PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
2541470-6	1989	VIP binding activity in the CHAPS extract was destroyed by trypsin or dithiothreitol in accordance with the known sensitivity of membrane-bound receptors to these agents.	Dithiothreitol	70-84	vasoactive intestinal peptide	Rattus norvegicus	0-3	
1965036-4	1990	A broad autoradiographic band which demonstrated a high affinity for VIP was identified at Mr 56,000 (53,000 in the absence of the reducing agent dithiothreitol) for both the solubilized and nonsolubilized receptors.	Dithiothreitol	146-160	vasoactive intestinal peptide	Rattus norvegicus	69-72	
8008637-8	1994	Treatment of membranes with dithiothreitol, trypsin, and phospholipases A2 and C resulted in a loss of the ability of these membranes to bind VIP.	Dithiothreitol	28-42	vasoactive intestinal peptide	Rattus norvegicus	142-145	
2839079-7	1988	The first subtype of VIP receptors is highly responsive to guanyl nucleotides and is easily altered by dithiothreitol.	Dithiothreitol	103-117	vasoactive intestinal peptide	Rattus norvegicus	21-24	
2541761-4	1989	This radioactive material was dramatically reduced when 1 microM unlabeled VIP was present in the incubation medium or when membranes were pretreated with trypsin or dithiothreitol.	Dithiothreitol	166-180	vasoactive intestinal peptide	Rattus norvegicus	75-78	
6329286-6	1984	By contrast, stimulation of adenylate cyclase by low VIP concentrations was specifically altered when beta-mercaptoethanol was used during tissue homogeneization at 5 degrees C. In addition, both VIP and secretin responses were highly sensitive towards a preincubation of 10 min at 25 degrees C in the presence of dithiothreitol.	Dithiothreitol	314-328	vasoactive intestinal peptide	Rattus norvegicus	196-199	
6329286-8	1984	125I-VIP binding was, indeed, reduced after dithiothreitol preincubation, low concentrations of the thiol reagent decreasing the apparent number of high-affinity VIP receptors and higher dithiothreitol concentrations reducing the affinity of VIP receptors.	Dithiothreitol	44-58	vasoactive intestinal peptide	Rattus norvegicus	5-8	
6329286-8	1984	125I-VIP binding was, indeed, reduced after dithiothreitol preincubation, low concentrations of the thiol reagent decreasing the apparent number of high-affinity VIP receptors and higher dithiothreitol concentrations reducing the affinity of VIP receptors.	Dithiothreitol	187-201	vasoactive intestinal peptide	Rattus norvegicus	5-8