PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 14994387-4 2004 IFN-gamma and IL-4 production by CD4+ T cells stimulated with phorbol myristate acetate (PMA) and calcium ionophore A23187 was measured by intracellular cytokine staining and flow cytometry. Calcimycin 116-122 interferon gamma Homo sapiens 0-9 16278302-4 2006 beta-agonist and PGE2 also inhibited phorbol myristate acetate (PMA) + calcimycin-stimulated IFN-gamma and IL-2 (but not IL-13) production, suggesting that upstream CD3-initiated signaling is not the sole locus of PKA actions. Calcimycin 71-81 interferon gamma Homo sapiens 93-102 16141532-7 2005 Interestingly, A23187 synergized with the activities of CKBM-treated THP-1 cells in terms of IL-1beta and IFNgamma production, while the IL-10 production showed no synergistic relationship between CKBM and A23187. Calcimycin 15-21 interferon gamma Homo sapiens 106-114 14994387-11 2004 PMA and A23187-activated ST CD4+ T cells mostly expressed IL-18Ralpha and produced high levels of IFN-gamma. Calcimycin 8-14 interferon gamma Homo sapiens 98-107 8113394-5 1994 Calcium ionophore A23187 (10(-5) M) further increased the [3H]AA release from the IFN-gamma-treated cells. Calcimycin 18-24 interferon gamma Homo sapiens 82-91 9751101-5 1998 RESULTS: When ST cells were stimulated with PMA plus A23187 in bulk culture, IFNgamma-producing T cells were more frequently detected in the CD8+ subset, but cells producing other cytokines were found in the CD4+ subset. Calcimycin 53-59 interferon gamma Homo sapiens 77-85 9751101-6 1998 Purified ST CD4+ T cells, after stimulation with PMA plus A23187, were able to produce higher levels of IFNgamma but lower levels of IL-4 and IL-13, by analysis at the single-cell level, as compared with the PB CD4+, CD45RO+ T cells. Calcimycin 58-64 interferon gamma Homo sapiens 104-112 10353535-8 1999 Both calcium ionophore, A23187, and the protein kinase C (PKC) activator phorbol-myristate-acetate (PMA) had a synergistic effect on IFN-gamma-induced EC apoptosis (p < .05). Calcimycin 24-30 interferon gamma Homo sapiens 133-142 8977524-4 1996 The tyrosine kinase inhibitor herbimycin inhibited the production of interferon-gamma (IFN-gamma) by THO-like clone, after stimulation with anti-CD3 monoclonal antibody alpha CD3-mAb) or with phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore A23187. Calcimycin 256-262 interferon gamma Homo sapiens 69-85 8977524-4 1996 The tyrosine kinase inhibitor herbimycin inhibited the production of interferon-gamma (IFN-gamma) by THO-like clone, after stimulation with anti-CD3 monoclonal antibody alpha CD3-mAb) or with phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore A23187. Calcimycin 256-262 interferon gamma Homo sapiens 87-96 8977524-7 1996 A23187, which synergizes with PMA in the induction of IL-4 and IFN-gamma, inhibited PMA-induced IL-10 production in a dose-dependent manner. Calcimycin 0-6 interferon gamma Homo sapiens 63-72 8870699-7 1996 Activation of PPD-specific TCL from patients with calcium ionophore A23187 plus phorbol myristate acetate resulted in much higher IFN-gamma production than in TCL established from healthy controls, indicating that the low production of IFN-gamma by PPD-specific T cells from atopic patients is not due to an intrinsic T cell defect but to some regulatory mechanisms. Calcimycin 68-74 interferon gamma Homo sapiens 130-139 8870699-7 1996 Activation of PPD-specific TCL from patients with calcium ionophore A23187 plus phorbol myristate acetate resulted in much higher IFN-gamma production than in TCL established from healthy controls, indicating that the low production of IFN-gamma by PPD-specific T cells from atopic patients is not due to an intrinsic T cell defect but to some regulatory mechanisms. Calcimycin 68-74 interferon gamma Homo sapiens 236-245 8964081-2 1996 One of these CD8+ T cell clones, 13G2, secreted IFN-gamma at similar levels with calcium ionophore, A23187, as well as by Con A, but IL-10 production by A23187 was less than by Con A. Calcimycin 100-106 interferon gamma Homo sapiens 48-57 8762005-10 1996 Likewise, the induction of IFN-gamma and IL-2 mRNA by calcium ionophore A23187 was inhibited with IC50 of 14 ng/ml (95% CI = 8-27 ng/ml) and 32 ng/ml (95% CI = 5-178 ng/ml), respectively, while the IC50 for inhibition of IFN-gamma protein secretion was 8 ng/ml (95% CI = 9-18 ng/ml). Calcimycin 72-78 interferon gamma Homo sapiens 27-36 8762005-10 1996 Likewise, the induction of IFN-gamma and IL-2 mRNA by calcium ionophore A23187 was inhibited with IC50 of 14 ng/ml (95% CI = 8-27 ng/ml) and 32 ng/ml (95% CI = 5-178 ng/ml), respectively, while the IC50 for inhibition of IFN-gamma protein secretion was 8 ng/ml (95% CI = 9-18 ng/ml). Calcimycin 72-78 interferon gamma Homo sapiens 221-230 7583919-4 1995 PMA at a concentration of 50 ng/ml plus 50 ng of calcium ionophore A23187 per ml was used to induce IFN-gamma, while 150 hemagglutinating units of Sendai virus was used to induce IFN-alpha production. Calcimycin 67-73 interferon gamma Homo sapiens 100-103 7974918-4 1994 The calcium ionophore, 4-bromo-calcium ionophore A23187 (Bromo-A23187) significantly enhanced the IFN-gamma and PMA effect. Calcimycin 49-55 interferon gamma Homo sapiens 98-107 7684703-5 1993 In addition, it was shown that the effects of IFN-gamma on LPS-induced G-CSF protein secretion could be mimicked by the calcium ionophore A23187, suggesting that the Ca(2+)-dependent pathway might be triggered after binding of the ligand to the receptor. Calcimycin 138-144 interferon gamma Homo sapiens 46-55 8140295-4 1993 Our results show that SEA- and A23187-stimulated PBMC from patients with S released significantly less IFN-gamma than those from control subjects. Calcimycin 31-37 interferon gamma Homo sapiens 103-112 8103344-3 1993 Calcium ionophore A23187 significantly enhanced IFN-gamma- and PMA-induced ICAM-1 staining. Calcimycin 18-24 interferon gamma Homo sapiens 48-57 3113436-1 1987 Human peripheral blood mononuclear leukocytes were found to produce large amounts of interferon-gamma in response to the calcium ionophore A23187 combined with the phorbol ester mezerein. Calcimycin 139-145 interferon gamma Homo sapiens 85-101 8384238-3 1993 Following pretreatment of human PMNs with recombinant IFN-gamma, superoxide anion release was selectively primed toward the receptor-initiated stimulants f-Met-Leu-Phe (fMLP) and C5a but not toward the transduction-mediated stimulants phorbol myristate acetate and A23187, a calcium ionophore. Calcimycin 265-271 interferon gamma Homo sapiens 54-63 8419187-4 1993 After stimulation with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187, they produced higher levels of IL-4 (306 vs. 55 +/- 4 pg/ml) and IL-5 (2900 vs. 213 +/- 72 pg/ml) and lower levels of IL-2 (17 vs. 63 +/- 17 IU/ml) and interferon-gamma (IFN-gamma) (16 vs. 299 +/- 70 IU/ml) than controls CD4+ CD45R0+ cells. Calcimycin 83-89 interferon gamma Homo sapiens 243-259 8419187-4 1993 After stimulation with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187, they produced higher levels of IL-4 (306 vs. 55 +/- 4 pg/ml) and IL-5 (2900 vs. 213 +/- 72 pg/ml) and lower levels of IL-2 (17 vs. 63 +/- 17 IU/ml) and interferon-gamma (IFN-gamma) (16 vs. 299 +/- 70 IU/ml) than controls CD4+ CD45R0+ cells. Calcimycin 83-89 interferon gamma Homo sapiens 261-270 1370514-8 1992 Stimulation with Con A also induced very low or no measurable levels of IL-2 and IFN-gamma, whereas activation with TPA and the calcium ionophore A23187 resulted in the production of high levels of IL-4, IL-5, IL-2, and IFN-gamma. Calcimycin 146-152 interferon gamma Homo sapiens 220-229 1901945-1 1991 Treatment of macrophages with interferon-gamma (IFN gamma) strongly decreased the induction of c-fos mRNA by 12-O-tetradecanoylphorbol-13-acetate (TPA), lipopolysaccharide, or calcium ionophore A23187 in macrophages. Calcimycin 194-200 interferon gamma Homo sapiens 30-46 1901945-1 1991 Treatment of macrophages with interferon-gamma (IFN gamma) strongly decreased the induction of c-fos mRNA by 12-O-tetradecanoylphorbol-13-acetate (TPA), lipopolysaccharide, or calcium ionophore A23187 in macrophages. Calcimycin 194-200 interferon gamma Homo sapiens 48-57 2507204-0 1989 Interleukin 2, interleukin 2 receptor, and interferon-gamma synthesis and mRNA expression in phorbol myristate acetate and calcium ionophore A23187-stimulated T cells from elderly humans. Calcimycin 141-147 interferon gamma Homo sapiens 43-59 2969394-3 1988 PBL, stimulated with Con A or with a combination of the phorbol ester 13-O-tetradecanoylphorbol-12-acetate and the Ca2+ ionophore A23187 secreted IL-2, IL-4, and IFN-gamma. Calcimycin 130-136 interferon gamma Homo sapiens 162-171 3132511-1 1988 A23187 in combination with phorbol myristate acetate (PMA) strongly induces production of interferon-gamma (IFN-gamma) by human peripheral blood mononuclear cells (PBMC) and even by murine PBMC, which respond poorly to A23187 alone. Calcimycin 0-6 interferon gamma Homo sapiens 90-106 3132511-1 1988 A23187 in combination with phorbol myristate acetate (PMA) strongly induces production of interferon-gamma (IFN-gamma) by human peripheral blood mononuclear cells (PBMC) and even by murine PBMC, which respond poorly to A23187 alone. Calcimycin 0-6 interferon gamma Homo sapiens 108-117 3132511-1 1988 A23187 in combination with phorbol myristate acetate (PMA) strongly induces production of interferon-gamma (IFN-gamma) by human peripheral blood mononuclear cells (PBMC) and even by murine PBMC, which respond poorly to A23187 alone. Calcimycin 219-225 interferon gamma Homo sapiens 90-106 3132511-1 1988 A23187 in combination with phorbol myristate acetate (PMA) strongly induces production of interferon-gamma (IFN-gamma) by human peripheral blood mononuclear cells (PBMC) and even by murine PBMC, which respond poorly to A23187 alone. Calcimycin 219-225 interferon gamma Homo sapiens 108-117 3123367-3 1987 In our experiments, we investigated the influence of bryostatin 1 on the synthesis of interleukin 2 (IL2) and interferon-gamma (IFN-gamma) by ionophore A23187 or mitogen-induced human blood lymphocytes. Calcimycin 152-158 interferon gamma Homo sapiens 110-126 3123367-3 1987 In our experiments, we investigated the influence of bryostatin 1 on the synthesis of interleukin 2 (IL2) and interferon-gamma (IFN-gamma) by ionophore A23187 or mitogen-induced human blood lymphocytes. Calcimycin 152-158 interferon gamma Homo sapiens 128-137 3115598-4 1987 However, activation by the phorbol ester PMA in conjunction with either PHA or the calcium ionophore A23187 induces large amounts of IFN-gamma and IL-2. Calcimycin 101-107 interferon gamma Homo sapiens 133-142 1310709-1 1992 Calcium ionophore A23187 can mimic IFN-gamma-induced macrophage activation for intracellular Leishmania killing and secretion of L-arginine-derived nitrite. Calcimycin 18-24 interferon gamma Homo sapiens 35-44 1310709-5 1992 The addition of exogenous PGE2, of agents increasing PGE2 production such as arachidonic acid and colchicine, or of an analogue of cAMP, dibutyryl cAMP inhibited A23187 + LPS-induced activation whereas that mediated by IFN-gamma + LPS remained unimpaired. Calcimycin 162-168 interferon gamma Homo sapiens 219-228 1431547-4 1992 In response to 10 microM calcium ionophore A23187 incubation (30 min), undifferentiated and IFN-gamma-differentiated HL60 cells formed both cyclooxygenase products (thromboxane and prostaglandins) and lipoxygenase products (leukotrienes and hydroxyeicosatetraenoic acids). Calcimycin 43-49 interferon gamma Homo sapiens 92-101 1908004-7 1991 However, when A23187 was added to high dose (1:120) PHA-stimulated cord blood MNCs IFN-gamma production increased to levels comparable with adult PHA-stimulated MNCs. Calcimycin 14-20 interferon gamma Homo sapiens 83-92 2156322-3 1990 We hypothesized that U937 and THP-1 cells would release LTB4, LTC4, and LTD4 in response to stimulation with the non-physiologic agonist, calcium ionophore A23187 and that preincubation with IFN-gamma or PMA might alter leukotriene release by these cells. Calcimycin 156-162 interferon gamma Homo sapiens 191-200 3113436-3 1987 A23187 plus mezerein induced high levels of interleukin-2 and interferon-gamma mRNAs. Calcimycin 0-6 interferon gamma Homo sapiens 62-78 3099131-3 1986 Using this improved method we demonstrated that reagents such as 12-O-tetradecanoylphorbol-13-acetate (TPA), concanavalin A (Con A) and a calcium ionophore (A23187) could induce the expression of IFN-gamma gene of a human T-lymphoblastoid cell line, TCL-Fuj 2M, where TPA functioned synergistically with Con A and A23187. Calcimycin 157-163 interferon gamma Homo sapiens 196-205 3099131-3 1986 Using this improved method we demonstrated that reagents such as 12-O-tetradecanoylphorbol-13-acetate (TPA), concanavalin A (Con A) and a calcium ionophore (A23187) could induce the expression of IFN-gamma gene of a human T-lymphoblastoid cell line, TCL-Fuj 2M, where TPA functioned synergistically with Con A and A23187. Calcimycin 314-320 interferon gamma Homo sapiens 196-205 3021873-5 1986 Addition of the calcium ionophore A23187 partially restored IFN-gamma production. Calcimycin 34-40 interferon gamma Homo sapiens 60-69 3923610-5 1985 On the contrary, the calcium ionophore A23187 (considered to act as a second specific step, following oxidation of galactose residues, toward genetic derepression of IFN-gamma) induced considerable IFN-gamma production in all the three tested patients. Calcimycin 39-45 interferon gamma Homo sapiens 166-175 3923610-5 1985 On the contrary, the calcium ionophore A23187 (considered to act as a second specific step, following oxidation of galactose residues, toward genetic derepression of IFN-gamma) induced considerable IFN-gamma production in all the three tested patients. Calcimycin 39-45 interferon gamma Homo sapiens 198-207