PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
17461933-6	2007	RESULTS: Western blot analysis of immunoprecipitates showed that introduction of the inhibitory peptide decreased tyrosine phosphorylation of PMCA by nearly 60% in saponin-permeabilized, thrombin-treated platelets as compared with the scrambled control peptide.	Saponins	164-171	coagulation factor II, thrombin	Homo sapiens	187-195	
23499166-0	2013	Spirostanol saponins and esculin from Rusci rhizoma reduce the thrombin-induced hyperpermeability of endothelial cells.	Saponins	12-20	coagulation factor II, thrombin	Homo sapiens	63-71	
23499166-2	2013	To determine the effect of its secondary plant metabolites on the endothelium, phenolic compounds and saponins from Butcher"s broom were isolated from a methanolic extract, and their activity on the thrombin-induced hyperpermeability of human microvascular endothelial cells (HMEC-1) was investigated in vitro.	Saponins	102-110	coagulation factor II, thrombin	Homo sapiens	199-207	
23499166-7	2013	The highest activities were observed for the spirostanol saponins 5 and 8 and for esculin (4) at 10muM, and these compounds resulted in a reduction of the thrombin-induced hyperpermeability to 41.9%, 42.6% and 53.3%, respectively.	Saponins	57-65	coagulation factor II, thrombin	Homo sapiens	155-163	
8670130-5	1996	In washed, saponin-permeabilized platelets incubated with [gamma-32P]ATP, thrombin- and phorbol 12-myristate 13-acetate (PMA)-induced phosphorylation of several proteins (66, 45, and 20kDa) was inhibited by preincubation with AS peptide (KNVVGARRSSWRVISSIEQK) based on the pseudosubstrate-like region of the 14-3-3 family.	Saponins	11-18	coagulation factor II, thrombin	Homo sapiens	74-82	
1522120-7	1992	To examine further the signal transduction pathway initiated by thrombin, we developed novel conditions for minimal permeabilization of EC with saponin (4-8 micrograms/ml for 5-15 min at 37 degrees C) which allow the introduction of small extracellular molecules without the loss of large intracellular proteins and which retain thrombin-stimulated secretion.	Saponins	144-151	coagulation factor II, thrombin	Homo sapiens	64-72	
8824741-1	1995	The non-saponin fraction (NSF; lipophilic fraction) from the roots of Panax ginseng inhibited the aggregation of human platelets induced by thrombin (0.1 units/ml) in a dose-dependent manner.	Saponins	8-15	coagulation factor II, thrombin	Homo sapiens	140-148	
1935970-3	1991	Stimulation of intact platelets with the Ca(2+)-ionophore A23187 or thrombin, but not phorbol 12,13-dibutyrate, decreased the subsequent pertussis-toxin-dependent ADP ribosylation of Gi alpha in saponin-permeabilized platelets in a time-dependent and dose-dependent manner.	Saponins	195-202	coagulation factor II, thrombin	Homo sapiens	68-76	
2987242-0	1985	Thrombin induces serotonin secretion and aggregation independently of inositol phospholipids hydrolysis and protein phosphorylation in human platelets permeabilized with saponin.	Saponins	170-177	coagulation factor II, thrombin	Homo sapiens	0-8	
2829859-4	1988	Thrombin also stimulated 45Ca2+ uptake into saponin-treated platelets.	Saponins	44-51	coagulation factor II, thrombin	Homo sapiens	0-8	
3120720-1	1987	Human platelets labeled with [3H]arachidonic acid and permeabilized with saponin produced [3H]1,2-diacylglycerol (DG) by phospholipase C and released [3H]arachidonate by phospholipase A2, when activated with thrombin.	Saponins	73-80	coagulation factor II, thrombin	Homo sapiens	208-216	
3107563-1	1987	Guanosine 5"-O-thiotriphosphate (GTP gamma S) and thrombin stimulate the activity of phospholipase C in platelets that have been permeabilized with saponin and whose inositol phospholipids have been prelabeled with [3H]inositol.	Saponins	148-155	coagulation factor II, thrombin	Homo sapiens	50-58	
3023367-5	1986	In platelets permeabilized with saponin, nonhydrolyzable GTP analogs reproduced the effects of thrombin by causing diacylglycerol formation, Ca2+ release from the dense tubular system and serotonin secretion.	Saponins	32-39	coagulation factor II, thrombin	Homo sapiens	95-103	
3063257-0	1988	Action of guanosine 5"-[beta-thio]diphosphate on thrombin-induced activation and Ca2+ mobilization in saponin-permeabilized and intact human platelets.	Saponins	102-109	coagulation factor II, thrombin	Homo sapiens	49-57	
3023367-8	1986	Guanosine 5"-O-(2-thiodiphosphate) (GDP beta S), which inhibits G protein function, inhibited the ability of thrombin to cause IP3 and diacylglycerol formation, granule secretion, and Ca2+ release from the dense tubular system in saponin-treated platelets.	Saponins	230-237	coagulation factor II, thrombin	Homo sapiens	109-117	
3023367-11	1986	Thrombin-induced diacylglycerol formation and 45Ca release were also inhibited when the saponin-treated platelets were preincubated with pertussis toxin, an event that was associated with the ADP-ribosylation of a protein with Mr = 41.7 kDa.	Saponins	88-95	coagulation factor II, thrombin	Homo sapiens	0-8	
2939827-7	1986	Saponin-permeabilized platelets show identical platelet responses (shape change, aggregation and release of 5-hydroxy[14C]tryptamine) to both collagen (5 micrograms/ml) and thrombin (0.1 unit/ml) as obtained with intact cells, indicating that there is minimal disturbance to the surface membrane receptor topography for these two agonists.	Saponins	0-7	coagulation factor II, thrombin	Homo sapiens	173-181	
2939827-8	1986	Ins(1,4,5)P3 (1-10 microM) added to saponin-treated platelets (but not to intact platelets) induced dose-related shape change, aggregation and release of 5-hydroxy[14C]tryptamine which at maximal doses was comparable with responses obtained with thrombin or collagen.	Saponins	36-43	coagulation factor II, thrombin	Homo sapiens	246-254	
6229546-6	1984	The presence of fibrinogen, beta TG, and PF4 in corresponding large intracellular vacuoles and along the platelet plasma membrane after thrombin stimulation was demonstrated by immunocytochemical techniques in saponin-permeabilized and nonpermeabilized platelets.	Saponins	210-217	coagulation factor II, thrombin	Homo sapiens	136-144