PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24447464-1 2014 11 beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) converts cortisone to cortisol in a NADPH dependent manner. NADP 94-99 RNA, U1 small nuclear 1 Homo sapiens 0-56 29073307-1 2017 INTRODUCTION: Inactivating mutations in the enzyme hexose-6-phosphate dehydrogenase (H6PDH), the enzyme responsible for NADPH generation playing critical role in 11-hydroxysteroid dehydrogenase type 1 (11b-HSD1) activity, cause apparent cortisone reductase deficiency (ACRD). NADP 120-125 RNA, U1 small nuclear 1 Homo sapiens 206-210 21163329-2 2011 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) regulates the key hepatic gluconeogenic enzymes including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) through the amplification of glucocorticoid receptor (GR) - mediated tissue glucocorticoid action, and is crucially dependent on hexose-6-phosphate dehydrogenase (H6PDH) - generating NADPH system. NADP 375-380 RNA, U1 small nuclear 1 Homo sapiens 0-55 21138273-1 2011 17beta-Hydroxysteroid dehydrogenase type 1 (17beta-HSD1) is an enzyme that catalyzes NADPH-dependent reduction of the weak estrogen, estrone, into the most potent estrogen, estradiol, which exerts proliferative effects via the estrogen receptors. NADP 85-90 RNA, U1 small nuclear 1 Homo sapiens 0-55 19824648-2 2009 17beta-Hydroxysteroid dehydrogenase type 1 (17beta-HSD1) is a key enzyme in the biosynthesis of female sex steroids, catalyzing the NADPH-dependent reduction of estrone into biologically active estradiol. NADP 132-137 RNA, U1 small nuclear 1 Homo sapiens 0-55 20826560-2 2010 Glucocorticoid activation by 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) depends on hexose-6-phosphate dehydrogenase (H6PD), which physically interacts with 11beta-HSD1 at the luminal surface of the endoplasmic reticulum (ER) membrane and generates reduced nicotinamide adenine dinucleotide phosphate for the reduction of glucocorticoids. NADP 270-313 RNA, U1 small nuclear 1 Homo sapiens 29-84 18778749-2 2009 A NADPH/NADP(+) ratio of ten or higher was required for efficient microsomal 11beta-HSD1 reductase activity. NADP 2-7 RNA, U1 small nuclear 1 Homo sapiens 77-88 19416720-2 2009 An enzyme activity was observed in nuclear protein fractions that utilized either NADP(+) or NAD(+), but not NADPH and NADH, as pyridine nucleotide cofactor with K(m) values of 12+/-2 and 390+/-2microM, compared to the K(m) for microsomal 11betaHSD1 of 43+/-8 and 264+/-24microM, respectively. NADP 82-89 RNA, U1 small nuclear 1 Homo sapiens 228-249 19416720-4 2009 The K(cat) values for nuclear activity with NADP(+) was 1687nmol/min/mg/micromol, compared to 755nmol/min/mg/micromol for microsomal 11betaHSD1 activity. NADP 44-51 RNA, U1 small nuclear 1 Homo sapiens 122-143 19429452-2 2009 17beta-Hydroxysteroid dehydrogenase type 1 (17betaHSD1) catalyses the NADPH-dependent E2-formation from estrone (E1). NADP 70-75 RNA, U1 small nuclear 1 Homo sapiens 0-54 18778749-8 2009 11beta-HSD1 dehydrogenase activity was observed in H4IIE cells only at subphysiological glucose levels, indicating a highly efficient supply of substrate for H6PDH and NADPH generation in the ER-lumen. NADP 168-173 RNA, U1 small nuclear 1 Homo sapiens 0-11 19121282-2 2009 Hexose-6-phosphate dehydrogenase (H6PDH) is believed to supply NADPH for the reductase activity of 11beta-HSD1 in the lumen of the endoplasmic reticulum (ER), where the two enzymes are co-localized. NADP 63-68 RNA, U1 small nuclear 1 Homo sapiens 77-110 19010388-2 2009 We recently showed that the ratios of cortisone to cortisol and 7-keto- to 7-hydroxy-neurosteroids are regulated by 11beta-HSD1 and very much depend on coexpression with H6PDH, providing cosubstrate NADPH. NADP 199-204 RNA, U1 small nuclear 1 Homo sapiens 116-127 18778749-2 2009 A NADPH/NADP(+) ratio of ten or higher was required for efficient microsomal 11beta-HSD1 reductase activity. NADP 8-15 RNA, U1 small nuclear 1 Homo sapiens 77-88 18224704-1 2008 The 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) enzyme plays a crucial role in female hormonal regulation by catalysing the NADPH-dependent reduction of the less potent estrone E1 into the biologically active estradiol E2. NADP 137-142 RNA, U1 small nuclear 1 Homo sapiens 4-59 17017977-4 2006 Whereas the type 1 enzyme (11beta-HSD1) is in vitro a NADP(H)- dependent bidirectional enzyme, it reduces in most instances in vivo cortisone to active cortisol. NADP 54-61 RNA, U1 small nuclear 1 Homo sapiens 12-38 18209879-2 2007 11beta-hydroxysteroid dehydrogenase type 1 (11betaHSD1) is a NADPH-dependent enzyme highly expressed in the liver and adipose tissue. NADP 61-66 RNA, U1 small nuclear 1 Homo sapiens 0-54 17588937-3 2007 G6Pase-alpha shares its substrate (glucose 6-phosphate; G6P) with hexose-6-phosphate-dehydrogenase (H6PDH), a microsomal enzyme that regenerates NADPH within the endoplasmic reticulum lumen, thereby conferring reductase activity upon 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1). NADP 145-150 RNA, U1 small nuclear 1 Homo sapiens 234-289 17624766-1 2007 The human 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) catalyzes both the NADP(H)-dependent oxido-reduction of cortisol and cortisone and the inter-conversion of 7alpha- and 7beta-hydroxy-dehydroepiandrosterone (DHEA) through a 7-oxo-DHEA intermediate. NADP 86-93 RNA, U1 small nuclear 1 Homo sapiens 10-65 18336279-3 2008 The type 1 enzyme (11beta-HSD1) is a NADP(H)-dependent bidirectional enzyme in vitro and reduces cortisone to active cortisol in vivo. NADP 37-44 RNA, U1 small nuclear 1 Homo sapiens 4-30 15774558-6 2005 Because H6PDH and 11beta-HSD1 are coexpressed in the inner microsomal compartment of cells, we hypothesized that H6PDH may provide 11beta-HSD1 with NADPH, thus promoting oxo-reductase activity in vivo. NADP 148-153 RNA, U1 small nuclear 1 Homo sapiens 131-142 16356929-1 2006 The local generation of active glucocorticoid by NADPH-dependent, 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) oxoreductase activity, has emerged as an important factor in regulating hepatic glucose output and visceral adiposity. NADP 49-54 RNA, U1 small nuclear 1 Homo sapiens 110-121 15774558-3 2005 It has been proposed to be a NADPH regenerating system for steroid hormone and drug metabolism, specifically in determining the set point of 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) activity, the enzyme responsible for the activation and inactivation of glucocorticoids. NADP 29-34 RNA, U1 small nuclear 1 Homo sapiens 141-196 16892382-1 2006 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1), an oxidoreductase which has a preferential reductive activity using NADPH as cofactor, converts estrone to estradiol and is expressed in many steroidogenic tissues including breast and in malignant breast cells. NADP 126-131 RNA, U1 small nuclear 1 Homo sapiens 0-55 12604234-1 2003 17-beta-Hydroxysteroid dehydrogenase type 1 (17betaHSD1), also called estradiol dehydrogenase, catalyzes the NADPH-dependent reduction of the weak estrogen, estrone, into the more potent estrogen, 17-beta-estradiol. NADP 109-114 RNA, U1 small nuclear 1 Homo sapiens 0-55 7588404-3 1995 11 beta HSD1 is a low affinity NADP dependent enzyme, while 11 beta HSD2 is a high affinity NAD dependent species thought to be responsible for endowing specificity on the mineralocorticoid receptor and for protecting the fetus from high circulating levels of maternal glucocorticoids. NADP 31-35 RNA, U1 small nuclear 1 Homo sapiens 8-12 11604236-4 2001 Ishikawa cells contain an NAD dependent enzyme migrating at 41 kDa on Western blots, consistent with the presence of the glucocorticoid-inactivating enzyme 11 beta HSD2, while the NADP dependent 11 beta HSD1 is barely detectable. NADP 180-184 RNA, U1 small nuclear 1 Homo sapiens 203-207 9927655-3 1999 We present here the structure of the ternary complex of 17beta-HSD1 with the cofactor NADP+ and 3-hydroxyestra-1,3,5,7-tetraen-17-one (equilin), an equine estrogen used in estrogen replacement therapy. NADP 86-91 RNA, U1 small nuclear 1 Homo sapiens 56-67 9927655-7 1999 The orientation of the 17-keto group with respect to the nicotinamide ring of NADP+ and catalytic residues Tyr-155 and Ser-142 is different from that of E2 in the 17beta-HSD1-E2 complex. NADP 78-82 RNA, U1 small nuclear 1 Homo sapiens 163-174 10385414-2 1999 We have investigated the functional significance of local generation of cortisol within adipose tissue from inactive cortisone through the activity of the NADP(H)-dependent enzyme, 11beta-hydroxysteroid dehydrogenase type 1 (11betaHSD1). NADP 155-162 RNA, U1 small nuclear 1 Homo sapiens 181-235 9525918-2 1998 Here, we report the crystal structures of the H221L 17beta-HSD1 mutant complexed to NADP+ and estradiol and the H221L mutant/NAD+ and a H221Q mutant/estradiol complexes. NADP 84-89 RNA, U1 small nuclear 1 Homo sapiens 51-63 9120678-6 1996 The type 1 isoform (11 beta-HSD1) is a low-affinity reduced nicotinamide adenine dinucleotide phosphate (NADP) dependent dehydrogenase-oxoreductase which is expressed in predominantly glucocorticoid target tissues and the encoding sequence of which is normal in patients with AME. NADP 60-103 RNA, U1 small nuclear 1 Homo sapiens 4-32 9120678-6 1996 The type 1 isoform (11 beta-HSD1) is a low-affinity reduced nicotinamide adenine dinucleotide phosphate (NADP) dependent dehydrogenase-oxoreductase which is expressed in predominantly glucocorticoid target tissues and the encoding sequence of which is normal in patients with AME. NADP 105-109 RNA, U1 small nuclear 1 Homo sapiens 4-32