PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
11101309-0	2000	Intrinsic fluorescence of the P-glycoprotein multidrug transporter: sensitivity of tryptophan residues to binding of drugs and nucleotides.	Tryptophan	83-93	ATP binding cassette subfamily B member 1	Homo sapiens	30-44	
12034727-10	2002	[125I]LY475776 labeling of mutant MRP1 molecules with substitutions of Trp(1246) in TM17 were reduced >80% compared with wild-type MRP1, confirming that TM17 is important for LY475776 binding.	Tryptophan	71-74	ATP binding cassette subfamily B member 1	Homo sapiens	34-38	
11278867-0	2001	Mutation of a single conserved tryptophan in multidrug resistance protein 1 (MRP1/ABCC1) results in loss of drug resistance and selective loss of organic anion transport.	Tryptophan	31-41	ATP binding cassette subfamily B member 1	Homo sapiens	45-75	
11925441-2	2002	We previously demonstrated that two hydrogen-bonding amino acid residues in the predicted transmembrane 17 (TM17) of MRP1, Thr(1242) and Trp(1246), were important for drug resistance and 17beta-estradiol 17-(beta-d-glucuronide) (E(2)17betaG) transport.	Tryptophan	137-140	ATP binding cassette subfamily B member 1	Homo sapiens	117-121	
11101309-3	2000	Steady-state and lifetime fluorescence techniques were used to probe the environment of the 11 tryptophan residues within purified functional P-glycoprotein, and their response to binding of nucleotides and substrates.	Tryptophan	95-105	ATP binding cassette subfamily B member 1	Homo sapiens	142-156	
11101309-7	2000	P-glycoprotein fluorescence was highly quenched on binding of fluorescent nucleotides, and moderately quenched by ATP, ADP, and AMP-PNP, suggesting that the site for nucleotide binding is located relatively close to tryptophan residues.	Tryptophan	216-226	ATP binding cassette subfamily B member 1	Homo sapiens	0-14	
35634783-5	2022	Here, we report a system for measuring the affinity of substrate binding to the Cyanidioschyzon merolae ABCB1 (CmABCB1) using site-specific tryptophan (Trp) fluorescence quenching.	Tryptophan	140-150	ATP binding cassette subfamily B member 1	Homo sapiens	104-109	
10364203-3	1999	Trp quenching experiments provided evidence that P-glycoprotein adopts different tertiary structures upon binding of drug substrates in the absence and presence of MgATP and its nonhydrolyzable analog, MgATPgammaS.	Tryptophan	0-3	ATP binding cassette subfamily B member 1	Homo sapiens	49-63	
35634783-5	2022	Here, we report a system for measuring the affinity of substrate binding to the Cyanidioschyzon merolae ABCB1 (CmABCB1) using site-specific tryptophan (Trp) fluorescence quenching.	Tryptophan	152-155	ATP binding cassette subfamily B member 1	Homo sapiens	104-109	
29499278-6	2018	Acrylamide quenching of tryptophan fluorescence to probe Pgp conformational changes revealed that methadone- and loperamide-induced conformational changes were distinct.	Tryptophan	24-34	ATP binding cassette subfamily B member 1	Homo sapiens	57-60	
19398503-3	2009	We have previously shown that mutations of Lys(332) in transmembrane helix (TM) 6 and Trp(1246) in TM17 cause different substrate-selective losses in MRP1 transport activity.	Tryptophan	86-89	ATP binding cassette subfamily B member 1	Homo sapiens	150-154	
17490606-4	2007	Quenching of the intrinsic Trp fluorescence of purified P-glycoprotein was used to quantitate insecticide binding; the estimated K(d) values fell in the range 4-6 microM.	Tryptophan	27-30	ATP binding cassette subfamily B member 1	Homo sapiens	56-70	
16919386-2	2007	In the present study tryptophan intrinsic fluorescence was used to understand the P-glycoprotein response to three benzodiazepines (bromazepam, chlordiazepoxide and flurazepam) in the presence and absence of ATP.	Tryptophan	21-31	ATP binding cassette subfamily B member 1	Homo sapiens	82-96	
19818729-0	2009	Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study.	Tryptophan	71-74	ATP binding cassette subfamily B member 1	Homo sapiens	53-67	
19818729-2	2009	Intrinsic Trp fluorescence was used to probe the interactions of LDS-751 (2-[4-(4-[dimethylamino]phenyl)-1,3-butadienyl]-3-ethylbenzo-thiazolium perchlorate) with purified P-glycoprotein, using steady-state/lifetime measurements and collisional quenching.	Tryptophan	10-13	ATP binding cassette subfamily B member 1	Homo sapiens	172-186	
17257580-3	2007	We investigated the structure and dynamics of predicted transmembrane fragment 17 (TM17, from Ala(1227) to Ser(1251)), which contains a single Trp residue (W(1246)) involved in MRP1 substrate specificity and transport function.	Tryptophan	143-146	ATP binding cassette subfamily B member 1	Homo sapiens	177-181	
15641790-6	2005	LDS-751 bound with sub-micromolar affinity (K(d) = 0.75 microM) and quenched P-glycoprotein intrinsic Trp fluorescence by 40%, suggesting that Trp emitters are probably located close to the drub-binding regions of the transporter and may interact directly with the dye.	Tryptophan	102-105	ATP binding cassette subfamily B member 1	Homo sapiens	77-91