87 9626147-10 1998 Lyso PtdCho, a product derived from phospholipase A2 action on peroxidized phosphatidylcholine and a potent chemotactic factor for monocytes and T-lymphocytes, is elevated in endometriosis. Phosphatidylcholines 75-94 phospholipase A2 group IB Homo sapiens 36-52 9506985-1 1998 Lysophosphatidylcholine (lyso-PC) is a product of phosphatidylcholine hydrolysis by phospholipase A2 (PLA2) and is present in cell membranes, oxidized lipoproteins, and atherosclerotic tissues. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 84-100 9506985-1 1998 Lysophosphatidylcholine (lyso-PC) is a product of phosphatidylcholine hydrolysis by phospholipase A2 (PLA2) and is present in cell membranes, oxidized lipoproteins, and atherosclerotic tissues. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 102-106 9643351-0 1998 Phospholipase A2 relieves phosphatidylcholine inhibition of micellar cholesterol absorption and transport by human intestinal cell line Caco-2. Phosphatidylcholines 26-45 phospholipase A2 group IB Homo sapiens 0-16 9585574-4 1998 In the present study, Fourier transform infrared spectroscopy (FTIR) has been used to monitor the hydrolysis of phosphatidylcholine by phospholipase A2 (PLA2) from Naja mocambique mocambique venom in the presence of various aminoglycosides and/or daptomycin. Phosphatidylcholines 112-131 phospholipase A2 group IB Homo sapiens 135-151 9585574-4 1998 In the present study, Fourier transform infrared spectroscopy (FTIR) has been used to monitor the hydrolysis of phosphatidylcholine by phospholipase A2 (PLA2) from Naja mocambique mocambique venom in the presence of various aminoglycosides and/or daptomycin. Phosphatidylcholines 112-131 phospholipase A2 group IB Homo sapiens 153-157 9648220-5 1998 This Amadori product formation was also confirmed in PE/phosphate buffer dispersions and in phosphatidylcholine-PE liposome/phosphate buffer suspensions in the presence of D-glucose at 37 degrees C. gPE was degraded by phospholipase A2, C and D. Phosphatidylcholines 92-111 phospholipase A2 group IB Homo sapiens 219-244 9531469-4 1998 First, hV-PLA2 can catalyse the hydrolysis of phosphatidylcholine more effectively than hIIa-PLA2 by two orders of magnitude. Phosphatidylcholines 46-65 phospholipase A2 group IB Homo sapiens 10-14 9531469-5 1998 Secondly, hV-PLA2 has much higher binding affinity and activity for compactly packed phosphatidylcholine bilayers than hIIa-PLA2. Phosphatidylcholines 85-104 phospholipase A2 group IB Homo sapiens 13-17 9569615-1 1998 Porcine pancreatic phospholipase A2 (PLA2) hydrolyses phosphatidylcholine when in the lamellar state as well as in the micellar state. Phosphatidylcholines 54-73 phospholipase A2 group IB Homo sapiens 37-41 9526092-2 1998 LPC is produced as a result of PC hydrolysis by several isoforms of phospholipase A2 (PLA2) and in the reaction mediated by lecithin-cholesterol acyltransferase that transfers the fatty acid residue from PC to cholesterol. Phosphatidylcholines 1-3 phospholipase A2 group IB Homo sapiens 68-84 9526092-2 1998 LPC is produced as a result of PC hydrolysis by several isoforms of phospholipase A2 (PLA2) and in the reaction mediated by lecithin-cholesterol acyltransferase that transfers the fatty acid residue from PC to cholesterol. Phosphatidylcholines 1-3 phospholipase A2 group IB Homo sapiens 86-90 9428661-0 1997 Characterization and optimization of phospholipase A2 catalyzed synthesis of phosphatidylcholine. Phosphatidylcholines 77-96 phospholipase A2 group IB Homo sapiens 37-53 9428661-1 1997 The phospholipase A2 (PLA2) catalyzed synthesis and hydrolysis of phosphatidylcholine (PC) was studied in a water activity controlled organic medium. Phosphatidylcholines 66-85 phospholipase A2 group IB Homo sapiens 4-20 9428661-1 1997 The phospholipase A2 (PLA2) catalyzed synthesis and hydrolysis of phosphatidylcholine (PC) was studied in a water activity controlled organic medium. Phosphatidylcholines 66-85 phospholipase A2 group IB Homo sapiens 22-26 9428661-1 1997 The phospholipase A2 (PLA2) catalyzed synthesis and hydrolysis of phosphatidylcholine (PC) was studied in a water activity controlled organic medium. Phosphatidylcholines 87-89 phospholipase A2 group IB Homo sapiens 4-20 9428661-1 1997 The phospholipase A2 (PLA2) catalyzed synthesis and hydrolysis of phosphatidylcholine (PC) was studied in a water activity controlled organic medium. Phosphatidylcholines 87-89 phospholipase A2 group IB Homo sapiens 22-26 9266766-6 1997 An ensuing generation of lysoPC and arachidonic acid, which paralleled the occurrence of exocytosis, revealed that the newly synthesized PC was hydrolyzed by phospholipase A2. Phosphatidylcholines 29-31 phospholipase A2 group IB Homo sapiens 158-174 9153219-1 1997 Lysophosphatidylcholine (lyso-PC), a natural lipid generated through the action of phospholipase A2 on membrane phosphatidylcholine, has been implicated in atherogenesis and the inflammatory process. Phosphatidylcholines 4-23 phospholipase A2 group IB Homo sapiens 83-99 9148961-0 1997 Phosphatidylcholine hydrolysis is required for pancreatic cholesterol esterase- and phospholipase A2-facilitated cholesterol uptake into intestinal Caco-2 cells. Phosphatidylcholines 0-19 phospholipase A2 group IB Homo sapiens 84-100 9148961-8 1997 We demonstrate that both phospholipase A2 and cholesterol esterase increase cholesterol uptake by hydrolyzing the phosphatidylcholine that is used to prepare the cholesterol-containing micelles. Phosphatidylcholines 114-133 phospholipase A2 group IB Homo sapiens 25-41