PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12743567-4 2003 We measured granulocyte-macrophage colony stimulating factor (GM-CSF) production by unstimulated eosinophils and eosinophils stimulated with ionomycin (1 micromol/L), both in the presence and absence of this anti-ICAM-3 antibody. Ionomycin 141-150 colony stimulating factor 2 Homo sapiens 12-60 12743567-8 2003 Antibody binding of ICAM-3, which mimics leukocyte-function antigen-1 binding, had no effect on baseline GM-CSF production but reduced by 80% the production of GM-CSF stimulated by ionomycin (control 1969 pg/mL +/- 1259 SD versus anti-ICAM-3 396 pg/mL +/- 207 SD, n = 8) and reduced GM-CSF mRNA content. Ionomycin 181-190 colony stimulating factor 2 Homo sapiens 160-166 12743567-8 2003 Antibody binding of ICAM-3, which mimics leukocyte-function antigen-1 binding, had no effect on baseline GM-CSF production but reduced by 80% the production of GM-CSF stimulated by ionomycin (control 1969 pg/mL +/- 1259 SD versus anti-ICAM-3 396 pg/mL +/- 207 SD, n = 8) and reduced GM-CSF mRNA content. Ionomycin 181-190 colony stimulating factor 2 Homo sapiens 160-166 18207029-4 2008 Culture in the presence of TNF-alpha induced some differentiation, but only treatment with PMA and ionomycin (with or without prior culture in GM-CSF and IL-4) induced morphological and phenotypic changes consistent with DC-like maturation, and even these maximally differentiated KG-1 cells showed lower levels of surface marker expression, macromolecular endocytosis, and ability to stimulate in allogeneic MLR compared with in vitro monocyte-derived DCs. Ionomycin 99-108 colony stimulating factor 2 Homo sapiens 143-149 15778356-4 2005 In contrast, IL-4-cultured NK cells produced significant levels of TNF-alpha and GM-CSF only when stimulated with PMA and ionomycin. Ionomycin 122-131 colony stimulating factor 2 Homo sapiens 81-87 12743567-4 2003 We measured granulocyte-macrophage colony stimulating factor (GM-CSF) production by unstimulated eosinophils and eosinophils stimulated with ionomycin (1 micromol/L), both in the presence and absence of this anti-ICAM-3 antibody. Ionomycin 141-150 colony stimulating factor 2 Homo sapiens 62-68 7478534-0 1995 ETS1 transactivates the human GM-CSF promoter in Jurkat T cells stimulated with PMA and ionomycin. Ionomycin 88-97 colony stimulating factor 2 Homo sapiens 30-36 11446745-3 2001 Although A23187 and ionomycin stimulated low levels of GM-CSF production, the IL-1beta pathway was cyclosporin A insensitive and did not interact with the calcium pathway. Ionomycin 20-29 colony stimulating factor 2 Homo sapiens 55-61 9824539-3 1998 We show endogenous GM-CSF mRNA is rapidly induced in an eosinophil cell-line (AML14.3D10) after activation with ionomycin. Ionomycin 112-121 colony stimulating factor 2 Homo sapiens 19-25 8625537-9 1996 Similar but transient inhibition of most T-cell products (IL-2, IL-3, IL-4, IL-5, IL-10, TNF-beta and GM-CSF) was noted in the PMA/ionomycin-containing cultures. Ionomycin 131-140 colony stimulating factor 2 Homo sapiens 102-108 9846011-1 1998 Tumor necrosis factor (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) rapidly primed human neutrophils for enhanced superoxide (O2-) release, and membrane depolarization stimulated by chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine), interleukin 8, concanavalin A (Con A) and ionomycin. Ionomycin 356-365 colony stimulating factor 2 Homo sapiens 29-77 9190901-5 1997 We have previously shown that ETS1 can transactivate GM-CSF in Jurkat T cells, but only after the cells have been stimulated by treatment with PMA and ionomycin, agents that mimic T cell activation. Ionomycin 151-160 colony stimulating factor 2 Homo sapiens 53-59 7478534-3 1995 Cis-acting elements with NF kappa B, AP-1 and ETS-like motifs have been identified in the promoter region of the GM-CSF gene, which are important for transcriptional activity following PMA and ionomycin stimulation. Ionomycin 193-202 colony stimulating factor 2 Homo sapiens 113-119 7478534-6 1995 Exogenous ETS1, but not ELF1, can transactivate GM-CSF, through the GM5 site, in a PMA/ionomycin dependent manner. Ionomycin 87-96 colony stimulating factor 2 Homo sapiens 48-54 7478534-9 1995 The GM-CSF promoter, modified in this way to be ETS1 specific, is fully responsive to PMA/ionomycin induction, in addition to ETS1 transactivation in the presence of PMA and ionomycin. Ionomycin 90-99 colony stimulating factor 2 Homo sapiens 4-10 7478534-9 1995 The GM-CSF promoter, modified in this way to be ETS1 specific, is fully responsive to PMA/ionomycin induction, in addition to ETS1 transactivation in the presence of PMA and ionomycin. Ionomycin 174-183 colony stimulating factor 2 Homo sapiens 4-10 7724605-5 1995 In transfection assays with the human GM-CSF promoter, this element has a strong positive effect on the expression of a reporter gene by the human T-cell line Jurkat J6 upon stimulation with phorbol dibutyrate and ionomycin or anti-CD3 antibody. Ionomycin 214-223 colony stimulating factor 2 Homo sapiens 38-44 7631148-4 1995 After treatment with the phorbol ester TPA or the calcium ionophore ionomycin expression of several cytokines, i.e. IL-1 beta, IL-3, IL-6, GM-CSF, TNF-beta and PDGF-A, could be detected. Ionomycin 68-77 colony stimulating factor 2 Homo sapiens 139-145 2553162-1 1989 Recombinant human granulocyte colony-stimulating factor (rhG-CSF) enhanced superoxide release and membrane depolarization in parallel in human granulocytes stimulated by the receptor-mediated agonists, N-formyl-methionyl-leucyl-phenylalanine and wheat germ agglutinin, but not by the Ca2+ ionophore ionomycin and phorbol myristate acetate, which bypass the receptors to stimulate the cells. Ionomycin 299-308 colony stimulating factor 2 Homo sapiens 61-64 1541284-1 1992 Pretreatment of human polymorphonuclear leukocytes with the recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) enhances leukotriene biosynthesis in response to a receptor agonist (e.g. N-formyl-methionyl-leucyl-phenylalanine, fMLP) or a Ca(2+)-ionophore (e.g. ionomycin). Ionomycin 287-296 colony stimulating factor 2 Homo sapiens 78-126 1541284-8 1992 However, staurosporine (200 nM), an inhibitor of protein kinase C totally abolished the priming effect of GM-CSF after stimulation with ionomycin. Ionomycin 136-145 colony stimulating factor 2 Homo sapiens 106-112 1541284-10 1992 These results provide evidence that the enhanced arachidonic acid release in GM-CSF-primed polymorphonuclear leukocytes after stimulation with either fMLP or ionomycin involves activation of protein kinase C which, by a still unknown mechanism, reduces the Ca2+ requirement of phospholipase A2. Ionomycin 158-167 colony stimulating factor 2 Homo sapiens 77-83 1721013-9 1991 Io in combination with phorbol-ester induced the secretion of larger amounts of IL-4, GM-CSF, TNF-alpha and low amounts of IFN-gamma. Ionomycin 0-2 colony stimulating factor 2 Homo sapiens 86-92 1723284-2 1991 Stably transfected lymphoid clones expressing antisense RNA were tested for their ability to synthesize GM-CSF in response to stimulation with phorbol 12-myristate 13-acetate (TPA) and ionomycin. Ionomycin 185-194 colony stimulating factor 2 Homo sapiens 104-110 8673471-3 1995 TPA and ionomycin individually increased GM-CSF mRNA levels in T-lymphocytes and MNCs. Ionomycin 8-17 colony stimulating factor 2 Homo sapiens 41-47 8673471-4 1995 Further, GM-CSF was induced synergistically by TPA plus ionomycin, whereas this combination markedly decreased G-CSF mRNA levels in MNCs. Ionomycin 56-65 colony stimulating factor 2 Homo sapiens 9-15 1875171-4 1991 Moreover, IL-3 and GM-CSF were measurable in ionomycin-stimulated eosinophil supernatants by immunoassay. Ionomycin 45-54 colony stimulating factor 2 Homo sapiens 19-25 2676015-5 1989 On stimulation of BMCs with phorbol myristate acetate (PMA) and phytohemagglutinin or PMA and the calcium ionophore ionomycin, approximately 5% expressed GM-CSF mRNA and approximately 1% IL-3 mRNA. Ionomycin 116-125 colony stimulating factor 2 Homo sapiens 154-160 2676015-11 1989 Treatment of BMCs with high-dose interleukin-2 (IL-2) for 1 week followed by PMA plus ionomycin resulted in a lymphocyte population in which 50% and 3% of cells expressed GM-CSF and IL-3 mRNA, respectively. Ionomycin 86-95 colony stimulating factor 2 Homo sapiens 171-177 25613284-9 2015 The granulocyte-macrophage colony-stimulating factor (CSF2) was by far the most over-expressed gene at both T4 and T24 by comparison to mock-stimulated cells, confirming a major impact of PMA-Ionomycin on cell growth and proliferation. Ionomycin 192-201 colony stimulating factor 2 Homo sapiens 4-52 25613284-9 2015 The granulocyte-macrophage colony-stimulating factor (CSF2) was by far the most over-expressed gene at both T4 and T24 by comparison to mock-stimulated cells, confirming a major impact of PMA-Ionomycin on cell growth and proliferation. Ionomycin 192-201 colony stimulating factor 2 Homo sapiens 54-58