PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30580923-1 2019 Sec14, the major yeast phosphatidylcholine (PC)/phosphatidylinositol (PI) transfer protein (PITP), coordinates PC and PI metabolism to facilitate an appropriate and essential lipid signaling environment for membrane trafficking from trans-Golgi membranes. Phosphatidylcholines 23-42 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 32828847-1 2021 Sec14, a yeast phosphatidylinositol/phosphatidylcholine transfer protein, functions at the trans-Golgi membranes. Phosphatidylcholines 36-55 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 30580923-1 2019 Sec14, the major yeast phosphatidylcholine (PC)/phosphatidylinositol (PI) transfer protein (PITP), coordinates PC and PI metabolism to facilitate an appropriate and essential lipid signaling environment for membrane trafficking from trans-Golgi membranes. Phosphatidylcholines 44-46 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 30580923-8 2019 Finally, wild-type Sec14, but not a mixture of Sec14 proteins specifically deficient in either PC- or PI-binding activity, was able to effect a net transfer of PI or PC down opposing concentration gradients in vitro. Phosphatidylcholines 95-97 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 19-24 27193303-0 2016 Two-ligand priming mechanism for potentiated phosphoinositide synthesis is an evolutionarily conserved feature of Sec14-like phosphatidylinositol and phosphatidylcholine exchange proteins. Phosphatidylcholines 150-169 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 114-119 27193303-4 2016 Here we demonstrate that, like the yeast Sec14, the AtSfh1 PITP domain requires both its phosphatidylinositol (PtdIns)- and phosphatidylcholine (PtdCho)-binding properties to stimulate PtdIns-4-phosphate [PtdIns(4)P] synthesis. Phosphatidylcholines 124-143 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 41-46 27193303-4 2016 Here we demonstrate that, like the yeast Sec14, the AtSfh1 PITP domain requires both its phosphatidylinositol (PtdIns)- and phosphatidylcholine (PtdCho)-binding properties to stimulate PtdIns-4-phosphate [PtdIns(4)P] synthesis. Phosphatidylcholines 145-151 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 41-46 18243114-1 2008 Sec14, the major yeast phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein, regulates essential interfaces between lipid metabolism and membrane trafficking from the trans-Golgi network (TGN). Phosphatidylcholines 53-72 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 26921357-6 2016 Herein, we describe a Sec14 motif, which we term the VV signature, that contributes significantly to the NPPM sensitivity/resistance of Sec14-like phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer proteins. Phosphatidylcholines 177-196 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 22-27 26921357-6 2016 Herein, we describe a Sec14 motif, which we term the VV signature, that contributes significantly to the NPPM sensitivity/resistance of Sec14-like phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer proteins. Phosphatidylcholines 177-196 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 136-141 26921357-6 2016 Herein, we describe a Sec14 motif, which we term the VV signature, that contributes significantly to the NPPM sensitivity/resistance of Sec14-like phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer proteins. Phosphatidylcholines 198-204 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 22-27 26921357-6 2016 Herein, we describe a Sec14 motif, which we term the VV signature, that contributes significantly to the NPPM sensitivity/resistance of Sec14-like phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer proteins. Phosphatidylcholines 198-204 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 136-141 20651827-4 2010 Sec14 was first identified as a phosphatidylcholine (PC) - phosphatidylinositol (PI) transfer protein required for viability, with reduced Sec14 function resulting in diminished vesicular transport out of the trans-Golgi. Phosphatidylcholines 32-51 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 20651827-4 2010 Sec14 was first identified as a phosphatidylcholine (PC) - phosphatidylinositol (PI) transfer protein required for viability, with reduced Sec14 function resulting in diminished vesicular transport out of the trans-Golgi. Phosphatidylcholines 53-55 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 22102037-1 2011 Sec14 is the major phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein in the yeast Saccharomyces cerevisiae and is the founding member of the Sec14 protein superfamily. Phosphatidylcholines 49-68 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 22102037-1 2011 Sec14 is the major phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein in the yeast Saccharomyces cerevisiae and is the founding member of the Sec14 protein superfamily. Phosphatidylcholines 49-68 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 167-172 19129178-1 2009 A protein known to regulate both lipid metabolism and vesicular transport is the phosphatidylcholine/phosphatidylinositol transfer protein Sec14 of Saccharomyces cerevisiae. Phosphatidylcholines 81-100 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 139-144 17601877-2 2007 Inactivation of the CDP-choline pathway for phosphatidylcholine synthesis allows cells to survive in the absence of Sec14p function through restoration of Golgi vesicular transport capability. Phosphatidylcholines 44-63 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 116-122 17325006-1 2007 Sec14p promotes the energy-independent transfer of either phosphatidylinositol (PtdIns) or phosphatidylcholine (PtdCho) between lipid bilayers in vitro and represents the major PtdIns/PtdCho transfer protein in the budding yeast Saccharomyces cerevisiae. Phosphatidylcholines 91-110 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-6 17881569-1 2007 The Saccharomyces cerevisiae phosphatidylcholine/phosphatidylinositol transfer protein Sec14p is required for Golgi apparatus-derived vesicular transport through coordinate regulation of phospholipid metabolism. Phosphatidylcholines 29-48 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 87-93 17881569-3 2007 The essential requirement for SEC14 can be bypassed by inactivation of (i) the CDP-choline pathway for phosphatidylcholine synthesis or (ii) KES1, which encodes an oxysterol binding protein. Phosphatidylcholines 103-122 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 30-35 17325006-2 2007 Herein, we employ multi-frequency high-field electron paramagnetic resonance (EPR) to analyze the electrostatic and hydrogen-bonding microenvironments for series of doxyl-labeled PtdCho molecules bound by Sec14p in a soluble protein-PtdCho complex. Phosphatidylcholines 179-185 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 205-211 17325006-6 2007 Partially resolved 130-GHz EPR spectra from n-doxyl-PtdCho bound to Sec14p were analyzed using this two-component model and allowed quantification of two parameters. Phosphatidylcholines 52-58 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 68-74 16997918-5 2006 Herein, we describe the application of EPR spectroscopy to probe the local dynamics and the electrostatic microenvironment of phosphatidylcholine (PtdCho) bound by Sec14p in a soluble protein-PtdCho complex. Phosphatidylcholines 126-145 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 164-170 17344474-1 2007 Molecular dynamics simulations coupled with functional analyses of the major yeast phosphatidylinositol/phosphatidylcholine transfer protein Sec14p identify structural elements involved in regulating the ability of Sec14p to execute phospholipid exchange. Phosphatidylcholines 104-123 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 141-147 17344474-1 2007 Molecular dynamics simulations coupled with functional analyses of the major yeast phosphatidylinositol/phosphatidylcholine transfer protein Sec14p identify structural elements involved in regulating the ability of Sec14p to execute phospholipid exchange. Phosphatidylcholines 104-123 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 215-221 17174597-0 2007 Phosphatidylcholine transfer activity of yeast Sec14p is not essential for its function in vivo. Phosphatidylcholines 0-19 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 47-53 17174597-1 2007 Yeast phosphatidylinositol (PI)/phosphatidylcholine (PC) transfer protein, Sec14p, is essential for protein transport from the Golgi apparatus and for the cell viability. Phosphatidylcholines 32-51 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 75-81 17174597-5 2007 Thus, in vitro PC transfer ability of Sec14p is not required for its essential function(s) in living cells, however, yeast cells having PC transfer deficient Sec14p(D115G) as a sole Sec14p display regulatory abnormalities, including increased phospholipase D mediated PC turnover. Phosphatidylcholines 15-17 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 38-44 17077504-1 2006 Sec14p is the major phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein in the budding yeast Saccharomyces cerevisiae and is the founding member of a large eukaryotic protein superfamily. Phosphatidylcholines 50-69 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-6 16845888-0 2006 Regulation of phosphatidylcholine homeostasis by Sec14. Phosphatidylcholines 14-33 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 49-54 16709158-1 2006 The major PI (phosphatidylinositol)/PC (phosphatidylcholine)-transfer protein in yeast, Sec14p, co-ordinates lipid metabolism with protein transport from the Golgi complex. Phosphatidylcholines 40-59 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 88-94 16845888-3 2006 Yeast studies have determined that the phosphatidylcholine and (or) phosphatidylinositol binding protein, Sec14, is a major regulator of phosphatidylcholine homeostasis. Phosphatidylcholines 39-58 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 106-111 16845888-3 2006 Yeast studies have determined that the phosphatidylcholine and (or) phosphatidylinositol binding protein, Sec14, is a major regulator of phosphatidylcholine homeostasis. Phosphatidylcholines 137-156 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 106-111 16845888-4 2006 Sec14 itself regulates vesicular transport from the Golgi, and the interrelationship between phosphatidylcholine metabolism and membrane movement within the cell is described in detail. Phosphatidylcholines 93-112 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-5 16137924-7 2005 A complex of PtdCho and Sec14p, a yeast phospholipid-binding protein, both inhibits the rate-limiting step in PtdCho synthesis and enhances deacylation of PtdCho by NTE. Phosphatidylcholines 13-19 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 24-30 12200438-8 2002 Pct1p activity is regulated by Sec14p, a cytoplasm/Golgi localized phosphatidylcholine/phosphatidylinositol binding protein that regulates Golgi-derived vesicle transport partially through its ligand-dependent regulation of PCT1 derived enzyme activity. Phosphatidylcholines 67-86 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 31-37 15611060-0 2005 Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p. Phosphatidylcholines 30-49 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 78-84 15611060-9 2005 Furthermore, our analyses revealed a profound alteration of PC metabolism triggered by the absence of Sec14p: Nte1p unresponsiveness, Spo14p activation, and deregulation of Pct1p. Phosphatidylcholines 60-62 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 102-108 15611060-12 2005 Beyond the new PC metabolic control features we ascribe to Sfh2p and Sfh4p we also describe a second role for Sec14p in mediating PC homeostasis. Phosphatidylcholines 130-132 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 110-116 12869188-7 2003 Interestingly, Sec14p and the two homologues Sfh2p and Sfh4p downregulate phospholipase B1 (Plb1p)-mediated turnover of phosphatidylcholine in vivo. Phosphatidylcholines 120-139 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 15-21 15617515-3 2005 Current data imply that the yeast ORP Kes1p is a negative regulator of Golgi-derived vesicular transport mediated by the essential phosphatidylinositol/phosphatidylcholine transfer protein Sec14p. Phosphatidylcholines 152-171 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 189-195 12749692-1 2003 Three human proteins (hTAP1, hTAP2 and hTAP3) that are related to the yeast phosphatidylinositol/phosphatidylcholine transfer protein SEC14p were recently cloned in our laboratory. Phosphatidylcholines 97-116 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 134-140 11916983-3 2002 Kes1p, one of seven members of the yeast OSBP family, negatively regulates Golgi complex secretory functions that are dependent on the action of the major yeast phosphatidylinositol/phosphatidylcholine Sec14p. Phosphatidylcholines 182-201 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 202-208 12134061-2 2002 This essential Sec14p requirement for Golgi function is bypassed by mutations in any one of seven genes that control phosphatidylcholine or phosphoinositide metabolism. Phosphatidylcholines 117-136 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 15-21 10799527-0 2000 A new gene involved in the transport-dependent metabolism of phosphatidylserine, PSTB2/PDR17, shares sequence similarity with the gene encoding the phosphatidylinositol/phosphatidylcholine transfer protein, SEC14. Phosphatidylcholines 169-188 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 207-212 11251067-0 2001 Phosphatidylcholine synthesis influences the diacylglycerol homeostasis required for SEC14p-dependent Golgi function and cell growth. Phosphatidylcholines 0-19 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 85-91 11251067-5 2001 Alterations in the level of diacylglycerol consumption through alterations in phosphatidylcholine synthesis directly correlated with the level of SEC14-dependent invertase secretion and affected cell viability. Phosphatidylcholines 78-97 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 146-151 11514437-7 2001 Choline and phosphatidic acid molecular species profiles during Sec14-independent secretion and meiosis reveal that while strains harboring one of these alleles, spo14S-11, hydrolyze phosphatidylcholine in Sec14-independent secretion, they fail to do so during sporulation or normal vegetative growth. Phosphatidylcholines 183-202 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 64-69 11514437-7 2001 Choline and phosphatidic acid molecular species profiles during Sec14-independent secretion and meiosis reveal that while strains harboring one of these alleles, spo14S-11, hydrolyze phosphatidylcholine in Sec14-independent secretion, they fail to do so during sporulation or normal vegetative growth. Phosphatidylcholines 183-202 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 206-211 11294911-0 2001 Evidence for an intrinsic toxicity of phosphatidylcholine to Sec14p-dependent protein transport from the yeast Golgi complex. Phosphatidylcholines 38-57 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 61-67 10799527-5 2000 The PSTB2 gene is allelic to the pleiotropic drug resistance gene, PDR17, and is homologous to SEC14, which encodes a phosphatidylinositol/phosphatidylcholine transfer protein. Phosphatidylcholines 139-158 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 95-100 9874205-1 1998 The SEC14 gene of Saccharomyces cerevisiae codes for a phosphatidylinositol-transfer protein (Sec14p(sc)) which is capable of transferring both phosphatidylinositol and phosphatidylcholine between membranes in vitro. Phosphatidylcholines 169-188 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 4-9 10397762-5 1999 This phosphatidylcholine metabolic phenotype is sensitive to the two physiological challenges that abolish bypass Sec14p in sac1 strains; i.e. phospholipase D inactivation and expression of bacterial diacylglycerol (DAG) kinase. Phosphatidylcholines 5-24 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 114-120 10224048-7 1999 Together, our data provide a long sought mechanism as to how defects in Sac1p overcome certain actin mutants and bypass the requirement for yeast phosphatidylinositol/phosphatidylcholine transfer protein, Sec14p. Phosphatidylcholines 167-186 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 205-211 9874205-1 1998 The SEC14 gene of Saccharomyces cerevisiae codes for a phosphatidylinositol-transfer protein (Sec14p(sc)) which is capable of transferring both phosphatidylinositol and phosphatidylcholine between membranes in vitro. Phosphatidylcholines 169-188 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 94-100 9642212-2 1998 Mutations (cki1, cct1, and cpt1) in the CDP-choline pathway for phosphatidylcholine synthesis suppress the sec14 growth defect (2), permitting sec14(ts) cki1, sec14(ts) cct1, and sec14(ts) cpt1 strains to grow at the sec14(ts) restrictive temperature. Phosphatidylcholines 64-83 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 107-112 9642212-2 1998 Mutations (cki1, cct1, and cpt1) in the CDP-choline pathway for phosphatidylcholine synthesis suppress the sec14 growth defect (2), permitting sec14(ts) cki1, sec14(ts) cct1, and sec14(ts) cpt1 strains to grow at the sec14(ts) restrictive temperature. Phosphatidylcholines 64-83 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 143-148 9642212-2 1998 Mutations (cki1, cct1, and cpt1) in the CDP-choline pathway for phosphatidylcholine synthesis suppress the sec14 growth defect (2), permitting sec14(ts) cki1, sec14(ts) cct1, and sec14(ts) cpt1 strains to grow at the sec14(ts) restrictive temperature. Phosphatidylcholines 64-83 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 143-148 9642212-2 1998 Mutations (cki1, cct1, and cpt1) in the CDP-choline pathway for phosphatidylcholine synthesis suppress the sec14 growth defect (2), permitting sec14(ts) cki1, sec14(ts) cct1, and sec14(ts) cpt1 strains to grow at the sec14(ts) restrictive temperature. Phosphatidylcholines 64-83 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 143-148 9642212-2 1998 Mutations (cki1, cct1, and cpt1) in the CDP-choline pathway for phosphatidylcholine synthesis suppress the sec14 growth defect (2), permitting sec14(ts) cki1, sec14(ts) cct1, and sec14(ts) cpt1 strains to grow at the sec14(ts) restrictive temperature. Phosphatidylcholines 64-83 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 143-148 9461221-1 1998 The yeast phosphatidylinositol-transfer protein (Sec14) catalyses exchange of phosphatidylinositol and phosphatidylcholine between membrane bilayers in vitro. Phosphatidylcholines 103-122 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 49-54 9139830-2 1997 This requirement can be relieved by inactivation of the cytosine 5"-diphosphate (CDP)-choline pathway for phosphatidylcholine biosynthesis, indicating that Sec14p is an essential component of a regulatory pathway linking phospholipid metabolism with vesicle trafficking (the Sec14p pathway). Phosphatidylcholines 106-125 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 156-162 9252414-0 1997 Role of the yeast phosphatidylinositol/phosphatidylcholine transfer protein (Sec14p) in phosphatidylcholine turnover and INO1 regulation. Phosphatidylcholines 39-58 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 77-83 9252414-1 1997 In yeast, mutations in the CDP-choline pathway for phosphatidylcholine biosynthesis permit the cell to grow even when the SEC14 gene is completely deleted (Cleves, A., McGee, T., Whitters, E., Champion, K., Aitken, J., Dowhan, W., Goebl, M., and Bankaitis, V. (1991) Cell 64, 789-800). Phosphatidylcholines 51-70 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 122-127 9252414-4 1997 We show that the increased choline excretion in sec14(ts) cki1 cells is due to increased turnover of phosphatidylcholine via a mechanism consistent with phospholipase D-mediated turnover. Phosphatidylcholines 101-120 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 48-53 9252414-5 1997 We propose that the elevated rate of phosphatidylcholine turnover in sec14(ts) cki1 cells provides the metabolic condition that permits the secretory pathway to function when Sec14p is inactivated. Phosphatidylcholines 37-56 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 69-74 9252414-5 1997 We propose that the elevated rate of phosphatidylcholine turnover in sec14(ts) cki1 cells provides the metabolic condition that permits the secretory pathway to function when Sec14p is inactivated. Phosphatidylcholines 37-56 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 175-181 9252414-6 1997 As phosphatidylcholine turnover increases in sec14(ts) cki1 cells shifted to the restrictive temperature, the INO1 gene (encoding inositol-1-phosphate synthase) is also derepressed, leading to an inositol excretion phenotype (Opi-). Phosphatidylcholines 3-22 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 45-50 8978672-1 1996 The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation of the CDP-choline pathway for phosphatidylcholine biosynthesis. Phosphatidylcholines 238-257 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 49-55 7816798-6 1995 Finally, whereas the SEC14p-dependent inhibition of CCTase in vitro was markedly reduced under assay conditions that were expected to increase levels of phosphatidylinositol-bound SEC14p, assay conditions expected to increase levels of phosphatidylcholine-bound SEC14p resulted in significant potentiation of CCTase inhibition. Phosphatidylcholines 236-255 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 21-27 7816798-7 1995 The collective data suggest that the phosphatidylcholine-bound form of SEC14p effects an essential repression of CDP-choline pathway activity in Golgi membranes by inhibiting CCTase and that the phospholipid-binding/exchange activity of SEC14p represents a mechanism by which the regulatory activity of SEC14p is itself controlled. Phosphatidylcholines 37-56 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 71-77 7816798-7 1995 The collective data suggest that the phosphatidylcholine-bound form of SEC14p effects an essential repression of CDP-choline pathway activity in Golgi membranes by inhibiting CCTase and that the phospholipid-binding/exchange activity of SEC14p represents a mechanism by which the regulatory activity of SEC14p is itself controlled. Phosphatidylcholines 37-56 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 237-243 7816798-7 1995 The collective data suggest that the phosphatidylcholine-bound form of SEC14p effects an essential repression of CDP-choline pathway activity in Golgi membranes by inhibiting CCTase and that the phospholipid-binding/exchange activity of SEC14p represents a mechanism by which the regulatory activity of SEC14p is itself controlled. Phosphatidylcholines 37-56 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 237-243 1390857-3 1992 Using the temperature-sensitive mutant strain Saccharomyces cerevisiae sec 14, which is defective in the phosphatidylinositol transfer protein, it could be demonstrated that this protein is not involved in the transport of phosphatidylinositol and phosphatidylcholine from internal membranes to the plasma membrane. Phosphatidylcholines 248-267 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 71-77 8294512-3 1994 The data demonstrate that SEC14p specifically functions to maintain a reduced phosphatidylcholine content in Golgi membranes and indicate that overproduction of SEC14p markedly reduces the apparent rate of phosphatidylcholine biosynthesis via the CDP-choline pathway in vivo. Phosphatidylcholines 78-97 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 26-32 8294512-3 1994 The data demonstrate that SEC14p specifically functions to maintain a reduced phosphatidylcholine content in Golgi membranes and indicate that overproduction of SEC14p markedly reduces the apparent rate of phosphatidylcholine biosynthesis via the CDP-choline pathway in vivo. Phosphatidylcholines 78-97 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 161-167 8294512-3 1994 The data demonstrate that SEC14p specifically functions to maintain a reduced phosphatidylcholine content in Golgi membranes and indicate that overproduction of SEC14p markedly reduces the apparent rate of phosphatidylcholine biosynthesis via the CDP-choline pathway in vivo. Phosphatidylcholines 206-225 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 26-32 8294512-3 1994 The data demonstrate that SEC14p specifically functions to maintain a reduced phosphatidylcholine content in Golgi membranes and indicate that overproduction of SEC14p markedly reduces the apparent rate of phosphatidylcholine biosynthesis via the CDP-choline pathway in vivo. Phosphatidylcholines 206-225 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 161-167 8294512-4 1994 We suggest that SEC14p serves as a sensor of Golgi membrane phospholipid composition through which the activity of the CDP-choline pathway in Golgi membranes is regulated such that a phosphatidylcholine content that is compatible with the essential secretory function of these membranes is maintained. Phosphatidylcholines 183-202 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 16-22 1997207-1 1991 SEC14p is the yeast phosphatidylinositol (PI)/phosphatidylcholine (PC) transfer protein, and it effects an essential stimulation of yeast Golgi secretory function. Phosphatidylcholines 46-65 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 0-6 1997207-4 1991 The antagonistic action of the CKI gene product on SEC14p function revealed a previously unsuspected influence of biosynthetic activities of the CDP-choline pathway for PC biosynthesis on yeast Golgi function and indicated that SEC14p controls the phospholipid content of yeast Golgi membranes in vivo. Phosphatidylcholines 169-171 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 Saccharomyces cerevisiae S288C 51-57