PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15115769-3 2004 PGIS was expressed in the microsomes of the infected Sf21 cells after culture in 5 microg/ml hematin-supplemented medium for 72 h. The holoenzyme was isolated from the solubilized microsomal fraction by calcium phosphate gel absorption and purified to homogeneity by DEAE-Sepharose and hydroxyapatite column chromatography. deae-sepharose 267-281 prostaglandin I2 synthase Homo sapiens 0-4