PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30077972-5 2018 Using thioflavin fluorescence, CD, and light scattering analysis along with atomic force microscopy imaging, we found that the temperature-dependent aggregation of beta2m markedly depends on NaCl concentration. thioflavin T 6-16 beta-2-microglobulin Homo sapiens 164-170 31495783-5 2019 Using thioflavin T assays, circular dichroism spectroscopy, and transmission electron microscopy analyses, we found that beta2m efficiently forms amyloid fibrils even at neutral pH by heating with agitation at high-salt conditions. thioflavin T 6-18 beta-2-microglobulin Homo sapiens 121-127 27345358-2 2016 When we induced the ultrasonication-dependent fibrillation of beta2-microglobulin and insulin monitored by amyloid-specific thioflavin T (ThT) fluorescence, both proteins showed a significant decrease in ThT fluorescence after the burst-phase increase. thioflavin T 124-136 beta-2-microglobulin Homo sapiens 62-81 30223436-0 2018 Structural Features of Amyloid Fibrils Formed from the Full-Length and Truncated Forms of Beta-2-Microglobulin Probed by Fluorescent Dye Thioflavin T. thioflavin T 137-149 beta-2-microglobulin Homo sapiens 90-110 30223436-5 2018 Spectroscopic analysis of the obtained samples allowed us to detect one binding mode (type) of ThT interaction with all the studied variants of beta2M amyloid fibrils with affinity ~104 M-1. thioflavin T 95-98 beta-2-microglobulin Homo sapiens 144-150 30223436-8 2018 The observed differences in the ThT-beta2M fibrils binding parameters and characteristics of the bound dye allowed to prove not only the difference of the DeltaN10beta2m fibrils from other beta2M fibrils (that can be detected visually, for example, by transmission electron microscopy (TEM), but also the differences between beta2m and DeltaN6beta2m fibrils (that can not be unequivocally confirmed by other approaches). thioflavin T 32-35 beta-2-microglobulin Homo sapiens 36-42 30223436-8 2018 The observed differences in the ThT-beta2M fibrils binding parameters and characteristics of the bound dye allowed to prove not only the difference of the DeltaN10beta2m fibrils from other beta2M fibrils (that can be detected visually, for example, by transmission electron microscopy (TEM), but also the differences between beta2m and DeltaN6beta2m fibrils (that can not be unequivocally confirmed by other approaches). thioflavin T 32-35 beta-2-microglobulin Homo sapiens 189-195 30223436-8 2018 The observed differences in the ThT-beta2M fibrils binding parameters and characteristics of the bound dye allowed to prove not only the difference of the DeltaN10beta2m fibrils from other beta2M fibrils (that can be detected visually, for example, by transmission electron microscopy (TEM), but also the differences between beta2m and DeltaN6beta2m fibrils (that can not be unequivocally confirmed by other approaches). thioflavin T 32-35 beta-2-microglobulin Homo sapiens 163-169 27345358-2 2016 When we induced the ultrasonication-dependent fibrillation of beta2-microglobulin and insulin monitored by amyloid-specific thioflavin T (ThT) fluorescence, both proteins showed a significant decrease in ThT fluorescence after the burst-phase increase. thioflavin T 138-141 beta-2-microglobulin Homo sapiens 62-81 27345358-2 2016 When we induced the ultrasonication-dependent fibrillation of beta2-microglobulin and insulin monitored by amyloid-specific thioflavin T (ThT) fluorescence, both proteins showed a significant decrease in ThT fluorescence after the burst-phase increase. thioflavin T 204-207 beta-2-microglobulin Homo sapiens 62-81 26063798-3 2015 When ultrasonication was used to accelerate the spontaneous fibrillation of beta2m at pH 2.0, the effects observed depended on ultrasonic power; although stronger ultrasonic power effectively accelerated fibrillation, excessively strong ultrasonic power decreased the amount of fibrils formed, as monitored by thioflavin T fluorescence. thioflavin T 310-322 beta-2-microglobulin Homo sapiens 76-82 26420657-7 2015 Furthermore, the crystal structure of DimC33 in complex with the amyloid-specific dye Thioflavin-T pinpoints a second interface, which likely participates in the first steps of beta2m aggregation. thioflavin T 86-98 beta-2-microglobulin Homo sapiens 177-183 24243599-3 2013 In this paper, we have investigated the ability of covalently linked long-chain fatty acids in modulating the self-assembly of an aromatic amino acid-rich highly amyloidogenic sequence derived from the amino acid region 59-71 of human beta2-microglobulin by thioflavin T (ThT) fluorescence microscopy, circular dichroism, and fluorescence spectroscopy. thioflavin T 258-270 beta-2-microglobulin Homo sapiens 235-254 24243599-3 2013 In this paper, we have investigated the ability of covalently linked long-chain fatty acids in modulating the self-assembly of an aromatic amino acid-rich highly amyloidogenic sequence derived from the amino acid region 59-71 of human beta2-microglobulin by thioflavin T (ThT) fluorescence microscopy, circular dichroism, and fluorescence spectroscopy. thioflavin T 272-275 beta-2-microglobulin Homo sapiens 235-254 20826442-6 2010 Here, we report the co-crystal structures of ThT with two alternative states of beta-2 microglobulin (beta2m); one monomeric, the other an amyloid-like oligomer. thioflavin T 45-48 beta-2-microglobulin Homo sapiens 80-100 23645685-1 2013 The interaction at neutral pH between wild-type and a variant form (R3A) of the amyloid fibril-forming protein beta2-microglobulin (beta2m) and the molecular chaperone alphaB-crystallin was investigated by thioflavin T fluorescence, NMR spectroscopy, and mass spectrometry. thioflavin T 206-218 beta-2-microglobulin Homo sapiens 111-130 23645685-1 2013 The interaction at neutral pH between wild-type and a variant form (R3A) of the amyloid fibril-forming protein beta2-microglobulin (beta2m) and the molecular chaperone alphaB-crystallin was investigated by thioflavin T fluorescence, NMR spectroscopy, and mass spectrometry. thioflavin T 206-218 beta-2-microglobulin Homo sapiens 132-138 21388222-3 2011 We found that freshly polymerized beta2m fibrils at 0.1-0.3 mg/mL concentration completely dissociated to monomers upon 10 min incubation at 99 C. Fibril depolymerization was followed by thioflavin-T fluorescence and circular dichroism spectroscopy at various temperatures. thioflavin T 188-200 beta-2-microglobulin Homo sapiens 34-40 20826442-6 2010 Here, we report the co-crystal structures of ThT with two alternative states of beta-2 microglobulin (beta2m); one monomeric, the other an amyloid-like oligomer. thioflavin T 45-48 beta-2-microglobulin Homo sapiens 102-108 10913285-2 2000 Using Congo red and thioflavin-T binding, electron microscopy, and X-ray fiber diffraction, we have determined conditions under which recombinant monomeric beta(2)m spontaneously associates to form fibrils in vitro. thioflavin T 20-32 beta-2-microglobulin Homo sapiens 156-164 16981683-3 2006 First, seed-dependent fibril growth of beta2-microglobulin (beta2-m) and amyloid beta peptide was visualized in real time at the single fibril level using total internal reflection fluorescence microscopy combined with the binding of thioflavin T, an amyloid-specific fluorescence dye. thioflavin T 234-246 beta-2-microglobulin Homo sapiens 39-58 15766269-5 2005 The unfolding rate is 1 order of magnitude faster in DeltaK58-beta(2)m than in wt-beta(2)m, and at 37 degrees C the half-time for unfolding is more than 170-fold faster than at 15 degrees C. Conformational changes are also reflected by a very prominent Congo red binding of DeltaK58-beta(2)m at 37 degrees C, by the evolution of thioflavin T fluorescence, and by changes in intrinsic fluorescence. thioflavin T 329-341 beta-2-microglobulin Homo sapiens 62-70 15766269-5 2005 The unfolding rate is 1 order of magnitude faster in DeltaK58-beta(2)m than in wt-beta(2)m, and at 37 degrees C the half-time for unfolding is more than 170-fold faster than at 15 degrees C. Conformational changes are also reflected by a very prominent Congo red binding of DeltaK58-beta(2)m at 37 degrees C, by the evolution of thioflavin T fluorescence, and by changes in intrinsic fluorescence. thioflavin T 329-341 beta-2-microglobulin Homo sapiens 82-90 15766269-5 2005 The unfolding rate is 1 order of magnitude faster in DeltaK58-beta(2)m than in wt-beta(2)m, and at 37 degrees C the half-time for unfolding is more than 170-fold faster than at 15 degrees C. Conformational changes are also reflected by a very prominent Congo red binding of DeltaK58-beta(2)m at 37 degrees C, by the evolution of thioflavin T fluorescence, and by changes in intrinsic fluorescence. thioflavin T 329-341 beta-2-microglobulin Homo sapiens 82-90 15628856-3 2005 A 22-residue peptide of beta(2)-microglobulin, Ser20-Lys41 (L-K3 peptide), obtained by digestion with Acromobacter protease I, formed amyloid-like fibrils in 50% (v/v) 2,2,2-trifluoroethanol and 10 mM HCl at 25 degrees C, as confirmed by thioflavin T fluorescence, circular dichroism spectra, and atomic force microscopy images. thioflavin T 238-250 beta-2-microglobulin Homo sapiens 24-45 19010783-2 2009 At neutral pH, irradiation at 442 nm with a laser beam to excite ThT inhibited the fibril growth of beta(2)-microglobulin (beta2-m), a major component of amyloid fibrils deposited in patients with dialysis-related amyloidosis. thioflavin T 65-68 beta-2-microglobulin Homo sapiens 100-121 18753422-6 2008 To investigate their conformational change and amyloidogenicity, we measured circular dichroism spectra, the fluorescence intensity of tryptophan and thioflavin-T (ThT) of the recombinant beta(2)m. thioflavin T 150-162 beta-2-microglobulin Homo sapiens 188-196 18753422-6 2008 To investigate their conformational change and amyloidogenicity, we measured circular dichroism spectra, the fluorescence intensity of tryptophan and thioflavin-T (ThT) of the recombinant beta(2)m. thioflavin T 164-167 beta-2-microglobulin Homo sapiens 188-196 18753422-11 2008 EM showed that beta(2)m formed amorphous debris containing typical amyloid fibrils at 24 hours, when ThT fluorescence intensity was three-fold lower than that at six hours. thioflavin T 101-104 beta-2-microglobulin Homo sapiens 15-23 10611946-8 1999 During the incubation of fA beta 2M with native beta 2-m at 37 degrees C, the fluorescence of thioflavin T increased without a lag phase and proceeded to equilibrium. thioflavin T 94-106 beta-2-microglobulin Homo sapiens 28-35 35615856-9 2022 When HEK293T cells were transfected with inflammasome components NLRP3 or Pyrin, along with ASC, pro-caspase-1, pro-IL-1beta, and B2M, ThT fluorescence intensity increased. thioflavin T 135-138 beta-2-microglobulin Homo sapiens 130-133