PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30838000-8 2019 Upon in vitro activation with PMA plus ionomycin or IL12 plus IL18, fewer MAIT cells isolated from the young adult group expressed IFN-gamma, IL17A and Granzyme B then cells from other age groups while the proportion of cells that expressed TNF-alpha was similar. Ionomycin 39-48 tumor necrosis factor Homo sapiens 241-250 31295519-10 2019 Thapsigargin or ionomycin also phosphorylate p38 MAPK by Ca2+ influx through SOCE, leading to suppression of TNF-alpha-induced NF-kappaB phosphorylation. Ionomycin 16-25 tumor necrosis factor Homo sapiens 109-118 30998783-5 2019 As for effector functions, IFNgamma/TNF responses to phosphoantigens or PMA/Ionomycin in Vdelta2+gammadeltaT-cells were weaker in AHB but preserved in CHB, without significant differences for Vdelta1+gammadeltaT-cells. Ionomycin 76-85 tumor necrosis factor Homo sapiens 36-39 30998783-6 2019 Furthermore, early IFNgamma/TNF responses in Vdelta2+ gammadeltaT-cells to brief PMA/Ionomycin stimulation correlated inversely with serum ALT but not HBV DNA. Ionomycin 85-94 tumor necrosis factor Homo sapiens 28-31 27327083-5 2016 We also found a significant response of TBX15 to TNF-alpha activation of the NF-kappaB pathway using five cancer cell lines, and similar results were obtained when NF-kappaB was activated with PMA/ionomycin. Ionomycin 197-206 tumor necrosis factor Homo sapiens 49-58 30049932-6 2018 Also, intracellular Ca2+ increase, due to ionomycin treatment, induced hemichannel opening, but activated astrocyte migration only partially, and this limitation was overcome by pre-treatment with tumor necrosis factor (TNF) and Thy-1. Ionomycin 42-51 tumor necrosis factor Homo sapiens 197-218 30049932-6 2018 Also, intracellular Ca2+ increase, due to ionomycin treatment, induced hemichannel opening, but activated astrocyte migration only partially, and this limitation was overcome by pre-treatment with tumor necrosis factor (TNF) and Thy-1. Ionomycin 42-51 tumor necrosis factor Homo sapiens 220-223 28412245-7 2017 However, after PMA + ionomycin stimulation, the degranulation and TNFalpha expression by CXCR5+CD8+ T cells were significantly elevated to a level comparable with CXCR5-CD8+ T cells, whereas the IFNgamma expression by PMA + ionomycin-stimulated CXCR5+CD8+ T cells were significantly higher than that by CXCR5-CD8+ T cells. Ionomycin 21-30 tumor necrosis factor Homo sapiens 66-74 25862606-7 2015 TNFalpha- or PMA/Ionomycin-induced phosphorylation of p65 at serine 529 in cell line models and healthy donor lymphocytes served as the experimental model. Ionomycin 17-26 tumor necrosis factor Homo sapiens 0-16 24611083-6 2014 The frequency of IFN-gamma+/tumor necrosis factor-alpha (TNF-alpha)+CD45RO+CD4+ T-cells upon stimulation with phorbol myristate acetate (PMA)/Ionomycin was higher in 2-3 months old infants who received BCG vaccination at birth compared to those who did not. Ionomycin 142-151 tumor necrosis factor Homo sapiens 57-66 25559824-11 2015 Finally, PMA + ionomycin stimulation did not induce significant alterations on MSCs phenotype but did increase indoleamine-2,3-dioxygenase (IDO), inducible costimulatory ligand (ICOSL), IL-1beta, IL-8, and TNF-alpha mRNA expression. Ionomycin 15-24 tumor necrosis factor Homo sapiens 206-215 24706270-8 2014 By stimulation of PBMCs with PMA/ionomycin for 6 h, more than 1-2 % of total CD8 T cells are identified as positive in terms of multifunctionality, thus producing multiple cytokines--IL-2, TNFalpha, and IFNgamma--at single-cell level in case of all healthy donors. Ionomycin 33-42 tumor necrosis factor Homo sapiens 189-197 23021516-8 2013 However, AA increased PGD2 and TNF-alpha secretion by ionomycin/phorbol 12-myristate 13-acetate-stimulated HMC-1, whereas EPA and DHA more prominently inhibited IL-4 and IL-13 secretion. Ionomycin 54-63 tumor necrosis factor Homo sapiens 31-40 22415432-6 2012 After stimulation with PMA and ionomycin, gammadelta T cells from HBV-ACLF patients produced the greatest amount of TNF-alpha or IL-17 among the three groups. Ionomycin 31-40 tumor necrosis factor Homo sapiens 116-125 22355730-3 2012 PMA/ionomycin stimulated IFN-gamma+IL-2+TNF-alpha+ CD4 T cell responses were decreased in patients with smear-positive tuberculosis compared to those with smear-negative tuberculosis. Ionomycin 4-13 tumor necrosis factor Homo sapiens 40-49 22337239-1 2012 OBJECTIVE: Previous reports have shown an increase in peripheral blood mononuclear cells" (PBMC) Th17 cell subpopulation and tumor necrosis factor-alpha (TNF-alpha) secretion after in vitro stimulation with anti-CD3/CD28 or phorbol myristate acetate/ionomycin in ankylosing spondylitis (AS). Ionomycin 250-259 tumor necrosis factor Homo sapiens 154-163 22690041-6 2012 The percentage of CD4(+) cells producing interferon gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha) in response to PMA/ionomycin was significantly reduced in pregnancy. Ionomycin 133-142 tumor necrosis factor Homo sapiens 74-101 22690041-6 2012 The percentage of CD4(+) cells producing interferon gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha) in response to PMA/ionomycin was significantly reduced in pregnancy. Ionomycin 133-142 tumor necrosis factor Homo sapiens 103-112 22355730-5 2012 Therefore, our findings argue against the notion that Mycobacterium tuberculosis antigen-specific multifunctional Th1 responses in peripheral blood can serve as correlates of protective immunity against tuberculosis; they suggest that the decrease in PMA/ionomycin stimulated IFN-gamma+IL-2+TNF-alpha+ CD4 T cells may be applied for clinical diagnosis of active tuberculosis. Ionomycin 255-264 tumor necrosis factor Homo sapiens 291-300 18833003-6 2008 Detection of intracellular cytokine production (interleukin-2, interferon-gamma, and tumor necrosis factor-alpha) upon phorbol 12-myristate 13-acetate-ionomycin stimulation in CD8alphaalpha+ and CD8alphabeta+ T-cells revealed that CD8alphaalpha+ T-cells show a unique cytokine production pattern (tumor necrosis factor-alpha and interferon-gamma production) as compared with CD8alphabeta+ T-cells. Ionomycin 151-160 tumor necrosis factor Homo sapiens 85-112 19920149-4 2010 The physical association between PDK1 and ASK1 is mediated through the pleckstrin homology domain of PDK1 and the C-terminal regulatory domain of ASK1 and is decreased by ASK1-activating stimuli, such as H(2)O(2), tumor necrosis factor alpha, thapsigargin, and ionomycin, as well as insulin, a PDK1 stimulator. Ionomycin 261-270 tumor necrosis factor Homo sapiens 214-241 19769987-5 2009 Although several tumor necrosis factor-alpha- and phorbol-12-myristate-13-acetate/ionomycin-induced NF-kappaB target genes are CARM1 dependent, CARM1 enzymatic activity was dispensable for gene expression. Ionomycin 82-91 tumor necrosis factor Homo sapiens 17-44 18755820-1 2009 Intracellular production of TNFalpha and IL-2 after stimulation with phorbol myristate/ionomycin was flowcytometrically measured in CD4(+) T cells from peripheral blood (PB) and cerebrospinal fluid (CSF) of 29 patients with multiple sclerosis (MS), and 16 with other inflammatory and 41 with other non-inflammatory neurological diseases. Ionomycin 87-96 tumor necrosis factor Homo sapiens 28-36 18637422-7 2008 RESULTS: Negative correlation was found between expression of IFN-gamma and TNF-alpha after PMA/ionomycin stimulation and allergen stimulation (p > 0,05). Ionomycin 96-105 tumor necrosis factor Homo sapiens 76-85 16780501-3 2006 METHODS: Using peripheral blood mononuclear cells derived from individuals with severe (n=12) and mild atopic dermatitis (n=10) and from nonatopic controls (n=10), we investigated production by CD4+ T cells of tumour necrosis factor (TNF)-alpha, IL-4, IL-5, IL-13 and IL-10 in response to phorbol myristate acetate/ionomycin and Der p1 allergen. Ionomycin 315-324 tumor necrosis factor Homo sapiens 210-244 18590109-4 2008 Spontaneous production of IL-2, IL-4, IFNgamma (IFNgamma) and TNFalpha (TNFalpha) by CD3(+) and CD3(-) lymphocytes was estimated after 4 hours of incubation in 37 degrees C with phorbol-12-myristate-13-acetate (PMA, 50 ng/ml, Sigma, France), ionomycin (1 mcg/ml, Sigma, France) and brefeldin A (10 mcg/ml, Sigma, France). Ionomycin 242-251 tumor necrosis factor Homo sapiens 72-80 17728323-4 2007 The A323P mutation was found to impair TNF-, IL-1-, LPS- and PMA/ionomycin-induced NF-kappaB activation, as well as to disrupt TRAF6-dependent NEMO polyubiquitination, due to a defective NEMO/TRAF6 interaction. Ionomycin 65-74 tumor necrosis factor Homo sapiens 39-42 17120040-7 2007 In response to PMA/ionomycin, children with AD and asthma symptoms had a significantly lower percentage of CD3(+) T cells producing TNF-alpha. Ionomycin 19-28 tumor necrosis factor Homo sapiens 132-141 17035093-4 2006 This cell subset also expressed TNF-alpha and IFN-gamma, under phorbol-myristate-acetate/ionomycin stimulation. Ionomycin 89-98 tumor necrosis factor Homo sapiens 32-41 17197194-8 2006 In contrast, TNF-alpha gene expression was decreased by approximately 70% in response to ionomycin treatment, but increased in response to addition of CSA. Ionomycin 89-98 tumor necrosis factor Homo sapiens 13-22 12769672-8 2003 This technique was used to monitor the transcription patterns of mRNA encoding TNF-alpha, IL-1beta, and Interferon-gamma in human cell lines or primary PBMC treated with inducers such as PMA, ionomycin, and endotoxin. Ionomycin 192-201 tumor necrosis factor Homo sapiens 79-88 15778356-4 2005 In contrast, IL-4-cultured NK cells produced significant levels of TNF-alpha and GM-CSF only when stimulated with PMA and ionomycin. Ionomycin 122-131 tumor necrosis factor Homo sapiens 67-76 15493878-2 2004 Oxaspirodion inhibited TNF-alpha promoter-driven luciferase reporter gene expression with an IC50 value of 2.5 microg/ml (10 microM) in TPA/ionomycin-stimulated Jurkat T cells. Ionomycin 140-149 tumor necrosis factor Homo sapiens 23-32 15096183-5 2004 The utilization of the PKC inhibitors Go6983, Go6976 and RO-32-0432 demonstrated a role for conventional PKCs (alpha and beta) in the production of TNF-alpha in response to stimulation by lipopolysaccharide and phorbol 12-myristate 13-acetate (PMA)/ionomycin. Ionomycin 249-258 tumor necrosis factor Homo sapiens 148-157 15311213-5 2004 Upon stimulation of normal peripheral blood samples with either phorbol 12-myristate 13 acetate (PMA) plus ionomycin or lipopolysaccharide (LPS), both the number of TNFalpha+ cells and the amount of secreted cytokines progressively increased, the former becoming detectable first. Ionomycin 107-116 tumor necrosis factor Homo sapiens 165-173 15311213-6 2004 After stimulation for 3 h with PMA plus ionomycin, cellular responses were associated with surface TNFalpha expression on the majority of CD3+ T cells and secretion of Th1-associated cytokines: interferon gamma, interleukin (IL)-2, and to a lesser extent IL4. Ionomycin 40-49 tumor necrosis factor Homo sapiens 99-107 11384975-4 2001 Treatment of cells with either ionomycin or endotoxin (lipopolysaccharide) leads to activation of myosin II and increased translocation of TNFR-1 to the plasma membrane independent of TNF signaling. Ionomycin 31-40 tumor necrosis factor Homo sapiens 139-142 12632433-8 2003 RESULTS: Compared with baseline, infliximab treatment induced a significant decrease at 12 weeks in the number of CD4+ and CD8+ T cells that were positive for IFN gamma and TNF alpha upon PMA/ionomycin stimulation (P = 0.005). Ionomycin 192-201 tumor necrosis factor Homo sapiens 173-182 11772508-5 2002 The percentages of CD3+ cells producing TNF-alpha and IFN-gamma were significantly higher in elderly compared to young people (p=0.0049; p=0.0026, respectively) after stimulation with PMA and ionomycin. Ionomycin 192-201 tumor necrosis factor Homo sapiens 40-49 11678905-4 2001 Following re-stimulation in vitro with PMA and ionomycin, CD4+ T cells produced IFNgamma, TNFalpha, TNFbeta, IL-2, IL-4, IL-10 and IL-13. Ionomycin 47-56 tumor necrosis factor Homo sapiens 90-98 14680506-9 2003 Interestingly, macrophages stimulated with phorbol 12-myristate 13-acetate/ionomycin displayed an augmented IL-10 response upon addition of dibutyryl cAMP, with corresponding downregulation in TNF-alpha, suggesting a complex interaction between protein kinase C and protein kinase A in cytokine regulation. Ionomycin 75-84 tumor necrosis factor Homo sapiens 193-202 11113096-7 2000 Upon in vitro stimulation with PDB/ionomycin, they showed predominantly interferon gamma and interleukin (IL)-4 but also tumor necrosis factor alpha and IL-10 production and did not specifically lyse autologous T-cell blasts, B-cell lines, or other autologous or allogeneic target or CD1d-transfected cells. Ionomycin 35-44 tumor necrosis factor Homo sapiens 121-148 11123292-6 2001 Anti-TNF-alpha, anti-IL-2, and anti-IFN-gamma Abs, when added together to PBMC cultures, completely blocked Con A- and partially blocked anti-CD3- and PMA/ionomycin-induced monocyte HA binding. Ionomycin 155-164 tumor necrosis factor Homo sapiens 5-14 11123292-11 2001 Moreover, anti-IFN-gamma and anti-TNF-alpha Abs blocked fixed PMA/ionomycin-activated CD4(+) T cell-induced monocyte HA binding. Ionomycin 66-75 tumor necrosis factor Homo sapiens 34-43 11238213-3 2001 We found that the number of PBMCs stained for tumor necrosis factor alpha and gamma interferon after 6 h of activation was higher when PMA-ionomycin was used for stimulation, while the frequencies of cells positive for interleukin 4 (IL-4) were similar for both stimulators. Ionomycin 139-148 tumor necrosis factor Homo sapiens 46-73 28008484-3 2000 By contrast, they showed the potential to up- or down-regulate the production of TNF evoked by PMA & ionomycin, which was strongly dependent on the time of the stimulation. Ionomycin 105-114 tumor necrosis factor Homo sapiens 81-84 10947068-6 2000 Furthermore, inhibition of TNF-alpha/beta and IL-1alpha/beta, together with CD40 ligand, failed to inhibit EC activation by resting T cells and only inhibited the response to PMA- and ionomycin-activated T cells by 40 +/- 18%. Ionomycin 184-193 tumor necrosis factor Homo sapiens 27-36 10685004-3 2000 Further, in T cells and monocytes that have been stimulated with PMA and ionomycin, the addition of BT results in a dose and time dependent increase in the percentage of high TNFalpha-expressing cells. Ionomycin 73-82 tumor necrosis factor Homo sapiens 175-183 11005615-3 2000 By contrast, they showed the potential to up- or down-regulate the production of TNF evoked by PMA & ionomycin, which was strongly dependent on the time of the stimulation. Ionomycin 105-114 tumor necrosis factor Homo sapiens 81-84 10037239-2 1999 The production of interleukin 2 (IL2), interferon gamma (IFNgamma) and tumor necrosis factor alpha (TNFalpha) was determined in T-helper (Th) and cytotoxic T-cells (CTL) as well as in naive and memory cells after stimulation with phorbol-12-myristate-13-acetate (PMA) and ionomycin under the influence of monensin. Ionomycin 272-281 tumor necrosis factor Homo sapiens 100-108 10403916-2 1999 Apoptosis was also inhibited by ionomycin and zinc, which also increased IL-2 and decreased TNF-alpha production. Ionomycin 32-41 tumor necrosis factor Homo sapiens 92-101 9933633-5 1999 Here we show that extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38-mitogen-activated protein kinase (MAPK) pathways control the transcription and synthesis of TNF-alpha in A3.01 T cells that produce the cytokine upon T cell activation by costimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Ionomycin 339-348 tumor necrosis factor Homo sapiens 194-203 9933633-7 1999 Furthermore, blockage of all three pathways almost abolishes TPA/ionomycin-induced transcriptional activation of the TNF-alpha promoter. Ionomycin 65-74 tumor necrosis factor Homo sapiens 117-126 9933633-8 1999 Selective inhibition of one or more MAPK pathways impairs TNF-alpha induction by TPA/ionomycin, indicating a cooperation between these signal transduction pathways. Ionomycin 85-94 tumor necrosis factor Homo sapiens 58-67 10201926-5 1999 Similar to the effect on TNF-alpha mRNA, stimulation of T cells with PMA + ionomycin greatly increased the stability of CD154 message. Ionomycin 75-84 tumor necrosis factor Homo sapiens 25-34 9973478-6 1999 Furthermore, specific inhibition of CaMKIV by chemical means and by a dominant negative mutant of CaMKIV impairs the ionomycin-induced activity of all three promoters as well as protein expression of CD40L and TNF-alpha. Ionomycin 117-126 tumor necrosis factor Homo sapiens 210-219 10064062-5 1999 Stimulation of transfected Jurkat clones with phorbol-12-myristate-13 alone or in the presence of ionomycin resulted in significant reduction of IL-2R alpha expression, TNF-alpha production, and the induction of transcriptional activity of a pIL-2/Luc construct in both as PKC alpha-reduced clones. Ionomycin 98-107 tumor necrosis factor Homo sapiens 169-178 9469462-3 1998 Protein phosphorylation analysis of cellular MAP kinases indicates that exposure of human neutrophils to chemotactic factor FMLP as well as granulocyte-macrophage CSF, PMA, or ionomycin rapidly induced the activation of p38 and p44/42 MAP kinases, but stimulation with inflammatory cytokine TNF-alpha triggered the activation of p38 MAP kinase only. Ionomycin 176-185 tumor necrosis factor Homo sapiens 291-300 9846011-1 1998 Tumor necrosis factor (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) rapidly primed human neutrophils for enhanced superoxide (O2-) release, and membrane depolarization stimulated by chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine), interleukin 8, concanavalin A (Con A) and ionomycin. Ionomycin 356-365 tumor necrosis factor Homo sapiens 0-21 9518518-11 1998 However, down-regulation of TNF-alpha mRNA levels after ionomycin stimulation provided an indirect proof that IL-10 protein production occurred. Ionomycin 56-65 tumor necrosis factor Homo sapiens 28-37 9469462-7 1998 Moreover, RIA indicates that the activation of cellular p38 MAP kinase was required for the neutrophil IL-8 production stimulated by granulocyte-macrophage CSF or LPS as well as TNF-alpha, but not for that induced by PMA or ionomycin. Ionomycin 224-233 tumor necrosis factor Homo sapiens 178-187 9359732-6 1997 Phorbol ester and ionomycin activation also resulted in a CNI-1493 -induced inhibition of TNF-alpha in monocytes but resistant production of TNF-alpha, IL-2, and IFN-gamma by T cells. Ionomycin 18-27 tumor necrosis factor Homo sapiens 90-99 9359732-6 1997 Phorbol ester and ionomycin activation also resulted in a CNI-1493 -induced inhibition of TNF-alpha in monocytes but resistant production of TNF-alpha, IL-2, and IFN-gamma by T cells. Ionomycin 18-27 tumor necrosis factor Homo sapiens 141-150 7873053-11 1994 However, all clones secreted at least small amounts of IL2, IL4, IFN gamma and TNF alpha, if stimulated by PMA and ionomycin. Ionomycin 115-124 tumor necrosis factor Homo sapiens 79-88 8688912-2 1995 Zymosan, phorbol ester and fluoride induced the formation and accumulation of oxygen radicals intra- and extracellularly, ionomycin and lipopolysaccharide led to an intracellular accumulation of oxygen radicals, while after arachidonic acid and tumor necrosis factor-alpha, no reactive oxygen species were formed. Ionomycin 122-131 tumor necrosis factor Homo sapiens 245-272 7615567-2 1995 Calcium ionophores ionomycin and A23187 reduced the levels of pericellular gelatinolytic activity in both untreated and phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-alpha (TNF alpha)-stimulated cells as determined by degradation of radiolabeled gelatin. Ionomycin 19-28 tumor necrosis factor Homo sapiens 161-188 7615567-2 1995 Calcium ionophores ionomycin and A23187 reduced the levels of pericellular gelatinolytic activity in both untreated and phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-alpha (TNF alpha)-stimulated cells as determined by degradation of radiolabeled gelatin. Ionomycin 19-28 tumor necrosis factor Homo sapiens 190-199 7751024-3 1995 Stimulation of non-IL-4-treated cells with ionomycin (10 microM) for periods of 30 min to 8 hr induced expression of mRNA encoding IL-3, IL-4 and IL-8 but was without effect on levels of mRNA for tumour necrosis factor (TNF)-alpha or beta-actin. Ionomycin 43-52 tumor necrosis factor Homo sapiens 196-230 1721013-9 1991 Io in combination with phorbol-ester induced the secretion of larger amounts of IL-4, GM-CSF, TNF-alpha and low amounts of IFN-gamma. Ionomycin 0-2 tumor necrosis factor Homo sapiens 94-103 1371491-8 1992 When the cells were stimulated by phorbol ester (phorbol 12-myristate 13-acetate, PMA) plus calcium ionophore (ionomycin), FK506 and CsA inhibited, in a dose-dependent manner, the production of IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha. Ionomycin 111-120 tumor necrosis factor Homo sapiens 226-235 7679691-9 1993 LCM-induced proliferation of ionomycin-activated CD56dim NK cells was inhibited 24% by anti-IL-1 heteroantisera and 57% by anti-TNF antisera; a combination of both antisera inhibited proliferation by 73%. Ionomycin 29-38 tumor necrosis factor Homo sapiens 128-131 1786996-9 1991 In contrast, addition of phorbol myristic acid (PMA) led to increased TNF alpha mRNA at 5 h while the combination of PMA and ionomycin increased the level of specific mRNA detected at 1 h, 5 h and 24 h. From genomic analysis ovine TNF alpha appears to exist as a single copy. Ionomycin 125-134 tumor necrosis factor Homo sapiens 231-240 35608955-4 2022 In contrast, following TCR-independent activation using PMA/ionomycin, neonatal cells demonstrated increased expression of CD69, IL-2 and TNF- alpha and equivalent phosphoERK compared to adult cells. Ionomycin 60-69 tumor necrosis factor Homo sapiens 138-148 2307938-4 1990 FACS analysis of highly purified normal human T cells labeled with an anti-TNF mAb revealed that T cells express cell surface TNF when signaled with the synergistic combination of a calcium ionophore, ionomycin, and a protein kinase C activator, 12-o-tetradecanoyl phorbol acetate. Ionomycin 201-210 tumor necrosis factor Homo sapiens 75-78 2307938-4 1990 FACS analysis of highly purified normal human T cells labeled with an anti-TNF mAb revealed that T cells express cell surface TNF when signaled with the synergistic combination of a calcium ionophore, ionomycin, and a protein kinase C activator, 12-o-tetradecanoyl phorbol acetate. Ionomycin 201-210 tumor necrosis factor Homo sapiens 126-129 34867961-9 2021 In stimulating peripheral blood mononuclear cells using PMA plus ionomycin, a high TIM3 level on T cells correlated with low interleukin-2 and tumor necrosis factor-alpha (TNF-alpha) on CD4+ cells and interferon-gamma and TNF-alpha on CD8+ T cells. Ionomycin 65-74 tumor necrosis factor Homo sapiens 143-170 34867961-9 2021 In stimulating peripheral blood mononuclear cells using PMA plus ionomycin, a high TIM3 level on T cells correlated with low interleukin-2 and tumor necrosis factor-alpha (TNF-alpha) on CD4+ cells and interferon-gamma and TNF-alpha on CD8+ T cells. Ionomycin 65-74 tumor necrosis factor Homo sapiens 172-181 34867961-9 2021 In stimulating peripheral blood mononuclear cells using PMA plus ionomycin, a high TIM3 level on T cells correlated with low interleukin-2 and tumor necrosis factor-alpha (TNF-alpha) on CD4+ cells and interferon-gamma and TNF-alpha on CD8+ T cells. Ionomycin 65-74 tumor necrosis factor Homo sapiens 222-231 34804038-7 2021 However, EBV exposure impaired the cytokine (IFN-gamma, IL-2, and TNF-alpha) secretion capability of CD4+ and CD8+ T cells after stimulation with PMA/ionomycin in vitro. Ionomycin 150-159 tumor necrosis factor Homo sapiens 66-75 34135065-6 2021 In humans, CD4+ T cells from rs8005161 heterozygous individuals expressed higher levels of TNF-alpha after PMA/ionomycin stimulation, particularly under pH 6 conditions. Ionomycin 111-120 tumor necrosis factor Homo sapiens 91-100 1907306-4 1991 We found that most clones from both the peripheral blood and CSF express IL-1, IL-2, IL-4, IFN-gamma, or TNF-alpha cytokine mRNA after activation with ionomycin and PMA. Ionomycin 151-160 tumor necrosis factor Homo sapiens 105-114 2522047-2 1989 It is shown here that eight alloreactive CD4+ T cell clones (TCC) secreted significant amounts of TNF-alpha after stimulation with either specific alloantigen or 12-O-tetradecanoylphorbol 13-acetate together with the calcium ionophore ionomycin (up to 50 ng/ml/24 h/10(6) cells) whereas CD8+ TCC failed to do so (max. Ionomycin 235-244 tumor necrosis factor Homo sapiens 98-107 32521784-3 2020 The artificial in vitro activation of CD4+ T lymphocytes by a combination of 12-O-tetradecanoylphorbol-13-acetate and ionomycin, the so-called T/I model, led to an inducible production of cytokines, such as interferon-gamma, tumor necrosis factor-alpha, and interleukin-2. Ionomycin 118-127 tumor necrosis factor Homo sapiens 225-252 31385383-6 2020 Cytokine production of IFN-gamma and TNF-alpha on CD3- CD56+ NK cells in septic patients was also impaired after stimulation by PMA and ionomycin. Ionomycin 136-145 tumor necrosis factor Homo sapiens 37-46 2507324-4 1989 Similarly, using TNF-specific antibody reagents, TNF-secreting cells were detected and quantitated in cell populations obtained from normal lymphoid tissues, bone marrow and peripheral blood, following activation with phorbol myristate acetate and ionomycin. Ionomycin 248-257 tumor necrosis factor Homo sapiens 49-52 2537361-3 1989 TNF-induced O2- release was inhibited by cAMP agonists or ionomycin, and was not accompanied with an increase in cytoplasmic free Ca2+ [( Ca2+]i) and membrane potential changes (depolarization). Ionomycin 58-67 tumor necrosis factor Homo sapiens 0-3 33182021-13 2020 Knocking down NEAT1 expression with an ASO suppressed the expression of CXCL8 and TNF-alpha in PMA/ionomycin-stimulated Jurkat cells. Ionomycin 99-108 tumor necrosis factor Homo sapiens 82-91 33114591-4 2020 We show that venom treatment had a significant immunosuppressive effect, inhibiting the secretion of interleukin (IL)-2 and tumor necrosis factor (TNF) from purified human T cells by 90% or greater following stimulation with mitogen (phorbol 12-myristate 13-acetate and ionomycin) or via cluster of differentiation (CD) receptors, CD3/CD28. Ionomycin 270-279 tumor necrosis factor Homo sapiens 147-150 32912211-6 2020 Further, Tax and M22 protein expression were strongly enhanced by 12-O-Tetradecanoylphorbol-13-Acetate [TPA; Phorbol 12-myristate 13-acetate (PMA)]/ ionomycin, inducers of NF-kappaB and cytokine signaling, but not by tumor necrosis factor alpha (TNF-alpha). Ionomycin 149-158 tumor necrosis factor Homo sapiens 217-244 32912211-6 2020 Further, Tax and M22 protein expression were strongly enhanced by 12-O-Tetradecanoylphorbol-13-Acetate [TPA; Phorbol 12-myristate 13-acetate (PMA)]/ ionomycin, inducers of NF-kappaB and cytokine signaling, but not by tumor necrosis factor alpha (TNF-alpha). Ionomycin 149-158 tumor necrosis factor Homo sapiens 246-255