PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9199164-3 1997 In yeast cells grown on either ammonia or urea medium, the general amino acid permease (Gap1p) is transported from the Golgi complex to the plasma membrane, whereas, in cells grown on glutamate medium, Gap1p is transported from the Golgi to the vacuole. Ammonia 31-38 amino acid permease GAP1 Saccharomyces cerevisiae S288C 88-93 9611819-5 1998 Following an ammonia pulse, the expression of GAP1, PUT4 and GDH1 decreased while the intracellular glutamine concentration remained constant, both in the cytoplasm and in the vacuole. Ammonia 13-20 amino acid permease GAP1 Saccharomyces cerevisiae S288C 46-50 9199164-3 1997 In yeast cells grown on either ammonia or urea medium, the general amino acid permease (Gap1p) is transported from the Golgi complex to the plasma membrane, whereas, in cells grown on glutamate medium, Gap1p is transported from the Golgi to the vacuole. Ammonia 31-38 amino acid permease GAP1 Saccharomyces cerevisiae S288C 202-207 9199164-5 1997 In sec13 mutants grown on ammonia, Gap1p is transported from the Golgi to the vacuole, instead of to the plasma membrane. Ammonia 26-33 amino acid permease GAP1 Saccharomyces cerevisiae S288C 35-40 8497191-2 1993 GAP1 activity is regulated by control of synthesis and control of activity in response to the nitrogen source supplied; ammonia and glutamine inactivate GAP1 function while proline and urea allow its maximum expression. Ammonia 120-127 amino acid permease GAP1 Saccharomyces cerevisiae S288C 0-4 7592450-3 1995 Increased extracellular ammonia concentrations are correlated with increased intracellular glutamate and glutamine concentrations, increases in levels of NAD-dependent glutamate dehydrogenase activity and its mRNA (gene GDH2), and decreases in levels of NADPH-dependent glutamate dehydrogenase activity and its mRNA (gene GDH1), as well as decreases in the levels of mRNA for the amino acid permease-encoding genes GAP1 and PUT4. Ammonia 24-31 amino acid permease GAP1 Saccharomyces cerevisiae S288C 415-419 9179853-9 1997 In contrast, deletion of the last eleven amino acids of Gap1p, a region conserved in other amino acid permeases, conferred resistance to NH4+ inactivation. Ammonia 137-140 amino acid permease GAP1 Saccharomyces cerevisiae S288C 56-61 7773405-7 1995 It is shown that growth on ammonia alone is not sufficient to cause repression of GAP1 and GLN1 transcription and that the regulation of GAP1 transcription and both NADPH-GDH and GS activity is not an on/off switch, but is gradually modulated in correlation with the ammonia concentration. Ammonia 267-274 amino acid permease GAP1 Saccharomyces cerevisiae S288C 137-141 8497191-2 1993 GAP1 activity is regulated by control of synthesis and control of activity in response to the nitrogen source supplied; ammonia and glutamine inactivate GAP1 function while proline and urea allow its maximum expression. Ammonia 120-127 amino acid permease GAP1 Saccharomyces cerevisiae S288C 153-157 8497191-3 1993 We have isolated and characterized a gene, AUA1, involved in ammonia regulation of GAP1 activity. Ammonia 61-68 amino acid permease GAP1 Saccharomyces cerevisiae S288C 83-87 8497191-4 1993 AUA1 is not essential for growth but overexpression of the AUA1 transcript in a high-copy vector or due to a regulatory mutation, aua1-1, present approximately 10 bp upstream from the start of AUA1 transcription, releases GAP1 activity from ammonia-inactivation without affecting GAP1 transcription. Ammonia 241-248 amino acid permease GAP1 Saccharomyces cerevisiae S288C 222-226 6759873-7 1982 Mutations at three unlinked loci, namely MUT2, MUT4, and PGR, release one of the two controls which prevent expression of the GAP1 gene in ammonia-grown yeast cells. Ammonia 139-146 amino acid permease GAP1 Saccharomyces cerevisiae S288C 126-130 6343842-6 1983 In strains having one class of mutations (per1), ammonia inactivation of the PUT4 and GAP1 permeases did not occur, whereas glutamate and glutamine inactivation did. Ammonia 49-56 amino acid permease GAP1 Saccharomyces cerevisiae S288C 86-90 12074570-2 2002 Colonies of Saccharomyces cerevisiae mutant defective in the general amino acid permease, Gap1p, exhibit decreased ammonia production. Ammonia 115-122 amino acid permease GAP1 Saccharomyces cerevisiae S288C 90-95 17919965-4 2008 We have shown that Gap1p activity is lowered in cells with an activated RAS2(val19) allele or elevated cAMP levels whereas cells with inactive ras2 allele lose ammonia repression of Gap1p-mediated transport. Ammonia 160-167 amino acid permease GAP1 Saccharomyces cerevisiae S288C 182-187