PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15067204-4 2004 Intracellular staining of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) production in CD4+ lymphocytes after phorbol myristate acetate and ionomycin stimulation was performed at the same time points. Ionomycin 147-156 interleukin 4 Homo sapiens 26-39 15792026-5 2004 The intracellular expression of IL-4 and IFN-gamma in CD4+ or CD8+ cells after stimulation with ionomycin/PMA was estimated by flow cytometer (FACSCalibur, Becton Dickinson) and serum levels of both cytokines were assessed with ELISA (R & D Systems) in all subjects. Ionomycin 96-105 interleukin 4 Homo sapiens 32-36 12534554-6 2003 RESULTS: A significantly lower ratio of IFN-gamma-/IL-4-producing CD4+ T cells after phorbol 12-myristate acetate/ionomycin stimulation was found in patients with atopic cough and atopic asthma compared with normal subjects. Ionomycin 114-123 interleukin 4 Homo sapiens 51-55 12950238-3 2003 In this study, the intracellular interleukin-4 and interferon-gamma production in CD4+ T-lymphocytes activated by phorbol 12-myristate 13-acetate and ionomycin was assessed via flow cytometry in order to determine the clinical significance of the Th1/Th2 ratio in 42 patients with ITP. Ionomycin 150-159 interleukin 4 Homo sapiens 33-46 12444324-13 2002 After stimulating with PMA-ionomycin we found significant differences in CD4+ lymphocyte production of IL-4 and IFN-gamma with no differences in CD8+ lymphocyte production of either cytokine. Ionomycin 27-36 interleukin 4 Homo sapiens 103-107 12473063-5 2002 Furthermore, in supernatants from whole blood samples stimulated with phorbol 12-myristate 13-acetate and ionomycin, production of IFN-gamma was significantly decreased, while IL-4 production remained unchanged in AD patients compared with healthy controls. Ionomycin 106-115 interleukin 4 Homo sapiens 176-180 11174202-7 2001 On stimulation with phorbol 12-myristate acetate/ionomycin for 4 hours, in BALF of the patients, but not in peripheral blood, we found a significant increase in the percentage of IFN-gamma-producing CD4+ T cells and a decrease in the percentage of IL-4-producing CD4+ T cells, resulting in a 3.5-fold higher ratio of IFN-gamma/IL-4-producing CD4+ T cells compared with that found in normal subjects. Ionomycin 49-58 interleukin 4 Homo sapiens 248-252 12133875-7 2002 Intracellular expression of IFN-gamma, IL-2, and IL-4 was detected in CD4(+) and CD8(+) T cells after stimulation with phorbol 12-myristate 13-acetate and ionomycin. Ionomycin 155-164 interleukin 4 Homo sapiens 49-53 12167345-3 2002 After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-gamma and IL-4 producing CD8(+) T cells was observed during aging. Ionomycin 59-68 interleukin 4 Homo sapiens 117-121 12051768-7 2002 By contrast, PBLs stimulated with PHA or PMA + ionomycin showed a biphasic expression with a sharp increase at 6 h. This induction was closely parallel to IFN-gamma expression and partially to IL-4 and IL-10. Ionomycin 47-56 interleukin 4 Homo sapiens 193-197 11678905-4 2001 Following re-stimulation in vitro with PMA and ionomycin, CD4+ T cells produced IFNgamma, TNFalpha, TNFbeta, IL-2, IL-4, IL-10 and IL-13. Ionomycin 47-56 interleukin 4 Homo sapiens 115-119 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Ionomycin 142-151 interleukin 4 Homo sapiens 65-78 11693999-1 2001 In this study, we report on the interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate (PMA) + ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. Ionomycin 142-151 interleukin 4 Homo sapiens 80-84 11174192-10 2001 Ionomycin induced IL-4 secretion by BAL basophils, and this response was reduced with the addition of phorbol myristate acetate. Ionomycin 0-9 interleukin 4 Homo sapiens 18-22 11174202-7 2001 On stimulation with phorbol 12-myristate acetate/ionomycin for 4 hours, in BALF of the patients, but not in peripheral blood, we found a significant increase in the percentage of IFN-gamma-producing CD4+ T cells and a decrease in the percentage of IL-4-producing CD4+ T cells, resulting in a 3.5-fold higher ratio of IFN-gamma/IL-4-producing CD4+ T cells compared with that found in normal subjects. Ionomycin 49-58 interleukin 4 Homo sapiens 327-331 10482358-3 1999 Both regulatory regions in these IL-4-primed naive CD4+ T cells, which produce a large amount of IFN-gamma upon stimulation with PMA and ionomycin, were completely methylated in contrast to the same hypomethylated regions in Th1 cells. Ionomycin 137-146 interleukin 4 Homo sapiens 33-37 11074886-14 2000 Release of IL-2 and IL-4 from peripheral blood mononuclear cell fractions stimulated with phorbolmyristateacetate and ionomycin was significantly increased in patients with trauma but not from those stimulated with toxic shock syndrome toxin-1. Ionomycin 118-127 interleukin 4 Homo sapiens 20-24 10929069-8 2000 In response to ionomycin and dibutyryl cAMP, cord blood cells were more prone than adult lymphocytes to secrete the T helper type 2-derived immunosuppressive cytokines IL-4 and IL-10. Ionomycin 15-24 interleukin 4 Homo sapiens 168-172 10735602-7 2000 However, by in vitro activation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, peripheral CD4 cells demonstrated a significant decrease of IFN-gamma-producing T helper 1 (Th1) cells and an increase of IL-4-producing T helper 2 (Th2) cells after immunotherapy. Ionomycin 79-88 interleukin 4 Homo sapiens 212-216 10518826-6 1999 After ionomycin activation, 60% to 90% of basophils from both control and allergic asthmatic subjects expressed IL-4 and IL-13. Ionomycin 6-15 interleukin 4 Homo sapiens 112-116 10520184-7 1999 The production of IFN-gamma and IL-4 following CD3-CD28 stimulation of RA PB MNC correlated significantly in a ratio 1 : 1 with production following ionomycin-PMA stimulation. Ionomycin 149-158 interleukin 4 Homo sapiens 32-36 8625537-9 1996 Similar but transient inhibition of most T-cell products (IL-2, IL-3, IL-4, IL-5, IL-10, TNF-beta and GM-CSF) was noted in the PMA/ionomycin-containing cultures. Ionomycin 131-140 interleukin 4 Homo sapiens 70-74 10191628-4 1999 Flow cytometric detection of intracellular cytokines in tonsillar mononuclear cells stimulated with PMA and ionomycin revealed that CD3 cells produced IL-1 alpha, IL-2, IL-4, IL-8, IFN-gamma and TNF-alpha, and CD19 cells produced IL-1 alpha, IL-6, IL-8 and TFN-alpha. Ionomycin 108-117 interleukin 4 Homo sapiens 169-173 9620661-3 1998 In fact, the secretion of IL-4 by basophils stimulated with ionomycin alone was down-regulated (30-70%) with the simultaneous addition of PMA. Ionomycin 60-69 interleukin 4 Homo sapiens 26-30 9620661-4 1998 In peripheral blood lymphocytes (PBL), however, the combination of ionomycin and PMA were highly synergistic, resulting in maximum IL-4 release but at a slower rate. Ionomycin 67-76 interleukin 4 Homo sapiens 131-135 9020523-3 1997 Treatment of the cells with the immunosuppressant CsA at 10(-5) M produced a significant inhibition of ionomycin-induced expression of IL-3, IL-4 and IL-8 mRNA, and at 10(-6) M produced a significant inhibition of induced expression of IL-3 and IL-8 but not IL-4. Ionomycin 103-112 interleukin 4 Homo sapiens 141-145 9020523-3 1997 Treatment of the cells with the immunosuppressant CsA at 10(-5) M produced a significant inhibition of ionomycin-induced expression of IL-3, IL-4 and IL-8 mRNA, and at 10(-6) M produced a significant inhibition of induced expression of IL-3 and IL-8 but not IL-4. Ionomycin 103-112 interleukin 4 Homo sapiens 258-262 9020523-5 1997 Treatment of the cells with the corticosteroid DEX at 10(-5) M but not 10(-6) M significantly reduced the ionomycin-induced expression of IL-3 but not IL-4 or IL-8 mRNA. Ionomycin 106-115 interleukin 4 Homo sapiens 151-155 9876227-3 1998 Purified CD45RO CD4 T cells stimulated with PMA and ionomycin secreted higher levels of IL-4 and IFN-gamma, as measured by ELISA, than CD45RA CD4 T cells which secreted little IL-4 or IFN-gamma. Ionomycin 52-61 interleukin 4 Homo sapiens 88-92 9876227-3 1998 Purified CD45RO CD4 T cells stimulated with PMA and ionomycin secreted higher levels of IL-4 and IFN-gamma, as measured by ELISA, than CD45RA CD4 T cells which secreted little IL-4 or IFN-gamma. Ionomycin 52-61 interleukin 4 Homo sapiens 176-180 9856820-5 1998 Increased production of IL-4 and IL-13 in both CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells after 4 h in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin, was more prominent in AD-H patients than in AD-L patients or healthy controls, whereas IFN-gamma-producing CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells were relatively diminished in AD-H patients. Ionomycin 165-174 interleukin 4 Homo sapiens 24-28 9503684-5 1997 Furthermore, the frequency of T cells which produced IL-4, IL-5 and IFN-gamma stimulated with phorbol myristate acetate and ionomycin increased and reduced in parallel with MFI of EG2-positive cells. Ionomycin 124-133 interleukin 4 Homo sapiens 53-57 9158101-4 1997 Upon stimulation of KU812 cells with either phorbol myristate acetate (PMA) or ionomycin (Iono), IL-4, but not IL-13, was produced in response to Iono, while IL-13, but not IL-4, was inducible by PMA. Ionomycin 79-88 interleukin 4 Homo sapiens 97-101 7622767-5 1995 Furthermore, ionomycin plus phorbol ester-stimulated mononuclear cells from patients with atopic dermatitis produced less IL-4 and more IFN-gamma than did cells from healthy subjects. Ionomycin 13-22 interleukin 4 Homo sapiens 122-126 8728355-3 1996 We found that cAMP upregulated IL-4 release from in vivo activated single CD4+ peripheral T cells and CD4+CD8-HSAlowNK1.1- thymocytes stimulated with ionomycin and phorbol ester. Ionomycin 150-159 interleukin 4 Homo sapiens 31-35 7664804-6 1995 The segregation of IL-4 and IL-5 in activated peripheral T cells was found within 72 h of activation upon anti-CD3 or phorbol ester + ionomycin stimulation. Ionomycin 134-143 interleukin 4 Homo sapiens 19-23 8599837-3 1996 Costimulation with ionomycin and PGE2 resulted in the same level of IL-4 promoter activity as the stimulation with ionomycin alone. Ionomycin 19-28 interleukin 4 Homo sapiens 68-72 8599837-3 1996 Costimulation with ionomycin and PGE2 resulted in the same level of IL-4 promoter activity as the stimulation with ionomycin alone. Ionomycin 115-124 interleukin 4 Homo sapiens 68-72 8599837-4 1996 In contrast, costimulation with ionomycin and PMA decreased the activity of the IL-4 promoter by approximately 40% compared to stimulation with ionomycin alone. Ionomycin 32-41 interleukin 4 Homo sapiens 80-84 8599837-4 1996 In contrast, costimulation with ionomycin and PMA decreased the activity of the IL-4 promoter by approximately 40% compared to stimulation with ionomycin alone. Ionomycin 144-153 interleukin 4 Homo sapiens 80-84 8599837-5 1996 Induction of Il-4 promoter by ionomycin was partially inhibited (approximately 50% inhibition) in the presence of as high as 2 microgram/ml cyclosporin A (CsA), an inhibitor of the Ca+/calmodulin-dependent phosphatase calcineurin. Ionomycin 30-39 interleukin 4 Homo sapiens 13-17 8599837-8 1996 Stimulation with ionomycin of cells transfected with low doses of delta CaM-AI, further induced IL-4 promoter activity by approximately 2-fold. Ionomycin 17-26 interleukin 4 Homo sapiens 96-100 7594459-3 1995 These activated T cells produced IL-2, IL-4, IL-5, and IFN-gamma upon stimulation with PMA and ionomycin. Ionomycin 95-104 interleukin 4 Homo sapiens 39-43 7542063-3 1995 IL-4, but not SCF, enhanced ionomycin-induced transcription and secretion of several genes, including the cytokines IL-3, IL-4, granulocyte/macrophage-colony-stimulating factor, IL-8 and the receptor for IL-6 in the human HMC-1 mast cell line. Ionomycin 28-37 interleukin 4 Homo sapiens 0-4 7613170-6 1995 However, IL-4 secretion was only detected in cell cultures stimulated with PMA and ionomycin. Ionomycin 83-92 interleukin 4 Homo sapiens 9-13 7542063-3 1995 IL-4, but not SCF, enhanced ionomycin-induced transcription and secretion of several genes, including the cytokines IL-3, IL-4, granulocyte/macrophage-colony-stimulating factor, IL-8 and the receptor for IL-6 in the human HMC-1 mast cell line. Ionomycin 28-37 interleukin 4 Homo sapiens 122-126 7875222-3 1995 Initial stimulation of CD4+ cells with anti-CD3 (or the mitogens PHA or PMA+ionomycin) and anti-CD28 monoclonal antibodies induced IL-4, IL-5 and interferon-gamma (IFN-gamma) production and augmented IL-2 production (6- to 11-fold) compared to cells stimulated with anti-CD3 or mitogen alone. Ionomycin 76-85 interleukin 4 Homo sapiens 131-135 7751024-3 1995 Stimulation of non-IL-4-treated cells with ionomycin (10 microM) for periods of 30 min to 8 hr induced expression of mRNA encoding IL-3, IL-4 and IL-8 but was without effect on levels of mRNA for tumour necrosis factor (TNF)-alpha or beta-actin. Ionomycin 43-52 interleukin 4 Homo sapiens 19-23 7751024-3 1995 Stimulation of non-IL-4-treated cells with ionomycin (10 microM) for periods of 30 min to 8 hr induced expression of mRNA encoding IL-3, IL-4 and IL-8 but was without effect on levels of mRNA for tumour necrosis factor (TNF)-alpha or beta-actin. Ionomycin 43-52 interleukin 4 Homo sapiens 137-141 7751024-5 1995 More notably, the IL-4 treatment produced a pronounced elevation of mRNA for IL-3 and IL-8 when the cells were subsequently activated with ionomycin. Ionomycin 139-148 interleukin 4 Homo sapiens 18-22 7751024-7 1995 Quantitation of cDNA by competitive polymerase chain reaction (PCR) revealed that the IL-4 treatment produced a sixfold increase in ionomycin-induced levels of cellular IL-3 mRNA, a fourfold increase in induced IL-8 mRNA and less than a twofold increase in induced IL-4 mRNA. Ionomycin 132-141 interleukin 4 Homo sapiens 86-90 7751024-7 1995 Quantitation of cDNA by competitive polymerase chain reaction (PCR) revealed that the IL-4 treatment produced a sixfold increase in ionomycin-induced levels of cellular IL-3 mRNA, a fourfold increase in induced IL-8 mRNA and less than a twofold increase in induced IL-4 mRNA. Ionomycin 132-141 interleukin 4 Homo sapiens 265-269 7751024-8 1995 The IL-4 treatment led to a 15- to 20-fold increase in ionomycin-induced secretion of IL-3 product and a doubling of induced IL-8 product. Ionomycin 55-64 interleukin 4 Homo sapiens 4-8 7748537-4 1995 The release of interleukin-4 (IL-4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol-myristate-acetate and ionomycin. Ionomycin 167-176 interleukin 4 Homo sapiens 15-28 7748537-4 1995 The release of interleukin-4 (IL-4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol-myristate-acetate and ionomycin. Ionomycin 167-176 interleukin 4 Homo sapiens 30-34 8208751-5 1994 IL-4 significantly enhanced the stimulatory actions of ionomycin and EGF on amnion cell PGE2 production. Ionomycin 55-64 interleukin 4 Homo sapiens 0-4 7909823-6 1994 Primed cells are enriched in CD45R0hi and CD31- cells, and upon stimulation with PMA+ ionomycin they release significant amounts of IL-2, IFN-gamma, IL-4, IL-5, and IL-10. Ionomycin 86-95 interleukin 4 Homo sapiens 149-153 8376786-9 1993 More importantly, the alteration in PBMC GR-binding affinity with IL-2 + IL-4 was associated with a functional change in T cell response to methylprednisolone MPN, i.e., a reduced inhibitory effect of MPN on PMA/ionomycin-induced T cell proliferation. Ionomycin 212-221 interleukin 4 Homo sapiens 73-77 7807282-6 1994 Whereas all activators induced significant IFN-gamma secretion, only ionomycin plus PMA stimulation induced large IL-4 secretion. Ionomycin 69-78 interleukin 4 Homo sapiens 114-118 8267044-6 1993 Interleukin-4 significantly enhanced the stimulatory actions of phorbol 12-myristate 13-acetate, ionomycin, and epidermal growth factor but not interleukin-1 beta on prostaglandin E2 production. Ionomycin 97-106 interleukin 4 Homo sapiens 0-13 8294141-4 1993 In addition PBMC incubated with LPGAP released interleukin-4 (IL-4) after pulsing with ionomycin and phorbol myristate acetate. Ionomycin 87-96 interleukin 4 Homo sapiens 47-60 7905497-4 1994 We report that prolonged exposure of immunologically naive and unstimulated human neonatal CD4 T cells to IL-4 or to IL-4 plus either IL-2 or IL-12 markedly affects their cytokine production on primary stimulation with PMA and ionomycin. Ionomycin 227-236 interleukin 4 Homo sapiens 106-110 7905497-4 1994 We report that prolonged exposure of immunologically naive and unstimulated human neonatal CD4 T cells to IL-4 or to IL-4 plus either IL-2 or IL-12 markedly affects their cytokine production on primary stimulation with PMA and ionomycin. Ionomycin 227-236 interleukin 4 Homo sapiens 117-121 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Ionomycin 48-57 interleukin 4 Homo sapiens 77-81 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Ionomycin 48-57 interleukin 4 Homo sapiens 97-101 7905497-7 1994 In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Ionomycin 48-57 interleukin 4 Homo sapiens 97-101 1345920-8 1992 Finally, IL-4 production could only be induced by stimulation with PMA and ionomycin in either resting or activated CD31- CD4 cells. Ionomycin 75-84 interleukin 4 Homo sapiens 9-13 8233727-11 1993 Considering the low proportion of lymphocytes, stimulation with phorbol myristate acetate in combination with ionomycin resulted in considerable production of the following lymphokines: IL-2, IL-3, IL-4, IL-10, interferon-gamma, tumor necrosis factor-alpha. Ionomycin 110-119 interleukin 4 Homo sapiens 198-202 1358967-8 1992 In functional assays, IL-4 production could only be induced in CD45RA-CD27- CD4 clones by stimulation with PMA and ionomycin. Ionomycin 115-124 interleukin 4 Homo sapiens 22-26 1915547-0 1991 Differential inhibition of interleukin 2- and interleukin 4-mediated human B cell proliferation by ionomycin: a possible regulatory role for apoptosis. Ionomycin 99-108 interleukin 4 Homo sapiens 46-59 1371491-8 1992 When the cells were stimulated by phorbol ester (phorbol 12-myristate 13-acetate, PMA) plus calcium ionophore (ionomycin), FK506 and CsA inhibited, in a dose-dependent manner, the production of IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha. Ionomycin 111-120 interleukin 4 Homo sapiens 200-204 1721013-9 1991 Io in combination with phorbol-ester induced the secretion of larger amounts of IL-4, GM-CSF, TNF-alpha and low amounts of IFN-gamma. Ionomycin 0-2 interleukin 4 Homo sapiens 80-84 1915547-3 1991 These studies examined the effect of the calcium ionophore ionomycin, an enhancer of cytoplasmic [Ca2+] levels, on IL2 and IL4-mediated proliferation of anti-mu-stimulated normal human B cells. Ionomycin 59-68 interleukin 4 Homo sapiens 123-126 1915547-4 1991 Ionomycin inhibited the proliferative response of anti-mu-activated B cells to IL4. Ionomycin 0-9 interleukin 4 Homo sapiens 79-82 28053321-3 2017 The functionality of these IL4 haplotypes in the response of immune cells to phorbol 12-myristate 13-acetate (PMA) with Ionomycin and IL-1beta (as inflammatory stimuli) was evaluated. Ionomycin 120-129 interleukin 4 Homo sapiens 27-30 29892290-6 2018 However, IL-4 was only weakly expressed, and PMA and ionomycin restimulation favored IFN-gamma over IL-4 expression. Ionomycin 53-62 interleukin 4 Homo sapiens 100-104 22249152-4 2012 The kinetics of IL-4delta2 and IL-4 production by phorbol myristate acetate (PMA)/ionomycin-activated cells differed, with IL-4delta2 increasing at 48-72h and IL-4 peaking at 24h. Ionomycin 82-91 interleukin 4 Homo sapiens 26-35 22160839-4 2012 Th1 and Th2 cells, levels of INF-gamma, IL-2, IL-4 and the activities of T-bet and GATA3 were significantly increased after incubation with PMA and ionomycin. Ionomycin 148-157 interleukin 4 Homo sapiens 46-50 27119568-3 2016 In this study, we demonstrated that exposure of anti-CD3/CD28 or phorbol 12-myristate 13-acetate (PMA)/ionomycin-activated HuT-78 T lymphocyte cells to 10-20 mM d-lactate significantly increased IL-4 and IL-13 production. Ionomycin 103-112 interleukin 4 Homo sapiens 195-199 22249152-4 2012 The kinetics of IL-4delta2 and IL-4 production by phorbol myristate acetate (PMA)/ionomycin-activated cells differed, with IL-4delta2 increasing at 48-72h and IL-4 peaking at 24h. Ionomycin 82-91 interleukin 4 Homo sapiens 16-20 18207029-4 2008 Culture in the presence of TNF-alpha induced some differentiation, but only treatment with PMA and ionomycin (with or without prior culture in GM-CSF and IL-4) induced morphological and phenotypic changes consistent with DC-like maturation, and even these maximally differentiated KG-1 cells showed lower levels of surface marker expression, macromolecular endocytosis, and ability to stimulate in allogeneic MLR compared with in vitro monocyte-derived DCs. Ionomycin 99-108 interleukin 4 Homo sapiens 154-158 21795061-6 2011 TH1 cells, TH2 cells, IFN-gamma and IL-4 levels were significantly increased after PMA and ionomycin stimulation. Ionomycin 91-100 interleukin 4 Homo sapiens 36-40 21795061-7 2011 Ketamine and MK-801 decreased TH1 cells, TH2 cells, IFN-gamma and IL-4 levels but increased the ratio of TH1/TH2 and IFN-gamma/IL-4 in the presence of PMA and ionomycin. Ionomycin 159-168 interleukin 4 Homo sapiens 127-131 18691343-2 2009 Intracellular interleukin-4 (Th2 cytokine) and interferon-gamma (Th1 cytokine) production was assessed in CD4+ T lymphocytes activated by phorbol 12-myristate 13-acetate and ionomycin using flow cytometry. Ionomycin 174-183 interleukin 4 Homo sapiens 14-27 15849849-4 2005 In vitro IFN-gamma and IL-4 secretion by peripheral blood mononuclear cells after stimulation with ionomycin and phorbol 12 myristate 13 acetate was measured by enzyme-linked immunosorbent assay (ELISA) tests. Ionomycin 99-108 interleukin 4 Homo sapiens 23-27 18054415-6 2007 Intracellular expression of IFN-gamma and IL-4 by CD3+CD8(-) (Th1 and Th2, respectively) and CD3+CD8+ (Tc1 and Tc2, respectively) was estimated by flow cytometry in peripheral blood cells after stimulation with PMA and ionomycin. Ionomycin 219-228 interleukin 4 Homo sapiens 42-46 16143405-7 2006 Upon activation with PMA and ionomycin, the purified CD4+CCR4+ T lymphocytes produced IL-4 and no IFN-gamma. Ionomycin 29-38 interleukin 4 Homo sapiens 86-90 16125466-3 2005 The proportion of CD4+ and CD8+ T cells that produced IFN-gamma and IL-4 after stimulation with PMA (Phorbol 12-myristate 13-acetate) and ionomycin was significantly reduced in VL patients compared to sub-clinical and asymptomatic infections or healthy controls. Ionomycin 138-147 interleukin 4 Homo sapiens 68-72 15913800-4 2005 Flow cytometry was used to investigate sequential changes of IFN-gamma producing (Th1) and interleukin-4 producing (Th2) cells from whole blood samples after stimulation with PMA and ionomycin. Ionomycin 183-192 interleukin 4 Homo sapiens 91-104 16707627-7 2006 In contrast, chelation of intracellular Ca(2+) inhibited both A(2B) receptor- and ionomycin-dependent IL-4 secretion. Ionomycin 82-91 interleukin 4 Homo sapiens 102-106 15778356-4 2005 In contrast, IL-4-cultured NK cells produced significant levels of TNF-alpha and GM-CSF only when stimulated with PMA and ionomycin. Ionomycin 122-131 interleukin 4 Homo sapiens 13-17 15627644-11 2004 The production of IL-4 and IFN-gamma was more intense after PMA/ionomycin stimulation than after PMA/ConA stimulation. Ionomycin 64-73 interleukin 4 Homo sapiens 18-22 15311213-6 2004 After stimulation for 3 h with PMA plus ionomycin, cellular responses were associated with surface TNFalpha expression on the majority of CD3+ T cells and secretion of Th1-associated cytokines: interferon gamma, interleukin (IL)-2, and to a lesser extent IL4. Ionomycin 40-49 interleukin 4 Homo sapiens 255-258 15305939-4 2004 Flow cytometry was used to determine cord blood lymphocyte subtypes and to quantify the intracellular amounts of IL-2, IFN-gamma and IL-4 produced by cord blood CD4(+) helper and CD8(+) cytotoxic T lymphocytes, both spontaneously and after stimulation with phorbol-12-mirystate-13-acetate and ionomycin. Ionomycin 293-302 interleukin 4 Homo sapiens 133-137