PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28260110-6 2017 FASN activity was measured by monitoring oxidation of nicotinamide adenine dinucleotide phosphate at a wavelength of 340 nm, and intracellular free fatty acid levels were detected using a Free Fatty Acid Quantification kit. NADP 54-97 fatty acid synthase Homo sapiens 0-4 28427229-0 2017 NADPH accumulation is responsible for apoptosis in breast cancer cells induced by fatty acid synthase inhibition. NADP 0-5 fatty acid synthase Homo sapiens 82-101 28427229-5 2017 Instead, NADPH accumulation resulting from FAS inhibition was found to stimulate NADPH oxidase to generate reactive oxygen species and highly associated with apoptosis induction. NADP 9-14 fatty acid synthase Homo sapiens 43-46 28427229-7 2017 Taken together, these data suggest that FAS plays a critical role in maintaining cellular redox homeostasis and its inhibition leads to NADPH accumulation-mediated apoptosis. NADP 136-141 fatty acid synthase Homo sapiens 40-43 28962653-5 2017 RESULTS: Using purinome capture media, we observed that HIV-1 infection increases intracellular levels of fatty acid synthase (FASN), a NADPH-using enzyme critical to the synthesis of de novo fatty acids. NADP 136-141 fatty acid synthase Homo sapiens 127-131 26680361-2 2016 FAS synthesizes long-chain fatty acids from three substrates: acetyl-CoA as a primer, malonyl-CoA as a 2 carbon donor, and NADPH for reduction. NADP 123-128 fatty acid synthase Homo sapiens 0-3 26586251-2 2016 The steps catalyzed by FAS involve the condensation of acetyl-CoA and malonyl-CoA moieties in the presence of NADPH until palmitate is formed. NADP 110-115 fatty acid synthase Homo sapiens 23-26 25924694-5 2015 The in vitro inhibitory activities on FAS of 22 extracts of spices were assessed by spectrophotometrically monitoring oxidation of NADPH at 340 nm. NADP 131-136 fatty acid synthase Homo sapiens 38-41 26364942-2 2015 2.3.1.85; FASN) is a multifunctional enzyme system that catalyzes the formation of fatty acids from acetyl-CoA, malonyl-CoA, and NADPH and plays a central role in lipid biosynthesis. NADP 129-134 fatty acid synthase Homo sapiens 10-14 24894151-6 2014 Intracellular FAS activity was measured by a spectrophotometer at 340 nm of NADPH absorption. NADP 76-81 fatty acid synthase Homo sapiens 14-17 25009654-6 2014 Intracellular FASN activity was calculated by measuring the absorption of NADPH via a spectrophotometer. NADP 74-79 fatty acid synthase Homo sapiens 14-18 24957042-4 2014 The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. NADP 86-129 fatty acid synthase Homo sapiens 4-7 24957042-4 2014 The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. NADP 86-129 fatty acid synthase Homo sapiens 21-24 24957042-4 2014 The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. NADP 131-136 fatty acid synthase Homo sapiens 4-7 24957042-4 2014 The FAS activity and FAS inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (NADPH) absorption. NADP 131-136 fatty acid synthase Homo sapiens 21-24 17168665-1 2006 The expression and activity of Fatty Acid Synthase (FASN; the sole enzyme capable of the reductive de novo synthesis of long-chain fatty acids from acetyl-CoA, malonyl-CoA, and nicotinamide adenine dinucleotide phosphate -NADPH-) is extremely low in nearly all nonmalignant adult tissues, whereas it is significantly up-regulated or activated in many cancer types, thus creating the potential for a large therapeutic index. NADP 177-220 fatty acid synthase Homo sapiens 31-50 19782152-5 2010 HER2 also establishes a positive bidirectional relationship with the key lipogenic enzyme Fatty Acid Synthase (FASN) that rapidly senses and respond to any disturbance in the flux of lipogenic substrates (e.g. NADPH and acetyl-CoA) and lipogenesis end-products (i.e. palmitate). NADP 210-215 fatty acid synthase Homo sapiens 90-109 19782152-5 2010 HER2 also establishes a positive bidirectional relationship with the key lipogenic enzyme Fatty Acid Synthase (FASN) that rapidly senses and respond to any disturbance in the flux of lipogenic substrates (e.g. NADPH and acetyl-CoA) and lipogenesis end-products (i.e. palmitate). NADP 210-215 fatty acid synthase Homo sapiens 111-115 22728958-6 2012 FASN activity determined by [13C16]palmitate synthesis was consistent with values obtained from beta-nicotinamide adenine dinucleotide 2"-phosphate (NADPH) oxidation assays. NADP 149-154 fatty acid synthase Homo sapiens 0-4 20074555-5 2010 Moreover, NADPH significantly enhanced the inactivation of FAS by low concentration of UA, but NADPH slightly decreased the inactivation of FAS by high concentration of UA. NADP 10-15 fatty acid synthase Homo sapiens 59-62 20074555-5 2010 Moreover, NADPH significantly enhanced the inactivation of FAS by low concentration of UA, but NADPH slightly decreased the inactivation of FAS by high concentration of UA. NADP 95-100 fatty acid synthase Homo sapiens 140-143 20074555-7 2010 The combination of NADPH and KR domain promotes the inhibitory effect of UA on FAS. NADP 19-24 fatty acid synthase Homo sapiens 79-82 18201766-6 2008 The corresponding Km and kcat values of FAS were 126 mM and 4.63 s(-1) for EAA; 8.7 microM and 4.09 s(-1) for NADPH. NADP 110-115 fatty acid synthase Homo sapiens 40-43 17168665-1 2006 The expression and activity of Fatty Acid Synthase (FASN; the sole enzyme capable of the reductive de novo synthesis of long-chain fatty acids from acetyl-CoA, malonyl-CoA, and nicotinamide adenine dinucleotide phosphate -NADPH-) is extremely low in nearly all nonmalignant adult tissues, whereas it is significantly up-regulated or activated in many cancer types, thus creating the potential for a large therapeutic index. NADP 177-220 fatty acid synthase Homo sapiens 52-56 17168665-1 2006 The expression and activity of Fatty Acid Synthase (FASN; the sole enzyme capable of the reductive de novo synthesis of long-chain fatty acids from acetyl-CoA, malonyl-CoA, and nicotinamide adenine dinucleotide phosphate -NADPH-) is extremely low in nearly all nonmalignant adult tissues, whereas it is significantly up-regulated or activated in many cancer types, thus creating the potential for a large therapeutic index. NADP 222-227 fatty acid synthase Homo sapiens 31-50 17168665-1 2006 The expression and activity of Fatty Acid Synthase (FASN; the sole enzyme capable of the reductive de novo synthesis of long-chain fatty acids from acetyl-CoA, malonyl-CoA, and nicotinamide adenine dinucleotide phosphate -NADPH-) is extremely low in nearly all nonmalignant adult tissues, whereas it is significantly up-regulated or activated in many cancer types, thus creating the potential for a large therapeutic index. NADP 222-227 fatty acid synthase Homo sapiens 52-56 7567999-2 1995 The HepG2 FAS has a specific activity of 600 nmol of NADPH oxidized per min per mg, which is about half that of chicken liver FAS. NADP 53-58 fatty acid synthase Homo sapiens 10-13 14999013-4 2004 The results showed that morin inhibited the overall reaction of FAS competitively with Ac-CoA, noncompetitively with Mal-CoA and in a mixed manner with NADPH. NADP 152-157 fatty acid synthase Homo sapiens 64-67 8962082-6 1996 The maltose-binding protein-human FAS (MBP-hFAS) catalyzed palmitate synthesis from acetyl-CoA, malonyl-CoA, and NADPH and exhibited all of the partial activities of FAS at levels comparable with those of the native human enzyme purified from HepG2 cells. NADP 113-118 fatty acid synthase Homo sapiens 34-37 8962082-6 1996 The maltose-binding protein-human FAS (MBP-hFAS) catalyzed palmitate synthesis from acetyl-CoA, malonyl-CoA, and NADPH and exhibited all of the partial activities of FAS at levels comparable with those of the native human enzyme purified from HepG2 cells. NADP 113-118 fatty acid synthase Homo sapiens 44-47 11064249-3 2000 In cultured keratinocytes, the FAS activity, assayed by measuring the oxidation of NADPH, was slightly increased by hydrocortisone or testosterone, but not influenced by thyroid hormone, estrogen, progesterone or insulin. NADP 83-88 fatty acid synthase Homo sapiens 31-34 8022791-4 1994 Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA-dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. NADP 35-40 fatty acid synthase Homo sapiens 6-25 8022791-4 1994 Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA-dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. NADP 35-40 fatty acid synthase Homo sapiens 305-324 8022791-4 1994 Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA-dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. NADP 35-40 fatty acid synthase Homo sapiens 305-324 8022791-4 1994 Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA-dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. NADP 197-202 fatty acid synthase Homo sapiens 6-25 8022791-4 1994 Tumor fatty acid synthase oxidized NADPH in a malonyl-CoA-dependent fashion and synthesized fatty acids composed of 80% palmitate, 10% myristate, and 10% stearate from acetyl-CoA, malonyl-CoA, and NADPH with a specific activity of 624 nmol of NADPH oxidized per min per mg. Tumor cell lines with elevated fatty acid synthase showed commensurate increases in incorporation of [U-14C]acetate into acylglycerols demonstrating that fatty acid synthase increases occur in the context of overall increases in endogenous fatty acid synthesis. NADP 197-202 fatty acid synthase Homo sapiens 6-25 32275951-8 2020 The identified compounds were tested for their inhibitor potencies in vitro using a substrate-based activity assay monitoring FASN-dependent NADPH consumption in LNCaP prostate cancer cell preparation, in parallel with selected reference inhibitors, including orlistat (THL), GSK2194069, GSK837149A, platensimycin and BI-99179. NADP 141-146 fatty acid synthase Homo sapiens 126-130 32275951-9 2020 LNCaP lysate supernatant was validated as a reliable native preparation to monitor FASN-dependent NADPH consumption as opposed to human glioma GAMG cells, whereas FASN enrichment was a prerequisite for accurate assays. NADP 98-103 fatty acid synthase Homo sapiens 83-87 31164796-7 2019 Intracellular FAS activity was measured by a spectrophotometer at 340 nm of NADPH absorption. NADP 76-81 fatty acid synthase Homo sapiens 14-17 23214141-1 2012 Fatty acid synthase (FAS)--enzyme present in many tissues, catalyses synthesis of palmitate from malonyl-CoA, acetyl-CoA and NADPH. NADP 125-130 fatty acid synthase Homo sapiens 0-19 23214141-1 2012 Fatty acid synthase (FAS)--enzyme present in many tissues, catalyses synthesis of palmitate from malonyl-CoA, acetyl-CoA and NADPH. NADP 125-130 fatty acid synthase Homo sapiens 21-24 3530833-1 1986 Fatty acid synthase catalyzes the reduction of one of the carbonyl groups in phenylglyoxal and 2,3-butanedione using NADPH as the reductant. NADP 117-122 fatty acid synthase Homo sapiens 0-19