PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7505022-1 1993 Three different agents, dithiothreitol (DTT), Mn2+, and phorbol ester (TPA), were found to induce HL-60 cell adhesion to fibronectin through distinct mechanisms. Dithiothreitol 24-38 fibronectin 1 Homo sapiens 121-132 7534805-10 1995 Inclusion of dithiothreitol or 2-mercaptoethanol during binding resulted in a concentration-dependent inhibition of transglutaminase-fibronectin interactions (IC50 = 1.5 mM and 20 mM, respectively). Dithiothreitol 13-27 fibronectin 1 Homo sapiens 133-144 8056752-1 1994 Purified plasma fibronectin in Tris-buffered saline aggregated on incubation at 37 degrees C in the presence of dithiothreitol without the presence of cells. Dithiothreitol 112-126 fibronectin 1 Homo sapiens 16-27 8056752-6 1994 The multimerization was not influenced by Na+, Ca2+, or EDTA, while urea-treated fibronectin required a higher concentration of dithiothreitol for multimer formation. Dithiothreitol 128-142 fibronectin 1 Homo sapiens 81-92 8907176-7 1996 Proteolytic susceptibility of the fibronectin to thermolysin or m-calpain changed within 3 h of incubation with dithiothreitol at 37 degrees C in accordance with the conformational change detected by circular dichroism. Dithiothreitol 112-126 fibronectin 1 Homo sapiens 34-45 7505022-1 1993 Three different agents, dithiothreitol (DTT), Mn2+, and phorbol ester (TPA), were found to induce HL-60 cell adhesion to fibronectin through distinct mechanisms. Dithiothreitol 40-43 fibronectin 1 Homo sapiens 121-132 7505022-5 1993 DTT and Mn2+ were both found to enhance the binding of fibronectin to purified alpha 5 beta 1, which suggests that both agents can directly stimulate the integrin-ligand binding reaction. Dithiothreitol 0-3 fibronectin 1 Homo sapiens 55-66 7505022-10 1993 In contrast, DTT and Mn2+ seem to act primarily by directly influencing the alpha 5 beta 1-fibronectin binding reaction. Dithiothreitol 13-16 fibronectin 1 Homo sapiens 91-102 7505022-11 1993 These studies characterize in detail a regulatory system for studying leukocyte alpha 5 beta 1-fibronectin adhesion and identify DTT as a novel activator of alpha 5 beta 1-fibronectin binding. Dithiothreitol 129-132 fibronectin 1 Homo sapiens 172-183 7419608-4 1980 The inhibition was overcome when DTT-treated human fibroblasts were plated on cold insoluble globulin (plasma fibronectin)--coated dishes. Dithiothreitol 33-36 fibronectin 1 Homo sapiens 110-121 1970486-2 1990 The addition of fibronectin and/or the reducing reagent dithiothreitol (DTT) to the factor XIIIa coagulation mixture led to the formation of a weaker gel structure, while the rigidity of thrombin induced clots was not appreciably affected by the inclusion of the DTT but increased somewhat in the presence of fibronectin. Dithiothreitol 56-70 fibronectin 1 Homo sapiens 309-320 1970486-2 1990 The addition of fibronectin and/or the reducing reagent dithiothreitol (DTT) to the factor XIIIa coagulation mixture led to the formation of a weaker gel structure, while the rigidity of thrombin induced clots was not appreciably affected by the inclusion of the DTT but increased somewhat in the presence of fibronectin. Dithiothreitol 72-75 fibronectin 1 Homo sapiens 16-27 1970486-2 1990 The addition of fibronectin and/or the reducing reagent dithiothreitol (DTT) to the factor XIIIa coagulation mixture led to the formation of a weaker gel structure, while the rigidity of thrombin induced clots was not appreciably affected by the inclusion of the DTT but increased somewhat in the presence of fibronectin. Dithiothreitol 72-75 fibronectin 1 Homo sapiens 309-320 1970486-2 1990 The addition of fibronectin and/or the reducing reagent dithiothreitol (DTT) to the factor XIIIa coagulation mixture led to the formation of a weaker gel structure, while the rigidity of thrombin induced clots was not appreciably affected by the inclusion of the DTT but increased somewhat in the presence of fibronectin. Dithiothreitol 263-266 fibronectin 1 Homo sapiens 16-27 3190520-7 1988 Inclusion of DTT appeared to reduce the degradative effect of both ECV and STE towards the type I collagen and plasma fibronectin substrates. Dithiothreitol 13-16 fibronectin 1 Homo sapiens 118-129 1761059-1 1991 Human plasma fibronectin was denatured with 8 M urea and reduced with dithiothreitol. Dithiothreitol 70-84 fibronectin 1 Homo sapiens 13-24 4063070-4 1985 The susceptibility of inter-chain disulfide bonds to 10mM 1,4-dithiothreitol at pH 7.8 as quantitated by the rate of reductive cleavage of fibronectin into its subunits was found to be only 8-fold larger than that of the intra-chain bonds. Dithiothreitol 58-76 fibronectin 1 Homo sapiens 139-150 7419608-6 1980 Analysis of the fibronectin concentrations in the human fibroblasts following DTT treatment indicated that there was little decrease in the absolute level of activity as determined in a biological assay for BHK cells spreading on culture dishes. Dithiothreitol 78-81 fibronectin 1 Homo sapiens 16-27 7419608-7 1980 Analysis of the fibronectin distribution on the DTT-treated human fibroblasts by indirect immunofluorescence using a specific anti-CIG antiserum revealed that fibronectin was no longer deposited onto the culture dish surfaces. Dithiothreitol 48-51 fibronectin 1 Homo sapiens 16-27 7419608-7 1980 Analysis of the fibronectin distribution on the DTT-treated human fibroblasts by indirect immunofluorescence using a specific anti-CIG antiserum revealed that fibronectin was no longer deposited onto the culture dish surfaces. Dithiothreitol 48-51 fibronectin 1 Homo sapiens 159-170 7419608-8 1980 Even when the DTT-treated human fibroblasts spread in the presence of fetal calf serum, the cell fibronectin remained for the most part in a perinuclear location. Dithiothreitol 14-17 fibronectin 1 Homo sapiens 97-108 7419608-9 1980 These results indicate that DTT treatment of human fibroblasts prevents the normal translocation of fibronectin from a perinulear location to the surface of the culture dish. Dithiothreitol 28-31 fibronectin 1 Homo sapiens 100-111 548650-8 1979 Spermidine also failed to inhibit cross-linking between fibronectin molecules when aggregation of fibronectin was induced with dithiothreitol. Dithiothreitol 127-141 fibronectin 1 Homo sapiens 98-109 667030-1 1978 Reduction of disulfide linkages by dithiothreitol removes LETS (large, external, transformation-sensitive) protein from the cell surface. Dithiothreitol 35-49 fibronectin 1 Homo sapiens 58-62 667030-3 1978 At 0 degrees C the release of LETS protein by dithiothreitol is completely blocked, but this is apparently not due to a requirement for metabolic energy. Dithiothreitol 46-60 fibronectin 1 Homo sapiens 30-34