PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15012237-1 1998 Cytochrome P450-dependent monooxygenases are a large group of heme-containing enzymes, most of which catalyze NADPH- and O2-dependent hydroxylation reactions. NADP 110-115 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 11-15 9179987-2 1997 The construction of three-dimensional models of CYP2B isozymes from rat (CYP2B1), rabbit (CYP2B4) and man (CYP2B6), based on a multiple sequence alignment with CYP102, a unique eukaryotic-like bacterial P450 (in terms of possessing an NADPH-dependent FAD- and FMN-containing oxidoreductase redox partner) of known crystal structure, is reported. NADP 235-240 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 48-53 9179987-2 1997 The construction of three-dimensional models of CYP2B isozymes from rat (CYP2B1), rabbit (CYP2B4) and man (CYP2B6), based on a multiple sequence alignment with CYP102, a unique eukaryotic-like bacterial P450 (in terms of possessing an NADPH-dependent FAD- and FMN-containing oxidoreductase redox partner) of known crystal structure, is reported. NADP 235-240 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 107-113 8460938-7 1993 The monooxygenase activities of the purified human brain P450s were demonstrated with various substrates (aminopyrine, morphine, aniline, 7-ethoxycoumarin, and nifedipine) as examined in reconstituted systems consisting of purified human brain P450, purified rat brain NADPH cytochrome P450 reductase, deoxycholate, phospholipid, and NADPH. NADP 269-274 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 57-61 7788866-6 1995 Spectrally detectable P450 was also destroyed in microsomes and purified P450 in a reconstituted system in the presence of chlorophyllin and an NADPH-generating system. NADP 144-149 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 73-77 7819404-1 1994 Administration of a perfluorodecalin (PFD) emulsion, the liver cytochrome P-450 II B1/B2 inducer, to experimental animals is followed by a two-fold increase of the NADPH oxidation rate in liver microsomes. NADP 164-169 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 63-88 9046019-1 1997 In this paper we demonstrate that ascorbic acid specifically prevents NADPH-initiated cytochrome P450 (P450)-mediated microsomal lipid peroxidation in the absence of free iron. NADP 70-75 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 97-101 7788866-6 1995 Spectrally detectable P450 was also destroyed in microsomes and purified P450 in a reconstituted system in the presence of chlorophyllin and an NADPH-generating system. NADP 144-149 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 22-26 8323289-2 1993 Acetylation of 2.2 and 8.5 mol of lysine/mole of P450 by acetic anhydride led to 38.7 and 95% reductions, respectively, in benzphetamine demethylation activity by NADPH-dependent reconstituted P450/reductase complex, while modification of up to 8.5 mol of lysine/mol of P450 did not inhibit cumene hydroperoxide-supported P450-dependent benzphetamine demethylation. NADP 163-168 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 49-53 8323289-2 1993 Acetylation of 2.2 and 8.5 mol of lysine/mole of P450 by acetic anhydride led to 38.7 and 95% reductions, respectively, in benzphetamine demethylation activity by NADPH-dependent reconstituted P450/reductase complex, while modification of up to 8.5 mol of lysine/mol of P450 did not inhibit cumene hydroperoxide-supported P450-dependent benzphetamine demethylation. NADP 163-168 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 193-197 8323289-2 1993 Acetylation of 2.2 and 8.5 mol of lysine/mole of P450 by acetic anhydride led to 38.7 and 95% reductions, respectively, in benzphetamine demethylation activity by NADPH-dependent reconstituted P450/reductase complex, while modification of up to 8.5 mol of lysine/mol of P450 did not inhibit cumene hydroperoxide-supported P450-dependent benzphetamine demethylation. NADP 163-168 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 193-197 8323289-2 1993 Acetylation of 2.2 and 8.5 mol of lysine/mole of P450 by acetic anhydride led to 38.7 and 95% reductions, respectively, in benzphetamine demethylation activity by NADPH-dependent reconstituted P450/reductase complex, while modification of up to 8.5 mol of lysine/mol of P450 did not inhibit cumene hydroperoxide-supported P450-dependent benzphetamine demethylation. NADP 163-168 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 193-197 3304205-1 1987 Of the family of P-450 cytochromes occurring in rabbit liver microsomes, only isozyme 3a (P-450ALC) is induced by alcohol administration and is effective in catalyzing the reaction: ethanol +02+NADPH+H+----acetaldehyde +2H2O+NADP+. NADP 194-199 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 17-22 16668240-3 1991 Evidence that cytochromes P-450 are involved in the detoxification of herbicides (chlorotoluron, primsulfuron, and diclofop) includes photoreversible CO inhibition of the reactions, and a requirement for O(2) and NADPH. NADP 213-218 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 26-31 2856164-8 1988 The RA metabolism in rat epidermal microsomes shows the typical characteristics of a cytochrome-P-450 (P450)-dependent enzyme system, i.e. a requirement for NADPH and oxygen and inhibition by CO and SKF-525A. NADP 157-162 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 103-107 1349603-2 1992 Mitochondrial ferredoxins mediate electron transfer from NADPH:ferredoxin oxidoreductase to cytochrome P450 enzymes. NADP 57-62 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 103-107 3304205-1 1987 Of the family of P-450 cytochromes occurring in rabbit liver microsomes, only isozyme 3a (P-450ALC) is induced by alcohol administration and is effective in catalyzing the reaction: ethanol +02+NADPH+H+----acetaldehyde +2H2O+NADP+. NADP 194-199 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 90-98 3304205-1 1987 Of the family of P-450 cytochromes occurring in rabbit liver microsomes, only isozyme 3a (P-450ALC) is induced by alcohol administration and is effective in catalyzing the reaction: ethanol +02+NADPH+H+----acetaldehyde +2H2O+NADP+. NADP 225-230 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 17-22 3304205-1 1987 Of the family of P-450 cytochromes occurring in rabbit liver microsomes, only isozyme 3a (P-450ALC) is induced by alcohol administration and is effective in catalyzing the reaction: ethanol +02+NADPH+H+----acetaldehyde +2H2O+NADP+. NADP 225-230 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 90-98 6489339-1 1984 The NADPH-supported reduction of cytochrome P-450 LM2 (liver microsomal isozyme 2) in reconstituted phospholipid vesicles in general exhibits two-exponential kinetics. NADP 4-9 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 44-49 6489339-0 1984 Mechanism of rate control of the NADPH-dependent reduction of cytochrome P-450 by lipids in reconstituted phospholipid vesicles. NADP 33-38 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 73-78 31001351-6 2019 CYP3A4 was more strongly inhibited by the extract in the presence of NADPH, suggesting that the extract may inhibit CYP2B6 and CYP3A4 via mechanism-based inactivation. NADP 69-74 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 116-122 6416251-3 1983 The NADPH-dependent reduction of P-450 LM2 has been studied both anaerobically and aerobically in solution state. NADP 4-9 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 33-38 4156173-2 1974 The involvement of cytochrome b5 in the NADPH-supported cytochrome P-450-dependent hydroxylation of chlorobenzene. NADP 40-45 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 67-72 29148818-0 2017 Dissociation Constants of Cytochrome P450 2C9/Cytochrome P450 Reductase Complexes in a Lipid Bilayer Membrane Depend on NADPH: A Single-Protein Tracking Study. NADP 120-125 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 37-41 28288493-4 2017 Furthermore, the inhibition was potentiated following preincubation with NADPH, indicating that G. cambogia extract is a time-dependent inhibitor of CYP2B6. NADP 73-78 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 149-155 26335194-3 2015 We evaluated the inhibitory effect of PRN on cytochrome P450 2B6 (CYP2B6) and found that PRN induced a time-, concentration-, and NADPH-dependent inactivation of CYP2B6 with the values of KI and kinact being 110.2 muM and 0.200 min(-1), respectively. NADP 130-135 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 45-64 26335194-3 2015 We evaluated the inhibitory effect of PRN on cytochrome P450 2B6 (CYP2B6) and found that PRN induced a time-, concentration-, and NADPH-dependent inactivation of CYP2B6 with the values of KI and kinact being 110.2 muM and 0.200 min(-1), respectively. NADP 130-135 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 66-72 26335194-3 2015 We evaluated the inhibitory effect of PRN on cytochrome P450 2B6 (CYP2B6) and found that PRN induced a time-, concentration-, and NADPH-dependent inactivation of CYP2B6 with the values of KI and kinact being 110.2 muM and 0.200 min(-1), respectively. NADP 130-135 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 162-168 25378064-6 2015 The loss of CYP2B6 activity required the presence of NADPH. NADP 53-58 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 12-18 19171677-1 2009 Cytochrome P450 (P450) enzymes typically require the presence of at least cytochrome P450 reductase (CPR) and NADPH to carry out the metabolism of xenobiotics. NADP 110-115 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 0-21 22685215-6 2012 Methadone inactivates CYP2B6 in a time-, concentration-, and NADPH-dependent manner with a K(I) = 10.0 muM and k(inact) = 0.027 min-1. NADP 61-66 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 22-28 22685215-7 2012 The loss of CYP2B6 activity in the presence of methadone and NADPH occurred with concomitant loss of the reduced CO spectrum of the P450. NADP 61-66 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 12-18 21659470-6 2011 Presteady-state measurements of the rate of electron transfer from NADPH-dependent cytochrome P450 reductase (CPR) to CYP2B6.8 using stopped-flow spectrophotometry revealed that CYP2B6.8 is incapable of accepting electrons from CPR. NADP 67-72 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 118-124 21659470-6 2011 Presteady-state measurements of the rate of electron transfer from NADPH-dependent cytochrome P450 reductase (CPR) to CYP2B6.8 using stopped-flow spectrophotometry revealed that CYP2B6.8 is incapable of accepting electrons from CPR. NADP 67-72 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 178-184 20849814-1 2010 Cytochrome P450 3A4 (CYP3A4), the major P450 present in human liver metabolizes approximately half the drugs in clinical use and requires electrons supplied from NADPH through NADPH-P450 reductase (POR, CPR). NADP 162-167 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 11-15 22085411-4 2012 We synthesized caged molecules of nicotinamide adenine dinucleotide phosphate (NADP(+)) and glucose 6-phosphate (G6P), which are involved in the generation of NADPH (cofactor of P450). NADP 34-77 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 178-182 22085411-4 2012 We synthesized caged molecules of nicotinamide adenine dinucleotide phosphate (NADP(+)) and glucose 6-phosphate (G6P), which are involved in the generation of NADPH (cofactor of P450). NADP 79-86 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 178-182 22085411-4 2012 We synthesized caged molecules of nicotinamide adenine dinucleotide phosphate (NADP(+)) and glucose 6-phosphate (G6P), which are involved in the generation of NADPH (cofactor of P450). NADP 159-164 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 178-182 21930824-2 2011 The inactivation of CYP2B6 by BPA is time-, concentration-, and NADPH-dependent and exhibits a K(I) of 2.8 muM, a k(inact) of 0.7 min(-1), and a t(1/2) of 1 min. NADP 64-69 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 20-26 20851096-9 2011 Catalytic turnover of P450(cin) with either of these alternative substrates (camphane or cinane) revealed that in the absence of the ethereal oxygen there was still a significant amount of coupling of the NADPH-reducing equivalents to the formation of oxidised product. NADP 205-210 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 22-26 20849814-1 2010 Cytochrome P450 3A4 (CYP3A4), the major P450 present in human liver metabolizes approximately half the drugs in clinical use and requires electrons supplied from NADPH through NADPH-P450 reductase (POR, CPR). NADP 162-167 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 40-44 17693640-8 2007 Under steady-state conditions at all cyt b(5):cyt P450 molar ratios examined, cyt b(5) inhibits the rate of NADPH consumption. NADP 108-113 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 50-54 16959210-14 2006 In addition, the C98W mutant exhibited a 41% decrease in the maximum electron flow rate between P450 and reductase as measured by reduced nicotinamide adenine dinucleotide phosphate consumption at a saturating reductase concentration. NADP 138-181 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 96-114 17213193-7 2007 The resolution of the fractional contributions of binding intermediates of CYP3A4 into experimentally observed overall spin shift and the rates of steady-state NADPH oxidation and product formation provide new detailed insight into the mechanisms of cooperativity and allosteric regulation in this human cytochrome P450. NADP 160-165 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 315-319 16882766-4 2006 Five of the six trifluoroaryldiaziridines tested selectively inactivated P450 2B6 in the reconstituted system in a time-, concentration-, and NADPH-dependent manner as measured using the 7-ethoxy-4-(trifluoromethyl)coumarin O-deethylation assay. NADP 142-147 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 73-77 15774560-6 2005 Type II P450 enzymes, found in the endoplasmic reticulum, receive electrons from NADPH via P450 oxidoreductase (POR), which contains two flavin moieties. NADP 81-86 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 0-12 15081898-2 2004 tBMP inactivated p450 2B4 in a time-, concentration-, and NADPH-dependent manner. NADP 58-63 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 17-21 15652242-6 2005 The loss of CYP2B6 activity was NADPH-dependent and could not be restored by extensive dialysis. NADP 32-37 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 12-18 14563790-6 2004 Inhibition of CYP2B6 was time- and concentration-dependent, and as shown by dialysis experiments, it was irreversible and dependent on NADPH, suggesting a mechanism-based mode of action. NADP 135-140 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 14-20 15089095-1 2004 Cytochrome p450 (p450) 1A2 and NADPH-P450 reductase (NPR) catalyzed the oxidation of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), with consumption of NADPH. NADP 31-36 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 37-41 15089095-2 2004 The oxidation rate of NADPH by p450 1A2/NPR increased with time in the presence of IQ until depletion of NADPH. NADP 22-27 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 31-35 15089095-2 2004 The oxidation rate of NADPH by p450 1A2/NPR increased with time in the presence of IQ until depletion of NADPH. NADP 105-110 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 31-35 12725870-1 2003 Microsomal P450-mediated monooxygenase activity supported by NADPH requires an interaction between flavoprotein NADPH-cytochrome P450 reductase and cytochrome P450. NADP 61-66 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 11-15 12920169-7 2003 Contributions of cytochrome p450 (p450) enzymes to NNK oxidation were demonstrated by NADPH dependence, inhibition by carbon monoxide, and inhibition by the nonselective p450 inhibitors proadifen hydrochloride (SKF-525A) and 1-aminobenzotriazole (ABT), particularly in lung microsomes from high bioactivators. NADP 86-91 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 28-32 12920169-7 2003 Contributions of cytochrome p450 (p450) enzymes to NNK oxidation were demonstrated by NADPH dependence, inhibition by carbon monoxide, and inhibition by the nonselective p450 inhibitors proadifen hydrochloride (SKF-525A) and 1-aminobenzotriazole (ABT), particularly in lung microsomes from high bioactivators. NADP 86-91 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 34-38 12920169-7 2003 Contributions of cytochrome p450 (p450) enzymes to NNK oxidation were demonstrated by NADPH dependence, inhibition by carbon monoxide, and inhibition by the nonselective p450 inhibitors proadifen hydrochloride (SKF-525A) and 1-aminobenzotriazole (ABT), particularly in lung microsomes from high bioactivators. NADP 86-91 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 34-38 12485952-2 2003 PCP inactivated the 7-ethoxy-4-(trifluoromethyl)coumarin O-deethylation activity of p450 2B6 in a concentration-, time-, and NADPH-dependent manner and exhibited pseudo-first order kinetics. NADP 125-130 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 84-88 12527701-5 2003 Fluorometry was used to measure N-alkylPP formation following interaction of porphyrinogenic xenobiotics and NADPH with cDNA-expressed human P450 enzymes in microsomes from HLCL or BIICL. NADP 109-114 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 141-145 11684365-6 2001 The formation of neoantigenic trifluoroacetylated protein adducts by microsomal mixtures incubated with HCFC-123 and NADPH was catalysed primarily by CYP2E1 and to a lesser extent by CYP2C19, whereas, only trace levels of immunoreactive protein were seen with microsomes expressing CYP2B6 or CYP2C8. NADP 117-122 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 282-288