PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1721013-9 1991 Io in combination with phorbol-ester induced the secretion of larger amounts of IL-4, GM-CSF, TNF-alpha and low amounts of IFN-gamma. Ionomycin 0-2 interferon gamma Homo sapiens 123-132 1371491-8 1992 When the cells were stimulated by phorbol ester (phorbol 12-myristate 13-acetate, PMA) plus calcium ionophore (ionomycin), FK506 and CsA inhibited, in a dose-dependent manner, the production of IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha. Ionomycin 111-120 interferon gamma Homo sapiens 212-221 1848581-3 1991 Low levels of IFN-gamma mRNA could be detected transiently after induction with ionomycin alone, whereas simultaneous induction with agents increasing (cAMP)i led to enhanced levels of IFN-gamma mRNA detectable up to 12 h. No IFN-gamma mRNA was detected when the CTL were activated with (cAMP)i-increasing agents alone or with PKC-activating agents such as PMA, suggesting that the transcriptional activation of the IFN-gamma gene was strictly dependent on the Ca2(+)-mediated and cyclosporin A-dependent event. Ionomycin 80-89 interferon gamma Homo sapiens 14-23 1907306-4 1991 We found that most clones from both the peripheral blood and CSF express IL-1, IL-2, IL-4, IFN-gamma, or TNF-alpha cytokine mRNA after activation with ionomycin and PMA. Ionomycin 151-160 interferon gamma Homo sapiens 91-100 33475942-5 2021 IFN-beta-induced STAT1 phosphorylation/dephosphorylation levels and PMA/ionomycin-stimulated intracellular IL-17A/IFN-gamma production in CD4+ T cells were evaluated. Ionomycin 72-81 interferon gamma Homo sapiens 114-123 1971531-5 1990 Cyclosporine (CsA) prevented diC8 and ionomycin-induced expression of IL-2, IFN-gamma, and H-ras genes. Ionomycin 38-47 interferon gamma Homo sapiens 76-85 2107991-5 1990 Reduced IFN-gamma secretion was observed after stimulation with the CD3 monoclonal antibody OKT3, ionomycin + 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or with high levels of IL-2 (200 U/ml). Ionomycin 98-107 interferon gamma Homo sapiens 8-17 7880978-7 1994 IL-2 and IFN-gamma mRNA were detectable in PBMC as early as 3 hours after in vitro stimulation with PMA and ionomycin. Ionomycin 108-117 interferon gamma Homo sapiens 9-18 34867961-9 2021 In stimulating peripheral blood mononuclear cells using PMA plus ionomycin, a high TIM3 level on T cells correlated with low interleukin-2 and tumor necrosis factor-alpha (TNF-alpha) on CD4+ cells and interferon-gamma and TNF-alpha on CD8+ T cells. Ionomycin 65-74 interferon gamma Homo sapiens 201-217 34804038-7 2021 However, EBV exposure impaired the cytokine (IFN-gamma, IL-2, and TNF-alpha) secretion capability of CD4+ and CD8+ T cells after stimulation with PMA/ionomycin in vitro. Ionomycin 150-159 interferon gamma Homo sapiens 45-54 3921610-4 1985 The Ca++ ionophore ionomycin and TPA, used in conjunction, mimicked the effect of specific alloantigen on these T cell clones, i.e., they induced the secretion of IFN-gamma in all clones and the secretion of IL 2 in the T4+ clone. Ionomycin 19-28 interferon gamma Homo sapiens 163-172 31385383-6 2020 Cytokine production of IFN-gamma and TNF-alpha on CD3- CD56+ NK cells in septic patients was also impaired after stimulation by PMA and ionomycin. Ionomycin 136-145 interferon gamma Homo sapiens 23-32 32521784-3 2020 The artificial in vitro activation of CD4+ T lymphocytes by a combination of 12-O-tetradecanoylphorbol-13-acetate and ionomycin, the so-called T/I model, led to an inducible production of cytokines, such as interferon-gamma, tumor necrosis factor-alpha, and interleukin-2. Ionomycin 118-127 interferon gamma Homo sapiens 207-223 31362059-7 2019 Additionally, IL-9Rhigh CD8+ T cells following anti-TCR and PMA + ionomycin stimulation presented higher IL-2 and IL-17 expression, and lower IFN-gamma expression, than IL-9Rlow CD8+ T cells. Ionomycin 66-75 interferon gamma Homo sapiens 142-151 30838000-8 2019 Upon in vitro activation with PMA plus ionomycin or IL12 plus IL18, fewer MAIT cells isolated from the young adult group expressed IFN-gamma, IL17A and Granzyme B then cells from other age groups while the proportion of cells that expressed TNF-alpha was similar. Ionomycin 39-48 interferon gamma Homo sapiens 131-140 30998783-5 2019 As for effector functions, IFNgamma/TNF responses to phosphoantigens or PMA/Ionomycin in Vdelta2+gammadeltaT-cells were weaker in AHB but preserved in CHB, without significant differences for Vdelta1+gammadeltaT-cells. Ionomycin 76-85 interferon gamma Homo sapiens 27-35 30998783-6 2019 Furthermore, early IFNgamma/TNF responses in Vdelta2+ gammadeltaT-cells to brief PMA/Ionomycin stimulation correlated inversely with serum ALT but not HBV DNA. Ionomycin 85-94 interferon gamma Homo sapiens 19-27 30208917-8 2018 However, whereas phorbol 12-myristate 13-acetate/ionomycin stimulation induced the production of both interferon-gamma and IL-17 by breast duct MAIT cells, bacterially exposed breast carcinoma cells elicited a strongly IL-17-biased response. Ionomycin 49-58 interferon gamma Homo sapiens 102-118 30426024-7 2018 Secreted production of IFN-gamma stimulated via CD3 decreased by CH exposure at 30 mug/ml, although the percentage of IFN-gamma + cells induced by PMA/ionomycin did not decrease. Ionomycin 151-160 interferon gamma Homo sapiens 118-127 29292524-8 2018 When stimulated with PMA/ionomycin, CD4+ T cells from women with EP presented significantly higher interferon (IFN)-gamma and interleukin (IL)-17 secretion, and lower transforming growth factor (TGF)-beta secretion. Ionomycin 25-34 interferon gamma Homo sapiens 99-121 29892290-6 2018 However, IL-4 was only weakly expressed, and PMA and ionomycin restimulation favored IFN-gamma over IL-4 expression. Ionomycin 53-62 interferon gamma Homo sapiens 85-94 28837583-6 2017 Analysis of hundreds of individual human peripheral blood NK cells profiled ex vivo revealed that CD56dimCD16+ NK cells are immediate secretors of interferon gamma (IFN-gamma) upon activation by phorbol 12-myristate 13-acetate (PMA) and ionomycin (< 3 h), and that there was no evidence of cooperation between NK cells leading to either synergistic activation or faster IFN-gamma secretion. Ionomycin 237-246 interferon gamma Homo sapiens 147-174 29467761-0 2018 Establishment of the Reference Intervals of Lymphocyte Function in Healthy Adults Based on IFN-gamma Secretion Assay upon Phorbol-12-Myristate-13-Acetate/Ionomycin Stimulation. Ionomycin 154-163 interferon gamma Homo sapiens 91-100 28837583-6 2017 Analysis of hundreds of individual human peripheral blood NK cells profiled ex vivo revealed that CD56dimCD16+ NK cells are immediate secretors of interferon gamma (IFN-gamma) upon activation by phorbol 12-myristate 13-acetate (PMA) and ionomycin (< 3 h), and that there was no evidence of cooperation between NK cells leading to either synergistic activation or faster IFN-gamma secretion. Ionomycin 237-246 interferon gamma Homo sapiens 165-174 28412245-7 2017 However, after PMA + ionomycin stimulation, the degranulation and TNFalpha expression by CXCR5+CD8+ T cells were significantly elevated to a level comparable with CXCR5-CD8+ T cells, whereas the IFNgamma expression by PMA + ionomycin-stimulated CXCR5+CD8+ T cells were significantly higher than that by CXCR5-CD8+ T cells. Ionomycin 21-30 interferon gamma Homo sapiens 195-203 28412245-7 2017 However, after PMA + ionomycin stimulation, the degranulation and TNFalpha expression by CXCR5+CD8+ T cells were significantly elevated to a level comparable with CXCR5-CD8+ T cells, whereas the IFNgamma expression by PMA + ionomycin-stimulated CXCR5+CD8+ T cells were significantly higher than that by CXCR5-CD8+ T cells. Ionomycin 224-233 interferon gamma Homo sapiens 195-203 28157399-5 2017 After PMA+ionomycin stimulation, CXCR5+ CD8+ T cells from CHB patients presented significantly higher transcription level of interferon gamma (IFN-gamma), interleukin 10 (IL-10), and IL-21, as well as higher IL-10 and IL-21 protein secretion, than CXCR5- CD8+ T cells. Ionomycin 10-19 interferon gamma Homo sapiens 125-152 28645309-13 2017 CD4+ and CD8+ T cell populations isolated from the OB during latency were capable of responding to PMA/ionomycin in the production of IFN-gamma similar to T cells from other tissue that possess latent virus including the TG and brain stem. Ionomycin 103-112 interferon gamma Homo sapiens 134-143 28431214-6 2017 The stimulation of tissue with phorbol-12-myristate-13-acetate and ionomycin, recapitulating CAVD microenvironment, resulted in IFN-gamma release. Ionomycin 67-76 interferon gamma Homo sapiens 128-137 26268522-9 2015 CD4+CD25+ enriched PBL stimulated with PMA/Ionomycin in the presence of rIFNgamma were rather resistant to the effect of rIFNgamma, in contrast to CD4+CD25- enriched PBL which showed increasing total Treg with Helios+ Treg switching from IFNgamma- to IFNgamma+ and increasing Helios-IFNgamma+ Treg. Ionomycin 43-52 interferon gamma Homo sapiens 73-81 27082871-9 2016 After stimulation with phorbol-12-myristate 13-acetate and ionomycin, the proportion of T cells producing interferon-gamma or interleukin-2 was higher in the low-IC-TAC group than in the high-IC-TAC group. Ionomycin 59-68 interferon gamma Homo sapiens 106-122 27007115-4 2016 Human NK cells treated with ionomycin lose their ability to degranulate and secrete IFN-gamma in response to a variety of stimuli, but IL-2 stimulation can compensate these defects. Ionomycin 28-37 interferon gamma Homo sapiens 84-93 25948100-5 2015 The levels of interferon-gamma (IFN-gamma) and interleukin-17 (IL-17) secreted by T cells stimulated with PMA and ionomycin were also determined by flow cytometry. Ionomycin 114-123 interferon gamma Homo sapiens 14-30 24706270-8 2014 By stimulation of PBMCs with PMA/ionomycin for 6 h, more than 1-2 % of total CD8 T cells are identified as positive in terms of multifunctionality, thus producing multiple cytokines--IL-2, TNFalpha, and IFNgamma--at single-cell level in case of all healthy donors. Ionomycin 33-42 interferon gamma Homo sapiens 203-211 24554396-7 2014 Re-stimulation with unspecific stimuli (PMA and ionomycin) generated a mixed Th1 (CD4(+)/IFN-gamma(+)) and Th17 (CD4(+)/IL-17(+)) phenotype in comparison with the vehicle-matched group. Ionomycin 48-57 interferon gamma Homo sapiens 89-102 24611083-6 2014 The frequency of IFN-gamma+/tumor necrosis factor-alpha (TNF-alpha)+CD45RO+CD4+ T-cells upon stimulation with phorbol myristate acetate (PMA)/Ionomycin was higher in 2-3 months old infants who received BCG vaccination at birth compared to those who did not. Ionomycin 142-151 interferon gamma Homo sapiens 17-26 23416458-5 2013 Higher levels of IL-17 and IFN-gamma were produced by stimulation with PMA/Ionomycin compared to anti-CD3/anti-CD28. Ionomycin 75-84 interferon gamma Homo sapiens 27-36 23421558-12 2013 In all patients, the interferon-gamma response of T lymphocytes to phorbolmyristate acetate-ionomycin was higher compared with normal donors, but it was further increased after tumor ablation only in prostate cancer patients. Ionomycin 92-101 interferon gamma Homo sapiens 21-37 23129404-12 2013 In response to PMA/ionomycin stimulation, the proportion of IFN-gamma expressing NK-92 cells increased with 100 and 200 ng/ml of leptin. Ionomycin 19-28 interferon gamma Homo sapiens 60-69 23769052-7 2013 Interferon (IFN)-gamma production was induced only by PMA/ionomycin (P < .01) but not by PHA (P = NS). Ionomycin 58-67 interferon gamma Homo sapiens 0-22 23769052-11 2013 Similar to Treg, IFN-gamma-secreting Ts were detected only during PMA/ionomycin stimulation (P < .01), but not during PHA stimulation (P = NS). Ionomycin 70-79 interferon gamma Homo sapiens 17-26 23416458-8 2013 Furthermore the dose response curve for PMA/Ionomycin differed for IL-10 compared to IL-17 and IFN-gamma as it was biphasic with no IL-10 production at higher PMA/Ionomycin concentrations. Ionomycin 44-53 interferon gamma Homo sapiens 95-104 22898392-9 2012 The addition of IGF-I (100ng/ml) to whole blood cultures inhibited the secretion of IFN-gamma from PMA/ionomycin-stimulated cord blood mononuclear cells (CBMC). Ionomycin 103-112 interferon gamma Homo sapiens 84-93 23017670-4 2013 Sixteen hours PMA/Ionomycin stimulation induced iTreg subsets with the phenotype CD4(+)CD25(+)Foxp3(+), CD4(+)IFNgamma(+)Foxp3(+) and CD4(+)CD25(+)IFNgamma(+) co-expressing CD95, CD152, CD178, CD279, Granzyme A, Granzyme B, Perforin, IL-10, and TGFbeta(1). Ionomycin 18-27 interferon gamma Homo sapiens 110-118 23017670-4 2013 Sixteen hours PMA/Ionomycin stimulation induced iTreg subsets with the phenotype CD4(+)CD25(+)Foxp3(+), CD4(+)IFNgamma(+)Foxp3(+) and CD4(+)CD25(+)IFNgamma(+) co-expressing CD95, CD152, CD178, CD279, Granzyme A, Granzyme B, Perforin, IL-10, and TGFbeta(1). Ionomycin 18-27 interferon gamma Homo sapiens 147-155 23555904-7 2013 In comparison, addition of MPA and Cyclosporine A displayed reduced CD107a expression and IFN-gamma production following PMA/Ionomycin stimulation. Ionomycin 125-134 interferon gamma Homo sapiens 90-99 22677201-6 2012 IFN-gamma production was measured upon cell activation using PMA alone or in combination with ionomycin in order to assess functional capacities of the cells. Ionomycin 94-103 interferon gamma Homo sapiens 0-9 22954802-4 2012 CD4(+)CD25(+)IFNgamma(+) PBL separated from PMA/Ionomycin-stimulated PBL of healthy controls inhibited secondary cell cultures of autologous PBL. Ionomycin 48-57 interferon gamma Homo sapiens 13-21 22355730-3 2012 PMA/ionomycin stimulated IFN-gamma+IL-2+TNF-alpha+ CD4 T cell responses were decreased in patients with smear-positive tuberculosis compared to those with smear-negative tuberculosis. Ionomycin 4-13 interferon gamma Homo sapiens 25-34 22268679-4 2012 We examined the effects of risperidone, clozapine, and haloperidol on the production of phorbol myristate acetate and ionomycin-induced interferon-gamma (IFN-gamma)/interleukin (IL)-4 in PBMCs by using intracellular staining. Ionomycin 118-127 interferon gamma Homo sapiens 136-152 22268679-4 2012 We examined the effects of risperidone, clozapine, and haloperidol on the production of phorbol myristate acetate and ionomycin-induced interferon-gamma (IFN-gamma)/interleukin (IL)-4 in PBMCs by using intracellular staining. Ionomycin 118-127 interferon gamma Homo sapiens 154-163 22690041-6 2012 The percentage of CD4(+) cells producing interferon gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha) in response to PMA/ionomycin was significantly reduced in pregnancy. Ionomycin 133-142 interferon gamma Homo sapiens 41-68 22355730-5 2012 Therefore, our findings argue against the notion that Mycobacterium tuberculosis antigen-specific multifunctional Th1 responses in peripheral blood can serve as correlates of protective immunity against tuberculosis; they suggest that the decrease in PMA/ionomycin stimulated IFN-gamma+IL-2+TNF-alpha+ CD4 T cells may be applied for clinical diagnosis of active tuberculosis. Ionomycin 255-264 interferon gamma Homo sapiens 276-285 21795061-7 2011 Ketamine and MK-801 decreased TH1 cells, TH2 cells, IFN-gamma and IL-4 levels but increased the ratio of TH1/TH2 and IFN-gamma/IL-4 in the presence of PMA and ionomycin. Ionomycin 159-168 interferon gamma Homo sapiens 117-126 21994461-8 2011 Following global stimulation with phorbol myristate acetate (PMA)-ionomycin, we noted a significant interleukin 2 (IL-2) deficit in both cervical and blood CD4 T cells from HIV-infected women compared to uninfected women, while gamma interferon (IFN-gamma) production was similar, irrespective of HIV status. Ionomycin 66-75 interferon gamma Homo sapiens 228-255 21795061-6 2011 TH1 cells, TH2 cells, IFN-gamma and IL-4 levels were significantly increased after PMA and ionomycin stimulation. Ionomycin 91-100 interferon gamma Homo sapiens 22-31 21839858-5 2011 Subsequently, over-expression of HMBOX1 significantly inhibited the expression and production of IFN-gamma in NK cells in response to the stimulation of tumor cell K562 or PMA/ionomycin. Ionomycin 176-185 interferon gamma Homo sapiens 97-106 20074601-4 2010 In vitro stimulation of lymph node cells with either TG, staphylococcus enterotoxin B, or phorbol 12-myristate 13-acetate/ionomycin revealed an interferon-gamma expression in T-bet(+) B cells only in the patient and not in controls. Ionomycin 122-131 interferon gamma Homo sapiens 144-160 21664396-2 2011 Herein, the in vitro function of separated CD3(+)CD4(+)CD25(+)Foxp3(+)IFN-gamma(+) PBL that were induced by phorbol 12-myristate 13-acetate (PMA)/ionomycin or alloantigenic stimulation was investigated using cell coculture techniques and flow cytometry. Ionomycin 146-155 interferon gamma Homo sapiens 70-79 21664396-3 2011 CD4(+)CD25(+)Foxp3(+) PBL with intracellular IFN-gamma production increased to 26% in cell cultures stimulated with PMA/ionomycin for 6 hours. Ionomycin 120-129 interferon gamma Homo sapiens 45-54 21664396-6 2011 Addition of enriched CD4(+)CD25(+)IFN-gamma(+) PBL to autologous PMA/ionomycin stimulated PBL decreased blast formation (p < 0.05), indicating suppression of cell proliferation by CD4(+)CD25(+)IFN-gamma(+) PBL. Ionomycin 69-78 interferon gamma Homo sapiens 34-43 20045653-6 2010 Following stimulation with phorbol ester and ionomycin, PBMCs taken from MS patients in relapse developed a more inflammatory profile than those taken from controls or non-relapse patients, with greater expression of CD154, IL-17 and dual expression of IL-17/IFN-gamma. Ionomycin 45-54 interferon gamma Homo sapiens 259-268 18691343-2 2009 Intracellular interleukin-4 (Th2 cytokine) and interferon-gamma (Th1 cytokine) production was assessed in CD4+ T lymphocytes activated by phorbol 12-myristate 13-acetate and ionomycin using flow cytometry. Ionomycin 174-183 interferon gamma Homo sapiens 47-63 20523061-8 2010 In contrast, salbutamol significantly inhibited the spontaneous and ionomycin- plus PMA-stimulated production of IFN-gamma by PBMCs from asthmatics. Ionomycin 68-77 interferon gamma Homo sapiens 113-122 18552380-6 2008 However, IFN-gamma was undetectable unless cells were first stimulated in vitro with PMA and ionomycin, which yielded IFN-gamma in 25% of cells. Ionomycin 93-102 interferon gamma Homo sapiens 9-18 18552380-6 2008 However, IFN-gamma was undetectable unless cells were first stimulated in vitro with PMA and ionomycin, which yielded IFN-gamma in 25% of cells. Ionomycin 93-102 interferon gamma Homo sapiens 118-127 18833003-6 2008 Detection of intracellular cytokine production (interleukin-2, interferon-gamma, and tumor necrosis factor-alpha) upon phorbol 12-myristate 13-acetate-ionomycin stimulation in CD8alphaalpha+ and CD8alphabeta+ T-cells revealed that CD8alphaalpha+ T-cells show a unique cytokine production pattern (tumor necrosis factor-alpha and interferon-gamma production) as compared with CD8alphabeta+ T-cells. Ionomycin 151-160 interferon gamma Homo sapiens 63-112 18833003-6 2008 Detection of intracellular cytokine production (interleukin-2, interferon-gamma, and tumor necrosis factor-alpha) upon phorbol 12-myristate 13-acetate-ionomycin stimulation in CD8alphaalpha+ and CD8alphabeta+ T-cells revealed that CD8alphaalpha+ T-cells show a unique cytokine production pattern (tumor necrosis factor-alpha and interferon-gamma production) as compared with CD8alphabeta+ T-cells. Ionomycin 151-160 interferon gamma Homo sapiens 63-79 18054415-6 2007 Intracellular expression of IFN-gamma and IL-4 by CD3+CD8(-) (Th1 and Th2, respectively) and CD3+CD8+ (Tc1 and Tc2, respectively) was estimated by flow cytometry in peripheral blood cells after stimulation with PMA and ionomycin. Ionomycin 219-228 interferon gamma Homo sapiens 28-37 18637422-6 2008 Blood sample was obtained from patients and examined for the expression of IFN-gamma and TNF-alpha by intracellular staining procedure after stimulation with PMA/ionomycin and allergen. Ionomycin 162-171 interferon gamma Homo sapiens 75-84 18637422-7 2008 RESULTS: Negative correlation was found between expression of IFN-gamma and TNF-alpha after PMA/ionomycin stimulation and allergen stimulation (p > 0,05). Ionomycin 96-105 interferon gamma Homo sapiens 62-71 17546596-7 2007 T-cells were further characterized for IL- 2 and IFN-gamma production induced by PMA/Ionomycin. Ionomycin 85-94 interferon gamma Homo sapiens 49-58 17552910-3 2007 Gliovirin inhibited inducible TNF-alpha promoter activity and synthesis in LPS/IFN-gamma-stimulated macrophages/monocytes and Jurkat T-cells, co-stimulated with 12-O-tetradecanoylphorbol-13-acetate (TPA)/ionomycin, in a dose-dependent manner, with IC(50) values ranging from 0.21 to 2.1 microM (0.1-1 microg/ml). Ionomycin 204-213 interferon gamma Homo sapiens 79-88 16125466-3 2005 The proportion of CD4+ and CD8+ T cells that produced IFN-gamma and IL-4 after stimulation with PMA (Phorbol 12-myristate 13-acetate) and ionomycin was significantly reduced in VL patients compared to sub-clinical and asymptomatic infections or healthy controls. Ionomycin 138-147 interferon gamma Homo sapiens 54-63 17035093-4 2006 This cell subset also expressed TNF-alpha and IFN-gamma, under phorbol-myristate-acetate/ionomycin stimulation. Ionomycin 89-98 interferon gamma Homo sapiens 46-55 17166736-5 2006 In PMA/ionomycin stimulated cells, pre-incubated with increasing concentrations of NIV, transcription was induced in the range 0.06-2 microM; higher concentrations of NIV were found non-stimulating (4 microM) or inhibitory (8 microM) for IFNgamma and IL-2 whereas IL-8 was still induced. Ionomycin 7-16 interferon gamma Homo sapiens 238-246 17152808-6 2006 Blood sample was obtained from patients and examined for the expression of Interleukin 2, 4, and 13 and IFN-gamma by intracellular staining procedure after stimulation with PMA/ionomycin (phorbol 12-myristate 13-acetate) and allergen (D. pteronyssimus, Allergopharma). Ionomycin 177-186 interferon gamma Homo sapiens 104-113 16237110-10 2005 Furthermore, cytolytic functions of PMA and ionomycin-stimulated CD8brightCD56+ T cells against HUVECs were greatly enhanced, by pretreatment of recombinant human IFN-gamma on HUVECs. Ionomycin 44-53 interferon gamma Homo sapiens 163-172 17378751-4 2007 METHODS: Lipopolysaccharide (LPS)and phorbol 12-myristate 13-acetate plus ionomycin (PMA + I)-stimulated PBMC MCP-1 and IFNgamma production were determined in six extremely obese subjects (body mass index [BMI] = 62.4 +/- 8.6 kg/m(2)) before and 1 year after gastric bypass surgery and in six age-matched lean subjects (BMI = 22.7 +/- 1.4 kg/m(2)). Ionomycin 74-83 interferon gamma Homo sapiens 120-128 17309780-3 2007 Upon stimulation with phorbol 12-myristate acetate/ionomycin for 6 h, tuberculin-negative patients had a significantly higher proportion of IFN-gamma-producing CD4(+) T lymphocytes in BALF than in peripheral blood, while both CD4(+) and CD8(+) T-lymphocyte subsets in BALF of tuberculin-positive patients secreted more IFN-gamma than their peripheral blood counterparts. Ionomycin 51-60 interferon gamma Homo sapiens 140-149 17309780-3 2007 Upon stimulation with phorbol 12-myristate acetate/ionomycin for 6 h, tuberculin-negative patients had a significantly higher proportion of IFN-gamma-producing CD4(+) T lymphocytes in BALF than in peripheral blood, while both CD4(+) and CD8(+) T-lymphocyte subsets in BALF of tuberculin-positive patients secreted more IFN-gamma than their peripheral blood counterparts. Ionomycin 51-60 interferon gamma Homo sapiens 319-328 16624619-7 2006 Importantly, expression of IFN-gamma by PMA/Ionomycin-activated CD56(bright) NK cells and CD3+/CD56+ cells was significantly higher in ESN when compared with controls. Ionomycin 44-53 interferon gamma Homo sapiens 27-36 16045733-5 2005 As a result of it the amount of interferon-gamma producing CD7(+) T-cells and the concentration of interferon-gamma in cultural supernatants were maximal in the cell cultures incubated with anti-CD3, interleukin-7 and dexamethasone during the first 68 h and subsequently restimulated with phorbol 12-myristate 13-acetate and ionomycin. Ionomycin 325-334 interferon gamma Homo sapiens 99-115 16045733-5 2005 As a result of it the amount of interferon-gamma producing CD7(+) T-cells and the concentration of interferon-gamma in cultural supernatants were maximal in the cell cultures incubated with anti-CD3, interleukin-7 and dexamethasone during the first 68 h and subsequently restimulated with phorbol 12-myristate 13-acetate and ionomycin. Ionomycin 325-334 interferon gamma Homo sapiens 32-48 15067204-4 2004 Intracellular staining of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) production in CD4+ lymphocytes after phorbol myristate acetate and ionomycin stimulation was performed at the same time points. Ionomycin 147-156 interferon gamma Homo sapiens 51-67 15913800-4 2005 Flow cytometry was used to investigate sequential changes of IFN-gamma producing (Th1) and interleukin-4 producing (Th2) cells from whole blood samples after stimulation with PMA and ionomycin. Ionomycin 183-192 interferon gamma Homo sapiens 61-70 15855253-4 2005 Intracellular expression of IFNgamma and IL-4 was evaluated by multicolor flow-cytometry analysis in peripheral lymphocytes in vitro stimulated by phorbol-12-myristate-13-acetate (PMA) (25 ng/ml) and ionomycin (1 mug/ml) in presence of monensin (5 microm). Ionomycin 200-209 interferon gamma Homo sapiens 28-36 15908253-7 2005 Non-specific stimulation with PMA and ionomycin revealed increased frequencies of CD4+ cells expressing IFN-gamma in controls, while expression of IL-2, IL-4, IL-10, IL-13, and TNF-alpha was not different. Ionomycin 38-47 interferon gamma Homo sapiens 104-113 15849849-4 2005 In vitro IFN-gamma and IL-4 secretion by peripheral blood mononuclear cells after stimulation with ionomycin and phorbol 12 myristate 13 acetate was measured by enzyme-linked immunosorbent assay (ELISA) tests. Ionomycin 99-108 interferon gamma Homo sapiens 9-18 15671558-6 2005 RESULTS: Frequencies of CD3+ T cells producing IFN-gamma (type 1 T cells) in response to phorbol myristate acetate/ionomycin increased (median, 1.8-fold) in patients receiving IL-2 plus HDC but not in those receiving IL-2 alone (P < 0.01 for comparison between arms). Ionomycin 115-124 interferon gamma Homo sapiens 47-56 15627644-11 2004 The production of IL-4 and IFN-gamma was more intense after PMA/ionomycin stimulation than after PMA/ConA stimulation. Ionomycin 64-73 interferon gamma Homo sapiens 27-36 15350507-6 2004 Median frequencies of IFNgamma+ cells obtained after activation by PMA-ionomycin, PHA or SEA-B were 29.3%, 20.0% and 6.8% for CD4+ cells, and 67.8%, 20.6% and 6.8% for CD8+ cells. Ionomycin 71-80 interferon gamma Homo sapiens 22-30 15034076-9 2004 Stimulation of T cells from nonpregnant females with PMA/ionomycin resulted in IkappaBalpha degradation, p65 translocation, and subsequent production of the Th1 cytokines IFN-gamma and IL-2. Ionomycin 57-66 interferon gamma Homo sapiens 171-180 15968830-3 2005 Judging by the cytokine production of interleukin-2 and interferon-gamma in peripheral blood mononuclear cells stimulated by phorbol-myristate-acetate (PMA) and ionomycin, immunosuppressive drugs given for rheumatic disorders during pregnancy do not induce significant immunosuppression in babies. Ionomycin 161-170 interferon gamma Homo sapiens 56-72 14966405-4 2004 METHODS: Expression of NFAT (nuclear factor of activated T cells)-regulated genes (interleukin 2, interferon-gamma, and granulocyte-macrophage colony-stimulating factor) in phorbol myristate acetate/ionomycin-stimulated peripheral blood from healthy volunteers (n=34) and from stable renal (n=25), cardiac (n=26), and liver (n=14) transplant recipients receiving CsA therapy was measured by quantitative real-time reverse transcriptase-polymerase chain reaction before and 2 hr after drug intake. Ionomycin 199-208 interferon gamma Homo sapiens 98-168 14630988-7 2004 In addition, the percentages of T cells secreting IFN-gamma after in vitro stimulation with phorbol myristate acetate and ionomycin was significantly higher in infected fetuses [10% (5-25)] than in healthy fetuses [0.8% (0.6-1.2)] with IFN-gamma being mostly secreted by CD8(+) T cells and to a lesser extend by CD4(+) T cells. Ionomycin 122-131 interferon gamma Homo sapiens 50-59 14630988-10 2004 Indeed, the number of T cells capable of secreting IFN-gamma is strikingly lower after in vitro stimulation with the CMV-specific antigen than after in vitro stimulation with phorbol myristate acetate/ionomycin that bypasses signaling through the T-cell receptor. Ionomycin 201-210 interferon gamma Homo sapiens 51-60 15067204-4 2004 Intracellular staining of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) production in CD4+ lymphocytes after phorbol myristate acetate and ionomycin stimulation was performed at the same time points. Ionomycin 147-156 interferon gamma Homo sapiens 69-78 15792026-5 2004 The intracellular expression of IL-4 and IFN-gamma in CD4+ or CD8+ cells after stimulation with ionomycin/PMA was estimated by flow cytometer (FACSCalibur, Becton Dickinson) and serum levels of both cytokines were assessed with ELISA (R & D Systems) in all subjects. Ionomycin 96-105 interferon gamma Homo sapiens 41-50 12769672-8 2003 This technique was used to monitor the transcription patterns of mRNA encoding TNF-alpha, IL-1beta, and Interferon-gamma in human cell lines or primary PBMC treated with inducers such as PMA, ionomycin, and endotoxin. Ionomycin 192-201 interferon gamma Homo sapiens 104-120 12950238-3 2003 In this study, the intracellular interleukin-4 and interferon-gamma production in CD4+ T-lymphocytes activated by phorbol 12-myristate 13-acetate and ionomycin was assessed via flow cytometry in order to determine the clinical significance of the Th1/Th2 ratio in 42 patients with ITP. Ionomycin 150-159 interferon gamma Homo sapiens 51-67 12534554-6 2003 RESULTS: A significantly lower ratio of IFN-gamma-/IL-4-producing CD4+ T cells after phorbol 12-myristate acetate/ionomycin stimulation was found in patients with atopic cough and atopic asthma compared with normal subjects. Ionomycin 114-123 interferon gamma Homo sapiens 40-49 12714245-4 2003 The production of IL-2 and IFN-gamma by the different subpopulations was studied following stimulation with PMA and Ionomycin. Ionomycin 116-125 interferon gamma Homo sapiens 27-36 12610507-4 2003 Annexin V binding to CD8(+) T cells and PMA/ionomycin-induced IFN-gamma expression were also evaluated in patients and NC. Ionomycin 44-53 interferon gamma Homo sapiens 62-71 12475762-7 2003 The combined application of the Ca(2+) ionophore ionomycin (1 microg/ml) and the phorbol ester phorbol 12-myristate 13-acetate (PMA; 5 microg/ml) stimulated IFN-gamma production, an effect again reversed by hyperosmolarity. Ionomycin 49-58 interferon gamma Homo sapiens 157-166 12508773-3 2002 IL-2, IL-10, and IFN-gamma expression was determined in PMA + ionomycin stimulated T cells on the single cell level. Ionomycin 62-71 interferon gamma Homo sapiens 17-26 12657248-6 2002 Furthermore, IL-2, IL-4, IL-5, and IFN-gamma secretion by Hut 78 cells or CD3(+) T cells stimulated with PMA plus ionomycin or anti-CD3 antibody plus PMA were inhibited in a concentration-dependent manner by BTPs. Ionomycin 114-123 interferon gamma Homo sapiens 35-44 12473063-5 2002 Furthermore, in supernatants from whole blood samples stimulated with phorbol 12-myristate 13-acetate and ionomycin, production of IFN-gamma was significantly decreased, while IL-4 production remained unchanged in AD patients compared with healthy controls. Ionomycin 106-115 interferon gamma Homo sapiens 131-140 12444324-13 2002 After stimulating with PMA-ionomycin we found significant differences in CD4+ lymphocyte production of IL-4 and IFN-gamma with no differences in CD8+ lymphocyte production of either cytokine. Ionomycin 27-36 interferon gamma Homo sapiens 112-121 12167345-3 2002 After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-gamma and IL-4 producing CD8(+) T cells was observed during aging. Ionomycin 59-68 interferon gamma Homo sapiens 103-112 12133875-7 2002 Intracellular expression of IFN-gamma, IL-2, and IL-4 was detected in CD4(+) and CD8(+) T cells after stimulation with phorbol 12-myristate 13-acetate and ionomycin. Ionomycin 155-164 interferon gamma Homo sapiens 28-37 11961080-5 2002 The IC(50) for IL-5 and IL-13 in TPA/ionomycin-stimulated peripheral blood mononuclear cells (PBMC) is 0.7 +/- 0.1 and 0.5 +/- 0.1 microM, respectively, whereas the IC(50) for IFN-gamma is 2.0 +/- 0.4 microM. Ionomycin 37-46 interferon gamma Homo sapiens 176-185 12051768-7 2002 By contrast, PBLs stimulated with PHA or PMA + ionomycin showed a biphasic expression with a sharp increase at 6 h. This induction was closely parallel to IFN-gamma expression and partially to IL-4 and IL-10. Ionomycin 47-56 interferon gamma Homo sapiens 155-164 11174202-5 2001 METHODS: Using triple-color flow cytometry and phorbol 12-myristate acetate/ionomycin stimulation, we measured the production of the intracellular cytokines IFN-gamma and IL-4 in CD4+ and CD8+ T cells separately, which were obtained from peripheral blood and bronchoalveolar lavage fluid (BALF) of 20 patients with sarcoidosis, and compared their cytokine expressions with those of 10 normal subjects. Ionomycin 76-85 interferon gamma Homo sapiens 157-166 11772508-5 2002 The percentages of CD3+ cells producing TNF-alpha and IFN-gamma were significantly higher in elderly compared to young people (p=0.0049; p=0.0026, respectively) after stimulation with PMA and ionomycin. Ionomycin 192-201 interferon gamma Homo sapiens 54-63 11781358-5 2002 Whereas a biased Th1 to Th2 cytokine profile has been suggested to underlie the pathogenesis of IMD in both species, we found defective production of IFN-gamma in our patients after in vitro stimulation of their PBMCs by phorbol-myristate acetate and ionomycin and both IFN-gamma and IL-4 deficiencies in V(alpha)24(+) NK T-enriched cells. Ionomycin 251-260 interferon gamma Homo sapiens 150-159 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Ionomycin 142-151 interferon gamma Homo sapiens 32-48 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Ionomycin 142-151 interferon gamma Homo sapiens 50-59 11678905-4 2001 Following re-stimulation in vitro with PMA and ionomycin, CD4+ T cells produced IFNgamma, TNFalpha, TNFbeta, IL-2, IL-4, IL-10 and IL-13. Ionomycin 47-56 interferon gamma Homo sapiens 80-88 11174202-7 2001 On stimulation with phorbol 12-myristate acetate/ionomycin for 4 hours, in BALF of the patients, but not in peripheral blood, we found a significant increase in the percentage of IFN-gamma-producing CD4+ T cells and a decrease in the percentage of IL-4-producing CD4+ T cells, resulting in a 3.5-fold higher ratio of IFN-gamma/IL-4-producing CD4+ T cells compared with that found in normal subjects. Ionomycin 49-58 interferon gamma Homo sapiens 179-188 11174202-7 2001 On stimulation with phorbol 12-myristate acetate/ionomycin for 4 hours, in BALF of the patients, but not in peripheral blood, we found a significant increase in the percentage of IFN-gamma-producing CD4+ T cells and a decrease in the percentage of IL-4-producing CD4+ T cells, resulting in a 3.5-fold higher ratio of IFN-gamma/IL-4-producing CD4+ T cells compared with that found in normal subjects. Ionomycin 49-58 interferon gamma Homo sapiens 317-326 10644863-9 2000 A significant number of IFN-gamma- and IL-2-expressing T lymphocytes were only detected after 18 hours of PMA/ionomycin stimulation. Ionomycin 110-119 interferon gamma Homo sapiens 24-33 10789681-4 2000 The combination of ionomycin and phorbol ester was able to mimic TCR stimulation to induce IFN-gamma production, although the same treatment triggered granule exocytosis inefficiently. Ionomycin 19-28 interferon gamma Homo sapiens 91-100 10702485-2 2000 We have used a modified version of the ELISPOT assay to monitor changes in the frequency of gamma interferon (IFN-gamma)-producing T cells in a population of lymphocytes responding to a relevant peptide or a nonspecific stimulator, such as phorbol myristate acetate-ionomycin. Ionomycin 266-275 interferon gamma Homo sapiens 92-119 11123292-6 2001 Anti-TNF-alpha, anti-IL-2, and anti-IFN-gamma Abs, when added together to PBMC cultures, completely blocked Con A- and partially blocked anti-CD3- and PMA/ionomycin-induced monocyte HA binding. Ionomycin 155-164 interferon gamma Homo sapiens 36-45 11123292-11 2001 Moreover, anti-IFN-gamma and anti-TNF-alpha Abs blocked fixed PMA/ionomycin-activated CD4(+) T cell-induced monocyte HA binding. Ionomycin 66-75 interferon gamma Homo sapiens 15-24 11007162-4 2000 RESULTS: Activation of lymphocytes with PMA + Ionomycin induced the expression of IL-2 and IFN-gamma in each lymphocyte population. Ionomycin 46-55 interferon gamma Homo sapiens 91-100 10792375-3 2000 Upon cell activation by phorbol myristate acetate and ionomycin, the proportion of CD4+ T cells containing interferon-gamma (IFN-gamma) was found to be greater in the old subjects. Ionomycin 54-63 interferon gamma Homo sapiens 107-123 10792375-3 2000 Upon cell activation by phorbol myristate acetate and ionomycin, the proportion of CD4+ T cells containing interferon-gamma (IFN-gamma) was found to be greater in the old subjects. Ionomycin 54-63 interferon gamma Homo sapiens 125-134 10735602-7 2000 However, by in vitro activation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, peripheral CD4 cells demonstrated a significant decrease of IFN-gamma-producing T helper 1 (Th1) cells and an increase of IL-4-producing T helper 2 (Th2) cells after immunotherapy. Ionomycin 79-88 interferon gamma Homo sapiens 150-159 10685665-10 2000 Gangliosides isolated from tumor supernatants blocked the production of IL-2 and IFN-gamma in response to ionomycin plus phorbol myristate acetate stimulation. Ionomycin 106-115 interferon gamma Homo sapiens 81-90 10191628-4 1999 Flow cytometric detection of intracellular cytokines in tonsillar mononuclear cells stimulated with PMA and ionomycin revealed that CD3 cells produced IL-1 alpha, IL-2, IL-4, IL-8, IFN-gamma and TNF-alpha, and CD19 cells produced IL-1 alpha, IL-6, IL-8 and TFN-alpha. Ionomycin 108-117 interferon gamma Homo sapiens 181-190 10520184-7 1999 The production of IFN-gamma and IL-4 following CD3-CD28 stimulation of RA PB MNC correlated significantly in a ratio 1 : 1 with production following ionomycin-PMA stimulation. Ionomycin 149-158 interferon gamma Homo sapiens 18-27 10193428-2 1999 MS progressive patients showed an increased number of cells producing interferon-gamma (IFN-gamma) after activation with phorbol 12-myristate 13-acetate and ionomycin, compared with patients with clinically inactive forms (P < 0001) and with healthy controls (P = 0001). Ionomycin 157-166 interferon gamma Homo sapiens 70-86 10193428-2 1999 MS progressive patients showed an increased number of cells producing interferon-gamma (IFN-gamma) after activation with phorbol 12-myristate 13-acetate and ionomycin, compared with patients with clinically inactive forms (P < 0001) and with healthy controls (P = 0001). Ionomycin 157-166 interferon gamma Homo sapiens 88-97 10482358-3 1999 Both regulatory regions in these IL-4-primed naive CD4+ T cells, which produce a large amount of IFN-gamma upon stimulation with PMA and ionomycin, were completely methylated in contrast to the same hypomethylated regions in Th1 cells. Ionomycin 137-146 interferon gamma Homo sapiens 97-106 9876227-3 1998 Purified CD45RO CD4 T cells stimulated with PMA and ionomycin secreted higher levels of IL-4 and IFN-gamma, as measured by ELISA, than CD45RA CD4 T cells which secreted little IL-4 or IFN-gamma. Ionomycin 52-61 interferon gamma Homo sapiens 97-106 9876227-3 1998 Purified CD45RO CD4 T cells stimulated with PMA and ionomycin secreted higher levels of IL-4 and IFN-gamma, as measured by ELISA, than CD45RA CD4 T cells which secreted little IL-4 or IFN-gamma. Ionomycin 52-61 interferon gamma Homo sapiens 184-193 9759834-4 1998 However, during the peak of the acute CD4+ T cell response, >20% of the CD4+ T cells secreted IFN-gamma after stimulation with PMA and ionomycin, and >10% of the CD4+ T cells secreted IFN-gamma after stimulation with the LCMV peptides. Ionomycin 138-147 interferon gamma Homo sapiens 97-106 9640627-4 1998 On ionomycin + PMA stimulation, which reveals the intrinsic potential of lymphokine production by T cells, the CD57+ T cell subsets from all individuals produced high amounts of IFN-gamma and TNF-alpha mRNA and protein. Ionomycin 3-12 interferon gamma Homo sapiens 178-187 9814725-8 1998 Interestingly, in PMA + ionomycin stimulated cultures, the atopic cytokine profile was different with more IL-5 (P = 0.0068) and less IFN-gamma production (P = 0.00046) than the control group. Ionomycin 24-33 interferon gamma Homo sapiens 134-143 9682002-6 1998 Lymphocytes which have been exposed to dexamethasone in vitro retained the ability to express CD40L after incubation in medium alone for 48 h. Dexamethasone also inhibited PMA/ionomycin induced IL-2 and IFN-gamma production but not CD25 and CD69 expression. Ionomycin 176-185 interferon gamma Homo sapiens 203-212 9542602-3 1998 In most patients, we found reduced frequencies of T cells recalled to express CD69 and the cytokines interleukin (IL)-4 and interferon-gamma (IFN-gamma) after stimulation of peripheral blood mononuclear cells with phorbol 12-myristate 13-acetate (PMA) and ionomycin, as compared with normal donors. Ionomycin 256-265 interferon gamma Homo sapiens 142-151 7909823-6 1994 Primed cells are enriched in CD45R0hi and CD31- cells, and upon stimulation with PMA+ ionomycin they release significant amounts of IL-2, IFN-gamma, IL-4, IL-5, and IL-10. Ionomycin 86-95 interferon gamma Homo sapiens 138-147 9409644-7 1997 In activated CD8- T cells, IL-2 and interferon-gamma (IFN-gamma) were optimally induced after 10 h stimulation with phorbol 12-myristate acetate (PMA)/ionomycin, and in CD8+ T cells IL-2 was optimally induced after 10 h and IFN-gamma after 6 h. The levels of IL-2 and IFN-gamma in CD8+ and CD8- T cells in four healthy individuals were consistent on four occasions over a 3-month period. Ionomycin 151-160 interferon gamma Homo sapiens 36-52 9409644-7 1997 In activated CD8- T cells, IL-2 and interferon-gamma (IFN-gamma) were optimally induced after 10 h stimulation with phorbol 12-myristate acetate (PMA)/ionomycin, and in CD8+ T cells IL-2 was optimally induced after 10 h and IFN-gamma after 6 h. The levels of IL-2 and IFN-gamma in CD8+ and CD8- T cells in four healthy individuals were consistent on four occasions over a 3-month period. Ionomycin 151-160 interferon gamma Homo sapiens 54-63 7737290-2 1995 IFN-gamma was secreted by the CTL clone in response to the Ca2+ ionophore ionomycin when used in conjunction with either protein kinase C (PKC)-activating phorbol 12-myristate 13-acetate (PMA) or with agents increasing cAMP, including prostaglandin E2. Ionomycin 74-83 interferon gamma Homo sapiens 0-9 7908719-9 1994 RESULTS: In response to phytohemagglutinin, the production of interferon gamma by mononuclear cells from the patients was lower than in normal subjects (P < 0.001), whereas stimulation with ionomycin and phorbol myristate acetate led to normal production of interferon gamma in the patients. Ionomycin 193-202 interferon gamma Homo sapiens 62-78 7908719-9 1994 RESULTS: In response to phytohemagglutinin, the production of interferon gamma by mononuclear cells from the patients was lower than in normal subjects (P < 0.001), whereas stimulation with ionomycin and phorbol myristate acetate led to normal production of interferon gamma in the patients. Ionomycin 193-202 interferon gamma Homo sapiens 261-277 9503684-5 1997 Furthermore, the frequency of T cells which produced IL-4, IL-5 and IFN-gamma stimulated with phorbol myristate acetate and ionomycin increased and reduced in parallel with MFI of EG2-positive cells. Ionomycin 124-133 interferon gamma Homo sapiens 68-77 9359732-6 1997 Phorbol ester and ionomycin activation also resulted in a CNI-1493 -induced inhibition of TNF-alpha in monocytes but resistant production of TNF-alpha, IL-2, and IFN-gamma by T cells. Ionomycin 18-27 interferon gamma Homo sapiens 162-171 8825401-8 1996 In contrast, upon phorbol ester and ionomycin treatment, adult BMC produced more IFN-gamma mRNA than cord BMC. Ionomycin 36-45 interferon gamma Homo sapiens 81-90 7594459-3 1995 These activated T cells produced IL-2, IL-4, IL-5, and IFN-gamma upon stimulation with PMA and ionomycin. Ionomycin 95-104 interferon gamma Homo sapiens 55-64 7554401-5 1995 Importantly, the levels of mRNA encoding c-myc, IL-2R alpha, IL-2 and IFN-gamma were markedly decreased in patient lymphocytes stimulated with PMA+ionomycin as compared to control lymphocytes. Ionomycin 147-156 interferon gamma Homo sapiens 70-79 7622767-2 1995 To examine the pattern of cytokine production associated with elevated IgE levels, phorbol ester plus ionomycin-stimulated production of interleukin (IL)-4, IL-5, and interferon-gamma (IFN-gamma) by blood mononuclear cells from 16 patients with atopic dermatitis was compared with that of 18 healthy subjects. Ionomycin 102-111 interferon gamma Homo sapiens 167-183 7622767-2 1995 To examine the pattern of cytokine production associated with elevated IgE levels, phorbol ester plus ionomycin-stimulated production of interleukin (IL)-4, IL-5, and interferon-gamma (IFN-gamma) by blood mononuclear cells from 16 patients with atopic dermatitis was compared with that of 18 healthy subjects. Ionomycin 102-111 interferon gamma Homo sapiens 185-194 7622767-5 1995 Furthermore, ionomycin plus phorbol ester-stimulated mononuclear cells from patients with atopic dermatitis produced less IL-4 and more IFN-gamma than did cells from healthy subjects. Ionomycin 13-22 interferon gamma Homo sapiens 136-145 7613170-5 1995 IFN-gamma was produced following all three stimuli, but was greatest from cells cultured with PMA and ionomycin. Ionomycin 102-111 interferon gamma Homo sapiens 0-9 7737290-3 1995 We describe that ionomycin induced IFN-gamma gene transcription, which was totally inhibited in the presence of cyclosporin A (CSA), an immunosuppressant forming a calcineurin-inhibiting complex with cyclophilin. Ionomycin 17-26 interferon gamma Homo sapiens 35-44 7737290-6 1995 When transcription of the IFN-gamma gene, initiated in the presence of ionomycin and an agent increasing intracellular cAMP, was inhibited by CSA in the absence of PKC or cAMP-dependent protein kinase activation, the IFN-gamma mRNA was rapidly degraded (half-life = 30 min). Ionomycin 71-80 interferon gamma Homo sapiens 26-35 7737290-6 1995 When transcription of the IFN-gamma gene, initiated in the presence of ionomycin and an agent increasing intracellular cAMP, was inhibited by CSA in the absence of PKC or cAMP-dependent protein kinase activation, the IFN-gamma mRNA was rapidly degraded (half-life = 30 min). Ionomycin 71-80 interferon gamma Homo sapiens 217-226 7875222-3 1995 Initial stimulation of CD4+ cells with anti-CD3 (or the mitogens PHA or PMA+ionomycin) and anti-CD28 monoclonal antibodies induced IL-4, IL-5 and interferon-gamma (IFN-gamma) production and augmented IL-2 production (6- to 11-fold) compared to cells stimulated with anti-CD3 or mitogen alone. Ionomycin 76-85 interferon gamma Homo sapiens 146-162 7875222-3 1995 Initial stimulation of CD4+ cells with anti-CD3 (or the mitogens PHA or PMA+ionomycin) and anti-CD28 monoclonal antibodies induced IL-4, IL-5 and interferon-gamma (IFN-gamma) production and augmented IL-2 production (6- to 11-fold) compared to cells stimulated with anti-CD3 or mitogen alone. Ionomycin 76-85 interferon gamma Homo sapiens 164-173 8228802-5 1993 These IFN-gamma promoter constructs faithfully mirrored expression of the endogenous gene, in that expression required activation both with ionomycin and PMA, was inhibited by cyclosporin A, and was not observed in U937 or THP-1 cells. Ionomycin 140-149 interferon gamma Homo sapiens 6-15 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Ionomycin 48-57 interferon gamma Homo sapiens 151-160 7873053-11 1994 However, all clones secreted at least small amounts of IL2, IL4, IFN gamma and TNF alpha, if stimulated by PMA and ionomycin. Ionomycin 115-124 interferon gamma Homo sapiens 65-74 8233727-11 1993 Considering the low proportion of lymphocytes, stimulation with phorbol myristate acetate in combination with ionomycin resulted in considerable production of the following lymphokines: IL-2, IL-3, IL-4, IL-10, interferon-gamma, tumor necrosis factor-alpha. Ionomycin 110-119 interferon gamma Homo sapiens 211-256 8423347-7 1993 T cell lines generated against Tct proliferated in response to parasite lysate only in the presence of autologous APC and produced IL-2, IL-6, and IFN-gamma but not IL-4 in response to PMA plus ionomycin. Ionomycin 194-203 interferon gamma Homo sapiens 147-156