PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9603372-2 1998 In the light microscope, the staining pattern with the nNOS antibody closely resembled that seen following histochemical processing with nicotinamide adenine dinucleotide phosphate diaphorase. NADP 137-180 nitric oxide synthase 1 Rattus norvegicus 55-59 9886024-2 1999 In the cochlea, nNOS was identified in spiral ganglion cells by using nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry and in situ hybridization. NADP 70-113 nitric oxide synthase 1 Rattus norvegicus 16-20 9886024-2 1999 In the cochlea, nNOS was identified in spiral ganglion cells by using nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry and in situ hybridization. NADP 115-120 nitric oxide synthase 1 Rattus norvegicus 16-20 9073164-6 1997 Combined NADPH-d histochemistry and in situ hybridization using glutamic acid decarboxylase (GAD) cRNA probe revealed that nNOS-expressing Purkinje cells showed fewer GAD mRNA signals than those in normal Purkinje cells. NADP 9-14 nitric oxide synthase 1 Rattus norvegicus 123-127 28887183-6 2017 Parallel studies using NADPH-diaphorase histochemistry to assay nNOS activity produced highly complementary outcomes. NADP 23-28 nitric oxide synthase 1 Rattus norvegicus 64-68 8702805-3 1996 Calmodulin binding to nNOS increases the rate of electron transfer from NADPH into its flavins, triggers electron transfer from flavins to the heme, activates NO synthesis, and increases reduction of artificial electron acceptors such as cytochrome c. To investigate what role the reductase domain plays in calmodulin"s activation of these functions, we overexpressed a form of the nNOS reductase domain (amino acids 724-1429) in the yeast Pichia pastoris that for the first time exhibits a complete calmodulin response. NADP 72-77 nitric oxide synthase 1 Rattus norvegicus 22-26 8702805-8 1996 Calmodulin binding to the recombinant reductase protein increased its rate of NADPH-dependent flavin reduction and its rate of electron transfer to cytochrome c, FeCN6, or dichlorophenolindophenol to fully match the rate increases achieved when calmodulin bound to native full-length nNOS. NADP 78-83 nitric oxide synthase 1 Rattus norvegicus 284-288 7556767-4 1995 It can thus be concluded that the modified NADPH-d procedure allows for a more specific detection of the histochemical nNOS activity than the conventional method. NADP 43-48 nitric oxide synthase 1 Rattus norvegicus 119-123 7512584-5 1994 Diaphorase staining combined with demonstration of nNOS by polyclonal antibodies revealed that NADPH-d activity of neuron somata is associated with nNOS immunoreactivity. NADP 95-100 nitric oxide synthase 1 Rattus norvegicus 51-55 7512584-5 1994 Diaphorase staining combined with demonstration of nNOS by polyclonal antibodies revealed that NADPH-d activity of neuron somata is associated with nNOS immunoreactivity. NADP 95-100 nitric oxide synthase 1 Rattus norvegicus 148-152 7512584-6 1994 It is concluded that neuron somata exhibit NADPH-d activity of nNOS. NADP 43-48 nitric oxide synthase 1 Rattus norvegicus 63-67 7512584-7 1994 NADPH-d activity of nNOS did not utilize beta-NADH or alpha-NADPH. NADP 0-5 nitric oxide synthase 1 Rattus norvegicus 20-24 22921486-9 2012 Furthermore, NADPH-diaphorase staining of nNOS in the PVN infected with lenti-nNOS induced a visible increase in nNOS expression compared with contralateral non-injected side up to three weeks. NADP 13-18 nitric oxide synthase 1 Rattus norvegicus 42-46 22921486-9 2012 Furthermore, NADPH-diaphorase staining of nNOS in the PVN infected with lenti-nNOS induced a visible increase in nNOS expression compared with contralateral non-injected side up to three weeks. NADP 13-18 nitric oxide synthase 1 Rattus norvegicus 78-82 22921486-9 2012 Furthermore, NADPH-diaphorase staining of nNOS in the PVN infected with lenti-nNOS induced a visible increase in nNOS expression compared with contralateral non-injected side up to three weeks. NADP 13-18 nitric oxide synthase 1 Rattus norvegicus 78-82 16916949-5 2006 Inhibition of the NADPH-consuming enzyme neuronal nitric oxide synthase (nNOS) with l-N(G)-nitro-l-arginine methyl ester or with N(G)-monomethyl-l-arginine(G) doubled succinate-stimulated insulin secretion in rat islets, suggesting that succinate- and nNOS-derived signals interact to regulate insulin secretion. NADP 18-23 nitric oxide synthase 1 Rattus norvegicus 41-71 20405262-0 2010 Activation of neuronal nitric oxide synthase in cerebellum of chronic hepatic encephalopathy rats is associated with up-regulation of NADPH-producing pathway. NADP 134-139 nitric oxide synthase 1 Rattus norvegicus 14-44 20405262-3 2010 This article describes profiles of NADPH-dependent neuronal nitric oxide synthase (nNOS) and those of glutathione peroxidase and glutathione reductase (GR) vis-a-vis regulation of NADPH-producing pathway in the cerebellum of CLF rats induced by administration of thioacetamide (100 mg kg-1 b.w., i.p.) NADP 35-40 nitric oxide synthase 1 Rattus norvegicus 51-81 20405262-3 2010 This article describes profiles of NADPH-dependent neuronal nitric oxide synthase (nNOS) and those of glutathione peroxidase and glutathione reductase (GR) vis-a-vis regulation of NADPH-producing pathway in the cerebellum of CLF rats induced by administration of thioacetamide (100 mg kg-1 b.w., i.p.) NADP 35-40 nitric oxide synthase 1 Rattus norvegicus 83-87 22722929-0 2012 Control of electron transfer and catalysis in neuronal nitric-oxide synthase (nNOS) by a hinge connecting its FMN and FAD-NADPH domains. NADP 122-127 nitric oxide synthase 1 Rattus norvegicus 78-82 21627848-0 2011 (-)-Epigallocatechin gallate attenuates NADPH-d/nNOS expression in motor neurons of rats following peripheral nerve injury. NADP 40-45 nitric oxide synthase 1 Rattus norvegicus 48-52 21627848-8 2011 RESULTS: In rats treated with high dosages of EGCG (25 or 50 mg/kg), NADPH-d/nNOS reactivity and cell death of the motor neurons were significantly decreased. NADP 69-74 nitric oxide synthase 1 Rattus norvegicus 77-81 21627848-9 2011 CONCLUSIONS: The present evidence indicated that EGCG can reduce NADPH-d/nNOS reactivity and thus may enhance motor neuron survival time following peripheral nerve injury. NADP 65-70 nitric oxide synthase 1 Rattus norvegicus 73-77 16916949-5 2006 Inhibition of the NADPH-consuming enzyme neuronal nitric oxide synthase (nNOS) with l-N(G)-nitro-l-arginine methyl ester or with N(G)-monomethyl-l-arginine(G) doubled succinate-stimulated insulin secretion in rat islets, suggesting that succinate- and nNOS-derived signals interact to regulate insulin secretion. NADP 18-23 nitric oxide synthase 1 Rattus norvegicus 73-77 16916949-5 2006 Inhibition of the NADPH-consuming enzyme neuronal nitric oxide synthase (nNOS) with l-N(G)-nitro-l-arginine methyl ester or with N(G)-monomethyl-l-arginine(G) doubled succinate-stimulated insulin secretion in rat islets, suggesting that succinate- and nNOS-derived signals interact to regulate insulin secretion. NADP 18-23 nitric oxide synthase 1 Rattus norvegicus 252-256 11788407-6 2002 In Ad.nNOS-treated rats, the local expression of nNOS within the PVN was confirmed by histochemistry for NADPH-diaphorase-positive neurons. NADP 105-110 nitric oxide synthase 1 Rattus norvegicus 6-10 15652701-6 2005 The present results showed that about 38% of the neurons in the NG displayed NADPH-d/nNOS positive [NADPH-d/nNOS(+)] in normoxic rats. NADP 77-82 nitric oxide synthase 1 Rattus norvegicus 108-112 15652701-7 2005 In SHE rats, a peak in the percentage (71%) and staining intensity (230%) of NADPH-d/nNOS(+) nodose neurons at 0 day, which then gradually decreased at 7-60 days. NADP 77-82 nitric oxide synthase 1 Rattus norvegicus 85-89 15223068-4 2004 It was found that purified nNOS was capable of reducing TNT, with a specific activity of 3900 nmol of NADPH oxidized/mg/min, but this reaction required CaCl(2)/calmodulin (CaM). NADP 102-107 nitric oxide synthase 1 Rattus norvegicus 27-31 14746923-7 2004 The present results show a significant increase in the expression of NADPH-d/nNOS reactivity in neurons of the NG at various time intervals following hypoxia. NADP 69-74 nitric oxide synthase 1 Rattus norvegicus 77-81 12663061-4 2003 Quantitative evaluation revealed that about 43% of neurons in the NG showed positive response for NADPH-d/nNOS and NMDAR1 reactivities. NADP 98-103 nitric oxide synthase 1 Rattus norvegicus 106-110 12663061-5 2003 However, in animals subjected to hypoxia, both the percentage and the staining intensity of NADPH-d/nNOS and NMDAR1 labeled neurons were drastically increased. NADP 92-97 nitric oxide synthase 1 Rattus norvegicus 100-104 12663061-6 2003 The percentage of NADPH-d/nNOS and NMDAR1-immunoreactive neurons in the NG was raised to 68% as well as 77%, respectively, following 2 and 4 h of hypoxic exposure. NADP 18-23 nitric oxide synthase 1 Rattus norvegicus 26-30 11814404-1 2002 The neuronal isoform of nitric oxide synthase (nNOS), a NADPH-dependent diaphorase, is considered to play a role in motoneuron death induced by sciatic nerve transection in neonatal rats. NADP 56-61 nitric oxide synthase 1 Rattus norvegicus 47-51 16738031-2 2006 Although the time- and NADPH-dependent inhibition of nNOS has been reported in studies where guanabenz was incubated with crude cytosolic preparations of nNOS, the exact mechanism for inhibition is not known. NADP 23-28 nitric oxide synthase 1 Rattus norvegicus 53-57 16738031-2 2006 Although the time- and NADPH-dependent inhibition of nNOS has been reported in studies where guanabenz was incubated with crude cytosolic preparations of nNOS, the exact mechanism for inhibition is not known. NADP 23-28 nitric oxide synthase 1 Rattus norvegicus 154-158 12079493-1 2002 The reduction by NADPH of the FAD and FMN redox centres in the isolated flavin reductase domain of calmodulin-bound rat neuronal nitric oxide synthase (nNOS) has been studied by anaerobic stopped-flow spectroscopy using absorption and fluorescence detection. NADP 17-22 nitric oxide synthase 1 Rattus norvegicus 120-150 12079493-1 2002 The reduction by NADPH of the FAD and FMN redox centres in the isolated flavin reductase domain of calmodulin-bound rat neuronal nitric oxide synthase (nNOS) has been studied by anaerobic stopped-flow spectroscopy using absorption and fluorescence detection. NADP 17-22 nitric oxide synthase 1 Rattus norvegicus 152-156 12079493-8 2002 Unlike for the functionally related cytochrome P450 reductase, transfer of the first hydride equivalent from NADPH to nNOS reductase does not generate the flavin di-semiquinoid state. NADP 109-114 nitric oxide synthase 1 Rattus norvegicus 118-122 12071470-8 2002 Following hypoxic exposure, both the percentage and the staining intensity of NADPH-d/nNOS positive neurons in the NG were markedly increased, but these were reduced in longer surviving animals. NADP 78-83 nitric oxide synthase 1 Rattus norvegicus 86-90 11788407-6 2002 In Ad.nNOS-treated rats, the local expression of nNOS within the PVN was confirmed by histochemistry for NADPH-diaphorase-positive neurons. NADP 105-110 nitric oxide synthase 1 Rattus norvegicus 49-53 11325964-1 2001 Neuronal nitric oxide synthase (nNOS) is composed of an oxygenase domain that binds heme, (6R)-tetrahydrobiopterin, and Arg, coupled to a reductase domain that binds FAD, FMN, and NADPH. NADP 180-185 nitric oxide synthase 1 Rattus norvegicus 0-30 11427300-1 2001 Motoneuron death induced by sciatic nerve transection in neonatal rats has been related to induction of the neuronal isoform of nitric oxide synthase (nNOS), a diaphorase of which one of the cofactors is nicotinamide adenine dinucleotide phosphate (NADPH). NADP 204-247 nitric oxide synthase 1 Rattus norvegicus 151-155 11427300-1 2001 Motoneuron death induced by sciatic nerve transection in neonatal rats has been related to induction of the neuronal isoform of nitric oxide synthase (nNOS), a diaphorase of which one of the cofactors is nicotinamide adenine dinucleotide phosphate (NADPH). NADP 249-254 nitric oxide synthase 1 Rattus norvegicus 151-155 11325964-1 2001 Neuronal nitric oxide synthase (nNOS) is composed of an oxygenase domain that binds heme, (6R)-tetrahydrobiopterin, and Arg, coupled to a reductase domain that binds FAD, FMN, and NADPH. NADP 180-185 nitric oxide synthase 1 Rattus norvegicus 32-36 11106494-3 2000 We found in this study using nNOS purified from an Escherichia coli expression system that the ratio of the amount of OH-Arg to L-Cit (OH-Arg/L-Cit) increased to about 1 at low concentration of NADPH. NADP 194-199 nitric oxide synthase 1 Rattus norvegicus 29-33 11087959-6 2001 The increased levels of the nNOS mRNA in the PVN and SON were confirmed by NADPH-diaphorase histochemical staining. NADP 75-80 nitric oxide synthase 1 Rattus norvegicus 28-32 11166699-5 2001 Basal forebrain cholinergic hypofunction resulted in reduced catalytic and substrate binding activity of nNOS in a number of hippocampal and neocortical subregions 7 days after lesion as revealed by NADPH-diaphorase enzyme histochemistry and quantitative autoradiography of [3H]L-N(G)-nitro-arginine binding, respectively. NADP 199-204 nitric oxide synthase 1 Rattus norvegicus 105-109 11106494-4 2000 In one cycle of the nNOS reaction, the decrease in NADPH concentration was found to reduce the rates of two monooxygenase reactions but had little effect on the rate constant of OH-Arg dissociation from the enzyme. NADP 51-56 nitric oxide synthase 1 Rattus norvegicus 20-24