PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30217652-6 2019 By using xCELLigence system, we demonstrated that mifepristone enhances toxicity of etoposide in a dose dependent manner with concomitant caspase-3 activity. Etoposide 84-93 caspase 3 Homo sapiens 138-147 30798416-5 2019 Nuclear staining with Hoechst 33258 and annexin V-FITC/PI, as well as analysis of caspase 3 activity, mitochondrial membrane potential, and apoptotic protein expression, showed that etoposide-induced apoptosis was attenuated by ANKRD49 overexpression but promoted by RNA interference-induced ANKRD49 knockdown. Etoposide 182-191 caspase 3 Homo sapiens 82-91 29678622-10 2018 TRIM8 stabilizes the turnover of XIAP during genotoxic stress and forms complex with XIAP and caspase-3 to inhibit its activation in presence of etoposide. Etoposide 145-154 caspase 3 Homo sapiens 94-103 30149619-0 2018 Etoposide-Induced Apoptosis in Cancer Cells Can Be Reinforced by an Uncoupled Link between Hsp70 and Caspase-3. Etoposide 0-9 caspase 3 Homo sapiens 101-110 30149619-8 2018 Competitive protein-protein interaction and immunoprecipitation assays showed that BT44 caused dissociation of the Hsp70-Caspase-3 complex, thus augmenting the anti-tumor activity of etoposide and highlighting the potential role of molecular separators in cancer therapy. Etoposide 183-192 caspase 3 Homo sapiens 121-130 30483138-0 2018 Lowering Etoposide Doses Shifts Cell Demise From Caspase-Dependent to Differentiation and Caspase-3-Independent Apoptosis via DNA Damage Response, Inducing AML Culture Extinction. Etoposide 9-18 caspase 3 Homo sapiens 90-99 30483138-3 2018 We observed that on a set of myeloid leukemia cell lines, etoposide at high (50 uM) dose promoted a rapid caspase-3-mediated apoptosis, whereas at low (0.5 uM) dose, it induced morphological and functional granulocytic differentiation and caspase-2-dependent, but caspase-3-independent, cell death, displaying features consistent with apoptosis. Etoposide 58-67 caspase 3 Homo sapiens 106-115 30483138-3 2018 We observed that on a set of myeloid leukemia cell lines, etoposide at high (50 uM) dose promoted a rapid caspase-3-mediated apoptosis, whereas at low (0.5 uM) dose, it induced morphological and functional granulocytic differentiation and caspase-2-dependent, but caspase-3-independent, cell death, displaying features consistent with apoptosis. Etoposide 58-67 caspase 3 Homo sapiens 264-273 28395566-2 2018 Here, we found in chemotherapeutic drug etoposide-induced apoptosis, WT1 protein was cleaved into smaller fragment by caspase-3 in leukemic cells. Etoposide 40-49 caspase 3 Homo sapiens 118-127 29030066-3 2018 In this study, we found that etoposide induces proliferation suppression, cell apoptosis and increases caspase-3 activity in A549 cells. Etoposide 29-38 caspase 3 Homo sapiens 103-112 28736504-10 2017 Importantly, these cells were more tolerant to cisplatin and etoposide treatment, showed a lower level of nuclear Caspase-3 in treated cells and harbored drug resistant CSCs. Etoposide 61-70 caspase 3 Homo sapiens 114-123 27666530-7 2016 Studies using MAPK inhibitors reveal that inhibition of extracellular signal regulated kinase (ERK) protects ETO-induced cytotoxicity by inhibiting DNA damage and caspase 3/7 activity. Etoposide 109-112 caspase 3 Homo sapiens 163-172 27925196-7 2017 Our results demonstrate that SIRT1-silenced cells are more resistant to H2 O2 and etoposide treatment showing decreased ROS accumulation, gamma-H2AX phosphorylation, caspase-3 activation and PARP cleavage. Etoposide 82-91 caspase 3 Homo sapiens 166-175 26622554-6 2015 Furthermore, the caspase-3, caspase-8 and caspase-9 activities in etoposide-induced apoptosis demonstrated a greater increase upon neuraminidase pretreatment compared with no neuraminidase pretreatment. Etoposide 66-75 caspase 3 Homo sapiens 17-26 26858249-5 2016 Ablation of c-Jun/c-Fos or ID1 expression enhanced etoposide-mediated apoptosis through increasing activity of caspase 3 and PARP cleavage. Etoposide 51-60 caspase 3 Homo sapiens 111-120 26202083-0 2015 Bcl10 crucially nucleates the pro-apoptotic complexes comprising PDK1, PKCzeta and caspase-3 at the nuclear envelope of etoposide-treated human cervical carcinoma C4-I cells. Etoposide 120-129 caspase 3 Homo sapiens 83-92 27035096-6 2016 Combination of PA with Eto/Cpt promoted disruption of the dynamics of actin filaments, leading to subsequent enhancement of apoptotic cell death via induction of caspase-3, -8, and -9, accompanied by increased phosphorylation of p38. Etoposide 23-26 caspase 3 Homo sapiens 162-183 26895377-4 2016 Overexpression of miR-30e in HCT116 cells not only inhibited gammaIR-, etoposide- or miR-34a-induced caspase-3-like DEVDase activities and cell death, but greatly accelerated and augmented their senescent phenotype. Etoposide 71-80 caspase 3 Homo sapiens 101-110 26191229-8 2015 Stable Bcl-2 overexpression led to reduced apoptosis rates as well as Cyt-C and Caspase-3 expressions in Jurkat cells after VP-16 application, which was similar in leucocytes of remission patients. Etoposide 124-129 caspase 3 Homo sapiens 80-89 23415866-7 2013 Interestingly, overexpression of either PICOT wild type or the D101A/D226A double point mutant accelerated etoposide-induced activation of caspase-3 whereas siRNA-mediated knockdown of PICOT blocked this phenomenon. Etoposide 107-116 caspase 3 Homo sapiens 139-148 24038446-0 2015 Phosphorylation of Smac by Akt promotes the caspase-3 activation during etoposide-induced apoptosis in HeLa cells. Etoposide 72-81 caspase 3 Homo sapiens 44-53 21617849-3 2011 In the present study, we observed that aspirin caused G0/G1 phase cell cycle arrest and reduced etoposide induced caspase-3 activation in hepatocellular carcinoma G2 (HepG2) cells. Etoposide 96-105 caspase 3 Homo sapiens 114-123 22528748-6 2012 The FRET-based CE system is composed of a homemade CE system and a laser source for detecting the dynamics of caspase-3 in various cells expressing sensors of caspase-3 that have been treated with anticancer drugs, such as cell cycle-independent drug cisplatin and specific cell cycle drugs camptothecin and etoposide, as well as their combination with tumor necrosis factor (TNF). Etoposide 308-317 caspase 3 Homo sapiens 110-119 22528748-6 2012 The FRET-based CE system is composed of a homemade CE system and a laser source for detecting the dynamics of caspase-3 in various cells expressing sensors of caspase-3 that have been treated with anticancer drugs, such as cell cycle-independent drug cisplatin and specific cell cycle drugs camptothecin and etoposide, as well as their combination with tumor necrosis factor (TNF). Etoposide 308-317 caspase 3 Homo sapiens 159-168 21646862-9 2011 Caspase-3 activation and pyknosis induced by two other pro-apoptotic stimuli, MK801 and etoposide, were likewise found to be associated with Beclin 1-independent autophagy and reduced by the knockdown of Atg7 but not Beclin 1. Etoposide 88-97 caspase 3 Homo sapiens 0-9 23124518-3 2012 In addition, sequential treatment with etoposide and TRAIL increased caspases 8, 9 and 3 activation, Mcl-1 cleavage and Bid truncation, which suggests that the ability of etoposide and TRAIL to induce apoptosis is mediated through activation of an intrinsic signalling pathway. Etoposide 39-48 caspase 3 Homo sapiens 69-88 23124518-3 2012 In addition, sequential treatment with etoposide and TRAIL increased caspases 8, 9 and 3 activation, Mcl-1 cleavage and Bid truncation, which suggests that the ability of etoposide and TRAIL to induce apoptosis is mediated through activation of an intrinsic signalling pathway. Etoposide 171-180 caspase 3 Homo sapiens 69-88 21468663-4 2011 In this study, we showed that etoposide treatment activated caspase-8 and caspase-3, leading to cleavages of p53, Bid and PARP, which subsequently induced apoptosis. Etoposide 30-39 caspase 3 Homo sapiens 74-83 21801448-6 2011 In addition to p53 restoration, caspase-8 reconstitution was needed for sensitization to etoposide-induced apoptosis, mitochondria depolarization, and cleavage of the procaspases-3, and -9. Etoposide 89-98 caspase 3 Homo sapiens 167-188 21554433-10 2011 Insulin addition to the culture medium reduced p53 phosphorylation, caspase-3 and caspase-9 cleavage, and caspase-3 enzymatic activity induced by etoposide. Etoposide 146-155 caspase 3 Homo sapiens 106-115 20697588-2 2010 A sensitive apoptotic analysis method is integrated into this microfluidic system for studying apoptosis of HeLa cells under the influence of anticancer drug, etoposide, with various concentrations in parallel; it measures the yellow fluorescent proteincyan fluorescent protein fluorescence resonance energy transfer (FRET) signal that responds to the activation of caspase-3, an indicator of cell apoptosis. Etoposide 159-168 caspase 3 Homo sapiens 366-375 20865015-4 2011 Further studies showed that INrf2 enhanced etoposide-mediated accumulation of Bax, increased release of cytochrome c from mitochondria, activated caspase-3/7, and enhanced DNA fragmentation and apoptosis. Etoposide 43-52 caspase 3 Homo sapiens 146-155 22216167-3 2011 Here we show that exogenous caspase-3 sensitizes MCF-7 and HBL100 breast cancers cells to chemotherapeutic treatments such as etoposide and methotrexate whereas co-transfection with caspase-3s strongly inhibits etoposide and methotrexate-induced apoptosis underlying thus the anti-apoptotic role of caspase-3s. Etoposide 126-135 caspase 3 Homo sapiens 28-37 22216167-3 2011 Here we show that exogenous caspase-3 sensitizes MCF-7 and HBL100 breast cancers cells to chemotherapeutic treatments such as etoposide and methotrexate whereas co-transfection with caspase-3s strongly inhibits etoposide and methotrexate-induced apoptosis underlying thus the anti-apoptotic role of caspase-3s. Etoposide 211-220 caspase 3 Homo sapiens 28-37 22216167-3 2011 Here we show that exogenous caspase-3 sensitizes MCF-7 and HBL100 breast cancers cells to chemotherapeutic treatments such as etoposide and methotrexate whereas co-transfection with caspase-3s strongly inhibits etoposide and methotrexate-induced apoptosis underlying thus the anti-apoptotic role of caspase-3s. Etoposide 211-220 caspase 3 Homo sapiens 182-191 22216167-4 2011 In caspase-3 transfected cells, lamin-A and alpha-fodrin were cleaved when caspase-3 was activated by etoposide or methotrexate. Etoposide 102-111 caspase 3 Homo sapiens 3-12 22216167-4 2011 In caspase-3 transfected cells, lamin-A and alpha-fodrin were cleaved when caspase-3 was activated by etoposide or methotrexate. Etoposide 102-111 caspase 3 Homo sapiens 75-84 22216167-6 2011 Depletion of caspase-3 by RNA interference in HBL100 containing endogenous caspase-3s caused reduction in etoposide and methotrexate-induced apoptosis, whereas the depletion of caspase-3s sensitized cells to chemotherapy. Etoposide 106-115 caspase 3 Homo sapiens 13-22 22216167-6 2011 Depletion of caspase-3 by RNA interference in HBL100 containing endogenous caspase-3s caused reduction in etoposide and methotrexate-induced apoptosis, whereas the depletion of caspase-3s sensitized cells to chemotherapy. Etoposide 106-115 caspase 3 Homo sapiens 75-84 22216167-6 2011 Depletion of caspase-3 by RNA interference in HBL100 containing endogenous caspase-3s caused reduction in etoposide and methotrexate-induced apoptosis, whereas the depletion of caspase-3s sensitized cells to chemotherapy. Etoposide 106-115 caspase 3 Homo sapiens 75-84 20955597-13 2010 We further show that down-regulation of caspase 3 via overexpression of RASSF1C reduces breast cancer cells" sensitivity to the apoptosis inducing agent, etoposide. Etoposide 154-163 caspase 3 Homo sapiens 40-49 20094062-5 2010 Detailed kinetic analysis of cells lacking cathepsin B or L or treated with the cysteine protease inhibitor, E64d, revealed a delay in these cells in etoposide- and IL-3 deprivation-induced caspase-3 activation and apoptosis induction but not clonogenic survival, indicating that cathepsins amplify rather than initiate apoptosis. Etoposide 150-159 caspase 3 Homo sapiens 190-199 19426747-4 2010 Induction of apoptosis in G-292 human osteoblastic cells by exposure to etoposide or the inflammatory cytokine TNF-alpha promoted acute caspase-3/7 activity and this increased activity was inhibited by pretreatment with estradiol. Etoposide 72-81 caspase 3 Homo sapiens 136-145 19734716-3 2009 This compound inhibited caspase- 3 induction in U937 cells with an IC(50) value of 40 microM after 8 h of etoposide treatment. Etoposide 106-115 caspase 3 Homo sapiens 24-34 19549763-0 2009 Etoposide induces protein kinase Cdelta- and caspase-3-dependent apoptosis in neuroblastoma cancer cells. Etoposide 0-9 caspase 3 Homo sapiens 45-54 19549763-2 2009 Etoposide induces the caspase-3-dependent cleavage of PKCdelta to its active p40 fragment, and active PKCdelta triggers the processing of caspase-3 by a positive-feedback mechanism. Etoposide 0-9 caspase 3 Homo sapiens 22-31 19794957-10 2009 siRNA-mediated silencing of c-jun increased the responsiveness of cells to etoposide under hypoxia, as shown by an increase in caspase 3 activity and lactate dehydrogenase release. Etoposide 75-84 caspase 3 Homo sapiens 127-136 19549763-2 2009 Etoposide induces the caspase-3-dependent cleavage of PKCdelta to its active p40 fragment, and active PKCdelta triggers the processing of caspase-3 by a positive-feedback mechanism. Etoposide 0-9 caspase 3 Homo sapiens 138-147 19549763-3 2009 Treatment of cells with the caspase-3-specific inhibitor N-benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethyl ketone or caspase-3-specific small interacting RNA (siRNA) prevented the etoposide-induced activation of caspase-8 and inhibited apoptosis. Etoposide 179-188 caspase 3 Homo sapiens 28-37 19549763-3 2009 Treatment of cells with the caspase-3-specific inhibitor N-benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethyl ketone or caspase-3-specific small interacting RNA (siRNA) prevented the etoposide-induced activation of caspase-8 and inhibited apoptosis. Etoposide 179-188 caspase 3 Homo sapiens 116-125 19549763-7 2009 Finally, we show that rottlerin blocks etoposide-induced apoptosis by inhibiting the PKCdelta-mediated activation of caspase-3 and by degrading caspase-2, which prevents caspase-8 activation. Etoposide 39-48 caspase 3 Homo sapiens 117-126 19655264-5 2009 We observed that caspases-3, -8, -9 and pan caspase inhibitors resulted in significant inhibition of etoposide-induced DNA fragmentation. Etoposide 101-110 caspase 3 Homo sapiens 17-35 18492488-4 2008 MG132 and ALLN inhibited the release of cytochrome c from mitochondria into the cytosol in the absence of survival factors and suppressed the cleavage of pro-caspase-9 and -3 to its active forms induced by etoposide. Etoposide 206-215 caspase 3 Homo sapiens 154-174 19349754-4 2009 This compound showed inhibitory activity on caspase-3 induction, a major protease of the apoptosis cascade, with an IC50 value of 38.96 microM after 8 h of etoposide treatment in U937 cells. Etoposide 156-165 caspase 3 Homo sapiens 44-53 19349754-5 2009 The expression level of caspase-3 and poly(ADP-ribose) polymerase (PARP) were dose-dependently inhibited by the compound, suggesting that rengyolone inhibits etoposide-induced apoptosis via downregulation of caspases. Etoposide 158-167 caspase 3 Homo sapiens 24-33 18191951-7 2008 IFNalpha also activated caspases-3, -8 and -9 protein kinases and PARP cleavage in response to etoposide in U2OS cells. Etoposide 95-104 caspase 3 Homo sapiens 24-45 18366759-8 2008 Cluster analysis of data unravels several possible pathways involved in the hypoxia-induced protection against etoposide-induced apoptosis: one of them could be the inhibition of p53 activity under hypoxia since caspase 3 activity parallels Bax and Bak expression profile. Etoposide 111-120 caspase 3 Homo sapiens 212-221 18319618-3 2008 HAX-1 was cleaved by CASP3 during etoposide-(ETO) induced apoptosis, and this event was inhibited by a CASP3-specific inhibitor. Etoposide 34-44 caspase 3 Homo sapiens 21-26 18319618-3 2008 HAX-1 was cleaved by CASP3 during etoposide-(ETO) induced apoptosis, and this event was inhibited by a CASP3-specific inhibitor. Etoposide 34-44 caspase 3 Homo sapiens 103-108 17765869-4 2007 BRI3BP overexpression enhanced both mitochondrial cytochrome c release and caspase-3 activity in etoposide-treated cells. Etoposide 97-106 caspase 3 Homo sapiens 75-84 18060501-7 2008 We demonstrate in vitro and in vivo that CSN6 is cleaved most effectively by caspase 3 at D23 after 2-3 h of apoptosis induced by anti-Fas-Ab or etoposide. Etoposide 145-154 caspase 3 Homo sapiens 77-86 17276981-6 2007 In support of an apoptosis rather than necrosis process, Aph-1 and Pen-2 also lower staurosporine- and etoposide-induced caspase-3 expression and diminished caspase-3 activity and poly(ADP-ribose) polymerase inactivation. Etoposide 103-112 caspase 3 Homo sapiens 121-130 17785460-6 2007 The induction of Bcl-2 inhibited apoptosis induced by serum starvation or etoposide, and PKCepsilon activation attenuated etoposide-induced caspase-3 cleavage. Etoposide 122-131 caspase 3 Homo sapiens 140-149 17827720-9 2007 These results indicate that Neu3 is up-regulated in Jurkat cells undergoing etoposide-induced apoptosis through intracellular signaling events downstream of caspase 3 activation and that enhanced Neu3 activity is closely related to the changes of cell surface ganglioside composition. Etoposide 76-85 caspase 3 Homo sapiens 157-166 17534580-10 2007 Finally, a significant increase in caspase-3 activity and apoptosis was observed in the presence of both VPA and etoposide compared to either agent alone. Etoposide 113-122 caspase 3 Homo sapiens 35-44 18219994-5 2007 The increase in MLD content was registered as early as 60 min after the addition of etoposide coinciding with the time course of caspase-3 activation. Etoposide 84-93 caspase 3 Homo sapiens 129-138 17297310-4 2007 Using multiparameter cytometry we measured the expression of ATM-S1981P and gammaH2AX as well as initiation of apoptosis (caspase-3 activation) in relation to the cell cycle phase in etoposide-treated human lymphoblastoid TK6 cells. Etoposide 183-192 caspase 3 Homo sapiens 122-131 17384250-0 2006 Alterations in mRNA expression of apoptosis-related genes BCL2, BAX, FAS, caspase-3, and the novel member BCL2L12 after treatment of human leukemic cell line HL60 with the antineoplastic agent etoposide. Etoposide 193-202 caspase 3 Homo sapiens 74-83 16715131-4 2006 In this study, both etoposide and excisanin A, two anticancer agents, induced apoptosis in colon cancer cell line SW620 as determined by Annexin V staining, the cleavage of caspase-3 and the proteolytic degradation of poly (ADP-ribose) polymerase (PARP). Etoposide 20-29 caspase 3 Homo sapiens 173-182 17217622-7 2006 Furthermore, endogenous levels of pro-caspase-3 were decreased by overexpression of SAG/ROC-SCF(beta-TrCP) E3 Ub ligases, but increased on siRNA silencing of SAG, regulator of cullin-1 (ROC1), or beta-TrCPs, leading to increased apoptosis by etoposide and TNF-related apoptosis-inducing ligand through increased activation of caspase-3. Etoposide 242-251 caspase 3 Homo sapiens 34-47 17217622-7 2006 Furthermore, endogenous levels of pro-caspase-3 were decreased by overexpression of SAG/ROC-SCF(beta-TrCP) E3 Ub ligases, but increased on siRNA silencing of SAG, regulator of cullin-1 (ROC1), or beta-TrCPs, leading to increased apoptosis by etoposide and TNF-related apoptosis-inducing ligand through increased activation of caspase-3. Etoposide 242-251 caspase 3 Homo sapiens 38-47 17013758-3 2006 We have previously shown that caspase-3 reconstitution significantly sensitized MCF-7 cells to doxorubicin and etoposide. Etoposide 111-120 caspase 3 Homo sapiens 30-39 16951922-5 2006 Etoposide-induced apoptosis in HL60 cells was significantly increased in ascorbate-loaded cells as measured by caspase-3 activation and DNA degradation, and this appeared to reflect a decrease in the number of necrotic cells rather than increased cytotoxicity. Etoposide 0-9 caspase 3 Homo sapiens 111-120 16801388-1 2006 We show that caspase-3 cleaves Cdc6 at D(290)/S and D(442)/G sites, producing p32-tCdc6 (truncated Cdc6) and p49-tCdc6, respectively, during etoposide- or tumor necrosis factor (TNF)-alpha-induced apoptosis. Etoposide 141-150 caspase 3 Homo sapiens 13-22 16598774-8 2006 Furthermore, siRNA suppression of HSP27 expression significantly decreased KNG inhibition of etoposide-induced caspase-3 activation and apoptosis in these cells. Etoposide 93-102 caspase 3 Homo sapiens 111-120 16575541-5 2006 Caspase activity assay showed that our peptide successfully increased the activity of caspase-3 and caspase-9 in etoposide-induced apoptosis. Etoposide 113-122 caspase 3 Homo sapiens 86-95 16331277-4 2006 Conversely, restoration of procaspase-3 sensitizes MCF-7 cells to chemotherapeutics including epirubicine, etoposide and taxol. Etoposide 107-116 caspase 3 Homo sapiens 27-39 16699130-2 2006 In this study, the authors describe a multiplexed assay for caspase 3 activation, nuclear condensation, and cell viability in a neuronal precursor cell line Ntera-2, injured with staurosporine and etoposide. Etoposide 197-206 caspase 3 Homo sapiens 60-69 16467399-5 2006 In JAR cells, etoposide increased expression of the proteins including IFNgammaR, p53 and pro-caspase 3 as well as IRF-1 mRNA and IFNgamma-pretreatment apparently promoted up-regulation of these molecules expression. Etoposide 14-23 caspase 3 Homo sapiens 90-103 16044155-12 2005 Thus, in these cell lines, Smac-mimic acts in an apparent IAPs dependent manner to induce apoptosis alone as well as sensitizes breast cancer cells to TRAIL or etoposide induced apoptosis via caspase-3 activation. Etoposide 160-169 caspase 3 Homo sapiens 192-201 15455353-3 2005 Using an inducible system of LMP1 expression in HeLa cells, we show herein that etoposide-triggered apoptosis, as evidenced by nuclear condensation and caspase-3 activation, is enhanced by LMP1. Etoposide 80-89 caspase 3 Homo sapiens 152-161 16010441-13 2005 We also found that etoposide bypassed Fas-FADD interaction in MML-1R by activating caspase-8 and caspase-3. Etoposide 19-28 caspase 3 Homo sapiens 97-106 15924153-6 2005 Since etoposide-induced apoptosis involved caspase 3 activation in Hank-1 and NK-YS cells, the caspase 3-dependent apoptotic machinery appears to be intact. Etoposide 6-15 caspase 3 Homo sapiens 43-52 16014620-4 2005 We were also able to determine that sustained ERK activation was intimately associated with the etoposide-induced apoptosis of HaCaT cells, and treatment with 3,4"-DHF induced a significant suppression of etoposide-induced ERK activation, concomitant with the repression of poly(ADP-ribose) polymerase or the cleavage of pro-caspase 3. Etoposide 205-214 caspase 3 Homo sapiens 321-334 15388581-4 2005 Ectopic expression of hsp70 in HL-60 cells (HL-60/hsp70) inhibited Ara-C and etoposide-induced Bax conformation change and translocation to the mitochondria; attenuated the accumulation of cytochrome c, Smac, and Omi/HtrA2 in the cytosol; and inhibited the processing and activity of caspase-9 and caspase-3. Etoposide 77-86 caspase 3 Homo sapiens 298-307 15454884-5 2004 Treatment of HUVECs with LLL-CHO, etoposide, or C2-ceramide induced DeltaPsim, activation of caspase-3, caspase-8, and caspase-9 and the appearance of hypodiploid DNA-positive cells. Etoposide 34-43 caspase 3 Homo sapiens 93-102 15829155-0 2005 Insulin-like growth factor-I decreased etoposide-induced apoptosis in glioma cells by increasing bcl-2 expression and decreasing CPP32 activity. Etoposide 39-48 caspase 3 Homo sapiens 129-134 15657350-9 2005 Additionally, (-)-gossypol was more efficient than etoposide at inducing caspase-3 activation and phosphatidylserine externalization in the setting of Bcl-2 or Bcl-X(L) overexpression. Etoposide 51-60 caspase 3 Homo sapiens 73-82 15829155-10 2005 Etoposide increased the expression of wild-type p53, activated CPP32 (but not ICE) activity, and induced apoptosis in these cells. Etoposide 0-9 caspase 3 Homo sapiens 63-68 15829155-11 2005 IGF-I prevented etoposide-induced apoptosis by increasing the expression of bcl-2 and decreasing the activity of CPP32. Etoposide 16-25 caspase 3 Homo sapiens 113-118 15829155-13 2005 CONCLUSIONS: IGF-I decreased etoposide-induced apoptosis in glioma cells by increasing the expression of bcl-2 and decreasing the activity of CPP32. Etoposide 29-38 caspase 3 Homo sapiens 142-147 15273737-3 2004 TNFalpha pretreatment resulted in enhanced cleavage and activity of caspase-3 upon addition of etoposide, epirubicin or ceramide. Etoposide 95-104 caspase 3 Homo sapiens 68-77 15087450-7 2004 In addition, we demonstrate that a caspase-3 cleavage-resistant D418E MLH1 mutant inhibits etoposide-induced apoptosis but has little effect on TRAIL-induced apoptosis. Etoposide 91-100 caspase 3 Homo sapiens 35-44 15370291-5 2004 Furthermore, a 24-h pretreatment of HUVECs by CsA at 10 microg/mL significantly protected the cells by preventing both bcl-2 level decrease and caspase-3 activation related to etoposide-induced apoptosis. Etoposide 176-185 caspase 3 Homo sapiens 144-153 14742324-7 2004 In response to etoposide (ET), RasGAP fragment N was further cleaved in direct dependence on caspase-3 activity, which was more pronounced in NSCLC cells. Etoposide 15-24 caspase 3 Homo sapiens 93-102 14742324-7 2004 In response to etoposide (ET), RasGAP fragment N was further cleaved in direct dependence on caspase-3 activity, which was more pronounced in NSCLC cells. Etoposide 26-28 caspase 3 Homo sapiens 93-102 15087450-5 2004 Furthermore, MLH1 is rapidly proteolyzed by caspase-3 in cancer cells induced to undergo apoptosis by treatment with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) or the topoisomerase II inhibitor etoposide, which damages DNA. Etoposide 215-224 caspase 3 Homo sapiens 44-53 15040005-7 2004 SHP-2(Ex3-/-) fibroblasts exhibited enhanced levels of etoposide-induced caspase 3 activity as compared to wild-type fibroblasts and the enhanced level of caspase 3 activity was suppressed by a caspase 3-specific inhibitor. Etoposide 55-64 caspase 3 Homo sapiens 73-82 15171819-0 2004 Caspase-3 is dually regulated by apoptogenic factors mitochondrial release and by SAPK/JNK metabolic pathway in leukemic cells exposed to etoposide-ionizing radiation combined treatment. Etoposide 138-147 caspase 3 Homo sapiens 0-9 15040005-8 2004 Re-introduction of wild-type SHP-2 into the SHP-2(Ex3-/-) fibroblasts rescued the hypersensitivity to etoposide-induced caspase 3 activation. Etoposide 102-111 caspase 3 Homo sapiens 120-129 12121973-8 2002 Treatment of NCI-H82 cells with etoposide induces a series of time-dependent events, including the release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP). Etoposide 32-41 caspase 3 Homo sapiens 189-198 14991905-8 2004 Treatment of U937 cells with etoposide rapidly induced cell death and allowed the detection of active caspase-3 with approximately 2500 cells (0.8 pg of protein). Etoposide 29-38 caspase 3 Homo sapiens 102-111 15015589-7 2004 In conclusion, actinomycin D and etoposide stimulated caspase-3 activation in a Ewing tumor cell line and in freshly isolated tumor cells, causing drug-induced cell death. Etoposide 33-42 caspase 3 Homo sapiens 54-63 12907245-4 2003 Vinblastine, doxorubicin, or etoposide (VP-16) induced apoptotic cell death in KB-3 carcinoma cells, with similar kinetic profiles of PARP cleavage, caspase 3 activation, and mitochondrial cytochrome c release. Etoposide 29-38 caspase 3 Homo sapiens 149-158 14745596-15 2004 Caspase-3 cleavage occurred in the EBV-negative etoposide-treated cells but not in the EBV-positive cells. Etoposide 48-57 caspase 3 Homo sapiens 0-9 14672900-7 2004 LLL-CHO, etoposide, and C2-ceramide also caused DeltaPsim, the increment of both hypodiploid DNA(+) cells and TUNEL(+) cells, and the activation of both Leu-Glu-His-Asp ase (LEHDase; caspase-9 like activity) and Asp-Glu-Val-Asp ase (DEVDase; caspase-3 like activity) in synovial cells. Etoposide 9-18 caspase 3 Homo sapiens 242-251 14517333-1 2003 We show that Cdc6, an essential initiation factor for DNA replication, undergoes caspase-3-mediated cleavage in the early stages of apoptosis in HeLa cells and SK-HEP-1 cells induced by etoposide, paclitaxel, ginsenoside Rh2, or tumor necrosis factor-related apoptosis-inducing ligand. Etoposide 186-195 caspase 3 Homo sapiens 81-90 12738983-7 2003 After genotoxic stress such as etoposide treatment, AS2 expressing cells readily progressed into apoptosis through p53 and caspase-3 activations. Etoposide 31-40 caspase 3 Homo sapiens 123-132 12700630-3 2003 Our results indicate that, while certain typical features of apoptosis induced by etoposide--namely classical morphological changes and the ability to degrade DNA into oligonucleosomal fragments - are caspase-3-dependent, loss of cell adhesion to plastic and the capacity to interact with, and to be phagocytosed by, human monocyte-derived macrophages - both by CD14-dependent and CD14-independent mechanisms--do not require caspase-3. Etoposide 82-91 caspase 3 Homo sapiens 201-210 12700630-3 2003 Our results indicate that, while certain typical features of apoptosis induced by etoposide--namely classical morphological changes and the ability to degrade DNA into oligonucleosomal fragments - are caspase-3-dependent, loss of cell adhesion to plastic and the capacity to interact with, and to be phagocytosed by, human monocyte-derived macrophages - both by CD14-dependent and CD14-independent mechanisms--do not require caspase-3. Etoposide 82-91 caspase 3 Homo sapiens 425-434 12700630-4 2003 Furthermore, both etoposide-induced caspase-3-positive and -negative MCF-7 cells suppressed proinflammatory cytokine release by macrophages. Etoposide 18-27 caspase 3 Homo sapiens 36-45 12417047-3 2002 We found that the overexpression of FADD sensitizes etoposide-induced apoptosis through a rapid activation of c-Jun NH(2)-terminal kinase (JNK) and, subsequently, of caspase 3. Etoposide 52-61 caspase 3 Homo sapiens 166-175 11306462-7 2001 Although etoposide-induced activation of caspase-3 was followed by DNA ladder formation in P39 cells, E-64d, an inhibitor of lysosomal thiol proteases, specifically suppressed etoposide-induced activation of caspase-3. Etoposide 9-18 caspase 3 Homo sapiens 41-50 12111698-10 2002 CTPE cells also exhibited lower caspase-3 activity vs. RWPE-1 after etoposide treatment. Etoposide 68-77 caspase 3 Homo sapiens 32-41 11694507-9 2002 Coexpression of caspase-3-resistant PARP-1 mutant with DNAS1L3 in osteosarcoma cells blocked etoposide-induced internucleosomal DNA fragmentation and resulted in persistent poly(ADP-ribosyl)ation of DNAS1L3; it did not, however, prevent the activation of caspase-3 and the consequent cleavage of endogenous PARP-1. Etoposide 93-102 caspase 3 Homo sapiens 16-25 11694507-9 2002 Coexpression of caspase-3-resistant PARP-1 mutant with DNAS1L3 in osteosarcoma cells blocked etoposide-induced internucleosomal DNA fragmentation and resulted in persistent poly(ADP-ribosyl)ation of DNAS1L3; it did not, however, prevent the activation of caspase-3 and the consequent cleavage of endogenous PARP-1. Etoposide 93-102 caspase 3 Homo sapiens 255-264 12587780-2 2002 Here we show that 10 microg/ml etoposide elicited only minor changes in Bowes human melanoma cells (temporary decrease in cell viability and proliferation, transient phospatidylserine externalization and caspase-3 activation), which weren"t clearly capable to start apoptotic pathway in the entire treated population. Etoposide 31-40 caspase 3 Homo sapiens 204-213 11297734-6 2001 Furthermore, etoposide exposure of stable DFNA5 transfectants resulted in an increase of caspase-3-mediated apoptotic events in DFNA5-transfected clones in comparison to MeWo ETO 1 cells and controls. Etoposide 13-22 caspase 3 Homo sapiens 89-98 11604558-5 2001 Etoposide rapidly induced apoptosis, dependent on caspase-3 and -8, but inhibition of these caspases did not prevent major cell death, but promoted a switch in late morphology. Etoposide 0-9 caspase 3 Homo sapiens 50-66 11306462-7 2001 Although etoposide-induced activation of caspase-3 was followed by DNA ladder formation in P39 cells, E-64d, an inhibitor of lysosomal thiol proteases, specifically suppressed etoposide-induced activation of caspase-3. Etoposide 9-18 caspase 3 Homo sapiens 208-217 11306462-7 2001 Although etoposide-induced activation of caspase-3 was followed by DNA ladder formation in P39 cells, E-64d, an inhibitor of lysosomal thiol proteases, specifically suppressed etoposide-induced activation of caspase-3. Etoposide 176-185 caspase 3 Homo sapiens 208-217 11306462-9 2001 These findings indicate that lysosomal dysfunction induced by a reduction in ATP results in leakage of lysosomal enzymes into the cytosolic compartment and that lysosomal enzyme(s) may be involved in activation of caspase-3 during apoptosis in P39 cells treated with etoposide. Etoposide 267-276 caspase 3 Homo sapiens 214-223 11196185-0 2001 Reconstitution of caspase 3 sensitizes MCF-7 breast cancer cells to doxorubicin- and etoposide-induced apoptosis. Etoposide 85-94 caspase 3 Homo sapiens 18-27 11306736-4 2001 When HL-60 cells, p53 null, were treated with etoposide, MDM2 was markedly decreased prior to caspase-3-dependent retinoblastoma tumor suppressor protein (pRb) and poly (ADP- ribose) polymerase (PARP) cleavages. Etoposide 46-55 caspase 3 Homo sapiens 94-103 11029754-7 2000 Pretreatment with a specific calpain inhibitor calpeptin inhibited etoposide-induced calpain activation, Bax cleavage, cytochrome c release, and caspase-3 activation. Etoposide 67-76 caspase 3 Homo sapiens 145-154 11154867-2 2001 It has been demonstrated that the protease caspase-3, a downstream molecule of the CD95 pathway, is activated in UV-exposed HaCaT cells, and that the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is cleaved by interleukin-1beta converting enzyme (ICE)-like protease during apoptosis induced by X-rays, staurosporine and etoposide. Etoposide 332-341 caspase 3 Homo sapiens 43-52 10839193-4 2000 Processing of caspase-3 was slightly detected from 12 h and became obvious at 24 h with 100 microM etoposide. Etoposide 99-108 caspase 3 Homo sapiens 14-23 10930537-5 2000 Pretreatment of U937 cells with MG132 or LLnL inhibited etoposide-induced morphological apoptosis and caspase-3 activation. Etoposide 56-65 caspase 3 Homo sapiens 102-111 11042671-3 2000 Etoposide induced increased formation of ceramide from sphingomyelin and release of mitochondrial cytochrome c followed by activation of caspase-9 and caspase-3, but not caspase-1. Etoposide 0-9 caspase 3 Homo sapiens 151-160 11042671-6 2000 Reduced glutathione or N-acetylcysteine, which could reduce ceramide formation by inhibiting sphingomyelinase activity, prevented C6 cells from etoposide-induced apoptosis through blockage of caspase-3 activation and change of the Bax/Bcl-2 ratio. Etoposide 144-153 caspase 3 Homo sapiens 192-201 10930016-0 2000 Wild-type p53-dependent etoposide-induced apoptosis mediated by caspase-3 activation in human glioma cells. Etoposide 24-33 caspase 3 Homo sapiens 64-73 10930016-7 2000 A CPP32 inhibition assay was used to determine whether a specific CPP32 inhibitor, DEVD-CHO, affects the apoptosis induced by etoposide in malignant glioma cells. Etoposide 126-135 caspase 3 Homo sapiens 66-71 10930016-9 2000 Treatment with low concentrations of etoposide resulted in the increased expression of wild-type p53; it also initiated CPP32 activity and induced apoptosis in the U-87MG cells. Etoposide 37-46 caspase 3 Homo sapiens 120-125 10930016-11 2000 Furthermore, low concentrations of etoposide also induced apoptosis in the T-98G/p53 cells by enhancing the expression of transfected wild-type p53, decreasing the expression of bcl-2, and activating CPP32 activity. Etoposide 35-44 caspase 3 Homo sapiens 200-205 10930016-13 2000 Etoposide-induced apoptosis can be suppressed by the CPP32 inhibitor DEVD-CHO. Etoposide 0-9 caspase 3 Homo sapiens 53-58 10930016-14 2000 CONCLUSIONS: These findings indicate that wild-type p53, CPP32, and bcl-2 may mediate apoptosis induced by etoposide. Etoposide 107-116 caspase 3 Homo sapiens 57-62 10839193-8 2000 The downregulation of Bcl-X(L) protein occurred from 6 h, while Bax protein conversely showed a slight increase from 6 h. Taken together, the present findings show that the dose-dependent apoptotic effect of etoposide is based on a change in the balance between Bcl-X(L) and Bax, which precedes the activation of caspase-3. Etoposide 208-217 caspase 3 Homo sapiens 313-322 10405175-0 1999 Imaging of caspase-3 activation in HeLa cells stimulated with etoposide using a novel fluorescent probe. Etoposide 62-71 caspase 3 Homo sapiens 11-20 10637446-3 1999 Overexpression of human caspase-3 alone could not induce apoptosis of tumor cell lines, but apoptosis was markedly enhanced by the addition of etoposide. Etoposide 143-152 caspase 3 Homo sapiens 24-33 10637446-4 1999 In an AH130 liver tumor model, transduction of human caspase- 3, but not the empty vector, induced extensive apoptosis and reduced tumor volume when combined with etoposide administration. Etoposide 163-172 caspase 3 Homo sapiens 53-63 10497210-2 1999 We report here that during apoptosis induced by the topoisomerase II inhibitor etoposide, a fraction of GRP94 associated with the endoplasmic reticulum membrane undergoes specific proteolytic cleavage, coinciding with the activation of the caspase CPP32 and initiation of DNA fragmentation. Etoposide 79-88 caspase 3 Homo sapiens 248-253 10454539-6 1999 In addition, A1 potently inhibited etoposide-induced apoptosis by inhibiting the release of cytochrome c and by blocking caspase 3 activation. Etoposide 35-44 caspase 3 Homo sapiens 121-130 10430629-6 1999 Programmed cell death induced by etoposide was specifically blocked by peptides inhibitory for the caspase-1 or caspase-3 subfamilies of caspases. Etoposide 33-42 caspase 3 Homo sapiens 112-121 10405175-4 1999 Using one of these probes, we succeeded in microscopic visualization of caspase-3-like activity within HeLa cells treated with etoposide. Etoposide 127-136 caspase 3 Homo sapiens 72-81 10050878-5 1999 Exposure of mock-transfected U937 cells to 50 microm etoposide activates procaspase-3 and the long isoform of procaspase-2 and induces mitochondrial potential decrease and cytochrome c release from mitochondria to the cytosol. Etoposide 53-62 caspase 3 Homo sapiens 73-85 10381626-2 1999 Exposure of undifferentiated U937 cells to 50 microM etoposide for 6 h, that triggers apoptosis in 80% cells, activates procaspase-2L, -3 and -8, induces the mitochondrial release of cytochrome c and decreases Mcl-1 expression without modifying Bcl-2, Bcl-xL and Bax protein levels. Etoposide 53-62 caspase 3 Homo sapiens 120-144 9671409-4 1998 Treatment of U937 and HL60 leukemic cells and HT29 colon carcinoma cells with the topoisomerase II inhibitor etoposide upregulates CASP-2 and CASP-3 genes in these cells before inducing their apoptosis. Etoposide 109-118 caspase 3 Homo sapiens 142-148 9850095-6 1998 MCL1 decreased the caspase 3 activity induced by exposure to etoposide and increased the viability of the transfected cells after etoposide exposure. Etoposide 61-70 caspase 3 Homo sapiens 19-28 9485009-3 1998 The carbamoylating decomposite, 2-chloroethyl-isocyanate (6-25 microM), also decreased ETO-induced apoptosis in HT58 cells in vitro and their caspase 3-like activity ex vivo, whereas N-(2-chloroethyl)-N-nitrosocarbamoyl-valinamide, an alkylating and mainly intramolecularly carbamoylating nitrosourea derivative (400 microM), did not influence these phenomena. Etoposide 87-90 caspase 3 Homo sapiens 142-151 9588194-4 1998 Etoposide-induced activation of intracellular DEVD-pNa cleaving activity and apoptosis was suppressed by low micromolar concentrations of cell-permeable inhibitors of caspase-3. Etoposide 0-9 caspase 3 Homo sapiens 167-176 9516141-7 1998 We conclude that the cytosolic pathway that leads to apoptotic DNA fragmentation in etoposide-treated leukemic cells is delayed upstream of procaspase-3-mediated events in bcr-abl+ cell lines. Etoposide 84-93 caspase 3 Homo sapiens 140-152 9533766-5 1998 2DG inhibited the etoposide-induced activation of c-Jun N-terminal kinase 1/stress-activated protein kinase (JNK1/SAPK) and the subsequent activation of CPP32, both of which are positive regulators for etoposide-induced apoptosis of U937 cells. Etoposide 18-27 caspase 3 Homo sapiens 153-158 9533766-5 1998 2DG inhibited the etoposide-induced activation of c-Jun N-terminal kinase 1/stress-activated protein kinase (JNK1/SAPK) and the subsequent activation of CPP32, both of which are positive regulators for etoposide-induced apoptosis of U937 cells. Etoposide 202-211 caspase 3 Homo sapiens 153-158 9242521-4 1997 Immunoblotting showed that treatment of wild-type Jurkat cells with anti-Fas or the topoisomerase II-directed agent etoposide resulted in proteolytic cleavage of precursors for the cysteine-dependent aspartate-directed proteases caspase-3 and caspase-7 and degradation of the caspase substrates poly(ADP-ribose) polymerase (PARP) and lamin B1. Etoposide 116-125 caspase 3 Homo sapiens 229-238 9144536-3 1997 In the present study, we searched a protein data base for potential death substrates possessing the CPP32 cleavage site, DEVD, and identified several candidates including RFC140, the large subunit of replication factor C, which we subsequently demonstrated to be specifically cleaved in a variety of cell types undergoing apoptosis in response to different cytotoxic agents, whereas no degradation is observed in a cell line resistant to etoposide-induced apoptosis. Etoposide 438-447 caspase 3 Homo sapiens 100-105 9070648-7 1997 Moreover, Benzyloxycarbonyl-Glu-Val-Asp-CH2OC(O)-2,6,-dichlorobenzene (Z-EVD-CH2-DCB), a selective inhibitor of CPP-32(-like) protease, efficiently inhibited the cleavage of actin and the apoptosis of VP-16-treated U937 cells. Etoposide 201-206 caspase 3 Homo sapiens 112-118 8840993-13 1996 These results suggest that the activation of the Yama protease and PARP degradation are involved in Ara-C-, etoposide-, or mitoxantrone-induced apoptosis. Etoposide 108-117 caspase 3 Homo sapiens 49-53 9054443-3 1997 Immunoblotting revealed that levels of procaspase-2 did not change during etoposide-induced apoptosis, whereas levels of procaspase-3 diminished markedly 2-3 h after etoposide addition. Etoposide 166-175 caspase 3 Homo sapiens 121-133 8663611-4 1996 In U937 cells, both PI 3-kinase inhibitors (wortmannin and LY294002) and etoposide activated the CPP32 apoptotic protease by cleavage to active p17 subunits. Etoposide 73-82 caspase 3 Homo sapiens 97-102