PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33162030-7 2021 Interleukin (IL)-2 production in cultured LPL upon stimulation with phorbol 12-myristate 13-acetate and ionomycin for 24 h was significantly lower in SPF mice than in GF mice. Ionomycin 104-113 interleukin 2 Mus musculus 0-18 30815315-4 2019 Compounds 5, 7, and 9 significantly increased IL-2 secretion in phorbol 12-myristate 13-acetate (PMA)/ionomycin (Io)-induced EL-4 T cells. Ionomycin 102-111 interleukin 2 Mus musculus 46-50 30815315-4 2019 Compounds 5, 7, and 9 significantly increased IL-2 secretion in phorbol 12-myristate 13-acetate (PMA)/ionomycin (Io)-induced EL-4 T cells. Ionomycin 113-115 interleukin 2 Mus musculus 46-50 29525600-3 2018 We show that following stimulation of CLL cells with Phorbol myristic acetate, IL-2, the TLR7 agonist imiquimod (P2I) and ionomycin (P2Iio), markedly increased expression of CD54 and CD83 was seen, indicative of B cell activation and a transition to antigen-presenting cells. Ionomycin 122-131 interleukin 2 Mus musculus 79-83 17630204-4 2007 Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. Ionomycin 64-73 interleukin 2 Mus musculus 87-91 18701319-6 2008 Treatment with glutamine increased the production of IL-2 and IFN-gamma from IELs in the presence of PMA plus ionomycin, but had no effect on TNFalpha, IL-4, or IL-5 production. Ionomycin 110-119 interleukin 2 Mus musculus 53-57 29433394-8 2018 The W-EA fraction significantly increased IL-2 secretion in EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io). Ionomycin 130-139 interleukin 2 Mus musculus 42-46 29433394-8 2018 The W-EA fraction significantly increased IL-2 secretion in EL-4 T cells activated with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io). Ionomycin 141-143 interleukin 2 Mus musculus 42-46 17630204-4 2007 Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. Ionomycin 64-73 interleukin 2 Mus musculus 170-174 17630204-4 2007 Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. Ionomycin 75-77 interleukin 2 Mus musculus 87-91 17630204-4 2007 Isoeugenol inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced IL-2 mRNA expression and protein secretion in B6C3F1 mouse splenocytes, and in EL4.IL-2 mouse T-cells, as determined by real-time RT-PCR and ELISA, respectively. Ionomycin 75-77 interleukin 2 Mus musculus 170-174 12827306-6 2003 However, 4T1 or 4T07-IL-2-vaccine-sensitized draining lymph node (DLN) cells, activated ex vivo with bryostatin 1 and ionomycin and expanded in culture, induced complete tumor regressions when adoptively transferred to 4T1 tumor-bearing animals. Ionomycin 118-127 interleukin 2 Mus musculus 21-25 17531200-5 2007 Cabin1 (701-900) blocked both CN-mediated dephosphorylation and nuclear import of NFAT and thus inhibited IL-2 production in response to PMA/ionomycin stimulation. Ionomycin 141-150 interleukin 2 Mus musculus 106-110 16531003-4 2006 Anethole inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced interleukin-2 (IL-2) mRNA expression and protein secretion in EL4 mouse T-cells as determined by quantitative/competitive RT-PCR and ELISA, respectively. Ionomycin 62-71 interleukin 2 Mus musculus 85-98 16531003-4 2006 Anethole inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced interleukin-2 (IL-2) mRNA expression and protein secretion in EL4 mouse T-cells as determined by quantitative/competitive RT-PCR and ELISA, respectively. Ionomycin 62-71 interleukin 2 Mus musculus 100-104 16531003-4 2006 Anethole inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced interleukin-2 (IL-2) mRNA expression and protein secretion in EL4 mouse T-cells as determined by quantitative/competitive RT-PCR and ELISA, respectively. Ionomycin 73-75 interleukin 2 Mus musculus 85-98 16531003-4 2006 Anethole inhibited phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Io)-induced interleukin-2 (IL-2) mRNA expression and protein secretion in EL4 mouse T-cells as determined by quantitative/competitive RT-PCR and ELISA, respectively. Ionomycin 73-75 interleukin 2 Mus musculus 100-104 16472984-4 2006 RESULTS: Levels of IL-2 mRNA in phorbol 12-myristate 13-acetate/ionomycin activated splenocytes and cytokine in T-helper-1 cells were increased by 50 microM of alphaTOC but decreased by 1 mM of alphaTOC. Ionomycin 64-73 interleukin 2 Mus musculus 19-23 16289105-4 2005 Additionally, it inhibited PMA and ionomycin-stimulated IL-2 production in mouse T cells. Ionomycin 35-44 interleukin 2 Mus musculus 56-60 12183516-5 2002 Examination of the ability of splenocytes from uninfected and infected mice to produce IFN-gamma revealed that IL-2(-/-) mice were hyporesponsive to stimulation with anti-CD3 or parasite antigen compared with wild-type mice, and the addition of IL-2 alone or in combination with IL-12 or stimulation with phorbol myristate acetate and ionomycin did not restore the production of IFN-gamma. Ionomycin 335-344 interleukin 2 Mus musculus 111-115 12707339-5 2003 Receptor-independent stimulation of Tgfb1(-/-) T cells by PMA plus ionomycin induces IL-2 production and mitogenic response, and it rescues them from anergy. Ionomycin 67-76 interleukin 2 Mus musculus 85-89 12091710-6 2002 Total peripheral T cell numbers were significantly reduced in CnA beta(-/-) mice and were defective in proliferative capacity and IL-2 production in response to PMA/ionomycin and T cell receptor cross-linking. Ionomycin 165-174 interleukin 2 Mus musculus 130-134 11531960-4 2001 CD4 T cells stimulated with alphaCD3/alphaCD28 or PMA/Ionomycin proliferated and produced principally IL-2 (i.e. a Th1 phenotype), whereas the proliferation of CD4 T cells stimulated with alphaCD3/PMA was apparently driven principally by IL-4 (i.e. a Th2 phenotype). Ionomycin 54-63 interleukin 2 Mus musculus 102-106 10713487-4 2000 Nodularin inhibited PMA plus ionomycin (Io)-induced IL-2 mRNA expression in murine splenocytes and thymocytes as determined by quantitative/competitive RT-PCR. Ionomycin 29-38 interleukin 2 Mus musculus 52-56 11397554-5 2001 Each MC 1 microM suppressed phorbol 12-myristate 13-acetate (PMA) plus ionomycin-induced IL-2 mRNA expression in splenocytes and thymocytes, but not in EL-4 mouse thymoma cells. Ionomycin 71-80 interleukin 2 Mus musculus 89-93 11397554-7 2001 Deprivation of PMA/ionomycin stimuli from activated splenocytes and blockade of new transcription resulted in destabilization of IL-2 mRNA, which was accelerated by MC treatment. Ionomycin 19-28 interleukin 2 Mus musculus 129-133 10713487-4 2000 Nodularin inhibited PMA plus ionomycin (Io)-induced IL-2 mRNA expression in murine splenocytes and thymocytes as determined by quantitative/competitive RT-PCR. Ionomycin 40-42 interleukin 2 Mus musculus 52-56 10640296-1 2000 We previously reported that immunosuppressive cannabinoids inhibited interleukin (IL)-2 steady-state mRNA expression and secretion by phorbol-12-myristate-13-acetate plus ionomycin-activated mouse splenocytes and EL4 murine T-cells. Ionomycin 171-180 interleukin 2 Mus musculus 69-87 9645418-2 1998 When the peripheral mononuclear cells were stimulated with phorbol myristate acetate and ionomycin in the presence of monensin, which blocks the secretion of cytokines, the positive rates for the cytoplasmic IL-2 and IFN-gamma were lower and those for the cytoplasmic IL-4 and IL-10 were higher in SLE than in normal subjects. Ionomycin 89-98 interleukin 2 Mus musculus 208-212 10207608-3 1999 Pretreatment of the splenocytes with both BPB and AACOCF3 suppressed phorbol 12-myristate 13-acetate plus ionomycin-induced IL-2 secretion in a concentration-dependent manner. Ionomycin 106-115 interleukin 2 Mus musculus 124-128 10410976-7 1999 Polyclonal activation of lung T cells from OA/OA mice with 12-myristate 13-acetate (PMA), ionomycin, anti-CD3 mAb, and anti-CD28 mAb resulted in higher percentages of IL-2+ (43%) and IL-5+ (7%) CD4 cells when compared to CD4+ T cells from non-OA sensitized, challenged mice. Ionomycin 90-99 interleukin 2 Mus musculus 167-171 9547358-4 1998 The direct addition of 2-Ara-Gl to mouse splenocyte cultures suppressed phorbol-12-myristate-13-acetate plus ionomycin-induced IL-2 secretion and steady state mRNA expression in a dose-dependent manner. Ionomycin 109-118 interleukin 2 Mus musculus 127-131 9547358-6 1998 2-Ara-Gl at 5, 10, 20, and 50 microM suppressed phorbol-12-myristate-13-acetate plus ionomycin-induced IL-2 promotor activity by 18%, 28%, 39%, and 54%, respectively. Ionomycin 85-94 interleukin 2 Mus musculus 103-107 9551953-3 1998 Preincubating T cells with either EDCI-fixed APC or ionomycin is a proven means of inducing T cell anergy with reduced IL-2 production in response to Ag stimulation. Ionomycin 52-61 interleukin 2 Mus musculus 119-123 9326644-5 1997 Finally, treatment of cells with ionomycin could inhibit interleukin 2-induced STAT protein activation, but this inhibition could be reversed by calpain inhibitors. Ionomycin 33-42 interleukin 2 Mus musculus 57-70 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Ionomycin 65-74 interleukin 2 Mus musculus 83-96 8877730-5 1996 Murine wt p53 derived from pSG5p53cD strongly repressed the IL-2 and IL-4 promoters in both cell lines induced by the phorbol ester TPA and the Ca2+ ionophore ionomycin but not, however, in uninduced cells. Ionomycin 159-168 interleukin 2 Mus musculus 60-64 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Ionomycin 65-74 interleukin 2 Mus musculus 98-102 8662742-6 1996 Likewise, an inhibition of phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced interleukin 2 (IL-2) protein secretion, which correlated to decreased IL-2 gene transcription, was induced by both cannabinol and Delta9-THC. Ionomycin 65-74 interleukin 2 Mus musculus 153-157 8662742-7 1996 Further, cannabinoid treatment also decreased PMA/ionomycin-induced nuclear factor binding to the AP-1 proximal site of the IL-2 promoter. Ionomycin 50-59 interleukin 2 Mus musculus 124-128 8690890-6 1996 However, significant IL-2 production was detected both in anti-CD3 and in PMA + ionomycin-stimulated cultures of 2C+CD4+ T cells. Ionomycin 80-89 interleukin 2 Mus musculus 21-25 8144923-6 1994 dbcAMP inhibited the TPA plus ionomycin-induced transcription of IL-2 and IL-2R genes in EL4 cells, suggesting interference with biochemic events downstream to PI hydrolysis and upstream to transcription of early activation genes. Ionomycin 30-39 interleukin 2 Mus musculus 65-69 8041153-1 1994 Bryostatin 1 (Bryo) is a potent activator of protein kinase C. When T cells are stimulated with Bryo and the calcium ionophore ionomycin (Io), they expand rapidly in low-dose IL-2 (20 U/ml). Ionomycin 127-136 interleukin 2 Mus musculus 175-179 8041153-1 1994 Bryostatin 1 (Bryo) is a potent activator of protein kinase C. When T cells are stimulated with Bryo and the calcium ionophore ionomycin (Io), they expand rapidly in low-dose IL-2 (20 U/ml). Ionomycin 138-140 interleukin 2 Mus musculus 175-179 1602133-9 1992 When unfractionated murine thymocytes were stimulated with phorbol dibutyrate plus ionomycin and cultured with IL-2 + TGF-beta, an increase in CD8 alpha mRNA was seen and greater numbers of CD8+ cells with higher levels of CD8 alpha and CD8 beta surface expression resulted, as compared to controls treated with IL-2 alone. Ionomycin 83-92 interleukin 2 Mus musculus 312-316 1350288-6 1992 In contrast, CD4+ T cells from infected mice could be induced to proliferate by stimulation with PMA and ionomycin resulting in IL-2R up-regulation, IL-2 production, and proliferation. Ionomycin 105-114 interleukin 2 Mus musculus 128-132 7510235-6 1994 These similarities are extended to show that culturing of lpr CD4-CD8- T cells in the presence of IL-2 in combination with phorbol 12-myristate 13-acetate and ionomycin initiates cell cycling and results in the gain of function; re-stimulation now yields IL-2-dependent proliferation in the absence of exogenous IL-2. Ionomycin 159-168 interleukin 2 Mus musculus 255-259 7510235-6 1994 These similarities are extended to show that culturing of lpr CD4-CD8- T cells in the presence of IL-2 in combination with phorbol 12-myristate 13-acetate and ionomycin initiates cell cycling and results in the gain of function; re-stimulation now yields IL-2-dependent proliferation in the absence of exogenous IL-2. Ionomycin 159-168 interleukin 2 Mus musculus 255-259 1521938-6 1992 The results showed that both lectin- and PMA/ionomycin-induced mitogenesis and IL2-dependent proliferation of T-cells from ethanol diet-fed mice were diminished as compared with that of maltose-substitute diet or standard liquid diet. Ionomycin 45-54 interleukin 2 Mus musculus 79-82 1371244-6 1992 Second, PMA and ionomycin induced optimal IL-2 production by both THYB-1 hybrids and BW5147 thymoma cells but only stimulated low or marginal levels of IL-2 production by THYB-2 hybrids. Ionomycin 16-25 interleukin 2 Mus musculus 42-46 1371244-6 1992 Second, PMA and ionomycin induced optimal IL-2 production by both THYB-1 hybrids and BW5147 thymoma cells but only stimulated low or marginal levels of IL-2 production by THYB-2 hybrids. Ionomycin 16-25 interleukin 2 Mus musculus 152-156 1907374-5 1991 However, the cells retained viability and functional potential because stimulation with phorbol 12-myristate 13-acetate and ionomycin bypassed the block in receptor-mediated signaling and induced IL-2 receptor expression and proliferation of the anergic cells. Ionomycin 124-133 interleukin 2 Mus musculus 196-200 1833463-7 1991 Limiting dilution methods show that the ionomycin-sensitive (virgin) subset contains most of the Con A-responsive precursors for cytotoxicity, and most of the cells able to produce IL-2 in responses to Con A or staphylococcal enterotoxin B. Ag-specific helper memory cells are, however, found predominantly in the ionomycin-resistant fraction of the spleen and draining lymph nodes of mice infected with Schistosoma mansoni. Ionomycin 40-49 interleukin 2 Mus musculus 181-185 1833864-10 1991 The production of IL-2 by PMA-ionomycin stimulated T cells was not inhibited by discodermolide; however, the percentage of IL-2 receptor-bearing cells as measured by immunofluorescence with 7D4 antibody, specific for the 55-kDa chain (p55) comprising the murine IL-2 receptor, was reduced. Ionomycin 30-39 interleukin 2 Mus musculus 18-22 1971316-4 1990 However, after stimulation of the T cells with the combination of phorbol-12-myristate-13-acetate (PMA) and ionomycin to bypass CD3, 3 out of 6 old mice still exhibited 2-fold lower proliferative responses than T cells from young mice; IL-2 production by the CD4+ T cells was lower in all old mice tested. Ionomycin 108-117 interleukin 2 Mus musculus 236-240 1972723-6 1990 When Ca2+ influx was induced by calcium ionophore A23187 or ionomycin, the clone produced IL-2 in response to anti-CD3 in the presence of anti-CD4. Ionomycin 60-69 interleukin 2 Mus musculus 90-94 35068054-4 2022 Furthermore, PPP2CB deletion did not affect T cell receptor (TCR)-induced T cell activation or cytokine-induced T cell responses; however, it specifically enhanced phorbol myristate acetate (PMA) plus ionomycin-induced T cell activation with increased cellular proliferation, elevated CD69 and CD25 expression, and enhanced cytokines production (IFN-gamma, IL-2 and TNF). Ionomycin 201-210 interleukin 2 Mus musculus 357-361 2565932-4 1989 Recombinant interleukin 2 (rIL-2), albeit ineffective by itself, leads to vigorous proliferation of DETC when used with either ConA or PMA + ionomycin + IL-1. Ionomycin 141-150 interleukin 2 Mus musculus 12-25 2503037-3 1989 Our results indicate that ionomycin and Con A induce the exposure of both interleukin-2 (IL-2) and insulin receptors on the surface of the lymphocytes within the first 5 min of treatment. Ionomycin 26-35 interleukin 2 Mus musculus 74-87 2503037-3 1989 Our results indicate that ionomycin and Con A induce the exposure of both interleukin-2 (IL-2) and insulin receptors on the surface of the lymphocytes within the first 5 min of treatment. Ionomycin 26-35 interleukin 2 Mus musculus 89-93 2503037-5 1989 c-myc gene expression and DNA synthesis occur in both the ionomycin and Con A-treated lymphocytes when either IL-2 or insulin is present in the culture medium. Ionomycin 58-67 interleukin 2 Mus musculus 110-114 3141500-3 1988 We report here that treatment of cloned murine T lymphocytes with PMA, ionomycin, or the combination led to a dose-dependent inhibition of IL-2-dependent proliferation but did not inhibit lymphokine secretion. Ionomycin 71-80 interleukin 2 Mus musculus 139-143 3135944-0 1988 Antioxidants inhibit proliferation and cell surface expression of receptors for interleukin-2 and transferrin in T lymphocytes stimulated with phorbol myristate acetate and ionomycin. Ionomycin 173-182 interleukin 2 Mus musculus 80-93 3257768-10 1988 In response to PMA and ionomycin (without added IL-2), only RL-73- HSA-cells proliferated and this proliferation was correlated with IL-2 production. Ionomycin 23-32 interleukin 2 Mus musculus 133-137 3131020-3 1988 When splenic lymphocytes derived from aged mice are cultured with Con A plus ionomycin, IL-2 synthesis and IL-2 receptor expression are increased over the values obtained in parallel cultures triggered by Con A alone up to levels equal to that obtained in Con A-activated young cultures. Ionomycin 77-86 interleukin 2 Mus musculus 88-92 3131020-3 1988 When splenic lymphocytes derived from aged mice are cultured with Con A plus ionomycin, IL-2 synthesis and IL-2 receptor expression are increased over the values obtained in parallel cultures triggered by Con A alone up to levels equal to that obtained in Con A-activated young cultures. Ionomycin 77-86 interleukin 2 Mus musculus 107-111 3131020-7 1988 Both IL-2 synthesis and IL-2 receptor expression induced by PMA plus ionomycin reach levels equal or near equal that found in parallel cultures of cells from young animals; however, proliferation is lower than in young adult cultures. Ionomycin 69-78 interleukin 2 Mus musculus 5-9 3131020-7 1988 Both IL-2 synthesis and IL-2 receptor expression induced by PMA plus ionomycin reach levels equal or near equal that found in parallel cultures of cells from young animals; however, proliferation is lower than in young adult cultures. Ionomycin 69-78 interleukin 2 Mus musculus 24-28 3090144-5 1986 In the presence of either suboptimal levels of phorbol ester (PMA) or Ionomycin, the addition of IL 1 resulted in up to an 80-fold enhancement in the amount of IL 2 secreted. Ionomycin 70-79 interleukin 2 Mus musculus 160-164 2945862-4 1986 In a second assay, rIL 1 enhanced proliferation and IL 2 production by 2-4- cells in response to phorbol myristate acetate (PMA) and the calcium ionophore, ionomycin. Ionomycin 156-165 interleukin 2 Mus musculus 52-56 3093570-5 1986 Interestingly, the in vitro growth of PMA + ionomycin-stimulated fetal thymocytes appeared to be IL 2 dependent in that it was inhibited by a monoclonal antibody to the IL 2 receptor. Ionomycin 44-53 interleukin 2 Mus musculus 97-101 3093570-5 1986 Interestingly, the in vitro growth of PMA + ionomycin-stimulated fetal thymocytes appeared to be IL 2 dependent in that it was inhibited by a monoclonal antibody to the IL 2 receptor. Ionomycin 44-53 interleukin 2 Mus musculus 169-173 3100060-5 1986 TPA strongly synergized with ionomycin both for Il-2 secretion and for Il-2 receptor expression whereas Il-1 did not. Ionomycin 29-38 interleukin 2 Mus musculus 48-52 3100060-5 1986 TPA strongly synergized with ionomycin both for Il-2 secretion and for Il-2 receptor expression whereas Il-1 did not. Ionomycin 29-38 interleukin 2 Mus musculus 71-75 3491135-10 1986 It was found that 8B2 T cells cultured with PGM and ionomycin, but not with PGM and PMA, were activated for IL 2 production. Ionomycin 52-61 interleukin 2 Mus musculus 108-112 3081352-10 1986 While we confirmed here that approximately 50-70% freshly isolated immature thymocytes express receptors for IL2, our results indicate that these cells need to be activated (by e.g. PMA + ionomycin) to respond to IL2. Ionomycin 188-197 interleukin 2 Mus musculus 213-216 2999231-4 1985 Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the effect of antigen or interleukin 2 (IL 2) by inducing strong proliferative and alloantigen-specific cytotoxic responses. Ionomycin 36-45 interleukin 2 Mus musculus 144-157 2999231-4 1985 Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the effect of antigen or interleukin 2 (IL 2) by inducing strong proliferative and alloantigen-specific cytotoxic responses. Ionomycin 36-45 interleukin 2 Mus musculus 159-163