PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24463527-1 2013 Cystathionine gamma-synthase (CGS) catalyzes the first committed step of methionine (Met) biosynthesis in plants. Methionine 73-83 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-28 25146485-2 2014 The Arabidopsis thaliana cystathionine gamma-synthase (CGS), the first committed enzyme of methionine biosynthesis, is a nuclear-encoded chloroplast protein. Methionine 91-101 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 25-53 30972413-5 2019 In non-allelic mto1-1 (Arabidopsis thaliana cystathionine gamma-synthetase1) and mto3-1 (S-adenosylmethionine synthetase) mutants, both with an increased methionine level, the RAM size was similar to that of the wild type, suggesting that methionine overaccumulation itself did not cause RAM exhaustion in mto2 mutants. Methionine 154-164 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 15-21 27271687-4 2017 To reveal the regulatory role of the Arabidopsis thaliana CYSTATHIONINE gamma-SYNTHASE (AtCGS), methionine main regulatory enzyme, in the synthesis of methionine in seeds, we generated transgenic RNAi seeds with targeted repression of AtCGS during late developmental stages of seeds. Methionine 96-106 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 88-93 27271687-4 2017 To reveal the regulatory role of the Arabidopsis thaliana CYSTATHIONINE gamma-SYNTHASE (AtCGS), methionine main regulatory enzyme, in the synthesis of methionine in seeds, we generated transgenic RNAi seeds with targeted repression of AtCGS during late developmental stages of seeds. Methionine 151-161 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 88-93 27271687-7 2017 The results were compared to transgenic Arabidopsis seeds expressing a feedback-insensitive form of AtCGS (named SSE-AtD-CGS) that were previously showed to accumulate up to sixfold more soluble methionine than wild-type seeds. Methionine 195-205 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 100-105 27271687-7 2017 The results were compared to transgenic Arabidopsis seeds expressing a feedback-insensitive form of AtCGS (named SSE-AtD-CGS) that were previously showed to accumulate up to sixfold more soluble methionine than wild-type seeds. Methionine 195-205 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 102-105 23933995-0 2013 Light and sucrose up-regulate the expression level of Arabidopsis cystathionine gamma-synthase, the key enzyme of methionine biosynthesis pathway. Methionine 114-124 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 66-94 23933995-3 2013 The level of methionine is regulated mainly by cystathionine gamma-synthase (CGS), the first enzyme committed to its biosynthesis. Methionine 13-23 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 47-75 23933995-3 2013 The level of methionine is regulated mainly by cystathionine gamma-synthase (CGS), the first enzyme committed to its biosynthesis. Methionine 13-23 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 77-80 23933995-4 2013 Within our efforts to reveal factors that regulate CGS and methionine content in plants, we have analyzed how light regulates the transcript and protein level of Arabidopsis CGS (AtCGS). Methionine 59-69 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 179-184 24463527-1 2013 Cystathionine gamma-synthase (CGS) catalyzes the first committed step of methionine (Met) biosynthesis in plants. Methionine 85-88 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-28 21335553-1 2011 Expression of the Arabidopsis CGS1 gene, encoding the first committed enzyme of methionine biosynthesis, is feedback-regulated in response to S-adenosyl-L-methionine (AdoMet) at the mRNA level. Methionine 80-90 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 30-34 21677046-8 2011 AtCGS1 codes for cystathionine gamma-synthase (CGS) that catalyzes the first committed step of methionine and S-adenosyl-l-methionine (AdoMet) biosynthesis in plants, and is feedback regulated by mRNA degradation coupled with translation elongation arrest. Methionine 95-105 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-6 21677046-8 2011 AtCGS1 codes for cystathionine gamma-synthase (CGS) that catalyzes the first committed step of methionine and S-adenosyl-l-methionine (AdoMet) biosynthesis in plants, and is feedback regulated by mRNA degradation coupled with translation elongation arrest. Methionine 95-105 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 17-45 18208521-4 2008 Plants co-expressing bAK and the full-length AtCGS (F-AtCGS) have significantly higher methionine and threonine levels compared with the levels found in wild-type plants, but the methionine level does not increase beyond that found in plants expressing F-AtCGS alone. Methionine 87-97 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 45-50 18285355-1 2008 The Arabidopsis thaliana CGS1 gene encodes cystathionine gamma-synthase, the first committed enzyme of methionine biosynthesis in higher plants. Methionine 103-113 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 25-29 18285355-1 2008 The Arabidopsis thaliana CGS1 gene encodes cystathionine gamma-synthase, the first committed enzyme of methionine biosynthesis in higher plants. Methionine 103-113 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 43-71 18208521-5 2008 This finding can be explained through the feedback inhibition regulation mediated by the methionine metabolite on the transcript level of AtCGS. Methionine 89-99 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 138-143 17617175-6 2007 The increased levels of methionine and SMM were correlated with increases in the mRNA and protein levels of AtCGS and a reduced mRNA level for the genes encoding S-adnosylmethionine (SAM) synthase, which converts methionine to SAM. Methionine 24-34 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 108-113 18028551-5 2007 Although the 2 mutants revealed no apparent morphological abnormalities, the overall correlation values in mto1 were much lower than those of the wild-type and tt4 plants, indicating the loss of overall network stability due to the uncontrolled accumulation of methionine. Methionine 261-271 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 107-111 17617175-8 2007 As SAM is a negative regulator of CGS, this reduction leads to higher expression of CGS and consequently to an increased level of methionine. Methionine 130-140 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 34-37 17617175-8 2007 As SAM is a negative regulator of CGS, this reduction leads to higher expression of CGS and consequently to an increased level of methionine. Methionine 130-140 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 84-87 12934018-1 2003 Cystathionine gamma-synthase, the first committed enzyme of methionine biosynthesis in higher plants, is encoded by the CGS1 gene in Arabidopsis thaliana. Methionine 60-70 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 120-124 17059406-3 2006 Here, we report the precise molecular identification of seed proteins by proteomic analysis of wild-type Arabidopsis thaliana and methionine-over-accumulating mutant mto1-1 plants. Methionine 130-140 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 166-170 16507086-1 2006 Cystathionine gamma-synthase (CGS), the first enzyme of methionine biosynthesis in higher plants, plays an important role in the biosynthesis pathway and in regulating methionine metabolism in plant cells. Methionine 56-66 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 30-33 16507086-1 2006 Cystathionine gamma-synthase (CGS), the first enzyme of methionine biosynthesis in higher plants, plays an important role in the biosynthesis pathway and in regulating methionine metabolism in plant cells. Methionine 168-178 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 30-33 16507086-7 2006 We found that the transgenic plants engineered to express only the deleted form of CGS accumulated methionine to a much higher level than those that expressed the full-length CGS. Methionine 99-109 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 83-86 16507086-9 2006 Therefore, although most likely produced from the full-length CGS, the transcript of the deleted form is insensitive to methionine application and its expression may be important for maintaining methionine metabolism even in the presence of a high level of methionine. Methionine 195-205 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 62-65 16507086-9 2006 Therefore, although most likely produced from the full-length CGS, the transcript of the deleted form is insensitive to methionine application and its expression may be important for maintaining methionine metabolism even in the presence of a high level of methionine. Methionine 195-205 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 62-65 16145838-0 2005 The expression level of threonine synthase and cystathionine-gamma-synthase is influenced by the level of both threonine and methionine in Arabidopsis plants. Methionine 125-135 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 47-75 16145838-2 2005 Thus, the enzymes cystathionine-gamma-synthase (CGS) in the methionine pathway and threonine synthase (TS), the last enzyme in the threonine pathway, compete for this common substrate. Methionine 60-70 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 18-46 16145838-2 2005 Thus, the enzymes cystathionine-gamma-synthase (CGS) in the methionine pathway and threonine synthase (TS), the last enzyme in the threonine pathway, compete for this common substrate. Methionine 60-70 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 48-51 16145838-9 2005 While threonine increased the expression level of CGS but reduced that of TS, methionine reduced the expression level of CGS but increased that of TS. Methionine 78-88 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 121-124 15270685-1 2004 Cystathionine gamma-synthase (CGS) catalyses the first committed step of methionine biosynthesis in higher plants. Methionine 73-83 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-28 15270685-1 2004 Cystathionine gamma-synthase (CGS) catalyses the first committed step of methionine biosynthesis in higher plants. Methionine 73-83 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 30-33 15270685-3 2004 Stability of CGS1 mRNA is down-regulated in response to methionine application and the exon 1-coding region of CGS1 itself is necessary and sufficient for this regulation. Methionine 56-66 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 13-17 15270685-4 2004 mto1 (for methionine overaccumulation) mutants of Arabidopsis, which carry single-amino-acid sequence alterations within CGS1 exon 1, are deficient in this regulation and overaccumulate methionine. Methionine 10-20 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-4 15270685-4 2004 mto1 (for methionine overaccumulation) mutants of Arabidopsis, which carry single-amino-acid sequence alterations within CGS1 exon 1, are deficient in this regulation and overaccumulate methionine. Methionine 10-20 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 121-125 15270685-4 2004 mto1 (for methionine overaccumulation) mutants of Arabidopsis, which carry single-amino-acid sequence alterations within CGS1 exon 1, are deficient in this regulation and overaccumulate methionine. Methionine 186-196 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-4 14622248-1 2003 This work proposes a model of the metabolic branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana which involves kinetic competition for phosphohomoserine between the allosteric enzyme threonine synthase and the two-substrate enzyme cystathionine gamma-synthase. Methionine 69-79 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 275-303 17168900-6 2005 As the transgenic alfalfa plants over-expressing AtCGS had significantly higher levels of both soluble and protein-bound methionine and cysteine, they may represent a model and target system for improving the nutritional quality of forage crops. Methionine 121-131 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 49-54 12934018-2 2003 We have shown previously that the stability of the CGS1 mRNA is negatively regulated in response to methionine application [Chiba, Y., Ishikawa, M., Kijima, F., Tyson, R. H., Kim, J., Yamamoto, A., Nambara, E., Leustek, T., Wallsgrove, R. M. & Naito, S. (1999) Science 286, 1371-1374]. Methionine 100-110 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 51-55 12934018-3 2003 To determine whether methionine itself is the effector of the CGS1 exon 1-mediated posttranscriptional regulation, we carried out transfection experiments. Methionine 21-31 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 62-66 11673634-1 2001 Expression of the gene for cystathionine gamma-synthase (CGS), which catalyzes the key step of methionine biosynthesis, is feedback regulated at the level of mRNA stability. Methionine 95-105 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 27-55 14519770-1 2003 Cystathionine gamma-synthase (CGS) catalyses the first committed step in methionine (Met) biosynthesis in higher plants. Methionine 73-83 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-28 14519770-1 2003 Cystathionine gamma-synthase (CGS) catalyses the first committed step in methionine (Met) biosynthesis in higher plants. Methionine 73-83 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 30-33 14519770-1 2003 Cystathionine gamma-synthase (CGS) catalyses the first committed step in methionine (Met) biosynthesis in higher plants. Methionine 85-88 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-28 14519770-1 2003 Cystathionine gamma-synthase (CGS) catalyses the first committed step in methionine (Met) biosynthesis in higher plants. Methionine 85-88 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 30-33 12198195-0 2002 AtMRD1 and AtMRU1, two novel genes with altered mRNA levels in the methionine over-accumulating mto1-1 mutant of Arabidopsis thaliana. Methionine 67-77 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 96-102 12198195-1 2002 The mto1-1 mutant of Arabidopsis thaliana over-accumulates soluble methionine (Met) up to 40-fold higher than that in its Col-0 wild type. Methionine 67-77 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 4-10 12198195-1 2002 The mto1-1 mutant of Arabidopsis thaliana over-accumulates soluble methionine (Met) up to 40-fold higher than that in its Col-0 wild type. Methionine 79-82 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 4-10 11842149-0 2002 The N-terminal region of Arabidopsis cystathionine gamma-synthase plays an important regulatory role in methionine metabolism. Methionine 104-114 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 37-65 11788756-0 2002 Constitutive overexpression of cystathionine gamma-synthase in Arabidopsis leads to accumulation of soluble methionine and S-methylmethionine. Methionine 108-118 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 31-59 11673634-1 2001 Expression of the gene for cystathionine gamma-synthase (CGS), which catalyzes the key step of methionine biosynthesis, is feedback regulated at the level of mRNA stability. Methionine 95-105 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 57-60 11673634-5 2001 Studies with these transgenic lines demonstrated that the coding region of CGS exon 1 is necessary and sufficient for downregulation of its own mRNA accumulation in response to methionine application and that this region acts in cis in this process. Methionine 177-187 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 75-78 11098400-0 2000 Mechanisms to account for maintenance of the soluble methionine pool in transgenic Arabidopsis plants expressing antisense cystathionine gamma-synthase cDNA. Methionine 53-63 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 123-151 11098400-1 2000 To investigate the role of cystathionine gamma-synthase (CGS) in the regulation of methionine synthesis Arabidopsis plants were transformed with a full-length antisense CGS cDNA and transformants analysed. Methionine 83-93 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 27-55 11098400-1 2000 To investigate the role of cystathionine gamma-synthase (CGS) in the regulation of methionine synthesis Arabidopsis plants were transformed with a full-length antisense CGS cDNA and transformants analysed. Methionine 83-93 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 57-60 11098400-8 2000 This indicates that enzymes other than CGS are subjected to a regulatory control by methionine or one of its metabolites. Methionine 84-94 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 39-42 10558994-2 1999 Analysis of Arabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine gamma-synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Methionine 60-70 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 104-132 10558994-2 1999 Analysis of Arabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine gamma-synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Methionine 60-70 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 134-137 9531508-1 1998 Cystathionine gamma-synthase catalyses the first reaction specific for methionine biosynthesis in plants, the gamma-replacement of the phosphoryl substituent of O-phosphohomoserine by cysteine. Methionine 71-81 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 0-28 12232133-0 1994 Isolation of an Arabidopsis thaliana Mutant, mto1, That Overaccumulates Soluble Methionine (Temporal and Spatial Patterns of Soluble Methionine Accumulation). Methionine 80-90 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 45-49 12232133-0 1994 Isolation of an Arabidopsis thaliana Mutant, mto1, That Overaccumulates Soluble Methionine (Temporal and Spatial Patterns of Soluble Methionine Accumulation). Methionine 133-143 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein Arabidopsis thaliana 45-49