PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 14654376-10 2003 The inhibition of the H(2)O(2)-induced activation of caspase-3, but not that of caspase-9, by nicorandil in the presence of 5-HD or by SNAP was reversed by the addition of dithiothreitol to the enzyme assay. Dithiothreitol 172-186 caspase 3 Homo sapiens 53-62 11023998-5 2000 In addition to inhibiting caspase-3 through a dithiothreitol-sensitive S-nitrosylation process, preconditioning stress concomitantly up-regulated the expression of the anti-apoptotic bcl-2 protein and down-regulated the p66shc adaptor protein. Dithiothreitol 46-60 caspase 3 Homo sapiens 26-35 12566444-8 2003 Moreover, S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine directly blocked the activity of recombinant caspase-3, which was reversed by the reducing agent dithiothreitol, whereas PAPA or DEA NONOate did not block the enzymatic activity of caspase-3. Dithiothreitol 164-178 caspase 3 Homo sapiens 112-121 12566444-8 2003 Moreover, S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine directly blocked the activity of recombinant caspase-3, which was reversed by the reducing agent dithiothreitol, whereas PAPA or DEA NONOate did not block the enzymatic activity of caspase-3. Dithiothreitol 164-178 caspase 3 Homo sapiens 248-257 11279547-0 2000 Apoptosis induced by dithiothreitol in HL-60 cells shows early activation of caspase 3 and is independent of mitochondria. Dithiothreitol 21-35 caspase 3 Homo sapiens 77-86 11279547-5 2000 The first caspase activity detectable in DTT-treated cells is caspase 3, which is increased significantly 1 - 2 h after the start of DTT treatment. Dithiothreitol 41-44 caspase 3 Homo sapiens 62-71 11279547-5 2000 The first caspase activity detectable in DTT-treated cells is caspase 3, which is increased significantly 1 - 2 h after the start of DTT treatment. Dithiothreitol 133-136 caspase 3 Homo sapiens 62-71 11279547-8 2000 Although oxidizing DTT can produce H2O2, data presented here indicate that DTT-induced apoptosis is not mediated by production of H2O2 and occurs via a novel pathway that involves activation of caspase 3 at early stages, prior to activation of the common "initiator" caspases 2, 8 and 9. Dithiothreitol 75-78 caspase 3 Homo sapiens 194-203 10722685-6 2000 The inhibitory action of selenite on a recombinant active caspase-3 could be reversed by sulfhydryl reducing agents, such as dithiothreitol and beta-mercaptoethanol. Dithiothreitol 125-139 caspase 3 Homo sapiens 58-67 10652216-5 2000 The EC(50) for caspase-3 activation by reduced thioredoxin-1 was 2.5 microM, by reduced glutathione 1.0 mM and by dithiothreitol 3.5 mM. Dithiothreitol 114-128 caspase 3 Homo sapiens 15-24 9441900-12 1997 The inhibition of caspase-3 activity correlated with a S-nitrosylation of the reactive cysteine residue and was reversed by further addition of dithiothreitol. Dithiothreitol 144-158 caspase 3 Homo sapiens 18-27 9862698-7 1998 Caspase-3 activity in cell lysate precultured with anti-Fas Ab was suppressed dose dependently by diamide and restored by thiol-reducing agents, DTT or TRX. Dithiothreitol 145-148 caspase 3 Homo sapiens 0-9 9706149-9 1998 Dithiothreitol partially reversed the NO-induced suppression of caspase-3-like activity, which is consistent with S-nitrosylation of caspase-3. Dithiothreitol 0-14 caspase 3 Homo sapiens 64-73 9706149-9 1998 Dithiothreitol partially reversed the NO-induced suppression of caspase-3-like activity, which is consistent with S-nitrosylation of caspase-3. Dithiothreitol 0-14 caspase 3 Homo sapiens 133-142 9388267-6 1997 Dithiothreitol (DTT), but not glutathione, reversed the inhibition of recombinant caspase-3 by NO. Dithiothreitol 0-14 caspase 3 Homo sapiens 82-91 9388267-6 1997 Dithiothreitol (DTT), but not glutathione, reversed the inhibition of recombinant caspase-3 by NO. Dithiothreitol 16-19 caspase 3 Homo sapiens 82-91 35571249-7 2022 Mechanistically, we found that H2S persulfidated caspase-3 in H9C2 cells and human recombinant caspase-3 protein, while the thiol-reducing agent dithiothreitol (DTT) abolished H2S-induced persulfidation of caspase-3 and thereby prevented the antiapoptotic effect of H2S on caspase-3 in H9C2 cells. Dithiothreitol 145-159 caspase 3 Homo sapiens 95-104 35571249-7 2022 Mechanistically, we found that H2S persulfidated caspase-3 in H9C2 cells and human recombinant caspase-3 protein, while the thiol-reducing agent dithiothreitol (DTT) abolished H2S-induced persulfidation of caspase-3 and thereby prevented the antiapoptotic effect of H2S on caspase-3 in H9C2 cells. Dithiothreitol 145-159 caspase 3 Homo sapiens 206-215 35571249-7 2022 Mechanistically, we found that H2S persulfidated caspase-3 in H9C2 cells and human recombinant caspase-3 protein, while the thiol-reducing agent dithiothreitol (DTT) abolished H2S-induced persulfidation of caspase-3 and thereby prevented the antiapoptotic effect of H2S on caspase-3 in H9C2 cells. Dithiothreitol 145-159 caspase 3 Homo sapiens 273-282 35571249-7 2022 Mechanistically, we found that H2S persulfidated caspase-3 in H9C2 cells and human recombinant caspase-3 protein, while the thiol-reducing agent dithiothreitol (DTT) abolished H2S-induced persulfidation of caspase-3 and thereby prevented the antiapoptotic effect of H2S on caspase-3 in H9C2 cells. Dithiothreitol 161-164 caspase 3 Homo sapiens 206-215 35571249-7 2022 Mechanistically, we found that H2S persulfidated caspase-3 in H9C2 cells and human recombinant caspase-3 protein, while the thiol-reducing agent dithiothreitol (DTT) abolished H2S-induced persulfidation of caspase-3 and thereby prevented the antiapoptotic effect of H2S on caspase-3 in H9C2 cells. Dithiothreitol 161-164 caspase 3 Homo sapiens 273-282 18768468-3 2008 Kinetic analysis indicates the compounds can irreversibly inactivate caspase-3 in a 1,4-dithiothreitol (DTT)- and oxygen-dependent manner, implying that a redox cycle might take place in the inactivation process. Dithiothreitol 84-102 caspase 3 Homo sapiens 69-78 18768468-3 2008 Kinetic analysis indicates the compounds can irreversibly inactivate caspase-3 in a 1,4-dithiothreitol (DTT)- and oxygen-dependent manner, implying that a redox cycle might take place in the inactivation process. Dithiothreitol 104-107 caspase 3 Homo sapiens 69-78 18768468-8 2008 Isoquinoline-1,3,4-trione derivative-catalyzed caspase-3 inactivation could also be observed when DTT is substituted with dihydrolipoic acid, which exists widely in cells and might play an important role in the in vivo inactivation process in which the inhibitors inactivate caspase-3 in cells and then prevent the cells from apoptosis. Dithiothreitol 98-101 caspase 3 Homo sapiens 47-56 18768468-8 2008 Isoquinoline-1,3,4-trione derivative-catalyzed caspase-3 inactivation could also be observed when DTT is substituted with dihydrolipoic acid, which exists widely in cells and might play an important role in the in vivo inactivation process in which the inhibitors inactivate caspase-3 in cells and then prevent the cells from apoptosis. Dithiothreitol 98-101 caspase 3 Homo sapiens 275-284 17093075-6 2006 In an ex vivo system, PN inhibited the activity of caspase-3, and this inhibition was reversible with the addition of the sulfhydryl reducing agent dithiothreitol, indicating that PN inhibits caspases by cysteinyl oxidation. Dithiothreitol 148-162 caspase 3 Homo sapiens 51-60 17093075-10 2006 Caspase-3 activity was inhibited in brain lysates harvested after FPI and was restored by adding dithiothreitol. Dithiothreitol 97-111 caspase 3 Homo sapiens 0-9 16844966-3 2006 While screening a library of small molecules against caspase-3, the authors have found that the nature of the reducing agent used, DTT or beta-MCE, dramatically affects screening results and leads to identification of nonoverlapping hits. Dithiothreitol 131-134 caspase 3 Homo sapiens 53-62 16620791-4 2006 Using the activation of caspase-3 as indicator of apoptosis, we found that in two cell lines, Jurkat and Mono-Mac 6, staurosporine and DTT elicited apoptosis with a different pattern: staurosporine acted rapidly and at nanomolar concentrations while DTT acted slowly and at higher concentrations (1mM). Dithiothreitol 135-138 caspase 3 Homo sapiens 24-33 16640590-3 2006 We show here that viable parasites prominently inhibited the activation of caspase 3/7 induced by cytochrome c, dATP and dithiothreitol in cytosolic extracts of human-derived Jurkat leukemic T cells. Dithiothreitol 121-135 caspase 3 Homo sapiens 75-84 9208169-12 1997 Significantly, dithiothreitol reduction of the DC disulfide abolished its inhibition of in vitro proenzyme processing, thereby demonstrating thiol-disulfide exchange between the DC disulfide and a free thiol group on an activator(s) of caspase-3. Dithiothreitol 15-29 caspase 3 Homo sapiens 236-245 17351060-6 2007 Consistent with this, CSE inhibited active recombinant caspase-3 activity, which was abolished by dithiothreitol, suggesting a redox-sensitive mechanism. Dithiothreitol 98-112 caspase 3 Homo sapiens 55-64