PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 21849978-1 2011 Fumarate hydratase (FH) is an enzyme of the tricarboxylic acid cycle (TCA cycle) that catalyses the hydration of fumarate into malate. malic acid 127-133 fumarate hydratase Homo sapiens 0-18 23886887-5 2013 Our assay uses fumarate hydratase to convert fumarate to malate and uses oxaloacetate decarboxylating malic dehydrogenase to convert malate to pyruvate and to convert NADP(+) to NADPH; the NADPH is detected spectrometrically. malic acid 57-63 fumarate hydratase Homo sapiens 15-33 23886887-5 2013 Our assay uses fumarate hydratase to convert fumarate to malate and uses oxaloacetate decarboxylating malic dehydrogenase to convert malate to pyruvate and to convert NADP(+) to NADPH; the NADPH is detected spectrometrically. malic acid 133-139 fumarate hydratase Homo sapiens 15-33 21849978-1 2011 Fumarate hydratase (FH) is an enzyme of the tricarboxylic acid cycle (TCA cycle) that catalyses the hydration of fumarate into malate. malic acid 127-133 fumarate hydratase Homo sapiens 20-22 17211469-3 2007 Affected individuals harbour a germline mutation of the fumarate hydratase (FH) gene, which encodes an enzyme that catalyses conversion of fumarate to malate in the Kreb"s cycle. malic acid 151-157 fumarate hydratase Homo sapiens 56-74 21498518-1 2011 The enzyme fumarase catalyzes the reversible hydration of fumarate to malate. malic acid 70-76 fumarate hydratase Homo sapiens 11-19 20641983-21 2004 Fumarate, a tricarboxylic acid cycle intermediate, is hydrated to produce malate in a reaction catalyzed by fumarase (or fumarate hydratase). malic acid 74-80 fumarate hydratase Homo sapiens 108-116 20641983-21 2004 Fumarate, a tricarboxylic acid cycle intermediate, is hydrated to produce malate in a reaction catalyzed by fumarase (or fumarate hydratase). malic acid 74-80 fumarate hydratase Homo sapiens 121-139 17211469-3 2007 Affected individuals harbour a germline mutation of the fumarate hydratase (FH) gene, which encodes an enzyme that catalyses conversion of fumarate to malate in the Kreb"s cycle. malic acid 151-157 fumarate hydratase Homo sapiens 76-78 3332992-5 1987 From affinity chromatographic experiments, we have shown that the enzymes of the citric acid cycle and the aspartate-malate shuttle are organized as one huge multi-enzyme complex, and a stoichiometric arrangement of fumarase/malate dehydrogenase/citrate synthase/aspartate aminotransferase has been postulated. malic acid 117-123 fumarate hydratase Homo sapiens 216-224 16154636-1 2005 We demonstrate a facile blue native polyacrylamide gel electrophoresis (BN-PAGE) technique to detect two malate-generating enzymes, namely fumarase (FUM), malate synthase (MS) and four oxaloacetate-forming enzymes, namely pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), citrate lyase (CL) and aspartate aminotransferase (AST). malic acid 105-111 fumarate hydratase Homo sapiens 139-147 16154636-1 2005 We demonstrate a facile blue native polyacrylamide gel electrophoresis (BN-PAGE) technique to detect two malate-generating enzymes, namely fumarase (FUM), malate synthase (MS) and four oxaloacetate-forming enzymes, namely pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), citrate lyase (CL) and aspartate aminotransferase (AST). malic acid 105-111 fumarate hydratase Homo sapiens 149-152 8357797-1 1993 Activation of fumarase by high concentrations of either malate or fumarate, often referred to as negative cooperativity, can be explained without assuming additional sites of substrate action or subunit-subunit interactions. malic acid 56-62 fumarate hydratase Homo sapiens 14-22 15663510-4 2005 Fumarate hydratase is an enzyme that catalyses the conversion of fumarate to malate in the Kreb"s cycle and may also function as a tumour suppressor gene. malic acid 77-83 fumarate hydratase Homo sapiens 0-18 14643060-5 2003 Recently, germline mutations in the FH gene, the product of which (fumarate hydratase) catalyzes the conversion of fumarate to malate in the Krebs cycle, have been detected in a distinct hereditary tumor syndrome, which is characterized by uterine and skin leiomyomatosis and papillary renal cancer. malic acid 127-133 fumarate hydratase Homo sapiens 67-85 11797209-5 2001 Also, L-malic acid produced in free fumarase system satisfied USP criterion, the residual fumaric acid was less than 0.1% and the cost was approximately to that of DL-malic acid produced by chemical synthesis. malic acid 164-177 fumarate hydratase Homo sapiens 36-44 6626216-5 1983 This result confirms the absolute stereochemistry of fumarate-to-malate transformation as catalyzed by the enzyme fumarase. malic acid 65-71 fumarate hydratase Homo sapiens 114-122 24306375-1 1980 Exposure of dark-grown restingEuglena gracilis Klebs var.bacillaris Cori to light, ethanol, or malate produced an increase in the specific activity of fumarase (EC. malic acid 95-101 fumarate hydratase Homo sapiens 151-159 6800407-1 1982 Exposure of dark-grown resting (carbon deficient) Euglena to light, ethanol or malate produced a transient increase in the specific activity of fumarase. malic acid 79-85 fumarate hydratase Homo sapiens 144-152 7333283-8 1981 The steric distribution of the tritium in the samples of malate, as shown by the action of fumarase, revealed that D-(1R)- and D-(1S)-(1-2H1)[1-3H]fructose 6-phosphate was converted into (R)- and (S)-(2H1)[3H]acetyl-phosphate, respectively. malic acid 57-63 fumarate hydratase Homo sapiens 91-99 238846-16 1975 The malate formed from 3S-3-hydroxy[2-3H2]butyryl-CoA on incubation with fumarase lost nearly 50%, that derived from 2R,3S-3-hydroxy[2-2H1,3H1]butyryl-CoA (plus 3R-diastereomer) retained about 26% and that derived from 2S,3S-3-hydroxy[2-1H1,3H1]butyryl-CoA retained about 78% of its total tritium content. malic acid 4-10 fumarate hydratase Homo sapiens 73-81 1156576-12 1975 Reisolation of the malate after fumarase action yielded a 30H/14-C ration of 0.67, indicating 34% retention as expected. malic acid 19-25 fumarate hydratase Homo sapiens 32-40 962872-1 1976 A study of the steady-state kinetics of fumarase over an extended concentration range, using novel methods of analysis, reveals an initial-rate equation of at least fourth degree for malate as substrate at pH 7.0, with no kinetically significant dead-end complex formation even up to concentrations of 100 mM. malic acid 183-189 fumarate hydratase Homo sapiens 40-48 31522598-1 2020 Fumarate hydratase (FH) is a metabolic enzyme that is part of the Krebs cycle and reversibly catalyzes the hydration of fumarate to malate. malic acid 132-138 fumarate hydratase Homo sapiens 0-18 163157-3 1975 In the procedure, fumarate, generated in the reaction, is assayed by conversion to malate with fumarase, determining the malate enzymatically with malate dehydrogenase, and estimating the NADH formed spectrofluorometrically. malic acid 83-89 fumarate hydratase Homo sapiens 95-103 5811774-0 1969 Secondary isotope effects in the dehydration of malic acid by fumarate hydratase. malic acid 48-58 fumarate hydratase Homo sapiens 62-80 33848143-6 2021 The inactivation of FH by mutation causes leiomyomas and renal cell carcinoma by subsequent fumarate buildup and reduction in available malate. malic acid 136-142 fumarate hydratase Homo sapiens 20-22 31522598-1 2020 Fumarate hydratase (FH) is a metabolic enzyme that is part of the Krebs cycle and reversibly catalyzes the hydration of fumarate to malate. malic acid 132-138 fumarate hydratase Homo sapiens 20-22 29226743-2 2017 In the crystal structure of the tetrameric fumarase, it was found that some of the active site residues S145, T147, N188 G364 and H235 had water-mediated hydrogen bonding interactions with pyromellitic acid and citrate which help to the protonation state for the conversion of fumarate to malate. malic acid 289-295 fumarate hydratase Homo sapiens 43-51 31123043-1 2019 Fumarase is a well-characterized TCA cycle enzyme that catalyzes the reversible conversion of fumarate to malate. malic acid 106-112 fumarate hydratase Homo sapiens 0-8 29302811-3 2018 HLRCC is caused by germline mutations in the FH gene, which produces the fumarate hydratase protein that participates in the tricarboxylic acid cycle during the conversion of fumarate to malate. malic acid 187-193 fumarate hydratase Homo sapiens 73-91 29130499-2 2018 Fumarase insufficiencies were associated with increased levels of fumarate, decreased levels of malate and exacerbated salt-induced hypertension. malic acid 96-102 fumarate hydratase Homo sapiens 0-8 28104508-1 2017 In the Krebs cycle, succinate is oxidized to fumarate by succinate dehydrogenase (SDH), followed by the conversion of fumarate to malate by fumarate hydratase (FH). malic acid 130-136 fumarate hydratase Homo sapiens 140-158 31223144-3 2017 (7):833-843) shows that fumarase (FH), a key enzyme responsible for the conversion between fumarate and malate in tricarboxylic acid cycle, is importantly involved in the cellular response to nutrient condition. malic acid 104-110 fumarate hydratase Homo sapiens 24-32 31225435-3 2017 (7):833-843) shows that fumarase (FH), a key enzyme responsible for the conversion between fumarate and malate in tricarboxylic acid cycle, is importantly involved in the cellular response to nutrient condition. malic acid 104-110 fumarate hydratase Homo sapiens 24-32 28538181-5 2017 Knockdown of fumarase in human kidney cells and vascular endothelial cells resulted in decreased levels of malate, aspartate, L-arginine, and NO. malic acid 107-113 fumarate hydratase Homo sapiens 13-21 28104508-1 2017 In the Krebs cycle, succinate is oxidized to fumarate by succinate dehydrogenase (SDH), followed by the conversion of fumarate to malate by fumarate hydratase (FH). malic acid 130-136 fumarate hydratase Homo sapiens 160-162 26380143-3 2015 FH is responsible for catalyzing the conversion of fumarate to malate. malic acid 63-69 fumarate hydratase Homo sapiens 0-2